##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937158_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 748030 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28248332286138 31.0 31.0 33.0 30.0 34.0 2 31.52292421426948 31.0 31.0 34.0 30.0 34.0 3 31.50682860313089 31.0 31.0 34.0 30.0 34.0 4 35.34982153122201 37.0 35.0 37.0 33.0 37.0 5 35.1401294065746 35.0 35.0 37.0 32.0 37.0 6 35.325309145355135 37.0 35.0 37.0 33.0 37.0 7 35.2672767135008 37.0 35.0 37.0 32.0 37.0 8 35.26292127321097 37.0 35.0 37.0 32.0 37.0 9 36.75252997874417 39.0 37.0 39.0 32.0 39.0 10-11 36.6350353595444 38.0 35.0 39.0 32.0 39.0 12-13 36.50451185112897 38.0 35.0 39.0 32.0 39.0 14-15 37.27531449273425 39.0 36.0 40.0 32.0 41.0 16-17 37.272036549336256 39.0 36.0 40.0 32.0 41.0 18-19 37.40258813149205 39.0 36.0 40.0 32.0 41.0 20-21 37.34853949707899 39.0 36.0 40.0 32.0 41.0 22-23 37.156323944226834 39.0 36.0 40.0 31.0 41.0 24-25 36.943298397123115 39.0 36.0 40.0 31.0 41.0 26-27 36.56758485622234 38.0 35.0 40.0 30.0 41.0 28-29 36.46064730024197 38.0 35.0 40.0 30.0 41.0 30-31 36.41335240565218 38.0 35.0 40.0 30.0 41.0 32-33 36.17761119206449 38.0 35.0 40.0 30.0 41.0 34-35 35.92034677753566 38.0 35.0 40.0 30.0 41.0 36-37 35.60496570993142 38.0 34.5 40.0 29.5 41.0 38-39 35.34277635923693 38.0 34.0 40.0 28.5 41.0 40-41 35.012184671737764 38.0 34.0 40.0 27.0 41.0 42-43 34.58713353742497 38.0 33.5 40.0 25.5 41.0 44-45 33.97987981765437 37.0 33.0 40.0 23.5 41.0 46-47 33.253729128510884 37.0 33.0 40.0 19.5 41.0 48-49 32.609845193374596 37.0 31.5 40.0 13.5 41.0 50-51 32.22444286993837 37.0 31.0 39.5 8.5 40.5 52-53 31.870739809900673 36.0 31.0 39.0 7.5 40.0 54-55 31.57019504565325 36.0 30.5 39.0 6.0 40.0 56-57 31.21654211729476 35.5 30.0 39.0 2.0 40.0 58-59 30.87479178642568 35.0 29.0 39.0 2.0 40.0 60-61 30.40866409101239 35.0 28.5 38.0 2.0 40.0 62-63 29.92417616940497 34.5 28.0 38.0 2.0 40.0 64-65 29.4733613625122 34.0 28.0 38.0 2.0 40.0 66-67 28.954895525580525 34.0 26.5 37.0 2.0 39.0 68-69 28.391974920791945 33.5 26.0 36.5 2.0 39.0 70-71 27.942932770076066 33.0 26.0 36.0 2.0 39.0 72-73 27.427682044837773 32.5 25.0 35.5 2.0 38.5 74-75 26.90082683849578 32.0 24.0 35.0 2.0 38.0 76-77 25.965786799994653 30.5 23.0 34.0 2.0 36.0 78-79 26.579502827426708 32.0 24.0 35.0 2.0 37.0 80-81 26.326097215352327 32.0 23.5 35.0 2.0 36.0 82-83 25.87768939748406 32.0 22.5 35.0 2.0 36.0 84-85 25.384699811504888 31.5 20.0 34.0 2.0 35.5 86-87 24.891974920791945 31.0 18.5 34.0 2.0 35.0 88-89 24.288432282127722 31.0 16.0 34.0 2.0 35.0 90-91 23.540811865834257 30.0 7.0 34.0 2.0 35.0 92-93 22.797160541689504 29.5 2.0 34.0 2.0 35.0 94-95 22.02707378046335 29.0 2.0 34.0 2.0 35.0 96-97 21.201766640375386 29.0 2.0 34.0 2.0 35.0 98-99 20.09665788805262 28.0 2.0 33.0 2.0 34.5 100-101 18.378438698982663 25.