FastQCFastQC Report
Thu 26 May 2016
SRR937157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777782
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117891.5157203432324224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79731.0250944352016373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65190.8381525928859244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.24171297355814353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17840.2293701834190043No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16780.21574168597370472No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12510.16084198400065827No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11690.1502991840901435No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11600.1491420475145992No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11180.14374207682872578No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.133199276918211No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.12317075993016038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8910.1145565209788861No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.11327081367272579No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT8160.1049137161826836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48500.040.4820331
GTATCAA79700.037.5782241
CGTCACT1102.7143233E-630.2523671
TATCAAC101700.029.2527962
ATCAACG102200.029.109683
TCAACGC102950.028.8071444
CAACGCA103700.028.6446345
AACGCAG106400.027.94986
GTACATG62000.026.9134541
TACATGG61900.026.1804892
CATTCCG1109.473265E-525.908889
ACGCAGA118750.024.8830037
ACATGGG62800.024.821513
CGCAGAG119500.024.7252438
GAGTACT70250.024.44286212-13
AGTACTT71150.023.86663612-13
GCAGAGT123750.023.4907199
GTACTTT73300.023.1673414-15
GTCGGGA2554.8748916E-1022.3642882
CATGGGA42950.022.3527094
Produced by FastQC (version 0.11.5)