FastQCFastQC Report
Thu 26 May 2016
SRR937166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325270
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.608725059181603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.5509269222492084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.3584714237402773No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC6720.20659759584345314No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA6190.1903034402188951No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA4770.14664740062102255No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.1386540412580318No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA3700.11375165247332986No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA3450.10606573000891567No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG352.7832057E-454.2926565
GGTATCA11400.047.1183361
GTATCAA19050.037.4293751
GTAAGGT1800.036.949174
GGTAAGG2000.035.629563
AGGTAAG2050.032.4431722
ACCGAGT753.074099E-431.6658468
GTATAGG953.465604E-530.0222991
ATCAACG24050.029.6295683
TATCAAC24100.029.5680942
TAAGGTG2750.029.3673885
TCAACGC24350.029.264524
GTACCTA650.005834346629.2345075
CAACGCA24600.028.967125
AACGCAG25100.028.5749576
GTAGATC1503.317109E-828.5036433
CTTATAG856.392446E-427.944753
AAAGGCG856.392446E-427.944755
GGTGTGC2750.027.6356478
ACGCAGA27650.025.5960837