##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937160_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2799856 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.875736109285622 33.0 31.0 34.0 30.0 34.0 2 32.000215725380166 33.0 31.0 34.0 30.0 34.0 3 32.05228054585665 34.0 31.0 34.0 30.0 34.0 4 35.65190424078953 37.0 35.0 37.0 35.0 37.0 5 35.6648720505626 37.0 35.0 37.0 35.0 37.0 6 35.681927570560774 37.0 35.0 37.0 35.0 37.0 7 35.5805670005886 37.0 35.0 37.0 33.0 37.0 8 35.582530315844814 37.0 35.0 37.0 33.0 37.0 9 37.2358607014075 39.0 37.0 39.0 34.0 39.0 10-11 37.13882713968147 39.0 37.0 39.0 34.0 39.0 12-13 36.993166077112534 39.0 37.0 39.0 33.0 39.0 14-15 38.21348580069832 40.0 38.0 41.0 33.0 41.0 16-17 38.13767922350292 40.0 38.0 41.0 33.0 41.0 18-19 38.09257583247138 40.0 38.0 41.0 33.5 41.0 20-21 37.9681447903035 40.0 38.0 41.0 33.0 41.0 22-23 37.82582282803116 40.0 38.0 41.0 33.0 41.0 24-25 37.765289357738396 40.0 37.5 41.0 33.0 41.0 26-27 37.780182445097175 40.0 38.0 41.0 33.0 41.0 28-29 37.700693535667554 40.0 37.5 41.0 33.0 41.0 30-31 37.49579031921642 40.0 37.0 41.0 32.5 41.0 32-33 37.27071945842929 40.0 37.0 41.0 32.0 41.0 34-35 37.323340736095 40.0 37.0 41.0 32.0 41.0 36-37 37.20642865204496 40.0 37.0 41.0 31.5 41.0 38-39 37.089679612094336 40.0 37.0 41.0 31.0 41.0 40-41 36.88694954311936 39.5 36.5 41.0 31.0 41.0 42-43 36.63568983547725 39.0 36.0 41.0 30.0 41.0 44-45 36.4306782205942 39.0 36.0 41.0 30.0 41.0 46-47 36.13876052911293 39.0 35.5 41.0 29.5 41.0 48-49 35.83543439376882 39.0 35.0 40.5 28.5 41.0 50-51 34.87052280545856 38.0 34.0 39.5 27.0 40.5 52-53 34.99142348749364 38.0 34.5 39.5 27.0 40.5 54-55 35.36436373870656 39.0 35.0 40.0 27.5 41.0 56-57 35.14676165488511 38.5 35.0 40.0 26.5 41.0 58-59 34.87645596773548 38.0 34.0 40.0 26.0 41.0 60-61 34.56363023669789 38.0 34.0 40.0 26.0 41.0 62-63 34.24424309678784 37.5 34.0 40.0 25.0 41.0 64-65 33.81973215765382 37.0 33.5 40.0 24.0 41.0 66-67 33.471585324388116 36.5 33.0 39.0 23.0 41.0 68-69 32.984599386539884 36.0 32.5 39.0 22.0 40.5 70-71 32.51907133795453 35.0 32.0 38.5 20.5 40.0 72-73 32.05961235149236 35.0 31.5 38.0 19.5 40.0 74-75 31.57558388717134 35.0 31.0 37.0 18.0 39.0 76-77 31.100719465572517 35.0 31.0 36.5 15.0 39.0 78-79 30.60178577041105 34.0 30.0 36.0 9.5 38.5 80-81 30.121841087541647 34.0 30.0 35.5 7.0 37.5 82-83 29.64958537153339 34.0 29.5 35.0 3.5 37.0 84-85 29.133332392808775 34.0 29.0 35.0 2.0 36.5 86-87 28.675324552405552 34.0 29.0 35.0 2.0 36.0 88-89 28.212278595756352 34.0 27.5 35.0 2.0 36.0 90-91 27.707760327673995 33.0 26.5 35.0 2.0 35.0 92-93 27.200527991439557 33.0 26.0 35.0 2.0 35.0 94-95 26.648563354686814 33.0 25.0 35.0 2.0 35.0 96-97 25.909750894331708 32.0 23.5 34.5 2.0 35.0 98-99 24.982139081438476 32.0 19.0 34.0 2.0 35.0 100-101 22.427923078901202 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.007155631945558127 1101 2 -0.020838086373476727 1101 3 -0.01883559462662987 1101 4 -0.09553628076849918 1101 5 -0.07823700063406136 1101 6 -0.15660426732690524 1101 7 -0.036358474652374184 1101 8 -0.20465763777451684 1101 9 -0.0903618975253977 1101 10-11 -0.2111993764200406 1101 12-13 -0.1174570866283915 1101 14-15 -0.2237331257794395 1101 16-17 -0.2433911687155046 1101 18-19 -0.07787141287575139 1101 20-21 -0.02028346141644022 1101 22-23 -0.17000929232715833 1101 24-25 -0.21364653324756233 1101 26-27 -0.23612956645498429 1101 28-29 -0.2115342293516278 1101 30-31 -0.022123980862851056 1101 32-33 -0.1984617736489227 1101 34-35 -0.2558808273650115 1101 36-37 -0.2158972898943503 1101 38-39 -0.24014398918399138 1101 40-41 -0.23063499097865758 1101 42-43 -0.2879513176463675 1101 44-45 -0.17036118556853097 1101 46-47 -0.054713388062616275 1101 48-49 -0.23895748767320413 1101 50-51 -0.1546809878086819 1101 52-53 -0.01805257624901202 1101 54-55 -0.08946349329702485 1101 56-57 -0.13247809475535632 1101 58-59 -0.15181102041169225 1101 60-61 -0.01473454928959228 1101 62-63 -0.07747932846763206 1101 64-65 -0.22121435840403336 1101 66-67 -0.20472923499976758 1101 68-69 -0.011787454664144548 1101 70-71 -0.09431816806917936 1101 72-73 0.00601781786254918 1101 74-75 -0.1147953313424992 1101 76-77 -0.012482303421311514 