##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937166_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 325270 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926534878716144 33.0 31.0 34.0 31.0 34.0 2 32.04597411381314 33.0 31.0 34.0 30.0 34.0 3 32.07807052602453 34.0 31.0 34.0 30.0 34.0 4 35.67723122329142 37.0 35.0 37.0 35.0 37.0 5 35.68504319488425 37.0 35.0 37.0 35.0 37.0 6 35.73837119931134 37.0 35.0 37.0 35.0 37.0 7 35.68961785593507 37.0 35.0 37.0 35.0 37.0 8 35.69374673348295 37.0 35.0 37.0 35.0 37.0 9 37.36767301011467 39.0 37.0 39.0 35.0 39.0 10-11 37.26304147323762 39.0 37.0 39.0 35.0 39.0 12-13 37.137708672794915 39.0 37.0 39.0 34.0 39.0 14-15 38.36780674516555 40.0 38.0 41.0 34.0 41.0 16-17 38.33888154456298 40.0 38.0 41.0 34.0 41.0 18-19 38.347113167522366 40.0 38.0 41.0 34.0 41.0 20-21 38.300880806714424 40.0 38.0 41.0 34.0 41.0 22-23 38.16938389645525 40.0 38.0 41.0 34.0 41.0 24-25 38.02492698373659 40.0 38.0 41.0 34.0 41.0 26-27 37.87174654902081 40.0 38.0 41.0 33.0 41.0 28-29 37.889600946905645 40.0 38.0 41.0 33.0 41.0 30-31 37.690240415654685 40.0 37.5 41.0 33.0 41.0 32-33 37.44960494358533 40.0 37.0 41.0 33.0 41.0 34-35 37.30802564023735 40.0 37.0 41.0 33.0 41.0 36-37 37.31425123743352 40.0 37.0 41.0 33.0 41.0 38-39 37.209324561133826 40.0 37.0 41.0 32.0 41.0 40-41 37.029297199249854 40.0 36.5 41.0 31.5 41.0 42-43 36.76736249884711 40.0 36.0 41.0 31.0 41.0 44-45 36.50063639438005 40.0 36.0 41.0 30.5 41.0 46-47 36.11210379069696 40.0 36.0 41.0 29.5 41.0 48-49 35.721754234943276 39.0 35.5 41.0 27.5 41.0 50-51 34.802451809266145 38.5 34.0 40.0 25.5 40.5 52-53 34.850059950195224 38.5 35.0 40.0 26.0 40.5 54-55 35.15809020198604 39.0 35.0 40.5 26.0 41.0 56-57 34.96957604451686 39.0 35.0 40.5 25.5 41.0 58-59 34.73420696652012 39.0 35.0 40.0 24.0 41.0 60-61 34.463816214222035 38.0 34.5 40.0 23.0 41.0 62-63 34.14208964859962 38.0 34.0 40.0 22.5 41.0 64-65 33.77453346450641 37.5 34.0 40.0 21.5 41.0 66-67 33.39920681280167 37.0 34.0 40.0 19.5 41.0 68-69 32.94607864235866 36.0 33.0 39.0 17.5 41.0 70-71 32.50739232022627 36.0 33.0 39.0 13.5 40.5 72-73 32.10758139391889 35.0 32.0 38.5 8.0 40.0 74-75 31.679621545177852 35.0 32.0 37.5 7.0 40.0 76-77 31.263673256064195 35.0 31.5 37.0 2.0 39.0 78-79 30.804387124542686 35.0 31.0 36.5 2.0 39.0 80-81 30.34592646109386 35.0 31.0 36.0 2.0 38.5 82-83 29.883834967872843 34.0 30.0 36.0 2.0 37.0 84-85 29.456385464383438 34.0 30.0 35.0 2.0 37.0 86-87 29.03852491776063 34.0 29.0 35.0 2.0 36.5 88-89 28.63618839733145 34.0 29.0 35.0 2.0 36.0 90-91 28.239450302825347 34.0 29.0 35.0 2.0 36.0 92-93 27.820130968118793 34.0 28.0 35.0 2.0 35.0 94-95 27.318613767024317 34.0 26.5 35.0 2.0 35.0 96-97 26.752940633934884 33.5 25.5 35.0 2.0 35.0 98-99 26.04081839702401 33.0 24.5 35.0 2.0 35.0 100-101 24.220598272204633 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0624144278980765 1101 2 0.0375350800436145 1101 3 0.14213179093253103 1101 4 0.16549470797436783 1101 5 0.17787438222705276 1101 6 0.13514728826706346 1101 7 0.14714379725553073 1101 8 0.18477391651169484 1101 9 