##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937163_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2333457 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83122251663519 33.0 31.0 34.0 30.0 34.0 2 31.970985109217782 33.0 31.0 34.0 30.0 34.0 3 32.01057915359058 34.0 31.0 34.0 30.0 34.0 4 35.62403378335234 37.0 35.0 37.0 33.0 37.0 5 35.62363009046235 37.0 35.0 37.0 33.0 37.0 6 35.65807640766468 37.0 35.0 37.0 33.0 37.0 7 35.59344011910226 37.0 35.0 37.0 33.0 37.0 8 35.57660415426554 37.0 35.0 37.0 33.0 37.0 9 37.207229445410825 39.0 37.0 39.0 34.0 39.0 10-11 37.09553872216201 39.0 37.0 39.0 34.0 39.0 12-13 36.96020946604116 39.0 37.0 39.0 33.0 39.0 14-15 38.10832704438093 40.0 38.0 41.0 33.0 41.0 16-17 38.01736329403113 40.0 38.0 41.0 33.0 41.0 18-19 37.92655253557275 40.0 38.0 41.0 33.0 41.0 20-21 37.78429964640445 40.0 38.0 41.0 33.0 41.0 22-23 37.86107907709463 40.0 38.0 41.0 33.0 41.0 24-25 37.827591423368844 40.0 38.0 41.0 33.0 41.0 26-27 37.734959547144 40.0 38.0 41.0 33.0 41.0 28-29 37.592473527474475 40.0 37.0 41.0 32.5 41.0 30-31 37.341674819805974 39.5 37.0 41.0 32.0 41.0 32-33 37.094227148818256 39.0 36.5 41.0 31.0 41.0 34-35 37.11283151992944 39.5 37.0 41.0 31.0 41.0 36-37 36.97312485295423 39.5 36.0 41.0 31.0 41.0 38-39 36.824724646736584 39.0 36.0 41.0 31.0 41.0 40-41 36.59737162501816 39.0 36.0 41.0 30.0 41.0 42-43 36.33718684338301 39.0 35.5 41.0 30.0 41.0 44-45 36.10312724854154 39.0 35.0 40.5 29.5 41.0 46-47 35.73712650372387 39.0 35.0 40.0 28.5 41.0 48-49 35.40761603920706 39.0 35.0 40.0 27.0 41.0 50-51 34.44446265776485 37.5 34.0 39.5 25.0 40.5 52-53 34.552173234818554 38.0 34.0 39.5 26.0 40.5 54-55 34.84579552997977 38.0 34.0 40.0 26.0 41.0 56-57 34.56009817193974 38.0 34.0 40.0 24.5 41.0 58-59 34.266613226641844 38.0 34.0 40.0 24.0 41.0 60-61 33.91671412843691 37.5 33.5 40.0 23.0 41.0 62-63 33.51431802685886 37.0 33.0 40.0 22.0 41.0 64-65 33.06260111071256 36.0 32.0 39.0 20.5 41.0 66-67 32.618110383006844 36.0 32.0 39.0 19.0 40.5 68-69 32.08906892220426 35.0 31.0 39.0 17.0 40.0 70-71 31.606852408250937 35.0 31.0 38.0 12.5 40.0 72-73 31.097932595286736 35.0 30.5 37.5 8.0 39.5 74-75 30.529828490518575 34.5 30.0 37.0 7.0 39.0 76-77 29.986529856774734 34.0 29.0 36.0 2.0 39.0 78-79 29.426204125467066 34.0 29.0 36.0 2.0 38.0 80-81 28.828998777350513 34.0 28.5 35.0 2.0 37.0 82-83 28.239629013947976 33.5 27.0 35.0 2.0 37.0 84-85 27.630414230902907 33.0 26.0 35.0 2.0 36.0 86-87 27.00281813635306 33.0 25.5 35.0 2.0 36.0 88-89 26.404908468422605 32.0 24.5 35.0 2.0 35.0 90-91 25.79874130956774 32.0 22.5 34.5 2.0 35.0 92-93 25.082763899227626 31.5 19.5 34.0 2.0 35.0 94-95 24.241964390173038 31.0 12.0 34.0 2.0 35.0 96-97 23.32853551618907 31.0 2.0 34.0 2.0 35.0 98-99 22.09447484997581 30.5 2.0 34.0 2.0 35.0 100-101 19.006339521148234 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06294133490507292 1101 2 0.11551731143411104 1101 3 0.037885432716894485 1101 4 -0.07579863685941746 1101 5 -0.05039051232572689 1101 6 -0.07758993714138285 1101 7 -0.04041141328563924 1101 8 -0.049005123130342554 1101 9 0.02224308375443229 1101 10-11 0.11366910726076895 1101 12-13 0.131087873213815 1101 14-15 0.27008847270474234 1101 16-17 0.2401556247177865 1101 18-19 0.23861967197574074 1101 20-21 0.30338728357261147 1101 22-23 0.21486480041387068 1101 24-25 0.1599575037255221 1101 26-27 0.03746623733742638 1101 28-29 0.14747179508638197 1101 30-31 0.1673376186862967 1101 32-33 0.08559057244495705 1101 34-35 