##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937159_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 762865 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.325268559968016 31.0 31.0 33.0 30.0 34.0 2 31.5448722906412 31.0 31.0 34.0 30.0 34.0 3 31.524261828763937 31.0 31.0 34.0 30.0 34.0 4 35.38689545332398 37.0 35.0 37.0 33.0 37.0 5 35.28687120263743 37.0 35.0 37.0 33.0 37.0 6 35.397635230348754 37.0 35.0 37.0 33.0 37.0 7 35.300366381994195 37.0 35.0 37.0 32.0 37.0 8 35.27679733635702 37.0 35.0 37.0 32.0 37.0 9 36.76794714661179 39.0 37.0 39.0 32.0 39.0 10-11 36.638576943495906 38.0 35.0 39.0 32.0 39.0 12-13 36.47390495041718 38.0 35.0 39.0 32.0 39.0 14-15 37.24818480334004 39.0 36.0 40.0 32.0 41.0 16-17 37.215429990889604 39.0 36.0 40.0 32.0 41.0 18-19 37.308697475962326 39.0 36.0 40.0 32.0 41.0 20-21 37.22377681503281 39.0 36.0 40.0 31.5 41.0 22-23 37.12387381777903 39.0 36.0 40.0 31.0 41.0 24-25 37.091185858572615 39.0 36.0 40.0 31.0 41.0 26-27 36.78764198121554 38.5 35.5 40.0 30.5 41.0 28-29 36.5333551807987 38.0 35.0 40.0 30.0 41.0 30-31 36.38632523447792 38.0 35.0 40.0 30.0 41.0 32-33 36.078873719465435 38.0 35.0 40.0 30.0 41.0 34-35 35.763728837998855 38.0 35.0 40.0 30.0 41.0 36-37 35.43176053430162 38.0 34.0 40.0 28.5 41.0 38-39 35.18391524057336 38.0 34.0 40.0 27.5 41.0 40-41 34.79177377386563 38.0 33.5 40.0 26.5 41.0 42-43 34.363542042169975 37.0 33.0 40.0 24.5 41.0 44-45 33.69791378553217 37.0 33.0 40.0 23.5 41.0 46-47 32.92200651491417 37.0 32.0 39.5 19.0 40.5 48-49 32.2450656407097 36.5 31.0 39.0 11.5 40.0 50-51 31.784343232419893 36.0 30.5 39.0 8.0 40.0 52-53 31.38594508858055 36.0 30.0 39.0 6.5 40.0 54-55 31.026087839919253 35.0 29.5 39.0 2.0 40.0 56-57 30.543723332437587 35.0 28.5 38.5 2.0 40.0 58-59 30.122751731957816 35.0 28.0 38.0 2.0 40.0 60-61 29.58109691754111 34.0 27.5 38.0 2.0 40.0 62-63 29.033863789792427 34.0 26.0 38.0 2.0 40.0 64-65 28.439621689289716 33.5 26.0 37.0 2.0 39.0 66-67 27.790837173025373 33.0 24.5 36.5 2.0 39.0 68-69 27.155815249093877 32.0 23.0 36.0 2.0 39.0 70-71 26.571953097861353 32.0 22.0 35.5 2.0 38.0 72-73 25.931270932602754 31.0 20.5 35.0 2.0 38.0 74-75 25.292336783048114 31.0 19.0 35.0 2.0 37.0 76-77 24.41923472698315 29.5 18.5 34.0 2.0 36.0 78-79 24.96097933448251 31.0 18.5 34.0 2.0 36.0 80-81 24.646080237001303 31.0 17.5 34.0 2.0 36.0 82-83 24.03366650718017 30.5 10.5 34.0 2.0 35.5 84-85 23.336140077208942 30.0 7.0 34.0 2.0 35.0 86-87 22.678427375748 29.0 2.0 34.0 2.0 35.0 88-89 21.870060233462016 29.0 2.0 34.0 2.0 35.0 90-91 20.901310847921977 28.0 2.0 33.0 2.0 35.0 92-93 19.948717007596365 26.5 2.0 33.0 2.0 34.5 94-95 19.003840784411395 25.5 2.0 32.5 2.0 34.0 96-97 17.85498941490303 24.0 2.0 32.0 2.0 34.0 98-99 16.44895296022232 10.5 2.0 32.0 2.0 34.0 100-101 14.063719662063406 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2018381621062666 1101 2 0.31621974323497426 1101 3 0.2198001589063523 1101 4 0.18434893116111795 1101 5 0.12212314434471949 1101 6 0.21801119288674187 1101 7 0.2725554909441712 1101 8 0.14196773632088622 1101 9 0.25759687276266163 1101 10-11 0.0869048203959153 1101 12-13 0.1457851204181182 1101 14-15 0.3318926572062324 1101 16-17 0.4946484084208649 1101 18-19 0.3716480550480057 1101 