##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937159_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 762865 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30335118271254 33.0 31.0 34.0 31.0 34.0 2 32.41904662030635 34.0 31.0 34.0 31.0 34.0 3 32.44043834754511 34.0 31.0 34.0 31.0 34.0 4 36.03554888479613 37.0 35.0 37.0 35.0 37.0 5 36.05579624179901 37.0 37.0 37.0 35.0 37.0 6 36.14078768851632 37.0 37.0 37.0 35.0 37.0 7 36.10430547999974 37.0 37.0 37.0 35.0 37.0 8 36.10118697279335 37.0 37.0 37.0 35.0 37.0 9 37.79645939976274 39.0 38.0 39.0 35.0 39.0 10-11 37.71303900427992 39.0 38.0 39.0 35.0 39.0 12-13 37.59380624356865 39.0 38.0 39.0 35.0 39.0 14-15 38.76841315304805 40.0 38.0 41.0 35.5 41.0 16-17 38.73940998735032 40.0 38.0 41.0 36.0 41.0 18-19 38.753571077451454 40.0 38.0 41.0 36.0 41.0 20-21 38.691518813944796 40.0 38.0 41.0 35.0 41.0 22-23 38.73502389020338 40.0 38.0 41.0 35.0 41.0 24-25 38.694217849816155 40.0 38.0 41.0 35.0 41.0 26-27 38.59502402128817 40.0 38.0 41.0 35.0 41.0 28-29 38.44664193533587 40.0 38.0 41.0 35.0 41.0 30-31 38.11471557877213 40.0 37.5 41.0 34.5 41.0 32-33 37.77615371002733 40.0 37.0 41.0 34.0 41.0 34-35 37.72882751207619 40.0 37.0 41.0 34.0 41.0 36-37 37.580311064211884 40.0 37.0 41.0 33.5 41.0 38-39 37.41445537545962 40.0 36.5 41.0 33.0 41.0 40-41 37.150266429840144 40.0 36.0 41.0 33.0 41.0 42-43 36.79654591572559 40.0 36.0 41.0 32.5 41.0 44-45 36.39186946576393 39.0 35.0 41.0 31.0 41.0 46-47 35.77598067810163 39.0 35.0 41.0 29.0 41.0 48-49 35.25857261769775 39.0 35.0 40.5 26.5 41.0 50-51 34.225944957495756 38.0 34.0 39.5 23.5 40.5 52-53 34.299042425592994 38.0 34.5 39.5 24.0 40.5 54-55 34.576058018129025 38.5 35.0 40.0 23.5 41.0 56-57 34.32477961369312 38.0 35.0 40.0 22.5 41.0 58-59 34.060192170305356 38.0 34.0 40.0 20.5 41.0 60-61 33.73239498469585 38.0 34.0 40.0 19.0 41.0 62-63 33.36738938082098 37.0 34.0 40.0 17.5 41.0 64-65 32.96283746141191 36.5 33.0 39.5 11.5 41.0 66-67 32.52973592968611 36.0 33.0 39.0 7.5 41.0 68-69 32.04930426746541 35.5 32.0 39.0 3.5 40.0 70-71 31.588360981300752 35.0 31.5 38.5 2.0 40.0 72-73 31.122916243372025 35.0 31.0 37.5 2.0 40.0 74-75 30.603448185458763 35.0 31.0 37.0 2.0 39.0 76-77 30.118958793495572 35.0 30.0 36.5 2.0 39.0 78-79 29.60485079273528 34.0 29.5 36.0 2.0 38.5 80-81 29.06054413297241 34.0 29.0 35.5 2.0 37.0 82-83 28.537128456542113 34.0 29.0 35.0 2.0 37.0 84-85 28.006840004456883 34.0 27.5 35.0 2.0 36.0 86-87 27.457356806250125 33.5 26.0 35.0 2.0 36.0 88-89 26.93060436643443 33.0 25.5 35.0 2.0 35.5 90-91 26.402380499826315 33.0 24.5 35.0 2.0 35.0 92-93 25.773223309497748 32.5 22.5 35.0 2.0 35.0 94-95 25.037683600637074 32.0 19.0 34.5 2.0 35.0 96-97 24.255001212534328 31.5 6.5 34.0 2.0 35.0 98-99 23.1544808059093 31.0 2.0 34.0 2.0 35.0 100-101 20.107325018188014 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13029873308008177 1101 2 0.037055939238328506 1101 3 0.013534315782230522 1101 4 -0.008162913156944285 1101 5 -0.055756269899276845 1101 6 2.3111711924883593E-4 1101 7 -0.0547530566657457 1101 8 -0.030129747935575324 1101 9 0.08703965267267932 1101 10-11 0.10897151290135554 1101 12-13 0.10755100332428214 1101 14-15 0.2325934709134927 1101 16-17 0.2462838639933551 1101 18-19 0.15540195136368595 1101 20-21 0.19796581274159308 1101 22-23 0.1869112210202104 1101 24-25 0.292588361618904 1101 26-27 0.22431982555853125 1101 28-29 0.10272332552455055 1101 30-31 