##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937165_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 418066 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806123913449074 31.0 31.0 34.0 30.0 34.0 2 32.05591700831926 31.0 31.0 34.0 30.0 34.0 3 32.11240091277454 33.0 31.0 34.0 30.0 34.0 4 35.84599082441528 37.0 35.0 37.0 35.0 37.0 5 35.6964402749805 37.0 35.0 37.0 33.0 37.0 6 35.795369630632486 37.0 35.0 37.0 35.0 37.0 7 35.74165083981955 37.0 35.0 37.0 33.0 37.0 8 35.70707735142298 37.0 35.0 37.0 33.0 37.0 9 37.34774174412652 39.0 37.0 39.0 34.0 39.0 10-11 37.24946300344922 39.0 37.0 39.0 33.5 39.0 12-13 37.1631716044835 39.0 37.0 39.0 33.0 39.0 14-15 38.23749599345558 40.0 38.0 41.0 33.0 41.0 16-17 38.1998536116307 40.0 37.5 41.0 33.0 41.0 18-19 38.236357417249906 40.0 38.0 41.0 33.5 41.0 20-21 38.120065970444855 40.0 37.5 41.0 33.0 41.0 22-23 37.981396478068056 40.0 37.0 41.0 33.0 41.0 24-25 37.819669621543014 39.5 37.0 41.0 33.0 41.0 26-27 37.717279089904466 39.0 37.0 41.0 32.5 41.0 28-29 37.61255399865093 39.0 37.0 41.0 32.0 41.0 30-31 37.646104921232535 39.0 37.0 41.0 33.0 41.0 32-33 37.53297445857831 39.0 36.0 41.0 33.0 41.0 34-35 37.37266005845967 39.0 36.0 41.0 32.0 41.0 36-37 37.14804959025608 39.0 36.0 41.0 31.5 41.0 38-39 36.971329885711825 39.0 35.5 41.0 31.0 41.0 40-41 36.76424775035521 39.0 35.0 40.5 31.0 41.0 42-43 36.52737247228906 39.0 35.0 40.0 30.5 41.0 44-45 36.16394660173274 39.0 35.0 40.0 30.0 41.0 46-47 35.744193500547766 38.5 35.0 40.0 28.5 41.0 48-49 35.36665980969512 38.0 35.0 40.0 27.0 41.0 50-51 35.08949185056905 38.0 34.5 40.0 26.5 41.0 52-53 34.81021896064258 38.0 34.0 40.0 26.0 41.0 54-55 34.534762453775244 38.0 34.0 40.0 24.5 41.0 56-57 34.24539665985753 38.0 34.0 40.0 24.0 41.0 58-59 33.95189276334359 37.5 33.0 40.0 23.0 41.0 60-61 33.5825671066291 37.0 33.0 40.0 22.5 41.0 62-63 33.15879191323858 36.5 32.5 39.0 21.5 41.0 64-65 32.76275994699402 36.0 32.0 39.0 20.0 40.0 66-67 32.31669162285381 35.5 31.0 39.0 18.5 40.0 68-69 31.860435912033026 35.0 31.0 38.0 17.5 40.0 70-71 31.41300057885597 35.0 30.5 38.0 13.5 40.0 72-73 30.933498538508275 34.5 30.0 37.0 10.0 39.0 74-75 30.4518305722063 34.0 29.5 36.5 7.5 39.0 76-77 29.233186865231808 33.0 28.5 35.0 7.0 37.5 78-79 29.902955514201107 34.0 29.0 36.0 6.5 38.0 80-81 29.751437572058002 34.0 29.0 35.5 2.0 37.0 82-83 29.39380982907005 34.0 29.0 35.0 2.0 37.0 84-85 28.978278549319967 34.0 29.0 35.0 2.0 36.5 86-87 28.516371816890157 34.0 29.0 35.0 2.0 36.0 88-89 28.022639965938392 33.0 27.5 35.0 2.0 35.5 90-91 27.497714475704793 33.0 26.5 35.0 2.0 35.0 92-93 27.020070993575175 32.5 25.5 34.5 2.0 35.0 94-95 26.46736161275971 32.0 25.0 34.0 2.0 35.0 96-97 25.82838714461353 32.0 24.0 34.0 2.0 35.0 98-99 25.11727454516751 32.0 21.0 34.0 2.0 35.0 100-101 23.082638865633655 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2690533440432539 1101 2 0.28818634498937 1101 3 0.2195387588295219 1101 4 0.1441013294577118 1101 5 0.060282676309114436 1101 6 0.07527038822475163 1101 7 0.16880393844517982 1101 8 0.0667225726198808 1101 9 0.21988909777833499 1101 10-11 0.006712737767209376 