##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937162_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276122 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03963451871209 31.0 31.0 34.0 30.0 34.0 2 32.29109555638933 33.0 31.0 34.0 31.0 34.0 3 32.36644301140273 34.0 31.0 34.0 31.0 34.0 4 36.02484620771646 37.0 35.0 37.0 35.0 37.0 5 35.86580684163678 37.0 35.0 37.0 35.0 37.0 6 35.952615457343676 37.0 35.0 37.0 35.0 37.0 7 35.91139930109195 37.0 35.0 37.0 35.0 37.0 8 35.884341876226316 37.0 35.0 37.0 35.0 37.0 9 37.5648858013762 39.0 37.0 39.0 35.0 39.0 10-11 37.47503604815559 39.0 37.0 39.0 35.0 39.0 12-13 37.384552321887845 39.0 37.0 39.0 34.0 39.0 14-15 38.568362987572726 40.0 38.0 41.0 34.0 41.0 16-17 38.53761243905204 40.0 38.0 41.0 34.0 41.0 18-19 38.527724788038604 40.0 38.0 41.0 34.0 41.0 20-21 38.41930221666502 40.0 38.0 41.0 34.0 41.0 22-23 38.2768401693758 40.0 38.0 41.0 34.0 41.0 24-25 38.1274571398194 40.0 37.5 41.0 33.0 41.0 26-27 37.88114015856795 40.0 37.0 41.0 33.0 41.0 28-29 37.871369812338706 40.0 37.0 41.0 33.0 41.0 30-31 38.01925006656057 40.0 37.0 41.0 33.0 41.0 32-33 37.992698106691996 40.0 37.0 41.0 33.0 41.0 34-35 37.87316541028996 40.0 37.0 41.0 33.0 41.0 36-37 37.70653242664497 40.0 37.0 41.0 33.0 41.0 38-39 37.5514752724151 40.0 37.0 41.0 33.0 41.0 40-41 37.3850463639471 40.0 37.0 41.0 32.0 41.0 42-43 37.17718206669062 40.0 36.0 41.0 31.5 41.0 44-45 36.909281224820106 39.0 36.0 41.0 31.0 41.0 46-47 36.606742081487724 39.0 36.0 41.0 30.5 41.0 48-49 36.29860350192125 39.0 35.5 40.5 30.0 41.0 50-51 36.08076763899299 39.0 35.0 40.0 29.5 41.0 52-53 35.834739306592525 39.0 35.0 40.0 29.0 41.0 54-55 35.61403606660803 38.5 35.0 40.0 28.0 41.0 56-57 35.34826011083764 38.0 34.5 40.0 28.0 41.0 58-59 35.07466032137118 38.0 34.0 40.0 27.5 41.0 60-61 34.71872399634115 38.0 34.0 40.0 26.5 41.0 62-63 34.3161557245174 37.0 33.5 40.0 26.0 41.0 64-65 33.946938037592005 36.5 33.0 39.0 26.0 41.0 66-67 33.54338124230599 36.0 33.0 39.0 25.0 40.0 68-69 33.07754395414657 35.5 32.0 39.0 24.0 40.0 70-71 32.689625160689985 35.0 32.0 38.0 23.5 40.0 72-73 32.24433444252988 35.0 31.0 37.5 22.0 39.5 74-75 31.786494968195903 35.0 31.0 37.0 21.5 39.0 76-77 30.52681578579707 33.5 29.5 35.5 20.0 38.0 78-79 31.179324306869315 34.0 31.0 36.0 20.5 38.0 80-81 31.01629174534581 34.0 31.0 35.5 20.0 37.0 82-83 30.66416497006751 34.0 31.0 35.0 18.5 37.0 84-85 30.31453410669551 34.0 30.0 35.0 18.0 36.5 86-87 29.953832878905438 34.0 30.0 35.0 15.5 36.0 88-89 29.550808128914007 34.0 29.5 35.0 8.5 36.0 90-91 29.046246422643428 34.0 29.0 35.0 4.5 35.0 92-93 28.571448938150063 33.5 29.0 35.0 2.0 35.0 94-95 28.102496263381312 33.0 28.5 35.0 2.0 35.0 96-97 27.600237597105956 33.0 27.0 35.0 2.0 35.0 98-99 26.841051138735093 33.0 25.5 34.0 2.0 35.0 100-101 25.129312488522146 31.0 21.