##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937167_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 369331 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9013513623281 31.0 31.0 34.0 30.0 34.0 2 32.14109836433985 33.0 31.0 34.0 30.0 34.0 3 32.17991449404464 34.0 31.0 34.0 30.0 34.0 4 35.91072777535598 37.0 35.0 37.0 35.0 37.0 5 35.822535882446914 37.0 35.0 37.0 35.0 37.0 6 35.86849465655469 37.0 35.0 37.0 35.0 37.0 7 35.79822164941475 37.0 35.0 37.0 35.0 37.0 8 35.759329706956635 37.0 35.0 37.0 33.0 37.0 9 37.407344631238644 39.0 37.0 39.0 34.0 39.0 10-11 37.30182681659541 39.0 37.0 39.0 34.0 39.0 12-13 37.17888560667802 39.0 37.0 39.0 33.0 39.0 14-15 38.293287051452495 40.0 38.0 41.0 33.0 41.0 16-17 38.233879636423694 40.0 38.0 41.0 33.0 41.0 18-19 38.237038049879374 40.0 38.0 41.0 33.5 41.0 20-21 38.12954639605124 40.0 37.5 41.0 33.0 41.0 22-23 38.061618169067856 40.0 37.0 41.0 33.0 41.0 24-25 38.07391743449642 40.0 37.0 41.0 33.0 41.0 26-27 37.870808299330406 40.0 37.0 41.0 33.0 41.0 28-29 37.70462268263428 39.5 37.0 41.0 33.0 41.0 30-31 37.704152914323465 39.0 37.0 41.0 33.0 41.0 32-33 37.55668898630226 39.5 36.5 41.0 33.0 41.0 34-35 37.38604666274968 39.0 36.0 41.0 32.5 41.0 36-37 37.176370247826476 39.0 36.0 41.0 31.5 41.0 38-39 36.99757399189345 39.0 36.0 41.0 31.0 41.0 40-41 36.755447010946824 39.0 35.0 40.5 31.0 41.0 42-43 36.514660291175126 39.0 35.0 40.0 30.5 41.0 44-45 36.13371203608687 39.0 35.0 40.0 30.0 41.0 46-47 35.691937584443224 38.0 35.0 40.0 28.5 41.0 48-49 35.28339077954463 38.0 35.0 40.0 27.0 41.0 50-51 34.973565717472944 38.0 34.0 40.0 26.0 41.0 52-53 34.68147542448373 38.0 34.0 40.0 25.5 41.0 54-55 34.42298778060872 38.0 34.0 40.0 24.5 41.0 56-57 34.05277109151412 38.0 33.0 40.0 23.5 41.0 58-59 33.744233492449865 37.0 33.0 40.0 23.0 41.0 60-61 33.32772364085333 37.0 32.5 39.5 22.0 41.0 62-63 32.89023125597364 36.0 32.0 39.0 20.5 40.0 64-65 32.40722008171532 36.0 31.0 39.0 18.5 40.0 66-67 31.92049543634301 35.0 31.0 38.5 17.0 40.0 68-69 31.414227346201645 35.0 30.5 38.0 12.5 40.0 70-71 30.921763946162116 34.5 30.0 37.5 8.0 39.5 72-73 30.388197849625403 34.0 29.0 37.0 7.0 39.0 74-75 29.876131437653484 34.0 29.0 36.0 6.5 39.0 76-77 28.691446155345748 32.5 27.0 35.0 2.0 37.5 78-79 29.337462872057856 34.0 29.0 35.0 2.0 38.0 80-81 29.139881298889073 34.0 29.0 35.0 2.0 37.0 82-83 28.66763688940273 34.0 28.5 35.0 2.0 37.0 84-85 28.172566072168326 33.0 27.0 35.0 2.0 36.0 86-87 27.729016789817265 33.0 26.5 35.0 2.0 36.0 88-89 27.154622818014193 33.0 26.0 35.0 2.0 35.0 90-91 26.478058976906894 32.0 25.0 34.0 2.0 35.0 92-93 25.80321310694201 32.0 23.5 34.0 2.0 35.0 94-95 25.145504980627134 31.0 20.5 34.0 2.0 35.0 96-97 24.277221516742433 31.0 12.5 34.0 2.0 35.0 98-99 23.072780243196483 31.0 2.0 34.0 2.0 35.0 100-101 20.164776311763703 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12934049688258398 1101 2 0.21106181036829952 1101 3 0.017411944298032722 1101 4 0.16187697173715776 1101 5 0.13458109903187676 1101 6 0.17668854527914846 1101 7 0.06622793068032706 1101 8 0.14632975374875912 1101 9 0.07812813388274265 1101 10-11 0.151616118919371 1101 12-13 0.182010806890176 1101 14-15 0.3228615330284157 1101 16-17 0.26801554516402604 1101 18-19 0.17888121575077065 1101 20-21 0.09340380708609786 