##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937161_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2430250 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.831852690052465 33.0 31.0 34.0 30.0 34.0 2 31.95515564242362 33.0 31.0 34.0 30.0 34.0 3 32.00904968624627 34.0 31.0 34.0 30.0 34.0 4 35.62490772554264 37.0 35.0 37.0 35.0 37.0 5 35.61994156979735 37.0 35.0 37.0 33.0 37.0 6 35.67317395329699 37.0 35.0 37.0 35.0 37.0 7 35.6153519185269 37.0 35.0 37.0 33.0 37.0 8 35.60204917189589 37.0 35.0 37.0 33.0 37.0 9 37.248051023557245 39.0 37.0 39.0 34.0 39.0 10-11 37.16569941364057 39.0 37.0 39.0 34.0 39.0 12-13 37.05993642629359 39.0 37.0 39.0 33.0 39.0 14-15 38.2688612282687 40.0 38.0 41.0 33.5 41.0 16-17 38.21006645406851 40.0 38.0 41.0 33.0 41.0 18-19 38.15408332476083 40.0 38.0 41.0 33.5 41.0 20-21 38.08175372904022 40.0 38.0 41.0 33.5 41.0 22-23 37.94793910091554 40.0 38.0 41.0 33.0 41.0 24-25 37.80634996399547 40.0 38.0 41.0 33.0 41.0 26-27 37.633942187017794 40.0 37.0 41.0 32.5 41.0 28-29 37.4573539759284 40.0 37.0 41.0 32.0 41.0 30-31 37.453259335459315 40.0 37.0 41.0 32.0 41.0 32-33 37.30311058533073 40.0 37.0 41.0 32.0 41.0 34-35 37.1086558995988 39.0 36.0 41.0 31.0 41.0 36-37 37.14767431334225 40.0 37.0 41.0 31.0 41.0 38-39 37.12839543256867 40.0 37.0 41.0 31.0 41.0 40-41 36.97766546651579 40.0 36.5 41.0 31.0 41.0 42-43 36.763333196173235 39.5 36.0 41.0 31.0 41.0 44-45 36.56966752391729 39.0 36.0 41.0 30.0 41.0 46-47 36.28461063676576 39.0 36.0 41.0 30.0 41.0 48-49 35.999702911223125 39.0 35.5 41.0 29.0 41.0 50-51 35.0870305524123 38.0 34.0 40.0 27.0 40.5 52-53 35.21564633268182 38.5 35.0 40.0 28.0 40.5 54-55 35.56955765867709 39.0 35.0 40.0 28.0 41.0 56-57 35.35291369200699 39.0 35.0 40.0 27.5 41.0 58-59 35.11296080650139 38.0 35.0 40.0 26.5 41.0 60-61 34.83615389363234 38.0 34.0 40.0 26.0 41.0 62-63 34.51506593971814 38.0 34.0 40.0 26.0 41.0 64-65 34.16013146795597 37.0 34.0 40.0 25.5 41.0 66-67 33.7758893117992 36.5 33.5 39.5 24.5 41.0 68-69 33.285109556629976 36.0 33.0 39.0 22.5 41.0 70-71 32.86405102355725 35.5 32.5 39.0 22.0 40.0 72-73 32.46931797140212 35.0 32.0 38.0 21.5 40.0 74-75 32.01947186503446 35.0 32.0 37.0 20.0 39.5 76-77 31.58874745396564 35.0 31.0 37.0 18.5 39.0 78-79 31.126646847032198 35.0 31.0 36.0 17.5 39.0 80-81 30.699895895484005 34.5 31.0 36.0 13.5 37.5 82-83 30.26679765456229 34.0 30.0 35.0 7.5 37.0 84-85 29.82017323320646 34.0 30.0 35.0 6.5 37.0 86-87 29.40381730274663 34.0 29.5 35.0 2.0 36.0 88-89 29.029784178582453 34.0 29.0 35.0 2.0 36.0 90-91 28.61572266227754 34.0 29.0 35.0 2.0 35.5 92-93 28.18938401399033 34.0 28.5 35.0 2.0 35.0 94-95 27.668828104104517 33.5 27.0 35.0 2.0 35.0 96-97 27.06478098961012 33.0 26.0 35.0 2.0 35.0 98-99 26.371953091245757 33.0 25.0 35.0 2.0 35.0 100-101 24.553544697047627 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0444647456332774 1101 2 -0.006397089482547358 1101 3 0.10102665704055624 1101 4 0.054294551440172256 1101 5 0.07256976271644078 1101 6 -0.12068870966671597 1101 7 -0.020892918971675556 1101 8 -0.057035592213360076 1101 9 -0.03822855477245923 1101 10-11 -0.009106531763329428 1101 12-13 0.030910796899064508 1101 14-15 0.0591248967710456 1101 16-17 0.05207779314985572 1101 18-19 0.12760597358774106 1101 20-21 0.06316219677751889 