##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937164_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 599767 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.835302709218748 31.0 31.0 34.0 30.0 34.0 2 32.08171173138902 31.0 31.0 34.0 30.0 34.0 3 32.11643521567542 33.0 31.0 34.0 30.0 34.0 4 35.865182645927504 37.0 35.0 37.0 35.0 37.0 5 35.75692560611037 37.0 35.0 37.0 35.0 37.0 6 35.812390478302405 37.0 35.0 37.0 35.0 37.0 7 35.74721350124298 37.0 35.0 37.0 33.0 37.0 8 35.71226993149006 37.0 35.0 37.0 33.0 37.0 9 37.358635936955515 39.0 37.0 39.0 34.0 39.0 10-11 37.24926263032144 39.0 37.0 39.0 34.0 39.0 12-13 37.1316294494362 39.0 37.0 39.0 33.0 39.0 14-15 38.19740916055735 40.0 37.5 41.0 33.0 41.0 16-17 38.15021916844374 40.0 37.0 41.0 33.0 41.0 18-19 38.16456223833589 40.0 37.5 41.0 33.0 41.0 20-21 38.048530512682426 40.0 37.0 41.0 33.0 41.0 22-23 37.88273946382512 40.0 37.0 41.0 33.0 41.0 24-25 37.795957430135374 39.5 37.0 41.0 32.5 41.0 26-27 37.70139904329514 39.0 37.0 41.0 32.5 41.0 28-29 37.5400989050748 39.0 37.0 41.0 32.0 41.0 30-31 37.59850658672451 39.0 37.0 41.0 33.0 41.0 32-33 37.509745451150195 39.0 36.0 41.0 33.0 41.0 34-35 37.343395852055885 39.0 36.0 41.0 32.0 41.0 36-37 37.1129571983787 39.0 36.0 41.0 31.0 41.0 38-39 36.934882212592555 39.0 35.5 41.0 31.0 41.0 40-41 36.71175723239192 39.0 35.0 40.0 31.0 41.0 42-43 36.47406993048968 39.0 35.0 40.0 30.0 41.0 44-45 36.1192354697741 39.0 35.0 40.0 30.0 41.0 46-47 35.708546819014714 38.0 35.0 40.0 28.5 41.0 48-49 35.29683193640197 38.0 35.0 40.0 27.0 41.0 50-51 34.99692630638231 38.0 34.0 40.0 26.0 41.0 52-53 34.70271788878014 38.0 34.0 40.0 25.5 41.0 54-55 34.43259715856324 38.0 34.0 40.0 24.0 41.0 56-57 34.114764566906814 38.0 33.0 40.0 23.5 41.0 58-59 33.77226239523014 37.0 33.0 40.0 23.0 41.0 60-61 33.39089429728544 37.0 32.5 40.0 22.0 41.0 62-63 32.9642819628289 36.0 32.0 39.0 20.5 40.5 64-65 32.52560244228175 36.0 31.0 39.0 19.5 40.0 66-67 32.05515641907608 35.0 31.0 38.5 18.0 40.0 68-69 31.550261184760082 35.0 30.5 38.0 15.0 40.0 70-71 31.10610537091904 35.0 30.0 37.5 12.0 40.0 72-73 30.579232435262362 34.0 29.0 37.0 8.0 39.0 74-75 30.045719421041838 34.0 29.0 36.0 7.0 39.0 76-77 28.946544241347056 32.5 28.0 35.0 7.0 37.5 78-79 29.549820180169966 34.0 29.0 35.5 3.5 38.0 80-81 29.328477558785327 34.0 29.0 35.0 2.0 37.0 82-83 28.92029904946421 34.0 29.0 35.0 2.0 37.0 84-85 28.487834442375124 33.5 28.5 35.0 2.0 36.0 86-87 27.97507532091629 33.0 27.0 35.0 2.0 36.0 88-89 27.368188813322504 33.0 26.0 35.0 2.0 35.0 90-91 26.714954307255987 32.0 25.0 34.0 2.0 35.0 92-93 26.16956918269928 32.0 24.5 34.0 2.0 35.0 94-95 25.56087864120567 32.0 23.0 34.0 2.0 35.0 96-97 24.87684217371079 31.0 19.0 34.0 2.0 35.0 98-99 23.844129470277625 31.0 4.5 34.0 2.0 35.0 100-101 21.248766604364697 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08089092193309355 1101 2 0.025290898594676037 1101 3 0.03195454518728269 1101 4 0.03935513817994263 1101 5 0.044586130987653405 1101 6 -0.05212443158664115 1101 7 0.07026827463651131 1101 8 -0.03796954980916922 1101 9 -0.08375884063521966 1101 10-11 0.04692976945936067 1101 12-13 0.00695037987443925 1101 14-15 -0.05814396591173221 1101 16-17 -0.1311081771900291 