20-21 0.37908457713657384 1101 22-23 0.14405003098195834 1101 24-25 0.39775248932554774 1101 26-27 0.1963866095168072 1101 28-29 -0.056338426147917176 1101 30-31 0.3048829979769323 1101 32-33 0.21140540187518297 1101 34-35 0.4610205631234763 1101 36-37 0.49378315722255195 1101 38-39 0.5573091368140055 1101 40-41 0.748232435440201 1101 42-43 0.5451227553702154 1101 44-45 0.3812732675307089 1101 46-47 0.5764452691163271 1101 48-49 0.31251757493591015 1101 50-51 0.4217708618895948 1101 52-53 0.5002292387270124 1101 54-55 0.5006486095463849 1101 56-57 0.6337732986340079 1101 58-59 0.5092327131327998 1101 60-61 0.5481368917415601 1101 62-63 0.7540402356377776 1101 64-65 0.8566858119665746 1101 66-67 0.6570985089155847 1101 68-69 0.705263031623172 1101 70-71 0.8588462008112643 1101 72-73 1.006547983356736 1101 74-75 1.0462034949408405 1101 76-77 0.9920286717115978 1101 78-79 1.1006753154903457 1101 80-81 1.2632752381029064 1101 82-83 1.124440026623752 1101 84-85 1.4082513372227652 1101 86-87 1.661948555391529 1101 88-89 1.9811697984153476 1101 90-91 2.4111885949832903 1101 92-93 2.489154295225344 1101 94-95 2.6771893772572106 1101 96-97 2.9028613323829084 1101 98-99 3.070704918371863 1101 100-101 3.1170246118420373 1102 1 0.23176491595108573 1102 2 0.24764343958291235 1102 3 0.22427575941240008 1102 4 0.11323099873201414 1102 5 0.2250470135974183 1102 6 0.11787443447382628 1102 7 0.11540712443162704 1102 8 0.2556558161388409 1102 9 0.2466533535602764 1102 10-11 0.17865463076991261 1102 12-13 0.22932159905263916 1102 14-15 0.3762512659419812 1102 16-17 0.23403362171987396 1102 18-19 0.2521395599455545 1102 20-21 0.31512511511941455 1102 22-23 0.30202058451909863 1102 24-25 0.12474477193872957 1102 26-27 0.21342911277923093 1102 28-29 0.22188525141530846 1102 30-31 0.35172204285958486 1102 32-33 0.4217273178022012 1102 34-35 0.512345881645345 1102 36-37 0.7053164737634887 1102 38-39 0.5207184343937641 1102 40-41 0.48717151177302753 1102 42-43 0.5792536563676549 1102 44-45 0.670654994272887 1102 46-47 0.6995150329839319 1102 48-49 0.953360951687543 1102 50-51 0.9377012246765055 1102 52-53 0.9589181652802061 1102 54-55 0.9608520536953016 1102 56-57 1.0504008304790808 1102 58-59 0.9381051748838516 1102 60-61 1.0479131117162552 1102 62-63 1.023817907158584 1102 64-65 1.2475338137425815 1102 66-67 1.2812604900767965 1102 68-69 1.2074841106342404 1102 70-71 0.9513395727955718 1102 72-73 1.3400424810008182 1102 74-75 1.34625586444756 1102 76-77 1.2205447219692438 1102 78-79 1.29666356935153 1102 80-81 1.5675025815316026 1102 82-83 1.4792356076620088 1102 84-85 1.669019105499082 1102 86-87 1.832510204120215 1102 88-89 2.290946459546216 1102 90-91 2.2247599107244547 1102 92-93 2.3293049144805273 1102 94-95 2.407609870242119 1102 96-97 2.694978583154576 1102 98-99 2.9205766273112523 1102 100-101 2.8814002548983098 1103 1 0.1880852241229789 1103 2 0.20436848170681898 1103 3 0.02584417523778626 1103 4 0.10592282396731889 1103 5 -0.03679504256587762 1103 6 -0.0082654943963405 1103 7 0.10915886464409397 1103 8 -0.15407703074829016 1103 9 -0.10160370703787436 1103 10-11 0.07497097771589267 1103 12-13 0.1239971229821819 1103 14-15 -0.07393667700488749 1103 16-17 0.16568812244753417 1103 18-19 