1101 78-79 -0.08630884670770556 1101 80-81 0.18416216683288766 1101 82-83 0.18373911464365023 1101 84-85 0.16022746047473646 1101 86-87 0.09702479859583946 1101 88-89 -0.03637994046449222 1101 90-91 -0.10512436947132997 1101 92-93 -0.1606040153500672 1101 94-95 -0.24905067111254198 1101 96-97 0.04760988951816714 1101 98-99 0.1706757376154897 1101 100-101 0.3784571963955621 1102 1 -0.10818345796881701 1102 2 -0.07887484806083478 1102 3 -0.04605759081277938 1102 4 -0.07251299113556797 1102 5 -0.020546144730268168 1102 6 -0.03657579093432872 1102 7 -0.03028988365328189 1102 8 -0.06878036464822657 1102 9 -0.15199674376675176 1102 10-11 -0.13212373633447072 1102 12-13 -0.09015565253540814 1102 14-15 0.07692108047226043 1102 16-17 -0.08039924231428586 1102 18-19 -0.10675799240405581 1102 20-21 -0.035949962064940166 1102 22-23 -0.19344800110280858 1102 24-25 -0.0868318255495737 1102 26-27 -0.20641094440742336 1102 28-29 -0.11788046683933118 1102 30-31 -0.1523085085179119 1102 32-33 -0.17495375914064226 1102 34-35 -0.06874305778377732 1102 36-37 -0.13296648209679773 1102 38-39 0.04445026416238562 1102 40-41 -0.10696935472088853 1102 42-43 -0.1505802132285865 1102 44-45 -0.12304455487522858 1102 46-47 -0.2148139490611669 1102 48-49 -0.3463483606272817 1102 50-51 -0.44094945836777555 1102 52-53 -0.2569230265186846 1102 54-55 -0.2320296208905006 1102 56-57 -0.35108212033461683 1102 58-59 -0.41060282009428306 1102 60-61 -0.09697964973972262 1102 62-63 -0.183477318144611 1102 64-65 -0.2161713593668395 1102 66-67 -0.4756560158394265 1102 68-69 -0.479730044436252 1102 70-71 -0.5606291455906138 1102 72-73 -0.38177287899102197 1102 74-75 -0.49423566325923574 1102 76-77 -0.40409498926884524 1102 78-79 -0.3702251994638388 1102 80-81 -0.24189646473725546 1102 82-83 -0.359765534653981 1102 84-85 -0.3355106729844337 1102 86-87 -0.4479758413090984 1102 88-89 -0.5257390812760505 1102 90-91 -0.42113259845780604 1102 92-93 -0.4073424726235615 1102 94-95 -0.42162817414783405 1102 96-97 -0.34288925377328283 1102 98-99 -0.349776263659173 1102 100-101 0.02481628679540293 1103 1 0.022676478758246077 1103 2 -0.08894099703984182 1103 3 -0.09696354422089115 1103 4 -0.003487969565249216 1103 5 -0.12609690688154984 1103 6 -0.13177279985006152 1103 7 -0.07170489659547741 1103 8 -0.07079359444402655 1103 9 -0.02602607940091417 1103 10-11 -0.0907087233922752 1103 12-13 -0.09231268445947904 1103 14-15 -0.22189974207590524 1103 16-17 -0.15086228516732803 1103 18-19 -0.16657967776500016 1103 20-21 -0.26330112686218143 1103 22-23 -0.26951932695843794 1103 24-25 -0.2808208965878265 1103 26-27 -0.20367870397026167 1103 28-29 -0.32639355283300375 1103 30-31 -0.4032432223516693 1103 32-33 -0.2836681681138984 1103 34-35 -0.21872867757094383 1103 36-37 -0.19523280363835482 1103 38-39 -0.27047639675219415 1103 40-41 -0.11991154626531397 1103 42-43 -0.0812675874017188 1103 44-45 -0.2540482937305697 1103 46-47 -0.11932933588889938 1103 48-49 -0.06478379347169039 1103 50-51 -0.15722785928810623 1103 52-53 -0.08177203428400048 1103 54-55 -0.04968852224224207 1103 56-57 -0.11006975335158131 1103 58-59 -0.14859534238361505 1103 60-61 -0.07699115390998656 1103 62-63 -0.03406690687052816 1103 64-65 -0.08718085030730549 1103 66-67 0.05224159704524567 1103 68-69 -0.01294833606696244 1103 70-71 0.07871511670446552 1103 72-73 -0.0071683348437687755 1103 74-75 -0.018250618680578867 1103 76-77 0.024579155108487072 1103 78-79 -0.07284239819837879 1103 80-81 -0.1609358665204006 1103 82-83 -0.03376610986249062 1103 84-85 0.07921466495056606 1103 86-87 0.1437699165281181 1103 88-89 0.21512948357870698 1103 90-91 0.2629341257297142 1103 92-93 0.19073057885760036 1103 94-95 0.030341915009486797 1103 96-97 -0.10748516979283806 1103 98-99 0.10118721060024427 1103 100-101 0.5337320187482355 1104 1 -0.06008749919061529 1104 2 -0.07037832334054883 1104 3 -0.06418454529564599 1104 4 -0.15368343941621276 1104 5 -0.06333387036221438 1104 6 -0.14540578379474312 1104 7 -0.06447659081620571 1104 8 -0.04158340206462441 1104 9 -0.09386223813184813 1104 10-11 0.007186146531488191 1104 12-13 0.014326266719024261 1104 14-15 -0.03745496739178833 1104 16-17 -0.028789804458618562 1104 18-19 