##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937166_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 325270 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.845998708765027 31.0 31.0 34.0 30.0 34.0 2 32.10167553109724 33.0 31.0 34.0 30.0 34.0 3 32.16072493620684 33.0 31.0 34.0 30.0 34.0 4 35.87946628954407 37.0 35.0 37.0 35.0 37.0 5 35.71003166600055 37.0 35.0 37.0 33.0 37.0 6 35.8044486119224 37.0 35.0 37.0 35.0 37.0 7 35.762809358379194 37.0 35.0 37.0 33.0 37.0 8 35.7463061456636 37.0 35.0 37.0 33.0 37.0 9 37.38569188674025 39.0 37.0 39.0 34.0 39.0 10-11 37.2882712823193 39.0 37.0 39.0 34.0 39.0 12-13 37.187561103083596 39.0 37.0 39.0 33.0 39.0 14-15 38.27676853075906 40.0 38.0 41.0 33.0 41.0 16-17 38.237960771051746 40.0 38.0 41.0 33.0 41.0 18-19 38.28440218895072 40.0 38.0 41.0 33.5 41.0 20-21 38.18989454914379 40.0 38.0 41.0 33.0 41.0 22-23 38.048175362006944 40.0 37.0 41.0 33.0 41.0 24-25 37.881455406277865 40.0 37.0 41.0 33.0 41.0 26-27 37.612185261475084 39.0 37.0 41.0 32.0 41.0 28-29 37.57733882620592 39.0 37.0 41.0 32.0 41.0 30-31 37.67384634303809 39.0 37.0 41.0 33.0 41.0 32-33 37.58692624588803 40.0 36.5 41.0 33.0 41.0 34-35 37.44178067451655 39.5 36.0 41.0 32.5 41.0 36-37 37.21722415224275 39.0 36.0 41.0 31.5 41.0 38-39 37.01262950779352 39.0 36.0 41.0 31.0 41.0 40-41 36.838120638239005 39.0 35.0 41.0 31.0 41.0 42-43 36.5939296584376 39.0 35.0 40.0 30.5 41.0 44-45 36.239256617579244 39.0 35.0 40.0 30.0 41.0 46-47 35.812807821194696 39.0 35.0 40.0 29.0 41.0 48-49 35.429149629538536 39.0 35.0 40.0 27.5 41.0 50-51 35.15566298767178 38.0 35.0 40.0 26.5 41.0 52-53 34.875434254619236 38.0 34.0 40.0 26.0 41.0 54-55 34.643300949980016 38.0 34.0 40.0 25.5 41.0 56-57 34.36808343837427 38.0 34.0 40.0 24.5 41.0 58-59 34.06839856119531 38.0 33.5 40.0 23.5 41.0 60-61 33.6856811264488 37.0 33.0 40.0 22.5 41.0 62-63 33.28178128939035 36.5 32.5 39.5 22.0 41.0 64-65 32.88224398192271 36.0 32.0 39.0 20.5 40.0 66-67 32.45632397700372 36.0 31.5 39.0 18.5 40.0 68-69 31.981732099486578 35.0 31.0 38.5 17.5 40.0 70-71 31.56862606450026 35.0 31.0 38.0 14.0 40.0 72-73 31.119107203246536 35.0 30.5 37.0 10.0 39.5 74-75 30.66423125403511 34.0 30.0 37.0 8.0 39.0 76-77 29.425835459771882 33.5 28.5 35.5 7.0 38.0 78-79 30.057117164202047 34.0 30.0 36.0 7.0 38.0 80-81 29.893093430073478 34.0 30.0 35.5 2.0 37.5 82-83 29.528422541273404 34.0 29.0 35.0 2.0 37.0 84-85 29.15179696867218 34.0 29.0 35.0 2.0 36.5 86-87 28.759541304147326 34.0 29.0 35.0 2.0 36.0 88-89 28.321907953392568 33.5 28.5 35.0 2.0 36.0 90-91 27.80485596581302 33.0 27.0 35.0 2.0 35.0 92-93 27.30097457496849 33.0 26.0 35.0 2.0 35.0 94-95 26.786694131029606 32.5 25.0 34.0 2.0 35.0 96-97 26.246212377409535 32.0 25.0 34.0 2.0 35.0 98-99 25.423893995757368 32.0 22.0 34.0 2.0 35.0 100-101 23.733636671073263 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.007309579903004959 1101 2 0.10629062134850642 1101 3 0.2351753537293888 1101 4 0.28007901698722293 1101 5 -0.04789708345543886 1101 6 0.044049501881396225 1101 7 0.012699472293917324 1101 8 0.046417928511203854 1101 9 0.19871554627120247 1101 10-11 0.2213764743176725 1101 12-13 0.3139806394415956 1101 14-15 