0.17631114704803252 1103 20-21 0.22062260777822473 1103 22-23 0.11665243449771623 1103 24-25 0.02227245028718272 1103 26-27 -0.06971960254927723 1103 28-29 -0.05671379502002338 1103 30-31 0.07933250508872902 1103 32-33 -0.21560917398686996 1103 34-35 -0.11586364424184126 1103 36-37 -0.07333424083592632 1103 38-39 0.28488878720121846 1103 40-41 0.312593037878365 1103 42-43 0.35231236862478 1103 44-45 -0.0035693374137650835 1103 46-47 0.228651571848431 1103 48-49 0.4963147775715697 1103 50-51 0.5214890464902346 1103 52-53 0.39944774960982343 1103 54-55 0.26992957603460965 1103 56-57 0.3009842501652251 1103 58-59 0.10341925991242462 1103 60-61 0.3865889571365919 1103 62-63 0.38036196718551096 1103 64-65 0.7326386714111415 1103 66-67 0.6636458082809256 1103 68-69 0.6855886496824546 1103 70-71 0.738448078810606 1103 72-73 0.5281533493991368 1103 74-75 0.4380705508361551 1103 76-77 0.736963357527852 1103 78-79 0.504466107980658 1103 80-81 0.9079286860103686 1103 82-83 0.8293986494242347 1103 84-85 0.7991547057252717 1103 86-87 1.0644378285041434 1103 88-89 1.209565154236163 1103 90-91 1.50902343845447 1103 92-93 1.283475252935709 1103 94-95 1.2486545574631442 1103 96-97 1.4687104167716036 1103 98-99 2.0500473587315255 1103 100-101 2.0973480643116744 1104 1 0.1528948225146749 1104 2 0.19385197789645048 1104 3 0.23037980102674283 1104 4 0.18958732441146964 1104 5 0.11805382834907618 1104 6 0.14106359999797746 1104 7 0.21664694262810968 1104 8 -0.04925679604821198 1104 9 0.11891852401546998 1104 10-11 0.050790908521449296 1104 12-13 0.194886365115984 1104 14-15 0.07205980483157504 1104 16-17 0.1956261162761308 1104 18-19 0.13636654935763204 1104 20-21 0.3821630023735736 1104 22-23 0.3348428594825208 1104 24-25 0.20230073585452857 1104 26-27 0.2866887732513632 1104 28-29 0.17686713442969904 1104 30-31 0.28143843570726546 1104 32-33 0.3285753367429507 1104 34-35 0.2863188444205633 1104 36-37 0.4869113877582194 1104 38-39 0.5187926045839788 1104 40-41 0.7305122954406613 1104 42-43 0.6828254244011092 1104 44-45 0.8670183478450113 1104 46-47 0.8464282327488988 1104 48-49 0.9889511794604005 1104 50-51 0.9317174349141943 1104 52-53 0.8095081194260842 1104 54-55 1.1668611179486774 1104 56-57 1.038105258627521 1104 58-59 0.8252783346704824 1104 60-61 1.1217945534711546 1104 62-63 1.0797839831750373 1104 64-65 1.0660723288986347 1104 66-67 1.1341899548048389 1104 68-69 1.639525252609193 1104 70-71 1.3708759623021187 1104 72-73 1.5505977133245175 1104 74-75 1.489675639900625 1104 76-77 1.570015685827446 1104 78-79 1.497456079461326 1104 80-81 1.8746171707455126 1104 82-83 1.7556866946811418 1104 84-85 1.663725863797179 1104 86-87 1.8896064315243883 1104 88-89 2.2087820414211627 1104 90-91 2.5206298716678077 1104 92-93 2.843282572118465 1104 94-95 2.8882853256150014 1104 96-97 2.9275575461371695 1104 98-99 3.272335976712732 1104 100-101 3.3754276262360285 1105 1 0.037638936578765936 1105 2 0.1685632749155701 1105 3 0.11496231600573381 1105 4 0.28205929318555434 1105 5 0.16657239981316252 1105 6 0.20841934173778043 1105 7 -0.054129416668814656 1105 8 0.13224383791742866 1105 9 -0.019957243580179806 1105 10-11 -0.05728003347690702 1105 12-13 0.07837653243318954 1105 14-15 0.21387465578748532 1105 16-17 