##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 599767 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00962873916038 33.0 31.0 34.0 31.0 34.0 2 32.11999159673673 33.0 31.0 34.0 31.0 34.0 3 32.15716936743769 34.0 31.0 34.0 30.0 34.0 4 35.75633704421884 37.0 35.0 37.0 35.0 37.0 5 35.77499428944907 37.0 35.0 37.0 35.0 37.0 6 35.80838558973735 37.0 36.0 37.0 35.0 37.0 7 35.69301245316932 37.0 35.0 37.0 35.0 37.0 8 35.7102908296055 37.0 35.0 37.0 35.0 37.0 9 37.39369288406998 39.0 37.0 39.0 35.0 39.0 10-11 37.30393886292511 39.0 37.0 39.0 35.0 39.0 12-13 37.178834447377064 39.0 37.0 39.0 34.0 39.0 14-15 38.40841193330077 40.0 38.0 41.0 34.0 41.0 16-17 38.35046776498207 40.0 38.0 41.0 34.0 41.0 18-19 38.34880045084174 40.0 38.0 41.0 34.0 41.0 20-21 38.240997754127854 40.0 38.0 41.0 34.0 41.0 22-23 38.106661420184835 40.0 38.0 41.0 34.0 41.0 24-25 38.047790225204125 40.0 38.0 41.0 33.5 41.0 26-27 38.04101009225249 40.0 38.0 41.0 34.0 41.0 28-29 37.95226146153423 40.0 38.0 41.0 33.5 41.0 30-31 37.71789128111416 40.0 37.5 41.0 33.0 41.0 32-33 37.45815124873492 40.0 37.0 41.0 33.0 41.0 34-35 37.49930056171813 40.0 37.0 41.0 33.0 41.0 36-37 37.385312129543635 40.0 37.0 41.0 33.0 41.0 38-39 37.253701854220054 40.0 37.0 41.0 32.5 41.0 40-41 37.03990799760574 40.0 36.5 41.0 31.5 41.0 42-43 36.767447191992886 40.0 36.0 41.0 31.0 41.0 44-45 36.51693324240914 40.0 36.0 41.0 30.5 41.0 46-47 36.148749431029046 39.0 36.0 41.0 29.5 41.0 48-49 35.78696810594781 39.0 35.0 41.0 28.5 41.0 50-51 34.78883549778497 38.0 34.0 40.0 26.0 40.5 52-53 34.880617806581554 38.5 35.0 39.5 26.5 40.5 54-55 35.2451985521044 39.0 35.0 40.0 26.5 41.0 56-57 35.04010640798843 39.0 35.0 40.0 26.0 41.0 58-59 34.77957356773547 38.0 35.0 40.0 26.0 41.0 60-61 34.48843217449443 38.0 34.0 40.0 24.5 41.0 62-63 34.177839060835296 38.0 34.0 40.0 23.5 41.0 64-65 33.76942129193503 37.0 34.0 40.0 22.0 41.0 66-67 33.42975522161106 37.0 33.5 39.5 21.5 41.0 68-69 32.96051966847126 36.0 33.0 39.0 19.5 41.0 70-71 32.48156117292215 35.5 32.0 39.0 18.0 40.0 72-73 32.037678798600126 35.0 32.0 38.0 14.5 40.0 74-75 31.57641550802228 35.0 31.5 37.5 9.5 39.5 76-77 31.101028399361752 35.0 31.0 37.0 7.0 39.0 78-79 30.60317506631742 35.0 31.0 36.0 4.0 39.0 80-81 30.13154775104332 34.0 30.0 36.0 2.0 38.0 82-83 29.65404565439579 34.0 30.0 35.5 2.0 37.0 84-85 29.139652565079437 34.0 29.0 35.0 2.0 37.0 86-87 28.716697484189694 34.0 29.0 35.0 2.0 36.0 88-89 28.27678415117871 34.0 28.5 35.0 2.0 36.0 90-91 27.785240768498433 34.0 27.0 35.0 2.0 35.5 92-93 27.30423397752794 33.0 26.0 35.0 2.0 35.0 94-95 26.780978279898694 33.0 25.5 35.0 2.0 35.0 96-97 26.098354861137743 33.0 24.5 35.0 2.0 35.0 98-99 25.229715872997346 32.0 20.0 34.5 2.0 35.0 100-101 22.715166889808877 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.020177987330033176 1101 2 0.01878729294787007 1101 3 0.026489127569348625 1101 4 -0.039958641530432715 1101 5 0.04619537682455643 1101 6 0.0012332888294110944 1101 7 -0.03212182812585951 1101 8 -0.031931057092883464 1101 9 0.14193636415224375 1101 10-11 0.072772155811208 1101 12-13 0.13324393560506564 1101 14-15 0.10697927340793001 1101 16-17 -0.09194793555120384 