FastQCFastQC Report
Thu 26 May 2016
SRR937165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418066
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28010.6699899059000254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.5044658020503939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.3547286792037621No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5630.13466773188922324No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.13323255179804144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.046.051791
GTATCAA22250.039.535461
ATCAACG28100.030.9626223
TCAACGC28100.030.9626224
TATCAAC28150.030.9076252
CAACGCA28650.030.2022785
AACGCAG29500.029.3074156
ATGGGAG4600.027.9060845
ACGCAGA31400.027.6853227
CGCAGAG31850.027.1450168
GTACATG17400.026.2341541
GCAGAGT33600.025.44549
TACATGG17250.025.356642
GAGTACT20050.025.34810312-13
AGTACTT20900.024.2035712-13
ACATGGG17550.024.1104773
GTACTTT21550.023.79843514-15
TAAGGGT1000.001643064223.771854
ATACTGC1252.251342E-422.8018156
ATTACAC2103.3995093E-822.6398563
Produced by FastQC (version 0.11.5)