##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937163_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2333457 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.063145367581235 31.0 31.0 34.0 30.0 34.0 2 32.296139590316 34.0 31.0 34.0 31.0 34.0 3 32.360165196958846 34.0 31.0 34.0 30.0 34.0 4 36.02982827624422 37.0 35.0 37.0 35.0 37.0 5 35.94472835796846 37.0 35.0 37.0 35.0 37.0 6 35.98024947534923 37.0 35.0 37.0 35.0 37.0 7 35.915368914018984 37.0 35.0 37.0 35.0 37.0 8 35.87705023062349 37.0 35.0 37.0 35.0 37.0 9 37.55566526402672 39.0 37.0 39.0 35.0 39.0 10-11 37.46166096054052 39.0 37.0 39.0 34.5 39.0 12-13 37.34218372140562 39.0 37.0 39.0 34.0 39.0 14-15 38.528555272284855 40.0 38.0 41.0 34.0 41.0 16-17 38.46518470235363 40.0 38.0 41.0 34.0 41.0 18-19 38.430403902878865 40.0 38.0 41.0 34.0 41.0 20-21 38.296802555178864 40.0 38.0 41.0 34.0 41.0 22-23 38.23270088113902 40.0 38.0 41.0 33.5 41.0 24-25 38.27181859361454 40.0 38.0 41.0 34.0 41.0 26-27 38.094401353871106 40.0 37.5 41.0 33.0 41.0 28-29 37.938647037421305 40.0 37.0 41.0 33.0 41.0 30-31 38.00103880208634 40.0 37.0 41.0 33.0 41.0 32-33 37.90375224398821 40.0 37.0 41.0 33.0 41.0 34-35 37.76212782151117 40.0 37.0 41.0 33.0 41.0 36-37 37.57786151619678 40.0 37.0 41.0 33.0 41.0 38-39 37.44226484567747 40.0 37.0 41.0 32.0 41.0 40-41 37.23706779254985 39.5 36.0 41.0 32.0 41.0 42-43 37.0321919366845 39.0 36.0 41.0 31.0 41.0 44-45 36.72881394428953 39.0 36.0 40.5 31.0 41.0 46-47 36.393307654694304 39.0 35.0 40.0 30.0 41.0 48-49 36.069211903197704 39.0 35.0 40.0 29.5 41.0 50-51 35.808629856903295 39.0 35.0 40.0 29.0 41.0 52-53 35.53533962699977 38.0 35.0 40.0 28.0 41.0 54-55 35.287505405070675 38.0 34.5 40.0 27.5 41.0 56-57 34.93493130578365 38.0 34.0 40.0 27.0 41.0 58-59 34.63396197144408 38.0 34.0 40.0 26.0 41.0 60-61 34.24195624774744 37.0 33.0 40.0 26.0 41.0 62-63 33.822268634048115 36.5 33.0 39.0 25.0 41.0 64-65 33.35744648390778 36.0 32.0 39.0 23.5 40.0 66-67 32.87615949211835 35.5 32.0 39.0 22.5 40.0 68-69 32.368610606495 35.0 31.0 38.0 21.5 40.0 70-71 31.89215507292399 35.0 31.0 37.5 20.5 39.5 72-73 31.372940662716303 34.5 30.0 37.0 18.5 39.0 74-75 30.85961086919536 34.0 30.0 36.5 18.0 39.0 76-77 29.629146583802488 32.5 28.5 35.0 17.5 37.5 78-79 30.286978718699338 34.0 30.0 35.0 17.5 37.5 80-81 30.095167813248757 34.0 30.0 35.0 14.0 37.0 82-83 29.646663083999407 34.0 29.0 35.0 8.5 37.0 84-85 29.155155419619902 34.0 29.0 35.0 6.5 36.0 86-87 28.695099159744533 33.0 29.0 35.0 2.0 36.0 88-89 28.147972728873942 33.0 27.5 35.0 2.0 35.0 90-91 27.478881119300677 32.5 26.0 34.5 2.0 35.0 92-93 26.847184027817953 32.0 25.0 34.0 2.0 35.0 94-95 26.209008779677536 32.0 24.5 34.0 2.0 35.0 96-97 25.364676315012446 32.0 21.5 34.0 2.0 35.0 98-99 24.16821522745009 31.0 10.0 34.0 2.0 35.0 100-101 21.135916796409788 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14853169240662112 1101 2 0.1676145511415612 1101 3 0.110177063402638 1101 4 0.06964883625687435 1101 5 0.08502202952391968 1101 6 0.10610218792864146 1101 7 0.12327836320681484 1101 8 0.007652396464187916 1101 9 0.16739264787536 1101 10-11 0.12036922415254736 1101 12-13 0.17212673786706034 1101 14-15 0.16736193984062453 1101 16-17 0.17436282792556312 1101 18-19 0.1608440204223598 1101 20-21 0.22113883351195085 1101 22-23 0.24656812709367415 1101 24-25 0.13286261649066233 1101 26-27 0.16581752238734282 1101 28-29 