FastQCFastQC Report
Thu 26 May 2016
SRR937164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599767
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36560.6095700497026345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31750.5293722395530265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.3669758422854208No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC10510.17523471614810418No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.13305166839789453No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA7920.1320512799136998No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA6770.11287716729996816No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA6530.10887561336318938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.10053904266156691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.044.377371
GGTAAGG3950.037.2744263
AAGGTAA3900.035.3431851
GTATCAA33100.035.0375821
TCGTACT550.002580022434.5416954
GTAAGGT4100.033.5939064
AGGTAAG4100.032.4354972
TAAGGTG4400.031.3060235
ATACGAC650.005844980529.2275873
ACCGCCC856.398289E-427.9451248
ATCAACG41900.027.6580873
TCAACGC42550.027.1239574
TATCAAC42750.027.1081622
CAACGCA43200.026.718075
GCCGCGC1457.867293E-726.2106028
AACGCAG44650.025.9567876
GTATAGA2059.476935E-1025.5041681
GGTGTGC7000.025.110698
GTGTGCA7600.023.7533559
CATACGA1201.7107492E-423.7474162
Produced by FastQC (version 0.11.5)