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.007243674636658426 1101 2 0.15726764274978677 1101 3 0.10494196000913192 1101 4 0.09745775808234924 1101 5 -0.18339483713443627 1101 6 -0.057236255697851846 1101 7 -0.02808599816476942 1101 8 -0.1676481111075887 1101 9 -0.08182243452573346 1101 10-11 0.016949575368691683 1101 12-13 0.2254660725580706 1101 14-15 0.23027384717234156 1101 16-17 0.1481411710662357 1101 18-19 0.2583300846771337 1101 20-21 -0.10811502743682411 1101 22-23 -0.12150488912083546 1101 24-25 0.026745980159653016 1101 26-27 -0.19429207303625162 1101 28-29 0.04170565136467275 1101 30-31 0.07651784634129655 1101 32-33 0.1854396047867155 1101 34-35 0.07904031993555094 1101 36-37 0.15544073956386484 1101 38-39 0.19676726741498385 1101 40-41 0.141856938634632 1101 42-43 0.07653521977206168 1101 44-45 0.10611390842785084 1101 46-47 0.06821111359522547 1101 48-49 0.4094219393513683 1101 50-51 0.1363122800399026 1101 52-53 0.2835459344572264 1101 54-55 0.30964855574828576 1101 56-57 0.4243495017447003 1101 58-59 0.3228396066464505 1101 60-61 0.3390457290079887 1101 62-63 0.17787578261437176 1101 64-65 0.362427213052662 1101 66-67 0.4138917452893587 1101 68-69 0.09851475848319069 1101 70-71 -0.15463292933512562 1101 72-73 -0.35263253409167916 1101 74-75 -0.15505317071783153 1101 76-77 0.2243697304842982 1101 78-79 0.21521911442221153 1101 80-81 0.1669871804733596 1101 82-83 -0.04390144112711525 1101 84-85 0.10545875987711284 1101 86-87 0.016322345031991148 1101 88-89 0.06872330989398634 1101 90-91 0.38616625833759954 1101 92-93 0.4522177827542464 1101 94-95 0.08533460645431035 1101 96-97 0.1165345358138481 1101 98-99 0.2966105576604825 1101 100-101 0.7523483828634134 1102 1 0.21069205838578497 1102 2 0.07709713852361233 1102 3 -0.09295151128203827 1102 4 0.0107272130517444 1102 5 -0.14619795575874406 1102 6 0.03675456330535809 1102 7 0.16412909947440824 1102 8 0.20788234648495063 1102 9 0.23450298080507537 1102 10-11 0.12259989597493615 1102 12-13 0.12267133341496361 1102 14-15 0.27255707363693205 1102 16-17 0.09272438295781171 1102 18-19 0.14059545920351013 1102 20-21 -0.059246257398939406 1102 22-23 0.10216058741661982 1102 24-25 0.0800190783817456 1102 26-27 0.029548279630610352 1102 28-29 -0.040486129457242725 1102 30-31 -0.10390877854941749 1102 32-33 -0.10126944505298496 1102 34-35 0.05357398359046073 1102 36-37 0.13762013332661382 1102 38-39 -0.053197393033862284 1102 40-41 -0.2394823210564212 1102 42-43 0.011512995930615944 1102 44-45 -0.010561623487042482 1102 46-47 -0.14652730086120158 1102 48-49 -0.18359248792930316 1102 50-51 -0.17085873424164788 1102 52-53 -0.4108977408560932 1102 54-55 -0.3043273259188659 1102 56-57 -0.1204745291500835 1102 58-59 -0.24119456706383602 1102 60-61 -0.1754274566521694 1102 62-63 -0.24749072860684507 1102 64-65 0.05788298396493019 1102 66-67 -0.11364760183975875 1102 68-69 -0.02950586232706698 1102 70-71 0.025730302958667295 1102 72-73 0.11023834903277319 1102 74-75 -0.15828655748553544 1102 76-77 0.014199226258124753 1102 78-79 0.013857995214983987 1102 80-81 -0.37553905094605255 1102 82-83 0.0927977719303037 1102 84-85 0.08110798750754 1102 86-87 0.14778037476093786 1102 88-89 0.4674266762594783 1102 90-91 0.28543323589516234 1102 92-93 0.2943680304954306 1102 94-95 0.35569383117013587 1102 96-97 0.5436239412350083 1102 98-99 0.5358917439035586 1102 100-101 0.6394465320910463 1103 1 0.017228028938390594 1103 2 -0.12727570650480757 1103 3 -0.024778694210002783 