0.24084356438687138 1101 16-17 0.16633865604386244 1101 18-19 0.184144283543489 1101 20-21 -0.054942124132168146 1101 22-23 -0.04638127468604836 1101 24-25 0.03172321281405743 1101 26-27 0.008096596117134425 1101 28-29 0.13298722677538422 1101 30-31 0.21262377157263046 1101 32-33 0.009322651419815031 1101 34-35 0.02754580636637627 1101 36-37 0.21753424382500697 1101 38-39 0.28190449087787783 1101 40-41 0.19945177269260483 1101 42-43 0.16389041356774925 1101 44-45 0.26480476425195576 1101 46-47 0.3952325033074473 1101 48-49 0.3933738419476427 1101 50-51 0.6281372631950504 1101 52-53 0.5880214522573795 1101 54-55 0.6966490871416298 1101 56-57 0.7672830356427696 1101 58-59 0.8734122084011275 1101 60-61 0.9238213671649831 1101 62-63 0.9225282531174557 1101 64-65 0.9029223507973683 1101 66-67 0.824603125939845 1101 68-69 0.7993938301385519 1101 70-71 0.7076486436552578 1101 72-73 0.39263987928960375 1101 74-75 0.7868983956084072 1101 76-77 0.5919516035187513 1101 78-79 0.7970033097630029 1101 80-81 0.3177240444927598 1101 82-83 0.30194929301519835 1101 84-85 0.4734745937471203 1101 86-87 0.5052094808391416 1101 88-89 0.7460489490299302 1101 90-91 0.8784551797933702 1101 92-93 0.80373763253424 1101 94-95 0.3384174458504283 1101 96-97 0.293212473404008 1101 98-99 0.1901166292126284 1101 100-101 0.3607621034584092 1102 1 0.1366094544416292 1102 2 0.13987566649821304 1102 3 0.01930895097469687 1102 4 -0.07057187080224026 1102 5 -0.1290318078501187 1102 6 -0.05924217487619643 1102 7 0.02618884631630891 1102 8 0.026415124294679515 1102 9 0.1826186150673763 1102 10-11 0.15851650123086358 1102 12-13 0.029608501699932788 1102 14-15 0.17455115744042615 1102 16-17 -0.2034592036469789 1102 18-19 -0.1353142477390037 1102 20-21 -0.15538132999261478 1102 22-23 -0.09394461082966643 1102 24-25 -0.1822048207928404 1102 26-27 -0.012665531664090679 1102 28-29 0.0020922264749287933 1102 30-31 0.06125525928233344 1102 32-33 0.0877387425240812 1102 34-35 0.13841615141247132 1102 36-37 0.07645392851453181 1102 38-39 -0.03310917512536804 1102 40-41 -0.18352408373150553 1102 42-43 -0.31381242324324177 1102 44-45 -0.15788171159445596 1102 46-47 0.06756708639874631 1102 48-49 0.22662401994254822 1102 50-51 0.08867337840829492 1102 52-53 0.21241850018216724 1102 54-55 0.4928298899123362 1102 56-57 0.3519849419637282 1102 58-59 0.26043414866403936 1102 60-61 0.4685067395339786 1102 62-63 0.3693237073732192 1102 64-65 0.05872753422772803 1102 66-67 -0.28401838312924355 1102 68-69 -0.11399320818999215 1102 70-71 -0.34181664323976335 1102 72-73 0.14637595152913718 1102 74-75 0.08184552520144805 1102 76-77 0.02010539112302112 1102 78-79 0.17702290286025857 1102 80-81 0.05064700319612214 1102 82-83 0.3902921285151244 1102 84-85 0.04909466246863303 1102 86-87 0.2759411082133916 1102 88-89 0.2658086604505563 1102 90-91 0.2956051489287823 1102 92-93 0.09085298598388292 1102 94-95 -0.19350054720458232 1102 96-97 0.04244814230979799 1102 98-99 0.26817035727282246 1102 100-101 0.3875760582595369 1103 1 0.06197621210414894 1103 2 0.10060596367733865 1103 3 -0.05122670386251116 1103 4 0.21014200273572214 1103 5 -0.1432804262667915 1103 6 -0.04491032854821242 1103 7 