##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937158_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 748030 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.324588586019274 33.0 31.0 34.0 31.0 34.0 2 32.438700319505905 34.0 31.0 34.0 31.0 34.0 3 32.46440116038127 34.0 31.0 34.0 31.0 34.0 4 36.04944053046001 37.0 35.0 37.0 35.0 37.0 5 36.07344090477655 37.0 37.0 37.0 35.0 37.0 6 36.16746387176985 37.0 37.0 37.0 35.0 37.0 7 36.13128216782749 37.0 37.0 37.0 35.0 37.0 8 36.13504271219069 37.0 37.0 37.0 35.0 37.0 9 37.84929080384477 39.0 38.0 39.0 35.0 39.0 10-11 37.762294961432026 39.0 38.0 39.0 35.0 39.0 12-13 37.65451987219764 39.0 38.0 39.0 35.0 39.0 14-15 38.89353702391615 40.0 38.0 41.0 36.0 41.0 16-17 38.90108484953812 40.0 38.0 41.0 36.0 41.0 18-19 38.95744889910833 40.0 38.5 41.0 36.0 41.0 20-21 38.947834311458095 40.0 39.0 41.0 36.0 41.0 22-23 38.813510821758484 40.0 38.5 41.0 35.5 41.0 24-25 38.672757108672116 40.0 38.0 41.0 35.0 41.0 26-27 38.51119005922222 40.0 38.0 41.0 35.0 41.0 28-29 38.51286579415264 40.0 38.0 41.0 35.0 41.0 30-31 38.25440557196904 40.0 38.0 41.0 35.0 41.0 32-33 37.95590016443191 40.0 37.0 41.0 34.5 41.0 34-35 37.80145515554189 40.0 37.0 41.0 34.0 41.0 36-37 37.77275710867211 40.0 37.0 41.0 34.0 41.0 38-39 37.66618517973878 40.0 37.0 41.0 34.0 41.0 40-41 37.44302367552103 40.0 37.0 41.0 33.0 41.0 42-43 37.1271406227023 40.0 36.0 41.0 33.0 41.0 44-45 36.744210125262356 40.0 36.0 41.0 32.5 41.0 46-47 36.171730411881875 40.0 36.0 41.0 30.5 41.0 48-49 35.669219148964615 39.5 35.0 41.0 27.5 41.0 50-51 34.721568653663624 38.5 34.5 40.0 25.5 40.5 52-53 34.77435998556207 38.5 35.0 40.0 26.0 40.5 54-55 35.06726334505301 39.0 35.0 40.5 26.0 41.0 56-57 34.87042431453284 39.0 35.0 41.0 24.5 41.0 58-59 34.64211261580418 39.0 35.0 40.5 24.0 41.0 60-61 34.36505688274534 38.0 35.0 40.0 22.5 41.0 62-63 34.056286512573024 38.0 34.5 40.0 22.0 41.0 64-65 33.70453056695587 37.0 34.0 40.0 20.0 41.0 66-67 33.325706188254486 37.0 34.0 39.5 17.5 41.0 68-69 32.87315682526102 36.0 33.5 39.0 11.5 41.0 70-71 32.44950670427657 35.5 33.0 39.0 7.5 40.5 72-73 32.071451679745465 35.0 33.0 38.5 4.0 40.0 74-75 31.641224282448565 35.0 32.5 37.5 2.0 39.5 76-77 31.235745892544415 35.0 32.0 37.0 2.0 39.0 78-79 30.79093285028675 35.0 32.0 36.5 2.0 39.0 80-81 30.338636819378902 35.0 31.0 36.0 2.0 37.5 82-83 29.892920070050668 35.0 31.0 35.5 2.0 37.0 84-85 29.49892250310816 34.0 30.5 35.0 2.0 37.0 86-87 29.122041896715373 34.0 30.0 35.0 2.0 36.0 88-89 28.753818028688688 34.0 29.5 35.0 2.0 36.0 90-91 28.382043500929107 34.0 29.0 35.0 2.0 35.5 92-93 27.995960055078008 34.0 29.0 35.0 2.0 35.0 94-95 27.545434675079875 34.0 28.0 35.0 2.0 35.0 96-97 27.038277208133366 34.0 26.5 35.0 2.0 35.0 98-99 26.41650535406334 33.0 25.0 35.0 2.0 35.0 100-101 24.68469580097055 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.002476566082997067 1101 2 -0.08158725669076006 1101 3 0.061317439658274964 1101 4 -0.014044758439275995 1101 5 0.03296827210829889 1101 6 0.0568780514694609 1101 7 -0.014527998432697586 1101 8 0.034071762614971135 1101 9 -0.016784875527157794 1101 10-11 0.08484686585843093 1101 12-13 -0.03567237736411499 1101 14-15 0.04350251657055537 1101 16-17 0.11353599751264909 1101 18-19 0.053560605265587924 1101 20-21 