1101 12-13 0.0812256746080493 1101 14-15 0.14195358646779965 1101 16-17 0.25322449355936527 1101 18-19 0.19088339706859614 1101 20-21 0.07906154873277416 1101 22-23 0.015466157289829141 1101 24-25 0.11465077484742636 1101 26-27 -0.15873375751541374 1101 28-29 -0.0028894552136762286 1101 30-31 0.07479592312849093 1101 32-33 -0.01673418626881329 1101 34-35 0.04619338729381184 1101 36-37 0.06382384733547752 1101 38-39 -0.1342907595503675 1101 40-41 -0.30224712717470226 1101 42-43 -0.1588318111236262 1101 44-45 -0.11275032177925937 1101 46-47 0.13432874172661258 1101 48-49 0.25164430748120026 1101 50-51 0.24407985958584533 1101 52-53 0.0037710423037893293 1101 54-55 0.08717510890831903 1101 56-57 0.26777112663290836 1101 58-59 0.2519551687414463 1101 60-61 0.32157319888055724 1101 62-63 0.33496618780282006 1101 64-65 0.2812802057238315 1101 66-67 0.6280407574918101 1101 68-69 0.34944987264631777 1101 70-71 0.08539961115249639 1101 72-73 0.16649276775042665 1101 74-75 0.0760152790448494 1101 76-77 0.05852361054494892 1101 78-79 0.24213094382780298 1101 80-81 0.27604622344251695 1101 82-83 0.23876281919796583 1101 84-85 0.18621913129065248 1101 86-87 0.18362944069435017 1101 88-89 0.06495957203763325 1101 90-91 0.3955510050404172 1101 92-93 0.5125692326731901 1101 94-95 0.35622922052689887 1101 96-97 0.20949444549329144 1101 98-99 0.45210858299180146 1101 100-101 0.8503395796744613 1102 1 0.11655975702344179 1102 2 0.07289433800197287 1102 3 0.25449618923098427 1102 4 0.07230742611667296 1102 5 0.012395418861402163 1102 6 0.12595732463002207 1102 7 -0.002587088103773283 1102 8 0.03202795637576372 1102 9 0.11957120280957412 1102 10-11 -0.004857380105725895 1102 12-13 0.005076452821228372 1102 14-15 0.0027777527803749535 1102 16-17 -0.04606339023585804 1102 18-19 0.16441785405048392 1102 20-21 0.04704543366108993 1102 22-23 0.056787782016392896 1102 24-25 0.03804516919455381 1102 26-27 0.029537963996006056 1102 28-29 -0.14785270844377152 1102 30-31 -0.014874850992967481 1102 32-33 0.22486756372725125 1102 34-35 0.05311232407677835 1102 36-37 0.016971322273711564 1102 38-39 0.0022317527641249058 1102 40-41 0.21036244429658524 1102 42-43 0.23253938633321525 1102 44-45 0.4542964024862073 1102 46-47 0.38761942677454186 1102 48-49 0.3475864254170844 1102 50-51 0.15902170998636223 1102 52-53 0.2099440275263902 1102 54-55 0.21205039021389638 1102 56-57 0.3750496624275712 1102 58-59 0.19791538465822356 1102 60-61 0.36176015889189017 1102 62-63 0.264653958288946 1102 64-65 0.1565339708907274 1102 66-67 0.04468067062150993 1102 68-69 0.20724695493704814 1102 70-71 0.4247957680855947 1102 72-73 0.20142973956479082 1102 74-75 0.2986967703184753 1102 76-77 0.16550785743288188 1102 78-79 0.37214290423256813 1102 80-81 0.25181460076181494 1102 82-83 -0.1744786078671723 1102 84-85 0.025252541737120993 1102 86-87 0.16890319232710382 1102 88-89 -0.06397516506951817 1102 90-91 -0.015159852174377875 1102 92-93 0.20656857170345333 1102 94-95 0.1585953348117144 1102 96-97 0.09087319688998363 1102 98-99 0.22227838926471932 1102 100-101 0.7652735613876338 1103 1 0.06946261491829375 1103 2 