##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937157_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 777782 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3006420822287 33.0 31.0 34.0 31.0 34.0 2 32.40150067756775 34.0 31.0 34.0 31.0 34.0 3 32.433993072609034 34.0 31.0 34.0 31.0 34.0 4 36.02545700466197 37.0 35.0 37.0 35.0 37.0 5 36.04954987387212 37.0 37.0 37.0 35.0 37.0 6 36.1461990634908 37.0 37.0 37.0 35.0 37.0 7 36.116060798527094 37.0 37.0 37.0 35.0 37.0 8 36.117149792615415 37.0 37.0 37.0 35.0 37.0 9 37.82683322576249 39.0 38.0 39.0 35.0 39.0 10-11 37.76669555222415 39.0 38.0 39.0 35.0 39.0 12-13 37.67588411662908 39.0 38.0 39.0 35.0 39.0 14-15 38.91382289638999 40.0 38.0 41.0 36.0 41.0 16-17 38.896333419904295 40.0 38.0 41.0 36.0 41.0 18-19 38.944389301886645 40.0 38.5 41.0 36.0 41.0 20-21 38.92785060595385 40.0 39.0 41.0 36.0 41.0 22-23 38.80618798069382 40.0 38.0 41.0 35.5 41.0 24-25 38.67306520336032 40.0 38.0 41.0 35.0 41.0 26-27 38.499758929880095 40.0 38.0 41.0 35.0 41.0 28-29 38.314593292207846 40.0 38.0 41.0 35.0 41.0 30-31 38.17304398918978 40.0 38.0 41.0 35.0 41.0 32-33 37.90746057378546 40.0 37.0 41.0 34.0 41.0 34-35 37.676494184745856 40.0 37.0 41.0 34.0 41.0 36-37 37.65575379733653 40.0 37.0 41.0 34.0 41.0 38-39 37.60454921816138 40.0 37.0 41.0 34.0 41.0 40-41 37.4003422552849 40.0 36.5 41.0 33.0 41.0 42-43 37.07973185288422 40.0 36.0 41.0 33.0 41.0 44-45 36.70374925107549 40.0 36.0 41.0 32.0 41.0 46-47 36.16075841302576 40.0 36.0 41.0 30.5 41.0 48-49 35.68160358558053 39.5 35.0 41.0 28.0 41.0 50-51 34.68024652151888 38.5 34.5 40.0 25.5 40.5 52-53 34.766623552615 38.5 35.0 40.0 26.0 40.5 54-55 35.08785045166898 39.0 35.0 40.5 26.0 41.0 56-57 34.87567017493333 39.0 35.0 41.0 24.5 41.0 58-59 34.655975581846846 39.0 35.0 40.5 24.0 41.0 60-61 34.3821745681952 38.0 35.0 40.0 22.5 41.0 62-63 34.078466716894965 38.0 34.5 40.0 22.0 41.0 64-65 33.74218611384681 37.0 34.0 40.0 20.0 41.0 66-67 33.357915199888915 37.0 34.0 40.0 18.5 41.0 68-69 32.89473785713734 36.0 33.5 39.0 12.5 41.0 70-71 32.473058003399416 35.5 33.0 39.0 7.5 40.5 72-73 32.08520253747194 35.0 33.0 38.5 4.0 40.0 74-75 31.660079559568103 35.0 32.5 37.5 2.0 39.5 76-77 31.24770244104389 35.0 32.0 37.0 2.0 39.0 78-79 30.80793525692289 35.0 32.0 36.5 2.0 39.0 80-81 30.39040245210097 35.0 31.0 36.0 2.0 38.5 82-83 29.98586686243703 35.0 31.0 35.5 2.0 37.0 84-85 29.547632884278627 34.0 30.5 35.0 2.0 37.0 86-87 29.180107665129817 34.0 30.0 35.0 2.0 36.0 88-89 28.840578079718995 34.0 29.5 35.0 2.0 36.0 90-91 28.455183457575515 34.0 29.0 35.0 2.0 36.0 92-93 28.083191048391452 34.0 29.0 35.0 2.0 35.0 94-95 27.638183964144194 34.0 28.0 35.0 2.0 35.0 96-97 27.132463209485433 34.0 27.0 35.0 2.0 35.0 98-99 26.579284426741687 33.5 25.5 35.0 2.0 35.0 100-101 24.92917295591824 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13348624650069496 1101 2 0.028752302450612888 1101 3 0.20457010990422475 1101 4 0.11368078423020478 1101 5 0.1628175651520536 1101 6 -0.0031552836893951053 1101 7 0.10670033466072226 1101 8 0.10293087009319635 1101 9 0.049711704225885 1101 10-11 0.12696606599907767 1101 12-13 0.1563024755058109 1101 14-15 0.2128488913659936 1101 16-17 0.09159504927595918 1101 18-19 0.13323970010167585 1101 20-21 0.16902411161972708 