0.178110301362139 1103 8 -0.13452823633740252 1103 9 -0.16177015915194914 1103 10-11 0.0612841067083707 1103 12-13 0.14129758441738005 1103 14-15 0.1441882282618252 1103 16-17 0.013932100700849048 1103 18-19 -0.16772180927217306 1103 20-21 -0.10435707183073362 1103 22-23 -0.10500282597936206 1103 24-25 0.03838798387680242 1103 26-27 0.18898460039086018 1103 28-29 -0.1342239951998323 1103 30-31 -0.26560155541904606 1103 32-33 -0.04556046736561825 1103 34-35 0.15183387784787783 1103 36-37 -0.01902096994741953 1103 38-39 -0.11554225988466982 1103 40-41 -0.049006972072938026 1103 42-43 -0.15029732015160135 1103 44-45 -0.25152004748011336 1103 46-47 0.17977773069375758 1103 48-49 0.17467177056953886 1103 50-51 0.1118232219398223 1103 52-53 0.21429667671412034 1103 54-55 0.16398115386689227 1103 56-57 -0.007860642518153327 1103 58-59 -0.20148353338314706 1103 60-61 -0.27798936539976893 1103 62-63 -0.24267470574793748 1103 64-65 -0.2204868497747583 1103 66-67 -0.09777512459005777 1103 68-69 0.11235480654689667 1103 70-71 -0.015080509009788301 1103 72-73 0.3326386299142925 1103 74-75 0.05625294247290569 1103 76-77 0.25202135312670393 1103 78-79 0.39463337404120935 1103 80-81 0.16531425800669552 1103 82-83 0.03848081173859441 1103 84-85 0.033009481475669844 1103 86-87 0.44750018893337185 1103 88-89 0.6345346630694628 1103 90-91 0.25383860876853603 1103 92-93 0.6417690478610609 1103 94-95 0.4801364570344937 1103 96-97 0.26179391544161135 1103 98-99 0.3985565902665833 1103 100-101 0.5648820262556278 1104 1 -0.014691616306297561 1104 2 0.12667622013227486 1104 3 0.05216486187767089 1104 4 0.23576208347692074 1104 5 0.0023151126371061537 1104 6 0.14636352350371595 1104 7 0.014453277168826162 1104 8 0.1778174695671595 1104 9 0.2500643794905528 1104 10-11 0.24714858929674222 1104 12-13 0.17505713249904886 1104 14-15 0.12554042540974564 1104 16-17 0.021991698156632822 1104 18-19 0.011486244516568433 1104 20-21 -0.12872464756189572 1104 22-23 -0.04641463510485977 1104 24-25 -0.07613816987998945 1104 26-27 -0.07196483471123116 1104 28-29 -0.18831768983548614 1104 30-31 -0.017981314938083415 1104 32-33 0.18651055874457256 1104 34-35 0.2141342031361262 1104 36-37 0.33835138345504134 1104 38-39 0.4621293877584378 1104 40-41 0.5869878436454528 1104 42-43 0.5598476073846328 1104 44-45 0.9255424107697223 1104 46-47 0.5924545335748803 1104 48-49 0.5737071601893504 1104 50-51 1.1161525327353203 1104 52-53 1.0030226794442143 1104 54-55 0.9717724611190732 1104 56-57 0.9731430314965408 1104 58-59 1.1735237275154589 1104 60-61 0.8215466631791273 1104 62-63 0.7432445966249261 1104 64-65 0.778901419517446 1104 66-67 0.8554519817966266 1104 68-69 0.6044712858823793 1104 70-71 0.6942294151864274 1104 72-73 1.0414893022735043 1104 74-75 1.2755284500097162 1104 76-77 1.096331349931912 1104 78-79 0.9247189074268185 1104 80-81 1.2435304730454675 1104 82-83 1.1144279959621173 1104 84-85 0.954105296293708 1104 86-87 1.1654705499452511 1104 88-89 1.448196406846872 1104 90-91 1.6507810443643685 1104 92-93 1.3768660534664754 1104 94-95 1.1159606416355778 1104 96-97 1.5355461300156854 1104 98-99 1.8199446562536323 1104 100-101 2.333763993087846 1105 1 -0.09674281547883723 