1101 22-23 0.08971288079735729 1101 24-25 0.1409144622594951 1101 26-27 0.08971559062234036 1101 28-29 0.08611251350660609 1101 30-31 0.03260612200512014 1101 32-33 -0.07929578755972244 1101 34-35 -0.14072113575848988 1101 36-37 -0.05707773911120029 1101 38-39 0.14660226462689963 1101 40-41 -0.002206770523358159 1101 42-43 0.13121796459143553 1101 44-45 0.0824697779234782 1101 46-47 0.07066054217941797 1101 48-49 0.18328155627621356 1101 50-51 0.19883593426826707 1101 52-53 0.12316426714196638 1101 54-55 0.3052154987491633 1101 56-57 0.3547131068305518 1101 58-59 0.32620404199268904 1101 60-61 0.2841155034868237 1101 62-63 0.3745385358018609 1101 64-65 0.3284295217866813 1101 66-67 0.30390612806676387 1101 68-69 0.1513026980232084 1101 70-71 0.22405218753913658 1101 72-73 0.21948203834194402 1101 74-75 0.11309390271976838 1101 76-77 0.11983909050929142 1101 78-79 0.25893527567391317 1101 80-81 0.2994705698221587 1101 82-83 0.18243991623844025 1101 84-85 0.2423079405086206 1101 86-87 0.49105434377186796 1101 88-89 0.3586066107479553 1101 90-91 0.4368423046010932 1101 92-93 0.26336354460135425 1101 94-95 0.31351849117427477 1101 96-97 0.34052872267678325 1101 98-99 0.41151798022828245 1101 100-101 0.6699566534096562 1102 1 -0.05979913681912663 1102 2 -0.29751815566055484 1102 3 -0.16541645871061306 1102 4 -0.23862393419280892 1102 5 -0.10431440743973752 1102 6 -0.06685195583629167 1102 7 -0.15627533194113852 1102 8 -0.011645118393424525 1102 9 -0.11041974713102576 1102 10-11 -0.17527444671743098 1102 12-13 0.0032580832999329346 1102 14-15 0.03120655776400838 1102 16-17 0.03807206543994823 1102 18-19 0.06641829810600086 1102 20-21 0.06130733392820531 1102 22-23 -0.07141762844883459 1102 24-25 0.029235518352493273 1102 26-27 -0.015746100489728576 1102 28-29 -0.007557456892840264 1102 30-31 -0.056114418250672315 1102 32-33 -0.10819607333726111 1102 34-35 -0.09266367913205187 1102 36-37 0.009850565453056959 1102 38-39 0.11494384656344181 1102 40-41 0.0999730475961016 1102 42-43 -0.032038710196879094 1102 44-45 0.13007023546794017 1102 46-47 0.027726589297849102 1102 48-49 0.05624157817712927 1102 50-51 0.01758901654223166 1102 52-53 0.09899056690046137 1102 54-55 0.18390407991430635 1102 56-57 0.025546811199042452 1102 58-59 -0.1258561150544253 1102 60-61 -0.030133122610237706 1102 62-63 -0.05248443726438268 1102 64-65 -0.1395003899409204 1102 66-67 0.011469406994720543 1102 68-69 0.07394526582250904 1102 70-71 -0.01571604870457577 1102 72-73 -0.13911762670718275 1102 74-75 -0.12231502808745276 1102 76-77 -0.16428416325622308 1102 78-79 -0.050984398344436244 1102 80-81 -0.02971910549436174 1102 82-83 0.028561356133788962 1102 84-85 -0.04100766360277319 1102 86-87 -0.1527021985166641 1102 88-89 0.06516492084465497 1102 90-91 0.03310263681255776 1102 92-93 -0.18056674048596477 1102 94-95 -0.3347074601151583 1102 96-97 -0.10714486008515678 1102 98-99 -0.08185412493756061 1102 100-101 -0.09815951796531408 1103 1 -0.041869692509379774 1103 2 -0.13902376393313887 1103 3 -0.03540867002692849 1103 4 -0.08908675228327212 1103 5 -0.1455674223239143 1103 6 -0.02442066666650078 1103 7 0.01576702297261079 1103 8 0.008084335521388653 1103 9 0.06001335787794204 1103 10-11 0.019413848086706764 1103 12-13 0.01713703647723719 1103 14-15 0.037555332139639575 