1101 22-23 0.16137154162893097 1101 24-25 0.20401215786981197 1101 26-27 0.2614012773420882 1101 28-29 0.13605214985073388 1101 30-31 0.2253882425904976 1101 32-33 0.450456940305358 1101 34-35 0.33828570733462016 1101 36-37 0.39087612670104477 1101 38-39 0.546317209718211 1101 40-41 0.45440699571949494 1101 42-43 0.4449327371427927 1101 44-45 0.7889687449112159 1101 46-47 0.848251542911548 1101 48-49 1.1108288628183516 1101 50-51 0.991960183112127 1101 52-53 1.0083718570858906 1101 54-55 1.148679000354285 1101 56-57 1.1489140953027146 1101 58-59 1.0610963110790195 1101 60-61 1.1644988553457551 1101 62-63 1.1210022122433898 1101 64-65 1.0885764233859376 1101 66-67 1.0984181574100518 1101 68-69 1.1752715962887095 1101 70-71 1.1125895951622269 1101 72-73 1.278469031641965 1101 74-75 1.1869020898803022 1101 76-77 1.0423608962003392 1101 78-79 1.339766905732116 1101 80-81 1.3089097481271246 1101 82-83 1.2287138163908935 1101 84-85 1.0029408314624924 1101 86-87 1.189310329660529 1101 88-89 1.3624215961987112 1101 90-91 1.3811387728139017 1101 92-93 1.1928543539343188 1101 94-95 1.1757268543043615 1101 96-97 1.1589545913357142 1101 98-99 1.170387918484792 1101 100-101 1.3017561547110823 1102 1 -0.09673931136779146 1102 2 -0.14858864718684828 1102 3 -0.01388058027994532 1102 4 -0.0426615149750873 1102 5 -0.05469051067967001 1102 6 -0.02845782049004697 1102 7 -0.03695275349864602 1102 8 -0.0816340616770006 1102 9 0.06340602339346901 1102 10-11 -0.04531623621185332 1102 12-13 0.07743719868073384 1102 14-15 0.01013398347734551 1102 16-17 0.03728126806068843 1102 18-19 0.047322670995946226 1102 20-21 0.06184878535017191 1102 22-23 0.04142553922143577 1102 24-25 0.12365930614521403 1102 26-27 0.11602142682123429 1102 28-29 0.0921566007462502 1102 30-31 -0.12595358791151767 1102 32-33 -0.042881893471630406 1102 34-35 0.04106422495265605 1102 36-37 0.10247147359353903 1102 38-39 0.28106575413417545 1102 40-41 0.18129806461589482 1102 42-43 0.24268764848348212 1102 44-45 0.19744550392482552 1102 46-47 0.37878198109702765 1102 48-49 0.43559484597182063 1102 50-51 0.33657463375470087 1102 52-53 0.40442760747981765 1102 54-55 0.4566933054756461 1102 56-57 0.572835371008857 1102 58-59 0.36674887250517685 1102 60-61 0.551009426464411 1102 62-63 0.7147272426347016 1102 64-65 0.688262169003572 1102 66-67 0.644182939790376 1102 68-69 0.7604316324160578 1102 70-71 0.7120499168265866 1102 72-73 0.5093425903794149 1102 74-75 0.5517905608214484 1102 76-77 0.37800897890638296 1102 78-79 0.5173611783269614 1102 80-81 0.4610742644625496 1102 82-83 0.7005949681213011 1102 84-85 0.7306100965804028 1102 86-87 0.7700846423406595 1102 88-89 0.7579201104837487 1102 90-91 0.6453375059759203 1102 92-93 0.2515121960839828 1102 94-95 0.473972044144066 1102 96-97 0.2974422718794685 1102 98-99 0.45973146607751403 1102 100-101 0.23833241868476662 1103 1 -0.18849801994893056 1103 2 -0.2072122665581304 1103 3 -0.1600148198721243 1103 4 -0.2040888726641228 1103 5 -0.15834386666947609 1103 6 -0.20942638463618124 1103 7 -0.1057717849344968 1103 8 -0.07653635564471273 1103 9 -0.04109695026838267 1103 10-11 -0.0576356924565502 1103 12-13 -0.05722720233880807 1103 14-15 -0.1453460505073636 1103 16-17 0.03813088573273404 1103 18-19 -0.06949975891059523 1103 20-21 -0.1641495154144863 