##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937162_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276122 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.874106484626044 33.0 31.0 34.0 30.0 34.0 2 32.004852551840365 33.0 31.0 34.0 30.0 34.0 3 32.04800753210944 34.0 31.0 34.0 30.0 34.0 4 35.6543063157423 37.0 35.0 37.0 35.0 37.0 5 35.657097466656005 37.0 35.0 37.0 35.0 37.0 6 35.69471847291138 37.0 35.0 37.0 35.0 37.0 7 35.64176612677176 37.0 35.0 37.0 33.0 37.0 8 35.63274947476453 37.0 35.0 37.0 33.0 37.0 9 37.28243037939091 39.0 37.0 39.0 34.0 39.0 10-11 37.17596266808194 39.0 37.0 39.0 34.0 39.0 12-13 37.05237504843765 39.0 37.0 39.0 33.0 39.0 14-15 38.27572028212899 40.0 38.0 41.0 34.0 41.0 16-17 38.235000144983445 40.0 38.0 41.0 33.0 41.0 18-19 38.19821433121774 40.0 38.0 41.0 33.5 41.0 20-21 38.12767703137178 40.0 38.0 41.0 34.0 41.0 22-23 37.98477058786831 40.0 38.0 41.0 33.0 41.0 24-25 37.834898568705896 40.0 38.0 41.0 33.0 41.0 26-27 37.668925918733706 40.0 37.0 41.0 33.0 41.0 28-29 37.72226664475806 40.0 38.0 41.0 33.0 41.0 30-31 37.57095401740328 40.0 37.0 41.0 33.0 41.0 32-33 37.378342417497834 40.0 37.0 41.0 32.0 41.0 34-35 37.26848033629129 40.0 37.0 41.0 32.0 41.0 36-37 37.290252236040075 40.0 37.0 41.0 32.0 41.0 38-39 37.21033912066225 40.0 37.0 41.0 31.5 41.0 40-41 37.04735378859306 40.0 37.0 41.0 31.0 41.0 42-43 36.82428643983056 40.0 36.0 41.0 31.0 41.0 44-45 36.62251825692999 39.5 36.0 41.0 30.5 41.0 46-47 36.316722697640984 39.0 36.0 41.0 30.0 41.0 48-49 36.020431022590174 39.0 35.5 41.0 29.0 41.0 50-51 35.1497077045958 38.5 34.0 40.0 27.0 40.5 52-53 35.23700904433066 38.5 35.0 40.0 28.0 40.5 54-55 35.55828575972642 39.0 35.0 40.0 28.0 41.0 56-57 35.358795134882925 39.0 35.0 40.0 27.5 41.0 58-59 35.116687506205736 38.0 35.0 40.0 26.5 41.0 60-61 34.82917963096881 38.0 34.0 40.0 26.0 41.0 62-63 34.50218859094548 38.0 34.0 40.0 26.0 41.0 64-65 34.13967485046935 37.0 34.0 40.0 25.5 41.0 66-67 33.76992006579612 36.5 33.5 39.5 24.5 41.0 68-69 33.29378499922237 36.0 33.0 39.0 23.0 41.0 70-71 32.860548336161244 35.5 32.5 39.0 22.0 40.0 72-73 32.47716862277154 35.0 32.0 38.0 21.5 40.0 74-75 32.024215749419405 35.0 32.0 37.0 20.0 39.5 76-77 31.5981017274118 35.0 31.5 37.0 18.5 39.0 78-79 31.135947458000935 35.0 31.0 36.0 17.5 39.0 80-81 30.6677675889078 34.5 31.0 36.0 12.5 37.5 82-83 30.198018823244098 34.0 30.0 35.0 7.5 37.0 84-85 29.779637031758405 34.0 30.0 35.0 6.0 37.0 86-87 29.363438998436816 34.0 29.0 35.0 2.0 36.0 88-89 28.964449181546506 34.0 29.0 35.0 2.0 36.0 90-91 28.5579331863582 34.0 29.0 35.0 2.0 35.5 92-93 28.123863527526204 34.0 28.5 35.0 2.0 35.0 94-95 27.598856739665095 33.5 27.0 35.0 2.0 35.0 96-97 26.981573043975672 33.0 25.5 35.0 2.0 35.0 98-99 26.240913052991008 33.0 25.0 35.0 2.0 35.0 100-101 24.337330556094972 30.5 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.040059889465982934 1101 2 -0.006332229578795534 1101 3 -0.06752513031980101 1101 4 -0.08077665062402417 1101 5 -0.0465395085294773 1101 6 -0.06272737026325359 1101 7 -0.08741096100131074 1101 8 0.007431065709361917 1101 9 0.027580909506788487 1101 10-11 -0.06790873430178124 1101 12-13 -0.06812103102764411 1101 14-15 -0.04294516933096304 1101 16-17 0.13334155485078725 1101 18-19 0.02591171924725444 1101 20-21 -0.013896340806581975 1101 22-23 0.0736214814529248 1101 24-25 0.006691308993524103 1101 26-27 