0.1453953692971055 1101 10-11 0.03271085083724756 1101 12-13 -0.07619130010107256 1101 14-15 0.0863269635415449 1101 16-17 0.2683420226357285 1101 18-19 0.24966434490814038 1101 20-21 0.22869660834079042 1101 22-23 0.2567190515831186 1101 24-25 0.0856570468859843 1101 26-27 -0.002732134273962572 1101 28-29 0.07015628004327112 1101 30-31 0.20337901736620978 1101 32-33 0.4344730362457341 1101 34-35 0.30509743688464397 1101 36-37 0.3825745743892526 1101 38-39 0.34301700933323076 1101 40-41 0.4024912924645534 1101 42-43 0.3253082196410304 1101 44-45 0.5413699255867854 1101 46-47 0.47045210603148746 1101 48-49 0.4099448101177501 1101 50-51 0.39298462261999845 1101 52-53 0.483110917842275 1101 54-55 0.46521377773182593 1101 56-57 0.5347766951278459 1101 58-59 0.6228983904161751 1101 60-61 0.43776634707234763 1101 62-63 0.5776025509474891 1101 64-65 0.3700548872381333 1101 66-67 0.3453132833744519 1101 68-69 0.5295196057009122 1101 70-71 0.5627763851791343 1101 72-73 0.5789250949027718 1101 74-75 0.6598523596612829 1101 76-77 0.6346355503552843 1101 78-79 0.840401545223397 1101 80-81 0.7305952256789112 1101 82-83 0.7893097054952882 1101 84-85 0.8803140834172076 1101 86-87 0.9448427945153419 1101 88-89 0.9977687481493156 1101 90-91 0.9966389417761903 1101 92-93 1.1145652778643687 1101 94-95 0.9789334732770882 1101 96-97 0.8286815845216147 1101 98-99 1.2769758304743952 1101 100-101 1.821398432933961 1102 1 -0.1714936484275995 1102 2 0.06652412356905657 1102 3 -0.011400884563173008 1102 4 -0.08005450875697306 1102 5 0.0826002154344394 1102 6 -0.013851296878236496 1102 7 0.052193754190774655 1102 8 -0.017985505382675626 1102 9 0.08223858627972902 1102 10-11 0.14245313605520238 1102 12-13 0.11031459148500744 1102 14-15 0.16153368346404307 1102 16-17 0.18930408741314153 1102 18-19 0.1680051956054598 1102 20-21 0.332821355915776 1102 22-23 0.2766062273911061 1102 24-25 0.15822798536984806 1102 26-27 0.012325962172873517 1102 28-29 0.22505009310399515 1102 30-31 0.019986898671369602 1102 32-33 0.18283375317828643 1102 34-35 0.18533280898050464 1102 36-37 0.2190942139199592 1102 38-39 0.3043789116845801 1102 40-41 0.5150978845584788 1102 42-43 0.3920294532049553 1102 44-45 0.48876719384286815 1102 46-47 -0.013264318483187765 1102 48-49 0.15913495602805483 1102 50-51 0.4488231288830491 1102 52-53 0.4117654583968928 1102 54-55 0.5414638847108719 1102 56-57 0.44940833215297715 1102 58-59 0.4837755639115997 1102 60-61 0.3431631892332021 1102 62-63 0.17521295786843893 1102 64-65 0.20013757520714393 1102 66-67 0.3256091100083225 1102 68-69 0.22024183712889567 1102 70-71 0.2907491733533547 1102 72-73 0.2846671834613872 1102 74-75 0.39071221620922003 1102 76-77 0.1888725576371435 1102 78-79 0.21089317536544883 1102 80-81 0.4334884851800176 1102 82-83 0.2180134108850318 1102 84-85 -0.019183888988571596 1102 86-87 -0.047103277123284215 1102 88-89 0.02907491214499558 1102 90-91 0.06744722987484408 1102 92-93 -0.062124034975123976 1102 94-95 0.04718427948933979 1102 96-97 0.3286542707243214 1102 98-99 0.3579357465664117 1102 100-101 0.7635723926879869 