1103 4 0.015497271760160913 1103 5 -0.09319147919551796 1103 6 -0.15013971495389455 1103 7 -0.05803099744107243 1103 8 -0.10054650450749847 1103 9 -0.22012030686615702 1103 10-11 -0.0681937159904038 1103 12-13 -4.73643753991837E-4 1103 14-15 -0.11197430740864434 1103 16-17 -0.3543215587065305 1103 18-19 -0.3582862852750992 1103 20-21 -0.2524394593372463 1103 22-23 -0.3124355592612318 1103 24-25 -0.11735302114299628 1103 26-27 -0.12145989761288689 1103 28-29 -0.3855902670026765 1103 30-31 -0.08289198141959986 1103 32-33 -0.048236759375974714 1103 34-35 -0.002927041477100545 1103 36-37 -0.024975384332499573 1103 38-39 -0.044248436739017905 1103 40-41 -0.15073243754136456 1103 42-43 0.03310056931613303 1103 44-45 -0.15745788199044597 1103 46-47 -8.104524780137012E-4 1103 48-49 -0.16484423520356017 1103 50-51 -0.04325820772967148 1103 52-53 0.17684163571645328 1103 54-55 0.11177730086335558 1103 56-57 0.017504806419758268 1103 58-59 -0.1533154085511157 1103 60-61 -0.3552685711801722 1103 62-63 0.02321447128132803 1103 64-65 0.17398062165014494 1103 66-67 0.33811015605791894 1103 68-69 0.2636663723202126 1103 70-71 0.12249726316740706 1103 72-73 0.21635031436685281 1103 74-75 0.23905234382841556 1103 76-77 -0.07155116979070542 1103 78-79 0.07481456962556621 1103 80-81 0.09357496452314962 1103 82-83 -0.12737625658521523 1103 84-85 -0.28679807902866017 1103 86-87 -0.12374604402575784 1103 88-89 -0.11424383441054076 1103 90-91 -0.26201690854617254 1103 92-93 -0.030001785924287816 1103 94-95 -0.1438052835442427 1103 96-97 0.1095689836355298 1103 98-99 -0.06028696730695415 1103 100-101 -0.15789586377141163 1104 1 -0.15641726608949114 1104 2 0.042443425783300626 1104 3 0.03757276998082304 1104 4 0.02008034962649674 1104 5 -0.26647766062087186 1104 6 -0.12028803179136105 1104 7 -0.1695854046116665 1104 8 -0.07504286957713191 1104 9 -0.25230914806787297 1104 10-11 -0.09482286550428398 1104 12-13 0.0019047539570280492 1104 14-15 -0.13295508169260017 1104 16-17 -0.17678440650659155 1104 18-19 -0.0842377173500779 1104 20-21 -0.2795696891115682 1104 22-23 -0.17914893989245684 1104 24-25 -0.1963404959612447 1104 26-27 -0.18185120005662014 1104 28-29 -0.31322997095292493 1104 30-31 -0.18600612407237094 1104 32-33 -0.2789088565410154 1104 34-35 -0.30642219490327705 1104 36-37 -0.14739735741358118 1104 38-39 -0.2913488863609288 1104 40-41 -0.2522623198805718 1104 42-43 -0.02063946747111345 1104 44-45 -0.025985443229529892 1104 46-47 -0.0917085284465955 1104 48-49 0.11188564129639644 1104 50-51 -0.039439706080084846 1104 52-53 0.1374880402009957 1104 54-55 0.3452416522691557 1104 56-57 0.05224101850115659 1104 58-59 0.3336404467523799 1104 60-61 0.4743557463580039 1104 62-63 0.5614097064569457 1104 64-65 0.22648894257507735 1104 66-67 0.11534366748824354 1104 68-69 -0.04456075973432405 1104 70-71 -0.04008448627056893 1104 72-73 0.011111416232491678 1104 74-75 0.07054266527888231 1104 76-77 0.22224937617875895 1104 78-79 0.4106332318544048 1104 80-81 0.47842723161024026 1104 82-83 0.3631061380893925 1104 84-85 0.3878110564710333 1104 86-87 0.34525303629306237 1104 88-89 0.465026843817828 1104 90-91 