0.01282999925477668 1101 28-29 0.015615353715254798 1101 30-31 0.0034401763519582573 1101 32-33 -0.08606998766580176 1101 34-35 -0.04008706452470534 1101 36-37 0.05197586019556155 1101 38-39 0.07400505432315185 1101 40-41 0.03697600094978526 1101 42-43 0.08231610010140145 1101 44-45 0.12943369588430187 1101 46-47 0.07194507530940086 1101 48-49 0.03677978043833008 1101 50-51 -0.055149365187205035 1101 52-53 -0.016487671593608866 1101 54-55 0.057802743286195835 1101 56-57 0.09520100791863229 1101 58-59 0.1827787317971783 1101 60-61 0.05982038924201305 1101 62-63 0.023117820866957572 1101 64-65 -0.00808351772671756 1101 66-67 -0.005672512988198264 1101 68-69 0.14991762741723846 1101 70-71 0.1215624620506901 1101 72-73 0.009271553083614492 1101 74-75 0.056066142897019944 1101 76-77 0.20885896355143174 1101 78-79 0.11610854304023377 1101 80-81 0.0904243097695634 1101 82-83 0.03219695806946987 1101 84-85 0.17378178165945357 1101 86-87 0.17724942887412354 1101 88-89 0.18067585047353063 1101 90-91 0.3142268238226862 1101 92-93 0.40381311832288347 1101 94-95 0.45431564334717933 1101 96-97 0.36270912819045975 1101 98-99 0.4214544987864599 1101 100-101 0.8347239985703361 1102 1 0.11918843826708425 1102 2 0.1820323658979781 1102 3 0.20327654551581986 1102 4 0.13275916007614796 1102 5 0.18222688817670019 1102 6 0.003224906394237337 1102 7 0.16344517342754017 1102 8 0.18141988009904253 1102 9 0.16341670709846312 1102 10-11 0.2187928075099066 1102 12-13 0.17600829623565772 1102 14-15 0.29747641127443103 1102 16-17 0.40921533444229397 1102 18-19 0.44437217472336954 1102 20-21 0.32559593567363976 1102 22-23 0.29249837676264434 1102 24-25 0.3568623959308752 1102 26-27 0.3448301373372118 1102 28-29 0.33693360247542614 1102 30-31 0.4098448689156129 1102 32-33 0.22888028166492802 1102 34-35 0.22544723286661394 1102 36-37 0.3575894243691451 1102 38-39 0.4511472796988798 1102 40-41 0.46990936171301456 1102 42-43 0.562117578609751 1102 44-45 0.4371108927362073 1102 46-47 0.452651413619769 1102 48-49 0.3695311616125636 1102 50-51 0.27516444114377947 1102 52-53 0.3321396443777189 1102 54-55 0.3188928854597819 1102 56-57 0.3001996387566379 1102 58-59 0.3519355472616681 1102 60-61 0.3862706396797009 1102 62-63 0.2329155161031622 1102 64-65 0.3185003834473079 1102 66-67 0.4323751693027731 1102 68-69 0.455970349717127 1102 70-71 0.36402822250472155 1102 72-73 0.3447279505221843 1102 74-75 0.4112844694218616 1102 76-77 0.36623552788315195 1102 78-79 0.3748250201467691 1102 80-81 0.4697338229496708 1102 82-83 0.3519135905187518 1102 84-85 0.45660595933422954 1102 86-87 0.3580961354447503 1102 88-89 0.33780055430769806 1102 90-91 0.5640534510612198 1102 92-93 0.5294620241362011 1102 94-95 0.431909528907628 1102 96-97 0.588989807798626 1102 98-99 0.7979036996110374 1102 100-101 1.1820527798482239 1103 1 0.0053238067430676494 1103 2 -0.08858954743831404 1103 3 -0.10082322954936629 1103 4 -0.19144494969813763 1103 5 -0.12574944129090682 1103 6 -0.0878807405933344 1103 7 -0.06799103822069696 1103 8 -0.01818177005780086 1103 9 0.013486987324199617 1103 10-11 0.021120482872404978 1103 12-13 0.05698487711219968 1103 14-15 -0.07341457628654524 1103 16-17 -0.11057335825027792 1103 18-19 -0.14821726114925582 1103 20-21 -0.1255405946163748 1103 22-23 -0.23263613654203397 1103 24-25 -0.3074340113973122 1103 26-27 -0.028561512076834106 1103 28-29 -0.20948743892896005 