0.28200626569313414 1105 18-19 0.371213527454735 1105 20-21 0.360718493013259 1105 22-23 0.3342679396605348 1105 24-25 0.2167172388503289 1105 26-27 0.34020526257339156 1105 28-29 0.21179273669705623 1105 30-31 0.6208889677411804 1105 32-33 0.5805626947222109 1105 34-35 0.5051527516378229 1105 36-37 0.30340863435841925 1105 38-39 0.3075693927400138 1105 40-41 0.10253825073986889 1105 42-43 0.0675454539861704 1105 44-45 0.3628664944278128 1105 46-47 0.3120045199477559 1105 48-49 0.4829191828415489 1105 50-51 0.39178692252078307 1105 52-53 0.49575805727259237 1105 54-55 0.5063458344044349 1105 56-57 0.515597147596516 1105 58-59 0.4730195213049946 1105 60-61 0.8415366786234308 1105 62-63 0.963561610403243 1105 64-65 1.129765675988608 1105 66-67 1.1084483082344683 1105 68-69 0.9843310850930926 1105 70-71 0.9896512898510359 1105 72-73 0.9889181394488737 1105 74-75 1.2437039632479667 1105 76-77 1.3318722417780187 1105 78-79 1.4232183920685415 1105 80-81 1.5484573865424416 1105 82-83 1.6001133908500016 1105 84-85 1.7495489625217893 1105 86-87 1.960713913562028 1105 88-89 2.1793865463064286 1105 90-91 2.2617325989631354 1105 92-93 2.18747852343985 1105 94-95 2.200835048410415 1105 96-97 2.5497769541872906 1105 98-99 2.680790871921939 1105 100-101 2.669213986030176 1106 1 -0.12847534841029074 1106 2 0.06364913279634621 1106 3 0.03196092538183848 1106 4 -0.038397020011231575 1106 5 0.06102096395319734 1106 6 0.0712053760301643 1106 7 -0.03060665993371714 1106 8 -0.07377337535931616 1106 9 -0.0707685012879935 1106 10-11 -0.3017615048186286 1106 12-13 -0.529582659010579 1106 14-15 -0.0645497328909812 1106 16-17 -0.142265016563627 1106 18-19 0.10973906725069327 1106 20-21 0.0016265867344245066 1106 22-23 -0.009037575151339183 1106 24-25 0.2527143633229585 1106 26-27 0.20306563269328848 1106 28-29 0.20094151894928558 1106 30-31 0.20938078445441022 1106 32-33 0.4590621444557854 1106 34-35 -0.21860987412117083 1106 36-37 0.04960254933636321 1106 38-39 0.46799109445027653 1106 40-41 0.3851898097072137 1106 42-43 0.24010247953775377 1106 44-45 0.5563050885016168 1106 46-47 0.7149894660361582 1106 48-49 0.9620448665951784 1106 50-51 0.8194649613603424 1106 52-53 0.9098217612636965 1106 54-55 0.9806795768869989 1106 56-57 0.8250114175371017 1106 58-59 0.727912087903551 1106 60-61 1.0236127695016997 1106 62-63 1.1211612702715037 1106 64-65 1.0452104852628175 1106 66-67 0.9332115667545331 1106 68-69 1.1524592364335895 1106 70-71 1.4905850203673445 1106 72-73 1.6439795027679516 1106 74-75 1.5921617667874415 1106 76-77 1.6158033741665 1106 78-79 1.5585123177435918 1106 80-81 1.5753257087828167 1106 82-83 1.5283643594820937 1106 84-85 1.8758450801972195 1106 86-87 2.242453498840632 1106 88-89 2.0040432870248743 1106 90-91 2.1000897953058093 1106 92-93 2.3940387508712213 1106 94-95 1.952119607771202 1106 96-97 2.025558432237368 1106 98-99 2.291621058069733 1106 100-101 2.0770620363464687 1107 1 0.12783343714377793 1107 2 0.08839514150461625 1107 3 0.11464899203216916 1107 4 0.1485302037084466 1107 5 0.017145971021605533 1107 6 0.2121672443505247 1107 7 0.1103759423696431 1107 8 0.16671632362095323 1107 9 0.05118784425326339 1107 10-11 0.18602288060726124 1107 12-13 0.3002818161369234 1107 14-15 0.20924157014560052 