FastQCFastQC Report
Thu 26 May 2016
SRR937163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333457
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83370.3572810641036025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59280.25404367854218013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.17536213437830653No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37780.16190570471193597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44050.048.1250421
GTATCAA78350.043.1333051
ATCAACG98900.033.7710953
TATCAAC99350.033.6666762
TCAACGC99550.033.5079084
CAACGCA101400.032.8979845
AACGCAG105000.031.7611776
ACGCAGA121600.027.1921257
CGCAGAG122250.027.0475448
GTACATG77250.024.8579331
GCAGAGT132100.024.5986589
TACATGG75550.024.4001622
ACATGGG76750.022.90393
GAGTACT78900.022.36577612-13
ATAACGC1553.9022198E-521.4561823
AGAGTAC117850.021.14131710-11
CAGAGTA125200.020.79181310-11
AGTACTT83650.020.66986312-13
GTCTACG2308.967436E-820.66591
GTACTTT84550.020.53168514-15
Produced by FastQC (version 0.11.5)