1103 30-31 -0.18746986460043047 1103 32-33 -0.10450530155619475 1103 34-35 -0.15340203795224028 1103 36-37 0.0574315795532101 1103 38-39 9.709601352696495E-4 1103 40-41 0.05982086386236318 1103 42-43 -0.06141158387469403 1103 44-45 -0.031582790233308344 1103 46-47 0.07686956991613414 1103 48-49 0.03194538259720758 1103 50-51 -0.09629650190638017 1103 52-53 -0.11882075680999549 1103 54-55 -0.032477864634017806 1103 56-57 -0.04140720718491764 1103 58-59 6.665511261232382E-4 1103 60-61 0.005878108429762108 1103 62-63 -0.09586066518554048 1103 64-65 -0.04530891906430412 1103 66-67 0.06955412294639984 1103 68-69 0.05945607749838899 1103 70-71 0.17976380315461427 1103 72-73 0.08548714837530724 1103 74-75 0.005249321690541819 1103 76-77 0.05285019226174015 1103 78-79 -0.09927633574734074 1103 80-81 -0.0434410529171636 1103 82-83 -0.006696356133033987 1103 84-85 -3.5711593036680256E-4 1103 86-87 0.06747835511902167 1103 88-89 -0.010275262628500315 1103 90-91 0.05039299406833919 1103 92-93 0.031186508509801314 1103 94-95 0.01187166519793692 1103 96-97 0.11481002945750163 1103 98-99 0.23877741233061656 1103 100-101 0.5325256000660694 1104 1 -0.015742701415376814 1104 2 -0.06444631364544051 1104 3 -0.05098113736092458 1104 4 -0.12693521817894293 1104 5 -0.04643941737207058 1104 6 -0.0771206441374801 1104 7 0.016054343763855172 1104 8 -0.08105613503048659 1104 9 0.08574876155054767 1104 10-11 0.07363711985630061 1104 12-13 -0.009638015918014275 1104 14-15 0.14246253255952013 1104 16-17 0.1123885877683648 1104 18-19 0.09243097893666885 1104 20-21 0.10285130870561687 1104 22-23 0.08324346775780356 1104 24-25 -0.031200225279839344 1104 26-27 -0.10519774171261531 1104 28-29 -0.03420564028093054 1104 30-31 -0.09160175439819795 1104 32-33 -0.17916965364258175 1104 34-35 -0.1537318881574592 1104 36-37 -0.04351937596166522 1104 38-39 0.07380321760906128 1104 40-41 -0.005963519175146814 1104 42-43 0.14316656060344002 1104 44-45 0.054460455792913365 1104 46-47 0.07848532932586494 1104 48-49 0.11831519533685508 1104 50-51 0.009355663359450261 1104 52-53 0.023449941008948372 1104 54-55 0.1870117418948425 1104 56-57 0.1282050330721205 1104 58-59 0.04108811770070275 1104 60-61 0.084748050632804 1104 62-63 -0.09764960533443201 1104 64-65 -0.022875915431072258 1104 66-67 0.07431190257042886 1104 68-69 0.18790346245046408 1104 70-71 0.1692364593606399 1104 72-73 0.26142020416914846 1104 74-75 0.17503192826518088 1104 76-77 0.19215242586173176 1104 78-79 0.32663664383947477 1104 80-81 0.3306952011102702 1104 82-83 0.32596160984323674 1104 84-85 0.4290100970342827 1104 86-87 0.5463852037699404 1104 88-89 0.34740703871273837 1104 90-91 0.32471447455914415 1104 92-93 0.4976047368051688 1104 94-95 0.5450743343202795 1104 96-97 0.6289561564757271 1104 98-99 0.6741197360521376 1104 100-101 0.9180585727031385 1105 1 0.1028430819733579 1105 2 0.1042719028417487 1105 3 0.10895128758073014 1105 4 0.09350715604695381 1105 5 0.1058316479920336 1105 6 0.06025133863673915 1105 7 0.140156306048425 1105 8 0.18529494393594348 1105 9 0.243981183217997 1105 10-11 0.20283550182311672 1105 12-13 0.1777839703314612 1105 14-15 0.23375520712220066 1105 16-17 0.15879732726146756 1105 18-19 0.2405690746917415 1105 20-21 0.17557462836195015 1105 22-23 0.19183573637612739 1105 24-25 0.2751820678031365 1105 26-27 0.31647042132455994 1105 28-29 0.22681522906150775 1105 30-31 