1101 22-23 0.02359519207325178 1101 24-25 4.1645879259988305E-4 1101 26-27 -0.20927683026337007 1101 28-29 -0.2535897287190281 1101 30-31 0.23200270605477868 1101 32-33 0.19327151085495586 1101 34-35 0.2481011832901885 1101 36-37 0.3576211379094971 1101 38-39 0.2571596963556928 1101 40-41 0.5805092441343405 1101 42-43 0.6135996481786279 1101 44-45 0.6309907019494574 1101 46-47 0.9899725775196302 1101 48-49 0.6406268654081018 1101 50-51 0.7949048000095473 1101 52-53 0.812765637524258 1101 54-55 0.7855049000694478 1101 56-57 0.6742984502572327 1101 58-59 0.7616814636377498 1101 60-61 1.0936489612628506 1101 62-63 1.0092065501999414 1101 64-65 1.1624457689790901 1101 66-67 0.9534280965333473 1101 68-69 0.6406639569372246 1101 70-71 1.0904758952576188 1101 72-73 0.8473652917675345 1101 74-75 1.012329105089826 1101 76-77 1.106399547871387 1101 78-79 1.0512869955054214 1101 80-81 1.181613374463435 1101 82-83 1.058162759211715 1101 84-85 1.260775161017886 1101 86-87 1.4393497686028063 1101 88-89 1.723244940487536 1101 90-91 1.6450765719655536 1101 92-93 1.6581474499433249 1101 94-95 1.6873727341858462 1101 96-97 1.7820049626157122 1101 98-99 1.9631256540779525 1101 100-101 2.2813683163389555 1102 1 0.2630672673496868 1102 2 0.30337536953268796 1102 3 0.22807753203544934 1102 4 0.1295044435082744 1102 5 -0.01539006751387717 1102 6 0.2652212014913644 1102 7 0.11252887753376228 1102 8 0.011807738011270885 1102 9 -0.03566634869626739 1102 10-11 0.06896409220657773 1102 12-13 0.1321777618913842 1102 14-15 0.10878011107406849 1102 16-17 0.17876205259562283 1102 18-19 0.49053597699116835 1102 20-21 0.16060900826919777 1102 22-23 0.250591211196344 1102 24-25 0.2877162523078667 1102 26-27 0.5082483292507334 1102 28-29 0.2479542562503667 1102 30-31 0.5070668837432564 1102 32-33 0.25647955817902357 1102 34-35 0.48223813289675377 1102 36-37 0.520093002038827 1102 38-39 0.3275528544560373 1102 40-41 0.02827651165298306 1102 42-43 0.1432981060538836 1102 44-45 0.20004449959441217 1102 46-47 0.05694757612446466 1102 48-49 0.21819583317044078 1102 50-51 0.21194351124715638 1102 52-53 0.10545775203957675 1102 54-55 0.07148161007931009 1102 56-57 0.015960931422476676 1102 58-59 0.14843342152230576 1102 60-61 0.3502434512827577 1102 62-63 0.019037827987013145 1102 64-65 0.3034971669365589 1102 66-67 0.18487469883630325 1102 68-69 0.24573399432770415 1102 70-71 0.3875906896099828 1102 72-73 0.48802371764694996 1102 74-75 0.4177414234855732 1102 76-77 0.3634428166549881 1102 78-79 0.5077438290340837 1102 80-81 0.6999667982036009 1102 82-83 0.6902153291095878 1102 84-85 0.8075248866350186 1102 86-87 0.6586142364860557 1102 88-89 0.8943342869912456 1102 90-91 0.9730966437701554 1102 92-93 0.8141155099169062 1102 94-95 1.0514206721288133 1102 96-97 0.8790481383881712 1102 98-99 0.9828765702377105 1102 100-101 1.7880906966801184 1103 1 0.08882484310726113 1103 2 0.016276740970639025 1103 3 0.04051080788335781 1103 4 0.1531966501273132 1103 5 -0.08069761452873792 1103 6 -0.045035840472998245 1103 7 -0.060810204341905205 1103 8 -0.0022351296901348405 1103 9 0.04173366772813836 1103 10-11 0.07508218369051889 1103 12-13 0.1339433856082266 1103 14-15 -0.1295482949372655 1103 16-17 0.015000452858409119 1103 18-19 -0.10439709355166116 1103 20-21 -0.007043122797568913 