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.050421196103766164 1101 2 0.022616819691840817 1101 3 0.022739475011647414 1101 4 -0.06932969243925413 1101 5 -0.11505299942179903 1101 6 -0.07495495266719132 1101 7 -0.06893405240902695 1101 8 0.0014902718618117206 1101 9 -0.110292392431802 1101 10-11 -0.04245804609358572 1101 12-13 0.05551426085173006 1101 14-15 0.06984966307908991 1101 16-17 -0.05190954731133246 1101 18-19 0.0449927725959256 1101 20-21 -0.08497294125805865 1101 22-23 -0.10929838150607196 1101 24-25 -0.05226359921191914 1101 26-27 -0.13603034396588498 1101 28-29 -0.1053238700870267 1101 30-31 -0.1091440126086951 1101 32-33 -0.08829589442918007 1101 34-35 -0.08522234897785097 1101 36-37 -0.17646332988820745 1101 38-39 -0.18107229458832563 1101 40-41 -0.1406245077701982 1101 42-43 -0.2014604603094412 1101 44-45 -0.10826673690068134 1101 46-47 -0.1496598842143939 1101 48-49 -0.2041669785710596 1101 50-51 -0.11121924080578793 1101 52-53 -0.13958783876134362 1101 54-55 -0.14893999394580248 1101 56-57 -0.288878853853312 1101 58-59 -0.3158755683677086 1101 60-61 -0.2758881631445931 1101 62-63 -0.30642418298435103 1101 64-65 -0.23752034046247417 1101 66-67 -0.275434936006981 1101 68-69 -0.3394121661280849 1101 70-71 -0.3965596294395155 1101 72-73 -0.4796256420473881 1101 74-75 -0.46628693617523354 1101 76-77 -0.3980169171108763 1101 78-79 -0.36102078290750583 1101 80-81 -0.4576751134045658 1101 82-83 -0.4614976579019121 1101 84-85 -0.3760665980808007 1101 86-87 -0.5785432917969118 1101 88-89 -0.4521553720721059 1101 90-91 -0.3256520189357772 1101 92-93 -0.32719439595785005 1101 94-95 -0.5322985960353854 1101 96-97 -0.6287613119506865 1101 98-99 -0.7277080331667385 1101 100-101 -0.4004609239653263 1102 1 0.13893852590270228 1102 2 0.13384772664620215 1102 3 0.037596865244267974 1102 4 0.0703013195697153 1102 5 -0.07783399834559646 1102 6 -0.02265520368799656 1102 7 0.04764745564710893 1102 8 0.1090005102546101 1102 9 0.08773060295876434 1102 10-11 0.16231727372352367 1102 12-13 0.1262807629857079 1102 14-15 0.10212008820039387 1102 16-17 0.1338739328978633 1102 18-19 0.07345058304699847 1102 20-21 0.1659068911024022 1102 22-23 0.10713734695108457 1102 24-25 0.14321256827273743 1102 26-27 0.14598129321132092 1102 28-29 0.0595795398494019 1102 30-31 0.12367972189610299 1102 32-33 0.006822891453012403 1102 34-35 0.09246157006292322 1102 36-37 0.03513106974942559 1102 38-39 0.017173731926298785 1102 40-41 -0.01713799156674156 1102 42-43 0.08143289853182267 1102 44-45 0.09613566703988852 1102 46-47 0.024495888634405105 1102 48-49 0.15816180037435856 1102 50-51 0.169832676790719 1102 52-53 0.23138210171860152 1102 54-55 0.1625285454172598 1102 56-57 0.1275433929065315 1102 58-59 0.09141299173570161 1102 60-61 -0.062057387835032785 1102 62-63 0.21514149457356524 1102 64-65 0.1742655552563619 1102 66-67 0.13438673705766035 1102 68-69 0.04890522008336262 1102 70-71 -0.08310960711774129 1102 72-73 -0.03629458054331991 1102 74-75 0.003822694748141231 1102 76-77 0.011932528856672064 1102 78-79 0.11579872142320013 1102 80-81 0.13398060229862807 1102 82-83 0.05377318659813568 1102 84-85 0.037729423372358184 1102 86-87 0.02437906435594428 1102 88-89 0.2921811254948885 1102 90-91 0.19753659374158872 1102 92-93 0.13420199999726634 1102 94-95 0.14094601933272344 1102 96-97 0.20228503103395568 1102 98-99 0.12645601529832717 1102 100-101 0.1357038457005757 1103 1 -0.05900115880763224 1103 2 -0.06408948206702547 1103 3 0.004971794059350998 1103 4 0.02759646413937844 1103 5 -0.08216880267516302 1103 6 -0.03522012718920564 1103 7 0.007333842043600214 1103 8 -0.018360019684955375 1103 9 -0.0053620317116696015 1103 10-11 0.013257962165006631 1103 12-13 