0.21972344396424148 1105 32-33 0.16153926409381114 1105 34-35 0.1700226634225146 1105 36-37 0.11576251554256345 1105 38-39 0.26287781727967996 1105 40-41 0.10046992622800843 1105 42-43 0.26605649149467325 1105 44-45 0.08877128382863475 1105 46-47 0.13810908835794322 1105 48-49 0.2152110737982511 1105 50-51 0.1290828241787878 1105 52-53 0.07479770842988387 1105 54-55 0.18873256798327276 1105 56-57 0.17992416461428462 1105 58-59 0.25735343539366085 1105 60-61 0.19646661349673167 1105 62-63 0.144420492587372 1105 64-65 0.2888311302625155 1105 66-67 0.2977145629506168 1105 68-69 0.29461593356419513 1105 70-71 0.36293515984471014 1105 72-73 0.27100989994927005 1105 74-75 0.21505122311565472 1105 76-77 0.24650442275525464 1105 78-79 0.17372452300855912 1105 80-81 0.33681366195677853 1105 82-83 0.3877707591293138 1105 84-85 0.44806656218780816 1105 86-87 0.38260658963305616 1105 88-89 0.32766959573134713 1105 90-91 0.33614821004564277 1105 92-93 0.4399512468153439 1105 94-95 0.5143074056595296 1105 96-97 0.5933176945263305 1105 98-99 0.6796578866071137 1105 100-101 1.1325459092727677 1106 1 -0.011011245659467761 1106 2 0.03883704852923486 1106 3 -0.1266403577550932 1106 4 -0.11261683519627752 1106 5 -0.12286077070535129 1106 6 -0.05639915555532582 1106 7 -0.1034730272218809 1106 8 -0.18187804149435038 1106 9 -0.1443647548504785 1106 10-11 -0.4257770340059963 1106 12-13 -0.4261643705898379 1106 14-15 -0.410041621454468 1106 16-17 -0.39503657130997283 1106 18-19 -0.09557059288565739 1106 20-21 -0.22406723490302483 1106 22-23 -0.05049005711318699 1106 24-25 -0.15899319242038956 1106 26-27 -0.2249834958532091 1106 28-29 -0.057613350245688366 1106 30-31 -0.04184562463962749 1106 32-33 -2.2604615046617482E-4 1106 34-35 -0.07919362633712268 1106 36-37 -0.08107635657052725 1106 38-39 -0.1104879270129473 1106 40-41 -0.22393958680697068 1106 42-43 -0.1514965204716745 1106 44-45 0.04782622999739772 1106 46-47 0.20053185920217942 1106 48-49 0.17030371045672865 1106 50-51 0.13803959629339602 1106 52-53 -0.08436286454630704 1106 54-55 0.030738617063697404 1106 56-57 0.013777167786393818 1106 58-59 0.08001224345141367 1106 60-61 0.01625355631558989 1106 62-63 0.16420137738249707 1106 64-65 0.23630391227522551 1106 66-67 0.2293531721305797 1106 68-69 -0.005411311572409261 1106 70-71 0.1823512749543994 1106 72-73 0.3075677840998381 1106 74-75 0.08685593500179678 1106 76-77 0.34612787771964904 1106 78-79 0.3431710134112258 1106 80-81 0.3276041350383885 1106 82-83 0.16774051585654348 1106 84-85 0.05868410584124817 1106 86-87 0.18890580575246574 1106 88-89 0.23808728848243632 1106 90-91 0.3302743757893367 1106 92-93 0.43494113342915597 1106 94-95 0.41382358356398896 1106 96-97 0.45319001608665843 1106 98-99 0.5617777945932936 1106 100-101 0.7948070405453507 1107 1 -0.04716709491605542 1107 2 -0.012584543966415396 1107 3 -0.00885939966084237 1107 4 -0.05615216943886736 1107 5 -0.02086654190370041 1107 6 0.011873107631402036 1107 7 0.006106884058048934 1107 8 0.03219275163293389 1107 9 0.003751311157827786 1107 10-11 0.06665835610136384 1107 12-13 0.0845876920576103 1107 14-15 0.022470732909305013 1107 16-17 0.10143029586010499 1107 18-19 -0.05303905350188387 1107 20-21 -0.15489064394522245 1107 22-23 0.04068631325498728 1107 24-25 0.00995301240693891 1107 26-27 0.09079727034600893 1107 28-29 0.043702520639442355 1107 30-31 0.08593158522449329 