0.22419812956079 1101 36-37 0.12158025621509694 1101 38-39 0.29071332248236814 1101 40-41 0.28894275053276175 1101 42-43 0.43141113695059374 1101 44-45 0.38060811894671076 1101 46-47 0.6058376634244382 1101 48-49 0.4082061773203236 1101 50-51 0.4441219776546106 1101 52-53 0.504642503704865 1101 54-55 0.5308970039654142 1101 56-57 0.5683687517603815 1101 58-59 0.5392784702040743 1101 60-61 0.5924794727314762 1101 62-63 0.5814828521599367 1101 64-65 0.5320500034820697 1101 66-67 0.6158687293345722 1101 68-69 0.6238435261137099 1101 70-71 0.5423180774401111 1101 72-73 0.5242638873264838 1101 74-75 0.7380890862598442 1101 76-77 0.6834438099197158 1101 78-79 0.8693900015647706 1101 80-81 0.9200450977422925 1101 82-83 0.7185660511222451 1101 84-85 0.9809266182589766 1101 86-87 1.0849958774665147 1101 88-89 1.1317767301275268 1101 90-91 1.1951988423569055 1101 92-93 1.4077781144243318 1101 94-95 1.5419441901806366 1101 96-97 1.5797571651309887 1101 98-99 1.9149390480564676 1101 100-101 1.8358399321121261 1102 1 0.10202246732691833 1102 2 0.21251266444532035 1102 3 0.030229483223944698 1102 4 0.09677764884293794 1102 5 0.14390963839806403 1102 6 0.01808727204953442 1102 7 -0.034926698002337275 1102 8 -0.03367283743114058 1102 9 0.1486884721813979 1102 10-11 0.06585635538144885 1102 12-13 0.11452736190322099 1102 14-15 0.31228409415086134 1102 16-17 0.3236263139423343 1102 18-19 0.2771208171771917 1102 20-21 0.36906115997908984 1102 22-23 0.33843955248385527 1102 24-25 0.35555565777264775 1102 26-27 0.2843075278577416 1102 28-29 0.24645780724040378 1102 30-31 0.2831985604224485 1102 32-33 0.36379499937542903 1102 34-35 0.35445903418721514 1102 36-37 0.40816578901544176 1102 38-39 0.4227665836080021 1102 40-41 0.5152645186576308 1102 42-43 0.5354181405252589 1102 44-45 0.4683283052989964 1102 46-47 0.4891953787416199 1102 48-49 0.3425716955289104 1102 50-51 0.2766846210017704 1102 52-53 0.43580730792545097 1102 54-55 0.5894049506471646 1102 56-57 0.6742262188914907 1102 58-59 0.7521680041579017 1102 60-61 0.6029192919417099 1102 62-63 0.5770546019663101 1102 64-65 0.5553815135013025 1102 66-67 0.7705738448829251 1102 68-69 0.93409581710754 1102 70-71 0.8588619346771758 1102 72-73 0.9835735833655157 1102 74-75 0.9873802786481711 1102 76-77 0.9450386792438401 1102 78-79 0.9176807578547113 1102 80-81 0.9545466061621006 1102 82-83 1.0564862143892881 1102 84-85 1.1508619874534922 1102 86-87 1.049055923920644 1102 88-89 1.1145374495992613 1102 90-91 1.1134086289247627 1102 92-93 1.2700245509844272 1102 94-95 1.0440785904251975 1102 96-97 1.0503190465862922 1102 98-99 1.3088686037876371 1102 100-101 1.4845719868800948 1103 1 0.15832625052622262 1103 2 0.23052825613920902 1103 3 0.16058281966855148 1103 4 0.10653190128207513 1103 5 0.0748161092208548 1103 6 0.07689839789534858 1103 7 0.06857089009193373 1103 8 0.06091733846259473 1103 9 0.16997909907701825 1103 10-11 0.11991342365931956 1103 12-13 0.17749623947515403 1103 14-15 0.16103062269548474 1103 16-17 0.1642029927576374 1103 18-19 0.03829173320170298 1103 20-21 0.09248462706734983 1103 22-23 0.08152157526679815 1103 24-25 0.1280990565860236 1103 26-27 0.08804475754891428 1103 28-29 -0.030469493813598092 1103 30-31 0.06676342404759339 1103 32-33 0.2618982031577275 1103 34-35 0.3639299239290992 1103 36-37 0.3405313953496574 1103 38-39 0.4659222577074189 1103 40-41 0.4623364541083035 1103 42-43 0.342220843938307 1103 44-45 0.5944555851669975 1103 46-47 0.4616972050344401 1103 48-49 0.4614290308302742 