1103 22-23 -0.20878686519657919 1103 24-25 -0.2582438374401761 1103 26-27 -0.24506608379898864 1103 28-29 -0.39127585464881776 1103 30-31 -0.37021866862519914 1103 32-33 -0.3534171526050187 1103 34-35 -0.17898675210766868 1103 36-37 -0.01180210329685849 1103 38-39 -0.07619847696183513 1103 40-41 0.054280222644784715 1103 42-43 -3.0300572213093346E-4 1103 44-45 0.1320249431771643 1103 46-47 0.15490772451249057 1103 48-49 0.0600638349267939 1103 50-51 0.027738609965403782 1103 52-53 -0.014009096003618993 1103 54-55 0.01744096902704939 1103 56-57 0.09365100397402415 1103 58-59 -0.10776259733339089 1103 60-61 0.025520896302978713 1103 62-63 0.013367854359422893 1103 64-65 0.07483820978522004 1103 66-67 0.06771734930609341 1103 68-69 0.19755992468454053 1103 70-71 0.07653589813499906 1103 72-73 0.051823473981791324 1103 74-75 0.0522153696574712 1103 76-77 -0.05189756314969429 1103 78-79 0.049221841028749935 1103 80-81 0.0723741795007804 1103 82-83 0.022600065827333538 1103 84-85 0.052190385450412435 1103 86-87 0.22250605270599522 1103 88-89 0.09097023792639902 1103 90-91 0.2596110828663214 1103 92-93 0.21540344502196263 1103 94-95 0.08484715034627399 1103 96-97 -0.1936367425640313 1103 98-99 -0.07977575567269213 1103 100-101 0.22601296244007685 1104 1 -0.056531266634152644 1104 2 0.012990481084592886 1104 3 0.04082229904607715 1104 4 0.03662299088519205 1104 5 0.04197693467919805 1104 6 -0.01191185111496651 1104 7 -0.008886355356793274 1104 8 0.06965591387603354 1104 9 0.07773271998770781 1104 10-11 -0.020144266750484974 1104 12-13 0.10026044398216527 1104 14-15 0.19489792814357543 1104 16-17 0.12749697572078844 1104 18-19 0.14506106612619618 1104 20-21 0.12611693123443501 1104 22-23 0.08956768873927246 1104 24-25 0.19043947836018305 1104 26-27 0.2154292983578472 1104 28-29 0.1181011866283157 1104 30-31 0.17591363663603232 1104 32-33 0.16630632734563733 1104 34-35 0.24896871959381173 1104 36-37 0.22275003213011502 1104 38-39 0.32433822548003377 1104 40-41 0.2718325648963713 1104 42-43 0.14414464607449418 1104 44-45 -0.07924491533396605 1104 46-47 0.06672790017950092 1104 48-49 -0.017892678337510404 1104 50-51 -0.11486993947981006 1104 52-53 0.023695850080628134 1104 54-55 0.19421315096725067 1104 56-57 0.12001742279834104 1104 58-59 0.06109631107901947 1104 60-61 0.006442813314230023 1104 62-63 0.054014471437788814 1104 64-65 0.12929446191483862 1104 66-67 0.06251623096521541 1104 68-69 0.24094585198048435 1104 70-71 0.23693285260530672 1104 72-73 0.19615729784161928 1104 74-75 0.1952208289345947 1104 76-77 0.12511045837197088 1104 78-79 0.02059002307012392 1104 80-81 0.1744964381446401 1104 82-83 0.19456320343117284 1104 84-85 0.33787953549051153 1104 86-87 0.38633309673583227 1104 88-89 0.3304601250953887 1104 90-91 0.489720208890958 1104 92-93 0.5085286096260866 1104 94-95 0.5128547001887789 1104 96-97 0.49652026559140694 1104 98-99 0.6239781111292757 1104 100-101 0.6248734927145883 1105 1 -0.024698914944835337 1105 2 0.026467687933049433 1105 3 -0.08751819263392235 1105 4 0.11904660013872359 1105 5 0.0786047898164739 1105 6 0.0638926006868985 1105 7 0.08117674912814721 1105 8 0.056698549358891626 1105 9 0.13638826303788676 1105 10-11 0.12288765897094578 1105 12-13 0.12333328415504496 1105 14-15 0.1037543423081857 