1101 18-19 0.038241025332183654 1101 20-21 0.08943949461160372 1101 22-23 -0.006762874407201025 1101 24-25 0.07100380181498167 1101 26-27 -0.02353410951130286 1101 28-29 -0.07311563035643331 1101 30-31 0.01232034360343448 1101 32-33 -0.008911815942511225 1101 34-35 0.08345138854714662 1101 36-37 0.22401265807876314 1101 38-39 0.10687631320981694 1101 40-41 0.01624989390622744 1101 42-43 -0.0439457746919345 1101 44-45 0.22054292096080275 1101 46-47 0.4245132960134512 1101 48-49 0.42293128753542675 1101 50-51 0.39421605602148446 1101 52-53 0.36072980308422586 1101 54-55 0.27852557987129245 1101 56-57 0.2989794706704956 1101 58-59 0.25188479241369066 1101 60-61 0.28864722215902106 1101 62-63 0.35632874794247726 1101 64-65 0.4416848968392557 1101 66-67 0.39499114605347785 1101 68-69 0.5159445770947357 1101 70-71 0.40866244203363067 1101 72-73 0.617361471648092 1101 74-75 0.4147486044834423 1101 76-77 0.3541716895351428 1101 78-79 0.30449247085557474 1101 80-81 0.1534905895601888 1101 82-83 0.12046990160804327 1101 84-85 0.1986283177918189 1101 86-87 0.35681124913960716 1101 88-89 0.5156209695826384 1101 90-91 0.3770143454150876 1101 92-93 0.26721432126975486 1101 94-95 0.22368246974093609 1101 96-97 0.47765231608270753 1101 98-99 0.5367685414694918 1101 100-101 1.0582444744625015 1102 1 0.03571497647986277 1102 2 -0.06590079007911953 1102 3 -0.04764164415116312 1102 4 0.037823244207459084 1102 5 0.023447949708689464 1102 6 0.007465181277460431 1102 7 0.010769880490236972 1102 8 -0.10488492016887108 1102 9 -0.12226548258780667 1102 10-11 0.005979465407257578 1102 12-13 0.03609996198255061 1102 14-15 0.1870916224623329 1102 16-17 0.20788220541468405 1102 18-19 0.0651915096475264 1102 20-21 0.25859396239101784 1102 22-23 -0.03323476674999171 1102 24-25 -0.03748230816315612 1102 26-27 -0.08843710171436214 1102 28-29 0.14215997283391602 1102 30-31 0.21235731524447488 1102 32-33 0.022237120563630697 1102 34-35 0.27663347585284725 1102 36-37 0.311793991215616 1102 38-39 0.30180830381033985 1102 40-41 0.43160950561189537 1102 42-43 0.2715884326281852 1102 44-45 0.46560240451565704 1102 46-47 0.7874662644020418 1102 48-49 0.5677972949402843 1102 50-51 0.43262774905949897 1102 52-53 0.44927655444796954 1102 54-55 0.5867434152014468 1102 56-57 0.8608793393619649 1102 58-59 0.6663931353736814 1102 60-61 0.8259734488675434 1102 62-63 0.7143740928049951 1102 64-65 0.928598777914253 1102 66-67 1.0083894845663437 1102 68-69 0.9879138893169497 1102 70-71 0.9462810867927374 1102 72-73 0.9597339984295097 1102 74-75 0.7630388963357362 1102 76-77 0.9198026020373469 1102 78-79 0.8310594237344944 1102 80-81 0.8670472087996508 1102 82-83 0.7313499582364926 1102 84-85 0.6647594658073253 1102 86-87 0.7375642206859148 1102 88-89 0.5522324331596522 1102 90-91 0.6305769774801568 1102 92-93 0.6110104535887615 1102 94-95 0.5276337645378 1102 96-97 0.7344585188978172 1102 98-99 0.4750976074947886 1102 100-101 0.7087897732749369 1103 1 -0.09253260289033349 1103 2 -0.07631441752943857 1103 3 -0.09162179057636877 1103 4 0.040142095604196015 1103 5 -0.12417484594062245 1103 6 -0.09831820712930295 1103 7 -0.025312261470283204 1103 8 -0.04082219752859828 1103 9 -0.0835472798587844 1103 10-11 -0.1595402151442471 1103 12-13 -0.21003754743194492 1103 14-15 -0.33842846520281 1103 16-17 -0.36485712651184343 1103 18-19 -0.4318145088490084 1103 20-21 -0.30317704976133086 1103 22-23 -0.2739381573280255 1103 24-25 -0.35882049067220123 1103 26-27 -0.35404142797522553 1103 28-29 -0.28636274749937485 1103 30-31 -0.5122998611510781 1103 32-33 -0.4281849205684125 1103 34-35 -0.2842334047746533 1103 36-37 -0.464293511074402 1103 38-39 -0.6137563288713821 1103 40-41 -0.48204254115189116 1103 42-43 -0.3864064309548141 1103 44-45 -0.45216064177995463 1103 46-47 -0.2667086858961625 1103 48-49 -0.26330058874734874 1103 50-51 -0.5068107055371556 1103 52-53 -0.6192618736863054 1103 54-55 -0.3985733531305442 1103 56-57 -0.45464220712702996 1103 58-59 -0.25621541129850556 1103 60-61 -0.2310292448676563 1103 62-63 -0.31685152278473794 1103 64-65 -0.10662164019145592 1103 66-67 -0.10908654661855621 1103 68-69 -0.029744664479721905 1103 70-71 -0.012177207808257151 1103 72-73 0.027594751996840472 1103 74-75 0.0013776352334105013 1103 76-77 -0.002666847477168943 1103 78-79 -0.0897722415638178 1103 80-81 0.04394317091094635 1103 82-83 -0.08807987912161153 1103 84-85 0.0443496720103802 1103 86-87 0.30344792867687786 1103 88-89 0.20074154911240427 1103 90-91 0.10807631592050626 1103 92-93 0.006521347271032596 1103 94-95 -0.18521457206374947 1103 96-97 -0.056476756819890284 1103 98-99 -0.0734540857586623 1103 100-101 0.4232088899740596 1104 1 -0.008558002218503447 1104 2 0.1702193111330672 1104 3 0.01515993287602413 1104 4 0.0983446046876324 1104 5 -0.07411957412325165 1104 6 -0.09015383131522015 1104 7 -0.12715379845526797 1104 8 -0.11756218861655299 1104 9 0.1711101205662473 1104 10-11 0.09454197276279785 1104 12-13 0.0575744529793738 1104 14-15 0.09321657832910546 1104 16-17 -0.06677171655778125 1104 18-19 0.07118259473637778 1104 20-21 -0.05951831252954776 1104 22-23 -0.18354517042187268 1104 24-25 -0.14020974137487485 1104 26-27 -0.13368468909814624 1104 28-29 -0.28722503069673877 1104 30-31 -0.29682975077882645 1104 32-33 -0.29362803772752955 1104 34-35 -0.13625489651387568 1104 36-37 -0.29296908692960955 1104 38-39 -0.26639223755720565 1104 40-41 -0.10601958406681433 1104 42-43 -0.09529105424953599 1104 44-45 -0.15707876236677265 1104 46-47 -0.05682252870140303 1104 48-49 0.01319346417648859 1104 50-51 -0.0834018054891672 1104 52-53 -0.05179919287461843 1104 54-55 0.03971394891922131 1104 56-57 -0.00881180162897266 1104 58-59 0.27795456882078184 1104 60-61 0.2632325550226611 1104 62-63 0.29673956002871904 1104 64-65 0.4682984513283941 1104 66-67 0.27788901381196496 1104 68-69 0.16590685742333733 1104 70-71 0.1571698563584647 1104 72-73 0.3555740743386515 1104 74-75 0.2076443453177319 1104 76-77 0.4119671159834475 1104 78-79 0.3997196861800596 1104 80-81 0.2628302792951196 1104 82-83 0.5107334906596996 1104 84-85 0.6788637084812095 1104 86-87 0.7296386293007195 1104 88-89 0.37739499053284575 1104 90-91 0.5045766244143728 1104 92-93 0.7127559225778164 1104 94-95 0.8585851297255154 1104 96-97 0.9538502895227445 1104 98-99 0.899973666692155 1104 100-101 1.3405550286591925 