1103 50-51 0.4218820954711475 1103 52-53 0.38296353765759505 1103 54-55 0.5638454954470546 1103 56-57 0.5830483374711051 1103 58-59 0.6483236452791203 1103 60-61 0.5578407888322658 1103 62-63 0.6864459942560046 1103 64-65 0.48731885291994814 1103 66-67 0.7842584493593634 1103 68-69 0.7059320333685974 1103 70-71 0.8233073862336902 1103 72-73 0.8413488938852574 1103 74-75 0.6388534049117105 1103 76-77 0.6139144523578075 1103 78-79 0.6418191024089595 1103 80-81 0.580331712207002 1103 82-83 0.6859444924674776 1103 84-85 0.8739505758965329 1103 86-87 1.086553787832596 1103 88-89 0.9950544278766209 1103 90-91 0.858324239706036 1103 92-93 1.1217719459563504 1103 94-95 1.164804540572149 1103 96-97 1.2162736505930418 1103 98-99 1.3955024388226818 1103 100-101 1.4803350874706034 1104 1 0.1277143874684974 1104 2 0.1315772393678145 1104 3 0.033575648110439005 1104 4 0.11005962823898585 1104 5 0.12991038320046755 1104 6 0.13602557345845412 1104 7 0.12871852851433374 1104 8 0.07789919566454273 1104 9 0.134889032357556 1104 10-11 0.12634001354349067 1104 12-13 0.16561578634514262 1104 14-15 0.31920302327115735 1104 16-17 0.13875149609563664 1104 18-19 0.20498939807117722 1104 20-21 0.2214775546225738 1104 22-23 0.21760580946960317 1104 24-25 0.18092459586878817 1104 26-27 0.2573884957602388 1104 28-29 0.2614576740631591 1104 30-31 0.2015493746304955 1104 32-33 0.21692911539489046 1104 34-35 0.4082143205564108 1104 36-37 0.4308683503653512 1104 38-39 0.5205560591995777 1104 40-41 0.4209863180297262 1104 42-43 0.4540874414279017 1104 44-45 0.4815366163907413 1104 46-47 0.5386884982309184 1104 48-49 0.5849750229598598 1104 50-51 0.40581951449909326 1104 52-53 0.5542004418018465 1104 54-55 0.7389443306630312 1104 56-57 0.7604466021785683 1104 58-59 0.7362917914652272 1104 60-61 0.6258098856973504 1104 62-63 0.6688322389425707 1104 64-65 0.7359866887194855 1104 66-67 0.8514380275613078 1104 68-69 0.7572506959048653 1104 70-71 0.7127933318609259 1104 72-73 0.8436005871557661 1104 74-75 0.7853052484704044 1104 76-77 0.7874863858871457 1104 78-79 0.8634198435340181 1104 80-81 0.807319212801179 1104 82-83 1.012998843998222 1104 84-85 1.010994641889834 1104 86-87 1.078767271899114 1104 88-89 1.016006785712186 1104 90-91 0.9292499481894296 1104 92-93 0.9745377643948707 1104 94-95 1.1492490558118398 1104 96-97 1.0954407344459618 1104 98-99 1.2842515576747466 1104 100-101 1.2914401304180352 1105 1 0.03127153676190986 1105 2 -0.08960433902401022 1105 3 0.0018254663130861104 1105 4 0.02975316659134819 1105 5 -0.028124398246752946 1105 6 0.0026824601821076044 1105 7 0.026290269978950676 1105 8 0.03414976814271142 1105 9 0.00585116164757693 1105 10-11 0.00741642343185589 1105 12-13 0.04178444301310691 1105 14-15 0.08304091895823973 1105 16-17 0.05087596851112863 1105 18-19 0.044309224744949915 1105 20-21 0.10918850830348248 1105 22-23 0.08529951488737453 1105 24-25 0.1294948116209511 1105 26-27 0.1595264678053212 1105 28-29 0.0502704013873867 1105 30-31 0.10120519507156445 1105 32-33 0.1797893682798133 1105 34-35 0.2559058714920255 1105 36-37 0.18313526051744589 1105 38-39 0.15099966748991278 1105 40-41 0.03966933808440132 1105 42-43 0.1555692578672705 1105 44-45 0.2376215294536621 1105 46-47 0.23005174371637338 1105 48-49 0.3191267080150979 1105 50-51 0.24895517306039494 1105 52-53 0.16249976760607154 1105 54-55 0.41741392051952175 1105 56-57 0.24277855618843347 1105 58-59 0.13619934722589022 1105 60-61 0.21453826359765316 1105 62-63 0.023116257171722054 