FastQCFastQC Report
Thu 26 May 2016
SRR937163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333457
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86410.37030894505448353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69350.29719853419197356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46750.20034652449134482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49450.047.1765371
GTATCAA82150.038.7505721
ATCAACG104850.029.988923
TCAACGC105850.029.6607324
TATCAAC106200.029.5182552
CAACGCA108950.028.860385
AACGCAG112300.028.0417466
ACGCAGA130550.023.9069927
CGCAGAG131600.023.6440478
GTACATG76750.022.2242411
TACATGG75400.021.6699712
GCAGAGT142800.021.0910159
TATACCG2056.838818E-720.8525875
GAGTACT89250.020.57205812-13
ACATGGG76800.020.4091073
AGAGTAC129050.019.60189410-11
GTACTTT93900.019.27217114-15
AGTACTT94000.019.07767912-13
CAGAGTA137350.019.00533110-11
TGGTATC24300.018.1780782
Produced by FastQC (version 0.11.5)