1101 18-19 -0.005721405194499596 1101 20-21 -0.10192261989833895 1101 22-23 -0.1443624846680791 1101 24-25 -0.188105792533257 1101 26-27 -0.11380969541585984 1101 28-29 -0.18559632605422394 1101 30-31 -0.15482115296364185 1101 32-33 -0.08511659664268478 1101 34-35 -0.05360154698693975 1101 36-37 0.04077122774315001 1101 38-39 -0.07787843111537285 1101 40-41 4.3294448295938537E-4 1101 42-43 0.13856159616297958 1101 44-45 0.10547558223863973 1101 46-47 0.19658509286136905 1101 48-49 0.26128310580410385 1101 50-51 0.5236434082328856 1101 52-53 0.4950571804155217 1101 54-55 0.36937063093468936 1101 56-57 0.5178895608027361 1101 58-59 0.4225475829346621 1101 60-61 0.2573580777099167 1101 62-63 0.43310033077661814 1101 64-65 0.39415336445443216 1101 66-67 0.47051182950628245 1101 68-69 0.4384955262127974 1101 70-71 0.37464986345879225 1101 72-73 0.5207523001220906 1101 74-75 0.34907671248453553 1101 76-77 0.5741041533146891 1101 78-79 0.34062221573808316 1101 80-81 0.3519280915285883 1101 82-83 0.4183154696752318 1101 84-85 0.558936200157234 1101 86-87 0.3267089088647879 1101 88-89 0.3274079916412802 1101 90-91 0.27137734717442896 1101 92-93 0.14470214941531978 1101 94-95 0.14165464579004095 1101 96-97 0.3950286558655698 1101 98-99 0.5165582420084576 1101 100-101 1.0813905940197124 1102 1 -0.042368708686982615 1102 2 -0.09281704807143143 1102 3 -0.0982843100338755 1102 4 -0.08654316744001278 1102 5 0.0404968832881778 1102 6 -0.18790048080497002 1102 7 -0.26224756001472826 1102 8 -0.14759243907055009 1102 9 -0.2927854470596003 1102 10-11 -0.2759431770356997 1102 12-13 -0.3056307011710757 1102 14-15 -0.13413516113515556 1102 16-17 -0.27158001953558397 1102 18-19 -0.30293919052741813 1102 20-21 -0.07764632990383546 1102 22-23 -0.19048164650387633 1102 24-25 -0.4212379747021302 1102 26-27 -0.3802074734333871 1102 28-29 -0.3880759258658131 1102 30-31 -0.2627868969620053 1102 32-33 -0.25500358972990256 1102 34-35 -0.32611773688170587 1102 36-37 -0.35612131240594636 1102 38-39 -0.07704920062548126 1102 40-41 -0.025443914953754643 1102 42-43 -0.2150310342442836 1102 44-45 -0.6177926713578756 1102 46-47 -0.17569367594351348 1102 48-49 0.0522344611239447 1102 50-51 0.033786268759001814 1102 52-53 0.14107443998802438 1102 54-55 0.005620338187711127 1102 56-57 -0.1495462519549804 1102 58-59 -0.21345279599176337 1102 60-61 -0.10996339926109755 1102 62-63 0.05052081543404796 1102 64-65 0.039458163399757495 1102 66-67 0.09500856784442746 1102 68-69 -0.05043205936800277 1102 70-71 -0.029842585642704478 1102 72-73 -0.14999944780762675 1102 74-75 -0.15394417320336018 1102 76-77 -0.022769111583830437 1102 78-79 -0.1846395461119208 1102 80-81 -0.06129973009179679 1102 82-83 0.06421661724339245 1102 84-85 0.021352092963155656 1102 86-87 0.05745635440069208 1102 88-89 -0.1286681199420272 1102 90-91 -0.08574666702478595 1102 92-93 -0.13484259942750398 1102 94-95 -0.29238814395052515 1102 96-97 -0.06703857199815388 1102 98-99 -0.07471993735531157 1102 100-101 0.6114481434709269 1103 1 -0.06544696271231842 1103 2 -0.06320970916968705 1103 3 -0.10578877132076059 1103 4 -0.056946006379071434 1103 5 -0.06612278437138741 1103 6 -0.22058322393662877 1103 7 -0.19234954197959553 1103 8 -0.3672268510288319 