0.06327655590494885 1101 22-23 0.15213619993832594 1101 24-25 -0.023139847130202895 1101 26-27 0.08904487213551704 1101 28-29 -0.02118954700559783 1101 30-31 0.09782142650249881 1101 32-33 0.018266141601500863 1101 34-35 0.09505810936140335 1101 36-37 0.22030177043725274 1101 38-39 0.3377312729224613 1101 40-41 0.3768643830248166 1101 42-43 0.39751318624600884 1101 44-45 0.5306241227273745 1101 46-47 0.6126686421200702 1101 48-49 0.8613821762143132 1101 50-51 0.7418337170582276 1101 52-53 0.7620558448452002 1101 54-55 0.9353911386685994 1101 56-57 0.833746973886555 1101 58-59 0.9085006526741068 1101 60-61 0.6839664189662784 1101 62-63 0.8022523706650233 1101 64-65 0.949640656371713 1101 66-67 0.8881657366936153 1101 68-69 0.8168945141272488 1101 70-71 0.9357957264565471 1101 72-73 0.969785305525928 1101 74-75 0.8923046817447791 1101 76-77 0.9629832186637657 1101 78-79 0.9465645836481968 1101 80-81 0.8950459621136737 1101 82-83 1.0442938301411075 1101 84-85 0.9459547248303117 1101 86-87 0.9940678255903919 1101 88-89 1.0340772611496476 1101 90-91 1.0377450544508342 1101 92-93 1.111407089249898 1101 94-95 1.0627602629727768 1101 96-97 0.9431529210822909 1101 98-99 1.163858557765991 1101 100-101 1.5700740938192794 1102 1 0.013679568123215802 1102 2 -0.03427743448149556 1102 3 -0.00511945920503365 1102 4 -0.030204770097682854 1102 5 -0.050369676011783326 1102 6 -0.13536546354074375 1102 7 -0.06185093635166794 1102 8 -0.13888291532148855 1102 9 -0.00425937275828403 1102 10-11 0.08593401246292132 1102 12-13 0.04497320701361929 1102 14-15 0.07417870427093476 1102 16-17 0.08517291094896251 1102 18-19 0.17885862333611868 1102 20-21 -0.06990546099974893 1102 22-23 -0.08312830020740591 1102 24-25 -0.1323791359672768 1102 26-27 -0.14156610134160275 1102 28-29 -0.07346402966371812 1102 30-31 0.0034538951705300747 1102 32-33 -0.04806911051159091 1102 34-35 0.0106333789621047 1102 36-37 0.01847577218601515 1102 38-39 0.03407781911890595 1102 40-41 0.20853226410904568 1102 42-43 0.427623576802695 1102 44-45 0.24260678084453957 1102 46-47 0.34522729848847433 1102 48-49 0.3844822199333464 1102 50-51 0.23080668412322325 1102 52-53 0.3577670077714643 1102 54-55 0.39988472654385987 1102 56-57 0.5379013749358066 1102 58-59 0.5016407721727916 1102 60-61 0.6251042067832486 1102 62-63 0.5803917615131695 1102 64-65 0.4623373920172966 1102 66-67 0.5488951158833544 1102 68-69 0.5106190113583722 1102 70-71 0.5082873469753437 1102 72-73 0.49047460893601524 1102 74-75 0.577910190342859 1102 76-77 0.7351436675124994 1102 78-79 0.7256891100253462 1102 80-81 0.6557298006447105 1102 82-83 0.8461996156263893 1102 84-85 0.9161478172232016 1102 86-87 0.8595013726612173 1102 88-89 0.8306540609164799 1102 90-91 1.0118925333199655 1102 92-93 1.1083558651169874 1102 94-95 1.0547709741357032 1102 96-97 1.1158824460021393 1102 98-99 1.0313683945482701 1102 100-101 1.1912588795960168 1103 1 -0.02583795121328336 1103 2 -0.04954861144543088 1103 3 -0.11171483708221075 1103 4 -0.14841369750426736 1103 5 -0.2502753232021675 1103 6 -0.21370741676038563 1103 7 -0.16597683328578938 1103 8 -0.15652456102086632 1103 9 -0.09059801780534826 1103 10-11 -0.059561514514989256 1103 12-13 -0.07912403462275819 1103 14-15 -0.11755552366681599 1103 16-17 -0.11604027980678921 1103 18-19 -0.21215223306123931 1103 20-21 -0.2679240519977881 1103 22-23 -0.35293074173196004 1103 24-25 -0.25369238729521015 1103 26-27 -0.13169691394061545 1103 28-29 -0.16276430456819924 1103 30-31 -0.27532953601016885 1103 32-33 -0.3273388663591419 1103 34-35 -0.4388208890464682 1103 36-37 -0.3268030927846297 1103 38-39 -0.4632080815427244 1103 40-41 -0.16837226482581258 1103 42-43 -0.08707973602928831 1103 44-45 0.04713338600715389 1103 46-47 -0.07150634702452408 1103 48-49 -0.06615437031918958 1103 50-51 -0.03387333171873763 1103 52-53 -0.04859656505492893 1103 54-55 -0.15584167360524503 1103 56-57 -0.17347512772004592 1103 58-59 -0.20954935456283152 1103 60-61 -0.20746323800288735 1103 62-63 -0.4376488451404512 1103 64-65 -0.1569956151581735 1103 66-67 -0.13135952021766428 1103 68-69 -0.04441139121361459 1103 70-71 0.05263607961908434 1103 72-73 -0.03254281721252994 1103 74-75 -0.06241922909614672 1103 76-77 -0.017809225973660858 1103 78-79 0.06354497154803695 1103 80-81 0.08558547579148623 1103 82-83 0.02289854862424434 1103 84-85 0.05507941726047605 1103 86-87 -0.048731783614989865 1103 88-89 0.010704848354741614 1103 90-91 -0.13837305236636155 1103 92-93 -0.25064783910442756 1103 94-95 -0.10331948807657909 1103 96-97 -0.0314884886731015 1103 98-99 0.14323762854323974 1103 100-101 0.362707253466116 1104 1 0.09344542737419914 1104 2 0.03362779216322309 1104 3 0.06377201001714639 1104 4 0.03331895263733742 1104 5 0.01575340588603069 1104 6 -0.008284947343433657 1104 7 0.004388995449140509 1104 8 0.04043465184883388 1104 9 0.027028474851803708 1104 10-11 0.1743254522888975 1104 12-13 0.08535036028541754 1104 14-15 0.19246608154178801 1104 16-17 0.10522442779299013 1104 18-19 0.17354599472773913 1104 20-21 0.08037453782441162 1104 22-23 -0.05336832019682447 1104 24-25 -0.13833480649464747 1104 26-27 -0.06466894395433798 1104 28-29 -0.11052933332648252 1104 30-31 0.02078736568613948 1104 32-33 0.013386221959450495 1104 34-35 0.0872615611009735 1104 36-37 0.10824442407619017 1104 38-39 0.13691308280284176 1104 40-41 0.1472360385813829 1104 42-43 0.1737473201150621 1104 44-45 0.4309254650705441 1104 46-47 0.6825380604380342 1104 48-49 0.6251845686898818 1104 50-51 0.5631340549269126 1104 52-53 0.6854655791160482 1104 54-55 0.7314408144972973 1104 56-57 0.6345469008338611 1104 58-59 0.6070140304478215 1104 60-61 0.5579687931786808 1104 62-63 0.5754182915854855 1104 64-65 0.7028960668365087 1104 66-67 0.8208842098924052 1104 68-69 0.7546262281259644 1104 70-71 0.7439137065496837 1104 72-73 0.8168915058729276 1104 74-75 0.6498610692892974 1104 76-77 0.6197378529437394 1104 78-79 0.6665737152343532 1104 80-81 0.7454797124561061 1104 82-83 0.7332281740366398 1104 84-85 0.8328161986683185 1104 86-87 0.7346887734490366 1104 88-89 0.6090640203497202 1104 90-91 0.5747827679016595 1104 92-93 0.7253692844832038 1104 94-95 0.7486811834366627 1104 96-97 0.8137820873684731 1104 98-99 0.6921865643407337 1104 100-101 0.8907900101739479 1105 1 -0.08514842451921822 1105 2 -0.044599812137718686 1105 3 -0.04389795768027227 1105 4 -0.05488529674466491 1105 5 -0.05266589073660555 1105 6 0.010911582782895834 1105 7 0.027401931647752065 1105 8 0.03413792305403973 1105 9 0.004931536772666334 1105 10-11 0.06403677639147531 1105 12-13 -0.01998032413503381 1105 14-15 