0.2049279032981488 1101 32-33 0.195403040836176 1101 34-35 0.4578400589344156 1101 36-37 0.25905596340359693 1101 38-39 0.29571805618644476 1101 40-41 0.2813058853437198 1101 42-43 0.3781894602421474 1101 44-45 0.24934554221076866 1101 46-47 0.45157775328660676 1101 48-49 0.5295935267388145 1101 50-51 0.5480066297002182 1101 52-53 0.46522886675534636 1101 54-55 0.6303272035375613 1101 56-57 0.4461278728434408 1101 58-59 0.5444502800384399 1101 60-61 0.6529647164817334 1101 62-63 0.663303325050066 1101 64-65 0.7282089932147855 1101 66-67 0.94253541729163 1101 68-69 0.8334422891027486 1101 70-71 0.6382044339443915 1101 72-73 0.7520092427152001 1101 74-75 0.7908253371276466 1101 76-77 0.7390676437720387 1101 78-79 0.9145876757875904 1101 80-81 0.84875963988301 1101 82-83 0.7513192716624921 1101 84-85 0.9889708148415401 1101 86-87 1.0053459607183228 1101 88-89 1.0649427105779843 1101 90-91 1.2699460698664673 1101 92-93 1.341216574849934 1101 94-95 1.5234575273083237 1101 96-97 1.361423428286468 1101 98-99 1.5678408365837697 1101 100-101 1.4858125138845502 1102 1 0.06742757487436535 1102 2 0.0320523744118546 1102 3 0.05821205119952566 1102 4 0.127289819805533 1102 5 0.02598427619513899 1102 6 -0.01892929153367362 1102 7 -0.07677955734384767 1102 8 -0.04554744455959536 1102 9 0.038215049250730715 1102 10-11 0.04453567337404962 1102 12-13 0.04434257411063669 1102 14-15 0.19727500596619763 1102 16-17 0.3042838042238074 1102 18-19 0.20792052617309764 1102 20-21 0.10295358133099342 1102 22-23 0.11365652733201159 1102 24-25 0.215528695958362 1102 26-27 0.0899059442238439 1102 28-29 0.009297833244076514 1102 30-31 0.11807279179237185 1102 32-33 0.21553399306466048 1102 34-35 0.2597392362493096 1102 36-37 0.3878041898846334 1102 38-39 0.18167669566081202 1102 40-41 0.11479540219232831 1102 42-43 0.251000222745013 1102 44-45 0.3295285224057025 1102 46-47 0.4628272356396437 1102 48-49 0.43298530614934094 1102 50-51 0.2361038804434159 1102 52-53 0.2702734164131684 1102 54-55 0.4199298641361935 1102 56-57 0.35351104828555435 1102 58-59 0.48729494041222665 1102 60-61 0.35500855032808687 1102 62-63 0.28792816441941227 1102 64-65 0.23551759903321567 1102 66-67 0.556008255590811 1102 68-69 0.5176925868831646 1102 70-71 0.5987504856678179 1102 72-73 0.7173646082415495 1102 74-75 0.8377394497135455 1102 76-77 0.6862338557632341 1102 78-79 0.7700396545153261 1102 80-81 0.9992490386156128 1102 82-83 0.939049010853811 1102 84-85 1.0954716067880241 1102 86-87 0.9927125287652387 1102 88-89 0.927849872819575 1102 90-91 0.8696882781234905 1102 92-93 1.2012270487512744 1102 94-95 1.1622466816973294 1102 96-97 0.9397443025156882 1102 98-99 0.7835647809753219 1102 100-101 1.0208595254784179 1103 1 0.08075437314175815 1103 2 0.1003777805092696 1103 3 0.17915861196559746 1103 4 0.02846153894887493 1103 5 0.13599225605604204 1103 6 0.09077442024982219 1103 7 0.18523903171340805 1103 8 0.09143490502145823 1103 9 0.16352972272228783 1103 10-11 0.10216289905198295 1103 12-13 0.12645010417146096 1103 14-15 0.09204453724983352 1103 16-17 0.14077200522444855 1103 18-19 0.06124716656554341 1103 20-21 0.042272910678221365 1103 22-23 -0.015977819941198845 1103 24-25 0.04212997847701416 1103 26-27 0.040955740852211875 1103 28-29 -0.14418926550240485 1103 30-31 0.050516308442439595 1103 32-33 0.0837676164094816 1103 34-35 0.09757906207018863 1103 36-37 -0.002440430167780505 1103 38-39 0.05881554236998454 1103 40-41 