FastQCFastQC Report
Thu 26 May 2016
SRR937158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748030
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108141.4456639439594667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88331.180834993248934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69270.9260323783805462No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29190.39022499097629776No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.26710158683475266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.24892049784099568No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14130.18889616726601874No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.12258866622996403No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.11443391307835248No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7570.10119914976672059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT2800.030.5390664
GGACCGA1354.2226748E-728.1474746
GGTATCA77650.027.352341
GATAGTC1754.94947E-927.1458387
GTATCAA107400.026.8542371
GGTTGCG908.938882E-426.3917877
GTTGCGG908.938882E-426.3917878
GTATTCG458.950063E-426.38825866-67
CGACCGT458.950063E-426.38825818-19
CGATCCG458.950063E-426.38825870-71
TAAGGTG3250.026.310585
AGTACTT80450.023.02925312-13
GGTAAGG3350.022.6890583
GTGCTAA1903.2739445E-722.5069851
GTACATG63200.022.4041041
TACATGG63800.021.8911192
TCGACCG550.00287816821.59039118-19
GAGTACT80450.021.55301912-13
GTCGGGA1553.900772E-521.4539682
TATCAAC135950.021.2104932
Produced by FastQC (version 0.11.5)