1105 16-17 0.12128242047197801 1105 18-19 0.13486063241955293 1105 20-21 0.12597982071662983 1105 22-23 0.12509655681203213 1105 24-25 0.04200051945800709 1105 26-27 0.0441601764876296 1105 28-29 0.028769899017554224 1105 30-31 0.1477832689274905 1105 32-33 0.11646875704495585 1105 34-35 0.12183993160032003 1105 36-37 0.29916979892447415 1105 38-39 0.3291303642527055 1105 40-41 0.24818688875646444 1105 42-43 0.4448995109743663 1105 44-45 0.42281006009549316 1105 46-47 0.3960526434968372 1105 48-49 0.43523655379311776 1105 50-51 0.32391484074801014 1105 52-53 0.2081544990625659 1105 54-55 0.31401956437480294 1105 56-57 0.4925811876008055 1105 58-59 0.49773526964472126 1105 60-61 0.4552572699780555 1105 62-63 0.34085261878028206 1105 64-65 0.3554944312962931 1105 66-67 0.4828457672624893 1105 68-69 0.4208714421865807 1105 70-71 0.28757121562348686 1105 72-73 0.23632395020841201 1105 74-75 0.4011233946677315 1105 76-77 0.4803919891646373 1105 78-79 0.5176762142745233 1105 80-81 0.3567247279979533 1105 82-83 0.5691470351570338 1105 84-85 0.5061482081765796 1105 86-87 0.45951184006326073 1105 88-89 0.4086909283611426 1105 90-91 0.5308807022196476 1105 92-93 0.44052641922247915 1105 94-95 0.5376729656650205 1105 96-97 0.29920435118201283 1105 98-99 0.4748535996380667 1105 100-101 0.6353536150541785 1106 1 -0.05512140420588452 1106 2 -0.07213730605555924 1106 3 -0.08638841297116073 1106 4 0.009451931248747769 1106 5 0.03940687296100265 1106 6 -0.01711622609431629 1106 7 0.06584920364998936 1106 8 0.019965606313078865 1106 9 -0.0713492172513881 1106 10-11 -0.1618198368452468 1106 12-13 -0.2076090849989498 1106 14-15 -0.15389432642470524 1106 16-17 -0.28918254125446907 1106 18-19 -0.17260406951799467 1106 20-21 -0.10296368425862568 1106 22-23 -0.04176797233568408 1106 24-25 -0.19602804523849215 1106 26-27 -0.16390355033527726 1106 28-29 0.13581350126261071 1106 30-31 0.05517305499434855 1106 32-33 0.1261096441938605 1106 34-35 -0.026464794745294284 1106 36-37 -0.20955729585014637 1106 38-39 -0.11810983173688072 1106 40-41 -0.010404269962471346 1106 42-43 0.04830153732783771 1106 44-45 0.05885184381820352 1106 46-47 0.23032242521373547 1106 48-49 0.27069955199876006 1106 50-51 0.0787254667184456 1106 52-53 0.11687343767384561 1106 54-55 0.1285030979872559 1106 56-57 0.13811432881117724 1106 58-59 0.11338513461490862 1106 60-61 0.09419298128612041 1106 62-63 0.14569224444259277 1106 64-65 0.037166928109350295 1106 66-67 0.1556763788539115 1106 68-69 -0.041570936029813765 1106 70-71 0.0507842002355261 1106 72-73 0.23805940051925134 1106 74-75 0.16593589353493243 1106 76-77 0.2732873667345821 1106 78-79 0.4838115220035455 1106 80-81 0.19128903783549944 1106 82-83 0.10727105717263186 1106 84-85 0.23959119659483008 1106 86-87 0.20743201841136738 1106 88-89 0.36340005541000053 1106 90-91 0.20445896211730386 1106 92-93 0.26357694514397423 1106 94-95 0.42592144469159976 1106 96-97 0.552560461356677 1106 98-99 0.5192563570254372 1106 100-101 0.9853934896305141 1107 1 -0.0340449963370304 1107 2 -0.12977568444865284 1107 3 -0.15300313996324633 1107 4 -0.10892235693685848 1107 5 0.06511491753697385 1107 6 -0.02986762563816825 1107 7 -0.11455739185094416 1107 8 0.04712502969891119 1107 9 0.0391032233897306 1107 10-11 -0.04286541600402671 1107 