FastQCFastQC Report
Thu 26 May 2016
SRR937164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599767
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37070.6180733518182894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28320.47218336453989634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18250.3042848306092199No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8400.14005438778725737No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.11471121285432509No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.045.871921
GTATCAA28000.045.643731
TATCAAC36450.034.795892
ATCAACG36450.034.5381363
TCAACGC36650.034.352534
CAACGCA37600.033.484585
AACGCAG38950.032.3213126
TAACGCG300.003951713431.66552476-77
CGCAGAG42750.029.2309428
ACGCAGA43100.028.988727
ACGCGAA350.00838976127.14414678-79
GCGTATT350.00839489427.14074194-95
GCAGAGT46000.026.8558399
TACATGG24300.025.8036962
AGCCGCG1151.2796088E-424.7858587
GTACATG25550.024.7313391
ACATGGG24000.024.1493
AGTACTT29900.023.99244312-13
GAGTACT28850.023.13658112-13
GTAAGGT3300.023.035254
Produced by FastQC (version 0.11.5)