FastQCFastQC Report
Thu 26 May 2016
SRR937166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325270
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21880.6726719340855289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17310.5321732714360378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.35601192855166475No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4820.14818458511390536No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.13619454606941925No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA3260.1002244289359609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8700.047.010261
GTATCAA14750.040.6248741
ATAGCCC1203.765308E-935.6346633
ACACCGT702.0486866E-433.9325376
CCCTTAT753.0520992E-431.7045961
GCTAGAC600.003941570431.6752553
CTAGTAC753.068726E-431.6752533
GCGACAG1351.1885277E-831.6752517
CGACAGC1351.1885277E-831.6752518
TATCAAC19100.031.5972082
GTATAGC1551.3315002E-930.6818681
ATCAACG19950.030.4844533
TCAACGC20150.030.200514
CAACGCA20650.029.4647185
AACGCAG21300.028.7709676
AGGCGAC1554.5336492E-827.6008955
GGCGACA1554.560934E-827.583876
CACGTAT350.00839676327.13765720-21
CGGTGCA350.00840308127.13347430-31
GTATAGG1109.379394E-525.9401251
Produced by FastQC (version 0.11.5)