1103 1 -0.20133085463307054 1103 2 -0.43048522015111956 1103 3 -0.1951930811142084 1103 4 -0.18320163351005903 1103 5 -0.28167170679739684 1103 6 -0.20200741024574853 1103 7 -0.20506371967565684 1103 8 -0.19336890862745548 1103 9 -0.04207339632009166 1103 10-11 -0.12496785291432388 1103 12-13 -0.12646465480302993 1103 14-15 -0.18114059578223873 1103 16-17 -0.26095475139104707 1103 18-19 -0.16323911715393535 1103 20-21 -0.3005624690170876 1103 22-23 -0.37082924323498645 1103 24-25 -0.21518929718336466 1103 26-27 -0.09675947405235519 1103 28-29 -0.17144490212427144 1103 30-31 -0.2194278981415536 1103 32-33 -0.016431899217572266 1103 34-35 0.21576925614742493 1103 36-37 -0.06574221549166737 1103 38-39 -0.06477155328202144 1103 40-41 0.09116958262981711 1103 42-43 -0.1037897313834577 1103 44-45 0.13914754539757013 1103 46-47 0.17595711214912058 1103 48-49 0.407997507906785 1103 50-51 0.09933941935955914 1103 52-53 0.361569317952565 1103 54-55 0.4575548916576295 1103 56-57 0.490908542997019 1103 58-59 0.3473400488972729 1103 60-61 0.4720929337789954 1103 62-63 0.36232872234454305 1103 64-65 0.29259684901587235 1103 66-67 0.3452023474631005 1103 68-69 0.414165644750355 1103 70-71 0.5427702244663877 1103 72-73 0.5255315330630026 1103 74-75 0.6114132171635696 1103 76-77 0.6506695592129255 1103 78-79 0.5675067426246976 1103 80-81 0.7072556023871073 1103 82-83 0.31930871461142374 1103 84-85 0.43284493006285274 1103 86-87 0.5972375023338472 1103 88-89 0.7784839939746782 1103 90-91 0.6630428088081928 1103 92-93 0.6054179118921788 1103 94-95 0.5820650971203385 1103 96-97 1.0847701350739243 1103 98-99 0.9464708243567657 1103 100-101 0.7356535898382681 1104 1 0.20233517307451976 1104 2 0.13494273721288508 1104 3 0.11556974889363403 1104 4 0.04611206635343024 1104 5 0.2061735489314529 1104 6 0.04109473607587688 1104 7 0.10679913647141603 1104 8 0.13606532215294465 1104 9 0.03939025562147691 1104 10-11 0.19097744347556045 1104 12-13 -0.05163564896526651 1104 14-15 0.2182995889350181 1104 16-17 0.22084036057200507 1104 18-19 0.30186863458141744 1104 20-21 0.2915964865752585 1104 22-23 0.23966711179391353 1104 24-25 0.1860171011204912 1104 26-27 0.09542017510102596 1104 28-29 0.45960342641686935 1104 30-31 0.33883899796951056 1104 32-33 0.34823039638813214 1104 34-35 0.4630137451253802 1104 36-37 0.213689837019146 1104 38-39 0.23319212770314124 1104 40-41 0.13151295052502832 1104 42-43 0.5975077911979412 1104 44-45 0.5054943694805161 1104 46-47 0.5824585207634527 1104 48-49 0.5567878421985597 1104 50-51 0.3512435900197133 1104 52-53 0.4266650892052226 1104 54-55 0.6799798139660069 1104 56-57 0.6332185252804265 1104 58-59 0.6413955512062444 1104 60-61 0.5623949145521365 1104 62-63 0.2450325357684946 1104 64-65 0.4200383261730778 1104 66-67 0.2769589766635292 1104 68-69 0.2555912495832118 1104 70-71 0.6275575527461399 1104 72-73 0.6999709916904067 1104 74-75 0.6161799971002502 1104 76-77 0.8247768317289719 1104 78-79 0.9850356410011614 1104 80-81 0.8666795226914843 1104 82-83 0.9949516671832335 1104 84-85 0.635512947256661 1104 86-87 0.7311883750938577 