##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937165_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 418066 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.904658594575977 33.0 31.0 34.0 31.0 34.0 2 32.018671692986274 33.0 31.0 34.0 30.0 34.0 3 32.06265278688054 34.0 31.0 34.0 30.0 34.0 4 35.67896695737037 37.0 35.0 37.0 35.0 37.0 5 35.67049221893194 37.0 35.0 37.0 35.0 37.0 6 35.73567331473978 37.0 35.0 37.0 35.0 37.0 7 35.69920060468921 37.0 35.0 37.0 35.0 37.0 8 35.69340486908766 37.0 35.0 37.0 35.0 37.0 9 37.36101955193678 39.0 37.0 39.0 35.0 39.0 10-11 37.279326709179884 39.0 37.0 39.0 35.0 39.0 12-13 37.17417943578286 39.0 37.0 39.0 34.0 39.0 14-15 38.38522147220774 40.0 38.0 41.0 34.0 41.0 16-17 38.33922036233513 40.0 38.0 41.0 34.0 41.0 18-19 38.320125291221956 40.0 38.0 41.0 34.0 41.0 20-21 38.26485770189396 40.0 38.0 41.0 34.0 41.0 22-23 38.141697722369194 40.0 38.0 41.0 34.0 41.0 24-25 38.009371725995415 40.0 38.0 41.0 33.5 41.0 26-27 37.83363511981362 40.0 38.0 41.0 33.0 41.0 28-29 37.642664076963925 40.0 37.5 41.0 33.0 41.0 30-31 37.59099400573115 40.0 37.0 41.0 33.0 41.0 32-33 37.39025058244392 40.0 37.0 41.0 33.0 41.0 34-35 37.1921646342922 40.0 36.5 41.0 32.0 41.0 36-37 37.181814593867955 40.0 37.0 41.0 32.0 41.0 38-39 37.151014433127784 40.0 37.0 41.0 32.0 41.0 40-41 36.97215750623107 40.0 36.5 41.0 31.5 41.0 42-43 36.72983452373549 40.0 36.0 41.0 31.0 41.0 44-45 36.470772557443084 40.0 36.0 41.0 30.5 41.0 46-47 36.09903340620859 39.0 36.0 41.0 29.5 41.0 48-49 35.72091129151856 39.0 35.0 41.0 27.5 41.0 50-51 34.775524677921666 38.0 34.0 40.0 25.5 40.5 52-53 34.887141025579695 38.5 35.0 40.0 26.5 40.5 54-55 35.22840891151158 39.0 35.0 40.5 26.0 41.0 56-57 35.010975539747314 39.0 35.0 40.5 25.5 41.0 58-59 34.78520257566987 38.5 35.0 40.0 25.0 41.0 60-61 34.52660225897346 38.0 34.5 40.0 24.5 41.0 62-63 34.205796453191596 38.0 34.0 40.0 23.0 41.0 64-65 33.857557179966804 37.5 34.0 40.0 22.0 41.0 66-67 33.4924784603388 37.0 34.0 40.0 21.5 41.0 68-69 33.00652887343146 36.0 33.0 39.0 19.0 41.0 70-71 32.583571732692924 36.0 33.0 39.0 17.5 40.5 72-73 32.18384776566379 35.0 32.0 38.5 13.5 40.0 74-75 31.74089856625509 35.0 32.0 37.5 9.0 40.0 76-77 31.31673707022336 35.0 31.5 37.0 7.0 39.0 78-79 30.863708122640922 35.0 31.0 36.5 2.0 39.0 80-81 30.431514402032214 35.0 31.0 36.0 2.0 38.5 82-83 29.98526189644697 34.0 30.5 36.0 2.0 37.0 84-85 29.53946864849091 34.0 30.0 35.0 2.0 37.0 86-87 29.130149306568818 34.0 29.5 35.0 2.0 36.5 88-89 28.740826807250528 34.0 29.0 35.0 2.0 36.0 90-91 28.3331064951467 34.0 29.0 35.0 2.0 36.0 92-93 27.914078159907767 34.0 28.5 35.0 2.0 35.0 94-95 27.411042754014915 34.0 27.0 35.0 2.0 35.0 96-97 26.8391904627499 33.5 25.5 35.0 2.0 35.0 98-99 26.197705625427563 33.0 25.0 35.0 2.0 35.0 100-101 24.411513971478186 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13153683316696885 1101 2 0.16768996583004636 1101 3 0.13627777850401301 1101 4 0.09056786079162293 1101 5 0.14971213366646907 1101 6 -0.0049109128290680815 1101 7 0.08758582096749734 1101 8 0.24448296892292376 1101 9 0.1109327651394807 1101 10-11 0.03698805240395586 1101 12-13 0.19096107006595275 1101 14-15 0.22757173994825308 1101 16-17 0.145007777515211 1101 18-19 0.1660807259359771 1101 20-21 0.2982708676593333 