1103 9 -0.3221281621004124 1103 10-11 -0.23584926949120444 1103 12-13 -0.05722777402406365 1103 14-15 -0.25568232864205953 1103 16-17 -0.38724100244359505 1103 18-19 -0.3717549648236229 1103 20-21 -0.39278959997623986 1103 22-23 -0.44188958114856547 1103 24-25 -0.3125089495357187 1103 26-27 -0.2673046853835288 1103 28-29 -0.5011873596521781 1103 30-31 -0.501309685147568 1103 32-33 -0.44068557291611654 1103 34-35 -0.17149173860515532 1103 36-37 -0.1286601408686181 1103 38-39 -0.14874275940929493 1103 40-41 -0.03839691368491316 1103 42-43 -0.07036066058181945 1103 44-45 -0.21807578496292734 1103 46-47 0.05319408961332073 1103 48-49 0.1340905939515551 1103 50-51 0.3828302302460571 1103 52-53 0.09077470257631859 1103 54-55 -0.09784419882231532 1103 56-57 -0.17847082736938802 1103 58-59 -0.22585958393306527 1103 60-61 -0.14013476017411364 1103 62-63 0.10082988253315506 1103 64-65 -0.2235007724052167 1103 66-67 0.16135367128521594 1103 68-69 -0.08619020672956523 1103 70-71 0.11130625510122627 1103 72-73 0.09101691137515999 1103 74-75 0.15788960230061377 1103 76-77 0.10553699037098951 1103 78-79 0.052029178740330195 1103 80-81 0.05523485081864621 1103 82-83 0.01633835778987347 1103 84-85 -0.12426566804757044 1103 86-87 -0.04094216234864234 1103 88-89 -0.01790946368842583 1103 90-91 0.049032541682016983 1103 92-93 0.13331212410841076 1103 94-95 0.03718815253676411 1103 96-97 0.6525161135404929 1103 98-99 0.8125494497351156 1103 100-101 1.2289547081782466 1104 1 -0.04299760712172329 1104 2 -0.1478548555328878 1104 3 -0.12279663791430195 1104 4 -0.09314476212048817 1104 5 -0.06758170327178448 1104 6 -0.06709681480521112 1104 7 -0.06764946656998916 1104 8 -0.22276602974961435 1104 9 -0.21907193052435048 1104 10-11 -0.20302038075466555 1104 12-13 -0.17123768010482365 1104 14-15 -0.09306000225554811 1104 16-17 -0.2724148730132825 1104 18-19 -0.3030936568096365 1104 20-21 -0.3757828045419487 1104 22-23 -0.5084578439853331 1104 24-25 -0.3305515559661103 1104 26-27 -0.6561761976890068 1104 28-29 -0.3276777706345584 1104 30-31 -0.1650170958557311 1104 32-33 -0.31128632187066074 1104 34-35 -0.2684466470853053 1104 36-37 -0.3850763847844405 1104 38-39 -0.3996446754989691 1104 40-41 -0.6737775179901249 1104 42-43 -0.5663866597591749 1104 44-45 -0.30601903066115455 1104 46-47 -0.06167917082405694 1104 48-49 0.139504232808072 1104 50-51 -0.0037232934394140216 1104 52-53 0.3789433201810297 1104 54-55 0.2492338929717519 1104 56-57 0.32864274121907755 1104 58-59 0.28826472195574127 1104 60-61 0.26394614234182967 1104 62-63 0.26286414249064904 1104 64-65 0.42799970747412885 1104 66-67 0.29863558326163187 1104 68-69 0.37212884448041805 1104 70-71 0.38204175588960254 1104 72-73 0.600875861124937 1104 74-75 0.46892639876321596 1104 76-77 0.8022628850031985 1104 78-79 0.4413773569126782 1104 80-81 0.5207788711792389 1104 82-83 0.5013635514554657 1104 84-85 0.3145015133468192 1104 86-87 0.3605872388304867 1104 88-89 0.18611209660498673 1104 90-91 0.1208152077545499 1104 92-93 0.8372137145656673 1104 94-95 1.0260588079148363 1104 96-97 0.7841037429365301 1104 98-99 1.2266971068955996 1104 100-101 1.9563382517444339 1105 1 -0.09354748884313224 1105 2 -0.09478018079901673 1105 3 -0.1911053677841963 