1107 32-33 0.012976152930768592 1107 34-35 0.015018429333956362 1107 36-37 -0.09166589129680602 1107 38-39 0.026806021209466735 1107 40-41 0.0040577194888911094 1107 42-43 -0.05958104037092227 1107 44-45 -0.04263356037072441 1107 46-47 -0.009481564483230898 1107 48-49 -0.13292664966927958 1107 50-51 -0.08259317758520268 1107 52-53 -0.005997841630986045 1107 54-55 0.06401133308684592 1107 56-57 0.03526078093675977 1107 58-59 0.1241021453786999 1107 60-61 0.19741849816870882 1107 62-63 0.0300493740969614 1107 64-65 0.16550686189919617 1107 66-67 0.11118898440702196 1107 68-69 0.08154202296251611 1107 70-71 0.12385332885897071 1107 72-73 -0.049766439662327855 1107 74-75 0.07744837057124343 1107 76-77 0.03527114709096324 1107 78-79 0.19175238004462258 1107 80-81 0.30953394449737814 1107 82-83 0.320387972395455 1107 84-85 0.5158071105588142 1107 86-87 0.3956009697285694 1107 88-89 0.4110450186690535 1107 90-91 0.49024421957422604 1107 92-93 0.579954149113437 1107 94-95 0.483646699383403 1107 96-97 0.4751170502412805 1107 98-99 0.5952140473316057 1107 100-101 0.7885084671145641 1108 1 0.016508340329263405 1108 2 -0.007772286148373553 1108 3 -0.01802229147259382 1108 4 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50-51 -1.0450189681753628 2316 52-53 -0.8241012843695046 2316 54-55 -1.0413700434625852 2316 56-57 -1.263863760799559 2316 58-59 -1.2498040837956879 2316 60-61 -1.3065493065495488 2316 62-63 -1.2389895447941797 2316 64-65 -1.2676518775886407 2316 66-67 -1.2855443161673819 2316 68-69 -1.093956707629026 2316 70-71 -1.2395214669999177 2316 72-73 -1.3205312241358769 2316 74-75 -1.0404966169113088 2316 76-77 -1.3375720227528411 2316 78-79 -1.1450226480172532 2316 80-81 -1.1815850137687072 2316 82-83 -1.6427259162659418 2316 84-85 -1.6225574079893228 2316 86-87 -1.468967359132531 2316 88-89 -1.6772800366116378 2316 90-91 -1.8176162370274191 2316 92-93 -1.8863671838537677 2316 94-95 -2.025557917056119 2316 96-97 -2.311971395652691 2316 98-99 -2.361761155444551 2316 100-101 -3.4083070519458296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8752.0 3 1710.0 4 1507.0 5 1632.0 6 1851.0 7 2115.0 8 2367.0 9 3072.0 10 3911.0 11 5108.0 12 6851.0 13 8558.0 14 9982.0 15 12244.0 16 15218.0 17 19562.0 18 16827.0 19 12245.0 20 11877.0 21 12407.0 22 13473.0 23 15024.0 24 16915.0 25 19280.0 26 22106.0 27 26449.0 28 31912.0 29 39463.0 30 48691.0 31 62712.0 32 80731.0 33 108420.0 34 150579.0 35 219993.0 36 333007.0 37 476903.0 38 395788.0 39 56868.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.95445618925828 19.01037637330787 14.91968989459643 23.11547754283742 2 15.093338667768801 19.806735400397706 40.71814837514888 24.381777556684618 3 18.98731165531833 24.090715628279703 29.801968768642805 27.120003947759162 4 12.609330537996573 16.299448247054187 37.66905750797314 33.42216370697609 5 13.601720214057375 37.130582488978774 34.7463851931165 14.521312103847347 6 32.08324399196742 36.29154153371864 17.385308801184898 14.239905673129039 7 28.398899405679067 32.42267224301123 20.94373761831389 18.234690732995816 8 26.217494766362957 34.89069348167291 19.934312626988618 18.95749912497551 9 25.793174918468114 16.649321330713043 20.577423592474414 36.98008015834443 10-11 24.55423044330483 26.689525458439938 27.647498761032647 21.108745337222587 12-13 25.47557068151574 23.84650183430396 28.183604305468567 22.494323178711728 14-15 