0.031915041944586164 1105 16-17 -0.10005658951087781 1105 18-19 -0.04708709039639558 1105 20-21 0.035584277128918984 1105 22-23 -0.0799970983099314 1105 24-25 -0.09462319424695664 1105 26-27 -0.11990332724798947 1105 28-29 -0.08775108373215801 1105 30-31 -0.17956561206602828 1105 32-33 -0.1508557289345589 1105 34-35 -0.06378182748544958 1105 36-37 -0.027490876732130687 1105 38-39 0.018297771681950792 1105 40-41 -0.021080132425147724 1105 42-43 0.002985707109097291 1105 44-45 0.05196650299953376 1105 46-47 0.08030190277716542 1105 48-49 0.16232571429415543 1105 50-51 0.13887792426069012 1105 52-53 0.1525779860375991 1105 54-55 0.21715867494406638 1105 56-57 0.03357668593827867 1105 58-59 0.06799956253050254 1105 60-61 0.162159787885912 1105 62-63 0.17135544562179206 1105 64-65 0.2335478813923899 1105 66-67 0.29439647122485013 1105 68-69 0.23828764246346168 1105 70-71 0.3471181834146648 1105 72-73 0.3299695022028679 1105 74-75 0.41127881902769303 1105 76-77 0.3527467702764291 1105 78-79 0.4779565842496538 1105 80-81 0.5755211143578656 1105 82-83 0.36533322567527904 1105 84-85 0.3375470863068948 1105 86-87 0.33089701136589866 1105 88-89 0.2826604503835384 1105 90-91 0.40960055403733264 1105 92-93 0.5229738887386546 1105 94-95 0.4415772692129103 1105 96-97 0.6517808437948673 1105 98-99 0.7996881946353973 1105 100-101 1.2212458102315757 1106 1 0.009970079315998248 1106 2 -0.032433131106948565 1106 3 -0.050005860808774116 1106 4 -0.03386831837700299 1106 5 -0.0058900569005260195 1106 6 0.05909912048866062 1106 7 0.021154409455213852 1106 8 -0.018424345324831393 1106 9 -0.05421092638341207 1106 10-11 -0.25843284353440055 1106 12-13 -0.36847985011969087 1106 14-15 -0.3573381684320438 1106 16-17 -0.2579222277079012 1106 18-19 0.008921269510530294 1106 20-21 -0.023272484053535436 1106 22-23 -0.2095140335852932 1106 24-25 -0.187561227514216 1106 26-27 -0.2193931143720107 1106 28-29 -0.14090931867139744 1106 30-31 -0.10429585424477494 1106 32-33 0.007752389655465208 1106 34-35 -0.17737572561524217 1106 36-37 -0.15696859801111174 1106 38-39 -0.14060811472673862 1106 40-41 -0.16436031858390265 1106 42-43 -0.13986097047428103 1106 44-45 0.27332261779743305 1106 46-47 0.39751555441898034 1106 48-49 0.4603546723222536 1106 50-51 0.3786370382829318 1106 52-53 0.1592120461944475 1106 54-55 0.16170146560165932 1106 56-57 0.21470182598826426 1106 58-59 0.21925311764556454 1106 60-61 0.21342153053867463 1106 62-63 0.3676908761139046 1106 64-65 0.39270241039350395 1106 66-67 0.5318294626925777 1106 68-69 0.42358885766642373 1106 70-71 0.6122461675566981 1106 72-73 0.5255715016857643 1106 74-75 0.42040016695495197 1106 76-77 0.6174097884613801 1106 78-79 0.6462324611780339 1106 80-81 0.7251238033176186 1106 82-83 0.548320815091806 1106 84-85 0.46488570562948084 1106 86-87 0.4036786195706732 1106 88-89 0.41543688751186636 1106 90-91 0.6106563154783373 1106 92-93 0.7128601250568494 1106 94-95 0.6651681508814455 1106 96-97 0.4931477316686923 1106 98-99 0.5897433527841542 1106 100-101 0.6325234970367148 1107 1 -0.1690998271229347 1107 2 -0.19867390718106748 1107 3 -0.10795763203242359 1107 4 -0.09318005833700482 1107 5 -0.10108510988781205 1107 6 -0.0865995990810049 1107 7 -0.04484223430010559 1107 8 0.020033621158702886 1107 9 -0.019909186193530104 1107 