0.45366329057209853 1104 92-93 0.5139363472689027 1104 94-95 0.23399135013068673 1104 96-97 0.06796362904734465 1104 98-99 0.3464945865162967 1104 100-101 0.5540413789368337 1105 1 0.028546867427849065 1105 2 0.05902390167768701 1105 3 -0.01898556423716613 1105 4 0.12800358170463255 1105 5 0.03974703189796713 1105 6 0.01780095954912042 1105 7 0.0507644642064804 1105 8 -0.15280185894609133 1105 9 -0.04609203831491726 1105 10-11 -0.07092502052635297 1105 12-13 0.0168261206747502 1105 14-15 0.03487162177574987 1105 16-17 -0.09917745159371805 1105 18-19 -0.1051358201420527 1105 20-21 -0.08497203066966819 1105 22-23 -0.17187403429789327 1105 24-25 -0.03173608885771273 1105 26-27 -0.03570324512858036 1105 28-29 -0.36023781153285483 1105 30-31 -0.2909413672516692 1105 32-33 -0.08483744205187094 1105 34-35 0.14699573682150202 1105 36-37 0.025389991499814357 1105 38-39 0.05072674414605416 1105 40-41 0.29835832950750074 1105 42-43 0.04950642494460311 1105 44-45 0.3325258323433431 1105 46-47 0.504856478605376 1105 48-49 0.49819571575915944 1105 50-51 0.5908356241493706 1105 52-53 0.5113047495839105 1105 54-55 0.360844330502065 1105 56-57 0.6618252554474289 1105 58-59 0.6379516282989606 1105 60-61 0.5414181070246791 1105 62-63 0.6707319588417242 1105 64-65 0.7858965010761167 1105 66-67 0.7099371554088982 1105 68-69 0.7903108640391636 1105 70-71 0.36752292360825933 1105 72-73 0.40723965596922085 1105 74-75 0.4950107342517178 1105 76-77 0.467415742062375 1105 78-79 0.48993379752874233 1105 80-81 0.5290092088922727 1105 82-83 0.5322067462272209 1105 84-85 0.7464868780637168 1105 86-87 1.0807908060499614 1105 88-89 1.2527184982256934 1105 90-91 0.9133980454212782 1105 92-93 0.9035244514257741 1105 94-95 0.6459759116193382 1105 96-97 0.4471299797654318 1105 98-99 0.7087961076191327 1105 100-101 0.4071461424303635 1106 1 -0.2382763679423121 1106 2 -0.14083303187398144 1106 3 -0.002644962668604478 1106 4 0.10119413587165837 1106 5 -0.07595321803739097 1106 6 -0.047314096901793334 1106 7 -0.1770636836863062 1106 8 -0.1381099689176537 1106 9 -0.22353494101249538 1106 10-11 -0.3603415507340557 1106 12-13 -0.5396403104206584 1106 14-15 -0.2023364278665838 1106 16-17 0.07392217781247012 1106 18-19 -0.0049600035428980505 1106 20-21 -0.21072011306215188 1106 22-23 -0.0545095595930718 1106 24-25 -0.10099662492597616 1106 26-27 0.23634622483609036 1106 28-29 -0.0026845925377685376 1106 30-31 0.09543844831326709 1106 32-33 -0.04089147980072738 1106 34-35 0.1239598840248064 1106 36-37 0.11485952524108001 1106 38-39 0.09254308474762496 1106 40-41 -0.06324944434409474 1106 42-43 -0.2548088383493692 1106 44-45 0.08129813261052021 1106 46-47 0.19202014317488647 1106 48-49 -0.21979445919559026 1106 50-51 0.17605280936008683 1106 52-53 0.3295345177473159 1106 54-55 0.42573573789670505 1106 56-57 0.29645121425118504 1106 58-59 0.20795636845236842 1106 60-61 0.5015262687070035 1106 62-63 0.5298321915401658 1106 64-65 0.7804552358860803 1106 66-67 0.7444989066801249 1106 68-69 0.6080321430187361 1106 70-71 0.42510759998505776 1106 72-73 0.6219289604594387 1106 74-75 0.4730319356651478 1106 76-77 0.4160251534215078 1106 78-79 