FastQCFastQC Report
Thu 26 May 2016
SRR937160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799856
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96870.3459820790783526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70950.25340588944574294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.18122360578544042No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40980.146364670183038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.044.66331
GTATCAA91750.041.17031
ATCAACG115600.032.217783
TCAACGC117850.031.568024
TATCAAC118600.031.4434452
CAACGCA120400.030.8583075
AACGCAG125000.029.7216596
ACGCAGA143550.025.6492567
CGCAGAG143950.025.3799618
GTACATG90300.023.0993481
GCAGAGT157100.022.4692679
TGGTATC28150.022.4449372
TACATGG91500.022.16932
GAGTACT93600.021.69767812-13
GGACCGA7400.021.1864646
ACATGGG91450.020.6745833
AGGACCG8100.020.5293335
GTGGTAT32700.020.4877511
AGAGTAC139200.020.47675310-11
TAAGGTG12750.020.1221985
Produced by FastQC (version 0.11.5)