0.033433629795915465 1101 30-31 0.21478812588762963 1101 32-33 0.20082923054074797 1101 34-35 0.20190916020197847 1101 36-37 0.3060785510117583 1101 38-39 0.31384721334892873 1101 40-41 0.27675809667206863 1101 42-43 0.4488708730220523 1101 44-45 0.38589789395783924 1101 46-47 0.535598710594634 1101 48-49 0.5189734446190855 1101 50-51 0.4513773826171601 1101 52-53 0.4245933156253656 1101 54-55 0.4219961143309021 1101 56-57 0.5039102334419283 1101 58-59 0.48738008414126455 1101 60-61 0.5696685241192725 1101 62-63 0.637807909550574 1101 64-65 0.6964802178079665 1101 66-67 0.6006757106679856 1101 68-69 0.5621612964096201 1101 70-71 0.4761318313256737 1101 72-73 0.4817248493518278 1101 74-75 0.4319767268843968 1101 76-77 0.5639155023151794 1101 78-79 0.7399596904217809 1101 80-81 0.6580419238634612 1101 82-83 0.4682148268883104 1101 84-85 0.7153738847712354 1101 86-87 0.8796723605589918 1101 88-89 1.0997621201233265 1101 90-91 1.263841972167775 1101 92-93 1.3416795653250908 1101 94-95 1.4720926682995774 1101 96-97 1.6691471196023393 1101 98-99 2.005394572761272 1101 100-101 2.5259903542420155 1102 1 0.19644066563325424 1102 2 0.2231281623750192 1102 3 0.1924192315561939 1102 4 0.08661097566810838 1102 5 0.04546530561162854 1102 6 0.08275536325280086 1102 7 0.04802804982265485 1102 8 0.08661387956114197 1102 9 0.15767035233537996 1102 10-11 0.022864548011739316 1102 12-13 0.08788091034770673 1102 14-15 0.14611775350094547 1102 16-17 0.16243989994443098 1102 18-19 0.18128849607609254 1102 20-21 0.15909512164102324 1102 22-23 0.2112470629505694 1102 24-25 0.0020654526665779827 1102 26-27 0.08693945738780684 1102 28-29 -0.029831512115300995 1102 30-31 0.09768339766714718 1102 32-33 0.16130530264550202 1102 34-35 0.13190192538092305 1102 36-37 0.19011478933026638 1102 38-39 0.12918662947345894 1102 40-41 0.20613093833959084 1102 42-43 0.143320230850442 1102 44-45 0.06662408838287348 1102 46-47 0.17118428988063528 1102 48-49 0.29610151217926983 1102 50-51 0.2577928843194073 1102 52-53 0.25685759400662533 1102 54-55 0.3463982911985468 1102 56-57 0.30999216124104834 1102 58-59 0.24552467459091076 1102 60-61 0.47913669484608334 1102 62-63 0.3835119267770466 1102 64-65 0.4185900121759616 1102 66-67 0.4445988746228622 1102 68-69 0.4476740941442685 1102 70-71 0.507926683473741 1102 72-73 0.5713045107787487 1102 74-75 0.489831166145386 1102 76-77 0.3463503692283503 1102 78-79 0.3750537726414933 1102 80-81 0.5572799532187567 1102 82-83 0.6608982226095854 1102 84-85 0.6806520619666365 1102 86-87 0.8077334600311836 1102 88-89 0.8580384363171127 1102 90-91 1.1040672620924425 1102 92-93 1.0461694286966896 1102 94-95 0.9910630467006136 1102 96-97 1.2438968751587502 1102 98-99 1.6280905294555588 1102 100-101 2.3095530397414095 1103 1 0.17294079904507242 1103 2 0.12214151454922728 1103 3 0.13569512618983737 1103 4 0.0536365717491023 1103 5 0.04668779754445751 1103 6 -0.021823709979798878 1103 7 0.07863301993740635 1103 8 0.03821327236249772 1103 9 0.008299086741935469 1103 10-11 0.1466545228383822 1103 12-13 0.1397725599673123 1103 14-15 0.22425700345672794 1103 16-17 0.22478743822162528 1103 18-19 0.08678186495926354 1103 20-21 0.11851099774691676 1103 22-23 0.14760093304244037 1103 24-25 0.14531191675381905 1103 26-27 0.14598587770397842 1103 28-29 0.1847024309483274 1103 30-31 0.08446948206535154 1103 32-33 0.14222536448462364 1103 34-35 0.1962862173639195 1103 36-37 