0.05588953463303881 1103 3 -0.22847049425113042 1103 4 -0.0028269146812576196 1103 5 -0.0852665029248385 1103 6 -0.085092617247021 1103 7 -0.03360720084037894 1103 8 -0.11631937764481393 1103 9 -0.11285702082124516 1103 10-11 -0.23473038692694814 1103 12-13 -0.12920183551250375 1103 14-15 -0.35693426441525133 1103 16-17 -0.0692676101819032 1103 18-19 -0.11060243415165871 1103 20-21 -0.22379000160309204 1103 22-23 -0.11008213222483221 1103 24-25 -0.21043486063458516 1103 26-27 -0.004367460082249863 1103 28-29 -0.24054237571726134 1103 30-31 -0.1683688083987107 1103 32-33 -0.04783877277177595 1103 34-35 0.11911478059423075 1103 36-37 0.09768656364848027 1103 38-39 0.3561358608508911 1103 40-41 0.1909153800542711 1103 42-43 0.1559508749389238 1103 44-45 -0.08674827542488117 1103 46-47 0.12162355021453664 1103 48-49 0.6128080603171782 1103 50-51 0.46895196243739434 1103 52-53 0.1954165781372339 1103 54-55 0.39188172397127374 1103 56-57 0.19666920928783327 1103 58-59 0.32835536447969105 1103 60-61 0.2761307859618327 1103 62-63 0.06898905358866614 1103 64-65 0.013083221872236095 1103 66-67 0.24349518162100736 1103 68-69 0.4290441606484521 1103 70-71 0.17333501753178382 1103 72-73 0.4907153755176772 1103 74-75 0.6175384784754279 1103 76-77 0.3760555292246046 1103 78-79 0.1669897008899497 1103 80-81 0.2996015417395945 1103 82-83 0.2477133952912105 1103 84-85 0.22220382811880413 1103 86-87 0.09596984729607172 1103 88-89 0.19705174695645056 1103 90-91 0.2888736617422296 1103 92-93 0.30266665693583406 1103 94-95 0.5940689820821277 1103 96-97 0.420913992626172 1103 98-99 0.1473085474030249 1103 100-101 -0.15921155694193345 1104 1 -0.04857995397934545 1104 2 0.06881399329445514 1104 3 -0.14036148834561857 1104 4 0.10140844457070841 1104 5 0.004555716563352519 1104 6 0.02365492212305753 1104 7 -0.21511864276951087 1104 8 -0.08036649870780366 1104 9 -0.10324826097872375 1104 10-11 -0.2303889130096266 1104 12-13 -0.07671813718573617 1104 14-15 0.060808415210736655 1104 16-17 -0.022598687937808393 1104 18-19 -0.15613946663196998 1104 20-21 -0.22715645091411574 1104 22-23 -0.20228962484294044 1104 24-25 -0.017858273528283064 1104 26-27 -0.09027727799563934 1104 28-29 -0.10640728713458003 1104 30-31 -0.2771594087924143 1104 32-33 -0.15140822405130194 1104 34-35 0.08891054831076417 1104 36-37 -0.17132026459955085 1104 38-39 -0.3561150003695772 1104 40-41 -0.13711427053628 1104 42-43 0.15923735377750603 1104 44-45 0.10839198048063281 1104 46-47 -0.07670475933270637 1104 48-49 -0.2541256872221922 1104 50-51 -0.6191109066570917 1104 52-53 -0.664922681213163 1104 54-55 -0.5632640683515646 1104 56-57 -0.18091906581059192 1104 58-59 -0.4557966409336984 1104 60-61 -0.7830811973058829 1104 62-63 -0.7847018912932242 1104 64-65 -1.0463978927931166 1104 66-67 -0.7477603547963199 1104 68-69 -0.7011493063791079 1104 70-71 -0.9492766335508946 1104 72-73 -1.1853842184783865 1104 74-75 -1.0329536475088226 1104 76-77 -0.9696168379013805 1104 78-79 -0.6666040491100489 1104 80-81 -0.3921045975320574 1104 82-83 -0.35204976907887087 1104 84-85 -0.21247106115285064 1104 86-87 -0.31531675457400965 1104 88-89 -0.2533358022561494 1104 90-91 -0.3138791679821793 1104 92-93 -0.10624697495392965 1104 94-95 0.2762724761766151 1104 96-97 0.504715358275778 1104 98-99 0.4005350483872796 1104 100-101 0.7162713416801125 1105 1 -0.14134674819703363 1105 2 -0.1700518655490839 1105 3 0.01070675145030009 1105 4 -0.07940841342249172 1105 5 -0.022376149933243994 1105 6 0.09513663981013565 1105 7 -0.30210578137495503 1105 8 -0.2970039349322917 1105 9 -0.35096297186307623 1105 10-11 -0.07647070510146392 1105 12-13 -0.0954721231551261 1105 14-15 -0.3615515443981039 1105 16-17 -0.23067957654325255 1105 18-19 -0.17063510863877696 1105 20-21 -0.6681308875637768 1105 22-23 -0.5651922077511031 1105 24-25 -0.4992371532051507 1105 26-27 -0.5317765339480189 1105 28-29 -0.390978928291041 1105 30-31 -0.6241779036903736 1105 32-33 -0.5402727542383801 1105 34-35 -0.10246912855998858 1105 36-37 0.15647921456711344 1105 38-39 0.2961888899365448 1105 40-41 -0.23701594995804953 1105 42-43 -0.4085839153551447 1105 44-45 -0.07342136985950276 1105 46-47 -0.5026653779551609 1105 48-49 -0.5783510805045111 1105 50-51 -0.759217730636685 1105 52-53 -0.7081106595294884 1105 54-55 -0.6922898974331915 1105 56-57 -0.856228642766375 1105 58-59 -0.835108131154783 1105 60-61 -0.5142275621018015 1105 62-63 -0.3214791021775767 1105 64-65 -0.06257428522508945 1105 66-67 -0.4865332076874793 1105 68-69 -0.3508556733008774 1105 70-71 -0.6394937360168811 1105 72-73 -2.0272400560017445E-4 1105 74-75 -0.012120314175490421 1105 76-77 -0.3478986193639635 1105 78-79 -0.10205791815766929 1105 80-81 -0.4760291829912404 1105 82-83 -0.3706110998611827 1105 84-85 -0.6853996857276776 1105 86-87 -0.15670838935292153 1105 88-89 -0.07330285157587824 1105 90-91 -0.2859375225740166 1105 92-93 -0.8294346875389635 1105 94-95 -0.8022511207621577 1105 96-97 -0.9322731089861271 1105 98-99 -0.8567061293338085 1105 100-101 -0.4912780843402942 1106 1 -0.5257004896936301 1106 2 -0.30932862888599644 1106 3 -0.16705909172907596 1106 4 0.0013291447586780691 1106 5 -0.2508043633238657 1106 6 -0.4646556981776939 1106 7 -0.5140642489725167 1106 8 -0.352320798519834 1106 9 -0.21526658804638998 1106 10-11 -0.6006382668630081 1106 12-13 -0.6344087504376148 1106 14-15 -0.5405705204095668 1106 16-17 -0.11369067571975933 1106 18-19 -0.36979371945152195 1106 20-21 -0.4199210773238917 1106 22-23 -0.25719005952338847 1106 24-25 -0.04202268847189572 1106 26-27 -0.17901964171744567 1106 28-29 -0.4584690822473618 1106 30-31 -0.21386641067211087 1106 32-33 -0.33950297264152596 1106 34-35 -0.5947713125914902 1106 36-37 -0.3767288054830047 1106 38-39 -0.5762600896552854 1106 40-41 0.14259378867424033 1106 42-43 -0.4021392322187367 1106 44-45 -0.20616394057199727 1106 46-47 -0.2886481921334578 1106 48-49 0.07104025497705635 1106 50-51 -0.10159152225859458 1106 52-53 -0.23120410743496933 1106 54-55 -0.11978721214855881 1106 56-57 -0.10587060481434918 1106 58-59 0.18377837081442294 1106 60-61 0.24812474724298994 1106 62-63 0.2108338229924911 1106 64-65 -0.0014393049171772532 1106 66-67 -0.41277738957075627 1106 68-69 -0.05019330308963532 1106 70-71 -0.07039241127645823 1106 72-73 