0.19626317991588138 1103 42-43 0.06490082467912117 1103 44-45 0.41830268086047795 1103 46-47 0.20940852544924127 1103 48-49 0.3384881809085911 1103 50-51 0.42258289599266874 1103 52-53 0.483088484572086 1103 54-55 0.682816765395458 1103 56-57 0.48040554763539944 1103 58-59 0.4941183673319145 1103 60-61 0.28487583490773005 1103 62-63 0.5046868769635822 1103 64-65 0.43641239116931985 1103 66-67 0.49675384155084856 1103 68-69 0.5519654435872496 1103 70-71 0.7411551220244057 1103 72-73 0.7287796844377432 1103 74-75 0.5999661466539301 1103 76-77 0.44533211712951015 1103 78-79 0.3823687548864747 1103 80-81 0.44781876336782034 1103 82-83 0.43816634638399776 1103 84-85 0.6739056739049687 1103 86-87 0.8511177191193653 1103 88-89 0.8654709700791479 1103 90-91 0.7507560581434127 1103 92-93 0.7753920338105651 1103 94-95 0.7322333627928117 1103 96-97 0.83348154693768 1103 98-99 0.8051692496974248 1103 100-101 0.880167832283977 1104 1 0.029705937029739005 1104 2 -7.02213880366287E-4 1104 3 0.08181885788639676 1104 4 0.13814993017800958 1104 5 0.045802789507817465 1104 6 0.010987497065009677 1104 7 0.08084541817373747 1104 8 -0.02699337587058892 1104 9 0.04346508669442528 1104 10-11 0.0017184533088396847 1104 12-13 0.11166884978836578 1104 14-15 0.239003838759956 1104 16-17 0.09823261581868081 1104 18-19 0.2021577981986269 1104 20-21 0.17736571539091983 1104 22-23 0.05052394498470392 1104 24-25 0.09288620831478056 1104 26-27 0.05234740245397518 1104 28-29 0.09087899388326548 1104 30-31 0.1848435327165987 1104 32-33 0.0923453315901881 1104 34-35 0.15311724758610268 1104 36-37 0.1981284954481879 1104 38-39 0.14904883985934703 1104 40-41 0.2632938381981802 1104 42-43 0.30709015096833525 1104 44-45 0.40972642671115267 1104 46-47 0.18124276267398187 1104 48-49 0.31703731831566273 1104 50-51 0.247668220224746 1104 52-53 0.3608659052088683 1104 54-55 0.6190471291338326 1104 56-57 0.4300686338011843 1104 58-59 0.5760559286952329 1104 60-61 0.579368623256876 1104 62-63 0.5551202188682964 1104 64-65 0.6294248625331065 1104 66-67 0.7166463680831825 1104 68-69 0.4904224191354274 1104 70-71 0.3910481745280272 1104 72-73 0.5489997742035122 1104 74-75 0.5638888990032669 1104 76-77 0.6505734210707885 1104 78-79 0.7251486300559549 1104 80-81 0.539372833216806 1104 82-83 1.0236469088226379 1104 84-85 0.8449135517861208 1104 86-87 0.7939095747460918 1104 88-89 0.7973971353977696 1104 90-91 0.9208990054527781 1104 92-93 1.2101336065816177 1104 94-95 0.9810426222252246 1104 96-97 1.0345852100355337 1104 98-99 1.070844655279732 1104 100-101 1.236804128978541 1105 1 0.049964868486860325 1105 2 0.04314086097729586 1105 3 -0.031027026309210726 1105 4 0.05299032000472437 1105 5 0.09019126127769539 1105 6 0.07276651653148036 1105 7 0.1813320800338687 1105 8 0.18111361515445168 1105 9 0.18156859712377837 1105 10-11 0.18897372392515166 1105 12-13 0.2701625909314984 1105 14-15 0.36799564692940834 1105 16-17 0.2591884122535575 1105 18-19 0.30849155003215145 1105 20-21 0.2766621378357925 1105 22-23 0.33365564202370734 1105 24-25 0.32407856094884835 1105 26-27 0.4396359251897195 1105 28-29 0.2637465074948864 1105 30-31 0.29634068608655895 1105 32-33 0.4801637128645737 1105 34-35 0.6513000347974014 1105 36-37 0.5605167745422364 1105 38-39 0.4960828751128048 1105 40-41 0.5949534297254147 1105 42-43 0.6786484012231639 1105 44-45 0.5788756075280972 1105 46-47 0.9088566278754868 1105 48-49 0.8959240870797984 1105 50-51 