12-13 0.016563492947213376 1107 14-15 0.16536660132678094 1107 16-17 0.20166361468603355 1107 18-19 0.21420252011878205 1107 20-21 0.18165421356681577 1107 22-23 0.13854804288599354 1107 24-25 0.024837567136557936 1107 26-27 0.1846724020142645 1107 28-29 0.12464280612964984 1107 30-31 0.08548917493310881 1107 32-33 0.11871818868920059 1107 34-35 0.1317632455582114 1107 36-37 0.19283904994242818 1107 38-39 0.20351246630050923 1107 40-41 0.06834559652324401 1107 42-43 0.21924380260972498 1107 44-45 0.33664742350057963 1107 46-47 0.2394820287129633 1107 48-49 0.35572144834141284 1107 50-51 0.3491835201953606 1107 52-53 0.29359451533831304 1107 54-55 0.4208459752357996 1107 56-57 0.43964613034298594 1107 58-59 0.43575930132869445 1107 60-61 0.2376218794319982 1107 62-63 0.36404988261154614 1107 64-65 0.45355295298593745 1107 66-67 0.44291691609444683 1107 68-69 0.43998105495613515 1107 70-71 0.332023401286051 1107 72-73 0.32563377582947695 1107 74-75 0.4369007777073648 1107 76-77 0.3484077390247684 1107 78-79 0.4120085869930641 1107 80-81 0.2500609786829102 1107 82-83 0.3000002357508684 1107 84-85 0.41098112741872583 1107 86-87 0.2860546536377768 1107 88-89 0.29843566969089963 1107 90-91 0.40832218484844773 1107 92-93 0.6357634588399215 1107 94-95 0.7010907198608756 1107 96-97 0.6389745713556962 1107 98-99 0.9548438303487607 1107 100-101 1.0415338901105322 1108 1 -0.03489152344717894 1108 2 -0.05828919274411959 1108 3 -0.10539762357676352 1108 4 -0.02481617570481376 1108 5 0.02785881387848832 1108 6 0.07629776496726492 1108 7 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-1.3440681710924096 2316 96-97 -1.411138866608212 2316 98-99 -1.2199626715605447 2316 100-101 -1.9361224099756704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3024.0 3 411.0 4 319.0 5 346.0 6 361.0 7 424.0 8 516.0 9 611.0 10 860.0 11 1185.0 12 2064.0 13 3105.0 14 3761.0 15 5486.0 16 8351.0 17 13370.0 18 11000.0 19 5522.0 20 4448.0 21 4251.0 22 4084.0 23 4396.0 24 4576.0 25 5065.0 26 5549.0 27 6504.0 28 7466.0 29 9137.0 30 11514.0 31 14974.0 32 20120.0 33 28981.0 34 44374.0 35 74532.0 36 114242.0 37 172307.0 38 155431.0 39 25104.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.772841292625706 19.159692693804224 15.285091367749873 20.782374645820195 2 14.845447219983884 20.909073327961323 41.05063658340048 23.194842868654312 3 18.773844596728964 27.037306841844007 30.46925263829705 23.719595923129987 4 11.413257899066268 16.68583467208528 38.13302767670881 33.76787975213964 5 12.15001102225981 38.93474693278227 33.26092589560695 15.654316149350974 6 29.21088468679019 37.18887412986538 17.834168413485138 15.766072769859296 7 26.67212983793451 35.554679386578634 20.163096278085728 17.61009449740113 8 27.22639032227724 32.6561298128027 19.7128584920368 20.404621372883256 9 27.81803376692985 15.842549836112887 20.681084953307632 35.65833144364963 10-11 24.497842674014738 27.474095659384094 26.259717953062616 21.76834371353855 12-13 26.356260018451994 25.45917368065685 27.779266765283456 20.405299535607703 14-15 23.042143451609494 25.53072251438407 28.45475299082975 22.972381043176682 16-17 20.012729932800642 29.530545074334174 28.389618404802846 22.067106588062337 18-19 19.860993912847434 26.450706947083642 33.562369158427 20.12592998164192 20-21 