1103 22-23 -0.06905853833145414 1103 24-25 0.1754041064591334 1103 26-27 0.2465972657704043 1103 28-29 -0.038990393499162224 1103 30-31 -0.26624550847157025 1103 32-33 -0.1955406438411771 1103 34-35 0.08229356526926068 1103 36-37 -0.3751192834173622 1103 38-39 -0.4258962709443068 1103 40-41 -0.4189292861625731 1103 42-43 -0.1702232867358049 1103 44-45 -0.34821246024790753 1103 46-47 -0.25602975819432316 1103 48-49 -0.23198688835374526 1103 50-51 -0.41184518876105614 1103 52-53 -0.2702813462604965 1103 54-55 -0.2508165750237552 1103 56-57 -0.3621802363528346 1103 58-59 -0.24539305462124616 1103 60-61 0.16495561355589672 1103 62-63 0.1822964302700072 1103 64-65 0.11105444705481915 1103 66-67 0.30153931245213883 1103 68-69 0.31055907439668573 1103 70-71 0.11387795248261057 1103 72-73 -0.22540118175355772 1103 74-75 0.1295752084900883 1103 76-77 0.021168446941597807 1103 78-79 0.16483301356229063 1103 80-81 0.48110131409421086 1103 82-83 0.35706677035127754 1103 84-85 0.5026057768378571 1103 86-87 0.5760507972152986 1103 88-89 0.8530679652371198 1103 90-91 0.7424466478762568 1103 92-93 1.0475884507069217 1103 94-95 1.140635996100233 1103 96-97 1.099802429264411 1103 98-99 1.1323694666818263 1103 100-101 1.7300612148701475 1104 1 -0.0283430766922379 1104 2 -0.024312537102588294 1104 3 0.050841512694162816 1104 4 0.022595757652219106 1104 5 -2.3367936648810428E-5 1104 6 -0.007594676587672211 1104 7 -0.07598533729624535 1104 8 0.07423653728217516 1104 9 -0.15742275992636223 1104 10-11 -0.24497099804430178 1104 12-13 -0.1168665661003061 1104 14-15 -0.080423959708952 1104 16-17 0.007886631720204207 1104 18-19 0.2078469311442248 1104 20-21 -0.10321342660586907 1104 22-23 -0.09397601494983121 1104 24-25 -0.39772841997891106 1104 26-27 0.016349389983375318 1104 28-29 -0.25786838115121213 1104 30-31 -0.2129338757311885 1104 32-33 -0.34529001727475617 1104 34-35 -0.2752237035124523 1104 36-37 -0.3121264354437656 1104 38-39 0.014136738408346616 1104 40-41 -0.05123413364713514 1104 42-43 0.007339522726880432 1104 44-45 -0.12077649227920517 1104 46-47 -0.5113861369460864 1104 48-49 -0.43909765307041937 1104 50-51 -0.5414298210735424 1104 52-53 -0.6730795001818919 1104 54-55 -0.351900076460268 1104 56-57 -0.20071712736083924 1104 58-59 -0.5739660114034493 1104 60-61 -0.11551690921444191 1104 62-63 0.16672396251525612 1104 64-65 -0.3363980255902099 1104 66-67 -0.21775434852958497 1104 68-69 -0.047859838739814364 1104 70-71 0.13174273639360834 1104 72-73 0.21297371891274608 1104 74-75 -0.09973281767288 1104 76-77 0.24852800437175304 1104 78-79 0.03333811778626483 1104 80-81 0.161575365075322 1104 82-83 0.06467420356846176 1104 84-85 0.12900408969316857 1104 86-87 0.15453206135125086 1104 88-89 0.7522366185777862 1104 90-91 1.162269069784685 1104 92-93 0.6897578987171897 1104 94-95 0.5541927538482625 1104 96-97 1.1557120203152103 1104 98-99 1.3380131490585008 1104 100-101 1.9047586291375254 1105 1 -0.15637208292540095 1105 2 -0.007902636269818686 1105 3 0.007592178985248665 1105 4 -0.23600463381636416 1105 5 -0.04910861708257386 1105 6 0.012729910095174546 1105 7 -0.1007518645654244 1105 8 -0.033865128987244475 1105 9 0.02561408290116418 1105 10-11 -0.06992964827217918 1105 12-13 -0.16811139286462407 1105 14-15 0.04310171326310552 1105 16-17 -0.0852930032461785 1105 18-19 0.21405842529748043 1105 20-21 0.03235965595644785 1105 22-23 -0.008326010977363296 1105 24-25 -0.08199672763566213 1105 26-27 -0.35074991447373094 1105 28-29 -0.18655898373837942 1105 30-31 -0.13535881415794648 1105 32-33 -0.19193833836567364 1105 34-35 -0.016670259383396058 1105 36-37 -0.1058542708825101 1105 38-39 0.08557253250453556 1105 40-41 0.10449741424276482 1105 42-43 -0.24963215719338194 1105 44-45 -0.29623675677329686 1105 46-47 0.20055221036310655 1105 48-49 0.6023568477349102 1105 50-51 0.5121695953349104 1105 52-53 0.37988793280981525 1105 54-55 0.05934778382444961 1105 56-57 0.41391744177092704 1105 58-59 -0.007860546010860503 1105 60-61 0.518748870508638 1105 62-63 0.44115283007320016 1105 64-65 0.2531522188970854 1105 66-67 0.7969148894067537 1105 68-69 0.6266687016312886 1105 70-71 0.8929541222068416 1105 72-73 0.58857049182485 1105 74-75 0.9060272597541186 1105 76-77 1.1106725588415038 1105 78-79 0.9838303425845716 1105 80-81 0.8936180077049514 1105 82-83 0.5090957762321118 1105 84-85 0.9741212052486254 1105 86-87 1.5167416198295776 1105 88-89 1.8901570681166184 1105 90-91 1.2462217468664392 1105 92-93 1.1586390352761669 1105 94-95 1.0656406820260536 1105 96-97 1.259718434044057 1105 98-99 1.5045461963741964 1105 100-101 2.227276017709819 1106 1 0.03031420480938607 1106 2 0.11686487451154903 1106 3 -0.16109791033148468 1106 4 -0.1401781985147963 1106 5 -0.08627623414472652 1106 6 -0.1516782898762159 1106 7 -0.1277103946204079 1106 8 -0.19225415753998476 1106 9 -0.2730042668852519 1106 10-11 -0.28783080714151055 1106 12-13 -0.5929637238883956 1106 14-15 -0.41174428179074596 1106 16-17 -0.4495601767904347 1106 18-19 -0.2731827707701271 1106 20-21 -0.10862589394442779 1106 22-23 0.16842200136755991 1106 24-25 0.22109256866290394 1106 26-27 0.10105583695185771 1106 28-29 -0.0028158562218720817 1106 30-31 5.119729330331779E-4 1106 32-33 0.2707714128964156 1106 34-35 -0.492455613073588 1106 36-37 -0.2046902918934066 1106 38-39 0.15030161869416503 1106 40-41 -0.23753765841650676 1106 42-43 -0.22938260173320657 1106 44-45 -0.03652330612232646 1106 46-47 -0.03498236155014922 1106 48-49 0.006791004744329143 1106 50-51 -0.44001938007743746 1106 52-53 -0.6314861551170949 1106 54-55 0.09564165019679649 1106 56-57 -0.678107411582026 1106 58-59 -0.4117786280514508 1106 60-61 -0.4091232655307593 1106 62-63 -0.417902497251184 1106 64-65 -0.3876698221027688 1106 66-67 -0.763133754491296 1106 68-69 -0.5930259454960662 1106 70-71 -0.6933785775294972 1106 72-73 -0.5427986908350313 1106 74-75 -0.4477038089809362 1106 76-77 -0.43500263072662904 1106 78-79 -0.31162002224466434 1106 80-81 -0.1826895525378589 1106 82-83 -0.024484864314008092 1106 84-85 0.056575678239003224 1106 86-87 0.06249345419506014 1106 88-89 0.21342949438432512 1106 90-91 0.5059607457831916 1106 92-93 0.3762080666903138 1106 94-95 -0.0993960052836087 1106 96-97 -0.08292979699401926 1106 98-99 0.5907460451284585 1106 100-101 0.3734836331368854 1107 1 -0.09276719669373534 1107 2 0.20430003911407368 1107 3 0.18761067440441082 1107 4 0.09928935732303756 1107 5 0.24687185519580623 1107 6 0.07233462427642934 1107 7 0.14295754229078028 1107 8 0.14692759328038107 1107 9 0.16335118987350938 1107 10-11 0.1704892944780454 1107 12-13 