10-11 -0.0636308849587337 1107 12-13 -0.04858191673884704 1107 14-15 -0.005898891174048515 1107 16-17 0.03334188116316028 1107 18-19 -0.0689408486017058 1107 20-21 -0.1718051614632543 1107 22-23 0.038370382864904684 1107 24-25 0.06384954465193715 1107 26-27 0.09613894236232312 1107 28-29 0.18152326908519711 1107 30-31 0.21668159454950597 1107 32-33 0.20223389582773876 1107 34-35 0.19633430403925445 1107 36-37 0.19114661773105723 1107 38-39 0.03295617115174565 1107 40-41 0.18139403189080383 1107 42-43 0.3131263171742802 1107 44-45 0.3050841864970053 1107 46-47 0.3697240093970251 1107 48-49 0.41911975818234737 1107 50-51 0.4966780028584523 1107 52-53 0.3669612933381998 1107 54-55 0.482550181723127 1107 56-57 0.40181844399704403 1107 58-59 0.4082708413760514 1107 60-61 0.39944759890491355 1107 62-63 0.4932199444111873 1107 64-65 0.5967519724725392 1107 66-67 0.5282543724647653 1107 68-69 0.6954193902978645 1107 70-71 0.6378832793472284 1107 72-73 0.5405840301333846 1107 74-75 0.38203737069739674 1107 76-77 0.5312756766018829 1107 78-79 0.5239833174801873 1107 80-81 0.537374556173873 1107 82-83 0.5813692507599519 1107 84-85 0.5263573959354524 1107 86-87 0.5222363538756305 1107 88-89 0.5118332921922182 1107 90-91 0.5519749058555732 1107 92-93 0.7702496103560357 1107 94-95 0.7991558753015724 1107 96-97 0.7516599497786203 1107 98-99 0.8335326909602863 1107 100-101 1.0525699327508384 1108 1 -0.057501037500429675 1108 2 -0.027298342916964202 1108 3 -0.09727158609760522 1108 4 0.045977637977294705 1108 5 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-0.7925523586655423 2316 98-99 -1.1159838378210871 2316 100-101 -2.0655523837220926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2639.0 3 447.0 4 316.0 5 398.0 6 401.0 7 446.0 8 465.0 9 595.0 10 779.0 11 1199.0 12 1946.0 13 3149.0 14 3570.0 15 5370.0 16 8054.0 17 12921.0 18 10556.0 19 4989.0 20 4383.0 21 4066.0 22 4054.0 23 4141.0 24 4486.0 25 4725.0 26 5357.0 27 6373.0 28 7432.0 29 8874.0 30 11130.0 31 14666.0 32 19386.0 33 27932.0 34 42902.0 35 71272.0 36 109920.0 37 166068.0 38 149537.0 39 23076.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.75843172073223 19.15835017643343 15.305113820015102 20.778104282819232 2 14.824998022690133 20.91082265474293 41.129653883637666 23.134525438929277 3 18.809599768370198 27.061277341833573 30.45248237978172 23.676640510014504 4 11.462681605284818 16.654602097090294 38.13690873555268 33.74580756207221 5 12.157135062744903 38.91001760155076 33.32886480051799 15.603982535186345 6 29.245788503579234 37.266920999614214 17.750841227656565 15.736449269149984 7 26.754315471406986 35.47874677210239 20.22244366582018 17.544494090670437 8 27.135580121005603 32.7312521012568 19.721341536104113 20.41182624163348 9 27.88786724081354 15.889849520837378 20.658840232248213 35.56344300610087 10-11 24.507423396509516 27.489642745903147 26.239739034037434 21.7631948235499 12-13 26.355118779473845 25.39976598410916 27.820698551499234 20.424416684917762 14-15 23.034024667281027 25.55486648378732 28.360675939221192 23.050432909710462 16-17 20.0631288354652 29.606995205396423 28.362635176812308 21.96724078232607 18-19 19.86256371379339 26.417876762739912 33.52810505752407 20.19145446594263 20-21 21.362085845037505 25.018716409235125 33.612729033269 