1104 88-89 0.7509397751021503 1104 90-91 1.18841964944599 1104 92-93 1.2408463775190874 1104 94-95 1.2099186302736022 1104 96-97 1.0130705765365597 1104 98-99 1.0988371512133739 1104 100-101 1.659169099117694 1105 1 0.06298658342209507 1105 2 -0.07459461706556425 1105 3 0.013607760400518032 1105 4 -0.07415535020010111 1105 5 0.03179252882672756 1105 6 -0.07204013581844748 1105 7 0.12616582684140099 1105 8 0.1878840132986781 1105 9 0.17338943677948748 1105 10-11 0.15276122609455456 1105 12-13 0.017790838418108024 1105 14-15 -0.014006420934180142 1105 16-17 0.2622161228492601 1105 18-19 0.21252058364082416 1105 20-21 0.17436740475570645 1105 22-23 0.23219787223953148 1105 24-25 0.10023262895034435 1105 26-27 0.12504393875737208 1105 28-29 0.2656752474783488 1105 30-31 0.3278196474404851 1105 32-33 0.014277544447693913 1105 34-35 0.030285385179681157 1105 36-37 0.16643403509272048 1105 38-39 0.28933667486197834 1105 40-41 0.26230679628105236 1105 42-43 0.4027553831560482 1105 44-45 0.30438246825871573 1105 46-47 0.41672382556660637 1105 48-49 0.5235304672335701 1105 50-51 0.7930923412856927 1105 52-53 0.9295041005612674 1105 54-55 0.5968431104514522 1105 56-57 0.8694732928801443 1105 58-59 0.796673849738788 1105 60-61 0.5634615639800202 1105 62-63 0.8490631776975164 1105 64-65 0.4785774474263391 1105 66-67 0.3628215620493549 1105 68-69 -0.07514010560026918 1105 70-71 0.11730856013726765 1105 72-73 0.4906437350265378 1105 74-75 0.3023318707400051 1105 76-77 0.1759442202694963 1105 78-79 0.12182534945031875 1105 80-81 0.33377500397663695 1105 82-83 0.3105721554716361 1105 84-85 0.48301440177860755 1105 86-87 0.48278565567652976 1105 88-89 0.18246950122105687 1105 90-91 0.7154037251242258 1105 92-93 0.6365948637388392 1105 94-95 0.7757303612624575 1105 96-97 0.9589812150178219 1105 98-99 1.127747121691968 1105 100-101 1.2864074467807605 1106 1 0.002065884497362447 1106 2 0.15067420013873445 1106 3 0.2451601797553593 1106 4 0.16539438098268988 1106 5 0.34862720624607846 1106 6 0.37349077816004694 1106 7 -0.06634089358870199 1106 8 -0.056638836163692474 1106 9 0.2595117486074443 1106 10-11 -0.13626767175490784 1106 12-13 -0.03414128523780846 1106 14-15 -0.23609983491267883 1106 16-17 -0.2654384470432163 1106 18-19 0.12668389009243697 1106 20-21 0.02833245851914512 1106 22-23 -0.021599170771217757 1106 24-25 0.19665399991809096 1106 26-27 -0.17737541608133967 1106 28-29 -0.009375155747456176 1106 30-31 0.0813646743222094 1106 32-33 0.333817194985329 1106 34-35 0.15326925614742493 1106 36-37 0.19591992218948917 1106 38-39 0.2709394974426189 1106 40-41 0.4642221188616986 1106 42-43 0.19917675412379765 1106 44-45 0.07630787148452356 1106 46-47 0.22715729330854373 1106 48-49 -0.10941644136858031 1106 50-51 0.19856949182332784 1106 52-53 0.05814993389459744 1106 54-55 0.260838406150377 1106 56-57 0.09013861546078772 1106 58-59 0.3681960271581417 1106 60-61 0.44541720914131844 1106 62-63 0.30102890350396194 1106 64-65 0.5148341678564492 1106 66-67 0.7567059706515096 1106 68-69 0.6467516954749897 1106 70-71 0.5144187751910252 1106 