1105 1 -0.0461512603387817 1105 2 0.06441114991024932 1105 3 -0.01687764332268671 1105 4 0.13751301520532877 1105 5 0.14874174422812558 1105 6 -0.051687699935001774 1105 7 0.16320308444892362 1105 8 0.17483898052183378 1105 9 0.044148288157913385 1105 10-11 0.04715234716002925 1105 12-13 0.04076240221056793 1105 14-15 -0.05499843850284947 1105 16-17 0.013340704968925365 1105 18-19 0.016561199958836426 1105 20-21 0.15522556882793026 1105 22-23 0.12122122935976165 1105 24-25 0.05994265604118709 1105 26-27 0.10294357493606299 1105 28-29 -0.21245789167389972 1105 30-31 -0.05600202809663557 1105 32-33 0.03635501658963847 1105 34-35 -0.18054681717623566 1105 36-37 0.0971754062921164 1105 38-39 0.23533644955043087 1105 40-41 0.2053843486963558 1105 42-43 0.07928359773845983 1105 44-45 0.21525570446972608 1105 46-47 0.1700520163809358 1105 48-49 0.19032987123680556 1105 50-51 -1.1133575782196203E-5 1105 52-53 0.12211440688881936 1105 54-55 0.2616355779793693 1105 56-57 0.1013450880989808 1105 58-59 0.2279875503511235 1105 60-61 0.4530142514106146 1105 62-63 0.690730950712009 1105 64-65 0.7989397146347343 1105 66-67 0.5890242608322538 1105 68-69 0.42874975160949447 1105 70-71 0.5618233796957384 1105 72-73 0.76574631753763 1105 74-75 0.7387777726673761 1105 76-77 0.5428056998247719 1105 78-79 0.7131949159134798 1105 80-81 0.9724080019773176 1105 82-83 0.7900458257520491 1105 84-85 0.774689270304517 1105 86-87 0.5884122850190359 1105 88-89 0.5675761614909014 1105 90-91 0.6473525319751019 1105 92-93 0.8771432622193416 1105 94-95 0.9854815677202389 1105 96-97 0.8329033215489474 1105 98-99 1.1018817619519083 1105 100-101 1.1610430984435602 1106 1 -0.21994110783550624 1106 2 -0.2281080582011299 1106 3 -0.10902679451499608 1106 4 -0.1128894009510617 1106 5 -0.09573229370685965 1106 6 -0.15626611715343586 1106 7 -0.665255620185917 1106 8 -0.7343684670810973 1106 9 -0.52042913339168 1106 10-11 -0.8913443588355534 1106 12-13 -0.4261668721127947 1106 14-15 -0.3597830421436754 1106 16-17 -0.3513188592600116 1106 18-19 -0.6322643256713434 1106 20-21 -0.468540216671812 1106 22-23 -0.28703410991439426 1106 24-25 -0.5702140788287977 1106 26-27 -0.8296766105902265 1106 28-29 -0.1979107550515451 1106 30-31 -0.3649684408677487 1106 32-33 -0.35961833267440113 1106 34-35 -0.30689304442105225 1106 36-37 -0.6521068824186571 1106 38-39 -0.5677368482707124 1106 40-41 -0.7160926430561858 1106 42-43 -0.6077341251669068 1106 44-45 -0.7795813377300362 1106 46-47 -0.7581312198746843 1106 48-49 -0.3774959199050585 1106 50-51 -0.5078250596639649 1106 52-53 -0.10023296537516302 1106 54-55 -0.26703553600812313 1106 56-57 -0.426877974910262 1106 58-59 -0.4048284500471411 1106 60-61 -0.43041781908274146 1106 62-63 -0.43964151240675875 1106 64-65 -0.3231479540066786 1106 66-67 -0.020432933704846334 1106 68-69 -0.31359189555865896 1106 70-71 0.03344059480998851 1106 72-73 -0.13008183002049023 1106 74-75 0.11155618656691502 1106 76-77 -0.07925292847545862 1106 78-79 -0.22630368185290095 1106 80-81 -0.6518099831208382 1106 82-83 -0.4976194967616827 1106 84-85 -0.5756160681399116 1106 86-87 -0.29071351773896836 1106 88-89 0.25310660406570307 1106 90-91 