1107 16-17 -7.343773914811891E-4 1107 18-19 0.04626574811700124 1107 20-21 0.23101606518050488 1107 22-23 0.12957664082136944 1107 24-25 0.08042815896127564 1107 26-27 0.11752522970005685 1107 28-29 0.11312650377578137 1107 30-31 0.11675925504042795 1107 32-33 0.18864576837902547 1107 34-35 0.1025100436968529 1107 36-37 0.5179610776005532 1107 38-39 0.3802571303012172 1107 40-41 0.5216792636495029 1107 42-43 0.5613680064636597 1107 44-45 0.5617133594759238 1107 46-47 0.8204137587246052 1107 48-49 0.6144099543376242 1107 50-51 0.3333845323553888 1107 52-53 0.664178253827739 1107 54-55 0.7182778276927984 1107 56-57 0.6446686470999943 1107 58-59 0.828116600372212 1107 60-61 1.1593850161230002 1107 62-63 0.8463031095642854 1107 64-65 0.6926521944969828 1107 66-67 1.031736155373462 1107 68-69 0.9054402177164569 1107 70-71 0.7973362879676671 1107 72-73 1.0984556869611133 1107 74-75 0.8269317138215371 1107 76-77 0.8885136827258684 1107 78-79 1.0438472658143922 1107 80-81 1.121683596178439 1107 82-83 1.433949811765661 1107 84-85 1.4585274921249116 1107 86-87 1.7227529164042004 1107 88-89 2.0203095679137952 1107 90-91 2.2737185250666165 1107 92-93 2.373039938362975 1107 94-95 2.270809879763359 1107 96-97 2.739593925046446 1107 98-99 3.2667709082195806 1107 100-101 3.508965039004188 1108 1 -0.21703105330518824 1108 2 0.043284802194165195 1108 3 0.017832344552122237 1108 4 -0.15348048876584386 1108 5 -0.13994559792339345 1108 6 -0.15243344636770928 1108 7 -0.05502278155769602 1108 8 -0.09095474621182831 1108 9 -0.23944160687940297 1108 10-11 -0.12634126838494808 1108 12-13 -0.16989632672858335 1108 14-15 -0.13717410954844667 1108 16-17 0.031989374340199106 1108 18-19 -0.13181565517269433 1108 20-21 -0.03216123867697718 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0.313086560784658 2316 58-59 -0.021384592427612148 2316 60-61 0.7539686205472051 2316 62-63 0.7544972829178604 2316 64-65 0.34462754330056455 2316 66-67 0.5430117282788345 2316 68-69 1.055161897756939 2316 70-71 1.240004183579611 2316 72-73 0.9178856732258431 2316 74-75 1.1988864597190272 2316 76-77 1.1954536089891512 2316 78-79 1.312409761296209 2316 80-81 0.7876689211260306 2316 82-83 1.0917951815336089 2316 84-85 1.1815412996865398 2316 86-87 1.3541904209223823 2316 88-89 1.54695057131147 2316 90-91 1.9039487715785803 2316 92-93 2.5587227259000223 2316 94-95 2.620627345244454 2316 96-97 3.3483754619509476 2316 98-99 4.109203858411153 2316 100-101 3.8143037218640146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 33.0 5 56.0 6 111.0 7 300.0 8 612.0 9 1197.0 10 1949.0 11 3061.0 12 4283.0 13 5970.0 14 7957.0 15 11394.0 16 15153.0 17 19616.0 18 16383.0 19 11466.0 20 10604.0 21 11248.0 22 11953.0 23 13163.0 24 14926.0 25 17191.0 26 20344.0 27 23882.0 28 28129.0 29 32895.0 30 37962.0 31 44200.0 32 50949.0 33 59062.0 34 68624.0 35 76361.0 36 73977.0 37 52849.0 38 14597.0 39 397.