FastQCFastQC Report
Thu 26 May 2016
SRR937165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418066
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26310.6293264699832084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22990.5499131716044835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.3764955772533523No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC7780.1860950184899035No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.1502155162103591No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA6200.1483019427554501No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA5430.12988379825195065No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA4940.11816316084063282No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA4900.1172063741131783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.10046260638272425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.042.4133031
GTATCAA24700.033.861461
TAAGGTG3150.031.6696175
AAGGTAA2850.030.0135651
GTAAGGT3050.029.592924
AGGTAAG2900.029.4855042
GGTAAGG2900.029.4855043
TATCAAC31350.026.5176222
TCAACGC31350.026.5176224
ATCAACG31250.026.4504623
CAACGCA31950.026.0196385
GGTGTGC4050.025.8017868
CCTATAC950.00122458425.0023293
AACGCAG33300.024.9618036
TATAGGG1652.4217788E-623.0324482
ACGCAGA37600.021.9808037
AAGGTGT4350.021.8385036
CGCAGAG38150.021.5394068
CCCTAAG1553.8852028E-521.4613131
GTGTGCA4900.021.3259689
Produced by FastQC (version 0.11.5)