0.23699741920958317 1106 74-75 -0.15560402344867086 1106 76-77 -0.048578891472811137 1106 78-79 0.013230302393711924 1106 80-81 -0.20776924743807257 1106 82-83 -0.2230994636277437 1106 84-85 -0.616745908427287 1106 86-87 -0.4913843650063434 1106 88-89 -0.40180267255690083 1106 90-91 -0.4473231294483426 1106 92-93 -0.3029398790892692 1106 94-95 -1.1056882851015786 1106 96-97 -0.11773676813399803 1106 98-99 0.28812022225946166 1106 100-101 0.8078626245748488 1107 1 -0.1942345699924175 1107 2 -0.16298237108463098 1107 3 -0.24332819586195953 1107 4 0.0030245597667857282 1107 5 -0.4092642385020042 1107 6 -0.23926378702726936 1107 7 -0.08962078737735624 1107 8 -0.10552734429603561 1107 9 -0.06071353875650232 1107 10-11 -0.0373550588429552 1107 12-13 -0.16406622746351474 1107 14-15 -0.4174072200016852 1107 16-17 -0.35642987666329873 1107 18-19 -0.4050288661417767 1107 20-21 -0.411394859819346 1107 22-23 -0.06019020695078581 1107 24-25 -0.1817101424008314 1107 26-27 -0.2357914732570805 1107 28-29 -0.4356301221019052 1107 30-31 -0.6442192535636551 1107 32-33 -0.4460684058323423 1107 34-35 -0.5182687817545997 1107 36-37 -0.11312862857013783 1107 38-39 -0.36034038844147176 1107 40-41 -0.4001478693924909 1107 42-43 -0.5666804873172637 1107 44-45 -0.28330532344094195 1107 46-47 -0.39005366660394714 1107 48-49 -0.36043057897055775 1107 50-51 0.06161060069585034 1107 52-53 -0.26183214812493105 1107 54-55 -0.4674332881228125 1107 56-57 0.18448764374823412 1107 58-59 -0.07627126307421861 1107 60-61 -0.18829718995294087 1107 62-63 -0.05900744684878134 1107 64-65 0.18495806104674983 1107 66-67 0.11017214111871709 1107 68-69 -0.08261504371571249 1107 70-71 -0.42504128837115474 1107 72-73 -0.41163396316792955 1107 74-75 -0.18695691548267845 1107 76-77 -0.025236221071313736 1107 78-79 -0.009154089959722 1107 80-81 0.22281702215748567 1107 82-83 0.1348911010191678 1107 84-85 0.19541930853669243 1107 86-87 -0.19985898292368276 1107 88-89 -0.2695480163084376 1107 90-91 -0.3301552096488436 1107 92-93 -0.37945469554216515 1107 94-95 -0.3103543620586784 1107 96-97 -0.6500602238320639 1107 98-99 -0.5129019409918065 1107 100-101 0.39867789805266085 1108 1 -0.21160192450336268 1108 2 -0.16663565190205176 1108 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-0.0991977651838134 2316 94-95 0.2540472606593731 2316 96-97 -0.089771740622691 2316 98-99 0.308768106337471 2316 100-101 -0.46502505439268305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 9.0 5 11.0 6 27.0 7 67.0 8 146.0 9 315.0 10 514.0 11 783.0 12 1215.0 13 1630.0 14 2223.0 15 3011.0 16 3979.0 17 5414.0 18 4774.0 19 3277.0 20 2839.0 21 2775.0 22 2889.0 23 3253.0 24 3700.0 25 4416.0 26 5277.0 27 6469.0 28 7915.0 29 9618.0 30 12081.0 31 14894.0 32 18473.0 33 23972.0 34 32086.0 35 44651.0 36 63066.0 37 80291.0 38 48942.0 39 3062.