72-73 0.5611746490050322 1106 74-75 0.3757021664389306 1106 76-77 0.5892338708071314 1106 78-79 0.585283010740639 1106 80-81 0.6261641168798597 1106 82-83 0.4620170479447552 1106 84-85 0.048084966294734244 1106 86-87 0.03029909653674423 1106 88-89 -0.16492633211228025 1106 90-91 0.237295526199496 1106 92-93 0.26268693363131135 1106 94-95 0.04762216233772776 1106 96-97 0.5775464031898672 1106 98-99 0.9796456431973439 1106 100-101 0.7635747854904409 1107 1 0.14714003703973688 1107 2 0.15321657302009228 1107 3 0.22074280687400005 1107 4 0.16798971996573897 1107 5 -0.03627745477086819 1107 6 0.16602255782106567 1107 7 0.1831824114960412 1107 8 0.13292684180240855 1107 9 0.12704352826846588 1107 10-11 0.04140711638069661 1107 12-13 0.1566690961181223 1107 14-15 0.24242241085003968 1107 16-17 0.03334333261779676 1107 18-19 -0.00840615228044328 1107 20-21 0.20153161106151884 1107 22-23 -0.0013131538220676475 1107 24-25 0.14419107618927995 1107 26-27 -0.09898558557286918 1107 28-29 0.08058776798134915 1107 30-31 0.3286898861866163 1107 32-33 0.5203373221039698 1107 34-35 0.5611082391982762 1107 36-37 0.5074400493081299 1107 38-39 0.5624914042222784 1107 40-41 0.3769869493701705 1107 42-43 0.6734087456492119 1107 44-45 0.5187072358913056 1107 46-47 0.8472049628000704 1107 48-49 1.115980804394141 1107 50-51 0.8597453392809555 1107 52-53 1.0230069254200131 1107 54-55 1.070822516319872 1107 56-57 1.1871195476641745 1107 58-59 1.2302987813954331 1107 60-61 1.2860422091413213 1107 62-63 1.301796911978542 1107 64-65 1.2863913712462747 1107 66-67 1.0813881740413365 1107 68-69 0.8496913141190561 1107 70-71 0.8670670802757741 1107 72-73 0.6965030388355444 1107 74-75 0.7004638189813015 1107 76-77 0.7519192521630593 1107 78-79 0.79399593446945 1107 80-81 1.055480854167996 1107 82-83 1.1003115394701801 1107 84-85 1.3067978900235495 1107 86-87 1.264091469418105 1107 88-89 1.4152378628029751 1107 90-91 1.4080317550130559 1107 92-93 1.2650439251567356 1107 94-95 1.234883814880103 1107 96-97 1.2125305133593578 1107 98-99 1.5002229737058173 1107 100-101 1.676101268541288 1108 1 -0.02241955726970346 1108 2 -0.10775953480102274 1108 3 -0.2957911254655272 1108 4 -0.1834635507442144 1108 5 -0.015039962428616604 1108 6 0.0365555371961932 1108 7 0.06414354416229884 1108 8 -0.1756021895773472 1108 9 -0.3092508015933575 1108 10-11 -0.14857314364245156 1108 12-13 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-3.343533829279721 2316 100-101 -4.181852393408644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1678.0 3 360.0 4 239.0 5 275.0 6 296.0 7 320.0 8 337.0 9 451.0 10 562.0 11 756.0 12 1049.0 13 1365.0 14 1637.0 15 2134.0 16 2991.0 17 3984.0 18 3371.0 19 2188.0 20 1948.0 21 2018.0 22 2013.0 23 2184.0 24 2387.0 25 2594.0 26 2979.0 27 3373.0 28 4046.0 29 4972.0 30 6114.0 31 7688.0 32 10021.0 33 13576.0 34 19474.0 35 29320.0 36 44735.0 37 68092.0 38 62747.0 39 10992.