0.6053280921077331 1106 80-81 0.6961724425387423 1106 82-83 0.5994716777104543 1106 84-85 0.6533674261978213 1106 86-87 0.7085797399463694 1106 88-89 0.7081420955712083 1106 90-91 0.7456888322867457 1106 92-93 0.7239562363089931 1106 94-95 0.3177995779125382 1106 96-97 0.9040695643556198 1106 98-99 0.6699992447388432 1106 100-101 0.6524314134809543 1107 1 -0.15594722608350509 1107 2 -0.08682323899565603 1107 3 -0.07802713779101822 1107 4 -0.04773485426650126 1107 5 -0.16362384629881177 1107 6 -0.08927267256672167 1107 7 -0.01437592938180643 1107 8 -0.1986658182084753 1107 9 -0.07544547180489758 1107 10-11 -0.0856421185370948 1107 12-13 -0.014041818431969944 1107 14-15 0.0013922220495174997 1107 16-17 -0.1000554318976512 1107 18-19 -0.21899600271532904 1107 20-21 -0.20405222037003057 1107 22-23 -0.2742576113619961 1107 24-25 -0.22444298248097283 1107 26-27 -0.16712220117710785 1107 28-29 -0.5129597586259038 1107 30-31 -0.2966026541901243 1107 32-33 0.005032521946354507 1107 34-35 -0.1600055420573554 1107 36-37 -0.06764386548557155 1107 38-39 0.0582464019127471 1107 40-41 0.02847534359182191 1107 42-43 0.03697158049157423 1107 44-45 0.14615963004735733 1107 46-47 0.2546458299406993 1107 48-49 0.3556240369309336 1107 50-51 0.44963732465858897 1107 52-53 0.2747854315194118 1107 54-55 0.3581046489562212 1107 56-57 0.3091065555077108 1107 58-59 0.6421100660685148 1107 60-61 0.3164748905777657 1107 62-63 0.4045619678671457 1107 64-65 0.6163422269897332 1107 66-67 0.2641113293830486 1107 68-69 0.4129483518415249 1107 70-71 0.08741973074067388 1107 72-73 0.4851463577606516 1107 74-75 0.39173127591019963 1107 76-77 0.45796028134959954 1107 78-79 0.3204923412054512 1107 80-81 0.3653239562970505 1107 82-83 0.4895270787357191 1107 84-85 0.453263520540478 1107 86-87 0.6281305548701646 1107 88-89 0.7669393645995051 1107 90-91 0.865928826769629 1107 92-93 1.1537109545913324 1107 94-95 1.0310558248953612 1107 96-97 1.2558651552615778 1107 98-99 1.0686273222543043 1107 100-101 1.2575177333356713 1108 1 -0.08641500722344375 1108 2 0.052183656187636984 1108 3 -0.17812634906141867 1108 4 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22.0 5 44.0 6 115.0 7 261.0 8 642.0 9 1150.0 10 1800.0 11 2788.0 12 4015.0 13 5582.0 14 7340.0 15 10284.0 16 13887.0 17 17847.0 18 14595.0 19 8945.0 20 8167.0 21 8257.0 22 8797.0 23 9900.0 24 11132.0 25 13140.0 26 15528.0 27 18593.0 28 22410.0 29 26327.0 30 31242.0 31 37651.0 32 44885.0 33 54912.0 34 68011.0 35 84578.0 36 91530.0 37 76707.0 38 26036.0 39 900.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78680150097221 22.487034991481476 16.971791243972174 17.75437226357414 2 17.62589735705787 24.61425343903319 36.996510835126934 20.763338368782 3 21.136719115543496 27.846343061107177 31.46491450877639 19.552023314572946 4 14.13967354250498 19.718059436118875 36.39573279146558 29.746534229910566 5 12.259260893340125 40.34042063915439 32.68503403270509 14.715284434800397 6 29.014745397911852 39.37689664853014 15.975829846396536 15.632528107161479 7 26.222611392591205 36.89878748178549 19.46967367618946 17.408927449433847 8 27.588733072202988 34.21400211221475 