21.349846908439456 25.068493415420214 33.61263229230213 19.969027383838196 22-23 20.487407061939813 25.293710681290253 33.997969566690585 20.22091269007935 24-25 20.167240689652065 25.538859112408236 34.062762279765515 20.231137918174188 26-27 19.76039764945675 25.841223663647106 34.13022555887245 20.268153128023698 28-29 19.496135791326935 26.290469187147263 34.043945750980946 20.169449270544852 30-31 19.603066870849844 25.934270110636444 34.022075749210316 20.440587269303396 32-33 19.464183783519115 26.118914503142044 33.85644908367232 20.56045262966652 34-35 20.148678768826418 26.155932334411276 33.5673885875517 20.128000309210613 36-37 20.016825679646015 25.88462041408436 33.62204962802494 20.476504278244693 38-39 19.80985813432956 26.232943108806623 33.54606206019227 20.41113669667155 40-41 19.76300420542899 26.45330131650732 33.59654032773876 20.187154150324922 42-43 19.637325823836427 26.555033323177824 32.88782195175208 20.91981890123367 44-45 19.63495293736427 26.685619274196306 32.82775867700234 20.85166911143708 46-47 20.18557703765971 27.096115507373803 31.980379463494916 20.737927991471572 48-49 20.211079380560676 27.323525565497032 31.351465914925335 21.11392913901696 50-51 20.167710454651754 27.7363276229401 31.214812018304354 20.88114990410379 52-53 20.420762098166655 27.92113577676183 30.63307596756985 21.02502615750166 54-55 19.90933784152935 27.864117577325697 30.84684589494735 21.3796986861976 56-57 20.383098083543462 28.058714225446913 30.382035353486557 21.176152337523067 58-59 20.679957108218026 27.540645759606374 30.626020370376335 21.153376761799265 60-61 20.395758888891752 27.7878826132994 31.143969001914197 20.67238949589466 62-63 20.236246590429694 27.883796047313723 31.12155943360073 20.758397928655846 64-65 20.103024725032313 27.99624123346281 31.014332390636064 20.886401650868812 66-67 20.085526239512117 28.054641978839374 30.61482532112597 21.245006460522536 68-69 20.15024819161797 28.01799010840309 30.751736890478163 21.080024809500777 70-71 20.311058839290773 28.103954222939027 30.51164264624802 21.073344291522176 72-73 20.22376007889836 28.10792739134888 30.573694405418273 21.094618124334488 74-75 19.951408878442972 28.778516850053492 30.16713825732812 21.102936014175416 76-77 19.89276125594554 28.355655413727177 30.34969827830541 21.40188505202187 78-79 19.978344131208026 28.391861721931434 30.378893966115843 21.2509001807447 80-81 20.13608000948077 28.656989236230977 29.99614843593007 21.210782318358177 82-83 19.982375540135585 28.522186463693355 30.12075589350085 21.37468210267021 84-85 20.020777487662965 28.397080589086215 29.94534477361813 21.63679714963269 86-87 19.95491477281876 28.49489409802172 29.942387520409113 21.607803608750398 88-89 19.978164532501996 28.428426042820703 29.624867956612476 21.968541468064824 90-91 19.897859612036566 28.42971874357919 29.7521076357959 21.920314008588345 92-93 20.06300951261994 28.45631203042389 29.864911521297756 21.615766935658414 94-95 19.965693334741843 28.172432539672315 29.88390098861941 21.97797313696643 96-97 19.947069056910745 28.04882683053918 29.94205163117863 22.06205248137144 98-99 19.96354290793618 28.312603461491392 29.443932728291607 22.27992090228082 100-101 19.96551817412485 27.866519138894652 30.031970075391158 22.135992611589344 