FastQCFastQC Report
Thu 26 May 2016
SRR937161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430250
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90390.3719370435140418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74570.30684085999382776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51400.21150087439563833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52300.046.540171
GTATCAA87050.039.2662851
ATCAACG113300.029.7709773
TCAACGC114250.029.6065924
TATCAAC115300.029.4193822
CAACGCA117000.028.9107115
AACGCAG120800.028.0735686
ACGCAGA137150.024.5541957
CGCAGAG138350.024.2382248
GTACATG78400.022.799821
TACATGG79200.022.4342252
GAGTACT94600.021.615112-13
GCAGAGT153600.021.4916069
ACCGTCG1553.9100003E-521.4507088
ACATGGG80650.020.7938523
GGGTACG1150.003706311820.6695881
AGAGTAC133500.020.52909510-11
AGTACTT98000.020.38051612-13
GTACTTT99500.020.21606614-15
TAAGGTG11900.019.5620235
Produced by FastQC (version 0.11.5)