1101 22-23 0.2988575483927107 1101 24-25 0.2684892898060198 1101 26-27 0.27910789215641074 1101 28-29 0.20890767077945327 1101 30-31 0.2720319312138031 1101 32-33 0.31778740117690774 1101 34-35 0.2795242253082435 1101 36-37 0.2572119696713031 1101 38-39 0.3739309059537632 1101 40-41 0.4020783233346066 1101 42-43 0.42574352733034004 1101 44-45 0.6660696683262657 1101 46-47 0.813427240457159 1101 48-49 1.1239560182300536 1101 50-51 0.8723286957658871 1101 52-53 0.7096367270758535 1101 54-55 0.8325051700152812 1101 56-57 0.9211741572665915 1101 58-59 0.9805135453560325 1101 60-61 0.9842633754750381 1101 62-63 1.2082947149823653 1101 64-65 1.2604619593402973 1101 66-67 1.0258136275904164 1101 68-69 1.1096747139359806 1101 70-71 0.9412936468542625 1101 72-73 0.9609582358546902 1101 74-75 1.0617257534054865 1101 76-77 1.0353525826047694 1101 78-79 1.3173372634924227 1101 80-81 1.6379383255543019 1101 82-83 1.5734232184213255 1101 84-85 1.371118830776556 1101 86-87 1.5581083645062073 1101 88-89 1.6252487882387427 1101 90-91 1.5391808881753661 1101 92-93 1.389414924657565 1101 94-95 1.280364423144352 1101 96-97 1.4265229906847452 1101 98-99 1.650520979529606 1101 100-101 1.6845084181886953 1102 1 0.10946201768769015 1102 2 0.01627596271404741 1102 3 0.14942006010859998 1102 4 -0.07828000600723328 1102 5 -0.01403524999498984 1102 6 0.1147529368475304 1102 7 0.2181037379685833 1102 8 0.08102830043071663 1102 9 0.15543333955366023 1102 10-11 0.10907624465173171 1102 12-13 0.20124308382041534 1102 14-15 0.32112335066855735 1102 16-17 0.2766863258997674 1102 18-19 0.408075910299317 1102 20-21 0.3343897163130052 1102 22-23 0.30915529952186205 1102 24-25 0.39444730249034166 1102 26-27 0.34755130842570736 1102 28-29 0.4034538835802337 1102 30-31 0.3187750815214656 1102 32-33 0.2562062671416854 1102 34-35 0.18112156883939434 1102 36-37 0.17625812739127156 1102 38-39 0.47012246127849977 1102 40-41 0.5417420156374533 1102 42-43 0.292842184932276 1102 44-45 0.4592726961971536 1102 46-47 0.5579286017400662 1102 48-49 0.7881078524210778 1102 50-51 0.8628799760013237 1102 52-53 0.6658144335108673 1102 54-55 0.8644425982135147 1102 56-57 0.9665497297135914 1102 58-59 0.9003402768606819 1102 60-61 0.8573194162663142 1102 62-63 1.0139043021910226 1102 64-65 0.7262575623178051 1102 66-67 0.8079729527717987 1102 68-69 1.04179390881189 1102 70-71 0.802536427333628 1102 72-73 1.0118678561118344 1102 74-75 0.8406840965746802 1102 76-77 0.8097014487269227 1102 78-79 1.0551541748752662 1102 80-81 0.9316992433579934 1102 82-83 1.1222279648135292 1102 84-85 1.1373479984268116 1102 86-87 0.8692032136634715 1102 88-89 1.2513744039635277 1102 90-91 1.1134203795434168 1102 92-93 0.9978273382987197 1102 94-95 1.0353035981911702 1102 96-97 1.050478422439621 1102 98-99 1.2234582031419663 1102 100-101 1.7288468976435531 1103 1 0.001210751045878311 1103 2 0.0509676917075339 1103 3 -0.030500103074349028 1103 4 0.07296031597201846 1103 5 -0.13963347779311874 1103 6 0.04396136796633954 1103 7 0.2753076947163322 1103 8 0.013004700316663786 1103 9 0.21678266949465552 1103 10-11 0.1685108069748935 1103 12-13 0.13495768913263362 1103 14-15 0.12481361013821157 1103 16-17 0.10385826018792699 1103 18-19 0.1490514986571867 1103 20-21 0.27963569705566016 1103 22-23 