##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937161_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2430250 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.982070157391213 31.0 31.0 34.0 30.0 34.0 2 32.22836745190824 33.0 31.0 34.0 30.0 34.0 3 32.3060536981792 34.0 31.0 34.0 30.0 34.0 4 35.96955909885814 37.0 35.0 37.0 35.0 37.0 5 35.843140417652506 37.0 35.0 37.0 35.0 37.0 6 35.92050735521037 37.0 35.0 37.0 35.0 37.0 7 35.87407098035182 37.0 35.0 37.0 35.0 37.0 8 35.84588869457875 37.0 35.0 37.0 35.0 37.0 9 37.51701388746014 39.0 37.0 39.0 35.0 39.0 10-11 37.42575002571752 39.0 37.0 39.0 34.5 39.0 12-13 37.341720604876045 39.0 37.0 39.0 34.0 39.0 14-15 38.508707951856806 40.0 38.0 41.0 34.0 41.0 16-17 38.47007262627302 40.0 38.0 41.0 34.0 41.0 18-19 38.45523711552309 40.0 38.0 41.0 34.0 41.0 20-21 38.32893755786442 40.0 38.0 41.0 34.0 41.0 22-23 38.190440901141855 40.0 38.0 41.0 33.5 41.0 24-25 38.04247155642423 40.0 37.0 41.0 33.0 41.0 26-27 37.98394321571855 40.0 37.0 41.0 33.0 41.0 28-29 37.88144902787779 40.0 37.0 41.0 33.0 41.0 30-31 37.9807026026129 40.0 37.0 41.0 33.0 41.0 32-33 37.91882152041971 40.0 37.0 41.0 33.0 41.0 34-35 37.799367554778314 40.0 37.0 41.0 33.0 41.0 36-37 37.62654850324041 40.0 37.0 41.0 33.0 41.0 38-39 37.48068820080239 40.0 37.0 41.0 32.5 41.0 40-41 37.310009875527214 40.0 36.0 41.0 32.0 41.0 42-43 37.1010264376093 39.0 36.0 41.0 31.5 41.0 44-45 36.82235860508178 39.0 36.0 41.0 31.0 41.0 46-47 36.500297088776875 39.0 36.0 40.5 30.0 41.0 48-49 36.210639234646635 39.0 35.0 40.0 30.0 41.0 50-51 35.98445303981072 39.0 35.0 40.0 29.0 41.0 52-53 35.734474436786336 39.0 35.0 40.0 28.5 41.0 54-55 35.482049994856496 38.0 35.0 40.0 28.0 41.0 56-57 35.203687892192164 38.0 34.0 40.0 27.5 41.0 58-59 34.936462503857626 38.0 34.0 40.0 27.0 41.0 60-61 34.581365908857116 37.5 34.0 40.0 26.0 41.0 62-63 34.174717004423414 37.0 33.5 40.0 26.0 41.0 64-65 33.792503446147514 36.5 33.0 39.0 25.5 40.5 66-67 33.34133278469294 36.0 32.5 39.0 24.5 40.0 68-69 32.90617549634811 35.5 32.0 38.5 23.0 40.0 70-71 32.491992593354595 35.0 31.5 38.0 22.5 40.0 72-73 32.02231848575249 35.0 31.0 37.0 21.5 39.5 74-75 31.552907108322188 34.5 31.0 37.0 20.0 39.0 76-77 30.298232074889413 33.5 29.5 35.5 19.5 38.0 78-79 30.96149367349038 34.0 30.5 36.0 20.0 38.0 80-81 30.8300545211398 34.0 31.0 35.5 19.5 37.0 82-83 30.482090114185784 34.0 30.0 35.0 18.0 37.0 84-85 30.091877378870485 34.0 30.0 35.0 16.5 36.5 86-87 29.67979261392861 34.0 29.5 35.0 10.0 36.0 88-89 29.234892500771526 34.0 29.0 35.0 7.0 35.5 90-91 28.72106038473408 33.5 29.0 35.0 2.0 35.0 92-93 28.27391461783767 33.0 28.5 35.0 2.0 35.0 94-95 27.767058738812878 33.0 27.0 35.0 2.0 35.0 96-97 27.172067071288964 33.0 26.5 34.0 2.0 35.0 98-99 26.518298323217778 32.5 25.0 34.0 2.0 35.0 100-101 24.464826663923464 30.5 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06379582693078234 1101 2 0.07549931705617752 1101 3 0.03985038041400202 1101 4 -0.02764199895526076 1101 5 -0.07012510752208811 1101 6 -0.06153424071029434 1101 7 -0.04374365028787963 1101 8 -0.07692802912044527 1101 9 0.007229965952674888 1101 10-11 -0.07817505685826376 1101 12-13 -0.07385937692230016 1101 14-15 -0.08760780267800783 1101 16-17 -0.10357399590292005 1101 18-19 -0.11742615838055315 1101 20-21 -0.10900962974314865 1101 22-23 -0.17156951000720255 1101 24-25 -0.045485707663679875 1101 26-27 -0.07943693303306532 1101 28-29 -0.049177347994024956 1101 30-31 -0.0074934039966905175 1101 32-33 -0.09053462220454378 1101 34-35 -0.10306902480949987 1101 36-37 0.011489452555345281 1101 38-39 -0.10651297184471531 1101 40-41 -0.1724143085700902 1101 42-43 0.06964164466766931 1101 44-45 -0.03406188118923126 1101 46-47 -0.06976409835670694 1101 48-49 0.09905039929498827 1101 50-51 -0.08857777359578733 1101 52-53 0.0015425325799753864 1101 54-55 -0.00922974288275924 1101 56-57 -0.011874674616365155 1101 58-59 -0.13014334100462577 1101 60-61 -0.06331158351478194 1101 62-63 -0.1764525831003283 1101 64-65 -0.06265250997158489 1101 66-67 -0.07240752200090839 1101 68-69 -0.0942690033759419 1101 70-71 -0.2786372831137456 1101 72-73 -0.26001571381207 1101 74-75 -0.2762837282451933 1101 76-77 -0.15979796794472279 1101 78-79 -0.10059722130322513 1101 80-81 -0.14774323944977397 1101 82-83 -0.12004378668409643 1101 84-85 -0.1118959141230178 1101 86-87 -0.1369550014809171 1101 88-89 -0.0456103386047495 1101 90-91 -0.20880742246857764 1101 92-93 -0.3967686746332646 1101 94-95 -0.4735837865831023 1101 96-97 -0.4862695279296716 1101 98-99 -0.19420073980807473 1101 100-101 0.10113666959404455 1102 1 0.07300733341933707 1102 2 0.07209634355363193 1102 3 0.07948033230281482 1102 4 0.07623112526086828 1102 5 -0.059281672197897706 1102 6 0.045151687598206536 1102 7 -0.022438185896703544 1102 8 -0.043128067672341786 1102 9 0.01700678696639102 1102 10-11 -0.017806162085271637 1102 12-13 0.027010195369832957 1102 14-15 -0.03507847838398703 1102 16-17 -0.03661913773848369 1102 18-19 -0.03114743176605117 1102 20-21 -0.06933613710251763 1102 22-23 -0.08572055832902947 1102 24-25 -0.06600998659485668 1102 26-27 -0.14236033778753665 1102 28-29 -0.06713844455578766 1102 30-31 -0.0797138321372799 1102 32-33 -0.11587963125422363 1102 34-35 -0.14525169855855324 1102 36-37 -0.1309807153242062 1102 38-39 -0.17326005032285963 1102 40-41 -0.08140850468621608 1102 42-43 -0.12855415889791288 1102 44-45 -0.10307151415589999 1102 46-47 0.02583297498951964 1102 48-49 0.043550871537796354 1102 50-51 -0.019282398857434657 1102 52-53 -0.01188254746526951 1102 54-55 0.06366516458375315 1102 56-57 -0.023378150187589597 1102 58-59 -0.06234189684640512 1102 60-61 -0.07071668523921204 1102 62-63 -0.0774420757423897 1102 64-65 -0.11676548382818197 1102 66-67 -0.04628311561244658 1102 68-69 0.014861867785995742 1102 70-71 -0.08163956547168283 1102 72-73 -0.0749938933687666 1102 74-75 -0.1943268459275309 1102 76-77 -0.08758192070112969 1102 78-79 -0.06766011775205172 1102 80-81 0.018439874534735168 1102 82-83 0.09459803832964653 1102 84-85 0.06261573208933058 1102 86-87 0.07063192535023788 1102 88-89 0.009713506242118086 1102 90-91 0.06737845058697545 1102 92-93 0.19085874241489975 1102 94-95 0.1239057895642226 1102 96-97 0.09574488718839191 1102 98-99 0.08653478418833416 1102 100-101 0.2909969672590087 1103 1 0.04926307197283819 1103 2 0.061042923414646566 1103 3 0.07613555368557456 1103 4 0.05509874241106871 1103 5 -0.07642500410001674 1103 6 -0.10518528990422737 1103 7 -0.020655436780231184 1103 8 -0.10725561212994705 1103 9 -0.008339033182721778 1103 10-11 -0.05375153201286764 1103 12-13 0.02544530402639822 1103 14-15 -0.08689455455264294 1103 16-17 0.06695586717807345 1103 18-19 0.02209643559577046 1103 20-21 0.10745754738308477 1103 22-23 0.01443011768739666 1103 24-25 0.03505106840376726 1103 26-27 -0.02458791026437268 1103 28-29 -0.1683304288564358 1103 30-31 -0.11756085180091702 1103 32-33 -0.1757295705339672 1103 34-35 -0.1179953748476592 1103 36-37 -0.1821869507070133 1103 38-39 -0.1377063056777459 1103 40-41 -0.06129073449451283 1103 42-43 -0.09555031606924302 1103 44-45 -0.20574767689350892 1103 46-47 -0.1807422516304129 1103 48-49 -0.03732721691584828 1103 50-51 -0.12140130716819897 1103 52-53 -0.08131138716604625 1103 54-55 -0.11203283660695007 1103 56-57 -0.20509576913091365 1103 58-59 -0.22127951645689592 1103 60-61 -0.28224075329132603 1103 62-63 -0.24587672024384233 1103 64-65 -0.32645873275027526 1103 66-67 -0.20627308843120318 1103 68-69 -0.05888437459222473 1103 70-71 -0.10133655535873487 1103 72-73 -0.1785118850134353 1103 74-75 -0.1498316315992163 1103 76-77 -0.08055770673975715 1103 78-79 -0.1828104713640144 1103 80-81 -0.16148488941020034 1103 82-83 -0.18935439355245975 1103 84-85 -0.14305550997810812 1103 86-87 -0.10031054293719066 1103 88-89 -0.15235228626874786 1103 90-91 -0.05870570708374245 1103 92-93 -0.054773258423622906 1103 94-95 0.03228376242626041 1103 96-97 0.010815457992752187 1103 98-99 0.23825631222987553 1103 100-101 0.6649200983438313 1104 1 -0.0200227369602608 1104 2 0.06942824243585477 1104 3 0.029965773120039785 1104 4 0.03869362661806974 1104 5 -0.029090867051586144 1104 6 0.024300063084155 1104 7 -0.07886458383894279 1104 8 -0.02485167605910732 1104 9 0.00464658762030723 1104 10-11 -0.10274751181044195 1104 12-13 0.04544201034915574 1104 14-15 0.020068111019263313 1104 16-17 -2.6651261762111744E-4 1104 18-19 -0.07804170180625647 1104 20-21 -0.23790150411017663 1104 22-23 -0.15864231736145484 1104 24-25 -0.13763912292778713 1104 26-27 -0.14307342974434079 1104 28-29 -0.057511657210049805 1104 30-31 -0.17061436357129622 1104 32-33 -0.0933668506218126 1104 34-35 -0.21260751904420516 1104 36-37 -0.2624240105053346 1104 38-39 -0.29458291444112916 1104 40-41 -0.1241872705475302 1104 42-43 -0.25457802869355106 1104 44-45 -0.29602213674037614 1104 46-47 -0.340684854513988 1104 48-49 -0.417282623924514 1104 50-51 -0.5321584715037133 1104 52-53 -0.3903071663059947 1104 54-55 -0.3906833093002504 1104 56-57 -0.2773676254904345 1104 58-59 -0.2889807177452255 1104 60-61 -0.2451887372336543 1104 62-63 -0.3245601297095675 1104 64-65 -0.43656168999157785 1104 66-67 -0.20021806413627274 1104 68-69 -0.21573685021006384 1104 70-71 -0.43364404923370614 1104 72-73 -0.3032982795594563 1104 74-75 -0.5114142579760035 1104 76-77 -0.353977478311851 1104 78-79 -0.3217056722304221 1104 80-81 -0.3250982612336415 1104 82-83 -0.25994172019829165 1104 84-85 -0.25449487404893745 1104 86-87 -0.20412066949429786 1104 88-89 -0.23267295340650307 1104 90-91 -0.3220434277687012 1104 92-93 -0.41353000192045997 1104 94-95 -0.1929147756293581 1104 96-97 -0.22023848618574604 1104 98-99 -0.22691097204646837 1104 100-101 0.020775441196288824 1105 1 -0.03559077532082355 1105 2 0.044256864461296175 1105 3 0.04842265149959957 1105 4 