0.17988744579509586 1107 14-15 0.1641660601167345 1107 16-17 0.17144384565055049 1107 18-19 0.3470802756996463 1107 20-21 0.3458570106813781 1107 22-23 0.19139225647500524 1107 24-25 0.17146041030575532 1107 26-27 0.36284529392233367 1107 28-29 0.3531040125195304 1107 30-31 0.3352743313536948 1107 32-33 0.4033399531737487 1107 34-35 0.4469034427113243 1107 36-37 0.6493771369199735 1107 38-39 0.4491795693663363 1107 40-41 0.4168764412976955 1107 42-43 0.2861381985208382 1107 44-45 0.25100183413383803 1107 46-47 -0.11352581449817478 1107 48-49 0.1574076026060851 1107 50-51 0.4220796989952902 1107 52-53 0.24097335583876145 1107 54-55 0.6717504678108526 1107 56-57 0.7705403072705153 1107 58-59 0.6984547793794817 1107 60-61 1.0156412099641017 1107 62-63 1.0570234051517033 1107 64-65 1.4070136753038156 1107 66-67 1.2846724273653756 1107 68-69 1.3420328033130815 1107 70-71 1.3411561954142925 1107 72-73 0.856415345366937 1107 74-75 0.7427137839056925 1107 76-77 0.9869037945935801 1107 78-79 0.9808140838207535 1107 80-81 1.0379360428892603 1107 82-83 1.1126486172145214 1107 84-85 1.7381809497537652 1107 86-87 1.5180481276363658 1107 88-89 1.6856428954640315 1107 90-91 1.5244057547807586 1107 92-93 1.641675940089275 1107 94-95 1.9872717199201588 1107 96-97 2.6413477514340507 1107 98-99 2.7964727308487944 1107 100-101 2.3302159291038578 1108 1 -0.07108506680264881 1108 2 0.2828743490316654 1108 3 0.10570840966632744 1108 4 -0.04859573459190614 1108 5 0.16180010967629954 1108 6 0.1102675465377061 1108 7 0.022978287983171697 1108 8 -0.028791302587769962 1108 9 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-0.47034660086981717 2316 84-85 -0.3189665650991529 2316 86-87 0.1771736166959208 2316 88-89 0.13369390256445257 2316 90-91 0.8584877718797301 2316 92-93 1.4781670283373565 2316 94-95 1.696806569941895 2316 96-97 2.0197349416963313 2316 98-99 1.8800113484404477 2316 100-101 1.860211581907798 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 5.0 6 14.0 7 70.0 8 150.0 9 259.0 10 457.0 11 724.0 12 1115.0 13 1459.0 14 1940.0 15 2661.0 16 3557.0 17 4886.0 18 4442.0 19 3179.0 20 2750.0 21 2844.0 22 3034.0 23 3264.0 24 3750.0 25 4370.0 26 5214.0 27 6292.0 28 7725.0 29 9454.0 30 11625.0 31 14576.0 32 18043.0 33 23200.0 34 30809.0 35 41892.0 36 56208.0 37 64390.0 38 33283.0 39 1686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.730619719301856 20.91095513759952 16.198520388675636 21.159904754422985 2 16.090715374555614 21.500767604127464 39.661712664249684 22.746804357067237 3 19.477108609891943 25.486893870268133 31.006333072501363 24.02966444733857 4 13.167050694363592 18.268436713950358 37.43850367285714 31.12600891882891 5 12.808564675047588 38.54184999363714 34.447961313835016 14.201624017480254 6 31.077272148831263 37.99762272866345 16.84396923085254 14.081135891652746 7 27.194034619352287 34.24597447817811 20.78650316382865 17.77348773864095 8 26.29348741372915 35.598419845612746 19.283786088901284 18.824306651756824 9 26.20846882606659 17.009674248844533 20.47837847351021 36.30347845157866 10-11 24.42632760315273 27.892188849568544 27.20770799093496 20.473775556343767 12-13 25.68507988085567 25.217979962090443 27.76712699702139 21.329813160032494 14-15 22.345809043919953 26.549220076300124 26.774221497789245 24.330749381990678 