0.0139050549343267 1104 20-21 0.03353887373914688 1104 22-23 -0.2013648812546407 1104 24-25 -0.0760833866097812 1104 26-27 -0.031522665229701374 1104 28-29 -0.012874723379859176 1104 30-31 -0.06551486715967769 1104 32-33 -0.12642072568465323 1104 34-35 -0.03496915021786151 1104 36-37 -0.08719292152898817 1104 38-39 -0.01930299849473016 1104 40-41 0.04148003444661441 1104 42-43 -0.004760912000953965 1104 44-45 0.022782755896820106 1104 46-47 -0.05786446168433912 1104 48-49 0.060056539340898496 1104 50-51 -0.1054401166512875 1104 52-53 -0.0912152871546823 1104 54-55 -0.11028799597820438 1104 56-57 -0.09137301220108895 1104 58-59 -0.04512977270415064 1104 60-61 -0.09017658915725235 1104 62-63 -0.08945734258727356 1104 64-65 -0.06182497392637032 1104 66-67 -0.08116192454195215 1104 68-69 -0.13592306114859554 1104 70-71 -0.06526124996409521 1104 72-73 -0.013022326024398012 1104 74-75 0.06586869740783641 1104 76-77 0.03566276267629931 1104 78-79 0.14638524020100263 1104 80-81 0.20935042417085725 1104 82-83 0.20750588958349425 1104 84-85 0.20529832550459304 1104 86-87 0.2259521551045971 1104 88-89 0.1456687427919654 1104 90-91 0.3072495795418746 1104 92-93 0.2504302726958443 1104 94-95 0.4003561276379983 1104 96-97 0.6541898502125392 1104 98-99 0.5868172253452002 1104 100-101 0.9119749908659038 1105 1 0.018440125745037506 1105 2 -0.0508101115566042 1105 3 -0.06733280666018615 1105 4 -0.04835242060783429 1105 5 0.006514531527052725 1105 6 -0.03877468617139357 1105 7 -0.024964425237648413 1105 8 0.017774259970536832 1105 9 0.0029205885513192698 1105 10-11 -0.11783994098014006 1105 12-13 -0.005063895240432714 1105 14-15 0.00556724730085989 1105 16-17 -0.0163543480315127 1105 18-19 0.03435038001131829 1105 20-21 0.057801773655590694 1105 22-23 0.09666278010121943 1105 24-25 0.016853629338996257 1105 26-27 -0.023021614772567034 1105 28-29 -0.023914058027592944 1105 30-31 -0.016190564402130292 1105 32-33 -0.010610588752840044 1105 34-35 0.053130770476109035 1105 36-37 -0.02629513465025468 1105 38-39 0.017150810445663467 1105 40-41 -0.02357182548043113 1105 42-43 0.00865658066793884 1105 44-45 0.029588425009777097 1105 46-47 -0.020249216265298742 1105 48-49 0.0803374824034293 1105 50-51 -0.06355527654420001 1105 52-53 -0.14272345223891136 1105 54-55 -0.040066552902992214 1105 56-57 0.010177915941426363 1105 58-59 -0.01610932909778029 1105 60-61 0.09160831225037214 1105 62-63 0.16781364589002834 1105 64-65 0.1606405680514058 1105 66-67 -0.0010725681648651175 1105 68-69 -0.07126314818062696 1105 70-71 -0.08508378941046146 1105 72-73 0.008997702637081773 1105 74-75 0.020694252775154354 1105 76-77 -0.069690803926445 1105 78-79 -0.11619593799695593 1105 80-81 0.10423056075074655 1105 82-83 0.17094392488900212 1105 84-85 0.29030522897630107 1105 86-87 0.36532985234869386 1105 88-89 0.2964885249418039 1105 90-91 0.47040701552618813 1105 92-93 0.6159706954485742 1105 94-95 0.640011887064901 1105 96-97 0.6877108090216879 1105 98-99 0.8743901578434077 1105 100-101 0.9771891867280758 1106 1 -0.15049880853114672 1106 2 -0.06159987337501249 1106 3 -0.04223169507734781 1106 4 0.1291893776227795 1106 5 0.034385060573100645 1106 6 -0.10393764211888623 1106 7 -0.3861520157997589 1106 8 -0.30598253594821756 1106 9 -0.4421216350956456 1106 10-11 -0.6379290463682352 1106 12-13 -0.23687807681672268 1106 14-15 -0.34606222623136773 1106 16-17 -0.29626111739397487 1106 18-19 -0.4946255440015861 1106 20-21 -0.4568045314444191 1106 22-23 -0.4287068164665726 1106 24-25 -0.4590527653133023 1106 26-27 -0.5989773405328549 1106 28-29 -0.287457681681083 1106 30-31 -0.32749383383420394 1106 32-33 -0.12655373250230895 1106 34-35 -0.08096149416552834 1106 36-37 -0.3024664906369381 1106 38-39 -0.20269249882944962 1106 40-41 -0.1803440240493117 1106 42-43 -0.09152218014150293 1106 44-45 -0.0812269477322971 1106 46-47 -0.23531187708557155 1106 48-49 0.029035708374273383 1106 50-51 -0.01793285375082121 1106 52-53 0.09666975794426946 1106 54-55 -0.025809518922976338 1106 56-57 -0.03892638927202796 1106 58-59 -0.03810885469409442 1106 60-61 -0.23866887013549842 1106 62-63 -0.1323555752800658 1106 64-65 -0.05684619247614364 1106 66-67 0.031315023084793836 1106 68-69 -0.05682786904733916 1106 70-71 0.0861356343265669 1106 72-73 -0.024749072363221103 1106 74-75 0.15373689926018486 1106 76-77 0.27998519210643735 1106 78-79 0.24564662185783348 1106 80-81 -0.11421695318486158 1106 82-83 0.0033310004824791406 1106 84-85 -0.08373277144653812 1106 86-87 0.15256237719043142 1106 88-89 0.4890126275711353 1106 90-91 0.5716071193449714 1106 92-93 0.5943094403070539 1106 94-95 0.6232542643774011 1106 96-97 0.8597992853092364 1106 98-99 0.7045881870054842 1106 100-101 0.8809663682545192 1107 1 0.02008580962875328 1107 2 -0.025800708625624225 1107 3 -0.05220058435438091 1107 4 -0.008120869158410926 1107 5 0.037714824214646114 1107 6 0.02291264414844818 1107 7 0.08912834578902107 1107 8 0.05370781715061668 1107 9 -0.03747696737048045 1107 10-11 -0.05696322879189353 1107 12-13 -0.02283141717490622 1107 14-15 0.04729719854238823 1107 16-17 0.06353124394282617 1107 18-19 -0.0021023899211840558 1107 20-21 0.07915480413475962 1107 22-23 0.07261982751250429 1107 24-25 0.0332048989056446 1107 26-27 -0.040496107185255426 1107 28-29 0.03455789730335823 1107 30-31 0.04834200735077587 1107 32-33 0.18160241669743726 1107 34-35 0.10613628914178008 1107 36-37 0.016109263186045553 1107 38-39 0.10437744159774098 1107 40-41 0.18551567990488138 1107 42-43 0.22114845995482568 1107 44-45 0.13603986143570523 1107 46-47 0.1746237073965773 1107 48-49 0.06251892819809513 1107 50-51 0.06894792045233089 1107 52-53 -0.030458098107438047 1107 54-55 0.0032630078604540813 1107 56-57 0.07952897693396466 1107 58-59 0.08843827024845297 1107 60-61 0.22531935847176499 1107 62-63 0.17274667992521842 1107 64-65 0.26211477666654304 1107 66-67 0.09443924191644726 1107 68-69 0.04393703167180263 1107 70-71 0.16297647140974902 1107 72-73 0.22340735243773935 1107 74-75 0.2572289991695591 1107 76-77 0.25048951009725684 1107 78-79 0.4220052642105081 1107 80-81 0.5131885621321217 1107 82-83 0.49437431926502384 1107 84-85 0.5561455774660189 1107 86-87 0.46334899671616725 1107 88-89 0.46967598177380054 1107 90-91 0.6081689298945534 1107 92-93 0.6637432265808272 1107 94-95 0.872215301525582 1107 96-97 0.7347044355441064 1107 98-99 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1.2338686775349572 2310 86-87 1.2160409018542389 2310 88-89 1.1302465844672014 2310 90-91 1.2833845180355574 2310 92-93 1.411824646659987 2310 94-95 1.5609830048452906 2310 96-97 1.5893803225697383 2310 98-99 1.5620515055796957 2310 100-101 1.742080975886111 2311 1 0.25641935534739346 2311 2 0.28154255179218524 2311 3 0.2068125490796433 2311 4 0.1596308652787073 2311 5 0.2280443248417754 2311 6 0.19534199775146277 2311 7 0.30178260154308845 2311 8 0.2731725444771982 2311 9 0.3539614236642876 2311 10-11 0.3537864437593399 2311 12-13 0.3703858614751283 2311 14-15 0.4095258287497501 2311 16-17 0.46451675288846417 2311 18-19 0.42473402299904706 2311 20-21 0.4795855014733661 2311 22-23 0.47197445645790026 2311 24-25 0.4778781096623135 2311 26-27 0.5937975175530568 2311 28-29 0.6197984236589278 2311 30-31 0.5575449955039957 2311 32-33 0.48074248763310123 2311 34-35 0.5336613472648963 2311 36-37 0.5264887913512695 2311 38-39 0.55948832292475 2311 40-41 0.5600169858430846 2311 42-43 0.5902943007769252 2311 44-45 0.5983535550423937 2311 46-47 0.44658177004149735 2311 48-49 0.5097586006413266 2311 50-51 0.3441686108948332 2311 52-53 0.37961556050967715 2311 54-55 0.415469904571772 2311 56-57 0.5231296869076942 2311 58-59 0.46502209636197733 2311 60-61 0.4246535074057931 2311 62-63 0.4410542094727461 2311 64-65 0.4885872547710548 2311 66-67 0.6078938572856387 2311 68-69 0.5609206885456004 2311 70-71 0.848383055559836 2311 72-73 0.8576778359202777 2311 74-75 0.8645512974665088 2311 76-77 0.6439995256327542 2311 78-79 0.7024267590430675 2311 80-81 0.8534590332726602 2311 82-83 0.9336705628221154 2311 84-85 0.911808165614918 2311 86-87 1.0646212065363905 2311 88-89 1.2146275239842232 2311 90-91 1.1120394013436439 2311 92-93 1.3587802371625521 2311 94-95 1.2769715816767118 2311 96-97 1.3229233379389171 2311 98-99 1.4792841851038574 2311 100-101 1.510588149134282 2312 1 0.3819598279466838 2312 2 0.48876082371633345 2312 3 0.44321993254585124 2312 4 0.29059401977126953 