1105 4 -0.08432788922448253 1105 5 -0.2054530298116788 1105 6 -0.2028243418728053 1105 7 -0.18201576626974258 1105 8 -0.08749626357729312 1105 9 -0.1659517640244914 1105 10-11 -0.2174071517491143 1105 12-13 -0.2219780585963136 1105 14-15 -0.11762413427265273 1105 16-17 -0.32373811475653014 1105 18-19 -0.26327130215582883 1105 20-21 -0.22884582201078274 1105 22-23 -0.31453497527457586 1105 24-25 -0.3443639934149303 1105 26-27 -0.2095522553725644 1105 28-29 -0.13798137130783772 1105 30-31 -0.1681332818260728 1105 32-33 -0.28732211251755047 1105 34-35 -0.333872336285566 1105 36-37 -0.02057645302208755 1105 38-39 0.14043641357391579 1105 40-41 -0.1098743813328511 1105 42-43 0.06316536007523865 1105 44-45 0.10004189418644671 1105 46-47 -0.13316663784252825 1105 48-49 0.2002044648160748 1105 50-51 0.052575610209018464 1105 52-53 0.20680674252658804 1105 54-55 0.11014139683855007 1105 56-57 0.33651708158846816 1105 58-59 0.16630982704172226 1105 60-61 0.11157005053944147 1105 62-63 0.04835056319850395 1105 64-65 0.08752227139006408 1105 66-67 0.06800085939665124 1105 68-69 0.3372821517318023 1105 70-71 0.5219204066038188 1105 72-73 0.6709502401623304 1105 74-75 0.308002119807707 1105 76-77 0.44635094568921474 1105 78-79 0.3435714816511002 1105 80-81 0.3270223317378793 1105 82-83 0.21246240506296132 1105 84-85 0.19619022456508972 1105 86-87 0.2111311497576125 1105 88-89 0.49369898587803007 1105 90-91 0.7984633971822639 1105 92-93 0.8634340311883584 1105 94-95 0.8060577843501022 1105 96-97 0.6751651795664984 1105 98-99 0.8331265150477236 1105 100-101 1.5222359180168397 1106 1 -0.26891796062421847 1106 2 -0.13609628906140614 1106 3 -0.12066179731573357 1106 4 -0.3992537948682653 1106 5 -0.2756642775506819 1106 6 -0.3691259299646106 1106 7 -0.262075838289789 1106 8 -0.2282962834906641 1106 9 -0.2411455045863775 1106 10-11 -0.43992669175010235 1106 12-13 -0.34805669984023524 1106 14-15 -0.23268196603780922 1106 16-17 -0.31627059845244787 1106 18-19 -0.2903659404791199 1106 20-21 -0.2634868004213686 1106 22-23 -0.25977941197492527 1106 24-25 -0.45645971037946964 1106 26-27 -0.5621144431888965 1106 28-29 -0.7177199252647952 1106 30-31 -0.4677984881784383 1106 32-33 -0.6089542626644189 1106 34-35 -0.5476092473889338 1106 36-37 -0.558310227768601 1106 38-39 -0.6793765666288678 1106 40-41 -0.8793355585453142 1106 42-43 -1.1063928948491792 1106 44-45 -0.7651486934241092 1106 46-47 -1.0473316865473308 1106 48-49 -1.1586076192847514 1106 50-51 -1.1763742910538184 1106 52-53 -0.9254408969085333 1106 54-55 -0.6633833538293814 1106 56-57 -0.9147731392840655 1106 58-59 -0.9183946252574415 1106 60-61 -0.842799276222479 1106 62-63 -0.5534379069563187 1106 64-65 -0.6461300908342622 1106 66-67 -0.9879142432402119 1106 68-69 -0.6967282364130973 1106 70-71 -0.5744956004322361 1106 72-73 -0.8407447200938876 1106 74-75 -0.5098132997837332 1106 76-77 -0.318511486065983 1106 78-79 -0.5830580963512588 1106 80-81 -0.7811384189964272 1106 82-83 -0.781370671055921 1106 84-85 -0.5693733239986578 1106 86-87 -0.39043951783834885 1106 88-89 -0.209151945902061 1106 90-91 -0.31086311581951165 1106 92-93 -0.19934288920791587 1106 94-95 -0.6868356293005178 1106 96-97 -0.46244662574705586 1106 98-99 -0.5747118878994542 1106 100-101 -0.17643527894305322 1107 1 -0.036908245605179246 1107 2 0.0882055765192149 1107 3 0.11774858799719112 1107 4 0.025929422330285945 1107 5 -0.019319288315685412 1107 6 -0.05266660895534869 1107 7 -0.1464713541085132 1107 8 -0.012627633517709569 1107 9 0.040364671790001694 1107 10-11 0.10135683620178781 1107 12-13 0.055208066246287046 1107 14-15 0.11071373008148555 1107 16-17 0.09724080821018788 1107 18-19 0.07223706947983999 1107 20-21 0.13623691546735728 1107 22-23 0.09418905663686417 1107 24-25 0.07322889992736492 1107 26-27 0.24811125070315398 1107 28-29 0.1778237339883404 1107 30-31 0.1114555447211174 1107 32-33 0.25165616082981046 1107 34-35 0.2913584788087462 1107 36-37 0.27279453746945137 1107 38-39 0.05936525355842548 1107 40-41 0.36629215259196 1107 42-43 0.23300967390367333 1107 44-45 0.42995508546375305 1107 46-47 0.2738499185519956 1107 48-49 0.5974311241263237 1107 50-51 0.34074630881679013 1107 52-53 0.6099656278509755 1107 54-55 0.5340046742126603 1107 56-57 0.5056294612865244 1107 58-59 0.5124190075869564 1107 60-61 0.38392073820156725 1107 62-63 0.5750461364430848 1107 64-65 0.6438767499815441 1107 66-67 0.6450786401901674 1107 68-69 0.5667412768814195 1107 70-71 0.7976938318330795 1107 72-73 0.755339575726321 1107 74-75 0.9143583781782318 1107 76-77 0.9347723706693039 1107 78-79 0.8181173042872167 1107 80-81 0.9907163966197245 1107 82-83 0.8378066810710969 1107 84-85 0.9177126475562787 1107 86-87 0.6079695947646648 1107 88-89 0.49506496629155805 1107 90-91 1.276952542754838 1107 92-93 1.4161555358135622 1107 94-95 1.2938907698849498 1107 96-97 1.6865413047205635 1107 98-99 2.2611244416051406 1107 100-101 2.843213506149276 1108 1 -0.26577330653616826 1108 2 -0.2999327670488299 1108 3 -0.14141651429686775 1108 4 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-0.7060015160108009 2316 84-85 -0.6647041962983558 2316 86-87 -1.2420255172914487 2316 88-89 -1.088219487602906 2316 90-91 -1.0524887652589463 2316 92-93 -0.8213007781879575 2316 94-95 -0.9351949402110975 2316 96-97 -1.308802928727637 2316 98-99 -1.0509218961028992 2316 100-101 -0.8012985548522664 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 9.0 5 16.0 6 26.0 7 104.0 8 233.0 9 400.0 10 701.0 11 1115.0 12 1667.0 13 2370.0 14 3156.0 15 4232.0 16 5675.0 17 7542.0 18 6985.0 19 4959.0 20 4385.0 21 4517.0 22 4602.0 23 5202.0 24 5889.0 25 6909.0 26 8576.0 27 10332.0 28 12655.0 29 15327.0 30 19202.0 31 23362.0 32 29258.0 33 37136.0 34 48581.0 35 66430.0 36 89961.0 37 106734.0 38 58365.0 39 3148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.48985863142406 21.0339558177323 16.192157163686595 21.284028387157047 2 16.1684454129687 21.66541340220452 39.229734213452886 22.93640697137388 3 19.526916285824328 25.35684690888295 30.934512902510473 24.18172390278225 4 13.336345614213519 18.22807857051155 37.080232823746556 31.355342991528378 5 12.983570039916101 38.38214746614468 34.36252621890829 14.271756275030928 6 30.925509406152724 37.97691436841307 16.8862241503784 14.211352075055814 7 27.460663891144392 33.94318126872602 20.6515196734732 17.944635166656383 8 26.4037534575927 35.66218214740057 19.3186687496978 18.615395645308926 9 26.259864247282692 