0.147914283864381 1103 38-39 0.34408538139843614 1103 40-41 0.4125571406198887 1103 42-43 0.4038440745897276 1103 44-45 0.46907976518050987 1103 46-47 0.4053083338875467 1103 48-49 0.3401356556104673 1103 50-51 0.42045426514667383 1103 52-53 0.4676185809942197 1103 54-55 0.4328825750868859 1103 56-57 0.41145594368085625 1103 58-59 0.4250301714574789 1103 60-61 0.44285872458442554 1103 62-63 0.42866103321769344 1103 64-65 0.4362883635656871 1103 66-67 0.4009491271059673 1103 68-69 0.39179338129546437 1103 70-71 0.37827218509716687 1103 72-73 0.22427369814589682 1103 74-75 0.4133902435951917 1103 76-77 0.35017084389459185 1103 78-79 0.36597207564320655 1103 80-81 0.5156907436862532 1103 82-83 0.5371117454709733 1103 84-85 0.5694399231903695 1103 86-87 0.6595594530497983 1103 88-89 0.7998818278353212 1103 90-91 1.0414745358844648 1103 92-93 1.149854093133495 1103 94-95 1.0098939494738808 1103 96-97 1.1923503973805616 1103 98-99 1.4752878820055244 1103 100-101 2.1908877275127914 1104 1 0.12482309285140047 1104 2 0.1292794954891292 1104 3 0.0973071136449235 1104 4 0.0571241476896418 1104 5 0.0477870453955731 1104 6 0.04194500398416068 1104 7 0.10664831575532929 1104 8 0.11244026416137842 1104 9 0.07724050570110563 1104 10-11 0.10925632892615766 1104 12-13 0.1467295579253758 1104 14-15 0.14953080405888386 1104 16-17 0.11564923882784228 1104 18-19 0.08336113995528649 1104 20-21 0.10537003531592148 1104 22-23 0.06329229410120973 1104 24-25 -0.08128449069101151 1104 26-27 -0.03611582067389918 1104 28-29 0.10285579771724684 1104 30-31 0.15117584463611422 1104 32-33 0.0522075443106047 1104 34-35 -0.005422526961083918 1104 36-37 0.18650355717144151 1104 38-39 0.26760922473058457 1104 40-41 0.17113418657712742 1104 42-43 0.1845372510408012 1104 44-45 0.2258142638035423 1104 46-47 0.133543499502899 1104 48-49 0.3027110146181968 1104 50-51 0.3654192568990311 1104 52-53 0.37871937622707463 1104 54-55 0.3844792100878536 1104 56-57 0.3450783744456416 1104 58-59 0.32064708626147365 1104 60-61 0.6382679059183474 1104 62-63 0.5857605824542702 1104 64-65 0.5496443412631677 1104 66-67 0.5649293165742577 1104 68-69 0.6659277830301491 1104 70-71 0.5846649955368406 1104 72-73 0.6914948938370991 1104 74-75 0.643370764789772 1104 76-77 0.7064251484982087 1104 78-79 0.6517652908293954 1104 80-81 0.7955750844864617 1104 82-83 0.7142819989280582 1104 84-85 0.6739969624119446 1104 86-87 0.7346642552934028 1104 88-89 0.9478144044686445 1104 90-91 1.0162665748582569 1104 92-93 1.2587898687004362 1104 94-95 1.3189546786446549 1104 96-97 1.7093335187832785 1104 98-99 1.8352321863765262 1104 100-101 2.303521694868092 1105 1 -0.012859999363953989 1105 2 0.030536704061816522 1105 3 0.04298139109219079 1105 4 -0.012774531687227864 1105 5 -0.012785744983446534 1105 6 -0.04089266405562597 1105 7 -0.04879126580370752 1105 8 -0.05676463887762395 1105 9 -0.08444448957123285 1105 10-11 -0.02664238890883297 1105 12-13 0.043391485558345266 1105 14-15 0.05934995988646108 1105 16-17 0.09938703380012726 1105 18-19 0.086092804164565 1105 20-21 0.09750079956368296 1105 22-23 0.019546329878430413 1105 24-25 0.07897739881465071 1105 26-27 -9.697966135888691E-4 1105 28-29 0.06109197775834474 1105 30-31 -0.04964758314500273 1105 32-33 0.012255184832156374 1105 34-35 -0.024394892729297624 1105 36-37 0.0015378004116541888 1105 38-39 -0.0011814470646598352 1105 40-41 -0.029124568384439442 1105 42-43 -0.21198919440066533 1105 44-45 -0.005825650687086181 1105 46-47 0.12959657916147194 1105 48-49 0.11075843969152999 1105 50-51 0.1582825967723167 1105 52-53 0.17394210150640532 1105 54-55 0.19877959576497517 1105 56-57 0.20528799398104525 1105 58-59 0.15818564001673963 1105 60-61 0.3367838460882524 1105 62-63 0.39064927202254296 1105 64-65 0.35199339880127667 1105 66-67 0.34681038006139175 1105 68-69 0.3322271849235747 1105 70-71 0.28103854924763993 1105 72-73 0.2626955152046371 1105 74-75 0.39376620117475625 1105 76-77 0.3110517231879193 1105 78-79 0.32176711448895645 1105 80-81 0.2979210302245292 1105 82-83 0.33397320084583626 1105 84-85 0.5151413273969361 1105 86-87 0.6188050128647404 1105 88-89 0.8869480607261835 1105 90-91 0.8721460884415251 1105 92-93 0.8587246552960828 1105 94-95 1.0363534040265456 1105 96-97 1.3952808882163872 1105 98-99 1.3702195706635685 1105 100-101 1.8360866306916144 1106 1 -0.12324458145456418 1106 2 0.010983601289616729 1106 3 -0.05606415190840153 1106 4 0.012460456975084355 1106 5 -0.007242024894544841 1106 6 0.008050969019173237 1106 7 -0.09619720007545851 1106 8 -0.13571622013968465 1106 9 -0.26061701582442254 1106 10-11 -0.34753858918066527 1106 12-13 -0.47268942856187834 1106 14-15 -0.21127609195550434 1106 16-17 -0.2567105299174699 1106 18-19 -0.131719342059597 1106 20-21 -0.015499714201745007 1106 22-23 0.027991138318128606 1106 24-25 0.00901969171491146 1106 26-27 -0.030667518170716335 1106 28-29 0.00946996296594449 1106 30-31 -0.09103903305399541 1106 32-33 0.011320457774260717 1106 34-35 -0.4124854278660166 1106 36-37 0.01600389470390695 1106 38-39 0.09722610494987549 1106 40-41 -0.048420957074405635 1106 42-43 -0.093107584167619 1106 44-45 -0.05553847042737203 1106 46-47 -0.10532552972757259 1106 48-49 0.022995163014790876 1106 50-51 0.2099314802599821 1106 52-53 0.05709964537039269 1106 54-55 0.011693207850719034 1106 56-57 -0.007079081861746772 1106 58-59 0.13438250548551878 1106 60-61 0.1521060287256759 1106 62-63 0.059441113832249926 1106 64-65 0.01257662936279047 1106 66-67 0.013329456272927587 1106 68-69 0.21598022451498622 1106 70-71 0.18226826984693645 1106 72-73 0.24394673076015394 1106 74-75 0.24329819122321794 1106 76-77 -0.03822197887984302 1106 78-79 0.042799513188917615 1106 80-81 0.20440470496267338 1106 82-83 0.239661607172458 1106 84-85 0.33007818596304617 1106 86-87 0.5115374242683117 1106 88-89 0.5094458713209917 1106 90-91 0.5718287230576244 1106 92-93 0.751025368834199 1106 94-95 0.48896523232321343 1106 96-97 0.9059930098202784 1106 98-99 1.2960972720073407 1106 100-101 1.6007707099216617 1107 1 -0.017186546497832467 1107 2 0.002251085714235046 1107 3 0.003891364321503943 1107 4 -0.038677697251479515 1107 5 -0.06448306147547811 1107 6 -0.017405989600007388 1107 7 -0.0104526120654711 1107 8 -0.01383978078064274 1107 9 -0.0659471896383863 1107 10-11 -0.07151134135637705 1107 12-13 -0.04905545605213035 1107 14-15 -0.06338361662157865 1107 16-17 -0.07761547767631072 1107 18-19 -0.010379859380392986 1107 20-21 -0.06859357859291748 1107 22-23 -0.04665614295989684 1107 24-25 -0.127159734238397 1107 26-27 -0.0597913575927862 1107 28-29 -0.0858837860340742 1107 30-31 -0.007169248079087254 1107 32-33 0.02344470930755449 1107 34-35 -0.04386193995057397 1107 36-37 0.056000500846792534 1107 38-39 -0.046977368905302797 1107 40-41 -0.042354437474877216 1107 42-43 -0.0169648845967032 1107 44-45 -0.09966770365808486 1107 46-47 -0.016152539981867164 1107 48-49 0.017158334816691934 1107 50-51 -0.023364076211372264 1107 52-53 0.03551501711896066 1107 54-55 -0.0012504177583636533 1107 56-57 0.10542334232190598 1107 58-59 0.09473498178911655 1107 60-61 0.282831950521512 1107 62-63 0.17398682181509173 1107 64-65 0.2739942313603123 1107 66-67 0.28135962523852953 1107 68-69 0.3284462859438051 1107 70-71 0.17829018446718692 1107 72-73 0.15666187705963708 1107 74-75 0.20086043213859028 1107 76-77 0.20137736416638674 1107 78-79 0.31202571376587684 1107 80-81 0.3058152768275981 1107 82-83 0.4262637501508131 1107 84-85 0.47244806790530447 1107 86-87 0.5857704731640929 1107 88-89 0.8131951743681896 1107 90-91 0.9327842150456931 1107 92-93 1.1289804487110509 1107 94-95 1.0654473176059973 1107 96-97 1.1985134941721718 1107 98-99 1.3896750034562722 1107 100-101 2.0431387737261737 1108 1 -0.04452273755723368 1108 2 0.014279867098196064 1108 3 -0.04598677407408758 1108 4 -0.03282099180139397 1108 5 -0.024322620569165565 1108 6 -0.07876606308439449 1108 7 -0.08150588824428695 1108 8 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88-89 1.150153552544225 2315 90-91 1.4112515305667301 2315 92-93 1.6959719324974891 2315 94-95 2.0256661889737373 2315 96-97 2.004057026685082 2315 98-99 2.3565970785424497 2315 100-101 3.1208363545600157 2316 1 -0.14369247472254898 2316 2 -0.09812887768195822 2316 3 -0.2800049514260863 2316 4 -0.08931030646608917 2316 5 -0.09973013714986223 2316 6 -0.1787631154056939 2316 7 -0.4611767307891128 2316 8 -0.16203943065730186 2316 9 -0.28074984739282627 2316 10-11 -0.16108721467538345 2316 12-13 0.044926799060476696 2316 14-15 0.04973307789580872 2316 16-17 -0.037371187073944157 2316 18-19 0.013223625938294958 2316 20-21 0.14723159845808453 2316 22-23 0.17298856142459584 2316 24-25 -0.011651856463267052 2316 26-27 0.1515985922759171 2316 28-29 0.13136675108695783 2316 30-31 0.05801237840297091 2316 32-33 -0.04188412812381159 2316 34-35 -0.027448913770896866 2316 36-37 0.25350985073140464 2316 38-39 0.16039014124031326 2316 40-41 0.23567134433462655 2316 42-43 0.0975562819506024 2316 44-45 0.2559680884959761 2316 46-47 0.3722078483367497 2316 48-49 0.3087454279972448 2316 50-51 0.020232247493559896 2316 52-53 0.3242583528395002 2316 54-55 0.3713196069094167 2316 56-57 0.3096337744059312 2316 58-59 -0.07048344743026291 2316 60-61 0.3312623635079106 2316 62-63 0.5731015667820714 2316 64-65 0.668647887761999 2316 66-67 0.7069056982869952 2316 68-69 0.6560115362024206 2316 70-71 0.7547762193203766 2316 72-73 0.6637946454212162 2316 74-75 0.723968572506223 2316 76-77 0.785394034811727 2316 78-79 0.7370953405662242 2316 80-81 0.8099182847053719 2316 82-83 1.121741986802359 2316 84-85 0.9955721229701595 2316 86-87 0.9672984830312075 2316 88-89 1.32216211158471 2316 90-91 1.4478733373501456 2316 92-93 1.44603105744822 2316 94-95 1.782140840324228 2316 96-97 1.9171883966946517 2316 98-99 2.278178624049893 2316 100-101 2.6736452589652906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 18.0 5 50.0 6 103.0 7 238.0 8 608.0 9 1167.0 10 1940.0 11 3217.0 12 4972.0 13 6981.0 14 9293.0 15 12514.0 16 16249.0 17 21662.0 18 19832.0 19 14401.0 20 13583.0 21 13955.0 22 15510.0 23 17125.0 24 20244.0 25 24080.0 26 29445.0 27 36456.0 28 44790.0 29 55867.0 30 69665.0 31 87069.0 32 110213.0 33 142727.0 34 192501.0 35 265991.0 36 372132.0 37 452950.0 38 243000.0 39 12898.