0.16752934616670245 1106 92-93 0.11798933181659166 1106 94-95 -0.0818629834820328 1106 96-97 -0.11590477987651937 1106 98-99 0.15905182218987335 1106 100-101 0.800687727639886 1107 1 0.15024513204910406 1107 2 0.08800657053878069 1107 3 -0.018966658971393713 1107 4 0.11392871819580108 1107 5 0.02970122310882317 1107 6 0.09844556102065383 1107 7 0.22246750177151142 1107 8 0.22039464978549006 1107 9 0.11864093989520086 1107 10-11 0.16523289995939905 1107 12-13 0.12166448321720935 1107 14-15 0.10235516354745755 1107 16-17 1.9036258393612115E-4 1107 18-19 -0.054945872438132426 1107 20-21 0.04022292140660966 1107 22-23 -0.025010978411650342 1107 24-25 0.05374123844718781 1107 26-27 0.1888479474082274 1107 28-29 -0.020504272715690774 1107 30-31 0.2151817919321104 1107 32-33 0.21125835234050072 1107 34-35 0.23702605365186002 1107 36-37 0.3084285042200108 1107 38-39 0.13918722397678351 1107 40-41 0.04795621839873121 1107 42-43 -0.024566509270755432 1107 44-45 -0.09235908015475758 1107 46-47 0.12009695580169932 1107 48-49 0.18016844527784315 1107 50-51 0.28082687538540796 1107 52-53 0.06723047072949839 1107 54-55 0.14446604957631592 1107 56-57 0.16807679085808758 1107 58-59 0.22009200558300535 1107 60-61 0.16890923554833392 1107 62-63 0.37799373953677673 1107 64-65 0.13573953843661002 1107 66-67 0.231431056028633 1107 68-69 0.16869622475485357 1107 70-71 0.6625132665462843 1107 72-73 0.44963753744267976 1107 74-75 0.5038769731016473 1107 76-77 0.43663075644399285 1107 78-79 0.957707634659446 1107 80-81 0.8258009282455205 1107 82-83 0.8156713068485679 1107 84-85 0.6944423704571356 1107 86-87 0.6936639680225909 1107 88-89 0.8868206897827129 1107 90-91 0.75517265860638 1107 92-93 0.6251443478845573 1107 94-95 0.8029885867238278 1107 96-97 1.0907210009840433 1107 98-99 1.2225162976743 1107 100-101 1.586205614517187 1108 1 -0.02151343487953028 1108 2 0.14044540091836666 1108 3 0.11550150737179621 1108 4 -0.008486885227789287 1108 5 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-0.5878479573478081 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2739.0 3 534.0 4 424.0 5 458.0 6 424.0 7 533.0 8 602.0 9 765.0 10 988.0 11 1377.0 12 1746.0 13 2428.0 14 2833.0 15 3950.0 16 5167.0 17 6729.0 18 5946.0 19 3893.0 20 3488.0 21 3655.0 22 3755.0 23 4047.0 24 4659.0 25 5251.0 26 5932.0 27 7127.0 28 8262.0 29 10105.0 30 12446.0 31 15845.0 32 20982.0 33 28047.0 34 39825.0 35 58308.0 36 86907.0 37 122990.0 38 102090.0 39 14506.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0981630981631 19.17531631817346 15.416771131056844 22.309749452606596 2 14.81667755534428 20.084179781814292 41.28067557700245 23.818467085838982 3 18.832245086179817 24.89507381590467 30.540536928001927 25.732144169913585 4 12.154139465404338 16.756200383978506 38.21304624618402 32.876613904433135 5 12.902045378974087 37.516604814602545 34.95491009589437 14.626439710528999 6 30.676720881883025 36.89693092406527 17.88483778887485 14.541510405176853 7 27.125757109812675 33.58592611410464 21.185704162265516 18.102612613817172 8 26.056869947093837 34.666570858496904 20.268775337640243 19.007783856769013 9 25.835456892446256 16.662684123018824 20.940771499469456 