1103 16-17 0.06490130384906223 1103 18-19 0.006199002257027075 1103 20-21 0.0038641596152544366 1103 22-23 0.07992635994209962 1103 24-25 -0.0830759320499368 1103 26-27 -0.05232112052039639 1103 28-29 -0.2311453991269019 1103 30-31 -0.23067850371710108 1103 32-33 -0.16125615674030058 1103 34-35 -0.018219549589673534 1103 36-37 0.04112889601859848 1103 38-39 0.09149851352677985 1103 40-41 0.08884217883994694 1103 42-43 0.06086645093068199 1103 44-45 0.02806396749590334 1103 46-47 0.045386617950264 1103 48-49 -0.07432418039407196 1103 50-51 -0.18213920678022788 1103 52-53 -0.09449266855934724 1103 54-55 0.054047029849854766 1103 56-57 0.21815074549936497 1103 58-59 0.08039630465590619 1103 60-61 0.03228107241557865 1103 62-63 0.1288712927304161 1103 64-65 0.0842714704008003 1103 66-67 0.18615033613129128 1103 68-69 0.17129625481720723 1103 70-71 0.24498306189732233 1103 72-73 0.10650247959774362 1103 74-75 0.10659272193452551 1103 76-77 0.051952912505786486 1103 78-79 0.3407615286944967 1103 80-81 0.16359277713574372 1103 82-83 0.04624933246849494 1103 84-85 0.08405029989031121 1103 86-87 0.1270197144427172 1103 88-89 0.23589230376912695 1103 90-91 0.41947921041239766 1103 92-93 0.23054071481987748 1103 94-95 0.11740098905498897 1103 96-97 0.17791210891759945 1103 98-99 0.33191918152902744 1103 100-101 0.7116500201449085 1104 1 -0.01847048382347083 1104 2 -0.03644180659584251 1104 3 -0.04800255654980745 1104 4 -0.09331475284221824 1104 5 -0.02817385989020238 1104 6 -0.054338445853097994 1104 7 -0.06529883412193271 1104 8 -0.012021919012617843 1104 9 0.04331132304702834 1104 10-11 -0.023183910585807155 1104 12-13 -0.004836056963590352 1104 14-15 0.10361294871403004 1104 16-17 0.0472103071754546 1104 18-19 0.021524001610764287 1104 20-21 0.04078818865384193 1104 22-23 0.10841106413752044 1104 24-25 0.12417292370453481 1104 26-27 0.21691918794278564 1104 28-29 0.09574745748008695 1104 30-31 0.15526834081536833 1104 32-33 0.09500146847502577 1104 34-35 0.06776644125491771 1104 36-37 -0.0248074673170251 1104 38-39 0.04196270147535586 1104 40-41 0.05274165949825971 1104 42-43 0.06846789465318182 1104 44-45 0.15752193343146814 1104 46-47 0.1934954380093714 1104 48-49 0.34720463720291406 1104 50-51 0.28687735940397374 1104 52-53 0.3555110915112678 1104 54-55 0.40820094074811664 1104 56-57 0.3790413200327336 1104 58-59 0.21282517831048864 1104 60-61 0.36453962210211444 1104 62-63 0.320460267841824 1104 64-65 0.41599466195597046 1104 66-67 0.40737056581737363 1104 68-69 0.38647795571045407 1104 70-71 0.3449290831912535 1104 72-73 0.321441719206625 1104 74-75 0.48485099033885604 1104 76-77 0.36771003978291006 1104 78-79 0.33107631891418876 1104 80-81 0.5109381130483435 1104 82-83 0.5167296146039497 1104 84-85 0.500447615344509 1104 86-87 0.5079750652310935 1104 88-89 0.4843550585523282 1104 90-91 0.6087529853117957 1104 92-93 0.34071932540508953 1104 94-95 0.5430770018471804 1104 96-97 0.6830776415072002 1104 98-99 0.7052475544615113 1104 100-101 0.8158587051487878 1105 1 0.039000710523488635 1105 2 0.1674260489493662 1105 3 0.09715665404036855 1105 4 0.0604468106910403 1105 5 0.13811216989348907 1105 6 0.0341801893123872 1105 7 0.0782161957046057 1105 8 0.15477765074282246 1105 9 0.18448516698814643 1105 10-11 0.15059236061852488 1105 12-13 