FastQCFastQC Report
Thu 26 May 2016
SRR937162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276122
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86620.3805595657877741No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69210.3040698169957498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49780.2187053242313022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.049.2411041
GTATCAA82500.041.3525621
ATCAACG104700.032.3014563
TCAACGC105250.032.0875324
TATCAAC107150.031.6515392
CAACGCA108250.031.2421475
AACGCAG111450.030.3863956
TACCGTG2104.3655746E-1127.1455387
TAAGGTG9450.026.6399155
ACGCAGA127700.026.374967
CGCAGAG127400.026.2879168
GCAGAGT140300.023.49849
GTAAGGT9350.022.860714
GAGTACT85550.022.68341812-13
GTACATG75200.021.8620151
TGGTATC23100.021.7962952
TACATGG74650.021.5067942
AGAGTAC124100.021.30246510-11
AGTACTT89400.021.22832712-13
GTACTTT90950.021.1766814-15
Produced by FastQC (version 0.11.5)