36.561087485065464 10-11 24.055551377503697 27.090154002991483 27.834432160971982 21.019862458532835 12-13 25.38045912466826 24.506648216112147 28.34416300663819 21.768729652581406 14-15 22.168081591935053 25.72089335381988 27.126628083112553 24.984396971132515 16-17 20.988777752709467 29.092527136446815 27.952838150627123 21.96585696021659 18-19 20.930963147147388 27.071824127639804 31.043523596587093 20.95368912862571 20-21 21.549497224626467 26.088093463753054 31.43916735452264 20.923241957097837 22-23 21.149203081740303 26.039820014603343 31.47062705829475 21.3403498453616 24-25 21.046199034617114 26.305263492052884 31.50810584125149 21.140431632078514 26-27 20.679566227212725 26.62071615954016 31.24124851705182 21.458469096195298 28-29 20.677185512467876 26.93819370406639 31.276382060700048 21.108238722765694 30-31 20.769552139037433 26.60586564171123 31.090992647058822 21.53358957219251 32-33 20.47980361339981 26.661335886760195 31.131420435603257 21.727440064236735 34-35 21.2098650301827 26.658544411688023 30.89030610539545 21.241284452733826 36-37 21.204584549243634 26.27352071092427 30.983970452230913 21.537924287601182 38-39 20.695892200871036 26.758715987992986 30.98715336345948 21.5582384476765 40-41 21.148404742789783 26.61115088591056 31.046175618224304 21.194268753075356 42-43 20.827147508222936 26.674558902283042 30.767103580162026 21.731190009331996 44-45 20.937393203636066 26.962024153003 30.76905080018416 21.331531843176773 46-47 21.37780221469803 27.290069957183395 29.89115357490232 21.440974253216257 48-49 21.069674316150824 27.401515189498966 29.719591336712675 21.809219157637536 50-51 21.30228889113582 27.59011709770175 29.648623331130043 21.458970680032387 52-53 21.493821918815733 27.441395149805096 29.5950314587359 21.46975147264327 54-55 21.124307774072165 27.66221296700964 29.641099606161365 21.57237965275683 56-57 21.163280310118967 27.851724368399942 29.39588624515439 21.589109076326693 58-59 21.128259445877497 27.437567661485414 29.509596236350756 21.924576656286337 60-61 20.916251795917002 27.526061679307695 29.797103144107723 21.76058338066758 62-63 20.938619111702543 27.510924326225318 29.980728410933434 21.569728151138712 64-65 20.914274354142442 27.531056626972987 29.75413642863825 21.800532590246316 66-67 20.72731889699323 27.603736831378157 29.658676672536092 22.010267599092522 68-69 21.14312636423098 27.354204274820127 29.704628590856423 21.798040770092463 70-71 21.023578766579735 27.48547733093642 29.370625865637955 22.120318036845894 72-73 20.89024588897196 27.401312354594705 29.455719518664086 22.252722237769248 74-75 20.958880192604735 27.793274038208516 29.170394540638213 22.07745122854854 76-77 21.102289446223537 27.47995491788555 29.259929803571982 22.15782583231893 78-79 21.079404383581785 27.465973607616295 29.37948825909849 22.07513374970343 80-81 21.143132109802036 27.452931303972257 29.148548863141766 22.255387723083942 82-83 21.154880726035774 27.487561357841944 29.196475411753305 22.161082504368977 84-85 21.03268351689478 27.63479736954662 28.98327229469632 22.349246818862277 86-87 21.106142236428024 27.593990678417086 28.899580370039608 22.400286715115282 