0.1658559792680876 1105 14-15 0.1741592585871743 1105 16-17 0.2857026663139166 1105 18-19 0.2633820375569371 1105 20-21 0.23092077902946784 1105 22-23 0.20586608674729945 1105 24-25 0.29334007720871824 1105 26-27 0.20503233162168755 1105 28-29 0.15667128928834018 1105 30-31 0.10290015803818875 1105 32-33 0.09814570782160814 1105 34-35 0.2651456512318475 1105 36-37 0.3537001233885704 1105 38-39 0.374689442368755 1105 40-41 0.2995423399945665 1105 42-43 0.3629782457630384 1105 44-45 0.37282727243994174 1105 46-47 0.3515316278554863 1105 48-49 0.3857820177673048 1105 50-51 0.3104216136958087 1105 52-53 0.31886979116064396 1105 54-55 0.4764633785583072 1105 56-57 0.5093321879355912 1105 58-59 0.5478853380256794 1105 60-61 0.6253468565787017 1105 62-63 0.6361091648883317 1105 64-65 0.5636612634402169 1105 66-67 0.5802402227121632 1105 68-69 0.7007068507710343 1105 70-71 0.6659081149622992 1105 72-73 0.5330880605338351 1105 74-75 0.4610968222685301 1105 76-77 0.5484503719208256 1105 78-79 0.7793507718133945 1105 80-81 0.7697009853064429 1105 82-83 0.6910944058648738 1105 84-85 0.7158187967159044 1105 86-87 0.6292973098373515 1105 88-89 0.8645900848212484 1105 90-91 0.8200487427081242 1105 92-93 0.7716685560190584 1105 94-95 0.8768010743227173 1105 96-97 0.9215002493683606 1105 98-99 0.9336701948123505 1105 100-101 1.2484084585216735 1106 1 -0.05030737833376975 1106 2 -0.09173480855804783 1106 3 -0.0865195610929348 1106 4 4.84494532955182E-5 1106 5 0.028405149156810694 1106 6 -0.018611820034074356 1106 7 0.04271706459933711 1106 8 -0.057342267980175166 1106 9 0.007566880207534155 1106 10-11 -0.12798643542729593 1106 12-13 -0.19176300908862487 1106 14-15 -0.22308540043297143 1106 16-17 -0.22505439308604736 1106 18-19 -0.16588072240252671 1106 20-21 -0.22275773142876432 1106 22-23 -0.0901306763690215 1106 24-25 -0.18652653646095274 1106 26-27 -0.03439540061236812 1106 28-29 0.06601668326480592 1106 30-31 0.01930846050332491 1106 32-33 0.08171575112890395 1106 34-35 0.019728095208932928 1106 36-37 -0.27697716680116 1106 38-39 -0.2789521155618715 1106 40-41 -0.038552700375433346 1106 42-43 -0.03962134019699448 1106 44-45 -0.03277264767022814 1106 46-47 0.12095609049834621 1106 48-49 0.032714128690301436 1106 50-51 -0.05554382096090649 1106 52-53 0.04552987998357594 1106 54-55 0.0691328600039256 1106 56-57 -0.13449966855313988 1106 58-59 -0.23864921194970634 1106 60-61 -0.10954955166113933 1106 62-63 0.07746442493449734 1106 64-65 -0.1316605651230205 1106 66-67 -0.10470248057284692 1106 68-69 0.03809061877596065 1106 70-71 -0.09636871282209825 1106 72-73 0.006919500639718024 1106 74-75 0.047195979609398364 1106 76-77 -0.08789105876366321 1106 78-79 -0.17412662225167352 1106 80-81 0.048869966338752846 1106 82-83 -0.057772574662440235 1106 84-85 -0.002650902385443743 1106 86-87 -0.0029265120630661556 1106 88-89 0.23389568697984942 1106 90-91 0.33243427657065894 1106 92-93 0.21072385177672004 1106 94-95 0.14159446666333864 1106 96-97 0.25399134498064413 1106 98-99 0.30154664114831675 1106 100-101 0.5227660140087451 1107 1 -0.11344488887905868 1107 2 -0.14906134964484608 1107 3 -0.10684173067885183 1107 4 -0.11059004925177618 1107 5 -0.10066916635818046 1107 6 -0.07930990329906962 1107 7 -0.21596399282012158 1107 8 -0.04469529764856617 1107 9 -0.01582300929555913 1107 10-11 -0.10974898800625255 1107 12-13 -0.09152378592969512 1107 14-15 -0.05630688981099041 1107 16-17 -0.13816712589354552 1107 18-19 -0.01252496128352476 1107 20-21 0.015189668973583537 1107 22-23 0.018634005915480145 1107 24-25 -0.0529487196156353 1107 26-27 0.008571851477519488 1107 28-29 -0.03720324275595033 1107 30-31 -0.11565720463145368 1107 32-33 -0.0263867166157965 1107 34-35 -0.009474238617990238 1107 36-37 -0.06503459215822005 1107 38-39 0.06888307708504016 1107 40-41 0.10649809398704946 1107 42-43 0.10266334421131518 1107 44-45 0.1581855428469794 1107 46-47 0.11146459457193458 1107 48-49 0.15484699046002248 1107 50-51 0.049478390511190184 1107 52-53 0.0032668760958287635 1107 54-55 0.1877356315708809 1107 56-57 0.1000120330285128 1107 58-59 0.12156378810338708 1107 60-61 0.014103896279067385 1107 62-63 0.2093299231145096 1107 64-65 0.18476442027581896 1107 66-67 0.1628528416262327 1107 68-69 0.13177111574659506 1107 70-71 0.1818830702901053 1107 72-73 0.19319646855255002 1107 74-75 0.09539310421192226 1107 76-77 0.0940343189493511 1107 78-79 0.16546554798318525 1107 80-81 0.26430502211816176 1107 82-83 0.23608033406699036 1107 84-85 0.27835814148885163 1107 86-87 0.2578849883469445 1107 88-89 0.24333335032118697 1107 90-91 0.2993191249839988 1107 92-93 0.29846432857119254 1107 94-95 0.11189584064789315 1107 96-97 0.15493422639557153 1107 98-99 0.1763239364812499 1107 100-101 0.36835793646622506 1108 1 -0.1908827076907329 1108 2 -0.23031805622131074 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-1.1700225136787594 2316 100-101 -1.3206086094225427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9452.0 3 1660.0 4 1488.0 5 1663.0 6 1914.0 7 2263.0 8 2703.0 9 3311.0 10 4176.0 11 5358.0 12 7220.0 13 9023.0 14 10845.0 15 13197.0 16 16108.0 17 20665.0 18 17746.0 19 13092.0 20 12536.0 21 13490.0 22 14443.0 23 15908.0 24 18223.0 25 20651.0 26 24252.0 27 28631.0 28 34713.0 29 42274.0 30 52551.0 31 67101.0 32 87393.0 33 116373.0 34 163403.0 35 236144.0 36 355319.0 37 506197.0 38 417209.0 39 61538.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93046274918399 19.021431546566152 14.903628836342605 23.14447686790725 2 15.114921136922414 19.74968988748466 40.74065515240699 24.394733823185934 3 18.968093583681043 24.04179027856472 29.822597238494037 27.167518899260205 4 12.591154350091305 16.350730947394485 37.69191882718278 33.36619587533143 5 13.602311016776412 37.14383446900833 34.76444612427698 14.48940838993828 6 32.08623559042754 36.25286789459013 17.45160247476005 14.209294040222273 7 28.452226554526206 32.450354339394664 20.91453997736022 18.182879128718916 8 26.17858023566244 34.95225142825096 19.89971933951177 18.969448996574837 9 25.817412427976038 16.683951455241743 20.51688596699217 36.98175014979005 10-11 24.546625740811994 26.750679313926955 27.585753975569027 21.11694096969202 12-13 25.42199117232119 23.797040092408963 28.247953604168135 22.53301513110171 14-15 22.514928580926668 25.295206052041035 26.34635544711525 25.84350991991704 16-17 21.915639878243123 28.547525738696944 27.072245258910684 22.46458912414925 18-19 21.85836409599021 27.141548904068753 29.453294019805476 21.546792980135564 20-21 22.436591801332593 26.263514870326105 29.682162164836782 21.61773116350452 22-23 