FastQCFastQC Report
Thu 26 May 2016
SRR937161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430250
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87390.3595926345026232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62400.2567637074375064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.17936426293591196No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35670.14677502314576688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.044.2830621
GTATCAA78800.043.511811
ATCAACG99850.034.181823
TCAACGC102250.033.2872164
TATCAAC103900.032.849422
CAACGCA103800.032.8345955
AACGCAG106500.031.9790676
GTATCGA1452.3754183E-829.5170271
CGCAGAG122400.027.6314568
ACGCAGA123250.027.5174067
TGTACGC1754.9276423E-927.1631892
GTACATG78300.025.751481
GTGTACG2800.025.4760041
TACATGG78100.024.9546592
GCAGAGT134600.024.597619
TAGCGCG1000.001646781623.768284
GTATAGG6850.022.909811
ACATGGG79550.022.7071293
AGTACTT83000.022.28987912-13
GAGTACT79550.022.21166412-13
Produced by FastQC (version 0.11.5)