0.03870817341650934 1103 14-15 -0.022883625795273588 1103 16-17 -0.11890842124210366 1103 18-19 -0.10891874620538289 1103 20-21 -0.1838389382709238 1103 22-23 -0.1907453898256577 1103 24-25 -0.11157505783645405 1103 26-27 -0.09957324443431048 1103 28-29 -0.2018352353776649 1103 30-31 -0.1904977899553515 1103 32-33 -0.06477946912454513 1103 34-35 -0.030060861518613535 1103 36-37 -0.1601474418334874 1103 38-39 -0.15695695501053564 1103 40-41 -0.1413154367567344 1103 42-43 -0.22862383989431834 1103 44-45 -0.30182120680424873 1103 46-47 -0.2977429936624034 1103 48-49 -0.42398011806629654 1103 50-51 -0.30117063533157307 1103 52-53 -0.24676874745318145 1103 54-55 -0.3164150989838461 1103 56-57 -0.3289243400895643 1103 58-59 -0.4475081591839398 1103 60-61 -0.4549645131833415 1103 62-63 -0.22448126822020242 1103 64-65 -0.3789975232460776 1103 66-67 -0.3956856763348213 1103 68-69 -0.4041631117231148 1103 70-71 -0.4453867435699479 1103 72-73 -0.42452716618901576 1103 74-75 -0.5603878621416989 1103 76-77 -0.7462295956250351 1103 78-79 -0.3900572022094302 1103 80-81 -0.4110987343626995 1103 82-83 -0.40563574527575597 1103 84-85 -0.4366007717933549 1103 86-87 -0.3133752253090556 1103 88-89 -0.31124908281265107 1103 90-91 -0.13776812531844485 1103 92-93 -0.18789141746918148 1103 94-95 -0.27665903436784234 1103 96-97 -0.20070016440792315 1103 98-99 -0.26289900649996767 1103 100-101 0.04808806902789797 1104 1 -0.05292182063949724 1104 2 0.05706554903046168 1104 3 0.0021843909598899813 1104 4 0.02707298173176298 1104 5 -0.13428870003508564 1104 6 -0.08470929091574675 1104 7 -0.0827482865296858 1104 8 -0.13940416257351274 1104 9 -0.0617283283006671 1104 10-11 -0.07242128534122827 1104 12-13 -0.04953182244066312 1104 14-15 -0.08840851916622938 1104 16-17 -0.13362653253258827 1104 18-19 -0.06730330201086332 1104 20-21 -0.11503833966222743 1104 22-23 -0.1944217748775543 1104 24-25 -0.17088899192495433 1104 26-27 -0.1796407230053063 1104 28-29 -0.18453526757465255 1104 30-31 -0.194533764266815 1104 32-33 -0.057349114800132384 1104 34-35 -0.27377175513122154 1104 36-37 -0.09216630896501954 1104 38-39 -0.19193177156653718 1104 40-41 -0.1354483059731919 1104 42-43 0.002883909521592898 1104 44-45 0.0025019135561805683 1104 46-47 -0.007228926433008098 1104 48-49 -0.23970802504478428 1104 50-51 -0.14603896918650605 1104 52-53 -0.019865579315137438 1104 54-55 0.07095531566675817 1104 56-57 -0.05887805974165161 1104 58-59 -0.06970508913786233 1104 60-61 -0.2577861396488075 1104 62-63 -0.0941635125224991 1104 64-65 -0.04415828675735867 1104 66-67 -0.14565434244160258 1104 68-69 -0.2749385711680432 1104 70-71 -0.22675084491013564 1104 72-73 -0.1556458687331812 1104 74-75 -0.18821053932454745 1104 76-77 -0.053937433151109104 1104 78-79 -0.042787979147387745 1104 80-81 0.004315889972065889 1104 82-83 -0.12734002011940504 1104 84-85 -0.15207044621356403 1104 86-87 -0.10158493799038482 1104 88-89 -0.08919216094925275 1104 90-91 -0.01395036999599597 1104 92-93 -0.16567249750157131 1104 94-95 -0.19105061647052324 1104 96-97 -0.22091052553198054 1104 98-99 -0.08810278938273441 1104 100-101 0.14026459630189336 1105 1 -0.0010603759325462647 1105 2 0.026491626316705208 1105 3 0.015257958967772822 1105 4 0.05094971296926332 1105 5 -0.07142275702583589 1105 6 -0.027520365091490362 1105 7 0.006732946801292883 1105 8 0.012141407221974987 1105 9 -0.041472756259189225 1105 10-11 0.05254331587459404 1105 12-13 -0.06279322659339215 1105 14-15 0.03928013696668131 1105 16-17 0.08635041589403869 1105 18-19 0.005021799274182115 1105 20-21 0.006317021623651442 1105 22-23 0.03561445969130972 1105 24-25 -0.05180001446012028 1105 26-27 -0.06493961511671387 1105 28-29 -0.05445485991852195 1105 30-31 -0.04224072724356631 1105 32-33 -0.013892188730025623 1105 34-35 0.019623994946755374 1105 36-37 0.04162486779109287 1105 38-39 0.10696206309974343 1105 40-41 0.14542805016643 1105 42-43 -0.117169954072601 1105 44-45 0.0613290922400509 1105 46-47 0.018360014556002113 1105 48-49 -0.041986500458641274 1105 50-51 0.023214465428992526 1105 52-53 0.0393846615114839 1105 54-55 0.12644596148418685 1105 56-57 0.24527389452972415 1105 58-59 0.18322719392143227 1105 60-61 0.11317258292753962 1105 62-63 0.09673071389989474 1105 64-65 0.1650469480694099 1105 66-67 0.06256360377982162 1105 68-69 0.1842444047883518 1105 70-71 -0.04336862822534471 1105 72-73 0.026243423974698032 1105 74-75 -0.027804790274785773 1105 76-77 0.14129813462815832 1105 78-79 0.16577304088904654 1105 80-81 0.18987330091217203 1105 82-83 0.09342417429042982 1105 84-85 0.02944231257093577 1105 86-87 0.07361017895915367 1105 88-89 0.2146096828319628 1105 90-91 0.05596158252913597 1105 92-93 0.06893068284582782 1105 94-95 0.04177027458508675 1105 96-97 -0.026137367834056846 1105 98-99 0.02455998040195695 1105 100-101 0.12840420853226675 1106 1 -0.20807880403615187 1106 2 -0.09158255668586435 1106 3 -0.14987588928693185 1106 4 -0.052798468292770906 1106 5 -0.17464402863163286 1106 6 -0.12925758058529624 1106 7 -0.1074175745400936 1106 8 -0.10449056155482594 1106 9 -0.17429647032387408 1106 10-11 -0.1479546451585847 1106 12-13 -0.3358666845495364 1106 14-15 -0.12482496516691555 1106 16-17 -0.11492742253495436 1106 18-19 -0.09457189791108789 1106 20-21 -0.3161260292269077 1106 22-23 -0.2653563040093587 1106 24-25 -0.1826352274843046 1106 26-27 0.010303488371583569 1106 28-29 -0.17954245638642163 1106 30-31 -0.07684151838525821 1106 32-33 -0.08870500218484523 1106 34-35 -0.09041308569480577 1106 36-37 -0.10451622432685781 1106 38-39 -0.01586676355368155 1106 40-41 -0.05075694931506547 1106 42-43 -0.2737978153176712 1106 44-45 0.04006883592070665 1106 46-47 -0.04518306480667178 1106 48-49 -0.17911343426180082 1106 50-51 0.08109079060837132 1106 52-53 0.1407035113770405 1106 54-55 0.12665405300229793 1106 56-57 -0.020261103493162125 1106 58-59 -0.08615182777054287 1106 60-61 0.09563896941327954 1106 62-63 0.24172823426241763 1106 64-65 0.005172294861985449 1106 66-67 0.2735773996778619 1106 68-69 0.29430842180959615 1106 70-71 0.25301634991998867 1106 72-73 0.10746177239773402 1106 74-75 0.2395208914232363 1106 76-77 0.05354196486636553 1106 78-79 0.021838762248485466 1106 80-81 0.1317255358118814 1106 82-83 0.14282011799105376 1106 84-85 0.11840311343102528 1106 86-87 0.21804230151819226 1106 88-89 0.1956381138080765 1106 90-91 0.2436001371975287 1106 92-93 0.16665805091026797 1106 94-95 0.09417968583673897 1106 96-97 0.20918704306305713 1106 98-99 0.14687552529084869 1106 100-101 0.05546613516268195 1107 1 -0.11272692324062916 1107 2 -0.06901785263912075 1107 3 -0.033559700071251086 1107 4 -0.12661282150337883 1107 5 -0.1935338082482545 1107 6 -0.08310973891125428 1107 7 -0.13189293441053707 1107 8 -0.14949555172244544 1107 9 -0.22471330400199463 1107 10-11 -0.10391159610788492 1107 12-13 -0.13152806334226597 1107 14-15 -0.10271982282832681 1107 16-17 -0.10592102184914864 1107 18-19 -0.1326983701898783 1107 20-21 -0.1271858637129597 1107 22-23 -0.1691098471180439 1107 24-25 -0.15711621418346766 1107 26-27 -0.10850722421359649 1107 28-29 -0.2444858114828108 1107 30-31 -0.25025320586895816 1107 32-33 -0.1991244542438153 1107 34-35 -0.186254792018282 1107 36-37 -0.21376794129423615 1107 38-39 -0.15421288391132748 1107 40-41 -0.21738528180998173 1107 42-43 -0.15185838420574527 1107 44-45 -0.001297098047672307 1107 46-47 0.059199651906943984 1107 48-49 -0.08171280495368904 1107 50-51 -0.08682016740517895 1107 52-53 -0.04774223299187241 1107 54-55 -0.14279684025116524 1107 56-57 -0.17351876830992552 1107 58-59 -0.045136393670794916 1107 60-61 -0.1859786519311868 1107 62-63 -0.09166047065233585 1107 64-65 -0.059105480375116315 1107 66-67 -0.03868906451263854 1107 68-69 -0.10662397285214098 1107 70-71 -0.17760619702928437 1107 72-73 -0.11764365778341102 1107 74-75 0.01787797849246786 1107 76-77 0.027457690664501655 1107 78-79 -0.06845670678692173 1107 80-81 -0.0791402067098197 1107 82-83 -0.10643124477754284 1107 84-85 -0.19603091278685625 1107 86-87 -0.10534947909735948 1107 88-89 0.022255793438418436 1107 90-91 -0.034340878393496865 1107 92-93 0.04062919312511326 1107 94-95 -0.14270453510080117 1107 96-97 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42-43 -0.1932329905767176 2316 44-45 -0.030218895742024188 2316 46-47 -0.13153338531137848 2316 48-49 -0.19365841228654546 2316 50-51 -0.05260776269109613 2316 52-53 -0.12802750062749624 2316 54-55 -0.259469207613634 2316 56-57 -0.03889944632339137 2316 58-59 -0.013894774095092544 2316 60-61 0.021114378606874595 2316 62-63 0.21871913145515265 2316 64-65 0.17563050514672085 2316 66-67 0.10482213855899403 2316 68-69 -0.047859032613096986 2316 70-71 0.23832379521456204 2316 72-73 0.19972250834610605 2316 74-75 0.24324153836021267 2316 76-77 0.11770291693394697 2316 78-79 0.19519666039730765 2316 80-81 0.020660509811438743 2316 82-83 0.07887328478658517 2316 84-85 -0.1688825533828755 2316 86-87 -0.02283672479413923 2316 88-89 -0.3387435786821271 2316 90-91 -0.28498381250147986 2316 92-93 -0.33947995512670204 2316 94-95 -0.3327269926306151 2316 96-97 -0.3063544347612499 2316 98-99 -0.3902701171759624 2316 100-101 -0.1798425348346342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 22.0 5 44.0 6 88.0 7 241.0 8 575.0 9 1142.0 10 1881.0 11 3071.0 12 4722.0 13 6350.0 14 8404.0 15 11317.0 16 14902.0 17 19765.0 18 17803.0 19 11961.0 20 10735.0 21 11092.0 22 11705.0 23 13232.0 24 15314.0 25 18081.0 26 22736.0 27 28792.0 28 35285.0 29 44329.0 30 56429.0 31 71104.0 32 90935.0 33 119777.0 34 163365.0 35 232582.0 36 353313.0 37 500002.0 38 348447.0 39 26569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.88671184268303 20.217080472471334 15.531881881287907 22.364325803557733 2 15.990355525758284 20.975589181950703 39.17338350053293 23.860671791758087 3 19.494122019821432 24.415694765043348 30.154359036993622 