22.17754604717903 26.057631647307677 29.6044705038285 22.16035180168479 24-25 21.998003241897372 26.2723148437711 29.710837020134356 22.018844894197176 26-27 21.764692420406025 26.589967203907904 29.51532831244757 22.130012063238503 28-29 21.803383028693332 26.81986602066616 29.475309119241857 21.901441831398653 30-31 21.892631573304147 26.474626617622587 29.43382874340667 22.198913065666595 32-33 21.79870891595252 26.465841451150922 29.36301722799426 22.372432404902302 34-35 22.23816633864396 26.594538782757425 29.206143817854414 21.961151060744196 36-37 22.20109251403577 26.322038468896995 29.239821179994323 22.237047837072907 38-39 21.87423984432012 26.706954058767508 29.224136153931784 22.194669942980592 40-41 22.21172476675 26.543117364965056 29.10818194767952 22.13697592060543 42-43 21.901850532516665 26.46060712506968 29.106966749454948 22.53057559295871 44-45 22.049149265099146 26.699995587667075 29.066387466335343 22.184467680898436 46-47 22.304895328645355 26.90813416916758 28.510298701408477 22.27667180077859 48-49 22.187052525342075 26.94312632534971 28.43434254922801 22.435478600080206 50-51 22.272909473906466 27.17110700187885 28.31730237882759 22.238681145387094 52-53 22.374014453140706 27.122950676002404 28.16092084384566 22.342114027011228 54-55 22.200178944401245 27.151276478997747 28.227850372127232 22.420694204473776 56-57 22.16046685163732 27.37340297904445 28.154649758205153 22.31148041111308 58-59 22.22527950995456 27.016269236437235 28.249008871974183 22.509442381634017 60-61 22.127044263694042 27.175525314446613 28.349850970239395 22.34757945161995 62-63 22.0466356114382 27.192247447466762 28.520886011893165 22.24023092920187 64-65 21.966031124394515 27.149809337318352 28.4332680614243 22.450891476862825 66-67 21.93149778732192 27.215991889355166 28.207022642560393 22.645487680762514 68-69 22.075704230069753 27.016182641293817 28.429043542392524 22.4790695862439 70-71 22.057249623596423 26.986219423908757 28.292376941178798 22.66415401131602 72-73 22.026006497810457 27.08332259573488 28.14932388177275 22.741347024681907 74-75 21.97127660956057 27.32797515743567 28.05829443608768 22.642453796916076 76-77 22.151691134585892 27.126146350655322 28.099159241488664 22.623003273270122 78-79 22.093302049443857 27.140024817379505 28.12917406862156 22.63749906455508 80-81 22.137101362281893 27.192373464114155 27.91684325265621 22.753681920947745 82-83 22.106984468879208 27.23345416942231 28.021761216233376 22.6378001454651 84-85 22.151206399833782 27.27689842792983 27.908744118572788 22.6631510536636 86-87 22.13394127971752 27.221232225143545 27.953715546641572 22.691110948497364 88-89 22.144469237442156 27.069335734570892 27.941907697483646 22.844287330503306 90-91 22.114909697788764 27.077841388369183 27.959486658954237 22.84776225488781 92-93 22.090772111521424 26.9076844199562 28.143448772904385 22.85809469561799 94-95 22.02135291474089 26.97266051792878 28.065105981207456 22.940880586122873 96-97 22.086416045142627 26.870039926919866 27.967437575665627 23.07610645227188 98-99 21.893055763712614 27.043515350446352 27.894101782994905 23.169327102846125 100-101 22.146933879136032 26.714836640510185 28.06019494720266 23.078034533151122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3223.0 1 1837.0 2 491.0 3 515.0 4 414.5 