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1657.0 1 1066.0 2 727.0 3 986.5 4 778.0 5 701.5 6 1473.0 7 3444.5 8 5574.0 9 5901.0 10 4373.5 11 3051.0 12 2838.0 13 2890.0 14 2869.5 15 2784.0 16 2779.5 17 2878.0 18 2884.0 19 2950.0 20 3174.0 21 3466.0 22 3838.5 23 4397.5 24 5050.0 25 5823.0 26 6728.0 27 7715.0 28 8662.5 29 9868.0 30 11267.0 31 12536.0 32 14282.0 33 15826.0 34 17301.0 35 18068.0 36 19212.0 37 21185.5 38 21898.0 39 22010.5 40 21963.5 41 22631.5 42 23265.0 43 24070.0 44 25454.0 45 26109.5 46 25698.5 47 26991.5 48 31131.5 49 30278.0 50 25543.5 51 22548.0 52 20625.5 53 20206.5 54 20026.5 55 18509.0 56 16484.0 57 15036.5 58 13870.5 59 12469.5 60 10685.0 61 9128.5 62 7782.5 63 5958.5 64 4310.0 65 3359.0 66 2434.5 67 1768.5 68 1341.5 69 905.5 70 652.5 71 521.0 72 450.0 73 393.0 74 307.5 75 236.0 76 172.5 77 116.0 78 80.0 79 60.5 80 41.5 81 22.0 82 14.0 83 11.0 84 7.5 85 2.0 86 2.0 87 3.5 88 2.5 89 4.5 90 4.5 91 2.0 92 1.0 93 0.0 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3084411827478651 2 0.2773270659387849 3 0.28118418785726595 4 0.2807984756654178 5 0.2672985489507343 6 0.20687030556119837 7 0.21548454451247265 8 0.21201313478583975 9 0.21059885674906334 10-11 0.21927738106564562 12-13 0.22037023227588193 14-15 0.2026274714508693 16-17 0.21323455672669203 18-19 0.20018462756916464 20-21 0.2073203031183545 22-23 0.20481317387134185 24-25 0.1974203568609199 26-27 0.20841315432859078 28-29 0.20834886896328275 30-31 0.21619168353086082 32-33 0.2179273883941773 34-35 0.2072560177530465 36-37 0.20410603485295367 38-39 0.19536322517106336 40-41 0.19587750809352747 42-43 0.1954917959016794 44-45 0.21027742992252327 46-47 0.19986320074262456 48-49 0.20937743480821105 50-51 0.21632025426147686 52-53 0.22037023227588193 54-55 0.22037023227588193 56-57 0.190220395946422 58-59 0.18122044480329963 60-61 0.1897703983892659 62-63 0.18957754229334184 64-65 0.18816326425656546 66-67 0.1965846471119157 68-69 0.18854897644841356 70-71 0.19259895446281863 72-73 0.20487745923664985 74-75 0.19394894713428698 76-77 0.20185604706717308 78-79 0.19915606172423633 80-81 0.18951325692803384 82-83 0.20417032021826167 84-85 0.18784183743002536 86-87 0.18957754229334184 88-89 0.19568465199760343 90-91 0.19253466909751063 92-93 0.1992846324548524 94-95 0.19902749099362035 96-97 0.19124896179135029 98-99 0.1963275056506836 100-101 0.18398471551154436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 777782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.818536674811924 #Duplication Level Percentage of deduplicated Percentage of total 1 77.85038345139964 41.89793716927322 2 12.422562080694398 13.371282258699587 3 3.953317782281622 6.382853341587288 4 1.8080898596566177 3.892350016931409 5 0.9861505166023835 2.653658887232505 6 0.5980567890669195 1.9311924741610962 7 0.42669627656734094 1.6074918446611592 8 0.29055273503753254 1.2509698421267486 9 0.22702296804930974 1.099623953878779 >10 1.3046405972605943 13.017580562058036 >50 0.08540118576930329 3.140966481431285 >100 0.04305906787151902 4.33362190669742 >500 0.0014353022622130064 0.611014811437358 >1k 0.0019137363496173417 1.425958712911963 >5k 4.784340874043354E-4 1.865745184860976 >10k+ 2.392170437021677E-4 1.5177525520512039 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11789 