FastQCFastQC Report
Thu 26 May 2016
SRR937159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762865
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104521.3700982480517523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86571.1348010460566418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69490.9109082209827427No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30210.396007157229654No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.2548288360325877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18560.24329337431917836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12400.16254514232531314No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.11968041527662168No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.10526108813486003No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7930.10395024021288171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77400.028.2982961
GTATCAA107400.026.8524991
AGTACTT80200.024.31384512-13
GTGTACG1050.002192416722.6246051
GAGTACT79500.022.40675712-13
TAAGGTG3251.8189894E-1221.9212765
TACTTTT88750.021.86158414-15
GTACTTT83550.021.28939214-15
TATCAAC136050.021.2257482
GTACATG65900.021.124311
AGAGTAC123600.021.11855110-11
TACATGG65900.021.0453132
ATCAACG138400.020.7967033
TAGACAG2751.2587407E-920.725575
TCAACGC139400.020.6475144
CAACGCA140750.020.4494745
GTACACG1404.8416512E-420.3621441
AACGCAG144150.020.1318876
ATACAGT3353.45608E-1119.8491156
ATTCGCG1201.4151738E-719.7900480-81
Produced by FastQC (version 0.11.5)