FastQCFastQC Report
Thu 26 May 2016
SRR937158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748030
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111771.4941914094354505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74510.9960830447976685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61480.8218921701001297No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.23381415183883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17360.23207625362619147No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16610.2220499177840461No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12460.1665708594575084No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11440.1529350427121907No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC10070.1346202692405385No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10040.13421921580685267No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9810.1311444728152614No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.1148349665120383No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.11296338382150448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8320.11122548560886596No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7640.10213494111198748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG500.001609196938.0300142
GTATCAA80550.037.3107381
GGTATCA49400.035.4244541
ACGATAG300.00394514431.67679832-33
GGATAGG1751.4915713E-1029.8907571
ATCAACG101400.029.5331633
TATCAAC102500.029.2181822
TCAACGC103350.028.9837134
CAACGCA103800.028.9038685
AACGCAG106500.028.0981566
GTACATG61900.027.1953661
GTACGCT700.00836019627.1624743
TACATGG62150.026.9239042
ACATGGG63550.025.655823
ACGCAGA117850.025.271147
CGCAGAG118300.025.0947078
CATGGGA45050.025.0136764
GAGTACT64650.024.9435412-13
AGTACTT65050.024.13298212-13
GCAGAGT121300.023.9650029
Produced by FastQC (version 0.11.5)