22.47062669292058 25.30946120412123 26.367923853381658 25.85198824957653 16-17 21.88492174868224 28.51365972261204 27.082451430690984 22.51896709801474 18-19 21.85797449747425 27.159492150673092 29.488239161734718 21.49429419011794 20-21 22.367916839173702 26.258420929488807 29.749743192877943 21.623919038459547 22-23 22.12580194220403 26.10151151536949 29.6141102648382 22.158576277588278 24-25 21.997785022136572 26.23782576505212 29.734999379348416 22.029389833462893 26-27 21.723694410000224 26.619373542173154 29.56731457014714 22.089617477679486 28-29 21.75866023610521 26.81810477471389 29.504368364631322 21.918866624549572 30-31 21.85790882062538 26.494084028914454 29.474535842011818 22.173471308448352 32-33 21.745604235343325 26.47276689652136 29.45234413265166 22.329284735483657 34-35 22.199705479538004 26.591644451411057 29.25230153277008 21.956348536280856 36-37 22.1700923656637 26.360960127482887 29.315384776228136 22.153562730625275 38-39 21.86612118014723 26.654028740607117 29.303591288326736 22.17625879091892 40-41 22.138350633178288 26.5807007944826 29.174489048958247 22.106459523380874 42-43 21.86214714136356 26.50162328729434 29.159104165520276 22.477125405821823 44-45 22.053769925997017 26.685603976174647 29.132324908761937 22.1283011890664 46-47 22.267656265924753 26.887796622780108 28.554548038026358 22.289999073268778 48-49 22.141029343909494 26.954367420546728 28.466669777992244 22.43793345755154 50-51 22.248483610310746 27.160260903563955 28.39190924205172 22.199346244073574 52-53 22.3134434343957 27.10663788082795 28.237586657521547 22.342332027254802 54-55 22.15416755639404 27.177579310481466 28.274244428839058 22.39400870428544 56-57 22.142346707126755 27.363291493323665 28.185962347732712 22.30839945181686 58-59 22.17810098303334 27.100288239363017 28.266274453963902 22.455336323639745 60-61 22.1192272471886 27.170206458709252 28.408192854129403 22.302373439972744 62-63 22.020843361375537 27.194641850604896 28.573928212753625 22.210586575265946 64-65 21.94095055620881 27.08099915336803 28.46309778390176 22.5149525065214 66-67 21.901951459231466 27.207113309109094 28.251670964338604 22.639264267320844 68-69 22.01936253242365 27.024581345148707 28.49132849647799 22.464727625949656 70-71 22.03065479832416 26.959623455089194 28.361718873111975 22.648002873474677 72-73 22.023800746494164 27.043675784982035 28.221696309940114 22.710827158583683 74-75 21.93803885524322 27.343406784480795 28.090904048661052 22.62765031161493 76-77 22.15210490612849 27.066553269864578 28.14626602731296 22.635075796693975 78-79 22.100690264154466 27.100516388546396 28.201347073761557 22.597446273537585 80-81 22.091221771484022 27.23101652040725 28.007679692636216 22.670082015472516 82-83 22.11019996742771 27.22402690294779 28.04746750474279 22.618305624881703 84-85 22.11974721445817 27.26709358025338 27.982088378990817 22.631070826297638 86-87 22.135690722511615 27.176744769487744 28.018534290197422 22.66903021780322 88-89 22.16230360385401 27.046125818975202 27.960904421954424 22.830666155216363 90-91 22.104862368966057 27.040388056350924 28.039140166429338 22.815609408253682 92-93 22.11545063848742 26.859650728983087 28.166578048990903 22.858320583538593 94-95 22.009673938662765 26.94820664173834 28.13600292784203 22.906116491756862 96-97 22.06184886377141 26.889510957354567 28.002654060977385 23.045986117896646 98-99 