20.006468712458364 22-23 20.55873800776557 25.32013891772219 33.91262594943406 20.208497125078186 24-25 20.219580365261947 25.463876244313287 34.034822692470975 20.28172069795379 26-27 19.83897082752442 25.814420039185908 34.08249932703627 20.264109806253405 28-29 19.587462470255815 26.30889482563361 33.97343466727031 20.130208036840262 30-31 19.692483075919412 25.923933901811868 33.973145183058705 20.410437839210015 32-33 19.52376870104976 26.006866260620537 33.88511671705407 20.58424832127563 34-35 20.19219240942462 26.07966735053246 33.55852009031583 20.16962014972709 36-37 20.096735397950383 25.825592358666942 33.607012948052436 20.470659295330236 38-39 19.80904523959077 26.255944735886988 33.47353378806405 20.46147623645819 40-41 19.744733735497224 26.450076703086406 33.54660206331637 20.2585874981 42-43 19.711220055755074 26.47689673391517 32.887123095766 20.924760114563753 44-45 19.798290289204314 26.655750769302887 32.771064166055574 20.77489477543722 46-47 20.28746643914376 27.063996844325178 31.94739023950849 20.701146477022576 48-49 20.287319638241684 27.301125689113693 31.332897087219973 21.078657585424647 50-51 20.207881353037273 27.76029208951035 31.068670640409334 20.963155917043043 52-53 20.465752855687224 27.908392916837215 30.579350622988994 21.046503604486567 54-55 20.035604802763725 27.827135869561577 30.78737114524094 21.34988818243376 56-57 20.485815374890855 28.024946298766345 30.320037925659282 21.16920040068352 58-59 20.6959980447236 27.532970178686817 30.52012361096696 21.250908165622626 60-61 20.38709876134021 27.640011275761335 31.243768702262987 20.729121260635473 62-63 20.317472009428403 27.73102266030964 31.131341404617775 20.820163925644188 64-65 20.074926429477756 27.96309253983187 30.98385298617644 20.978128044513934 66-67 20.093564322626175 28.10478347097256 30.483328624094252 21.31832358230701 68-69 20.202100684761366 27.88372113265837 30.717241831842106 21.19693635073816 70-71 20.350766292632915 28.00974179684858 30.53559124095412 21.103900669564386 72-73 20.31163369982992 27.994335150192178 30.512782740287392 21.181248409690507 74-75 20.037446286476147 28.652101096937805 30.16428285874808 21.146169757837974 76-77 20.066308228126555 28.164657620658275 30.334909453978327 21.434124697236847 78-79 20.05445460353004 28.27768901412525 30.390182100158437 21.277674282186272 80-81 20.257598616783554 28.55495533140342 29.940381603274822 21.2470644485382 82-83 20.071004427397906 28.42199289152206 30.083673267141105 21.423329413938937 84-85 20.072098082316888 28.342822096241033 29.883409741141904 21.701670080300175 86-87 19.987961560015773 28.42283714829577 29.851648656990832 21.737552634697625 88-89 20.03588837109647 28.37156516149767 29.583065509668433 22.009480957737427 90-91 19.93705701362314 28.33264698693933 29.744176936752343 21.986119062685187 92-93 20.087512261425307 28.3036883036883 29.91673948195687 21.69205995292952 94-95 20.055987637458287 28.007611426245226 29.867482980079597 22.06891795621689 96-97 19.965371002968773 28.047813726527338 29.91196789385797 22.074847376645916 98-99 20.086338276769954 28.125895590547383 29.393917885562608 22.393848247120047 100-101 20.053121828022935 27.772525556594097 29.925251816479953 22.249100798903015 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1475.0 