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2312 68-69 1.040741847778527 2312 70-71 1.123384851149872 2312 72-73 1.2849277102289776 2312 74-75 1.2835306840929555 2312 76-77 1.3094559646186035 2312 78-79 1.3302799516021437 2312 80-81 1.4504101214002212 2312 82-83 1.3875346725685773 2312 84-85 1.2450660841669574 2312 86-87 1.3903304645985877 2312 88-89 1.4086122973807633 2312 90-91 1.4823654804932538 2312 92-93 1.766731109819304 2312 94-95 1.8011438146724714 2312 96-97 1.8041019632351905 2312 98-99 1.8892604833154536 2312 100-101 1.6836435251308366 2313 1 0.4347797253210395 2313 2 0.4565352934710063 2313 3 0.4657260293093728 2313 4 0.42225641678020764 2313 5 0.49844544381598865 2313 6 0.5725099666491928 2313 7 0.5713080673411284 2313 8 0.5011868132602473 2313 9 0.5992263509327103 2313 10-11 0.5548419403885063 2313 12-13 0.7126504693192359 2313 14-15 0.7997183386277555 2313 16-17 0.7485512972608106 2313 18-19 0.8156398556637612 2313 20-21 0.7550243899000222 2313 22-23 0.8911010491430247 2313 24-25 0.7705694020623994 2313 26-27 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2314 52-53 0.9569415212113981 2314 54-55 0.7483076051019921 2314 56-57 0.906021638124038 2314 58-59 0.9994420586162889 2314 60-61 0.7188003779005214 2314 62-63 0.7813646173120148 2314 64-65 0.9129020526198133 2314 66-67 0.931003400710658 2314 68-69 1.0064791758806138 2314 70-71 1.1860930771502183 2314 72-73 1.1087093982301397 2314 74-75 1.3192762025278135 2314 76-77 1.365543891803899 2314 78-79 1.5504076943728542 2314 80-81 1.5068501007922883 2314 82-83 1.4517468814484786 2314 84-85 1.6592974478573623 2314 86-87 1.675541629871578 2314 88-89 1.7186850856606597 2314 90-91 1.8865828413566703 2314 92-93 2.085860820911922 2314 94-95 2.0872123228429906 2314 96-97 2.0875422993030384 2314 98-99 2.2565434064716356 2314 100-101 2.04280811118878 2315 1 0.10621737554087929 2315 2 0.14781157104911102 2315 3 0.1749906995698396 2315 4 0.09543704169472988 2315 5 0.1721704131351629 2315 6 0.11445442921858984 2315 7 0.343780150696432 2315 8 0.3111882702083264 2315 9 0.26738452722700146 2315 10-11 0.22876534067292909 2315 12-13 0.3384994991080106 2315 14-15 0.4882684250167344 2315 16-17 0.28338486999246726 2315 18-19 0.4677586425176088 2315 20-21 0.3927730207710596 2315 22-23 0.502223283511448 2315 24-25 0.5255563208138554 2315 26-27 0.5696918551215546 2315 28-29 0.5021536115518259 2315 30-31 0.34043355387593266 2315 32-33 0.250114479039091 2315 34-35 0.35028820425626606 2315 36-37 0.48245507519884967 2315 38-39 0.439453004542564 2315 40-41 0.13990091405343463 2315 42-43 0.26906424279238905 2315 44-45 0.3690816002899808 2315 46-47 0.3851122506013027 2315 48-49 0.41345053345295213 2315 50-51 0.3588698393351848 2315 52-53 0.4553533340393727 2315 54-55 0.17789055536206888 2315 56-57 0.27599826234184377 2315 58-59 0.2908531086232742 2315 60-61 0.27920943106622076 2315 62-63 0.38997029718198206 2315 64-65 0.5092615393042124 2315 66-67 0.6769912999446248 2315 68-69 0.6117308223921327 2315 70-71 0.6820464422344941 2315 72-73 0.7577775901294999 2315 74-75 0.7912386938367213 2315 76-77 0.7697726368022053 2315 78-79 0.9189473142516285 2315 80-81 0.8824028390433831 2315 82-83 0.878126597470331 2315 84-85 0.7816446495935807 2315 86-87 1.0405986726125782 2315 88-89 0.9468272711965291 2315 90-91 1.1236359886455851 2315 92-93 1.141851421483807 2315 94-95 1.3032729279733815 2315 96-97 1.4109323556167226 2315 98-99 1.7050856049002547 2315 100-101 1.7284211076939222 2316 1 -0.011992841382866715 2316 2 -0.12168886060269557 2316 3 0.1092960395767264 2316 4 0.14202424783466938 2316 5 0.18293675726894065 2316 6 0.20070494695124097 2316 7 0.11421499622496611 2316 8 -0.10644857339399749 2316 9 0.03272937460191372 2316 10-11 -0.38483393674693644 2316 12-13 0.04917614449981755 2316 14-15 0.1404306761931764 2316 16-17 0.18566938092847352 2316 18-19 0.29092299955603096 2316 20-21 0.30006394410669657 2316 22-23 0.20130022232667244 2316 24-25 0.39242417547944086 2316 26-27 0.3908439163213018 2316 28-29 0.4313022095567476 2316 30-31 0.42056484588713516 2316 32-33 0.25917854315310507 2316 34-35 0.2690391087289683 2316 36-37 0.29980024194619403 2316 38-39 0.21177975747306022 2316 40-41 0.3290735647947258 