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70025819865564 19.521299075482233 15.737470270136871 22.040972455725257 2 14.87854063754602 19.991921210215043 41.6865552903739 23.442982861865026 3 18.623445579479338 25.031373625187705 30.985733094472383 25.359447700860578 4 12.102346835887996 16.806502440573652 38.66960213870105 32.4215485848373 5 12.630702579362021 37.53565316600114 35.39476727154993 14.43887698308691 6 30.238118714257546 37.278458390849934 18.014814632222443 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26.985288454132327 31.975198336285914 20.78980204883309 30-31 20.37655272924469 26.68021364999792 31.79622831031938 21.147005310438008 32-33 20.063190909875157 26.714257545321225 31.75135255043565 21.471198994367967 34-35 20.888674568118827 26.77701789766731 31.494163268261644 20.84014426595222 36-37 20.893271658279534 26.478838093741768 31.50480045717173 21.12308979080697 38-39 20.36024435250591 26.880007886313855 31.473939737472005 21.28580802370823 40-41 20.889963649805924 26.823670753496398 31.437064875854848 20.84930072084283 42-43 20.43949882469647 26.960507952938567 31.151088888683503 21.448904333681458 44-45 20.52291226362197 27.226990689678544 31.206059411065173 21.044037635634318 46-47 20.917303147847466 27.565771433677067 30.307082271675533 21.20984314679993 48-49 20.662838902693967 27.693466663174743 30.20549454104576 21.43819989308553 50-51 20.992354063992927 27.851666068405166 29.98137429652921 21.174605571072696 52-53 21.14327976456449 27.75550453768047 29.894762792560975 21.206452905194066 54-55 20.705910626556918 27.798754927832952 30.13253347999365 21.362800965616476 56-57 20.753635956191406 27.945305485007214 29.85927467236735 21.44178388643403 58-59 20.781204998159495 27.638349615496573 29.88376337445074 21.69668201189319 60-61 20.476394360644022 27.631088536163894 30.241770292040275 21.650746811151805 62-63 20.604227187450903 27.474253957789735 30.581184364150545 21.340334490608818 64-65 20.521859930724652 27.51870111028457 30.35008848085533 21.60935047813545 66-67 20.276786553089515 27.55128913968542 30.19240454082725 21.979519766397818 68-69 20.749733569065672 27.179805459216045 30.295045308659468 21.775415663058812 70-71 20.57370282292347 27.2870793646882 30.060407605012507 22.078810207375827 72-73 20.39803217280406 27.131556086771692 30.10332016930461 22.36709157111964 74-75 20.491401778177583 27.47580113492831 29.872428389227423 22.16036869766668 76-77 20.573366727355065 27.050389853639302 30.112227325798113 22.26401609320752 78-79 20.59174623516129 27.093438236929536 30.027986956876674 22.286828571032498 80-81 20.53427436434967 27.03857107869942 30.029250620362685 22.39790393658823 82-83 20.535990697172956 27.031027392513074 30.010396371281374 22.4225855390326 84-85 20.32781633907107 27.0717219521135 29.86892582627859 22.73153588253684 86-87 20.552065509843572 27.087186800696745 29.771895266799834 22.58885242265985 88-89 20.471718164260434 26.8955437203328 29.59681865926572 23.035919456141055 90-91 20.193790151541986 26.821037521235745 30.02622990208838 22.958942425133884 92-93 20.31856433508705 26.73975185125861 29.992545680807286 22.949138132847057 94-95 20.115694002300945 26.672611539521853 29.868211717560015 23.343482740617187 96-97 20.255535157645728 