25.93582417814159 4 13.474860727905384 17.33177512609177 36.922042774546156 32.27132137145669 5 13.629013832012232 37.68485157389669 34.396189605778865 14.289944988312214 6 32.165279365517314 37.04493871593878 16.77590217044605 14.01387974809786 7 28.45330786311103 32.9083414685153 20.564714896653165 18.073635771720497 8 26.46602422892973 35.595455779611115 19.326336637491313 18.612183353967847 9 26.105586607396265 16.684386865027445 20.19839006872215 37.01163645885414 10-11 24.829622489479913 27.149818858567336 27.153289674279325 20.86726897767343 12-13 25.6806776668832 24.13622582393429 27.95617546772596 22.226921041456546 14-15 22.54261854153688 25.733154901187195 26.110990535656704 25.61323602161923 16-17 21.9313156324661 29.000005272125133 26.90319323832378 22.16548585708499 18-19 21.918772368089233 27.439653937706325 29.397215966455224 21.244357727749215 20-21 22.489699585523095 26.521095969372467 29.780345693244914 21.208858751859523 22-23 22.249200767017626 26.33983696152412 29.623254983275032 21.787707288183213 24-25 22.255168303633898 26.51889111661807 29.592732687069756 21.633207892678275 26-27 21.838164383862573 26.94034686906187 29.50126863130609 21.72022011576947 28-29 21.91021306602872 27.12364156402196 29.478000311943255 21.488145058006065 30-31 22.060996042130864 26.771051428573937 29.450724562227865 21.71722796706733 32-33 21.98513524318995 26.72477573697719 29.30635967667814 21.983729343154717 34-35 22.29605882285747 26.87966637992164 29.230177468518825 21.594097328702063 36-37 22.423425374750728 26.66247238164362 29.21638776282197 21.697714480783688 38-39 22.152886149714952 26.94217156273505 29.205438923919385 21.699503363630612 40-41 22.3683236454309 26.82612805429238 29.07550369147855 21.73004460879817 42-43 22.146574773654503 26.824923599470086 28.98652582458746 22.041975802287954 44-45 22.237538233890803 27.011293770720552 29.013053781827157 21.738114213561488 46-47 22.630838768747896 27.201551586426387 28.355795515354625 21.81181412947109 48-49 22.476875685239122 27.341510547657492 28.18522649164379 21.996387275459593 50-51 22.571708056885363 27.54490478649756 28.127628511922588 21.755758644694495 52-53 22.733707127662097 27.40007328266812 27.966498221972675 21.899721367697104 54-55 22.589079144263795 27.54048333085836 27.944328115979722 21.926109408898117 56-57 22.562784420167283 27.741087692136013 27.828341363072806 21.8677865246239 58-59 22.49945301701754 27.473834882312985 27.891738667786704 22.13497343288277 60-61 22.355216460277614 27.550082992036458 28.178608176538866 21.916092371147062 62-63 22.3506472851631 27.569523953461193 28.294111651308672 21.785717110067036 64-65 22.205971384662156 27.573258375429788 28.14934788205553 22.071422357852523 66-67 22.26128476417345 27.549797418591798 27.975192015190746 22.213725802044003 68-69 22.354843018080754 27.429966407776035 28.131444623794334 22.08374595034888 70-71 22.346605322561796 27.436073286054086 27.889388178665293 22.327933212718825 72-73 22.36031284790534 27.421113630991663 27.829505624039484 22.389067897063512 74-75 22.29673980568704 27.725710660500624 27.651395663325605 22.32615387048673 76-77 22.45914322694478 27.47348340730418 27.840928561825773 22.226444803925272 78-79 22.3826315109647 27.560209865727757 27.76334045363122 