1105 2 0.019434855682952445 1105 3 -0.035623618308513016 1105 4 0.028573512787147592 1105 5 -0.0897507394972692 1105 6 0.020073308665438105 1105 7 -0.04833897400018117 1105 8 -0.26782650655713525 1105 9 -0.2685373400776143 1105 10-11 -0.19415067590031754 1105 12-13 -0.29330970871025386 1105 14-15 -0.44238844307992053 1105 16-17 -0.3069538273028911 1105 18-19 -0.5120123656069069 1105 20-21 -0.2789713775810796 1105 22-23 -0.2288924940485586 1105 24-25 -0.38739509232703995 1105 26-27 -0.4609686487910025 1105 28-29 -0.4445909891222328 1105 30-31 -0.7326714478921659 1105 32-33 -0.6693624776507932 1105 34-35 -0.5366011385149108 1105 36-37 -0.674671975090007 1105 38-39 -0.4141338914882269 1105 40-41 -0.22026106276953072 1105 42-43 -1.2076255108949354 1105 44-45 -0.7441275743618263 1105 46-47 -0.7441353127845005 1105 48-49 -0.750497701146223 1105 50-51 -0.8577010884856122 1105 52-53 -0.567435441097949 1105 54-55 -0.6709592388399912 1105 56-57 -0.521973545743954 1105 58-59 -0.612715417441116 1105 60-61 -0.4036321891491852 1105 62-63 -0.5169559119190481 1105 64-65 -0.45894524154661553 1105 66-67 -0.2595037360907604 1105 68-69 0.07141862141365962 1105 70-71 -0.7498280545918377 1105 72-73 -0.8745551522268968 1105 74-75 -0.6446586984808107 1105 76-77 -0.561179909145384 1105 78-79 -0.5534147793014768 1105 80-81 -0.6356149145038223 1105 82-83 -0.5555759905054458 1105 84-85 -0.5845688261447144 1105 86-87 -0.5357571743204872 1105 88-89 -0.5731850657757924 1105 90-91 -0.3894526488284704 1105 92-93 -0.7611061274545072 1105 94-95 -0.34773287442881085 1105 96-97 -0.3444180995186521 1105 98-99 -0.5664679539129374 1105 100-101 -0.5445733267130493 1106 1 -0.322851686446576 1106 2 -0.12851541313425585 1106 3 -0.18763974734076783 1106 4 0.09510577085166716 1106 5 -0.25251955670157145 1106 6 -0.05768206767864825 1106 7 -0.20875564066684404 1106 8 -0.04480903343885245 1106 9 -0.23486798523889973 1106 10-11 -0.2530048425669946 1106 12-13 -0.4527015097855198 1106 14-15 0.02745362681255159 1106 16-17 0.06841579635301542 1106 18-19 -0.06695188173593891 1106 20-21 0.0638041600533299 1106 22-23 0.14409137691918517 1106 24-25 0.22285356358694486 1106 26-27 0.4506240931444907 1106 28-29 0.3490414033508884 1106 30-31 0.1515859445809511 1106 32-33 -0.03050159055402446 1106 34-35 0.01279402277540953 1106 36-37 -0.010189448208286933 1106 38-39 -1.8899901510849304E-4 1106 40-41 -0.2443874068555445 1106 42-43 -0.1589023926153672 1106 44-45 -0.23329088081344196 1106 46-47 -0.07955197945117476 1106 48-49 -0.37650576566235117 1106 50-51 -0.06487514762539348 1106 52-53 0.045501386859044146 1106 54-55 -0.06412120120557319 1106 56-57 0.022347690815180954 1106 58-59 -0.3809445841077874 1106 60-61 -0.026834474095430494 1106 62-63 -0.0029102129943154864 1106 64-65 -0.2747214512240319 1106 66-67 0.1661515058447236 1106 68-69 0.3985187558222627 1106 70-71 0.15496025185977302 1106 72-73 -0.04367477588280977 1106 74-75 -0.05352966622274735 1106 76-77 0.11284562848902269 1106 78-79 0.4938634465049745 1106 80-81 0.3460887145284346 1106 82-83 -0.08432263029038722 1106 84-85 0.31437270611334966 1106 86-87 0.9024820729913436 1106 88-89 0.5129540471274332 1106 90-91 0.4709808189134641 