16-17 20.81953586349372 29.906235869721197 27.62589655349808 21.648331713286996 18-19 20.89345329799015 27.69466955116142 31.119781442662543 20.29209570818588 20-21 21.47361438280129 26.60290255327214 31.797173259686463 20.126309804240112 22-23 21.432951965526627 26.550888978240238 31.521396263759772 20.494762792473367 24-25 21.39842038713241 26.791685507038405 31.42492777481446 20.384966331014727 26-27 20.749587759224312 27.468773945841445 31.096222006211367 20.68541628872288 28-29 20.619553253392432 27.712559349822442 31.374478933266904 20.29340846351822 30-31 20.945118112687993 27.144054292982283 31.156866700331275 20.75396089399845 32-33 20.820703463979996 27.277133291545898 30.904788271719426 20.99737497275468 34-35 21.183437079476132 27.276480988598305 31.033679815666705 20.506402116258858 36-37 21.722113767866766 26.95441216686387 30.6938762248498 20.62959784041957 38-39 21.102886029063363 27.26253685718231 30.92672968150521 20.70784743224912 40-41 21.304602104886943 27.212310908101404 30.878534431174202 20.604552555837447 42-43 21.147079092702437 27.516250580498763 30.557590215938767 20.779080110860033 44-45 21.021928838900607 27.802973484489524 30.44721401669505 20.72788365991482 46-47 21.7192707896169 27.986277891647333 29.606910873985665 20.687540444750102 48-49 21.45365539312974 28.38212876796153 29.254787710752684 20.909428128156044 50-51 21.478971437545184 28.583303324118475 29.276719257251628 20.661005981084717 52-53 21.786148468447003 28.19598138255386 29.243009658003256 20.77486049099588 54-55 21.5627716059578 28.458212281124517 29.330329704249035 20.648686408668645 56-57 21.644134935870536 28.60103809320095 28.978206541016053 20.776620429912462 58-59 21.258437553305843 28.23808453663516 29.20794625958828 21.295531650470718 60-61 21.110196544563006 28.42842869945929 29.349959792164753 21.11141496381295 62-63 21.273329344138457 28.379015029878346 29.5297443214894 20.8179113044938 64-65 21.00473558948477 28.6103793074505 29.22676406800404 21.158121035060688 66-67 20.854599261908692 28.517779444454973 29.17315362100663 21.4544676726297 68-69 21.23339226872372 28.24282283371826 29.17328900092329 21.350495896634726 70-71 21.362544709217477 28.26326520113394 28.815750641023907 21.558439448624675 72-73 21.247365164804965 27.968716366506424 29.01141389622574 21.772504572462875 74-75 21.07039484906493 28.33718263563037 28.738177948777654 21.854244566527044 76-77 21.069717949481632 28.12802066439048 28.925814513268584 21.876446872859308 78-79 21.087452718564105 28.299547018798854 28.806003287024378 21.806996975612662 80-81 20.996071274818522 28.35748962312939 28.822519636856914 21.82391946519518 82-83 21.04004830381584 28.227407000262637 28.843253576079864 21.88929111984166 84-85 20.906991289656162 28.27220027563351 28.64584884561545 22.17495958909488 86-87 20.943273107321076 28.135195799973467 28.60997316770052 22.31155792500494 88-89 20.66466123883454 27.815563816738916 28.735876490194435 22.783898454232112 90-91 20.572199152708745 28.181302456440594 28.619164379648222 22.62733401120244 92-93 20.45206551375881 28.098541400980704 28.831766970733252 22.617626114527233 94-95 20.19435140835727 28.06249678472698 28.498149356539255 23.245002450376493 96-97 20.34516463551665 28.150629110472718 28.305368355215236 23.19883789879539 