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83857905827403 22.27344906578759 16.818052479540953 18.069919396397424 2 17.54425750296579 24.49607728759348 37.0052368374483 20.954428371992424 3 20.97645061708166 27.571457597346843 31.609655705793294 19.842436079778206 4 13.915437200553177 19.608318640912874 36.391628925170245 30.084615233363703 5 12.194421759255011 40.33849070009189 32.79500614138692 14.67208139926618 6 29.00578739357553 39.248490886329826 16.03258767934038 15.713134040754262 7 26.18130337608882 36.84911484994068 19.576465036408802 17.39311673756169 8 27.584303906982232 34.18704488998709 18.63645599155814 19.59219521147254 9 27.692186690961044 16.2007694677302 20.047452694775618 36.05959114653314 10-11 25.331284041081975 28.480596173634915 25.313849763719666 20.874270021563447 12-13 27.15062626299703 26.388649462486818 27.147021211467738 19.31370306304842 14-15 23.274104854725277 26.6668414463896 28.05398071742707 22.005072981458056 16-17 19.965852411632465 30.27776867466721 28.406140011666547 21.35023890203378 18-19 19.896049759787118 26.831352860597878 33.79713317559464 19.47546420402037 20-21 21.436899999934457 25.09685533931648 34.47302895861585 18.99321570213321 22-23 20.885207714909868 25.560964291636544 34.43073799207064 19.123090001382952 24-25 20.491305801157477 25.91723306220629 34.64053272859549 18.950928408040742 26-27 20.160371657449076 26.04748228033448 34.713634212620256 19.078511849596186 28-29 19.7008159624247 26.685491022709172 34.602732022313944 19.01096099255219 30-31 19.989080622496278 26.15800457224354 34.54790106755595 19.30501373770423 32-33 19.946609094150464 26.28804085656159 34.436806560540276 19.328543488747666 34-35 20.49222339470286 26.125526797008646 34.27447844638304 19.10777136190545 36-37 20.60987199570042 25.953740176833385 34.19654853742143 19.239839290044767 38-39 20.07394497974411 26.464201702923653 34.24192631850086 19.219926998831376 40-41 20.18076592844081 26.458285542002848 33.874997542160145 19.485950987396198 42-43 20.152675958845922 26.79885211171591 33.2803044604558 19.76816746898237 44-45 20.548852024932327 26.692927320036965 32.877573358326835 19.880647296703874 46-47 20.98864150275606 27.427985292286316 31.835776972334557 19.74759623262307 48-49 21.05923066335459 27.795809219193433 31.09894935539053 20.04601076206144 50-51 20.91051496660615 28.071677164373774 30.827013953976127 20.190793915043947 52-53 21.32421857078251 28.570782510667026 30.033098910029953 20.07190000852051 54-55 20.790375754556838 28.381233901148956 30.195709594751364 20.632680749542843 56-57 21.265295956689585 28.495933094322062 29.920628158324213 20.31814279066414 58-59 21.627483237532196 27.9675958393687 29.937210384537238 20.467710538561867 60-61 21.06971744673042 28.321000439134053 30.639693785925427 19.969588328210104 62-63 21.112123377006416 28.12712603147346 30.71926225478951 20.041488336730616 64-65 20.939222536097475 28.461392251577934 30.350455191940906 20.248930020383686 66-67 21.040944334842994 28.382479206674837 30.001573017506374 20.575003440975795 68-69 21.07817241582718 28.34302268422329 29.96690108997005 20.611903809979484 70-71 21.225839434238036 28.598965740989556 29.831883754006277 20.343311070766127 72-73 21.260211518697957 28.43121447597475 29.738983619622893 20.569590385704398 74-75 20.90205999750939 29.38370484948189 29.052322494805765 20.661912658202958 76-77 20.91169472973593 28.687251348534797 29.622017001697547 20.779036920031725 78-79 20.927293820007474 28.698196928683316 29.510988182705983 20.863521068603223 80-81 20.92676948083868 28.987435522667838 