0.930517585016041 1105 52-53 0.7111016223358817 1105 54-55 1.0701113523394667 1105 56-57 1.1710711385870098 1105 58-59 1.1693032623103292 1105 60-61 1.1790977894055956 1105 62-63 1.1508824382452687 1105 64-65 1.2941028763560425 1105 66-67 1.272255876324735 1105 68-69 1.1967572586551114 1105 70-71 1.1370843354309947 1105 72-73 1.200920006408964 1105 74-75 1.2100669252569034 1105 76-77 1.1078840704504955 1105 78-79 1.2602459313512355 1105 80-81 1.2351283710931469 1105 82-83 1.4045662522331206 1105 84-85 1.2280354596497816 1105 86-87 1.1922388888201283 1105 88-89 1.308366450217651 1105 90-91 1.304496387547598 1105 92-93 1.5475043273135363 1105 94-95 1.569720897092843 1105 96-97 1.6382082926002361 1105 98-99 1.9309907470680123 1105 100-101 1.5298011393954702 1106 1 0.06956505051898887 1106 2 -0.4861619596140869 1106 3 -0.1718589711339007 1106 4 0.08536538595510734 1106 5 0.060227577049040804 1106 6 -0.1534020517423471 1106 7 0.01607628319912635 1106 8 0.029386032392380912 1106 9 0.09952631998168471 1106 10-11 -0.30894305983554915 1106 12-13 -0.2422388007802425 1106 14-15 -0.16010646874075718 1106 16-17 -0.10630770687232882 1106 18-19 -0.07629063692788662 1106 20-21 -0.00297791358284627 1106 22-23 -0.07510256891236367 1106 24-25 -0.034308514770351906 1106 26-27 -0.13381104526268928 1106 28-29 0.05091895392322954 1106 30-31 0.048869917290929266 1106 32-33 0.1057980603682509 1106 34-35 0.22957974057256791 1106 36-37 0.16532439885984473 1106 38-39 0.1782251430082482 1106 40-41 0.33996930314419416 1106 42-43 0.016946219557276265 1106 44-45 -0.03310043820475528 1106 46-47 0.14310196511538464 1106 48-49 0.29482237572400294 1106 50-51 -0.03579831324179139 1106 52-53 0.22627746282932293 1106 54-55 0.25597313121394905 1106 56-57 0.18402914247059954 1106 58-59 -0.13631988042715193 1106 60-61 -0.012462918489042352 1106 62-63 0.06648770412277116 1106 64-65 0.03855896473228171 1106 66-67 0.29113309710758273 1106 68-69 0.36743367842408503 1106 70-71 0.3582479268305221 1106 72-73 0.41931866763884074 1106 74-75 0.27001197113661846 1106 76-77 0.28035588176078363 1106 78-79 0.3660697808229969 1106 80-81 0.43443496286797867 1106 82-83 0.5156792771392524 1106 84-85 0.23873120673391313 1106 86-87 0.241085686305766 1106 88-89 0.41860512261603233 1106 90-91 0.35223222702572343 1106 92-93 0.8242344030454269 1106 94-95 0.5298546834516742 1106 96-97 0.4240241101764859 1106 98-99 0.29201175872423235 1106 100-101 -0.0367267066242114 1107 1 0.03595432162524048 1107 2 0.11667953851043222 1107 3 0.05425359554470788 1107 4 0.07492299815422854 1107 5 -0.016419360918654036 1107 6 -0.001066651293740506 1107 7 0.08138173246683778 1107 8 -0.05109198016109673 1107 9 0.09032797116068991 1107 10-11 0.07580898057687335 1107 12-13 0.024651855733054617 1107 14-15 0.13228698686024387 1107 16-17 0.08774540982229695 1107 18-19 0.08515405046019708 1107 20-21 0.09941921758787231 1107 22-23 0.04711059528193573 1107 24-25 0.061876903584881404 1107 26-27 0.09808111917664064 1107 28-29 0.08993559765684722 1107 30-31 0.11140078265198383 1107 32-33 0.12718799119214452 1107 34-35 0.09741002349719707 1107 36-37 0.10832008242157087 1107 38-39 0.14135305283427613 1107 40-41 0.014820395448118973 1107 42-43 0.06920245388898394 1107 44-45 0.07634247737023969 1107 46-47 0.37296219922090046 1107 48-49 0.4365740203058408 1107 50-51 0.47691350324361537 1107 52-53 0.4848175076901313 1107 54-55 0.6912864028479717 1107 56-57 0.744350424961695 1107 