1 925.5 2 637.0 3 888.5 4 704.5 5 663.0 6 1300.5 7 3068.0 8 5110.0 9 5496.5 10 4178.5 11 2945.5 12 2637.0 13 2650.5 14 2725.0 15 2681.0 16 2652.5 17 2739.5 18 2786.5 19 2910.0 20 3096.0 21 3325.0 22 3697.5 23 4337.0 24 4904.5 25 5494.0 26 6469.5 27 7508.0 28 8311.5 29 9352.5 30 10642.5 31 11914.0 32 13760.5 33 15285.0 34 16728.0 35 17606.0 36 18692.5 37 20366.5 38 21029.5 39 21182.0 40 21065.0 41 21798.5 42 22454.5 43 23034.5 44 24264.0 45 25125.0 46 24926.0 47 26041.0 48 29717.0 49 28908.5 50 24575.0 51 21796.5 52 19957.5 53 19385.5 54 19155.5 55 18053.0 56 16339.5 57 14547.5 58 13286.5 59 12195.0 60 10451.5 61 8857.0 62 7596.5 63 5817.0 64 4169.0 65 3245.5 66 2352.5 67 1762.0 68 1282.5 69 822.0 70 631.0 71 492.0 72 392.5 73 374.0 74 293.5 75 215.0 76 160.0 77 103.5 78 62.5 79 37.0 80 26.5 81 22.0 82 15.5 83 8.0 84 9.0 85 10.0 86 7.0 87 5.0 88 5.5 89 5.0 90 4.0 91 4.0 92 2.5 93 2.5 94 4.0 95 3.5 96 3.5 97 4.0 98 3.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.323917489940243 2 0.27632581580952637 3 0.26897316952528644 4 0.2732510728179351 5 0.27712792267689795 6 0.2007940857986979 7 0.189029851743914 8 0.1942435463818296 9 0.1891635362218093 10-11 0.21021884149031458 12-13 0.20045987460395975 14-15 0.1943772308597249 16-17 0.19537986444393943 18-19 0.19110196115129072 20-21 0.18167720545967406 22-23 0.18729195353127548 24-25 0.17820140903439702 26-27 0.17820140903439702 28-29 0.19457775757656778 30-31 0.20513883133029426 32-33 0.19798671176289723 34-35 0.2056735692418753 36-37 0.18100878307019772 38-39 0.1819445744154646 40-41 0.17639666858281086 42-43 0.18334826143336497 44-45 0.18882932502707112 46-47 0.19230512145234818 48-49 0.20179671938291244 50-51 0.2067430450650375 52-53 0.2230525513682606 54-55 0.20072724355975025 56-57 0.17459192813122468 58-59 0.17813456679544937 60-61 0.17318824111332431 62-63 0.18047404515861662 64-65 0.17345561006911486 66-67 0.16991297140489017 68-69 0.17024718259962837 70-71 0.18054088739756427 72-73 0.17646351082175848 74-75 0.18187773217651698 76-77 0.18254615456599335 78-79 0.18087509859230244 80-81 0.18241247008809808 82-83 0.1759287729101774 84-85 0.16971244468804728 86-87 0.16784086199751347 88-89 0.1697792869269949 90-91 0.17679772201649666 92-93 0.17078192051120944 94-95 0.16817507319225164 96-97 0.1680413887143564 98-99 0.1759287729101774 100-101 0.16790770423646112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 748030.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.14574133676881 #Duplication Level Percentage of deduplicated Percentage of total 1 78.0031940460776 42.235407682607025 2 12.298441713193885 13.31816487695848 3 3.9572116946653515 6.427984825025601 4 1.7807810867102691 3.856868483936972 5 0.9879461999499954 2.6746539698568066 6 0.6244983434560396 2.0288355462006797 7 0.4028384280900868 1.526838972951347 8 0.2974630932066099 1.28850877616002 9 0.2339167382341893 1.1399035682492165 >10 1.2859599286107082 12.905532811453613 >50 0.08080058551573521 3.0194215159200746 >100 0.04299461451798859 4.328428514084557 >500 0.0014825729143072045 0.6573185363761533 >1k 0.001729668400025072 1.2773091933204024 >5k 4.94190971435735E-4 1.819433091019603 >10k+ 2.470954857178675E-4 1.4953896358795575 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11177 