29.359323078133094 20.726471918360392 82-83 21.026321326114015 28.750669355668567 29.257642780036452 20.965366538180966 84-85 20.700544264770652 28.914262568426352 29.158080601522684 21.22711256528031 86-87 20.904681693353346 28.68888990843727 28.992023490394764 21.414404907814617 88-89 20.678363799623785 28.709076966435738 29.015946464970867 21.59661276896961 90-91 20.877852387707104 28.548327044948625 29.028805084545784 21.545015482798494 92-93 20.87472946950461 28.73862985398235 28.945878170499427 21.44076250601361 94-95 20.610068622888715 28.398340466530776 29.13982159359782 21.85176931698269 96-97 20.577559594423654 28.356065621046973 29.049635256565708 22.016739527963665 98-99 20.678562650543185 28.516279145979777 28.514378410263973 22.290779793213062 100-101 20.87392642179054 27.85698646875201 29.069043320708015 22.200043788749436 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 933.0 1 657.5 2 541.5 3 752.0 4 684.0 5 659.0 6 1209.5 7 2724.5 8 4179.5 9 4486.0 10 3823.0 11 3061.5 12 2869.0 13 2941.5 14 3062.5 15 3130.0 16 3215.0 17 3257.5 18 3290.5 19 3394.5 20 3576.5 21 3869.0 22 4220.5 23 4730.0 24 5340.0 25 6098.5 26 7010.0 27 8009.5 28 8976.0 29 10050.5 30 11442.0 31 12791.0 32 14418.5 33 15911.0 34 17158.5 35 18027.0 36 19166.0 37 20802.5 38 21624.0 39 21736.0 40 21804.5 41 22464.0 42 23075.0 43 23663.5 44 24717.0 45 25434.5 46 25094.5 47 26128.0 48 29295.0 49 28733.0 50 25228.0 51 22368.0 52 20091.0 53 19239.5 54 18844.0 55 17761.5 56 16051.5 57 14397.5 58 13219.0 59 12029.5 60 10348.0 61 8696.5 62 7378.5 63 5876.0 64 4458.0 65 3470.0 66 2542.0 67 1901.0 68 1360.5 69 888.0 70 649.5 71 498.5 72 399.5 73 361.5 74 305.5 75 241.5 76 172.0 77 113.5 78 84.0 79 56.0 80 31.0 81 18.5 82 9.0 83 2.5 84 1.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031984689296271296 2 0.0 3 0.0 4 0.0 5 0.0010486783375826653 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006095442837199243 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.554239609891658E-5 22-23 5.898815648902493E-4 24-25 0.0 26-27 0.001573017506373998 28-29 0.0030804926166490797 30-31 1.3108479219783316E-4 32-33 1.3108479219783316E-4 34-35 0.0 36-37 0.0 38-39 6.554239609891658E-5 40-41 0.0 42-43 0.009569189830441822 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.3108479219783316E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.898815648902493E-4 84-85 1.3108479219783316E-4 86-87 0.0 88-89 0.0 90-91 0.010880037752420153 92-93 0.001573017506373998 94-95 0.0 96-97 0.0 98-99 3.2771198049458294E-4 100-101 0.014681496726157315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 762865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.6449107581107 #Duplication Level Percentage of deduplicated Percentage of total 1 79.44358816404358 44.20631373692305 2 11.562753782034203 12.868168046386005 3 3.6721936655470273 6.130166664175913 4 1.6307876516048672 3.6298013335592705 5 0.9052585066695805 2.5186514408324685 6 0.5754638098621637 1.921297940658149 7 0.380358740669873 1.481551972644972 8 0.2780516557945342 1.2377727658265418 9 0.21309983952925116 1.0672129397855603 >10 1.2160964529400362 12.385525057012984 >50 0.08101425392792747 3.0847763080558512 >100 0.037082033990159205 3.9985787887707556 >500 