FastQCFastQC Report
Thu 26 May 2016
SRR937160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799856
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101950.3641258693304227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83810.2993368230366133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58020.2072249429970684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59350.048.852031
GTATCAA98600.038.853631
TATCAAC125950.030.1691252
TCAACGC126100.030.0202394
ATCAACG126200.029.9588153
CAACGCA129300.029.3507485
AACGCAG132950.028.580686
ACGCAGA156200.024.2074767
CGCAGAG156000.024.147168
GAGTACT105300.021.56338712-13
GCAGAGT172300.021.3940939
GTACATG93450.021.1077671
TACATGG90900.020.744242
GTACTTT110600.020.35607714-15
AGTACTT110650.019.8124312-13
AGAGTAC153050.019.80180510-11
CAGAGTA162750.019.64316710-11
ACATGGG91700.019.6309283
TGGTATC29300.019.1286932
GTGGTAT31650.018.4716151
Produced by FastQC (version 0.11.5)