1105 64-65 0.18340057611450078 1105 66-67 0.21930226168942113 1105 68-69 0.1319003599441828 1105 70-71 0.2639450700545396 1105 72-73 0.4780896905831078 1105 74-75 0.38897600431113233 1105 76-77 0.400150243869966 1105 78-79 0.351840742507207 1105 80-81 0.34959859910044955 1105 82-83 0.4549629460247395 1105 84-85 0.5142723184565732 1105 86-87 0.6601662234809531 1105 88-89 0.5684815558370069 1105 90-91 0.42921329924864793 1105 92-93 0.6025448125702901 1105 94-95 0.6784422890837405 1105 96-97 0.6978088246215854 1105 98-99 0.9274459992761841 1105 100-101 0.7498614677311615 1106 1 -0.09195392082096276 1106 2 -0.5696152663762284 1106 3 -0.1840961243136725 1106 4 0.012835894067457332 1106 5 -0.06465368231406643 1106 6 -0.003095883199819127 1106 7 -4.947838315132458E-4 1106 8 -0.0360175485065497 1106 9 -0.0716121861918495 1106 10-11 -0.42222390704184676 1106 12-13 -0.23841771300295278 1106 14-15 -0.16774839112336082 1106 16-17 -0.08621795163151091 1106 18-19 -0.024881544115686438 1106 20-21 -0.16618886195950466 1106 22-23 -0.17068652688163155 1106 24-25 -0.13925891936158052 1106 26-27 -0.09524559234908025 1106 28-29 0.046116265013587565 1106 30-31 0.12506380952377327 1106 32-33 0.02593646483062173 1106 34-35 0.06347598585823988 1106 36-37 0.019203729597649044 1106 38-39 0.11071612968093092 1106 40-41 0.31499409517002874 1106 42-43 0.12037407759405738 1106 44-45 0.11275910504294018 1106 46-47 0.30661397847232763 1106 48-49 0.3480940844724998 1106 50-51 0.2281896625720421 1106 52-53 0.19615826086809562 1106 54-55 0.37073760060891203 1106 56-57 0.09308748997340643 1106 58-59 -0.022310328314937067 1106 60-61 0.10029155950967805 1106 62-63 0.19213080561462448 1106 64-65 0.26190629442489666 1106 66-67 0.46093879007411687 1106 68-69 0.3153269842355044 1106 70-71 0.291674968841626 1106 72-73 0.3698675325659515 1106 74-75 0.21908001651559061 1106 76-77 0.24268659249297642 1106 78-79 0.3991665203784187 1106 80-81 0.40134731271950486 1106 82-83 0.3347182272972624 1106 84-85 0.33624512149105 1106 86-87 0.4480465869615635 1106 88-89 0.6548631745016387 1106 90-91 0.4022470784824108 1106 92-93 0.6148086294314616 1106 94-95 0.501304625805826 1106 96-97 0.42225045603479927 1106 98-99 0.3050145172596572 1106 100-101 0.18546670476589355 1107 1 0.001294121650733615 1107 2 0.10547988283227738 1107 3 -0.02422121219033002 1107 4 0.03595412150436772 1107 5 -0.05667266367209578 1107 6 -0.03888592465335705 1107 7 0.05270332045687098 1107 8 -0.021990009780218145 1107 9 0.12332248205861163 1107 10-11 0.04226695378576295 1107 12-13 -0.025565903349850316 1107 14-15 -0.04307717103173303 1107 16-17 0.09061594330437117 1107 18-19 0.1347779186852165 1107 20-21 0.1331935772540973 1107 22-23 0.07909166031382853 1107 24-25 0.027831878437268642 1107 26-27 0.07323183850108705 1107 28-29 0.017777255050184237 1107 30-31 -0.028619737541752954 1107 32-33 0.05734661246340522 1107 34-35 0.18595665299357478 1107 36-37 0.08164251896033647 1107 38-39 0.12388641646186471 1107 40-41 0.05573462267239648 1107 42-43 0.0487821767916472 1107 44-45 0.18586763574522536 1107 46-47 0.15548067261308063 1107 48-49 0.1674790690789294 1107 50-51 0.2720289028328864 1107 52-53 0.3069516355741655 1107 54-55 0.30279894457633816 1107 56-57 0.5135021465877898 1107 58-59 0.5481779628780075 1107 60-61 0.3438817148998581 1107 62-63 0.36529054719665055 1107 64-65 0.2899889469666519 1107 66-67 0.4741518030919778 1107 68-69 0.48827127649509094 1107 70-71 0.488673223429398 1107 72-73 0.6650703761333823 