88-89 21.124916881355027 27.378112145847744 28.860130116182326 22.636840856614906 90-91 20.85776966853469 27.52943220817585 29.081578094350153 22.531220028939305 92-93 20.994687902803406 27.354669157173422 29.153631464590056 22.49701147543312 94-95 20.812627129616867 27.200036755338925 29.15475250709002 22.83258360795419 96-97 20.99901086378993 26.998656646093078 29.008173740438632 22.994158749678366 98-99 20.920506286812316 27.48560925686119 28.636367433894485 22.957517022432015 100-101 21.336961914928807 27.040200073173054 28.771377641455377 22.851460370442762 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 857.0 1 519.5 2 217.5 3 248.0 4 185.5 5 158.5 6 282.5 7 585.0 8 1025.5 9 1228.5 10 1070.5 11 900.0 12 848.5 13 908.5 14 986.0 15 1001.0 16 1041.0 17 1120.0 18 1209.0 19 1314.0 20 1485.0 21 1774.5 22 2152.0 23 2549.5 24 2976.5 25 3656.0 26 4757.0 27 6096.5 28 7061.0 29 7955.0 30 9022.0 31 10337.0 32 12188.5 33 13331.5 34 13914.5 35 14899.0 36 16295.0 37 17479.0 38 18109.0 39 18435.0 40 18738.0 41 19474.0 42 19688.0 43 19769.5 44 20267.0 45 20864.0 46 21085.5 47 21224.0 48 21813.0 49 21414.5 50 20308.0 51 19409.5 52 18456.5 53 17967.0 54 17766.0 55 16350.0 56 14555.5 57 13005.0 58 11766.0 59 10352.0 60 8284.5 61 6873.0 62 5763.5 63 4098.5 64 2690.5 65 2051.5 66 1542.5 67 1131.5 68 890.0 69 696.5 70 526.0 71 354.0 72 245.5 73 183.0 74 120.5 75 85.0 76 66.0 77 43.5 78 22.0 79 13.5 80 10.5 81 9.5 82 11.5 83 9.0 84 5.5 85 4.0 86 2.5 87 2.5 88 1.5 89 0.5 90 1.0 91 1.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2462622985259276 2 0.21441659844572977 3 0.20858099895459406 4 0.21608391258605425 5 0.21608391258605425 6 0.23525802519978592 7 0.23992650479269448 8 0.22492067752977407 9 0.22041892935089794 10-11 0.2337574424734939 12-13 0.23525802519978592 14-15 0.22100248930001148 16-17 0.2337574424734939 18-19 0.22225297490525486 20-21 0.21491679268782712 22-23 0.21241582147734037 24-25 0.20808080471249665 26-27 0.2162506440000867 28-29 0.22492067752977407 30-31 0.22792184298235815 32-33 0.22600443172098497 34-35 0.23525802519978592 36-37 0.2131661128404864 38-39 0.21399976991064865 40-41 0.20983148455983738 42-43 0.2148334269808109 44-45 0.22900559717356905 46-47 0.23359071105946141 48-49 0.23775899641027268 50-51 0.2368419736330942 52-53 0.25393194357142024 54-55 0.23767563070325642 56-57 0.2145833298597622 58-59 0.19991096542490666 60-61 0.19791018845651728 62-63 0.20299549658450697 64-65 0.19724326280038748 66-67 0.19757672562845238 68-69 0.20266203375644207 70-71 0.2034123251195881 72-73 0.20416261648273415 74-75 0.20599666203709108 76-77 0.21733439819129763 78-79 0.2102483130949185 80-81 0.2089978274896752 82-83 0.20491290784588012 84-85 0.20658022198620463 86-87 0.21066514162999964 88-89 0.20424598218975035 90-91 0.21224909006330792 92-93 0.20466281072483147 94-95 0.20249530234240964 96-97 0.21091523875104834 98-99 0.21541698692992445 100-101 0.1977434570424848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 599767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.36159598929167 #Duplication Level Percentage of deduplicated Percentage of total 1 85.95743549002928 59.62144912735011 2 