1106 92-93 0.6504194101799001 1106 94-95 0.43099696428086887 1106 96-97 0.5845973574705958 1106 98-99 0.8476281482376002 1106 100-101 1.1772008668353386 1107 1 0.3059089067737588 1107 2 0.3209019597471041 1107 3 0.08969076113380936 1107 4 0.07963966915675513 1107 5 0.11125162973910818 1107 6 0.0045001357111829066 1107 7 -0.08449716609057134 1107 8 0.11901511910351559 1107 9 0.05549218425262126 1107 10-11 0.3031657082804742 1107 12-13 0.11829954953651622 1107 14-15 0.40258604206679394 1107 16-17 0.2897611353360716 1107 18-19 0.10619430470473645 1107 20-21 0.2333486515787513 1107 22-23 0.42608290234291246 1107 24-25 0.390279411044574 1107 26-27 0.37440587280550375 1107 28-29 -0.14002109664911444 1107 30-31 0.315118783564003 1107 32-33 0.4004570958866509 1107 34-35 0.31311181938558263 1107 36-37 0.7516219924696799 1107 38-39 0.41882583149337194 1107 40-41 -0.003603508550462209 1107 42-43 0.19205099721514074 1107 44-45 0.2166296700340169 1107 46-47 0.11165564766746883 1107 48-49 0.008345929252904227 1107 50-51 0.07784201339155317 1107 52-53 0.4044526580454786 1107 54-55 0.3133946886249319 1107 56-57 0.06932862301857057 1107 58-59 0.31553317012949833 1107 60-61 0.4588381953961047 1107 62-63 0.7357550412429745 1107 64-65 0.5379109640302033 1107 66-67 0.8851928194040397 1107 68-69 1.082549476161244 1107 70-71 0.35975368406316477 1107 72-73 1.0058485292019377 1107 74-75 1.1295211812348782 1107 76-77 1.0363096115398704 1107 78-79 1.0569460736236174 1107 80-81 1.413646977240301 1107 82-83 1.3210004629299519 1107 84-85 1.0696957993336902 1107 86-87 1.4309778357032066 1107 88-89 1.2019106149240422 1107 90-91 1.1422996748456704 1107 92-93 1.7829935627222753 1107 94-95 1.5550965405520536 1107 96-97 1.8538505354366954 1107 98-99 2.0162457329833607 1107 100-101 1.914912731242115 1108 1 -0.2962829113461751 1108 2 -0.3529255536965046 1108 3 -0.4937140445295256 1108 4 -0.07934202031299264 1108 5 -0.35176654465337975 1108 6 -0.22532765000796928 1108 7 -0.12866025914074442 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12 962.0 13 1308.0 14 1712.0 15 2289.0 16 3071.0 17 4142.0 18 3672.0 19 2632.0 20 2162.0 21 2169.0 22 2194.0 23 2393.0 24 2804.0 25 3182.0 26 3904.0 27 4858.0 28 5839.0 29 7134.0 30 8830.0 31 11070.0 32 13998.0 33 17796.0 34 24326.0 35 33723.0 36 48630.0 37 64210.0 38 41814.0 39 3003.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.482709514303906 21.300992553541604 16.2373393290498 20.97895860310469 2 16.224060011682603 21.775448089279674 39.4069542226458 22.59353767639192 3 19.516709195437638 25.601500292065055 31.101853844498418 23.779936667998893 4 13.123866326436499 18.46681218679866 37.500845451471086 30.90847603529376 5 12.890371577474749 38.53490552984765 34.43171860347133 14.143004289206266 6 30.82485319888093 38.12463491868294 16.834014818458513 14.216497063977618 7 26.982199403572416 34.4673655732161 20.692655332493008 17.85777969071848 8 26.153964398807144 35.821625111445876 19.323638823131553 18.700771666615427 9 26.07894979555446 16.980354782180957 20.48882466873674 36.451870753527835 10-11 24.56220985642697 27.872229224951578 27.23506625265164 20.33049466596981 12-13 25.790543303479467 25.24957648093319 