98-99 20.25115784143969 28.637977740743697 27.8580508732717 23.25281354454491 100-101 20.48862254693555 28.4677334185435 28.00413233211385 23.039511702407097 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 118.0 2 90.0 3 113.5 4 118.5 5 105.5 6 147.5 7 357.0 8 587.0 9 686.0 10 652.5 11 572.5 12 606.5 13 622.5 14 643.5 15 708.0 16 760.0 17 817.0 18 893.5 19 944.0 20 985.5 21 1160.0 22 1381.0 23 1641.0 24 1966.5 25 2378.5 26 3013.5 27 3800.0 28 4442.5 29 5139.5 30 5898.0 31 6715.5 32 7776.0 33 8480.0 34 8843.5 35 9383.5 36 10269.5 37 11057.0 38 11536.0 39 11676.0 40 11740.0 41 12340.5 42 12496.5 43 12350.5 44 12687.0 45 13067.5 46 13029.0 47 12986.5 48 13458.0 49 13173.0 50 12175.5 51 11501.5 52 10992.0 53 10910.0 54 10989.0 55 9876.5 56 8343.0 57 7489.0 58 7036.0 59 6083.0 60 4600.0 61 3827.5 62 3181.5 63 2180.5 64 1417.0 65 1011.0 66 762.5 67 596.5 68 500.0 69 420.0 70 311.0 71 196.5 72 141.0 73 101.0 74 55.0 75 39.0 76 27.5 77 18.5 78 17.5 79 14.5 80 10.0 81 5.5 82 2.5 83 1.5 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04873676999764439 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.008393554832927645 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.707598333202466E-4 22-23 6.768995833006165E-4 24-25 0.0 26-27 0.002166078666561973 28-29 0.004467537249784069 30-31 4.061397499803699E-4 32-33 4.061397499803699E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009882733916189 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.353799166601233E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.415196666404932E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009882733916189 92-93 0.0010830393332809864 94-95 0.0 96-97 0.0 98-99 4.061397499803699E-4 100-101 0.012996471999371836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 369331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.00661951283321 #Duplication Level Percentage of deduplicated Percentage of total 1 87.71539919964967 63.16089375585449 2 7.629403088461305 10.987350506017355 3 1.9820804054175296 4.281687287902267 4 0.8428145099809357 2.427528949603688 5 0.46541727444172176 1.675656229771247 6 0.28785927331603006 1.2436663900144822 7 0.1992865508408579 1.0044965588295782 8 0.1362539786676206 0.784895071922324 9 0.1082227471341989 0.7013478757973239 >10 0.5774647309874663 7.299856890175568 >50 0.03506240826183404 1.7021536373832895 >100 0.018096726842927856 2.524182538931445 >500 0.001508060570243988 0.6838476893491406 >1k 0.001131045427682991 1.522436618447789 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2254 0.6102926643038359 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1973 0.5342091511408465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1381 0.37391932981526055 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 728 0.19711315865713952 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 658 0.17815997032472225 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 570 0.15433310499254055 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 563 0.15243778615929882 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 459 0.1242787634939932 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 457 0.12373724382735271 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.707598333202466E-4 2 0.0 