8.768079463680678 12.163359707236486 3 2.2563222952012687 4.695063463841451 4 0.9588688765850407 2.6603470249759025 5 0.5253928940377491 1.822104482594554 6 0.3329025816974491 1.3854392625294376 7 0.21569678682007554 1.0472751368521716 8 0.14415062697610048 0.7998814039935498 9 0.1306728854406079 0.8157311898017793 >10 0.6445787135959968 7.975212568384368 >50 0.04208905329143216 1.9736560696086265 >100 0.02212677377050069 3.212461830167854 >500 9.620336421916183E-4 0.4327323044834447 >1k 7.215252316437136E-4 1.3952864281802357 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3707 0.6180733518182894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2832 0.47218336453989634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1825 0.3042848306092199 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 840 0.14005438778725737 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 688 0.11471121285432509 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.336570701622463E-5 0.0 24-25 0.0 0.0 0.0 5.001942420973478E-4 0.0 26-27 0.0 0.0 0.0 6.66925656129797E-4 0.0 28-29 0.0 0.0 0.0 0.0020841426754056157 0.0 30-31 0.0 0.0 0.0 0.007336182217427767 0.0 32-33 0.0 0.0 0.0 0.015172558676952882 0.0 34-35 0.0 0.0 0.0 0.025259809225916065 0.0 36-37 0.0 0.0 0.0 0.042433144871258335 0.0 38-39 0.0 0.0 0.0 0.07111094808483961 0.0 40-41 0.0 0.0 0.0 0.10078913978261558 0.0 42-43 0.0 0.0 0.0 0.1373866851627382 0.0 44-45 0.0 0.0 0.0 0.18573879523214848 0.0 46-47 0.0 0.0 0.0 0.2500137553416577 0.0 48-49 0.0 0.0 0.0 0.3239591374650489 0.0 50-51 0.0 0.0 0.0 0.41065947276192255 0.0 52-53 0.0 0.0 0.0 0.5001108763903316 0.0 54-55 0.0 0.0 0.0 0.6024839646062554 0.0 56-57 0.0 0.0 0.0 0.7431219123426265 0.0 58-59 0.0 0.0 0.0 0.8937637449209443 0.0 60-61 0.0 0.0 0.0 1.0722497236426811 0.0 62-63 0.0 0.0 0.0 1.2673254780606469 0.0 64-65 0.0 0.0 0.0 1.4630681581347424 0.0 66-67 0.0 0.0 0.0 1.697825989092431 0.0 68-69 0.0 0.0 0.0 1.9843539241071948 0.0 70-71 0.0 0.0 0.0 2.32165157469484 0.0 72-73 0.0 0.0 0.0 2.692378873795991 0.0 74-75 0.0 0.0 0.0 3.070942549356667 1.6673141403244926E-4 76-77 0.0 0.0 0.0 3.484769918985206 1.6673141403244926E-4 78-79 0.0 0.0 0.0 3.9839637725983588 1.6673141403244926E-4 80-81 0.0 0.0 0.0 4.584863788771306 1.6673141403244926E-4 82-83 0.0 0.0 0.0 5.160253898597288 3.334628280648985E-4 84-85 0.0 0.0 0.0 5.782162072938324 3.334628280648985E-4 86-87 0.0 0.0 0.0 6.482350646167595 3.334628280648985E-4 88-89 0.0 0.0 0.0 7.255234115915014 3.334628280648985E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1450 0.0 45.87192 1 GTATCAA 2800 0.0 45.64373 1 TATCAAC 3645 0.0 34.79589 2 ATCAACG 3645 0.0 34.538136 3 TCAACGC 3665 0.0 34.35253 4 CAACGCA 3760 0.0 33.48458 5 AACGCAG 3895 0.0 32.321312 6 TAACGCG 30 0.0039517134 31.665524 76-77 CGCAGAG 4275 0.0 29.230942 8 ACGCAGA 4310 0.0 28.98872 7 ACGCGAA 35 0.008389761 27.144146 78-79 GCGTATT 35 0.008394894 27.140741 94-95 GCAGAGT 4600 0.0 26.855839 9 TACATGG 2430 0.0 25.803696 2 AGCCGCG 115 1.2796088E-4 24.785858 7 GTACATG 2555 0.0 24.731339 1 ACATGGG 2400 0.0 24.149 3 AGTACTT 2990 0.0 23.992443 12-13 GAGTACT 2885 0.0 23.136581 12-13 GTAAGGT 330 0.0 23.03525 4 >>END_MODULE