1107 74-75 0.6485668774401745 1107 76-77 0.6330562198959448 1107 78-79 0.5774291685566482 1107 80-81 0.4896503235556082 1107 82-83 0.45437865977162417 1107 84-85 0.3756329545132786 1107 86-87 0.3550312661208572 1107 88-89 0.3776653489943804 1107 90-91 0.4135907428784229 1107 92-93 0.4999859584659099 1107 94-95 0.6644164291560521 1107 96-97 0.816313024214967 1107 98-99 1.1711768527181938 1107 100-101 1.0575152611645287 1108 1 0.07922859807449356 1108 2 0.08420737823950475 1108 3 0.04721540752797537 1108 4 0.04301474612163503 1108 5 -0.009884543647601163 1108 6 0.05031248318497461 1108 7 -0.001337708324513187 1108 8 -0.0339030532584772 1108 9 0.04629247593491215 1108 10-11 0.14078410014216303 1108 12-13 0.05973599499674975 1108 14-15 0.08276391898664315 1108 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2316 20-21 -0.0999189396552751 2316 22-23 -0.41601457029013744 2316 24-25 -0.17191448107574558 2316 26-27 -0.24215866838202516 2316 28-29 -0.0242484869725601 2316 30-31 -0.05684445306653885 2316 32-33 0.08062981450048312 2316 34-35 0.01201299102174147 2316 36-37 -0.13909532618966125 2316 38-39 -0.1916254399875683 2316 40-41 -0.3436715601874738 2316 42-43 -0.24622160294893547 2316 44-45 -0.1982097096832689 2316 46-47 -0.4871548012470299 2316 48-49 -0.4406016184359842 2316 50-51 -0.1320979770907158 2316 52-53 -0.04257390751487833 2316 54-55 -0.0016740800118739685 2316 56-57 -0.2099581064558933 2316 58-59 -0.19037240713870318 2316 60-61 -0.20704432154480656 2316 62-63 -0.035199824411527914 2316 64-65 -0.02367282593061759 2316 66-67 0.10549182059815365 2316 68-69 0.13265537771255964 2316 70-71 0.1940145936848232 2316 72-73 0.24700222144252493 2316 74-75 0.4038296688780676 2316 76-77 0.4540725722473411 2316 78-79 0.2953361869381226 2316 80-81 0.3540063751192264 2316 82-83 0.651588614414738 2316 84-85 0.8958734653750575 2316 86-87 1.0343083409995053 2316 88-89 1.0095613862347754 2316 90-91 1.1680677461409381 2316 92-93 1.2395857038307554 2316 94-95 1.1793105964195192 2316 96-97 0.8559426096371006 2316 98-99 1.491109016321282 2316 100-101 2.214117871174871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9797.0 3 1747.0 4 1735.0 5 1855.0 6 2131.0 7 2514.0 8 3097.0 9 3815.0 10 4877.0 11 6520.0 12 8575.0 13 10437.0 14 12323.0 15 15124.0 16 18152.0 17 22894.0 18 20670.0 19 16551.0 20 16814.0 21 18102.0 22 20138.0 23 22399.0 24 25599.0 25 29337.0 26 34039.0 27 40252.0 28 47726.0 29 57227.0 30 69614.0 31 85913.0 32 108204.0 33 139899.0 34 185307.0 35 250091.0 36 336795.0 37 403238.0 38 254881.0 39 25063.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93797307677594 19.024773544280784 14.881277582936331 23.155975796006953 2 15.072724561871627 19.829221562958274 40.75119138004153 24.346862495128565 3 18.99095588971419 23.99471334823713 29.78691407601109 27.227416686037593 4 12.57559977107384 16.35816632207436 37.62771828332878 33.438515623523024 5 13.570994600716869 37.14308057332257 34.79018590274072 14.49573892321984 6 32.0387536332605 36.27517725500539 17.432133679073836 14.253935432660278 7 28.46602677378701 32.442191007423226 20.90324376379345 18.188538454996312 8 26.21085570329262 34.9072306208751 19.93239078470073 18.94952289113155 9 25.862480765744593 16.67398377406376 20.51104052703793 36.952494933153716 10-11 24.577072898081195 26.63146516632462 27.707143737860594 21.08431819773359 12-13 25.432404991185976 23.80633669176379 28.198228946481553 22.56302937056869 14-15 22.462254605808987 25.29247995814551 26.37971311374186 