26.75264591008218 29.561653709202467 23.430165223069626 98-99 20.487181264391715 27.353156358539533 28.950704279586287 23.20895809748246 100-101 20.866905677114133 27.220702739996057 28.59070076877587 23.32169081411394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 484.0 1 291.5 2 111.5 3 130.0 4 105.5 5 88.0 6 156.5 7 355.0 8 628.5 9 759.0 10 673.0 11 560.0 12 540.0 13 557.5 14 568.0 15 608.0 16 689.5 17 729.0 18 763.0 19 848.0 20 946.0 21 1103.5 22 1265.0 23 1472.5 24 1748.5 25 2111.0 26 2789.5 27 3540.5 28 4005.5 29 4477.0 30 5089.5 31 5931.0 32 6928.0 33 7438.5 34 7743.0 35 8298.0 36 9033.0 37 9555.5 38 9797.5 39 10084.0 40 10322.0 41 10694.5 42 10832.5 43 10886.0 44 11132.0 45 11279.5 46 11421.5 47 11638.0 48 11940.5 49 11631.0 50 10846.5 51 10342.0 52 9851.0 53 9496.5 54 9304.5 55 8444.0 56 7413.0 57 6591.0 58 5902.0 59 5155.0 60 4105.0 61 3346.0 62 2775.0 63 2010.5 64 1329.0 65 951.5 66 728.5 67 575.0 68 444.5 69 327.5 70 232.0 71 158.0 72 100.0 73 74.0 74 62.5 75 44.0 76 33.5 77 26.5 78 16.0 79 11.0 80 11.0 81 7.5 82 1.0 83 1.5 84 1.5 85 0.5 86 0.5 87 0.0 88 1.5 89 1.5 90 1.0 91 1.0 92 1.0 93 1.5 94 0.5 95 0.5 96 1.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3384880253328004 2 0.29636917022781073 3 0.2929873643434685 4 0.29544685953208105 5 0.2966766071263873 6 0.22319918836658775 7 0.2115165862206782 8 0.21674301349647987 9 0.2087496541334891 10-11 0.23149998462815508 12-13 0.2216620038737049 14-15 0.21551326590217357 16-17 0.21551326590217357 18-19 0.21044055707566023 20-21 0.20490669290128202 22-23 0.2052141297998586 24-25 0.19629845974113813 26-27 0.19860423648046238 28-29 0.21366864451071418 30-31 0.22150828542441664 32-33 0.2145909552064439 34-35 0.22458265441018233 36-37 0.20459925600270545 38-39 0.20198604236480464 40-41 0.19983398407476863 42-43 0.20690503274202968 44-45 0.21028683862637193 46-47 0.21489839210502046 48-49 0.21874135333722752 50-51 0.22089341162726353 52-53 0.2351892274110739 54-55 0.21843391643865095 56-57 0.19383896455252558 58-59 0.19383896455252558 60-61 0.19153318781320133 62-63 0.20198604236480464 64-65 0.19168690626248963 66-67 0.18922741107387708 68-69 0.18692163433455283 70-71 0.1973744888861561 72-73 0.19921911027761552 74-75 0.2053678482491469 76-77 0.20321578995911088 78-79 0.20121745011836323 80-81 0.20336950840839918 82-83 0.19599102284256156 84-85 0.18646047898668797 86-87 0.18953484797245365 88-89 0.1927629354075076 90-91 0.19691333353829124 92-93 0.19245549850893104 94-95 0.19076459556675993 96-97 0.1899960033203185 98-99 0.19906539182832725 100-101 0.18384726534878715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 325270.