FastQCFastQC Report
Thu 26 May 2016
SRR937162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276122
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82780.36368876536494965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58850.2585538033550047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.185666673403271No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31920.14023852851472812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41800.047.78661
GTATCAA75350.043.235491
ATCAACG95150.033.7745173
TCAACGC96300.033.3726544
TATCAAC97600.033.1215252
CAACGCA97700.032.7971155
AACGCAG100800.031.8561256
CGCAGAG114400.027.5298528
ACGCAGA115850.027.4717037
TACATGG73250.024.5970382
GCAGAGT126900.024.1432259
GTACATG75450.024.1419661
GTATAGG7150.023.945811
ACATGGG74300.023.0977463
GAGTACT75650.021.91335912-13
AGAGTAC113250.021.47432110-11
GTACTTT80850.020.267414-15
AGTACTT81650.020.21580712-13
CAGAGTA120650.020.17688810-11
TCCGTTG2702.300294E-819.3528658
Produced by FastQC (version 0.11.5)