0.17390537080473933 1103 24-25 0.15833759266646297 1103 26-27 0.09712470785105864 1103 28-29 -0.03535141547883569 1103 30-31 0.01212713117821096 1103 32-33 0.04358481209376208 1103 34-35 -0.051455800493613424 1103 36-37 -0.11582508713727435 1103 38-39 0.14615386960873167 1103 40-41 0.18087631256899783 1103 42-43 0.26208856047237816 1103 44-45 0.48891321118848197 1103 46-47 0.26728639789298114 1103 48-49 0.1483254296807459 1103 50-51 -0.0204200919915678 1103 52-53 -0.04215828159890833 1103 54-55 -0.04667094991887666 1103 56-57 0.07945521959142354 1103 58-59 0.09013585951591097 1103 60-61 0.0766162381468618 1103 62-63 0.2001181946745234 1103 64-65 0.06828704052718138 1103 66-67 0.11831774254215333 1103 68-69 0.38362818161692047 1103 70-71 0.4187615057447829 1103 72-73 0.5226469230738644 1103 74-75 0.4943206197251655 1103 76-77 0.39982164261633457 1103 78-79 0.2625127606230784 1103 80-81 0.45209820811135515 1103 82-83 0.4268953479644537 1103 84-85 0.2800738554662665 1103 86-87 0.3610177465961577 1103 88-89 0.45760343034915607 1103 90-91 0.41304093533048913 1103 92-93 0.20909551543117288 1103 94-95 0.13050900252955344 1103 96-97 0.2651056678501007 1103 98-99 0.7233978868653423 1103 100-101 1.2122632115513916 1104 1 -0.05804429324545879 1104 2 0.062492667101235355 1104 3 0.07262797271305388 1104 4 0.09135352463343338 1104 5 -0.027271888226188423 1104 6 0.08429724395059424 1104 7 0.13611601164546272 1104 8 0.03984402118280883 1104 9 0.10404275807733399 1104 10-11 0.022412443293788442 1104 12-13 0.031514345136571364 1104 14-15 0.1989523896657701 1104 16-17 0.18507442652650496 1104 18-19 0.033288088224111334 1104 20-21 0.029846388492664744 1104 22-23 0.10597045446615283 1104 24-25 -0.0637019619595165 1104 26-27 0.25385299279200524 1104 28-29 -0.21380706262450389 1104 30-31 0.023815123304196106 1104 32-33 0.09735572744809673 1104 34-35 -0.08918494665503118 1104 36-37 -0.19949265603491284 1104 38-39 -0.032132609229854836 1104 40-41 -0.10616231932075948 1104 42-43 -0.052822856850454514 1104 44-45 -0.06261699304379675 1104 46-47 0.006867168070151308 1104 48-49 -0.0011532243546881205 1104 50-51 -0.26530813333015146 1104 52-53 -0.05375707589025325 1104 54-55 -0.23704373535194634 1104 56-57 -0.15632849103849367 1104 58-59 -0.09713807011006992 1104 60-61 -0.038359770711402064 1104 62-63 0.14262557656428498 1104 64-65 -0.14982780937439344 1104 66-67 -0.06299838688698145 1104 68-69 0.0739579060263722 1104 70-71 -0.16061533972765574 1104 72-73 -0.038301342182037956 1104 74-75 0.027223855453510026 1104 76-77 -0.3030003923246092 1104 78-79 -0.12502568671944303 1104 80-81 -0.032605901140613014 1104 82-83 0.09393213438177739 1104 84-85 -0.11011130693530546 1104 86-87 -0.20953927850226606 1104 88-89 0.12895862227829014 1104 90-91 -0.02159550659864351 1104 92-93 -0.19419096340740793 1104 94-95 0.004684691505932648 1104 96-97 0.1355073656847452 1104 98-99 0.33276519739683863 1104 100-101 0.49539405554745386 1105 1 -0.1565641699461402 1105 2 -0.1993456619803986 1105 3 -0.10255538187878344 1105 4 0.015726398783094453 1105 5 0.14094930650170312 1105 6 -0.2032505876820565 1105 7 -0.1956495070620221 1105 8 0.08653300927804963 1105 9 -0.16426612797708628 1105 10-11 0.01400869116793757 1105 12-13 0.012747072602557807 1105 14-15 0.029203240005905684 1105 16-17 -0.4392479586775764 1105 18-19 -0.05710041092554974 1105 20-21 -0.15308967655835914 1105 22-23 0.09320206586070867 1105 24-25 0.2408460291119141 1105 26-27 0.39150844559813436 1105 28-29 0.08848221246052645 1105 30-31 0.08812209609330779 1105 32-33 -0.42169720622536744 1105 34-35 0.02346519152523996 1105 36-37 0.010780515733792129 1105 38-39 -0.0655018162876786 1105 40-41 -0.15088489585136955 1105 42-43 -0.05643680242869209 1105 44-45 0.07723074292558607 1105 46-47 0.19467940233885628 1105 48-49 -0.2293300419567288 1105 50-51 -0.1001029887723277 1105 52-53 -0.2023141549718801 1105 54-55 -0.11982966222269198 1105 56-57 0.006963388213208077 1105 58-59 -0.38653937750802925 1105 60-61 -0.2171836440107242 1105 62-63 -0.4521342023472812 1105 64-65 -0.7529647672825561 1105 66-67 -0.1477998856114695 1105 68-69 -0.3289970259464141 1105 70-71 -0.32718900044194044 1105 72-73 -0.4013500773010854 1105 74-75 -0.11294275806689669 1105 76-77 -0.19966413594705656 1105 78-79 -0.5163880910561751 1105 80-81 -0.731864510834491 1105 82-83 -0.46212388177468355 1105 84-85 -0.6728106054046954 1105 86-87 -0.6922202415124694 1105 88-89 -0.9266190775516421 1105 90-91 -0.8340290737755147 1105 92-93 -0.8644649323699909 1105 94-95 -0.9930300193784198 1105 96-97 -1.0496674855057293 1105 98-99 -0.8770761610215274 1105 100-101 -0.5022608657960852 1106 1 -0.5063672490725253 1106 2 -0.566692600755907 1106 3 -0.423715424843337 1106 4 -0.4900022084492761 1106 5 -0.40810189292543697 1106 6 -0.38477582935767884 1106 7 -0.2352127007605489 1106 8 -0.35456974761418536 1106 9 -0.3440011798925937 1106 10-11 -0.3348992930783936 1106 12-13 -0.44357229725064684 1106 14-15 -0.48467073063498134 1106 16-17 -0.7899461326840225 1106 18-19 -0.5645296984300217 1106 20-21 -0.5687717388569595 1106 22-23 -0.2127413641428717 1106 24-25 -0.7196802866775656 1106 26-27 -0.3418427896328069 1106 28-29 -0.2913960546357828 1106 30-31 -0.2718850646657316 1106 32-33 -0.1534372706722067 1106 34-35 -0.1828541783279647 1106 36-37 -0.3113641315988289 1106 38-39 -0.7874853465418852 1106 40-41 -0.6363843587944444 1106 42-43 -0.569505187677521 1106 44-45 -0.4327048102430453 1106 46-47 -0.18949532018172732 1106 48-49 -0.16971672294491214 1106 50-51 0.010978285842782043 1106 52-53 0.13146672580147367 1106 54-55 0.3220786800615869 1106 56-57 -0.03958154554977256 1106 58-59 0.14783226588617282 1106 60-61 0.5987848486495011 1106 62-63 0.5316645803236852 1106 64-65 0.18285692981734059 1106 66-67 0.3344772357633943 1106 68-69 0.4958329060263722 1106 70-71 0.48860047324227196 1106 72-73 1.01844942144578 1106 74-75 0.772664116261776 1106 76-77 0.2756312453633605 1106 78-79 0.5190576661940902 1106 80-81 0.4050850058142714 1106 82-83 0.05685212968252884 1106 84-85 0.1341174289669489 1106 86-87 0.5438162783586371 1106 88-89 0.59369957622566 1106 90-91 0.5348395262960963 1106 92-93 0.98560667522041 1106 94-95 0.9095836576713445 1106 96-97 0.6426884042185783 1106 98-99 0.4564361912878141 1106 100-101 0.8791390625963267 1107 1 -0.17330419929120922 1107 2 -0.2767766343693516 1107 3 -0.4801064022869461 1107 4 -0.20669590347114308 1107 5 0.02010096716596621 1107 6 -0.11134715964417552 1107 7 -0.3078944050212016 1107 8 -0.15483020267340208 1107 9 -0.16033122068083827 1107 10-11 -0.05511895989248217 1107 12-13 -0.03994066916081351 1107 14-15 0.0675185661363571 1107 16-17 -0.06909989945388162 1107 18-19 0.12937417891038194 1107 20-21 -0.02623893626223861 1107 22-23 0.014837386655692342 1107 24-25 -0.1877253994595165 1107 26-27 -0.10652410075374519 1107 28-29 -0.0559422239807148 1107 30-31 0.18956267232380952 1107 32-33 0.4403142650297909 1107 34-35 0.4203572816946419 1107 36-37 -0.11056802368197083 1107 38-39 0.4560401338257023 1107 40-41 0.5418922149929699 1107 42-43 0.5282637444567371 1107 44-45 0.63928889952156 1107 46-47 0.7465334773021794 1107 48-49 0.5276394124917161 1107 50-51 0.520511923859388 1107 52-53 0.8558757876718417 1107 54-55 0.9970504231447848 1107 56-57 0.7269180367392849 1107 58-59 1.041131690919336 1107 60-61 0.7521707644193114 1107 62-63 1.0639388935577472 1107 64-65 1.3811222008380284 1107 66-67 1.1743956592731521 1107 68-69 1.0138067622355251 1107 70-71 1.3196363963834585 1107 72-73 1.366857205612078 1107 74-75 1.2998890413195205 1107 76-77 1.3900717584106808 1107 78-79 1.5322647033949401 1107 80-81 1.624678106212329 1107 82-83 1.6582576041530181 1107 84-85 1.6948698000908387 1107 86-87 1.5468753967428306 1107 88-89 1.9871464286508385 1107 90-91 1.5227136233033178 1107 92-93 1.8863020410860578 1107 94-95 1.3446050346431875 1107 96-97 0.9281569776128507 1107 98-99 0.8784593865308281 1107 100-101 0.8691877136356858 1108 1 -0.15657902304895188 1108 2 -0.07449047973282674 1108 3 0.004609754918295295 1108 4 0.010260456947847274 1108 5 -0.19468625776767112 1108 6 -0.1471949776651229 1108 7 -0.15651158114929586 1108 8 -0.2083307877691567 1108 9 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-0.36443855412487025 2316 88-89 -0.1792385161316723 2316 90-91 0.16520855230940157 2316 92-93 0.34618484478224687 2316 94-95 0.17557190414961 2316 96-97 0.8096647531368504 2316 98-99 0.492049650222512 2316 100-101 0.3303100936221668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1960.0 3 355.0 4 303.0 5 352.0 6 327.0 7 375.0 8 466.0 9 570.0 10 721.0 11 926.0 12 1359.0 13 1691.0 14 2168.0 15 2755.0 16 3552.0 17 5056.0 18 4119.0 19 2685.0 20 2484.0 21 2407.0 22 2513.0 23 2899.0 24 3050.0 25 3314.0 26 3887.0 27 4473.0 28 5368.0 29 6521.0 30 8037.0 31 10259.0 32 13424.0 33 18053.0 34 25704.0 35 38688.0 36 58882.0 37 86172.0 38 78593.0 39 13589.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71163259429888 19.43708609271523 15.712640368557443 22.13864094442845 2 14.871013780117773 20.248737722023 41.45284897038894 23.427399527470286 3 18.761064069658662 24.9617405070882 30.74264194391806 25.534553479335077 4 12.057050610108588 16.870036196338148 38.29615752723121 32.77675566632205 5 12.720234469062843 37.603311731607754 35.1595665584182 14.516887240911206 6 30.422757329767013 37.16089416806276 17.909006152378623 14.507342349791605 7 26.90167954227859 33.63087462278013 21.386778907806146 18.08066692713514 8 25.824652569713763 34.60304981232293 20.366437683063044 19.205859934900264 9 25.767043207524104 16.759064184517 21.071907079974785 36.4019855279841 10-11 23.889568052159742 27.186945682918644 27.975454240375857 20.94803202454576 12-13 25.403485810579202 24.614252463582602 28.377523209572914 21.60473851626528 14-15 22.160415862797873 25.812037301109882 27.306334526054936 24.721212310037316 16-17 20.797943420082817 29.15094170027385 