27.75379629885842 21.206083916728925 14-15 22.378178128939037 26.6478617763704 26.73025486518892 24.243705229501643 16-17 20.83545977188182 29.972484397577396 27.60506656008854 21.58698927045224 18-19 20.73892458572878 27.64288129861346 31.243274817843634 20.374919297814124 20-21 21.372552033695083 26.6649245242414 31.78482491468626 20.177698527377256 22-23 21.25730553668217 26.613213144893272 31.592045919198693 20.537435399225867 24-25 21.250257479194143 26.924945199204352 31.453350918777996 20.37144640282351 26-27 20.735029176120467 27.42001930727466 31.339119366918155 20.505832149686718 28-29 20.539654772207765 27.707535582689115 31.418526825174055 20.334282819929072 30-31 20.843485171526996 27.34132772977485 31.186600649615166 20.62858644908299 32-33 20.56045746610508 27.3537368955022 30.98625757063363 21.09954806775909 34-35 21.191779137332063 27.377102099794016 30.934147016324896 20.49697174654902 36-37 21.61118950776127 27.041156704143336 30.725338104768667 20.622315683326722 38-39 20.991516265742717 27.391440021274665 31.002445664287613 20.614598048695004 40-41 21.286623420542934 27.30823623451287 30.933070987179885 20.47206935776432 42-43 21.070376024462576 27.49656782937692 30.589134201330438 20.84392194483007 44-45 21.137209087834723 27.809973252989824 30.499738678636213 20.553078980539244 46-47 21.66584683493713 28.060380606880436 29.58326928397946 20.69050327420297 48-49 21.38623297568174 28.421157807360036 29.340394134103974 20.852215082854244 50-51 21.471700433486028 28.566882897285335 29.231561472007872 20.729855197220772 52-53 21.7499642604056 28.364325582324813 29.22222956655942 20.663480590710165 54-55 21.427890675438867 28.623758723522 29.30150336643404 20.646847234605097 56-57 21.576843852799215 28.676637870077165 28.93872782611369 20.80779045100993 58-59 21.247578934423707 28.306176407292405 29.17699142251053 21.26925323577336 60-61 21.018384726534876 28.52876072186184 29.39142865926769 21.0614258923356 62-63 21.205152642420146 28.412549574199897 29.612629507793525 20.769668275586437 64-65 20.84652750023058 28.679558520613647 29.277984443692933 21.195929535462845 66-67 20.88464967565407 28.49694100285916 29.321179327942943 21.297229993543827 68-69 21.29246472161589 28.268822824115354 29.17791373320626 21.2607987210625 70-71 21.38269745134811 28.275432717434747 28.797768008116336 21.54410182310081 72-73 21.08848033941034 27.98705690656993 29.094905770590586 21.829556983429153 74-75 21.131982660558922 28.25329726073723 28.743044240169706 21.87167583853414 76-77 21.098472038614073 28.078826820795033 29.00759369139484 21.815107449196052 78-79 21.078027484858733 28.23946260030129 28.882928029022043 21.799581885817936 80-81 20.931994958034863 28.39348848648815 28.77578626986811 21.898730285608877 82-83 20.800232423724434 28.298818205295323 28.823616218010994 22.077333152969246 84-85 20.66655496442181 28.391841227044033 28.732481834294333 22.209121974239824 86-87 20.897562025394286 28.236080794416946 28.60193070372306 22.264426476465704 88-89 20.55615334952501 27.87438128324162 28.76318135702647 22.806284010206905 90-91 20.419351218559804 28.123803769825713 28.667247775107956 22.789597236506523 92-93 