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.828885689714156 20.207061296969062 15.514849439196482 22.449203574120297 2 15.956197178692385 20.934690461405545 39.19215138740504 23.91696097249703 3 19.388315276433207 24.460489308352372 30.069849155137636 26.08134626007679 4 13.440316234668135 17.33170999079906 36.87117439918541 32.35679937534739 5 13.629374339493912 37.689709347323564 34.41882749830936 14.262088814873161 6 32.23119174683742 37.007281471224886 16.724542170693525 14.036984611244174 7 28.539501692124603 32.80767547891391 20.522640871462382 18.130181957499108 8 26.433741868823805 35.651053351315234 19.32321872655035 18.591986053310603 9 26.03257741625408 16.75578337205271 20.181987497519774 37.02965171417343 10-11 24.845540329219695 27.14313998500937 27.1814736676099 20.82984601816104 12-13 25.664162452559825 24.137591133029204 27.919883720008304 22.278362694402666 14-15 22.559725763105984 25.72700075467429 26.07594654626162 25.63732693595811 16-17 21.963314515759237 29.01189094120869 26.824899708886857 22.19989483414522 18-19 21.958150503737585 27.468387032630126 29.304075455429434 21.26938700820285 20-21 22.463876495476875 26.54405295499845 29.730103841817257 21.26196670770742 22-23 22.313737027476368 26.31518724596267 29.5821429420879 21.78893278447306 24-25 22.253832458415232 26.544848811194893 29.558468306565818 21.642850423824058 26-27 21.899930016495194 26.968205718076614 29.422256811606378 21.709607453821814 28-29 21.930759698487144 27.13902852380884 29.411881931106837 21.51832984659718 30-31 22.036143470795714 26.82641007383701 29.3673681957256 21.770078259641675 32-33 22.018001655915782 26.79088749200755 29.201822021622903 21.989288830453766 34-35 22.315881543992454 26.930365547768826 29.16430429187253 21.589448616366187 36-37 22.48692497308713 26.680511653101664 29.110319628910936 21.72224374490027 38-39 22.164575240453484 26.94180368984967 29.117761191503334 21.775859878193522 40-41 22.380313843366302 26.88093245343711 29.025261661131964 21.713492042064626 42-43 22.152650928444263 26.837430751335685 28.938162860686646 22.071755459533406 44-45 22.256613256640257 27.075986401292162 28.916303150218752 21.75109719184883 46-47 22.59227403804741 27.26673343455654 28.32424167233422 21.816750855061827 48-49 22.493664978613275 27.349914740233057 28.164093017355796 21.992327263797876 50-51 22.561182828738648 27.558232270832505 28.050527607751075 21.830057292677772 52-53 22.75758670504749 27.440638503302182 27.923827180016602 21.877947611633726 54-55 22.55098336931 27.57680985764897 27.928026957428397 21.944179815612628 56-57 22.567289647934373 27.764107073753664 27.818747034978575 21.84985624333339 58-59 22.504614398294034 27.456816217311914 27.89241455917122 22.146154825222837 60-61 22.383249402067403 27.55281112958156 28.106646062044426 21.957293406306608 62-63 22.372192845207774 27.553753936755637 28.246974338931462 21.827078879105123 64-65 22.28924724132478 27.56395339618429 28.08751136189782 22.05928800059311 66-67 22.272662406035337 27.578738326868674 27.893485930959944 22.25511333613604 68-69 22.40285550580105 27.433031763602244 28.072105035575973 22.092007695020737 70-71 22.386613509483997 27.39321958793327 27.91300632495049 22.307160577632242 72-73 22.43411373616696 27.44462819692533 27.768890138348617 22.35236792855909 74-75 22.361286280398566 27.699631919508267 27.552296871122973 