28.138351739883387 21.912763139759946 18-19 20.77438713125421 27.058134156483142 31.50309519986387 20.664383512398775 20-21 21.376571489516213 26.18196503447061 31.786539864993607 20.65492361101957 22-23 21.03613854326571 26.092922204526637 31.777879237803525 21.093060014404127 24-25 20.790876237766128 26.35039924847346 31.93784089493007 20.92088361883034 26-27 20.440534969560424 26.644456162216578 31.679210009107905 21.235798859115096 28-29 20.53277824115352 26.85570000095871 31.759253933101327 20.85226782478645 30-31 20.596229671264727 26.6581177119162 31.509809326350357 21.235843290468715 32-33 20.254153697908738 26.63388372402403 31.531789586721594 21.580172991345638 34-35 20.966487509961475 26.69614663287256 31.305686877220733 21.031678979945234 36-37 20.98364506140292 26.343218835980863 31.425017495757878 21.248118606858338 38-39 20.511354990072697 26.852008286932026 31.33121726595947 21.305419457035804 40-41 20.871127066024105 26.765152041640405 31.367140601073125 20.99658029126237 42-43 20.60629661803912 26.8329389210077 31.039151664020608 21.521612796932573 44-45 20.66783953443222 27.082938848688077 31.085709628940748 21.163511987938957 46-47 21.098261393689057 27.33059403081296 30.269652338652563 21.301492236845416 48-49 20.84707495254348 27.464862999252198 30.01720898126666 21.670853066937664 50-51 21.10614675116732 27.659301623217864 29.894941466361136 21.339610159253684 52-53 21.16737141870901 27.674581943006178 29.814607064779658 21.343439573505158 54-55 20.888046714320645 27.620249584106393 30.00079103683355 21.490912664739415 56-57 21.03158199949438 27.918061012124067 29.613321495624962 21.437035492756593 58-59 20.951537775903546 27.564836734107097 29.718513690949695 21.76511179903967 60-61 20.593792496121058 27.691026616425145 30.079136388960443 21.636044498493355 62-63 20.74717780488214 27.46557173563502 30.320715427360973 21.466535032121865 64-65 20.56078461442613 27.531474195233464 30.189348375131193 21.718392815209214 66-67 20.348207438650306 27.664637653374236 30.011023773006134 21.976131134969325 68-69 20.795816034938262 27.38747805874565 30.07386639348682 21.742839512829267 70-71 20.831356366790796 27.38482073107025 29.71620345741494 22.067619444724016 72-73 20.59597506524881 27.362078758726803 29.76819596931329 22.273750206711103 74-75 20.635238241298993 27.669610934849082 29.56832842888226 22.126822394969665 76-77 20.785855087746626 27.422244324479784 29.579451291484173 22.212449296289417 78-79 20.66529660940786 27.315266951462824 29.77573800351102 22.243698435618292 80-81 20.75261911836594 27.419149996645224 29.617843552607614 22.210387332381217 82-83 20.774785440897688 27.29532104177102 29.64103141754004 22.288862099791253 84-85 20.55839175124334 27.40032612589514 29.554050994013036 22.487231128848485 86-87 20.730317760234264 27.30214719699793 29.519315906702786 22.448219136065024 88-89 20.78821523445036 27.141913969965337 29.33657971959179 22.73329107599252 90-91 20.412237229321036 27.117502707987846 29.69380565753779 22.776454405153324 92-93 20.548841422645282 27.03862403811359 29.671155270214893 22.741379269026236 94-95 20.45011191686997 27.037390299898867 29.510585370763575 23.001912412467586 96-97 20.4944460486131 26.838087729787176 29.42174445952762 23.2457217620721 98-99 20.6267763936752 