5 374.0 6 654.5 7 1317.5 8 2215.0 9 2662.5 10 2419.5 11 2178.5 12 2210.5 13 2413.0 14 2620.5 15 2722.0 16 2833.0 17 3034.5 18 3348.0 19 3631.0 20 3968.5 21 4740.0 22 5872.0 23 7253.0 24 9048.0 25 11530.0 26 15197.0 27 19665.0 28 23354.5 29 26725.0 30 30703.0 31 35514.5 32 41641.0 33 45675.5 34 48891.5 35 53312.0 36 59012.5 37 64087.0 38 66622.0 39 69024.5 40 72174.0 41 75897.0 42 77940.0 43 80380.0 44 84325.5 45 87605.0 46 88766.5 47 89900.0 48 91409.0 49 90289.5 50 87792.5 51 84426.5 52 82572.0 53 82502.0 54 80829.0 55 74796.5 56 67600.0 57 61957.5 58 57094.5 59 50835.5 60 41375.0 61 33534.5 62 27350.5 63 20465.0 64 14546.5 65 10941.0 66 8218.5 67 6006.0 68 4633.0 69 3579.0 70 2703.5 71 1853.0 72 1264.0 73 968.0 74 709.5 75 500.5 76 354.0 77 241.0 78 174.0 79 114.0 80 76.5 81 53.0 82 36.0 83 24.5 84 13.0 85 12.5 86 13.0 87 12.0 88 9.0 89 5.0 90 7.0 91 6.0 92 5.0 93 5.0 94 2.0 95 2.0 96 4.0 97 2.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3206665980866166 2 0.28540273634399754 3 0.28832424647669996 4 0.289064911017385 5 0.27684394609608065 6 0.21092480197510544 7 0.21870177965229914 8 0.2162740458800535 9 0.21351712786750335 10-11 0.221252957514659 12-13 0.221252957514659 14-15 0.2037856187635017 16-17 0.21617117580495834 18-19 0.20413537701882523 20-21 0.2078386997222508 22-23 0.20656311079107087 24-25 0.1982717827384014 26-27 0.21041045159962968 28-29 0.21292048143195147 30-31 0.22040942289887874 32-33 0.2217261598600967 34-35 0.21038987758461064 36-37 0.20648081473099478 38-39 0.1958646229811748 40-41 0.1972842300174879 42-43 0.1969344717621644 44-45 0.21516304906902584 46-47 0.2044439872441107 48-49 0.21324966567225595 50-51 0.2204711449439358 52-53 0.22703425573500668 54-55 0.22526489044337 56-57 0.19092685937660733 58-59 0.17909680074066453 60-61 0.18763501697356239 62-63 0.1881082193190001 64-65 0.1851661351712787 66-67 0.19732537804752598 68-69 0.18654459417755373 70-71 0.1918321160374447 72-73 0.2045674313342249 74-75 0.19485649624524226 76-77 0.2057195761752906 78-79 0.2030038061927785 80-81 0.18985701059561771 82-83 0.20269519596749305 84-85 0.18514556115625966 86-87 0.18917806809998972 88-89 0.19890957720399136 90-91 0.1945478860199568 92-93 0.2003291842403045 94-95 0.1983335047834585 96-97 0.19312827898364365 98-99 0.20016459212015225 100-101 0.18372595411994652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2430250.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.51970405648648 #Duplication Level Percentage of deduplicated Percentage of total 1 74.26831514246554 39.7481824719863 2 13.998374451528875 14.983777158354137 3 4.926853476979991 7.910512200529215 4 2.2451604363184967 4.806412884443921 5 1.2287814533834487 3.288200986759076 6 0.7767280832106254 2.4942154287476805 7 0.4905784082343144 1.8378927857641905 8 0.33339009346838194 1.4274351310233735 9 0.25602468420515617 1.233212879683383 >10 1.341592158041374 13.031121169456492 >50 0.09076408599477478 3.327832019475596 >100 0.04043864788000607 3.8991637432146313 >500 0.0020761465077311367 0.6850841436455144 >1k 7.689431510100461E-4 0.7105170745491924 >5k 1.5378863020200923E-4 0.6164399223673533 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8739 0.3595926345026232 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6240 0.2567637074375064 