2316 42-43 0.5395708223635225 2316 44-45 0.20677989813471243 2316 46-47 -0.021335953677287023 2316 48-49 0.1355166734877855 2316 50-51 0.0013710744082615633 2316 52-53 -0.1292186070962984 2316 54-55 -0.12581765799867384 2316 56-57 0.12841451440652918 2316 58-59 0.2731716027822486 2316 60-61 0.32077275912658365 2316 62-63 0.17218075363505392 2316 64-65 0.12825334540521993 2316 66-67 0.09598110996792997 2316 68-69 0.16651816526338337 2316 70-71 0.14148926209347223 2316 72-73 0.1245381556183176 2316 74-75 0.4254979993014807 2316 76-77 0.3781047641719262 2316 78-79 0.6134382645343237 2316 80-81 0.6421605401606918 2316 82-83 0.4338764240610793 2316 84-85 0.38362425965980407 2316 86-87 0.2553242368787956 2316 88-89 0.34029098998910356 2316 90-91 0.4029115870946356 2316 92-93 0.4515590959035727 2316 94-95 0.7476852826963523 2316 96-97 0.965740283039338 2316 98-99 0.9451875545691557 2316 100-101 0.5434682899807264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11141.0 3 2490.0 4 1826.0 5 1996.0 6 2202.0 7 2599.0 8 3124.0 9 4046.0 10 5106.0 11 6581.0 12 8705.0 13 11232.0 14 12976.0 15 15791.0 16 19633.0 17 24443.0 18 21794.0 19 16432.0 20 16156.0 21 17267.0 22 18766.0 23 21155.0 24 23577.0 25 26814.0 26 31510.0 27 37370.0 28 44787.0 29 54372.0 30 67598.0 31 85518.0 32 109141.0 33 144834.0 34 198214.0 35 283467.0 36 412836.0 37 560263.0 38 422313.0 39 51769.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98080500189233 19.002758101632626 14.846306460431464 23.170130436043586 2 15.178489610776385 19.774138908616067 40.63076304093835 24.416608439669194 3 19.04626747718624 23.965327667713698 29.726965047059196 27.261439808040866 4 12.619648437080548 16.30110852205411 37.519373185721285 33.55986985514405 5 13.646459168899316 37.1972588961491 34.58250783440984 14.573774100541742 6 32.14819074230355 36.274056630627 17.348737406196552 14.229015220872906 7 28.58921304448389 32.40916645601844 20.73183132197098 18.26978917752669 8 26.28646323165879 34.928483180399674 19.81574211342378 18.96931147451776 9 25.964118047585327 16.654005789397942 20.413462963533092 36.968413199483635 10-11 24.690126620164925 26.6501489175152 27.52424944247674 21.13547501984314 12-13 25.514569780638784 23.7912398724003 28.10310012853056 22.591090218430352 14-15 22.553508102933016 25.271990536689216 26.23647072489583 25.93803063548194 16-17 21.990359555602257 28.50302089574468 26.957442165657614 22.54917738299545 18-19 21.988709832161764 27.118759048100454 29.28632951051835 21.606201609219433 20-21 22.57391257657045 26.255898553383417 29.520804342087008 21.64938452795912 22-23 22.24199714997632 26.050548008509267 29.454003187931534 22.25345165358288 24-25 22.127157020323654 26.239045662854732 29.544992892331575 22.08880442449004 26-27 21.847791632532925 26.556646383601056 29.415182247792277 22.180379736073746 28-29 21.870437505415026 26.80108221745024 29.316016947150068 22.01246332998467 30-31 22.000994219243424 26.482731929743032 29.279416600597212 22.23685725041633 32-33 21.88782374645473 26.42793569142884 29.26382968668439 22.420410875432037 34-35 22.300701624233604 26.59632402751501 29.117906957093364 21.985067391158015 36-37 22.28452842885984 26.327497458512905 29.135339556993745 22.25263455563351 38-39 21.987874340583375 26.632189822500035 29.147078431986685 22.23285740492991 40-41 22.300640638791826 26.510662637707988 29.004511460125265 22.18418526337492 42-43 22.02357088447374 26.46007898007457 28.97726459184082 22.539085543610863 44-45 22.155341317869077 26.652124918213854 28.99516040825496 22.19737335566211 46-47 22.433618472415244 26.84045930408353 28.376712271136995 22.349209952364234 48-49 22.246418740278823 26.91947572248209 28.35615094426096 22.477954592978126 50-51 22.395702417551256 27.123243624916128 28.230795617055986 22.250258340476638 52-53 22.515995380853102 27.076910514862263 28.05950885124596 22.34758525303868 54-55 22.307036235119554 27.119014566876835 28.140303626089974 22.433645571913637 56-57 22.25991228454814 27.330030058784203 28.017041961490037 22.393015695177617 58-59 22.327543892330322 27.03106878918135 28.09760503997923 22.5437822785091 60-61 22.26260771037545 27.133763250295345 28.199083596171043 