21.84450837371464 27.110969959038755 27.87675663350547 23.16776503374114 100-101 22.135756962248166 26.670202348073897 28.08043308429519 23.113607605382743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2928.0 1 1649.5 2 417.0 3 451.5 4 373.0 5 359.0 6 616.0 7 1220.5 8 2065.0 9 2503.0 10 2307.5 11 2100.5 12 2143.0 13 2286.0 14 2451.0 15 2601.0 16 2719.5 17 2868.5 18 3122.0 19 3454.0 20 3896.0 21 4557.5 22 5402.5 23 6797.0 24 8569.5 25 10708.5 26 14236.5 27 18423.0 28 21905.0 29 25273.0 30 28945.5 31 33306.0 32 39211.0 33 43093.0 34 46094.5 35 50362.5 36 55055.5 37 60061.5 38 63228.5 39 65116.5 40 67569.5 41 70907.5 42 73025.5 43 75524.0 44 79238.5 45 81791.0 46 82935.5 47 84273.5 48 85449.0 49 84665.0 50 82212.5 51 78886.5 52 77476.5 53 77084.5 54 75341.0 55 69780.0 56 62931.0 57 57830.5 58 53453.5 59 47416.0 60 38401.0 61 31307.5 62 25632.5 63 18910.0 64 13378.5 65 10059.0 66 7426.0 67 5523.0 68 4361.5 69 3404.5 70 2481.0 71 1667.0 72 1198.0 73 894.5 74 640.0 75 451.5 76 328.0 77 226.0 78 150.0 79 106.0 80 72.5 81 44.5 82 37.0 83 34.0 84 26.0 85 17.0 86 17.5 87 16.5 88 12.5 89 9.5 90 9.5 91 10.0 92 8.5 93 8.5 94 7.0 95 6.5 96 8.5 97 10.5 98 8.5 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3340330614967036 2 0.28957147288238505 3 0.28469475713516235 4 0.29119704479812597 5 0.29159245418303587 6 0.21400434598848392 7 0.20306468633930871 8 0.20899582711295792 9 0.2028889488349043 10-11 0.22307679465336216 12-13 0.21196139749978254 14-15 0.20712861612866093 16-17 0.20888599117270518 18-19 0.20304271915125816 20-21 0.19662830024049677 22-23 0.19891288779775423 24-25 0.18962076725237048 26-27 0.19036765164608926 28-29 0.20548107702486948 30-31 0.2170138507514096 32-33 0.2091056630532107 34-35 0.2184636851627461 36-37 0.1952663345813625 38-39 0.19280600951970062 40-41 0.19019191414168485 42-43 0.19757288932667053 44-45 0.20174665505627554 46-47 0.20638173173494215 48-49 0.21308172409036072 50-51 0.216947949187258 52-53 0.2341482574308407 54-55 0.21207123344003528 56-57 0.18687486874605141 58-59 0.18564470621522045 60-61 0.18305257802525524 62-63 0.1918833876215774 64-65 0.1835797905384685 66-67 0.18256929988814308 68-69 0.17808799352583032 70-71 0.18926929224356165 72-73 0.18902765317500556 74-75 0.19366272985367217 76-77 0.19563977677822192 78-79 0.19256437045114455 80-81 0.19403617205053156 82-83 0.18680896718189974 84-85 0.17914241855225688 86-87 0.18087782640825054 88-89 0.18239356238373866 90-91 0.18834667034543842 92-93 0.1840850358636312 94-95 0.1819542186227276 96-97 0.1815368420497671 98-99 0.19069715946684757 100-101 0.17780242008117317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.41880541081405 #Duplication Level Percentage of deduplicated Percentage of total 1 74.77196761750749 40.690011559608294 2 13.788564523071809 15.007144193509975 3 4.856275241084631 7.928180920978159 4 2.1884246126588787 4.763658126100785 5 1.2070229568013233 3.2842373706278316 6 0.7271160422920689 2.3741271849944003 7 0.47604110745516814 1.8133911875905855 8 0.3128508408728154 1.3619975225653833 9 0.24472471090802908 1.1985863779909582 >10 1.30072955863558 12.751956497166065 >50 0.08581928189378168 3.1845246012434907 >100 0.03796241317873757 3.787052479799953 >500 0.0016136087997334777 0.5557975932425817 >1k 7.261239598798148E-4 0.6775031725510603 >5k 1.613608799732922E-4 0.6218312120303717 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8278 