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.45562600714575 #Duplication Level Percentage of deduplicated Percentage of total 1 88.9316697935878 66.21463146342353 2 6.964740914994673 10.37128289607019 3 1.691158031525101 3.777486897426411 4 0.7531995915120032 2.2431978837741067 5 0.40621802364427084 1.512260862290986 6 0.2730179127516379 1.2196631763052497 7 0.1802573480395063 0.9394821583468571 8 0.13845492240127036 0.8246998336925899 9 0.10753762188199993 0.7206102860889636 >10 0.5054867129170061 6.51361319226529 >50 0.024748270125567504 1.2376452029185343 >100 0.022273443112939988 2.8662388781165173 >500 0.0 0.0 >1k 0.0012374135062744437 1.559187269280784 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2188 0.6726719340855289 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1731 0.5321732714360378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1158 0.35601192855166475 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 482 0.14818458511390536 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 443 0.13619454606941925 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 326 0.1002244289359609 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.537184492882836E-4 0.0 18-19 0.0 0.0 0.0 3.074368985765672E-4 0.0 20-21 0.0 0.0 0.0 3.074368985765672E-4 0.0 22-23 0.0 0.0 0.0 6.148737971531343E-4 0.0 24-25 0.0 0.0 0.0 6.148737971531343E-4 0.0 26-27 0.0 0.0 0.0 0.001076029145017985 0.0 28-29 0.0 0.0 0.0 0.003689242782918806 0.0 30-31 0.0 0.0 0.0 0.00860823316014388 0.0 32-33 0.0 0.0 0.0 0.01752390321886433 0.0 34-35 0.0 0.0 0.0 0.030897408306945 0.0 36-37 0.0 0.0 0.0 0.04949734067082731 0.0 38-39 0.0 0.0 0.0 0.08961785593506932 0.0 40-41 0.0 0.0 0.0 0.13265902173578872 0.0 42-43 0.0 0.0 0.0 0.19184062471177793 0.0 44-45 0.0 0.0 0.0 0.26916100470378457 0.0 46-47 0.0 0.0 0.0 0.3630829772189258 0.0 48-49 0.0 0.0 0.0 0.46469087219848126 0.0 50-51 0.0 0.0 0.0 0.5981184861807114 0.0 52-53 0.0 0.0 0.0 0.7427675469609862 0.0 54-55 0.0 0.0 0.0 0.8971008700464229 0.0 56-57 0.0 0.0 0.0 1.1318289421096321 0.0 58-59 0.0 0.0 0.0 1.37470409198512 0.0 60-61 0.0 0.0 0.0 1.6383312325145265 0.0 62-63 0.0 0.0 0.0 1.9491499369754357 0.0 64-65 0.0 0.0 0.0 2.260276078334922 0.0 66-67 0.0 0.0 0.0 2.647492852092108 0.0 68-69 0.0 0.0 0.0 3.130168782857319 0.0 70-71 0.0 0.0 0.0 3.7058443754419406 0.0 72-73 0.0 0.0 0.0 4.351461862452732 0.0 74-75 0.0 0.0 0.0 4.986165339564055 0.0 76-77 0.0 0.0 0.0 5.692655332493006 0.0 78-79 0.0 0.0 0.0 6.562548037015403 0.0 80-81 0.0 0.0 0.0 7.546346112460418 3.074368985765672E-4 82-83 0.0 0.0 0.0 8.486180711408984 3.074368985765672E-4 84-85 0.0 0.0 0.0 9.558059458296185 3.074368985765672E-4 86-87 0.0 0.0 0.0 10.742152673163833 3.074368985765672E-4 88-89 0.0 0.0 0.0 12.074584191594674 3.074368985765672E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 870 0.0 47.01026 1 GTATCAA 1475 0.0 40.624874 1 ATAGCCC 120 3.765308E-9 35.634663 3 ACACCGT 70 2.0486866E-4 33.932537 6 CCCTTAT 75 3.0520992E-4 31.704596 1 GCTAGAC 60 0.0039415704 31.675255 3 CTAGTAC 75 3.068726E-4 31.675253 3 GCGACAG 135 1.1885277E-8 31.675251 7 CGACAGC 135 1.1885277E-8 31.675251 8 TATCAAC 1910 0.0 31.597208 2 GTATAGC 155 1.3315002E-9 30.681868 1 ATCAACG 1995 0.0 30.484453 3 TCAACGC 2015 0.0 30.20051 4 CAACGCA 2065 0.0 29.464718 5 AACGCAG 2130 0.0 28.770967 6 AGGCGAC 155 4.5336492E-8 27.600895 5 GGCGACA 155 4.560934E-8 27.58387 6 CACGTAT 35 0.008396763 27.137657 20-21 CGGTGCA 35 0.008403081 27.133474 30-31 GTATAGG 110 9.379394E-5 25.940125 1 >>END_MODULE