0.012665739509138518 1105 5 -0.06676254707861773 1105 6 -0.00981541863319535 1105 7 -0.006733843750481583 1105 8 0.0020727231001416158 1105 9 0.058470003377919966 1105 10-11 0.010482569326434543 1105 12-13 0.0011174595849468005 1105 14-15 0.042854492808309885 1105 16-17 0.030927334812069773 1105 18-19 0.07556832783855327 1105 20-21 0.07000632104905691 1105 22-23 0.04977683441022407 1105 24-25 -0.010299383848334287 1105 26-27 0.06616009611063589 1105 28-29 0.044382064586123704 1105 30-31 0.020209698527857256 1105 32-33 0.006168019026553395 1105 34-35 -0.07641302283625606 1105 36-37 -0.11301926201706891 1105 38-39 -0.2353574297814376 1105 40-41 -0.06388455000404036 1105 42-43 -0.28451995745546554 1105 44-45 -0.06381703793216076 1105 46-47 -0.16417549068422232 1105 48-49 -0.1256874599318465 1105 50-51 -0.16920318197093565 1105 52-53 -0.05294232082792405 1105 54-55 -0.3190477829745717 1105 56-57 -0.048603386635257095 1105 58-59 -0.24165203937049284 1105 60-61 -0.24901495522599504 1105 62-63 -0.2143307241549337 1105 64-65 -0.2828718575753655 1105 66-67 -0.16813090285892685 1105 68-69 -0.03013453930049792 1105 70-71 -0.09001367089749834 1105 72-73 -0.06257154435175494 1105 74-75 -0.03413890937698838 1105 76-77 -0.08178413467522816 1105 78-79 -0.05736538833967586 1105 80-81 -0.09105241911032635 1105 82-83 -0.09228193545459007 1105 84-85 -0.021415447548609023 1105 86-87 0.19178950954210094 1105 88-89 0.27366451362916067 1105 90-91 0.11953627316787063 1105 92-93 0.0582427628353841 1105 94-95 0.11145365447549338 1105 96-97 0.09699067833609476 1105 98-99 0.2606543144383764 1105 100-101 0.6843326426247813 1106 1 -0.1558242791082236 1106 2 -0.09627895646763562 1106 3 -0.11422472222070468 1106 4 0.011898736794066167 1106 5 -0.17462866851185765 1106 6 -0.17629084688410757 1106 7 -0.18563244269759593 1106 8 -0.07132652553179497 1106 9 -0.25767255636518627 1106 10-11 -0.29675513876784265 1106 12-13 -0.362657267785778 1106 14-15 -0.3421238639826072 1106 16-17 -0.17397843218310527 1106 18-19 -0.2686612844510279 1106 20-21 -0.25912581764053044 1106 22-23 -0.20346378975578006 1106 24-25 -0.1639171070507075 1106 26-27 -0.18746623311039912 1106 28-29 -0.2033203198029767 1106 30-31 -0.21673319079561537 1106 32-33 -0.23720821720424823 1106 34-35 -0.44889090554224964 1106 36-37 -0.2800853971828232 1106 38-39 -0.28392732868855575 1106 40-41 -0.36552724411470905 1106 42-43 -0.5787007578453611 1106 44-45 -0.4815578234900073 1106 46-47 -0.3043864402076011 1106 48-49 -0.26424343263058603 1106 50-51 -0.38110046925410046 1106 52-53 -0.23252679162678902 1106 54-55 -0.28527055150758684 1106 56-57 -0.16861205528532963 1106 58-59 -0.2846172976154264 1106 60-61 -0.2634875066078095 1106 62-63 -0.061365353405442136 1106 64-65 -0.1758086939217165 1106 66-67 -0.3662297962200327 1106 68-69 -0.4402452542228801 1106 70-71 -0.340351213563693 1106 72-73 -0.2870070005455503 1106 74-75 -0.2734160362843312 1106 76-77 -0.22354967680279003 1106 78-79 -0.27020811984360904 1106 80-81 -0.22718212085842993 1106 82-83 -0.2743709084654462 1106 84-85 -0.3680601383251947 1106 86-87 -0.2964724377169219 1106 88-89 -0.2926432944306434 1106 90-91 -0.27391414068071995 1106 92-93 -0.4838179355766634 1106 94-95 -1.0416400526575167 1106 96-97 -0.49233625793401714 1106 98-99 -0.27824233829589673 1106 100-101 0.03846880306231171 1107 1 -0.046352398890888935 1107 2 -0.05673044224698742 1107 3 -0.03561734907387404 1107 4 -0.06956250079493742 1107 5 -0.19823241348994713 1107 6 -0.12277830757894748 1107 7 -0.15344645627816078 1107 8 -0.11415809000995836 1107 9 -0.18796599557418858 1107 10-11 -0.14048356886217306 1107 12-13 -0.2212361170368382 1107 14-15 -0.2382188630091946 1107 16-17 -0.19246734414544164 1107 18-19 -0.14105003535807015 1107 20-21 -0.08518314860621246 1107 22-23 -0.12416322989689377 1107 24-25 -0.1640321465475978 1107 26-27 -0.23722671497840508 1107 28-29 -0.2534377190844239 1107 30-31 -0.27521160171669834 1107 32-33 -0.25478492495083316 1107 34-35 -0.14830523651382777 1107 36-37 -0.38143076935033804 1107 38-39 -0.2314899662197476 1107 40-41 -0.16225761513183556 1107 42-43 -0.22080108408557209 1107 44-45 -0.15265916443757988 1107 46-47 -0.14282010371412213 1107 48-49 -0.028589447839401316 1107 50-51 -0.004983258755423492 1107 52-53 -0.08511695014531284 1107 54-55 -0.09151970346001548 1107 56-57 -0.040683710143234464 1107 58-59 -0.03444400093741251 1107 60-61 0.007545548074297415 1107 62-63 -0.1366041341047648 1107 64-65 0.017237905923714436 1107 66-67 0.0260961844179306 1107 68-69 -0.07549409350385616 1107 70-71 -0.12236316587244289 1107 72-73 -0.19847083389756648 1107 74-75 -0.07661442927105 1107 76-77 -0.09081105833865522 1107 78-79 -0.045291892195194805 1107 80-81 0.015645608969840907 1107 82-83 0.04459084532643587 1107 84-85 0.008588023697612357 1107 86-87 -0.13511184493904338 1107 88-89 -0.16293109965402408 1107 90-91 0.0838380465445887 1107 92-93 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64-65 -0.26422896397179585 2316 66-67 -0.18796371418180513 2316 68-69 -0.17109477430570763 2316 70-71 -0.17689266931688508 2316 72-73 0.017765171503654642 2316 74-75 -0.030158786070597188 2316 76-77 0.0993613741710142 2316 78-79 -0.09949337343215703 2316 80-81 -0.01240672836492962 2316 82-83 -0.19998633418659395 2316 84-85 -0.39915147266852813 2316 86-87 -0.42370852467305653 2316 88-89 -0.3238573546124357 2316 90-91 -0.2743322957062233 2316 92-93 -0.2730873205664359 2316 94-95 -0.1753514801482794 2316 96-97 0.0938538026925464 2316 98-99 0.04617794476607173 2316 100-101 -0.26539297984544064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 25.0 5 46.0 6 108.0 7 258.0 8 680.0 9 1224.0 10 2162.0 11 3490.0 12 5029.0 13 7012.0 14 9311.0 15 12567.0 16 16432.0 17 21319.0 18 19181.0 19 13502.0 20 12008.0 21 12534.0 22 13268.0 23 14892.0 24 17140.0 25 21086.0 26 25800.0 27 32085.0 28 40034.0 29 50202.0 30 62637.0 31 79486.0 32 100847.0 33 132687.0 34 179792.0 35 254593.0 36 379072.0 37 522115.0 38 343388.0 39 24226.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80507222421433 20.248798595984493 15.537746914101207 22.408382265699974 2 16.047279086513733 21.00471144943936 39.049315914000616 23.89869355004629 3 19.5239995885197 24.422219936220554 30.165044748482668 25.888735726777078 4 13.566875835819362 17.37496142372184 36.81674724822549 32.24141549223331 5 13.632427734103313 37.66386840976753 34.45201876991381 14.25168508621534 6 32.18187429276823 37.02989404382265 16.77498199773686 14.013249665672255 7 28.513280526694786 32.84188869457875 20.525789527826355 