25.865552322303646 16-17 21.89318004104908 28.53022854463889 27.0923295085428 22.48426190576923 18-19 21.878311599043748 27.120115904758652 29.473169753358814 21.528402742838786 20-21 22.425769343263827 26.246791137117498 29.71257779761089 21.614861722007785 22-23 22.12035569035638 26.076980347010476 29.64000406292204 22.162659899711105 24-25 21.993905918182797 26.23283395420183 29.731830764998605 22.041429362616764 26-27 21.71477359793577 26.597883375192833 29.530832872363455 22.15651015450794 28-29 21.75158503136774 26.794906680680903 29.522228023307335 21.931280264644023 30-31 21.91201026541176 26.484148023663373 29.423983930008248 22.179857780916628 32-33 21.752045831083574 26.47606750265353 29.416389930185517 22.355496736077374 34-35 22.20610476841338 26.597636046320332 29.246914476456464 21.94934470880982 36-37 22.20590354379704 26.334187970129875 29.252458736161184 22.2074497499119 38-39 21.889829584056606 26.662158830991807 29.25707031622417 22.190941268727414 40-41 22.191452740394897 26.563703778140223 29.157100857766405 22.08774262369847 42-43 21.86222993688801 26.47731423688696 29.135752944771586 22.524702881453443 44-45 22.05941784682779 26.667854880444935 29.108517560153324 22.16420971257395 46-47 22.28415672093589 26.893944681599525 28.539180770873685 22.282717826590897 48-49 22.168988415203934 26.94553421263613 28.49244306878436 22.393034303375572 50-51 22.263632915150016 27.172693830611056 28.320403201866856 22.24327005237208 52-53 22.35235785530347 27.082612316904793 28.252887771496926 22.312142056294814 54-55 22.18037749173021 27.133426200714677 28.263341309962716 22.422854997592406 56-57 22.141807596251155 27.36220916262929 28.175373885065707 22.320609356053847 58-59 22.23746867719481 27.081368333430312 28.220959135373562 22.46020385400132 60-61 22.144630201040965 27.16890442733288 28.36358155360631 22.322883818019847 62-63 22.044334182059764 27.148481031629913 28.56637952543753 22.240805260872794 64-65 21.97111289699584 27.096640244113217 28.409723562649663 22.522523296241275 66-67 21.970462134641547 27.196187354852896 28.178485420016212 22.65486509048934 68-69 22.09317106268949 26.968182872259106 28.451271568568508 22.487374496482897 70-71 22.092001316691036 26.940911449951248 28.307440055879923 22.659647177477797 72-73 22.10379918778251 27.023999989693216 28.139494585395674 22.7327062371286 74-75 22.033036477723805 27.28867772921563 27.998786352000003 22.679499441060553 76-77 22.150364495129256 27.067451678034814 28.113756496723557 22.66842733011237 78-79 22.15190987955823 27.058703015382292 28.138993807397966 22.650393297661516 80-81 22.14988917410767 27.154147413683145 27.935619196994367 22.76034421521482 82-83 22.197917865541626 27.11526635208188 27.982428715968418 22.70438706640808 84-85 22.17308981733294 27.156355195225807 27.95844480623414 22.712110181207112 86-87 22.212193222094452 27.091848083263415 27.967963785671774 22.727994908970363 88-89 22.248859614239986 26.985658323162692 27.868936587594767 22.896545475002554 90-91 22.231216538816152 26.932820184906923 27.965508489752132 22.870454786524785 92-93 22.180502711279107 26.801562456047602 28.128481969290707 22.889452863382584 94-95 22.12639538027112 26.80216600250406 28.02256614052563 23.0488724766992 96-97 22.24801434089517 26.736866562505636 27.926694456302613 23.088424640296576 98-99 22.02343998799161 26.89246539909976 27.82294406197636 23.26115055093227 100-101 22.668431607470293 26.022871228029494 28.145008567048425 