1.4941914094354505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7451 0.9960830447976685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6148 0.8218921701001297 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1749 0.23381415183883 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1736 0.23207625362619147 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1661 0.2220499177840461 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1246 0.1665708594575084 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1144 0.1529350427121907 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1007 0.1346202692405385 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1004 0.13421921580685267 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 981 0.1311444728152614 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 859 0.1148349665120383 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.11296338382150448 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 832 0.11122548560886596 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 764 0.10213494111198748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.6736895579054315E-4 0.0 20-21 0.0 0.0 0.0 2.6736895579054315E-4 0.0 22-23 0.0 0.0 0.0 5.347379115810863E-4 0.0 24-25 0.0 0.0 0.0 7.352646284239936E-4 0.0 26-27 0.0 0.0 0.0 0.0010694758231621726 0.0 28-29 0.0 0.0 0.0 0.0018047404515861662 0.0 30-31 0.0 0.0 0.0 0.005481063593706134 0.0 32-33 0.0 0.0 0.0 0.009291071213721375 0.0 34-35 0.0 0.0 0.0 0.013769501223212972 0.0 36-37 0.0 0.0 0.0 0.021456358702191092 0.0 38-39 0.0 0.0 0.0 0.03502533320856115 0.0 40-41 0.0 0.0 0.0 0.05400852906968971 0.0 42-43 0.0 0.0 0.0 0.07319225164766119 0.0 44-45 0.0 0.0 0.0 0.10166704543935404 0.0 46-47 0.0 0.0 0.0 0.13642500969212465 0.0 48-49 0.0 0.0 0.0 0.1749929815649105 0.0 50-51 0.0 0.0 0.0 0.2269294012272235 0.0 52-53 0.0 0.0 0.0 0.2796010855179605 0.0 54-55 0.0 0.0 0.0 0.33641698862345093 0.0 56-57 0.0 0.0 0.0 0.41836557357325244 0.0 58-59 0.0 0.0 0.0 0.5061962755504459 0.0 60-61 0.0 0.0 0.0 0.6057912115824231 0.0 62-63 0.0 0.0 0.0 0.7166156437576032 0.0 64-65 0.0 0.0 0.0 0.8270390224990976 0.0 66-67 0.0 0.0 0.0 0.9603892891996311 0.0 68-69 0.0 0.0 0.0 1.130502787321364 0.0 70-71 0.0 0.0 0.0 1.338248465970616 0.0 72-73 0.0 0.0 0.0 1.552678368514632 0.0 74-75 0.0 0.0 0.0 1.768645642554443 0.0 76-77 0.0 0.0 0.0 2.017432455917543 0.0 78-79 0.0 0.0 0.0 2.307995668622916 0.0 80-81 0.0 0.0 0.0 2.6420731788831997 0.0 82-83 0.0 0.0 0.0 3.003622849350962 0.0 84-85 0.0 0.0 0.0 3.338569308717565 0.0 86-87 0.0 0.0 0.0 3.7276579816317525 0.0 88-89 0.0 0.0 0.0 4.160795690012433 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 50 0.0016091969 38.030014 2 GTATCAA 8055 0.0 37.310738 1 GGTATCA 4940 0.0 35.424454 1 ACGATAG 30 0.003945144 31.676798 32-33 GGATAGG 175 1.4915713E-10 29.890757 1 ATCAACG 10140 0.0 29.533163 3 TATCAAC 10250 0.0 29.218182 2 TCAACGC 10335 0.0 28.983713 4 CAACGCA 10380 0.0 28.903868 5 AACGCAG 10650 0.0 28.098156 6 GTACATG 6190 0.0 27.195366 1 GTACGCT 70 0.008360196 27.162474 3 TACATGG 6215 0.0 26.923904 2 ACATGGG 6355 0.0 25.65582 3 ACGCAGA 11785 0.0 25.27114 7 CGCAGAG 11830 0.0 25.094707 8 CATGGGA 4505 0.0 25.013676 4 GAGTACT 6465 0.0 24.94354 12-13 AGTACTT 6505 0.0 24.132982 12-13 GCAGAGT 12130 0.0 23.965002 9 >>END_MODULE