18.119041250900114 8 26.46567225594075 35.63925522065631 19.291883551074992 18.60318897232795 9 26.076370743750644 16.672687995062237 20.209854953194114 37.041086307993005 10-11 24.851270445427424 27.1554572574838 27.143442032712684 20.84983026437609 12-13 25.69085644966694 24.139525926559934 27.921727350985858 22.247890272787277 14-15 22.56430408394198 25.695216541508074 26.070630593560335 25.66984878098961 16-17 21.96006583684806 28.96311079107088 26.859335459314888 22.217487912766178 18-19 21.96154716592943 27.398251208723384 29.39246991050303 21.24773171484415 20-21 22.494331858862257 26.49568974385351 29.74586976648493 21.2641086307993 22-23 22.30082494453186 26.2878236738671 29.61175595336068 21.79959542824036 24-25 22.245829795454906 26.491078168408023 29.659007841385638 21.604084194751437 26-27 21.87405744091376 26.91477599343927 29.506517725357263 21.704648840289703 28-29 21.930767209695972 27.070483117699695 29.485690983343304 21.51305868926103 30-31 22.066762678736758 26.71122312519288 29.45266947844872 21.769344717621646 32-33 22.02448307787265 26.700894969653326 29.299249048451802 21.97537290402222 34-35 22.307972430819873 26.836230840448515 29.240201625347183 21.615595103384425 36-37 22.4445427425162 26.62880362102664 29.18635942804238 21.740294208414774 38-39 22.1625964406954 26.901635634194015 29.23104618866372 21.704721736446867 40-41 22.389918732640673 26.789301512190107 29.113321674724823 21.7074580804444 42-43 22.1621277927568 26.776060294347932 29.003125463628958 22.05868644926631 44-45 22.31422693138566 26.950396049789116 28.971566711243703 21.763810307581526 46-47 22.64304083941981 27.20106984878099 28.33994445015945 21.81594486163975 48-49 22.505174364777286 27.285402736344 28.212426705071497 21.996996193807224 50-51 22.579590577101122 27.490052463738294 28.12797037341837 21.80238658574221 52-53 22.71821829029935 27.43863800020574 27.94241333196173 21.900730377533176 54-55 22.53179714021191 27.555086925213455 27.94947021911326 21.96364571546137 56-57 22.50511264273223 27.707602098549533 27.892891677810926 21.894393580907316 58-59 22.52397901450468 27.374323629256246 27.96156773994445 22.140129616294622 60-61 22.38642115008744 27.548235778212117 28.082110893940953 21.98323217775949 62-63 22.35395535438741 27.493858656516817 28.317539347803724 21.83464664129205 64-65 22.26581627404588 27.509968110276724 28.143935809073138 22.08027980660426 66-67 22.27495113671433 27.561938072214794 27.893364880156362 22.269745910914516 68-69 22.35130130644995 27.44337002366012 28.095257689538112 22.110070980351814 70-71 22.343380310667627 27.443390597675137 27.902540890854848 22.310688200802385 72-73 22.38432720591034 27.447284733522213 27.825023726987702 22.34336433357974 74-75 22.30854850324041 27.699413640571958 27.627095977780066 22.36494187840757 76-77 22.46201008126736 27.47052772348524 27.81824915132188 22.249213043925522 78-79 22.43036724616809 27.537866474642524 27.73969756197922 22.292068717210164 80-81 22.434646641292048 27.593724925419195 27.644522168501183 22.327106264787574 82-83 22.3737868242421 27.61972205665811 27.666610362294975 22.339880756804824 84-85 22.36804557276316 27.619373279865002 27.62371444437106 22.388866703000772 86-87 22.384302026540478 27.570682028597883 27.566546651579056 