27.33645195768768 28.94509176136772 23.091679887269397 100-101 20.91766684797324 27.152017175403326 28.776587970029304 23.15372800659413 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 581.0 1 352.5 2 157.0 3 184.5 4 152.5 5 135.5 6 216.5 7 510.5 8 878.0 9 1007.5 10 875.5 11 735.5 12 688.0 13 683.0 14 723.0 15 756.5 16 810.5 17 843.0 18 902.0 19 997.5 20 1136.5 21 1347.5 22 1587.0 23 1870.5 24 2243.5 25 2711.0 26 3432.0 27 4283.0 28 4970.5 29 5683.0 30 6397.5 31 7328.5 32 8735.0 33 9487.5 34 9823.0 35 10449.5 36 11506.5 37 12360.5 38 12556.5 39 12890.0 40 13225.0 41 13598.0 42 13723.0 43 13774.0 44 14172.5 45 14571.5 46 14752.5 47 15046.0 48 15457.5 49 15043.0 50 14099.0 51 13350.5 52 12711.0 53 12339.5 54 12122.5 55 11077.0 56 9720.0 57 8685.5 58 7929.5 59 6885.0 60 5379.0 61 4527.5 62 3797.0 63 2626.0 64 1693.0 65 1286.5 66 943.0 67 707.0 68 571.0 69 447.5 70 342.5 71 227.5 72 149.0 73 100.5 74 78.5 75 63.0 76 45.0 77 32.0 78 23.5 79 13.5 80 6.5 81 5.5 82 2.0 83 1.0 84 2.0 85 3.0 86 2.0 87 0.5 88 0.0 89 1.5 90 2.0 91 0.5 92 0.5 93 0.5 94 1.0 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3123908665138997 2 0.2777073476436735 3 0.28129529787162794 4 0.280577707826037 5 0.2688570704147192 6 0.19829404926494856 7 0.20786191653949376 8 0.20570914640272112 9 0.20259958953849394 10-11 0.21192826013117544 12-13 0.21264585017676635 14-15 0.19446690235513053 16-17 0.20774231819856193 18-19 0.19303172226394877 20-21 0.19769605756028952 22-23 0.19721766419656225 24-25 0.187051805217358 26-27 0.2023603928566303 28-29 0.20044681940172127 30-31 0.20594834308458473 32-33 0.20989508833533463 34-35 0.19937043433333493 36-37 0.19661967249190318 38-39 0.18717140355828984 40-41 0.18633421517176715 42-43 0.18621461683083532 44-45 0.20451316299340294 46-47 0.19291212392301693 48-49 0.20140360612917577 50-51 0.2081011132213574 52-53 0.21479862031353902 54-55 0.21336344022235723 56-57 0.18071309314797185 58-59 0.16982964412317672 60-61 0.17903871637492644 62-63 0.17915831471585827 64-65 0.1770055445790856 66-67 0.18800859194481254 68-69 0.18143068319356273 70-71 0.18178947821635819 72-73 0.19542368908258503 74-75 0.18131108485263092 76-77 0.19458650069606234 78-79 0.1933905172867442 80-81 0.1793975113977219 82-83 0.1949452957188578 84-85 0.17760353628374467 86-87 0.18047389646610823 88-89 0.18693220687642623 90-91 0.187051805217358 92-93 0.18824778862667615 94-95 0.18944377203599433 96-97 0.1795171097386537 98-99 0.1884869853085398 100-101 0.17341759435113116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 418066.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.45079806502827 #Duplication Level Percentage of deduplicated Percentage of total 1 87.81383661302672 63.621825437657854 2 7.702201006356181 11.160612195355382 3 1.875804617076008 4.077106245636646 4 0.8009456638228789 2.321166102027657 5 0.44955681834833017 1.6285375132455733 6 0.2896563697155351 1.2591501090305641 7 0.19791928272677645 1.0037586990209042 8 0.14240434705705238 0.8253846873770163 9 0.10614222711621026 0.6921080156672024 >10 0.5693175500454482 7.303590403737334 >50 0.028421097541992443 1.39350506610758 >100 0.02214201778024953 2.914388938377424 >500 6.609557546343144E-4 0.26816592269000283 >1k 9.914336319514717E-4 1.5307006640689182 