22.293818169676317 80-81 22.38221413439174 27.62771503460711 27.678722845260488 22.31134798574066 82-83 22.359129752268178 27.643605176462216 27.652370128778898 22.344894942490708 84-85 22.35927315798966 27.638497170953823 27.630303404411144 22.371926266645374 86-87 22.38612429386474 27.608867187259733 27.57255542541217 22.432453093463355 88-89 22.29438931656563 27.54265808247537 27.57284099885683 22.590111602102173 90-91 22.362045958537223 27.486147366275016 27.530656819810527 22.62114985537723 92-93 22.228590433828252 27.40542656570867 27.71796198873317 22.64802101172991 94-95 22.189056649863232 27.437676890781777 27.584659346028022 22.788607113326968 96-97 22.24061364021788 27.39916841012916 27.5040397658825 22.856178183770464 98-99 21.97855040715827 27.50748490661908 27.4566519066439 23.057312779578755 100-101 22.034278498076965 27.522146874831105 27.49147673221494 22.95209789487699 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 282.0 1 218.0 2 205.0 3 311.0 4 305.5 5 304.0 6 551.0 7 1161.5 8 1901.0 9 2353.5 10 2277.0 11 2068.0 12 2146.0 13 2360.0 14 2596.0 15 2789.5 16 2946.0 17 3079.5 18 3369.5 19 3693.5 20 4069.5 21 4739.5 22 5688.5 23 6956.5 24 8664.5 25 11020.5 26 14386.5 27 18573.5 28 22080.5 29 25411.5 30 29445.5 31 33996.0 32 39546.5 33 43389.0 34 46309.0 35 50360.0 36 55543.0 37 60365.0 38 63447.0 39 65728.5 40 68337.0 41 71879.0 42 73532.0 43 75435.5 44 79148.5 45 81971.5 46 82743.5 47 84027.0 48 85795.5 49 84581.5 50 81990.5 51 79081.0 52 77340.0 53 76910.5 54 75755.5 55 70236.5 56 63262.0 57 57997.0 58 53082.5 59 46786.0 60 37522.5 61 30328.5 62 24952.5 63 18462.5 64 12952.0 65 9454.5 66 7029.0 67 5457.5 68 4320.0 69 3365.5 70 2436.5 71 1607.5 72 1104.0 73 865.0 74 640.0 75 423.0 76 300.0 77 181.0 78 111.5 79 84.5 80 58.0 81 30.0 82 20.5 83 12.0 84 5.5 85 3.5 86 3.0 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044856997999228515 2 0.0 3 0.0 4 8.78687522022106E-5 5 0.010632119016467483 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005997042337800873 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014718015993870277 24-25 5.491797012638162E-4 26-27 0.0020649156767519493 28-29 0.0029436031987740553 30-31 0.0010324578383759746 32-33 0.0 34-35 0.0 36-37 3.954093849099477E-4 38-39 4.3934376101105303E-4 40-41 6.590156415165796E-5 42-43 0.012609165941017221 44-45 0.0 46-47 0.0 48-49 2.196718805055265E-5 50-51 2.196718805055265E-5 52-53 1.757375044044212E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.05245325162711E-4 84-85 6.590156415165796E-5 86-87 0.0 88-89 0.0 90-91 0.008830809596322165 92-93 0.0026360625660663177 94-95 0.0 96-97 0.0 98-99 0.0018232766081958702 100-101 0.012872772197623854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.40330778124195 #Duplication Level Percentage of deduplicated Percentage of total 1 73.82013518672265 39.42239399829439 2 14.36335290605569 15.34101112025376 3 4.992755143247459 7.998889187736685 4 2.2357288653307497 4.775812668426595 5 1.258301600827482 3.3598733835309744 6 0.7463448664979178 2.3914370769922946 7 0.4952075781888261 1.8512005899534925 8 0.35478971852856334 1.5157555628960853 9 0.2616444385281789 1.2575410631973518 >10 1.3404340995093693 12.951515218625312 >50 0.08844706151530426 3.1933034270541896 >100 