22.478469293282586 88-89 22.362987346980763 27.436354284538627 27.580619277852076 22.620039090628534 90-91 22.324440418817055 27.482266366034057 27.531916837415178 22.661376377733706 92-93 22.236765926419096 27.36975166476492 27.698980710823655 22.694501697992326 94-95 22.177800637794466 27.400781812570724 27.617631930871312 22.803785618763502 96-97 22.22250797243082 27.37389157494085 27.46663923464664 22.93696121798169 98-99 21.9676526573853 27.476415967766226 27.45026635163683 23.105665023211643 100-101 22.085210282816128 27.371890738699822 27.52420040511655 23.018698573367498 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 350.0 1 261.5 2 259.0 3 374.0 4 320.5 5 271.0 6 528.0 7 1226.0 8 2024.5 9 2448.5 10 2357.5 11 2175.5 12 2268.5 13 2476.0 14 2727.5 15 2953.0 16 3148.0 17 3287.0 18 3520.5 19 3941.0 20 4379.0 21 5039.0 22 5997.5 23 7384.0 24 9239.5 25 11713.0 26 15356.0 27 19741.5 28 23567.0 29 27358.5 30 31344.0 31 36008.5 32 42157.5 33 46146.0 34 49278.0 35 53571.0 36 59205.5 37 64316.5 38 67511.0 39 70025.0 40 72613.5 41 76315.0 42 78050.0 43 80269.0 44 84599.0 45 87521.5 46 88213.5 47 89694.0 48 91495.0 49 90351.5 50 87595.0 51 84511.5 52 82982.0 53 82812.5 54 81338.0 55 75063.5 56 67546.5 57 61639.0 58 56674.5 59 50217.0 60 40515.0 61 33009.5 62 26963.5 63 19831.0 64 13935.5 65 10285.5 66 7550.5 67 5791.5 68 4598.0 69 3576.0 70 2653.5 71 1750.0 72 1193.5 73 961.0 74 691.5 75 455.0 76 323.0 77 201.5 78 129.0 79 83.0 80 56.0 81 44.5 82 27.5 83 12.5 84 10.0 85 9.0 86 5.5 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050077152556321364 2 0.0 3 0.0 4 0.0 5 0.016623804135377018 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0032095463429688306 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010698487809896102 24-25 1.4401810513321676E-4 26-27 0.0012755889311799199 28-29 0.0035593045982923567 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015245345129101943 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.0574015019030964E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.6746219524740255E-4 84-85 0.0010904227960086411 86-87 0.0 88-89 0.0 90-91 0.010821931900010287 92-93 0.001110996811027672 94-95 0.0 96-97 0.0 98-99 1.6459212015224772E-4 100-101 0.01366114597263656 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2430250.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.466118875386904 #Duplication Level Percentage of deduplicated Percentage of total 1 73.15192857803797 38.379977807391526 2 14.514416902797722 15.230302452582206 3 5.243312571411347 8.252887820174351 4 2.3803035637344814 4.995411589376015 5 1.2453598543338653 3.266959908005755 6 0.8030652174950652 2.528022909947072 7 0.518166340522606 1.9030323773358282 8 0.3418732999100704 1.4349412154722045 9 0.2651779817511255 1.2521573562320758 >10 1.3973526588988416 13.260218966080215 >50 0.09363625088779312 3.3403135598342875 >100 0.0418768133654978 3.8897043595772596 >500 0.00274552977506003 0.9502594041984053 >1k 5.491059550113556E-4 0.42535107377458187 >5k 2.3533112357629527E-4 0.8904592000180795 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9039 0.3719370435140418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7457 0.30684085999382776 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5140 