0.0 0.0 0.0 2.707598333202466E-4 3 0.0 0.0 0.0 0.0 2.707598333202466E-4 4 0.0 0.0 0.0 0.0 2.707598333202466E-4 5 0.0 0.0 0.0 0.0 2.707598333202466E-4 6 0.0 0.0 0.0 0.0 2.707598333202466E-4 7 0.0 0.0 0.0 0.0 2.707598333202466E-4 8 0.0 0.0 0.0 0.0 2.707598333202466E-4 9 0.0 0.0 0.0 0.0 2.707598333202466E-4 10-11 0.0 0.0 0.0 0.0 2.707598333202466E-4 12-13 0.0 0.0 0.0 0.0 2.707598333202466E-4 14-15 0.0 0.0 0.0 0.0 2.707598333202466E-4 16-17 0.0 0.0 0.0 2.707598333202466E-4 2.707598333202466E-4 18-19 0.0 0.0 0.0 2.707598333202466E-4 2.707598333202466E-4 20-21 0.0 0.0 0.0 2.707598333202466E-4 2.707598333202466E-4 22-23 0.0 0.0 0.0 5.415196666404932E-4 2.707598333202466E-4 24-25 0.0 0.0 0.0 8.122794999607399E-4 2.707598333202466E-4 26-27 0.0 0.0 0.0 0.001353799166601233 2.707598333202466E-4 28-29 0.0 0.0 0.0 0.0035198778331632058 2.707598333202466E-4 30-31 0.0 0.0 0.0 0.007987415082947275 2.707598333202466E-4 32-33 0.0 0.0 0.0 0.01841166866577677 2.707598333202466E-4 34-35 0.0 0.0 0.0 0.033303459498390336 2.707598333202466E-4 36-37 0.0 0.0 0.0 0.05266278758078796 2.707598333202466E-4 38-39 0.0 0.0 0.0 0.08515396757921756 2.707598333202466E-4 40-41 0.0 0.0 0.0 0.12590332249391467 2.707598333202466E-4 42-43 0.0 0.0 0.0 0.17815997032472225 2.707598333202466E-4 44-45 0.0 0.0 0.0 0.24747448765470542 2.707598333202466E-4 46-47 0.0 0.0 0.0 0.326130219234237 2.707598333202466E-4 48-49 0.0 0.0 0.0 0.4278005366459896 2.707598333202466E-4 50-51 0.0 0.0 0.0 0.5547868984731853 2.707598333202466E-4 52-53 0.0 0.0 0.0 0.6963942912996742 2.707598333202466E-4 54-55 0.0 0.0 0.0 0.837730924292843 2.707598333202466E-4 56-57 0.0 0.0 0.0 1.0336256637000414 2.707598333202466E-4 58-59 0.0 0.0 0.0 1.2487443512729772 2.707598333202466E-4 60-61 0.0 0.0 0.0 1.4939173803444605 2.707598333202466E-4 62-63 0.0 0.0 0.0 1.7939192756632938 2.707598333202466E-4 64-65 0.0 0.0 0.0 2.0843091968992584 2.707598333202466E-4 66-67 0.0 0.0 0.0 2.4509180112148723 2.707598333202466E-4 68-69 0.0 0.0 0.0 2.891985779693554 2.707598333202466E-4 70-71 0.0 0.0 0.0 3.4255180312510998 2.707598333202466E-4 72-73 0.0 0.0 0.0 4.001288816806604 2.707598333202466E-4 74-75 0.0 0.0 0.0 4.57827802161205 2.707598333202466E-4 76-77 0.0 0.0 0.0 5.239879674330073 2.707598333202466E-4 78-79 0.0 0.0 0.0 6.02711388970869 2.707598333202466E-4 80-81 0.0 0.0 0.0 6.96123531466354 2.707598333202466E-4 82-83 0.0 0.0 0.0 7.850952126953871 2.707598333202466E-4 84-85 0.0 0.0 0.0 8.833268802239726 2.707598333202466E-4 86-87 0.0 0.0 0.0 9.877995619105898 2.707598333202466E-4 88-89 0.0 0.0 0.0 11.06608976771514 2.707598333202466E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1225 0.0 44.9937 1 GTATCAA 2055 0.0 36.30092 1 GTGTAAG 240 0.0 29.696812 1 ATCAACG 2540 0.0 29.170527 3 TATCAAC 2565 0.0 29.07138 2 TCAACGC 2550 0.0 29.056131 4 ACCGAGT 115 3.8498874E-6 28.918194 8 CAACGCA 2605 0.0 28.442663 5 AACGCAG 2650 0.0 28.1389 6 GTATAGG 155 4.555841E-8 27.589294 1 TATAGGG 155 4.573849E-8 27.57809 2 TGTCGGA 35 0.008390685 27.14218 66-67 TAAGGTG 240 9.094947E-12 25.726784 5 TAAGGTA 150 1.057706E-6 25.330986 4 CGCAGAG 2950 0.0 25.123121 8 ACGCAGA 2960 0.0 25.038246 7 TGTAAGG 230 1.3460522E-10 24.780312 2 GTACAGG 520 0.0 24.671196 1 GTCTAGA 175 1.4759644E-7 24.436234 1 GTAAGGC 145 2.3304656E-5 22.92891 3 >>END_MODULE