0.002598104291957085 0.9859761395995958 >1k 9.447651970753037E-4 1.0594446362719732 >5k 4.723825985376519E-4 2.051072198692521 >10k+ 2.3619129926882594E-4 1.3736900308044488 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10452 1.3700982480517523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8657 1.1348010460566418 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6949 0.9109082209827427 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3021 0.396007157229654 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1944 0.2548288360325877 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1856 0.24329337431917836 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1240 0.16254514232531314 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 0.11968041527662168 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 803 0.10526108813486003 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 793 0.10395024021288171 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.554239609891658E-5 0.0 18-19 0.0 0.0 0.0 1.3108479219783316E-4 0.0 20-21 0.0 0.0 0.0 1.3108479219783316E-4 0.0 22-23 0.0 0.0 0.0 2.6216958439566633E-4 0.0 24-25 0.0 0.0 0.0 9.175935453848322E-4 0.0 26-27 0.0 0.0 0.0 0.0013108479219783318 0.0 28-29 0.0 0.0 0.0 0.0016385599024729145 0.0 30-31 0.0 0.0 0.0 0.0034082045971436624 0.0 32-33 0.0 0.0 0.0 0.007406290759177574 0.0 34-35 0.0 0.0 0.0 0.012387512862695235 0.0 36-37 0.0 0.0 0.0 0.019728261225773893 0.0 38-39 0.0 0.0 0.0 0.03637602983489871 0.0 40-41 0.0 0.0 0.0 0.05957803805391518 0.0 42-43 0.0 0.0 0.0 0.08068268959776631 0.0 44-45 0.0 0.0 0.0 0.10748952960222319 0.0 46-47 0.0 0.0 0.0 0.14425881381371541 0.0 48-49 0.0 0.0 0.0 0.1865992016936155 0.0 50-51 0.0 0.0 0.0 0.2407372208713206 0.0 52-53 0.0 0.0 0.0 0.29585837599050946 0.0 54-55 0.0 0.0 0.0 0.3594345002064585 0.0 56-57 0.0 0.0 0.0 0.44601600545312736 0.0 58-59 0.0 0.0 0.0 0.5308934084012243 0.0 60-61 0.0 0.0 0.0 0.6373342596658649 0.0 62-63 0.0 0.0 0.0 0.7527544191960569 0.0 64-65 0.0 0.0 0.0 0.8685022907067437 0.0 66-67 0.0 0.0 0.0 1.0045027626119956 0.0 68-69 0.0 0.0 0.0 1.1779934850858278 0.0 70-71 0.0 0.0 0.0 1.3849763719662063 0.0 72-73 0.0 0.0 0.0 1.6008074823199387 0.0 74-75 0.0 0.0 0.0 1.8153277447516927 0.0 76-77 0.0 0.0 0.0 2.075137802887798 0.0 78-79 0.0 0.0 0.0 2.3677846014694603 0.0 80-81 0.0 0.0 0.0 2.6932025981005814 0.0 82-83 0.0 0.0 0.0 3.0373657200159925 0.0 84-85 0.0 0.0 0.0 3.3539354931737595 0.0 86-87 0.0 0.0 0.0 3.687677374109443 0.0 88-89 0.0 0.0 0.0 4.080538496326349 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7740 0.0 28.298296 1 GTATCAA 10740 0.0 26.852499 1 AGTACTT 8020 0.0 24.313845 12-13 GTGTACG 105 0.0021924167 22.624605 1 GAGTACT 7950 0.0 22.406757 12-13 TAAGGTG 325 1.8189894E-12 21.921276 5 TACTTTT 8875 0.0 21.861584 14-15 GTACTTT 8355 0.0 21.289392 14-15 TATCAAC 13605 0.0 21.225748 2 GTACATG 6590 0.0 21.12431 1 AGAGTAC 12360 0.0 21.118551 10-11 TACATGG 6590 0.0 21.045313 2 ATCAACG 13840 0.0 20.796703 3 TAGACAG 275 1.2587407E-9 20.72557 5 TCAACGC 13940 0.0 20.647514 4 CAACGCA 14075 0.0 20.449474 5 GTACACG 140 4.8416512E-4 20.362144 1 AACGCAG 14415 0.0 20.131887 6 ATACAGT 335 3.45608E-11 19.849115 6 ATTCGCG 120 1.4151738E-7 19.79004 80-81 >>END_MODULE