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4359 0.17936426293591196 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3567 0.14677502314576688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.229606007612386E-5 7 0.0 0.0 0.0 0.0 8.229606007612386E-5 8 0.0 0.0 0.0 0.0 8.229606007612386E-5 9 0.0 0.0 0.0 0.0 1.2344409011418579E-4 10-11 0.0 0.0 0.0 0.0 1.2344409011418579E-4 12-13 0.0 0.0 0.0 0.0 1.2344409011418579E-4 14-15 0.0 0.0 0.0 0.0 1.6459212015224772E-4 16-17 0.0 0.0 0.0 0.0 1.6459212015224772E-4 18-19 0.0 0.0 0.0 0.0 1.6459212015224772E-4 20-21 0.0 0.0 0.0 0.0 1.6459212015224772E-4 22-23 0.0 0.0 0.0 8.229606007612386E-5 1.851661351712787E-4 24-25 0.0 0.0 0.0 1.4401810513321674E-4 2.0574015019030964E-4 26-27 0.0 0.0 0.0 2.263141652093406E-4 2.0574015019030964E-4 28-29 0.0 0.0 0.0 6.583684806089909E-4 2.0574015019030964E-4 30-31 0.0 0.0 0.0 0.0017899393066556938 2.0574015019030964E-4 32-33 0.0 0.0 0.0 0.003538730583273326 2.0574015019030964E-4 34-35 0.0 0.0 0.0 0.006460240715975722 2.0574015019030964E-4 36-37 0.0 0.0 0.0 0.010060693344306142 2.0574015019030964E-4 38-39 0.0 0.0 0.0 0.014401810513321674 2.0574015019030964E-4 40-41 0.0 0.0 0.0 0.021726159860096697 2.0574015019030964E-4 42-43 0.0 0.0 0.0 0.03308301615060179 2.0574015019030964E-4 44-45 0.0 0.0 0.0 0.04843123135479889 2.263141652093406E-4 46-47 0.0 0.0 0.0 0.07704968624627095 2.4688818022837157E-4 48-49 0.0 0.0 0.0 0.1159551486472585 2.4688818022837157E-4 50-51 0.0 0.0 0.0 0.1570003086102253 2.880362102664335E-4 52-53 0.0 0.0 0.0 0.1988067071288962 3.2918424030449543E-4 54-55 0.0 0.0 0.0 0.24727908651373315 3.2918424030449543E-4 56-57 0.0 0.0 0.0 0.3034255735006687 3.2918424030449543E-4 58-59 0.0 0.0 0.0 0.3735623907005452 3.2918424030449543E-4 60-61 0.0 0.0 0.0 0.4601995679456846 3.2918424030449543E-4 62-63 0.0 0.0 0.0 0.5483797963172513 3.7033227034255733E-4 64-65 0.0 0.0 0.0 0.6463944038679148 3.7033227034255733E-4 66-67 0.0 0.0 0.0 0.7798168912663306 3.7033227034255733E-4 68-69 0.0 0.0 0.0 0.9363645715461373 3.7033227034255733E-4 70-71 0.0 0.0 0.0 1.1080753008949698 3.7033227034255733E-4 72-73 0.0 0.0 0.0 1.279024791688098 3.7033227034255733E-4 74-75 0.0 0.0 0.0 1.4626067277029113 3.7033227034255733E-4 76-77 0.0 0.0 0.0 1.6579570003086102 4.3205431539965027E-4 78-79 0.0 0.0 0.0 1.8921921613002777 4.526283304186812E-4 80-81 0.0 0.0 0.0 2.151856804855467 4.526283304186812E-4 82-83 0.0 0.0 0.0 2.442649933134451 5.143503754757742E-4 84-85 0.0 0.0 0.0 2.7563830881596543 5.76072420532867E-4 86-87 0.0 0.0 0.0 3.1352330007200906 5.76072420532867E-4 88-89 0.0 0.0 0.0 3.584836950930974 5.76072420532867E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4360 0.0 44.283062 1 GTATCAA 7880 0.0 43.51181 1 ATCAACG 9985 0.0 34.18182 3 TCAACGC 10225 0.0 33.287216 4 TATCAAC 10390 0.0 32.84942 2 CAACGCA 10380 0.0 32.834595 5 AACGCAG 10650 0.0 31.979067 6 GTATCGA 145 2.3754183E-8 29.517027 1 CGCAGAG 12240 0.0 27.631456 8 ACGCAGA 12325 0.0 27.517406 7 TGTACGC 175 4.9276423E-9 27.163189 2 GTACATG 7830 0.0 25.75148 1 GTGTACG 280 0.0 25.476004 1 TACATGG 7810 0.0 24.954659 2 GCAGAGT 13460 0.0 24.59761 9 TAGCGCG 100 0.0016467816 23.76828 4 GTATAGG 685 0.0 22.90981 1 ACATGGG 7955 0.0 22.707129 3 AGTACTT 8300 0.0 22.289879 12-13 GAGTACT 7955 0.0 22.211664 12-13 >>END_MODULE