58-59 0.7834447888658147 1107 60-61 0.5995854105404206 1107 62-63 0.5121133049370528 1107 64-65 0.42535565860443825 1107 66-67 0.4395511884502028 1107 68-69 0.48950971559446543 1107 70-71 0.5959686966267661 1107 72-73 0.6070606426449672 1107 74-75 0.5873526493263483 1107 76-77 0.5450600455215522 1107 78-79 0.7406452053984189 1107 80-81 0.7781813108596118 1107 82-83 0.6080307935473712 1107 84-85 0.5575298609099271 1107 86-87 0.687499649700861 1107 88-89 0.9439740932680216 1107 90-91 0.9963739343749758 1107 92-93 1.1379282563619206 1107 94-95 1.2936088538096158 1107 96-97 1.2046081487793963 1107 98-99 1.3086111970217864 1107 100-101 1.2428408955849015 1108 1 -0.021116759914633576 1108 2 0.05627388788897747 1108 3 0.14011240805567837 1108 4 0.043918725066873776 1108 5 0.10782114170568491 1108 6 0.08710821358694432 1108 7 0.015382044655865457 1108 8 0.02383728951168962 1108 9 0.030055976467906476 1108 10-11 0.12855429286345554 1108 12-13 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0.8030716265883058 2316 56-57 0.6510066094480678 2316 58-59 0.6876033228063889 2316 60-61 0.8437479204804177 2316 62-63 0.9338023817822716 2316 64-65 1.0489929751662856 2316 66-67 1.123385136256168 2316 68-69 1.247624368959606 2316 70-71 1.3258320668284576 2316 72-73 1.2034406758987792 2316 74-75 0.9242734185015138 2316 76-77 0.7955227072724327 2316 78-79 0.6871969295363485 2316 80-81 1.124470980490205 2316 82-83 1.5616543748384863 2316 84-85 1.7285900069375408 2316 86-87 1.4462215466140407 2316 88-89 1.7114215016393075 2316 90-91 1.9828085472572177 2316 92-93 1.6541286467327723 2316 94-95 2.0928606430093595 2316 96-97 2.7879360602953334 2316 98-99 3.4755561836479707 2316 100-101 4.032120155326098 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3086.0 3 406.0 4 369.0 5 364.0 6 390.0 7 415.0 8 551.0 9 702.0 10 887.0 11 1277.0 12 2171.0 13 3394.0 14 3956.0 15 5723.0 16 8824.0 17 13779.0 18 11024.0 19 5781.0 20 5170.0 21 4923.0 22 5138.0 23 5346.0 24 5998.0 25 6706.0 26 7833.0 27 9249.0 28 10962.0 29 13356.0 30 16833.0 31 21574.0 32 28502.0 33 38776.0 34 56298.0 35 84896.0 36 117158.0 37 148083.0 38 102275.0 39 10690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.75259786701445 19.164922484696774 15.301252655714256 20.781226992574517 2 14.893457639325131 20.88188214761643 41.20430848002019 23.020351733038254 3 18.76384796738779 27.095757626731075 30.43766804828752 23.702726357593615 4 11.498233113386942 16.600717793579808 38.24675435610814 33.6542947369251 5 12.143326499500604 38.99897492509068 33.258818272617354 15.598880302791358 6 29.192591463735063 37.26457481581132 17.832669448327326 15.71016427212629 7 26.74822510549521 35.59156143420935 20.153263651316653 17.506949808978785 8 27.207182589000677 32.79276486059692 19.622320257917377 20.377732292485028 9 27.936738182424964 15.906376504255077 20.62683700109448 35.530048312225475 10-11 24.474971765257767 27.529120054978083 26.218200190399727 21.77770798936442 12-13 26.392642647574725 25.40070872131906 27.73348452020723 20.47316411089899 14-15 23.012093857954483 25.56572336194931 28.395309072922466 23.026873707173745 16-17 20.085192751062447 29.561490511879036 28.31098966783366 22.042327069224857 18-19 19.90014298127451 26.456395965524237 33.45177288242237 20.191688170778885 20-21 21.420159539705946 25.04576339650773 33.5017618989757 20.032315164810623 22-23 20.63865184154751 25.387523964714482 33.67913455772582 20.29468963601219 