23.163688597451788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3392.0 1 1907.0 2 423.0 3 435.0 4 379.5 5 340.5 6 557.5 7 1166.0 8 1981.0 9 2391.0 10 2214.5 11 1963.5 12 2051.0 13 2241.0 14 2343.0 15 2532.5 16 2724.5 17 2887.5 18 3116.5 19 3419.0 20 3899.0 21 4634.0 22 5562.0 23 6953.5 24 8768.5 25 11248.5 26 14786.0 27 18930.5 28 22374.5 29 25720.5 30 29477.5 31 34082.5 32 40136.5 33 44042.5 34 47234.5 35 51613.5 36 56631.5 37 61551.0 38 64712.5 39 66844.5 40 69318.5 41 72572.0 42 74633.5 43 77077.5 44 80735.0 45 83665.5 46 84925.0 47 86211.0 48 86885.0 49 85988.0 50 84248.0 51 80938.5 52 79151.0 53 79070.0 54 77207.5 55 71244.5 56 64823.5 57 59696.5 58 54734.0 59 48517.5 60 39825.0 61 32452.5 62 26473.0 63 19877.0 64 14318.0 65 10905.0 66 8193.0 67 6044.0 68 4650.0 69 3609.0 70 2718.0 71 1911.0 72 1368.0 73 1031.5 74 744.0 75 508.0 76 361.5 77 259.5 78 178.0 79 127.5 80 76.0 81 43.0 82 35.5 83 27.5 84 22.5 85 20.5 86 12.5 87 8.5 88 6.5 89 3.0 90 5.5 91 7.0 92 5.0 93 4.0 94 3.5 95 3.5 96 2.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3027268126217882 2 0.26201468465028493 3 0.26081474824691436 4 0.2600862154305822 5 0.2618861200356381 6 0.22773078741112435 7 0.22965925663082712 8 0.23021636996096348 9 0.2390873283715963 10-11 0.24898680369940396 12-13 0.2414229188710141 14-15 0.23105203995616805 16-17 0.2257808907556471 18-19 0.21399580107968563 20-21 0.22027403976160692 22-23 0.2178527395190912 24-25 0.2199526282249898 26-27 0.2156885685058692 28-29 0.22920928047956315 30-31 0.2417014755360823 32-33 0.23270195251080267 34-35 0.23175914533672573 36-37 0.2218810974446926 38-39 0.2108031131492888 40-41 0.20788898188396016 42-43 0.22008119283963662 44-45 0.21564571363432025 46-47 0.22629514921423452 48-49 0.22980924868124847 50-51 0.24384421911352985 52-53 0.2577077700596154 54-55 0.23167343559362782 56-57 0.21001029802563323 58-59 0.20066793602796196 60-61 0.2036677770363885 62-63 0.20870322444339023 64-65 0.201482178587392 66-67 0.20506056036172937 68-69 0.20728901368227484 70-71 0.2096674590532416 72-73 0.20971031392479056 74-75 0.21170306545181677 76-77 0.22147397616497752 78-79 0.2226310576967992 80-81 0.21556000389122235 82-83 0.20934604751662444 84-85 0.1972395463040459 86-87 0.19948942706036582 88-89 0.20823182085635178 90-91 0.2068176100952364 92-93 0.2118530575022381 94-95 0.2072247313749514 96-97 0.21474576133179227 98-99 0.2179384492621891 100-101 0.2042891726738483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2333457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.07491533332679 #Duplication Level Percentage of deduplicated Percentage of total 1 74.42856735775541 40.24718478251434 2 14.052208862349055 15.19744008955499 3 4.848055149515852 7.864745152241058 4 2.2069016535516663 4.77352080259141 5 1.1977436129503094 3.2383942230660456 6 0.7755567159384297 2.5162898250338137 7 0.47354140324692784 1.792469790118167 8 0.32274997217267604 1.3962141935256827 9 0.25179721074988537 1.2254321567221105 >10 1.3129321750408591 12.76654987132261 >50 0.08771496070533397 3.2317475406625857 >100 0.03945624463479071 3.8078423856330486 >500 0.001902638666594733 0.6466323809057708 >1k 7.134894999724481E-4 0.6840139399181631 >5k 1.5855322221609958E-4 0.6115228661903106 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8337 0.3572810641036025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5928 0.25404367854218013 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4092 0.17536213437830653 