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2801 0.6699899059000254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2109 0.5044658020503939 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1483 0.3547286792037621 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 563 0.13466773188922324 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 557 0.13323255179804144 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.3919668186362918E-4 14-15 0.0 0.0 0.0 0.0 2.3919668186362918E-4 16-17 0.0 0.0 0.0 0.0 2.3919668186362918E-4 18-19 0.0 0.0 0.0 0.0 4.7839336372725835E-4 20-21 0.0 0.0 0.0 0.0 4.7839336372725835E-4 22-23 0.0 0.0 0.0 2.3919668186362918E-4 4.7839336372725835E-4 24-25 0.0 0.0 0.0 2.3919668186362918E-4 4.7839336372725835E-4 26-27 0.0 0.0 0.0 5.979917046590729E-4 4.7839336372725835E-4 28-29 0.0 0.0 0.0 0.0033487535460908083 4.7839336372725835E-4 30-31 0.0 0.0 0.0 0.009089473910817908 4.7839336372725835E-4 32-33 0.0 0.0 0.0 0.01973372625374941 4.7839336372725835E-4 34-35 0.0 0.0 0.0 0.03097597030133998 4.7839336372725835E-4 36-37 0.0 0.0 0.0 0.04652375462247588 4.7839336372725835E-4 38-39 0.0 0.0 0.0 0.07319418465027053 4.7839336372725835E-4 40-41 0.0 0.0 0.0 0.10692091679304225 4.7839336372725835E-4 42-43 0.0 0.0 0.0 0.15834820339372252 4.7839336372725835E-4 44-45 0.0 0.0 0.0 0.22735644611137953 5.979917046590729E-4 46-47 0.0 0.0 0.0 0.3084441212631498 7.175900455908876E-4 48-49 0.0 0.0 0.0 0.40591676912257874 7.175900455908876E-4 50-51 0.0 0.0 0.0 0.5294618553051432 7.175900455908876E-4 52-53 0.0 0.0 0.0 0.652169753101185 7.175900455908876E-4 54-55 0.0 0.0 0.0 0.782412346375931 7.175900455908876E-4 56-57 0.0 0.0 0.0 0.9703013399798117 7.175900455908876E-4 58-59 0.0 0.0 0.0 1.1879703204757144 7.175900455908876E-4 60-61 0.0 0.0 0.0 1.4266886089756163 7.175900455908876E-4 62-63 0.0 0.0 0.0 1.7014059980959946 8.371883865227021E-4 64-65 0.0 0.0 0.0 1.9749274038070546 9.567867274545167E-4 66-67 0.0 0.0 0.0 2.3123143235757038 9.567867274545167E-4 68-69 0.0 0.0 0.0 2.7127295690154183 9.567867274545167E-4 70-71 0.0 0.0 0.0 3.1886113675831087 9.567867274545167E-4 72-73 0.0 0.0 0.0 3.724053139934843 9.567867274545167E-4 74-75 0.0 0.0 0.0 4.257342142149804 9.567867274545167E-4 76-77 0.0 0.0 0.0 4.834284538804877 9.567867274545167E-4 78-79 0.0 0.0 0.0 5.537761980165811 9.567867274545167E-4 80-81 0.0 0.0 0.0 6.359641779049241 9.567867274545167E-4 82-83 0.0 0.0 0.0 7.173030095726512 9.567867274545167E-4 84-85 0.0 0.0 0.0 8.04753316461994 9.567867274545167E-4 86-87 0.0 0.0 0.0 9.026445585146842 0.0011959834093181459 88-89 0.0 0.0 0.0 10.098046719895901 0.0011959834093181459 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1270 0.0 46.05179 1 GTATCAA 2225 0.0 39.53546 1 ATCAACG 2810 0.0 30.962622 3 TCAACGC 2810 0.0 30.962622 4 TATCAAC 2815 0.0 30.907625 2 CAACGCA 2865 0.0 30.202278 5 AACGCAG 2950 0.0 29.307415 6 ATGGGAG 460 0.0 27.906084 5 ACGCAGA 3140 0.0 27.685322 7 CGCAGAG 3185 0.0 27.145016 8 GTACATG 1740 0.0 26.234154 1 GCAGAGT 3360 0.0 25.4454 9 TACATGG 1725 0.0 25.35664 2 GAGTACT 2005 0.0 25.348103 12-13 AGTACTT 2090 0.0 24.20357 12-13 ACATGGG 1755 0.0 24.110477 3 GTACTTT 2155 0.0 23.798435 14-15 TAAGGGT 100 0.0016430642 23.77185 4 ATACTGC 125 2.251342E-4 22.801815 6 ATTACAC 210 3.3995093E-8 22.639856 3 >>END_MODULE