0.21150087439563833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.0574015019030964E-5 2.0574015019030964E-5 18-19 0.0 0.0 0.0 4.114803003806193E-5 4.114803003806193E-5 20-21 0.0 0.0 0.0 4.114803003806193E-5 4.114803003806193E-5 22-23 0.0 0.0 0.0 1.2344409011418579E-4 6.172204505709289E-5 24-25 0.0 0.0 0.0 1.851661351712787E-4 8.229606007612386E-5 26-27 0.0 0.0 0.0 2.6746219524740255E-4 8.229606007612386E-5 28-29 0.0 0.0 0.0 6.995165106470528E-4 8.229606007612386E-5 30-31 0.0 0.0 0.0 0.0018105133216747248 8.229606007612386E-5 32-33 0.0 0.0 0.0 0.003538730583273326 1.2344409011418579E-4 34-35 0.0 0.0 0.0 0.0063779446558995985 1.4401810513321674E-4 36-37 0.0 0.0 0.0 0.009896101224153894 1.6459212015224772E-4 38-39 0.0 0.0 0.0 0.014175496348112333 1.6459212015224772E-4 40-41 0.0 0.0 0.0 0.02154099372492542 1.6459212015224772E-4 42-43 0.0 0.0 0.0 0.033185886225696945 1.6459212015224772E-4 44-45 0.0 0.0 0.0 0.04841065733977986 1.6459212015224772E-4 46-47 0.0 0.0 0.0 0.07713198230634707 1.6459212015224772E-4 48-49 0.0 0.0 0.0 0.11593457463223948 1.6459212015224772E-4 50-51 4.114803003806193E-5 0.0 0.0 0.1567534204299969 2.0574015019030964E-4 52-53 4.114803003806193E-5 0.0 0.0 0.19870383705380104 2.4688818022837157E-4 54-55 4.114803003806193E-5 0.0 0.0 0.2472379384836951 2.4688818022837157E-4 56-57 4.114803003806193E-5 0.0 0.0 0.3035078695607448 2.4688818022837157E-4 58-59 4.114803003806193E-5 0.0 0.0 0.37395329698590685 2.4688818022837157E-4 60-61 4.114803003806193E-5 0.0 0.0 0.46069334430614134 2.4688818022837157E-4 62-63 4.114803003806193E-5 0.0 0.0 0.5487089805575558 2.880362102664335E-4 64-65 4.114803003806193E-5 0.0 0.0 0.6457566094023248 3.0861022528546445E-4 66-67 4.114803003806193E-5 0.0 0.0 0.7783767102149984 3.2918424030449543E-4 68-69 4.114803003806193E-5 0.0 0.0 0.9343071700442341 3.2918424030449543E-4 70-71 4.114803003806193E-5 0.0 0.0 1.1062030655282378 3.2918424030449543E-4 72-73 4.114803003806193E-5 0.0 0.0 1.2765353358707952 3.703322703425574E-4 74-75 4.114803003806193E-5 0.0 0.0 1.460425882110894 4.114803003806193E-4 76-77 4.114803003806193E-5 0.0 0.0 1.656537393272297 4.114803003806193E-4 78-79 4.114803003806193E-5 0.0 0.0 1.8929328258409628 4.114803003806193E-4 80-81 4.114803003806193E-5 0.0 0.0 2.1527826355313238 4.114803003806193E-4 82-83 4.114803003806193E-5 0.0 0.0 2.44458389054624 4.526283304186812E-4 84-85 4.114803003806193E-5 0.0 0.0 2.7591400061722045 4.526283304186812E-4 86-87 4.114803003806193E-5 0.0 0.0 3.1388540273634398 4.937763604567431E-4 88-89 4.114803003806193E-5 0.0 0.0 3.5888694578747042 5.349243904948051E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5230 0.0 46.54017 1 GTATCAA 8705 0.0 39.266285 1 ATCAACG 11330 0.0 29.770977 3 TCAACGC 11425 0.0 29.606592 4 TATCAAC 11530 0.0 29.419382 2 CAACGCA 11700 0.0 28.910711 5 AACGCAG 12080 0.0 28.073568 6 ACGCAGA 13715 0.0 24.554195 7 CGCAGAG 13835 0.0 24.238224 8 GTACATG 7840 0.0 22.79982 1 TACATGG 7920 0.0 22.434225 2 GAGTACT 9460 0.0 21.6151 12-13 GCAGAGT 15360 0.0 21.491606 9 ACCGTCG 155 3.9100003E-5 21.450708 8 ACATGGG 8065 0.0 20.793852 3 GGGTACG 115 0.0037063118 20.669588 1 AGAGTAC 13350 0.0 20.529095 10-11 AGTACTT 9800 0.0 20.380516 12-13 GTACTTT 9950 0.0 20.216066 14-15 TAAGGTG 1190 0.0 19.562023 5 >>END_MODULE