22.40454544315816 62-63 22.17142722160672 27.143726090673308 28.398549115351557 22.28629757236841 64-65 22.093086509356105 27.087614495361496 28.256939866538687 22.562359128743715 66-67 22.040626210093315 27.180100536610404 28.097606260955704 22.68166699234058 68-69 22.179749592621846 27.034251259892766 28.285679722929167 22.500319424556213 70-71 22.16431188833639 27.001376665334682 28.14096852602681 22.69334292030212 72-73 22.158653998436307 27.09216654763283 28.002234054223234 22.746945399707627 74-75 22.112007101108475 27.341307763616125 27.87492886364368 22.671756271631715 76-77 22.286878487129314 27.082505168553645 27.932401350025675 22.69821499429137 78-79 22.252692824295508 27.124340160732775 27.946688477119157 22.67627853785256 80-81 22.279210987561218 27.20976920265877 27.77886147900282 22.732158330777192 82-83 22.29246166984131 27.197501515740434 27.82018987735924 22.689846937059013 84-85 22.27824705735399 27.23864611261493 27.773810942666767 22.709295887364313 86-87 22.281627678480348 27.197414269837267 27.809663915927974 22.71129413575441 88-89 22.319989047538517 27.096908703538165 27.717834526561063 22.86526772236226 90-91 22.26325233009231 27.069323322400656 27.78730049353659 22.880123853970442 92-93 22.275278028471533 26.892564855712248 27.93640533252014 22.895751783296085 94-95 22.185985524926757 26.933808116785563 27.912234735057627 22.967971623230056 96-97 22.28708225770195 26.835231169470823 27.819496525764325 23.058190047062897 98-99 22.069590726486663 26.925871136968315 27.767668171026266 23.236869965518753 100-101 22.44793405762013 26.43979647820107 27.999278504700992 23.112990959477802 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3836.0 1 2256.5 2 658.0 3 594.0 4 483.5 5 457.5 6 714.0 7 1452.5 8 2388.0 9 2830.5 10 2603.5 11 2337.0 12 2441.5 13 2649.0 14 2859.5 15 3017.5 16 3159.5 17 3364.5 18 3640.0 19 3998.5 20 4533.0 21 5387.0 22 6477.5 23 7996.0 24 10009.5 25 12868.5 26 17111.5 27 22107.0 28 26269.5 29 30315.0 30 34766.0 31 40341.0 32 47281.0 33 51546.0 34 55465.0 35 60912.5 36 67156.5 37 72914.0 38 76445.5 39 78897.5 40 82091.5 41 86478.0 42 89355.0 43 92634.0 44 96996.0 45 100610.5 46 102058.5 47 103419.5 48 104864.0 49 104027.5 50 101441.5 51 97917.5 52 96087.5 53 95377.5 54 93808.0 55 87127.0 56 78900.0 57 72893.5 58 67329.5 59 59792.5 60 48810.5 61 39956.5 62 32587.5 63 24292.5 64 17412.5 65 12978.0 66 9725.0 67 7298.0 68 5583.0 69 4319.5 70 3243.5 71 2232.0 72 1555.0 73 1153.0 74 872.5 75 609.5 76 432.0 77 284.5 78 188.5 79 134.0 80 88.5 81 53.5 82 42.0 83 36.0 84 25.0 85 22.0 86 17.5 87 15.0 88 12.0 89 8.0 90 9.0 91 6.5 92 4.0 93 3.0 94 4.5 95 6.0 96 2.5 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2497628449463115 2 0.221154230789012 3 0.21172517443754252 4 0.21593967689766902 5 0.21754690241212404 6 0.24697698738792281 7 0.24608408432433668 8 0.23561926041910727 9 0.23122617734626355 10-11 0.23926230491853867 12-13 0.2409409626780806 14-15 0.22610091376127914 16-17 0.24028021441102684 18-19 0.23044042265030776 20-21 0.22388651416358554 22-23 0.22126137915664235 24-25 0.21581467046876696 26-27 0.22538659131041025 28-29 0.23936945328616901 30-31 0.23963732420524483 32-33 0.23922658879599523 34-35 0.2467269745301187 36-37 0.22201141773005467 38-39 0.22120780497282716 40-41 0.21722545730923304 42-43 0.22286860467109737 44-45 0.2410302529844392 46-47 0.24613765850815184 48-49 0.2494413998434205 50-51 0.251084341480419 52-53 0.27021032510243387 54-55 0.25047716739718046 56-57 0.22013632129652383 58-59 0.2042069306421473 60-61 0.20261756318896398 62-63 0.20845714922481726 64-65 0.20136749889994343 66-67 0.20179609237046475 68-69 0.20604631095313472 70-71 0.2066356269751016 72-73 0.20831428473464347 74-75 0.21165374219245559 76-77 0.22427939151156345 78-79 0.21745761210576545 80-81 0.21190375505025974 82-83 0.2086535878988062 84-85 0.20918932973695792 86-87 0.2164754187358207 88-89 0.21299309678783482 90-91 0.22299361109999943 92-93 0.21224305821442246 94-95 0.21002865861672887 96-97 0.21958272139710042 98-99 0.22531515906532334 100-101 0.20211753747335576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2799856.