22.386784928970194 76-77 22.459702492910733 27.491014404807974 27.81489009653917 22.234393005742124 78-79 22.412133585491397 27.516791610044667 27.76342139580888 22.307653408655057 80-81 22.41631193546742 27.61117946463123 27.648484630314595 22.324023969586754 82-83 22.392592421172445 27.60657190560921 27.639613139011455 22.361222534206888 84-85 22.403727004864674 27.639845713792273 27.5678494833092 22.38857779803386 86-87 22.41211215805562 27.596523098561494 27.555853825461536 22.43551091792135 88-89 22.364243266535443 27.469029855703365 27.569203117949037 22.59752375981216 90-91 22.38527737674734 27.445332652158992 27.51937178811049 22.65001818298317 92-93 22.21527489248126 27.392395016693232 27.714067335624527 22.67826275520098 94-95 22.185795581405614 27.396712259964506 27.598708697010487 22.81878346161939 96-97 22.266384167353415 27.35966422351044 27.499778226039734 22.874173383096412 98-99 21.99678844904459 27.448518332496235 27.44943971420882 23.105253504250356 100-101 22.27351658579268 27.153386410212942 27.636144424945346 22.936952579049027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 279.0 1 208.5 2 195.0 3 290.5 4 294.0 5 296.5 6 510.0 7 1106.5 8 1835.0 9 2217.0 10 2207.5 11 2122.5 12 2222.0 13 2403.5 14 2553.0 15 2757.0 16 2963.0 17 3127.0 18 3417.5 19 3715.0 20 4122.0 21 4808.0 22 5699.5 23 7036.5 24 8860.0 25 11395.5 26 14770.5 27 18926.5 28 22755.0 29 26309.0 30 30164.0 31 34616.5 32 40475.5 33 44258.5 34 47382.0 35 51914.0 36 57415.5 37 62150.5 38 64902.0 39 67423.5 40 69726.0 41 72970.5 42 75130.0 43 77550.5 44 81368.5 45 84070.0 46 85033.0 47 86384.5 48 87412.5 49 86052.5 50 83692.0 51 80741.5 52 79345.0 53 78989.5 54 77642.5 55 71935.5 56 64484.5 57 59265.5 58 54659.5 59 48081.0 60 38963.5 61 31743.5 62 26189.5 63 19501.0 64 13488.0 65 9781.5 66 7331.5 67 5732.0 68 4446.5 69 3466.0 70 2556.0 71 1673.0 72 1189.0 73 927.5 74 669.0 75 440.5 76 297.5 77 197.5 78 133.5 79 89.0 80 62.5 81 37.5 82 18.5 83 12.0 84 4.5 85 1.5 86 1.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039040787981094144 2 0.0 3 0.0 4 0.0 5 8.142425594300645E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005292576636295419 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.7141948619580307E-4 22-23 6.642505090087368E-4 24-25 2.1427435774475383E-5 26-27 0.002142743577447538 28-29 0.0038783658751800443 30-31 2.7855666506817995E-4 32-33 2.1427435774475383E-4 34-35 0.0 36-37 4.2854871548950767E-5 38-39 6.428230732342614E-5 40-41 0.0 42-43 0.008528119438241203 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.7141948619580307E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.856938439405568E-4 84-85 6.428230732342614E-5 86-87 0.0 88-89 0.0 90-91 0.009920902763582103 92-93 0.0011356540960471952 94-95 0.0 96-97 0.0 98-99 2.1427435774475383E-4 100-101 0.012877888900459704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2333457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.002826659579725 #Duplication Level Percentage of deduplicated Percentage of total 1 73.57988738751384 38.999420168317926 2 14.393859755874121 15.258305072057931 3 5.019613161104238 7.981610588284589 4 2.3712759235453373 5.027373069508333 5 1.257555874262766 3.332700800914281 6 0.7499488032258632 2.3849643852563815 7 0.5018814910257609 1.8620796370742876 8 0.35570409642133144 1.508265805177781 9 0.2523798106063214 1.2039159018549974 >10 1.382329905919275 