24-25 20.353013349167025 25.522170231436352 33.88674620293571 20.23807021646091 26-27 19.812539530435892 25.857157775616212 33.95364783414261 20.37665485980529 28-29 19.600096957339428 26.32372058681525 33.87010212708375 20.20608032876158 30-31 19.7409204563521 25.889493731925334 33.86593244590903 20.503653365813534 32-33 19.64924566204766 26.07961047441242 33.6622079510623 20.608935912477616 34-35 20.189990205539157 26.25703792118317 33.33959583232882 20.21337604094886 36-37 20.171759799011475 25.899801310366342 33.48626414226835 20.442174748353832 38-39 19.936124435851227 26.26720029842748 33.34184919955236 20.454826066168938 40-41 19.87426348321543 26.416489569897838 33.42945594296477 20.27979100392196 42-43 19.772463498723468 26.48932893087007 32.760540197217566 20.977667373188893 44-45 19.79350703087543 26.705964257800957 32.60933815850831 20.891190552815306 46-47 20.306255698284538 27.15483831979758 31.73448555821512 20.804420423702762 48-49 20.39062125359573 27.37035511560521 31.170806145728847 21.06821748507022 50-51 20.30645576672413 27.731139364251778 31.008624858091917 20.95378001093218 52-53 20.62348055770924 27.86489611942652 30.463947330380964 21.047675992483278 54-55 20.06712295561726 27.681026039213524 30.75864442898519 21.49320657618403 56-57 20.571053663361262 27.988078749083016 30.161841883179356 21.27902570437636 58-59 20.86450649489661 27.459940859371258 30.435687685471148 21.23986496026099 60-61 20.465297405776848 27.637959295178256 31.066518340285587 20.83022495875931 62-63 20.341382908788333 27.776190673210355 30.99785970739921 20.884566710602098 64-65 20.175808415320788 27.90784570992531 30.88999562646031 21.026350248293596 66-67 20.252465452147867 27.89814170874534 30.49234178058937 21.357051058517428 68-69 20.328584572404647 27.775949899948316 30.69127274804904 21.204192779597992 70-71 20.38053632610119 27.957966576322345 30.46562898758975 21.195868109986716 72-73 20.448186998084942 27.890452810461635 30.43939979036796 21.221960401085465 74-75 20.164215539791925 28.580820197644474 29.99363602391384 21.26132823864976 76-77 20.106472069965484 28.145080510882025 30.18795177557448 21.56049564357801 78-79 20.17270755580778 28.259170367918195 30.20359182322271 21.364530253051317 80-81 20.30616715060815 28.50994406157496 29.85517221902222 21.328716568794672 82-83 20.203941976294555 28.285242938802956 29.942837928715534 21.567977156186956 84-85 20.198352165384968 28.197579927596138 29.750526480992512 21.853541426026382 86-87 20.133595305514547 28.310463302005246 29.803231924182167 21.75270946829804 88-89 20.165867408525333 28.209346526018248 29.523952131201803 22.100833934254617 90-91 20.070060406413404 28.219935035851123 29.639269028545613 22.070735529189868 92-93 20.30318533750418 28.140530252529665 29.77414780843447 21.782136601531686 94-95 20.135732045450666 27.88023426715334 29.739161729184755 22.244871958211238 96-97 20.12667921549259 27.808675503386997 29.797988422328853 22.266656858791556 98-99 20.28371160671828 27.924404872935348 29.23564168109439 22.556241839251985 100-101 20.584743169284074 27.137016221924227 29.926829941014766 22.351410667776936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1602.0 1 1011.0 2 647.5 3 858.0 4 659.0 5 595.5 6 1260.5 7 3058.0 8 5001.0 9 5257.0 10 3968.0 11 2905.0 12 2659.0 13 2673.0 14 2764.0 15 2721.0 16 2637.0 17 2682.0 18 2794.5 19 2865.5 20 3003.0 21 3323.0 22 3727.5 23 4301.0 24 4982.0 25 