16.915735610662143 20.277541111798413 36.54685903025675 10-11 24.591007159458854 27.73965179753437 27.14817112007016 20.52116992293661 12-13 25.747503043036033 25.071865672886133 27.806012705717574 21.374618578360263 14-15 22.36568534114081 26.41367397672763 26.605165005743896 24.615475676387664 16-17 20.967142240236626 29.683277005903957 27.607054072664884 21.74252668119453 18-19 21.02958315479178 27.514351406462843 30.87565671335702 20.58040872538836 20-21 21.639069838787396 26.46352666952333 31.494313625124427 20.403089866564848 22-23 21.487577124535544 26.495576150813534 31.235218057874768 20.781628666776157 24-25 21.508757596725385 26.688536343401164 31.236901119605175 20.565804940268272 26-27 20.950639792785335 27.165412424376566 31.028428658248362 20.855519124589737 28-29 20.889370571071282 27.415756565235515 31.115798249270533 20.579074614422677 30-31 21.112685801939094 27.001825715071735 31.002475969754823 20.883012513234352 32-33 20.907285662598976 27.092687660374782 30.83755858525061 21.16246809177564 34-35 21.527609888506703 27.125367017525136 30.720012938357726 20.627010155610428 36-37 21.76770312471343 26.8091608908126 30.559533952351497 20.863602032122476 38-39 21.2896895364764 27.12681394113856 30.69471434204592 20.888782180339117 40-41 21.517207113101517 26.95315593651863 30.75063378831595 20.7790031620639 42-43 21.280434626730276 27.206942955241654 30.41611707440715 21.096505343620922 44-45 21.359032287645995 27.486265453969473 30.341050244679167 20.813652013705365 46-47 21.895169290741237 27.714512469008802 29.54522339508509 20.845094845164873 48-49 21.676667772651715 27.96727729268199 29.24227241578813 21.113782518878164 50-51 21.776623255364168 28.105330903500857 29.192919917234526 20.92512592390045 52-53 22.03155558741978 27.995621633067508 29.080126115641576 20.892696663871135 54-55 21.72351929999483 28.20486955767823 29.183833055169757 20.88777808715718 56-57 21.807802029788235 28.39969521497515 28.79334808350576 20.999154671730853 58-59 21.601471904923077 27.84673048033653 29.084544498113434 21.467253116626956 60-61 21.41289867565238 28.170356154973515 29.214344903937693 21.20240026543641 62-63 21.56570801661312 28.06323122145767 29.37407359858078 20.996987163348432 64-65 21.345622550090283 28.31866374775538 29.116973758142745 21.218739944011592 66-67 21.248584867123398 28.278898305508637 28.94949205274715 21.52302477462081 68-69 21.616144269357935 28.101412715271096 28.936487002452623 21.34595601291835 70-71 21.576045364283132 28.071734523573323 28.65271013576939 21.69950997637416 72-73 21.535779727794292 27.983033411308057 28.655044375565847 21.826142485331804 74-75 21.435907610788856 28.321164718965864 28.48606208744396 21.75686558280132 76-77 21.592051580030244 27.980449074390556 28.746579921869657 21.68091942370954 78-79 21.57454478155684 28.263142186882572 28.540166431297486 21.622146600263104 80-81 21.425320165997796 28.356511778740746 28.500234257636713 21.717933797624745 82-83 21.516409329979798 28.36291850731661 28.442449590715036 21.678222571988552 84-85 21.423825518182554 28.440496228102862 28.291104258421427 21.844573995293153 86-87 21.576128729990145 28.275230214399926 28.172356931941906 21.976284123668023 88-89 21.394474854401793 