FastQCFastQC Report
Thu 26 May 2016
SRR937159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762865
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111131.45674529569452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76571.0037162538588087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61060.8004037411599694No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.22992272551499937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.22271306194411852No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16120.2113086850229071No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12770.16739527963663295No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11830.15507330917003664No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11590.15192727415728863No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.1245305525879415No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9340.12243319591277617No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8430.11050447982277337No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8300.10880037752420152No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8190.10735844481002535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47550.039.5915451
GTATCAA78500.037.7896351
CCGACTA753.0754032E-431.6703059
TATCAAC100600.029.564942
ATCAACG100800.029.5062773
TCAACGC101700.029.2470844
CAACGCA102550.029.0084825
AACGCAG104300.028.510516
GTACATG62800.027.4035421
TACATGG62400.027.2583182
GTGTACG700.00835556827.1656271
ACATGGG63400.025.3295843
ACGCAGA117300.025.2714447
CGCAGAG117850.025.1131958
GAGTACT66300.024.5344512-13
TAAGGTG3100.024.5270125
GCAGAGT120000.024.306969
AGTACTT67150.024.29461512-13
GTGTAAG2853.6379788E-1223.3529071
CATGGGA43850.023.080184
Produced by FastQC (version 0.11.5)