13.177188979845846 >50 0.0924626798116193 3.3265454924704776 >100 0.03978929846878862 3.7858656785639697 >500 0.0025040531426719507 0.8674018019230731 >1k 6.462072626246292E-4 0.617499318318388 >5k 1.615518156561573E-4 0.6668633004317556 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8641 0.37030894505448353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6935 0.29719853419197356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4675 0.20034652449134482 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 4.285487154895076E-5 16-17 0.0 0.0 0.0 0.0 8.570974309790152E-5 18-19 0.0 0.0 0.0 4.285487154895076E-5 8.570974309790152E-5 20-21 0.0 0.0 0.0 6.428230732342614E-5 1.2856461464685228E-4 22-23 0.0 0.0 0.0 8.570974309790152E-5 1.9284692197027843E-4 24-25 0.0 0.0 0.0 2.9998410084265535E-4 2.1427435774475383E-4 26-27 0.0 0.0 0.0 3.6426640816608147E-4 2.1427435774475383E-4 28-29 0.0 0.0 0.0 7.713876878811137E-4 2.1427435774475383E-4 30-31 0.0 0.0 0.0 0.0019284692197027843 2.1427435774475383E-4 32-33 0.0 0.0 0.0 0.003321252545043684 2.1427435774475383E-4 34-35 0.0 0.0 0.0 0.005978254581078632 2.1427435774475383E-4 36-37 0.0 0.0 0.0 0.009599491226964971 2.1427435774475383E-4 38-39 0.0 0.0 0.0 0.013799268638762146 2.1427435774475383E-4 40-41 0.0 0.0 0.0 0.020441773728849515 2.1427435774475383E-4 42-43 0.0 0.0 0.0 0.031155491616087203 2.357017935192292E-4 44-45 0.0 0.0 0.0 0.046926084346101085 2.5712922929370456E-4 46-47 0.0 0.0 0.0 0.07390322598616558 2.5712922929370456E-4 48-49 0.0 0.0 0.0 0.11309400601768106 2.9998410084265535E-4 50-51 0.0 0.0 0.0 0.15489893321368253 2.9998410084265535E-4 52-53 0.0 0.0 0.0 0.19728240117559484 2.9998410084265535E-4 54-55 0.0 0.0 0.0 0.24787257703913124 2.9998410084265535E-4 56-57 0.0 0.0 0.0 0.30439815261219727 3.214115366171307E-4 58-59 0.0 0.0 0.0 0.37311593914093977 3.428389723916061E-4 60-61 0.0 0.0 0.0 0.45691864045491304 3.8569384394055686E-4 62-63 0.0 0.0 0.0 0.5451996758457516 3.8569384394055686E-4 64-65 0.0 0.0 0.0 0.643615888357917 3.8569384394055686E-4 66-67 0.0 0.0 0.0 0.7720733658258969 3.8569384394055686E-4 68-69 0.0 0.0 0.0 0.9264580405809921 3.8569384394055686E-4 70-71 0.0 0.0 0.0 1.101520190858456 4.2854871548950765E-4 72-73 0.0 0.0 0.0 1.2845962021155737 4.2854871548950765E-4 74-75 0.0 0.0 0.0 1.466172292868478 4.4997615126398305E-4 76-77 0.0 0.0 0.0 1.6663688253094016 4.714035870384584E-4 78-79 0.0 0.0 0.0 1.9014492231911708 4.714035870384584E-4 80-81 0.0 0.0 0.0 2.1493432276660767 4.928310228129337E-4 82-83 0.0 0.0 0.0 2.4387207478003665 5.142584585874091E-4 84-85 0.0 0.0 0.0 2.7547754254738783 5.142584585874091E-4 86-87 0.0 0.0 0.0 3.1297984063987467 5.571133301363599E-4 88-89 0.0 0.0 0.0 3.589309766582371 5.999682016853107E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4945 0.0 47.176537 1 GTATCAA 8215 0.0 38.750572 1 ATCAACG 10485 0.0 29.98892 3 TCAACGC 10585 0.0 29.660732 4 TATCAAC 10620 0.0 29.518255 2 CAACGCA 10895 0.0 28.86038 5 AACGCAG 11230 0.0 28.041746 6 ACGCAGA 13055 0.0 23.906992 7 CGCAGAG 13160 0.0 23.644047 8 GTACATG 7675 0.0 22.224241 1 TACATGG 7540 0.0 21.669971 2 GCAGAGT 14280 0.0 21.091015 9 TATACCG 205 6.838818E-7 20.852587 5 GAGTACT 8925 0.0 20.572058 12-13 ACATGGG 7680 0.0 20.409107 3 AGAGTAC 12905 0.0 19.601894 10-11 GTACTTT 9390 0.0 19.272171 14-15 AGTACTT 9400 0.0 19.077679 12-13 CAGAGTA 13735 0.0 19.005331 10-11 TGGTATC 2430 0.0 18.178078 2 >>END_MODULE