28.127756278688228 28.210455060048318 22.26731380686166 90-91 21.195476817564472 28.346927392466863 28.230371154081844 22.227224635886817 92-93 21.227152828030533 28.262130968776784 28.409943542766435 22.100772660426244 94-95 21.017911955809506 28.0531439709087 28.339505174509434 22.589438898772357 96-97 21.21965696678877 28.02496636193722 28.175441463101503 22.579935208172508 98-99 20.950552464540397 28.333752940725315 27.880410225971087 22.835284368763205 100-101 21.1817341227458 28.201652029852266 28.04331930100144 22.573294546400493 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 166.5 2 134.0 3 183.5 4 168.5 5 151.5 6 269.5 7 591.0 8 954.5 9 1140.0 10 1028.0 11 883.5 12 895.5 13 972.0 14 1059.0 15 1085.0 16 1131.0 17 1204.0 18 1279.5 19 1406.5 20 1566.5 21 1791.0 22 2128.0 23 2555.0 24 3030.5 25 3687.0 26 4763.0 27 6047.0 28 6963.5 29 7977.0 30 9234.5 31 10606.5 32 12413.5 33 13487.0 34 14055.0 35 14932.5 36 16357.0 37 17774.0 38 18468.0 39 18601.0 40 18661.0 41 19576.5 42 19882.5 43 19715.5 44 20424.0 45 21126.0 46 21069.5 47 20950.0 48 21605.0 49 21398.5 50 20134.5 51 19118.5 52 18195.5 53 17969.0 54 18020.5 55 16541.0 56 14386.0 57 12753.0 58 11667.5 59 10199.5 60 8074.0 61 6669.0 62 5605.5 63 4059.0 64 2663.0 65 1905.0 66 1420.5 67 1162.5 68 952.0 69 749.0 70 605.0 71 386.0 72 255.0 73 194.5 74 123.0 75 81.5 76 57.0 77 41.0 78 33.0 79 26.0 80 17.5 81 13.0 82 9.5 83 5.0 84 2.5 85 2.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05685541218506521 2 0.0 3 0.0 4 0.0 5 0.0015005827262920435 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.667314140324493E-4 12-13 0.006169062319200624 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010837541912109203 24-25 3.334628280648986E-4 26-27 4.1682853508112313E-4 28-29 0.0028344340385516374 30-31 3.334628280648986E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.1682853508112313E-4 40-41 8.336570701622464E-5 42-43 0.01333851312259594 44-45 3.334628280648986E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.5009712104867387E-4 84-85 4.1682853508112313E-4 86-87 0.0 88-89 0.0 90-91 0.009420324892833383 92-93 0.0035013596946814347 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.013421878829612165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 599767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.10803014829337 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4271737335795 58.18276524130127 2 9.059610891393953 12.340645034457324 3 2.378618606969094 4.860090833842278 4 0.9925832667983208 2.704115642391662 5 0.5340220722336674 1.8185595697772359 6 0.3375882217546918 1.3795481270986374 7 0.2222016584927391 1.0593602078936983 8 0.17179847188621408 0.93606844021256 9 0.12840699929830077 0.7870993001514498 >10 0.6783360990546299 8.318821322302272 >50 0.044150712735496304 2.0948886049591073 >100 0.022565888980020977 2.9657510476602713 >500 0.0019622512156445425 0.8678576405427243 >1k 9.811256078222713E-4 1.6844289874094878 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3656 0.6095700497026345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3175 0.5293722395530265 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2201 0.3669758422854208 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1051 