18.56543186770584 19.631832947876422 9 27.692873280483404 16.27474833897036 20.07713594374557 35.95524243680066 10-11 25.319439059930755 28.575257676831146 25.28548320254535 20.819820060692752 12-13 27.168277650770733 26.49112956055562 27.096017326434847 19.2445754622388 14-15 23.195794286325413 26.696923920163627 28.141718915016778 21.965562878494175 16-17 19.84853548654466 30.366830207344623 28.401334171089392 21.383300135021322 18-19 19.9024771733754 26.81563573653463 33.96448003422323 19.31740705586674 20-21 21.436640241701536 25.106212317687792 34.6409234923733 18.816223948237372 22-23 20.813801226035917 25.609859622831195 34.57986779774084 18.996471353392053 24-25 20.57075594348054 25.878930597954213 34.764790772507084 18.78552268605816 26-27 20.138767270709813 26.122002901011342 34.782323883879336 18.95690594439951 28-29 19.657529485057605 26.68982601743836 34.74270259864225 18.909941898861774 30-31 19.93462785334715 26.093847130777714 34.77062932388623 19.200895691988904 32-33 19.894121893506945 26.271874122695614 34.59654024571207 19.23746373808537 34-35 20.42912717404382 26.17669077443418 34.455369437054664 18.938812614467334 36-37 20.607890870541116 25.852339949386987 34.356221483391685 19.183547696680208 38-39 20.036763354288446 26.412665309764684 34.45488300898029 19.095688326966588 40-41 20.113979462039932 26.456643755359917 34.06229299780289 19.367083784797256 42-43 20.08394828336523 26.788981503702402 33.47884012591574 19.64823008701663 44-45 20.501985214496745 26.59291739636111 33.05522505781854 19.84987233132361 46-47 20.915203935671027 27.445022258465567 31.977460797026858 19.662313008836545 48-49 21.03224469606834 27.76426079167948 31.26171410237557 19.941780409876607 50-51 20.844083793430745 28.1820247851022 30.840006416854937 20.133885004612114 52-53 21.28874682666046 28.490539805274928 30.180848603463232 20.039864764601372 54-55 20.698701923719636 28.490969613518175 30.237156263786215 20.57317219897598 56-57 21.222745077069103 28.461224817186476 29.997192625964196 20.31883747978022 58-59 21.598331617715864 28.023073940884725 30.07386067403714 20.304733767362272 60-61 21.048754729088408 28.360493563092387 30.753445717417748 19.837305990401454 62-63 20.944012940657462 28.237236474472947 30.80518161036322 20.01356897450637 64-65 20.738539898132426 28.529069689718327 30.606927529644533 20.125462882504713 66-67 20.95163295589749 28.369517265350318 30.19999197893133 20.47885779982086 68-69 21.007780436613505 28.416507359330506 30.08361964092349 20.492092563132495 70-71 21.14728018929722 28.612020908252344 30.009692124647408 20.231006777803028 72-73 21.255029878480812 28.520914936566715 29.841517051455156 20.38253813349732 74-75 20.890472307260406 29.340668155020523 29.334652353515235 20.434207184203842 76-77 20.92609922061949 28.742630643156026 29.75930109754956 20.57196903867492 78-79 20.795355801237918 28.937141558493646 29.57541809820462 20.69208454206382 80-81 20.89227704771199 29.0712270898226 29.510915337620148 20.52558052484526 82-83 20.97777153914796 28.913308668464726 29.399988636781405 20.70893115560591 84-85 20.65981355013405 28.959419381187505 29.38172892913983 