1.5157203432324224 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7973 1.0250944352016373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6519 0.8381525928859244 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1880 0.24171297355814353 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1784 0.2293701834190043 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1678 0.21574168597370472 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1251 0.16084198400065827 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1169 0.1502991840901435 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1160 0.1491420475145992 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1118 0.14374207682872578 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.133199276918211 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 958 0.12317075993016038 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 891 0.1145565209788861 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 881 0.11327081367272579 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 816 0.1049137161826836 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.285707306160338E-4 0.0 22-23 0.0 0.0 0.0 1.928560959240507E-4 0.0 24-25 0.0 0.0 0.0 7.714243836962028E-4 0.0 26-27 0.0 0.0 0.0 0.0014142780367763718 0.0 28-29 0.0 0.0 0.0 0.002507129247012659 0.0 30-31 0.0 0.0 0.0 0.0052071145899493685 0.0 32-33 0.0 0.0 0.0 0.009514234065586501 0.0 34-35 0.0 0.0 0.0 0.01427135109837975 0.0 36-37 0.0 0.0 0.0 0.0232070168761941 0.0 38-39 0.0 0.0 0.0 0.03837836308888609 0.0 40-41 0.0 0.0 0.0 0.05952824827522365 0.0 42-43 0.0 0.0 0.0 0.08074241882686922 0.0 44-45 0.0 0.0 0.0 0.106520850315384 0.0 46-47 0.0 0.0 0.0 0.14284208171441354 0.0 48-49 0.0 0.0 0.0 0.18437042770339246 0.0 50-51 0.0 0.0 0.0 0.23406301508648952 0.0 52-53 0.0 0.0 0.0 0.2846555975838988 0.0 54-55 0.0 0.0 0.0 0.33904101663448116 0.0 56-57 0.0 0.0 0.0 0.4179834452327259 0.0 58-59 0.0 0.0 0.0 0.5018758469596879 0.0 60-61 0.0 0.0 0.0 0.6131538143078652 0.0 62-63 0.0 0.0 0.0 0.7295746108806838 0.0 64-65 0.0 0.0 0.0 0.8449025562432662 0.0 66-67 0.0 0.0 0.0 0.9847875111535109 0.0 68-69 0.0 0.0 0.0 1.14948661707265 0.0 70-71 0.0 0.0 0.0 1.353849793386836 0.0 72-73 0.0 0.0 0.0 1.5571201184907855 0.0 74-75 0.0 0.0 0.0 1.7740832264053426 0.0 76-77 0.0 0.0 0.0 2.012710502428701 0.0 78-79 0.0 0.0 0.0 2.3005160829126927 0.0 80-81 0.0 0.0 0.0 2.6274714508692667 0.0 82-83 0.0 0.0 0.0 2.9656767577547436 0.0 84-85 0.0 0.0 0.0 3.2848535965090475 0.0 86-87 0.0 0.0 0.0 3.626658883851773 0.0 88-89 0.0 0.0 0.0 4.0288924145840355 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4850 0.0 40.482033 1 GTATCAA 7970 0.0 37.578224 1 CGTCACT 110 2.7143233E-6 30.252367 1 TATCAAC 10170 0.0 29.252796 2 ATCAACG 10220 0.0 29.10968 3 TCAACGC 10295 0.0 28.807144 4 CAACGCA 10370 0.0 28.644634 5 AACGCAG 10640 0.0 27.9498 6 GTACATG 6200 0.0 26.913454 1 TACATGG 6190 0.0 26.180489 2 CATTCCG 110 9.473265E-5 25.90888 9 ACGCAGA 11875 0.0 24.883003 7 ACATGGG 6280 0.0 24.82151 3 CGCAGAG 11950 0.0 24.725243 8 GAGTACT 7025 0.0 24.442862 12-13 AGTACTT 7115 0.0 23.866636 12-13 GCAGAGT 12375 0.0 23.490719 9 GTACTTT 7330 0.0 23.16734 14-15 GTCGGGA 255 4.8748916E-10 22.364288 2 CATGGGA 4295 0.0 22.352709 4 >>END_MODULE