20.44139868997714 28.060191568937608 28.794062623458355 22.704347117626895 94-95 20.057951855381685 28.11741015156639 28.500630245642082 23.324007747409844 96-97 20.28376425738617 28.222707289328863 28.136471239278137 23.357057214006822 98-99 20.06689940033419 28.69280231934318 27.679319446173807 23.560978834148823 100-101 20.26445940482029 28.82564466015537 27.62587649644328 23.284019438581062 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 144.0 1 97.5 2 68.5 3 94.5 4 99.0 5 103.0 6 155.5 7 315.0 8 497.0 9 596.5 10 592.5 11 549.0 12 557.0 13 598.5 14 621.5 15 636.5 16 677.0 17 709.5 18 757.0 19 818.0 20 900.0 21 1059.0 22 1239.5 23 1419.5 24 1701.0 25 2119.0 26 2681.5 27 3398.5 28 4037.0 29 4645.0 30 5288.0 31 6001.5 32 6939.5 33 7514.0 34 7779.0 35 8309.0 36 9093.0 37 9676.0 38 10125.5 39 10311.5 40 10358.5 41 10883.5 42 10956.0 43 10888.0 44 11242.0 45 11382.0 46 11272.0 47 11380.0 48 11816.5 49 11464.0 50 10679.0 51 10281.0 52 9893.0 53 9562.0 54 9437.0 55 8656.0 56 7409.5 57 6579.0 58 6182.0 59 5358.0 60 3979.0 61 3268.5 62 2726.0 63 1878.5 64 1226.5 65 861.5 66 648.0 67 532.5 68 442.0 69 354.0 70 265.0 71 173.5 72 116.0 73 80.0 74 50.0 75 48.0 76 33.5 77 20.5 78 15.5 79 9.0 80 5.0 81 2.5 82 1.0 83 1.5 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04580809788790851 2 0.0 3 0.0 4 0.0 5 0.010760291450179852 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005841301072954776 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.223106957297015E-4 24-25 3.074368985765671E-4 26-27 0.0012297475943062685 28-29 0.0019983398407476863 30-31 1.5371844928828356E-4 32-33 0.0 34-35 0.0 36-37 3.074368985765671E-4 38-39 1.5371844928828356E-4 40-41 0.0 42-43 0.011836320595197835 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.5371844928828356E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.148737971531342E-4 84-85 1.5371844928828356E-4 86-87 0.0 88-89 0.0 90-91 0.009069388508008732 92-93 0.002613213637900821 94-95 0.0 96-97 0.0 98-99 0.0016909029421711195 100-101 0.01245119439235097 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 325270.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.83750867228305 #Duplication Level Percentage of deduplicated Percentage of total 1 88.03095060308158 64.11955127981275 2 7.50720891617473 10.93612789073035 3 1.9233549691411826 4.202771527340987 4 0.8129775872922259 2.3686104825907703 5 0.4312956772785114 1.5707251317045885 6 0.27211335213832794 1.1892035187731684 7 0.1817096191908549 0.9264693174555799 8 0.1391322562776842 0.8107237538576104 9 0.11272488462076279 0.7389539785032224 >10 0.536483911508755 6.996574061861785 >50 0.030467252660743356 1.5182744652459712 >100 0.019465189199886504 2.7021340310178927 >500 8.463125739081089E-4 0.39790749789484425 >1k 0.0012694688608621634 1.5219730632104886 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1980 0.608725059181603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1792 0.5509269222492084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1166 0.3584714237402773 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 672 0.20659759584345314 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 619 0.1903034402188951 