5715.0 26 6505.5 27 7538.0 28 8536.5 29 9637.5 30 10854.0 31 12139.5 32 14014.0 33 15541.5 34 16970.5 35 17903.0 36 18888.5 37 20651.0 38 21547.0 39 21640.5 40 21613.0 41 22227.0 42 22757.0 43 23674.0 44 25005.5 45 25600.5 46 25416.0 47 26642.0 48 30297.0 49 29637.0 50 25219.5 51 22172.0 52 20241.0 53 19916.5 54 19804.0 55 18339.0 56 16462.0 57 14931.0 58 13713.0 59 12365.5 60 10769.0 61 9225.5 62 7814.5 63 5986.5 64 4377.5 65 3513.5 66 2564.0 67 1872.5 68 1378.5 69 905.5 70 696.0 71 547.5 72 435.5 73 381.5 74 297.5 75 235.5 76 179.0 77 121.5 78 92.0 79 67.5 80 41.0 81 25.5 82 18.0 83 11.5 84 8.0 85 7.0 86 4.5 87 2.5 88 3.5 89 4.0 90 2.5 91 2.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29376101931534415 2 0.2552220904091812 3 0.2520760553964332 4 0.2519449706042354 5 0.2549599208247855 6 0.22140221402214022 7 0.22192655319093155 8 0.22166438360653587 9 0.23254442135895603 10-11 0.24047505128692495 12-13 0.2328065909433517 14-15 0.22199209558703045 16-17 0.21628990712642474 18-19 0.20665517489988397 20-21 0.211374227419006 22-23 0.20835927719845582 24-25 0.2122262785682919 26-27 0.20770385323746668 28-29 0.2235651130934045 30-31 0.23411743886533 32-33 0.22730102967104274 34-35 0.22553138497637196 36-37 0.2133404993019735 38-39 0.2020016647768609 40-41 0.1980691210109259 42-43 0.21294724492537997 44-45 0.2071139716725764 46-47 0.21904268776257924 48-49 0.2252692153919763 50-51 0.2371323890858802 52-53 0.24840568121489384 54-55 0.22605572414516328 56-57 0.20128069841977284 58-59 0.19131825421273752 60-61 0.19413657724499092 62-63 0.1993144265368053 64-65 0.19243247494641907 66-67 0.19577513714746386 68-69 0.19774140903043133 70-71 0.19905225695240966 72-73 0.2006908168548826 74-75 0.20298480071834465 76-77 0.21399592326296263 78-79 0.21445472003565505 80-81 0.20731059886087316 82-83 0.19937996893290422 84-85 0.18895872795317653 86-87 0.19151488140103426 88-89 0.2010840712314761 90-91 0.19951105372510206 92-93 0.20298480071834465 94-95 0.19820020580312375 96-97 0.20606529333499374 98-99 0.20960458272433524 100-101 0.19505417079037576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 762865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.00688452103353 #Duplication Level Percentage of deduplicated Percentage of total 1 77.87896549691638 42.060002962095176 2 12.368050173096652 13.359197148975593 3 3.9836884314264074 6.454398032514696 4 1.7755773961036374 3.835736135581062 5 0.9973153043154966 2.6930946235613216 6 0.6111397750320049 1.9803453153818342 7 0.4280226511596677 1.6181318925496755 8 0.2934759191819817 1.2679776061572363 9 0.2348643181165735 1.1415861095969762 >10 1.300774071068269 13.01447180805622 >50 0.08071745312869862 3.000952167681666 >100 0.043275917235641205 4.3393090904171965 >500 0.001944985044092568 0.8282607643643408 >1k 0.001458738783069426 1.1402372348985315 >5k 4.86246261023142E-4 1.8071263316338657 >10k+ 2.43123130511571E-4 1.4591727765347051 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11113 1.45674529569452 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7657 1.0037162538588087 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6106 0.8004037411599694 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1754 0.22992272551499937 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1699 0.22271306194411852 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1612 0.2113086850229071 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1277 