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3778 0.16190570471193597 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.570974309790152E-5 2 0.0 0.0 0.0 0.0 8.570974309790152E-5 3 0.0 0.0 0.0 0.0 8.570974309790152E-5 4 0.0 0.0 0.0 0.0 8.570974309790152E-5 5 0.0 0.0 0.0 0.0 8.570974309790152E-5 6 0.0 0.0 0.0 0.0 8.570974309790152E-5 7 0.0 0.0 0.0 0.0 8.570974309790152E-5 8 0.0 0.0 0.0 0.0 8.570974309790152E-5 9 0.0 0.0 0.0 0.0 8.570974309790152E-5 10-11 0.0 0.0 0.0 0.0 8.570974309790152E-5 12-13 0.0 0.0 0.0 0.0 8.570974309790152E-5 14-15 0.0 0.0 0.0 0.0 1.2856461464685228E-4 16-17 0.0 0.0 0.0 4.285487154895076E-5 2.5712922929370456E-4 18-19 0.0 0.0 0.0 8.570974309790152E-5 2.5712922929370456E-4 20-21 0.0 0.0 0.0 8.570974309790152E-5 3.214115366171307E-4 22-23 0.0 0.0 0.0 8.570974309790152E-5 4.0712127971503226E-4 24-25 0.0 0.0 0.0 2.5712922929370456E-4 4.714035870384584E-4 26-27 0.0 0.0 0.0 3.214115366171307E-4 4.714035870384584E-4 28-29 0.0 0.0 0.0 7.713876878811137E-4 4.714035870384584E-4 30-31 0.0 0.0 0.0 0.0019284692197027843 5.142584585874091E-4 32-33 0.0 0.0 0.0 0.0033426799808181596 5.571133301363599E-4 34-35 0.0 0.0 0.0 0.005913972273755206 5.571133301363599E-4 36-37 0.0 0.0 0.0 0.00951378148386707 5.571133301363599E-4 38-39 0.0 0.0 0.0 0.013670704024115294 5.571133301363599E-4 40-41 0.0 0.0 0.0 0.02022749937110476 5.571133301363599E-4 42-43 0.0 0.0 0.0 0.031048354437214827 5.571133301363599E-4 44-45 0.0 0.0 0.0 0.046926084346101085 5.571133301363599E-4 46-47 0.0 0.0 0.0 0.07401036316503797 5.999682016853107E-4 48-49 0.0 0.0 0.0 0.11345827242584713 5.999682016853107E-4 50-51 0.0 0.0 0.0 0.15530605449339757 5.999682016853107E-4 52-53 0.0 0.0 0.0 0.19760381271221197 6.213956374597861E-4 54-55 0.0 0.0 0.0 0.2482368434472973 6.856779447832122E-4 56-57 0.0 0.0 0.0 0.3043767251764228 6.856779447832122E-4 58-59 0.0 0.0 0.0 0.3727945276043227 6.856779447832122E-4 60-61 0.0 0.0 0.0 0.4562329625101298 6.856779447832122E-4 62-63 0.0 0.0 0.0 0.544813982001811 7.713876878811137E-4 64-65 0.0 0.0 0.0 0.6433373316928488 8.142425594300645E-4 66-67 0.0 0.0 0.0 0.7713234055737903 8.142425594300645E-4 68-69 0.0 0.0 0.0 0.9255366608426896 8.142425594300645E-4 70-71 0.0 0.0 0.0 1.0999988429184682 8.356699952045399E-4 72-73 0.0 0.0 0.0 1.2823891762308026 8.99952302527966E-4 74-75 0.0 0.0 0.0 1.463558145703992 8.99952302527966E-4 76-77 0.0 0.0 0.0 1.6635618312229452 8.99952302527966E-4 78-79 0.0 0.0 0.0 1.8966494775776885 8.99952302527966E-4 80-81 0.0 0.0 0.0 2.142336456167823 9.213797383024414E-4 82-83 4.285487154895076E-5 0.0 0.0 2.4291212565734015 9.428071740769168E-4 84-85 4.285487154895076E-5 0.0 0.0 2.7428403437474955 9.856620456258674E-4 86-87 4.285487154895076E-5 0.0 0.0 3.115849145709563 0.0010499443529492936 88-89 4.285487154895076E-5 0.0 0.0 3.5701964938715394 0.0011142266602727198 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4405 0.0 48.125042 1 GTATCAA 7835 0.0 43.133305 1 ATCAACG 9890 0.0 33.771095 3 TATCAAC 9935 0.0 33.666676 2 TCAACGC 9955 0.0 33.507908 4 CAACGCA 10140 0.0 32.897984 5 AACGCAG 10500 0.0 31.761177 6 ACGCAGA 12160 0.0 27.192125 7 CGCAGAG 12225 0.0 27.047544 8 GTACATG 7725 0.0 24.857933 1 GCAGAGT 13210 0.0 24.598658 9 TACATGG 7555 0.0 24.400162 2 ACATGGG 7675 0.0 22.9039 3 GAGTACT 7890 0.0 22.365776 12-13 ATAACGC 155 3.9022198E-5 21.456182 3 AGAGTAC 11785 0.0 21.141317 10-11 CAGAGTA 12520 0.0 20.791813 10-11 AGTACTT 8365 0.0 20.669863 12-13 GTCTACG 230 8.967436E-8 20.6659 1 GTACTTT 8455 0.0 20.531685 14-15 >>END_MODULE