20.999038139538616 86-87 20.727911982139755 28.911540980976696 29.081921848054222 21.278625188829324 88-89 20.67116292127321 28.851115596968036 29.08927449433846 21.38844698742029 90-91 20.832600806303365 28.72183336085184 29.034541629706084 21.411024203138716 92-93 20.816226947320576 28.828165210590694 29.075553297727986 21.28005454436074 94-95 20.51715840273786 28.6146277555713 29.14963303610818 21.71858080558266 96-97 20.518094194083126 28.54758498990682 29.183388366776736 21.75093244923332 98-99 20.5996870419178 28.707654238135564 28.622362800099193 22.07029591984744 100-101 20.429569861923973 28.4021485177968 29.001580972180907 22.16670064809832 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1046.0 1 721.0 2 526.5 3 729.0 4 695.0 5 681.0 6 1265.5 7 2760.5 8 4226.5 9 4684.5 10 4009.0 11 3116.5 12 2943.5 13 3088.5 14 3179.0 15 3149.5 16 3146.5 17 3199.0 18 3274.5 19 3379.5 20 3557.0 21 3859.0 22 4251.5 23 4779.5 24 5304.0 25 6031.5 26 6985.5 27 7853.5 28 8819.0 29 9979.5 30 11124.0 31 12357.0 32 14229.0 33 15774.5 34 16788.0 35 17624.0 36 18941.0 37 20532.0 38 21392.0 39 21577.5 40 21430.0 41 21933.0 42 22573.0 43 23269.0 44 24142.0 45 24644.0 46 24352.0 47 25648.0 48 29304.0 49 28511.5 50 24418.0 51 21493.0 52 19367.0 53 18719.0 54 18403.5 55 17296.5 56 15690.5 57 13943.0 58 12772.0 59 11806.5 60 10075.5 61 8364.0 62 7086.0 63 5570.5 64 4136.5 65 3201.5 66 2329.0 67 1783.0 68 1300.0 69 823.5 70 560.0 71 410.5 72 352.0 73 323.0 74 261.0 75 218.5 76 167.5 77 108.0 78 67.5 79 37.0 80 28.5 81 23.0 82 13.5 83 9.0 84 4.0 85 1.0 86 0.5 87 0.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03315375051802735 2 0.0 3 0.0 4 0.0 5 0.009758966886354825 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005347379115810863 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.00127000254000508 24-25 6.015801505287221E-4 26-27 0.002005267168429074 28-29 0.003208427469486518 30-31 6.684223894763579E-4 32-33 0.0 34-35 0.0 36-37 1.3368447789527158E-4 38-39 3.342111947381789E-4 40-41 6.684223894763579E-5 42-43 0.009291071213721374 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.3368447789527158E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.342111947381789E-4 84-85 6.684223894763579E-5 86-87 0.0 88-89 0.0 90-91 0.006483697177920672 92-93 0.002005267168429074 94-95 0.0 96-97 0.0 98-99 8.689491063192653E-4 100-101 0.00969212464740719 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 748030.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.50822656887252 #Duplication Level Percentage of deduplicated Percentage of total 1 79.48394707800139 44.120129429939745 2 11.49911861364174 12.7659136269673 3 3.7314977501016777 6.213864676616459 4 1.607453598574865 3.569075941945722 5 0.9104126378078632 2.5267695485301873 6 0.5586738014310665 1.8606595168757358 7 0.3829683294483909 1.4880524959806727 8 0.28530373874593584 1.2669363657004657 9 0.22007144026077252 1.099419783059975 >10 1.196178315292166 12.311395634542684 >50 0.08147041418572767 3.070864185451996 >100 0.03808357660920894 3.915092249236813 >500 0.0031334588345065845 1.1403298215327828 >1k 9.641411798481797E-4 