0.03981796225223904 3.8219442889236053 >500 0.0023009740074428787 0.8351864669723692 >1k 5.752435018606612E-4 0.6002669589221352 >5k 1.643552862459032E-4 0.6838689882207657 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8662 0.3805595657877741 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6921 0.3040698169957498 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4978 0.2187053242313022 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.39343761011053E-5 0.0 14-15 0.0 0.0 0.0 4.39343761011053E-5 0.0 16-17 0.0 0.0 0.0 4.39343761011053E-5 0.0 18-19 0.0 0.0 0.0 4.39343761011053E-5 0.0 20-21 0.0 0.0 0.0 4.39343761011053E-5 0.0 22-23 0.0 0.0 0.0 1.0983594025276326E-4 0.0 24-25 0.0 0.0 0.0 2.1967188050552651E-4 4.39343761011053E-5 26-27 0.0 0.0 0.0 3.295078207582897E-4 4.39343761011053E-5 28-29 0.0 0.0 0.0 7.249172056682375E-4 4.39343761011053E-5 30-31 0.0 0.0 0.0 0.0019770469245497385 4.39343761011053E-5 32-33 0.0 0.0 0.0 0.00472294543086882 4.39343761011053E-5 34-35 0.0 0.0 0.0 0.007490811125238453 4.39343761011053E-5 36-37 0.0 0.0 0.0 0.01177441279509622 4.39343761011053E-5 38-39 0.0 0.0 0.0 0.016058014464953987 6.590156415165794E-5 40-41 0.0 0.0 0.0 0.02286784276062531 8.78687522022106E-5 42-43 0.0 0.0 0.0 0.032621274255070686 8.78687522022106E-5 44-45 0.0 0.0 0.0 0.0476468308816487 1.0983594025276326E-4 46-47 0.0 0.0 0.0 0.07451270186747459 1.5377031635386857E-4 48-49 0.0 0.0 0.0 0.11271364188738564 1.757375044044212E-4 50-51 0.0 0.0 0.0 0.15065097565069008 1.757375044044212E-4 52-53 0.0 0.0 0.0 0.19010404538948264 2.4163906855607917E-4 54-55 0.0 0.0 0.0 0.23823415440824353 2.6360625660663183E-4 56-57 0.0 0.0 0.0 0.2923173713887041 2.6360625660663183E-4 58-59 0.0 0.0 0.0 0.357977296471806 2.6360625660663183E-4 60-61 0.0 0.0 0.0 0.44283654391109084 2.6360625660663183E-4 62-63 0.0 0.0 0.0 0.5290138226334089 3.075406327077371E-4 64-65 0.0 0.0 0.0 0.6289205938873224 3.295078207582897E-4 66-67 0.0 0.0 0.0 0.7623712612944298 3.514750088088424E-4 68-69 0.0 0.0 0.0 0.91541666044263 3.514750088088424E-4 70-71 0.0 0.0 0.0 1.082169584934375 3.734421968593951E-4 72-73 0.0 0.0 0.0 1.260191676896054 4.6131094906160566E-4 74-75 0.0 0.0 0.0 1.441442945501164 5.05245325162711E-4 76-77 0.0 0.0 0.0 1.6406414067435753 5.272125132132637E-4 78-79 0.0 0.0 0.0 1.8758440892008426 5.272125132132637E-4 80-81 0.0 0.0 0.0 2.1239634782318344 5.272125132132637E-4 82-83 0.0 0.0 0.0 2.413754622994725 5.711468893143689E-4 84-85 0.0 0.0 0.0 2.7317955715906264 5.711468893143689E-4 86-87 0.0 0.0 0.0 3.115408576517427 5.711468893143689E-4 88-89 0.0 0.0 0.0 3.579311653768998 5.711468893143689E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4680 0.0 49.241104 1 GTATCAA 8250 0.0 41.352562 1 ATCAACG 10470 0.0 32.301456 3 TCAACGC 10525 0.0 32.087532 4 TATCAAC 10715 0.0 31.651539 2 CAACGCA 10825 0.0 31.242147 5 AACGCAG 11145 0.0 30.386395 6 TACCGTG 210 4.3655746E-11 27.145538 7 TAAGGTG 945 0.0 26.639915 5 ACGCAGA 12770 0.0 26.37496 7 CGCAGAG 12740 0.0 26.287916 8 GCAGAGT 14030 0.0 23.4984 9 GTAAGGT 935 0.0 22.86071 4 GAGTACT 8555 0.0 22.683418 12-13 GTACATG 7520 0.0 21.862015 1 TGGTATC 2310 0.0 21.796295 2 TACATGG 7465 0.0 21.506794 2 AGAGTAC 12410 0.0 21.302465 10-11 AGTACTT 8940 0.0 21.228327 12-13 GTACTTT 9095 0.0 21.17668 14-15 >>END_MODULE