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.695105082539214 21.13281418267275 16.211811555010986 20.960269179777054 2 16.223754144082513 21.576258294144942 39.47367162122727 22.726315940545273 3 19.587577081130732 25.63088124841532 30.952529026517343 23.829012643936604 4 13.21346390282874 18.441107384958354 37.405098716470604 30.9403299957423 5 12.938347807363812 38.58393741477069 34.30560539725831 14.172109380607193 6 31.003477919754296 38.08465648964518 16.822463438787178 14.08940215181335 7 27.239957327311952 34.29841221242579 20.703429602024563 17.758200858237693 8 26.442475589978613 35.6998655714648 19.343357268947965 18.514301569608627 9 26.148981261331944 17.098018016294077 20.45562183961384 36.29737888276014 10-11 24.5353604454799 27.817258518989824 27.12023938803921 20.527141647491064 12-13 25.788530723156704 25.208225692251755 27.758123313623056 21.24512027096848 14-15 22.447771404515077 26.402290547425526 26.74864734276406 24.401290705295338 16-17 20.91882621404276 29.799720618275582 27.686059138987623 21.595394028694034 18-19 20.828409868298305 27.595044801538513 31.13360091468811 20.44294441547507 20-21 21.632708711064762 26.512201422741867 31.71293527816182 20.142154588031556 22-23 21.254905056289296 26.55290404448128 31.553579779148382 20.63861112008104 24-25 21.39105163072728 26.81616495042637 31.320727638046712 20.472055780799636 26-27 20.807749071312216 27.291341257178942 31.28882489002854 20.612084781480302 28-29 20.708345841550884 27.513493924893282 31.36977241223417 20.408387821321657 30-31 20.98245253141848 27.093808154693278 31.247817330277993 20.675921983610245 32-33 20.797194701315103 27.134591188951028 30.99773719938957 21.0704769103443 34-35 21.233728645716226 27.254907119928433 30.95025665803964 20.561107576315703 36-37 21.57482311405376 26.837867705099193 30.836757832495348 20.750551348351696 38-39 21.095712160280915 27.22452914133175 30.99678041266212 20.68297828572522 40-41 21.309434395526065 27.199413489736067 30.97250194945296 20.518650165284907 42-43 21.113050218358893 27.33248087042125 30.62060336671017 20.93386554450969 44-45 21.106236814282912 27.683786770509922 30.471504499289587 20.738471915917582 46-47 21.811508230757823 27.910186429893848 29.558610362957044 20.71969497639129 48-49 21.60687546942349 28.260729167164993 29.160228289313174 20.972167074098348 50-51 21.589772906670238 28.413456248534917 29.292623652724693 20.704147192070153 52-53 21.799787593346505 28.172226394875448 29.25913611726378 20.76884989451426 54-55 21.57470351571283 28.35078671788665 29.323360426344163 20.751149340056354 56-57 21.703032535532667 28.541546071672897 28.905483823128403 20.849937569666032 58-59 21.343041529327905 28.182631450536515 29.14252773485526 21.331799285280315 60-61 21.12776451565064 28.370998837504125 29.40552448656432 21.095712160280915 62-63 21.29532179129611 28.2998378246497 29.617333148354568 20.787507235699625 64-65 21.10659560930571 28.473255419000825 29.302191519999234 21.11795745169423 66-67 20.95566250304976 28.376619959527922 29.19574899656992 21.4719685408524 68-69 21.290298660976976 28.187654580855654 29.19838016007042 21.323666598096953 70-71 21.43297948170863 28.27508096807681 28.846761037730882 21.445178512483675 72-73 21.352728994943384 28.003592734161593 28.91313811694804 21.730540153946983 74-75 21.1595776743385 28.279386508350356 28.725727516707888 21.835308300603256 76-77 21.140322341448478 28.11410160118259 