0.36368876536494965 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5885 0.2585538033550047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4226 0.185666673403271 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3192 0.14023852851472812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.39343761011053E-5 8.78687522022106E-5 14-15 0.0 0.0 0.0 4.39343761011053E-5 1.0983594025276326E-4 16-17 0.0 0.0 0.0 4.39343761011053E-5 1.757375044044212E-4 18-19 0.0 0.0 0.0 4.39343761011053E-5 1.757375044044212E-4 20-21 0.0 0.0 0.0 4.39343761011053E-5 1.757375044044212E-4 22-23 0.0 0.0 0.0 1.0983594025276326E-4 2.4163906855607914E-4 24-25 0.0 0.0 0.0 2.1967188050552651E-4 3.075406327077371E-4 26-27 0.0 0.0 0.0 3.295078207582897E-4 3.075406327077371E-4 28-29 0.0 0.0 0.0 7.029500176176848E-4 3.954093849099477E-4 30-31 0.0 0.0 0.0 0.0019331125484486331 3.954093849099477E-4 32-33 0.0 0.0 0.0 0.004679011054767715 3.954093849099477E-4 34-35 0.0 0.0 0.0 0.007402942373036243 4.3934376101105303E-4 36-37 0.0 0.0 0.0 0.011576708102641248 4.3934376101105303E-4 38-39 0.0 0.0 0.0 0.015926211336650672 4.3934376101105303E-4 40-41 0.0 0.0 0.0 0.022538334939867018 4.3934376101105303E-4 42-43 0.0 0.0 0.0 0.03220389768211018 4.3934376101105303E-4 44-45 0.0 0.0 0.0 0.04689994648792991 4.6131094906160566E-4 46-47 0.0 0.0 0.0 0.07380975184985691 4.832781371121583E-4 48-49 0.0 0.0 0.0 0.11218642937417239 4.832781371121583E-4 50-51 0.0 0.0 0.0 0.15001392719722406 4.832781371121583E-4 52-53 0.0 0.0 0.0 0.18951093131211771 4.832781371121583E-4 54-55 0.0 0.0 0.0 0.2374433356384236 4.832781371121583E-4 56-57 0.0 0.0 0.0 0.29113114323397427 4.832781371121583E-4 58-59 0.0 0.0 0.0 0.35668123237682337 4.832781371121583E-4 60-61 0.0 0.0 0.0 0.4413427751236533 4.832781371121583E-4 62-63 0.0 0.0 0.0 0.5277177585384263 5.711468893143689E-4 64-65 0.0 0.0 0.0 0.6276245297923397 6.370484534660269E-4 66-67 0.0 0.0 0.0 0.7614706065843571 6.590156415165795E-4 68-69 0.0 0.0 0.0 0.9139668260312935 6.590156415165795E-4 70-71 0.0 0.0 0.0 1.080653848958887 6.590156415165795E-4 72-73 0.0 0.0 0.0 1.2583903674759087 7.249172056682375E-4 74-75 0.0 0.0 0.0 1.4389606532514514 7.468843937187902E-4 76-77 0.0 2.196718805055265E-5 0.0 1.6373243613479418 7.468843937187902E-4 78-79 0.0 4.39343761011053E-5 0.0 1.871494585966833 7.468843937187902E-4 80-81 0.0 4.39343761011053E-5 0.0 2.118889057792157 7.468843937187902E-4 82-83 0.0 4.39343761011053E-5 0.0 2.4081529900418346 7.468843937187902E-4 84-85 0.0 4.39343761011053E-5 0.0 2.724634268286146 7.468843937187902E-4 86-87 0.0 4.39343761011053E-5 0.0 3.106292193476448 7.468843937187902E-4 88-89 0.0 4.39343761011053E-5 0.0 3.568965108197188 7.468843937187902E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4180 0.0 47.7866 1 GTATCAA 7535 0.0 43.23549 1 ATCAACG 9515 0.0 33.774517 3 TCAACGC 9630 0.0 33.372654 4 TATCAAC 9760 0.0 33.121525 2 CAACGCA 9770 0.0 32.797115 5 AACGCAG 10080 0.0 31.856125 6 CGCAGAG 11440 0.0 27.529852 8 ACGCAGA 11585 0.0 27.471703 7 TACATGG 7325 0.0 24.597038 2 GCAGAGT 12690 0.0 24.143225 9 GTACATG 7545 0.0 24.141966 1 GTATAGG 715 0.0 23.94581 1 ACATGGG 7430 0.0 23.097746 3 GAGTACT 7565 0.0 21.913359 12-13 AGAGTAC 11325 0.0 21.474321 10-11 GTACTTT 8085 0.0 20.2674 14-15 AGTACTT 8165 0.0 20.215807 12-13 CAGAGTA 12065 0.0 20.176888 10-11 TCCGTTG 270 2.300294E-8 19.352865 8 >>END_MODULE