0.17523471614810418 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 798 0.13305166839789453 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 792 0.1320512799136998 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 677 0.11287716729996816 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 653 0.10887561336318938 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 603 0.10053904266156691 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 1.6673141403244926E-4 22-23 0.0 0.0 0.0 8.336570701622463E-5 1.6673141403244926E-4 24-25 0.0 0.0 0.0 5.001942420973478E-4 1.6673141403244926E-4 26-27 0.0 0.0 0.0 6.66925656129797E-4 1.6673141403244926E-4 28-29 0.0 0.0 0.0 0.0021675083824218406 1.6673141403244926E-4 30-31 0.0 0.0 0.0 0.007502913631460217 1.6673141403244926E-4 32-33 0.0 0.0 0.0 0.015255924383969107 1.6673141403244926E-4 34-35 0.0 0.0 0.0 0.02517644351889984 1.6673141403244926E-4 36-37 0.0 0.0 0.0 0.04168285350811232 1.6673141403244926E-4 38-39 0.0 0.0 0.0 0.07044402242870981 1.6673141403244926E-4 40-41 0.0 0.0 0.0 0.10003884841946956 1.6673141403244926E-4 42-43 0.0 0.0 0.0 0.13663639379959217 1.6673141403244926E-4 44-45 0.0 0.0 0.0 0.18498850386900245 1.6673141403244926E-4 46-47 0.0 0.0 0.0 0.24926346397851165 1.6673141403244926E-4 48-49 0.0 0.0 0.0 0.3232922118089191 1.6673141403244926E-4 50-51 0.0 0.0 0.0 0.40940898715667917 1.6673141403244926E-4 52-53 0.0 0.0 0.0 0.49869365937105575 1.6673141403244926E-4 54-55 0.0 0.0 0.0 0.6009833818799635 1.6673141403244926E-4 56-57 0.0 0.0 0.0 0.741454598202302 1.6673141403244926E-4 58-59 0.0 0.0 0.0 0.8910126765894089 1.6673141403244926E-4 60-61 0.0 0.0 0.0 1.0675812440497727 1.6673141403244926E-4 62-63 0.0 0.0 0.0 1.2592390044800732 1.6673141403244926E-4 64-65 0.0 0.0 0.0 1.452230616222633 1.6673141403244926E-4 66-67 0.0 0.0 0.0 1.68415401314177 1.6673141403244926E-4 68-69 0.0 0.0 0.0 1.9693480968442745 1.6673141403244926E-4 70-71 0.0 0.0 0.0 2.3019772678390105 1.6673141403244926E-4 72-73 0.0 0.0 0.0 2.6693699386595124 1.6673141403244926E-4 74-75 0.0 0.0 0.0 3.0452659115956697 1.6673141403244926E-4 76-77 0.0 0.0 0.0 3.456258847185657 1.6673141403244926E-4 78-79 0.0 0.0 0.0 3.947699690046301 1.6673141403244926E-4 80-81 0.0 0.0 0.0 4.542180546778999 1.6673141403244926E-4 82-83 0.0 0.0 0.0 5.111901788527878 1.6673141403244926E-4 84-85 0.0 0.0 0.0 5.726973974893584 1.6673141403244926E-4 86-87 8.336570701622463E-5 0.0 0.0 6.412656915102032 1.6673141403244926E-4 88-89 1.6673141403244926E-4 0.0 0.0 7.170784654707578 1.6673141403244926E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2035 0.0 44.37737 1 GGTAAGG 395 0.0 37.274426 3 AAGGTAA 390 0.0 35.343185 1 GTATCAA 3310 0.0 35.037582 1 TCGTACT 55 0.0025800224 34.541695 4 GTAAGGT 410 0.0 33.593906 4 AGGTAAG 410 0.0 32.435497 2 TAAGGTG 440 0.0 31.306023 5 ATACGAC 65 0.0058449805 29.227587 3 ACCGCCC 85 6.398289E-4 27.945124 8 ATCAACG 4190 0.0 27.658087 3 TCAACGC 4255 0.0 27.123957 4 TATCAAC 4275 0.0 27.108162 2 CAACGCA 4320 0.0 26.71807 5 GCCGCGC 145 7.867293E-7 26.210602 8 AACGCAG 4465 0.0 25.956787 6 GTATAGA 205 9.476935E-10 25.504168 1 GGTGTGC 700 0.0 25.11069 8 GTGTGCA 760 0.0 23.753355 9 CATACGA 120 1.7107492E-4 23.747416 2 >>END_MODULE