28.93095826974688 21.81461778762205 78-79 21.129438892423686 28.315026813948034 28.713887280955642 21.84164701267264 80-81 21.15240177388259 28.341338448953035 28.762205010692092 21.744054766472278 82-83 21.13468272800337 28.3341784989858 28.702423552374757 21.828715220636074 84-85 20.951846740069033 28.298860692578355 28.696769131777423 22.052523435575193 86-87 21.064855788320504 28.241713030956834 28.43390756483426 22.259523615888398 88-89 20.887969842082352 27.953720225993024 28.486889629867058 22.67142030205757 90-91 20.678943679324046 28.293346921297456 28.483650186475668 22.544059212902827 92-93 20.733865952255655 28.016433047887862 28.80555422666603 22.44414677319045 94-95 20.402639774581047 28.080374869039815 28.512124879803668 23.00486047657547 96-97 20.53551353135629 28.161462544191586 28.25080250486765 23.052221419584466 98-99 20.312057110804673 28.503735667582397 27.992809721944816 23.191397499668113 100-101 20.5085388449563 28.528127291409238 27.99547370045323 22.967860163181232 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 237.0 1 171.5 2 143.0 3 195.5 4 164.5 5 142.0 6 230.5 7 445.0 8 712.0 9 845.0 10 767.0 11 692.5 12 726.0 13 750.0 14 797.0 15 840.5 16 866.5 17 899.0 18 941.0 19 990.5 20 1097.5 21 1333.0 22 1564.0 23 1859.0 24 2176.5 25 2601.0 26 3362.0 27 4251.0 28 5000.0 29 5722.0 30 6617.0 31 7581.0 32 8780.0 33 9484.0 34 9781.0 35 10453.5 36 11585.5 37 12368.0 38 12690.0 39 12967.0 40 13209.5 41 13891.5 42 13968.0 43 13828.5 44 14375.5 45 14654.0 46 14571.5 47 14811.5 48 15306.5 49 15032.5 50 14079.5 51 13255.5 52 12552.5 53 12291.5 54 12302.0 55 11270.5 56 9697.0 57 8662.5 58 8068.5 59 6940.0 60 5311.5 61 4452.5 62 3744.0 63 2619.0 64 1668.5 65 1152.5 66 839.5 67 673.0 68 564.5 69 455.0 70 352.0 71 230.0 72 152.5 73 116.5 74 78.5 75 54.5 76 36.5 77 25.5 78 17.0 79 13.0 80 10.0 81 10.5 82 7.0 83 1.5 84 1.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048796123100180354 2 0.0 3 0.0 4 0.0 5 0.01817894782163582 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0023919668186362917 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.979917046590729E-4 24-25 2.3919668186362918E-4 26-27 7.175900455908876E-4 28-29 0.001793975113977219 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014710595934613196 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.7839336372725835E-4 84-85 0.0011959834093181459 86-87 0.0 88-89 0.0 90-91 0.011720637411317831 92-93 0.0014351800911817751 94-95 0.0 96-97 0.0 98-99 3.587950227954438E-4 100-101 0.015428185980204083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 418066.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.89773576625072 #Duplication Level Percentage of deduplicated Percentage of total 1 87.03155144896678 61.70339937955702 2 8.083825758499852 11.462498852130677 3 2.115223453982157 4.498936605810096 4 0.8717715046815449 2.4722650314983587 5 0.4773123648641654 1.6920182961051928 6 0.3091716729062228 1.315174294326905 7 0.19894073035822105 0.9873113133857461 8 0.14262545878081595 0.8089457672146058 9 0.1072086440026328 0.684076510292103 >10 0.6055812276501572 7.668286317333815 >50 0.03414024565061349 1.646396258030806 >100 0.02028133402103589 2.886345925663958 >500 0.0013520889347357259 0.6156611327657513 >1k 0.0010140667010517944 1.5586843158849535 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2631 0.6293264699832084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2299 0.5499131716044835 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1574 0.3764955772533523 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 778 0.1860950184899035 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 628 0.1502155162103591 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 620 0.1483019427554501 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 543 0.12988379825195065 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 494 0.11816316084063282 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 490 0.1172063741131783 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 420 0.10046260638272425 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.1959834093181459E-4 0.0 20-21 0.0 0.0 0.0 3.587950227954438E-4 0.0 22-23 0.0 0.0 0.0 7.175900455908876E-4 0.0 24-25 0.0 0.0 0.0 7.175900455908876E-4 0.0 26-27 0.0 0.0 0.0 0.0010763850683863313 0.0 28-29 0.0 0.0 0.0 0.004066343591681696 0.0 30-31 0.0 0.0 0.0 0.010046260638272426 0.0 32-33 0.0 0.0 0.0 0.020690512981203925 0.0 34-35 0.0 0.0 0.0 0.0319327570287945 0.0 36-37 0.0 0.0 0.0 0.04771973803179402 0.0 38-39 0.0 0.0 0.0 0.07439016805958867 0.0 40-41 0.0 0.0 0.0 0.10775810517956495 0.0 42-43 0.0 0.0 0.0 0.1591853917802452 0.0 44-45 0.0 0.0 0.0 0.22723684777044773 0.0 46-47 0.0 0.0 0.0 0.3070089411719681 0.0 48-49 0.0 0.0 0.0 0.40268761391741975 0.0 50-51 0.0 0.0 0.0 0.5255151100543933 0.0 52-53 0.0 0.0 0.0 0.6477446144867078 0.0 54-55 0.0 0.0 0.0 0.7782264044433176 0.0 56-57 0.0 0.0 0.0 0.9645606196150847 0.0 58-59 0.0 0.0 0.0 1.18043562499701 0.0 60-61 0.0 0.0 0.0 1.4180775284285256 0.0 62-63 0.0 0.0 0.0 1.6898049590256083 0.0 64-65 0.0 0.0 0.0 1.9618911846454865 0.0 66-67 0.0 0.0 0.0 2.297842924322954 0.0 68-69 0.0 0.0 0.0 2.6952682112393735 0.0 70-71 0.0 0.0 0.0 3.1651700927604733 0.0 72-73 0.0 0.0 0.0 3.6978611032707756 0.0 74-75 0.0 0.0 0.0 4.230312917099214 0.0 76-77 0.0 0.0 0.0 4.803667363526333 0.0 78-79 0.0 0.0 0.0 5.500447297795085 0.0 80-81 0.0 0.0 0.0 6.319456736496152 0.0 82-83 0.0 0.0 0.0 7.1303334880138545 0.0 84-85 0.0 0.0 0.0 8.000531016633737 0.0 86-87 0.0 0.0 0.0 8.972626331727525 0.0 88-89 0.0 0.0 0.0 10.038247549429993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1535 0.0 42.413303 1 GTATCAA 2470 0.0 33.86146 1 TAAGGTG 315 0.0 31.669617 5 AAGGTAA 285 0.0 30.013565 1 GTAAGGT 305 0.0 29.59292 4 AGGTAAG 290 0.0 29.485504 2 GGTAAGG 290 0.0 29.485504 3 TATCAAC 3135 0.0 26.517622 2 TCAACGC 3135 0.0 26.517622 4 ATCAACG 3125 0.0 26.450462 3 CAACGCA 3195 0.0 26.019638 5 GGTGTGC 405 0.0 25.801786 8 CCTATAC 95 0.001224584 25.002329 3 AACGCAG 3330 0.0 24.961803 6 TATAGGG 165 2.4217788E-6 23.032448 2 ACGCAGA 3760 0.0 21.980803 7 AAGGTGT 435 0.0 21.838503 6 CGCAGAG 3815 0.0 21.539406 8 CCCTAAG 155 3.8852028E-5 21.461313 1 GTGTGCA 490 0.0 21.325968 9 >>END_MODULE