1.0960438692944452 >5k 4.8207058992408985E-4 2.108600021982477 >10k+ 2.4103529496204493E-4 1.4468528323425451 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10814 1.4456639439594667 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8833 1.180834993248934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6927 0.9260323783805462 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2919 0.39022499097629776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1998 0.26710158683475266 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1862 0.24892049784099568 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1413 0.18889616726601874 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 917 0.12258866622996403 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 856 0.11443391307835248 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 757 0.10119914976672059 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3368447789527158E-4 0.0 20-21 0.0 0.0 0.0 1.3368447789527158E-4 0.0 22-23 0.0 0.0 0.0 4.0105343368581476E-4 0.0 24-25 0.0 0.0 0.0 6.015801505287221E-4 0.0 26-27 0.0 0.0 0.0 9.357913452669011E-4 0.0 28-29 0.0 0.0 0.0 0.0017378982126385304 0.0 30-31 0.0 0.0 0.0 0.005481063593706134 0.0 32-33 0.0 0.0 0.0 0.009224228974773738 0.0 34-35 0.0 0.0 0.0 0.013568974506370065 0.0 36-37 0.0 0.0 0.0 0.020386882879028914 0.0 38-39 0.0 0.0 0.0 0.03355480395171317 0.0 40-41 0.0 0.0 0.0 0.05213694637915592 0.0 42-43 0.0 0.0 0.0 0.07045171985080811 0.0 44-45 0.0 0.0 0.0 0.09779019558039115 0.0 46-47 0.0 0.0 0.0 0.13121131505420908 0.0 48-49 0.0 0.0 0.0 0.1685092843869898 0.0 50-51 0.0 0.0 0.0 0.21917570150929777 0.0 52-53 0.0 0.0 0.0 0.2703100143042391 0.0 54-55 0.0 0.0 0.0 0.32425170113498125 0.0 56-57 0.0 0.0 0.0 0.40125396040265765 0.0 58-59 0.0 0.0 0.0 0.485608865954574 0.0 60-61 0.0 0.0 0.0 0.5801906340654787 0.0 62-63 0.0 0.0 0.0 0.6840634733901048 0.0 64-65 0.0 0.0 0.0 0.7892731574936834 0.0 66-67 0.0 0.0 0.0 0.9164739382110343 0.0 68-69 0.0 0.0 0.0 1.078365840942208 0.0 70-71 0.0 0.0 0.0 1.2767536061387912 0.0 72-73 0.0 0.0 0.0 1.4783497988048606 0.0 74-75 0.0 0.0 0.0 1.685694424020427 0.0 76-77 0.0 0.0 0.0 1.9228506878066387 0.0 78-79 0.0 0.0 0.0 2.2021844043688086 0.0 80-81 0.0 0.0 0.0 2.5223587289279843 0.0 82-83 0.0 0.0 0.0 2.8640562544282986 0.0 84-85 0.0 0.0 0.0 3.181088993756935 0.0 86-87 0.0 0.0 0.0 3.5451786693047067 0.0 88-89 0.0 0.0 0.0 3.946967367618946 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 280 0.0 30.539066 4 GGACCGA 135 4.2226748E-7 28.147474 6 GGTATCA 7765 0.0 27.35234 1 GATAGTC 175 4.94947E-9 27.145838 7 GTATCAA 10740 0.0 26.854237 1 GGTTGCG 90 8.938882E-4 26.391787 7 GTTGCGG 90 8.938882E-4 26.391787 8 GTATTCG 45 8.950063E-4 26.388258 66-67 CGACCGT 45 8.950063E-4 26.388258 18-19 CGATCCG 45 8.950063E-4 26.388258 70-71 TAAGGTG 325 0.0 26.31058 5 AGTACTT 8045 0.0 23.029253 12-13 GGTAAGG 335 0.0 22.689058 3 GTGCTAA 190 3.2739445E-7 22.506985 1 GTACATG 6320 0.0 22.404104 1 TACATGG 6380 0.0 21.891119 2 TCGACCG 55 0.002878168 21.590391 18-19 GAGTACT 8045 0.0 21.553019 12-13 GTCGGGA 155 3.900772E-5 21.453968 2 TATCAAC 13595 0.0 21.210493 2 >>END_MODULE