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.498955178027 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69391092289858 37.43660460333841 2 14.601059911094039 15.038786598262377 3 5.267803787675014 8.13859173444349 4 2.425477505236866 4.996382293100245 5 1.3690844632836592 3.5253209704789166 6 0.8263909926728051 2.553496361470922 7 0.5423109454141035 1.9549912949304056 8 0.38109834732079023 1.5700933365674834 9 0.29289526645579916 1.357542017915713 >10 1.451052766394413 13.541405162700881 >50 0.10044297076190381 3.532128741146746 >100 0.04501174759141429 4.1345714665233 >500 0.0026298836325687504 0.9026470272210856 >1k 6.22867176112786E-4 0.5384660541290831 >5k 2.076223920375953E-4 0.7789723377710644 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9687 0.3459820790783526 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7095 0.25340588944574294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5074 0.18122360578544042 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4098 0.146364670183038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.571612254344509E-5 6 0.0 0.0 0.0 0.0 3.571612254344509E-5 7 0.0 0.0 0.0 0.0 3.571612254344509E-5 8 0.0 0.0 0.0 0.0 3.571612254344509E-5 9 0.0 0.0 0.0 0.0 3.571612254344509E-5 10-11 0.0 0.0 0.0 0.0 7.143224508689018E-5 12-13 0.0 0.0 0.0 1.7858061271722546E-5 2.678709190758382E-4 14-15 0.0 0.0 0.0 3.571612254344509E-5 3.035870416192833E-4 16-17 0.0 0.0 0.0 5.3574183815167635E-5 3.2144510289100584E-4 18-19 0.0 0.0 0.0 7.143224508689018E-5 3.2144510289100584E-4 20-21 0.0 0.0 0.0 7.143224508689018E-5 3.5716122543445094E-4 22-23 0.0 0.0 0.0 7.143224508689018E-5 3.92877347977896E-4 24-25 0.0 0.0 0.0 1.2500642890205782E-4 3.92877347977896E-4 26-27 0.0 0.0 0.0 2.1429673526067054E-4 4.285934705213411E-4 28-29 0.0 0.0 0.0 6.071740832385666E-4 4.285934705213411E-4 30-31 0.0 0.0 0.0 0.0017322319433570868 4.285934705213411E-4 32-33 0.0 0.0 0.0 0.0038930573572355147 4.285934705213411E-4 34-35 0.0 0.0 0.0 0.006839637467069735 4.285934705213411E-4 36-37 0.0 0.0 0.0 0.011625597887891376 4.285934705213411E-4 38-39 0.0 0.0 0.0 0.01830451280351561 4.643095930647862E-4 40-41 0.0 0.0 0.0 0.026751375785040374 4.643095930647862E-4 42-43 0.0 0.0 0.0 0.03862698653073587 4.643095930647862E-4 44-45 0.0 0.0 0.0 0.05498497065563372 4.8216765433650873E-4 46-47 0.0 0.0 0.0 0.08159348195050031 5.000257156082313E-4 48-49 0.0 0.0 0.0 0.11886325582458526 5.000257156082313E-4 50-51 0.0 0.0 0.0 0.15632946837265915 5.000257156082313E-4 52-53 0.0 0.0 0.0 0.19574220959935082 5.535998994233989E-4 54-55 0.0 0.0 0.0 0.2434768073786652 5.714579606951215E-4 56-57 0.0 0.0 0.0 0.29642595904932256 5.89316021966844E-4 58-59 0.0 0.0 0.0 0.36107214085295813 6.071740832385666E-4 60-61 0.0 0.0 0.0 0.43975475881616766 6.071740832385666E-4 62-63 0.0 0.0 0.0 0.5219018406660914 6.071740832385666E-4 64-65 0.0 0.0 0.0 0.6124779274362682 6.071740832385666E-4 66-67 0.0 0.0 0.0 0.7310375962192341 6.250321445102891E-4 68-69 0.0 0.0 0.0 0.8700447451583224 6.428902057820117E-4 70-71 0.0 0.0 0.0 1.0256777491413844 6.786063283254568E-4 72-73 0.0 0.0 0.0 1.1852216685429537 8.036127572275145E-4 74-75 0.0 0.0 0.0 1.3494443999977142 8.214708184992371E-4 76-77 0.0 0.0 0.0 1.5318644958883598 8.214708184992371E-4 78-79 0.0 0.0 0.0 1.7456433473721504 8.214708184992371E-4 80-81 0.0 0.0 0.0 1.9812983239138013 8.393288797709596E-4 82-83 0.0 0.0 0.0 2.2493478236023567 8.929030635861273E-4 84-85 0.0 0.0 0.0 2.5313623272053993 8.929030635861273E-4 86-87 0.0 0.0 0.0 2.864593750535742 9.286191861295724E-4 88-89 0.0 0.0 0.0 3.257453240452366 9.643353086730175E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5500 0.0 44.6633 1 GTATCAA 9175 0.0 41.1703 1 ATCAACG 11560 0.0 32.21778 3 TCAACGC 11785 0.0 31.56802 4 TATCAAC 11860 0.0 31.443445 2 CAACGCA 12040 0.0 30.858307 5 AACGCAG 12500 0.0 29.721659 6 ACGCAGA 14355 0.0 25.649256 7 CGCAGAG 14395 0.0 25.379961 8 GTACATG 9030 0.0 23.099348 1 GCAGAGT 15710 0.0 22.469267 9 TGGTATC 2815 0.0 22.444937 2 TACATGG 9150 0.0 22.1693 2 GAGTACT 9360 0.0 21.697678 12-13 GGACCGA 740 0.0 21.186464 6 ACATGGG 9145 0.0 20.674583 3 AGGACCG 810 0.0 20.529333 5 GTGGTAT 3270 0.0 20.487751 1 AGAGTAC 13920 0.0 20.476753 10-11 TAAGGTG 1275 0.0 20.122198 5 >>END_MODULE