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 477 0.14664740062102255 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 451 0.1386540412580318 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 370 0.11375165247332986 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 345 0.10606573000891567 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.537184492882836E-4 3.074368985765672E-4 18-19 0.0 0.0 0.0 3.074368985765672E-4 3.074368985765672E-4 20-21 0.0 0.0 0.0 3.074368985765672E-4 3.074368985765672E-4 22-23 0.0 0.0 0.0 6.148737971531343E-4 3.074368985765672E-4 24-25 0.0 0.0 0.0 6.148737971531343E-4 3.074368985765672E-4 26-27 0.0 0.0 0.0 9.223106957297015E-4 3.074368985765672E-4 28-29 0.0 0.0 0.0 0.0030743689857656714 3.074368985765672E-4 30-31 0.0 0.0 0.0 0.007839640913702463 3.074368985765672E-4 32-33 0.0 0.0 0.0 0.01752390321886433 3.074368985765672E-4 34-35 0.0 0.0 0.0 0.031051126756233283 3.074368985765672E-4 36-37 0.0 0.0 0.0 0.04949734067082731 3.074368985765672E-4 38-39 0.0 0.0 0.0 0.09007901128293419 3.074368985765672E-4 40-41 0.0 0.0 0.0 0.1329664586343653 3.074368985765672E-4 42-43 0.0 0.0 0.0 0.19307037230608418 3.074368985765672E-4 44-45 0.0 0.0 0.0 0.2714667814431088 3.074368985765672E-4 46-47 0.0 0.0 0.0 0.3666185015525564 3.074368985765672E-4 48-49 0.0 0.0 0.0 0.4683801149814001 3.074368985765672E-4 50-51 0.0 0.0 0.0 0.6007316998186122 3.074368985765672E-4 52-53 0.0 0.0 0.0 0.7463030712946168 3.074368985765672E-4 54-55 0.0 0.0 0.0 0.9007901128293418 3.074368985765672E-4 56-57 0.0 0.0 0.0 1.1336735635010915 3.074368985765672E-4 58-59 0.0 0.0 0.0 1.3780858978694623 3.074368985765672E-4 60-61 0.0 0.0 0.0 1.6427890675438865 3.074368985765672E-4 62-63 0.0 0.0 0.0 1.9526854613090663 3.074368985765672E-4 64-65 0.0 0.0 0.0 2.264580194914994 3.074368985765672E-4 66-67 0.0 0.0 0.0 2.6491837550342794 3.074368985765672E-4 68-69 0.0 0.0 0.0 3.124481200233652 3.074368985765672E-4 70-71 0.0 0.0 0.0 3.692624588803148 3.074368985765672E-4 72-73 0.0 0.0 0.0 4.335167706828173 3.074368985765672E-4 74-75 0.0 0.0 0.0 4.967411688750884 3.074368985765672E-4 76-77 0.0 0.0 0.0 5.672671934085529 3.074368985765672E-4 78-79 0.0 0.0 0.0 6.541796046361484 3.074368985765672E-4 80-81 0.0 0.0 0.0 7.527131306299382 4.6115534786485073E-4 82-83 0.0 0.0 0.0 8.467734497494389 6.148737971531343E-4 84-85 0.0 0.0 0.0 9.533618224859348 6.148737971531343E-4 86-87 0.0 0.0 0.0 10.711562701755465 6.148737971531343E-4 88-89 0.0 0.0 0.0 12.03292649183755 6.148737971531343E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACCG 35 2.7832057E-4 54.292656 5 GGTATCA 1140 0.0 47.118336 1 GTATCAA 1905 0.0 37.429375 1 GTAAGGT 180 0.0 36.94917 4 GGTAAGG 200 0.0 35.62956 3 AGGTAAG 205 0.0 32.443172 2 ACCGAGT 75 3.074099E-4 31.665846 8 GTATAGG 95 3.465604E-5 30.022299 1 ATCAACG 2405 0.0 29.629568 3 TATCAAC 2410 0.0 29.568094 2 TAAGGTG 275 0.0 29.367388 5 TCAACGC 2435 0.0 29.26452 4 GTACCTA 65 0.0058343466 29.234507 5 CAACGCA 2460 0.0 28.96712 5 AACGCAG 2510 0.0 28.574957 6 GTAGATC 150 3.317109E-8 28.503643 3 CTTATAG 85 6.392446E-4 27.94475 3 AAAGGCG 85 6.392446E-4 27.94475 5 GGTGTGC 275 0.0 27.635647 8 ACGCAGA 2765 0.0 25.596083 7 >>END_MODULE