0.16739527963663295 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1183 0.15507330917003664 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1159 0.15192727415728863 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 950 0.1245305525879415 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 934 0.12243319591277617 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 843 0.11050447982277337 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 830 0.10880037752420152 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.10735844481002535 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3108479219783316E-4 0.0 10-11 0.0 0.0 0.0 1.3108479219783316E-4 0.0 12-13 0.0 0.0 0.0 1.3108479219783316E-4 0.0 14-15 0.0 0.0 0.0 1.3108479219783316E-4 0.0 16-17 0.0 0.0 0.0 1.9662718829674975E-4 0.0 18-19 0.0 0.0 0.0 2.6216958439566633E-4 0.0 20-21 0.0 0.0 0.0 2.6216958439566633E-4 0.0 22-23 0.0 0.0 0.0 3.932543765934995E-4 0.0 24-25 0.0 0.0 0.0 9.175935453848322E-4 0.0 26-27 0.0 0.0 0.0 0.0013108479219783318 0.0 28-29 0.0 0.0 0.0 0.0016385599024729145 0.0 30-31 0.0 0.0 0.0 0.0035392893893414954 0.0 32-33 0.0 0.0 0.0 0.00786508753186999 0.0 34-35 0.0 0.0 0.0 0.012911852031486568 0.0 36-37 0.0 0.0 0.0 0.020645854771158725 0.0 38-39 0.0 0.0 0.0 0.038080132133470536 0.0 40-41 0.0 0.0 0.0 0.06134768274858592 0.0 42-43 0.0 1.3108479219783316E-4 0.0 0.08317330064952513 0.0 44-45 0.0 1.3108479219783316E-4 0.0 0.10998014065398203 0.0 46-47 0.0 1.3108479219783316E-4 0.0 0.14707713684596882 0.0 48-49 0.0 1.3108479219783316E-4 0.0 0.19118716942053968 0.0 50-51 0.0 1.3108479219783316E-4 0.0 0.24709483329291554 0.0 52-53 0.0 1.3108479219783316E-4 0.0 0.3043133450872697 0.0 54-55 0.0 1.3108479219783316E-4 0.0 0.3695935716017906 0.0 56-57 0.0 1.3108479219783316E-4 0.0 0.4587312302963171 0.0 58-59 0.0 1.3108479219783316E-4 0.0 0.5462303290883708 0.0 60-61 0.0 1.3108479219783316E-4 0.0 0.6570625208916387 0.0 62-63 0.0 1.3108479219783316E-4 0.0 0.7809376495185911 0.0 64-65 0.0 1.3108479219783316E-4 0.0 0.9050094053338402 0.0 66-67 0.0 1.3108479219783316E-4 0.0 1.051168948634424 0.0 68-69 0.0 1.3108479219783316E-4 0.0 1.2350153696918853 0.0 70-71 0.0 1.3108479219783316E-4 0.0 1.4569419228828169 0.0 72-73 0.0 1.3108479219783316E-4 0.0 1.6891586322612782 0.0 74-75 0.0 1.3108479219783316E-4 0.0 1.9192124425684756 0.0 76-77 0.0 1.3108479219783316E-4 0.0 2.193769539826837 0.0 78-79 0.0 1.3108479219783316E-4 0.0 2.5044404973357013 0.0 80-81 0.0 1.3108479219783316E-4 0.0 2.853715926146828 0.0 82-83 0.0 1.3108479219783316E-4 0.0 3.2236372097291133 0.0 84-85 0.0 1.3108479219783316E-4 0.0 3.567407077267931 0.0 86-87 0.0 1.3108479219783316E-4 0.0 3.9353620889672483 0.0 88-89 0.0 1.3108479219783316E-4 0.0 4.373054210115813 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4755 0.0 39.591545 1 GTATCAA 7850 0.0 37.789635 1 CCGACTA 75 3.0754032E-4 31.670305 9 TATCAAC 10060 0.0 29.56494 2 ATCAACG 10080 0.0 29.506277 3 TCAACGC 10170 0.0 29.247084 4 CAACGCA 10255 0.0 29.008482 5 AACGCAG 10430 0.0 28.51051 6 GTACATG 6280 0.0 27.403542 1 TACATGG 6240 0.0 27.258318 2 GTGTACG 70 0.008355568 27.165627 1 ACATGGG 6340 0.0 25.329584 3 ACGCAGA 11730 0.0 25.271444 7 CGCAGAG 11785 0.0 25.113195 8 GAGTACT 6630 0.0 24.53445 12-13 TAAGGTG 310 0.0 24.527012 5 GCAGAGT 12000 0.0 24.30696 9 AGTACTT 6715 0.0 24.294615 12-13 GTGTAAG 285 3.6379788E-12 23.352907 1 CATGGGA 4385 0.0 23.08018 4 >>END_MODULE