FastQCFastQC Report
Thu 26 May 2016
SRR937157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777782
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111891.4385779048628022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91971.1824650094756628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76090.9782946892574012No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32620.41939772326950225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.2842698853920507No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.26858425625689464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15260.19619893492006757No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.14014209637147684No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.12252790627708021No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9200.11828507216675109No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.11609936974627852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT953.4900044E-529.9992496
GGTATCA83400.028.3274971
GTATCAA119250.028.2621841
TAAGGTG2850.026.6728575
GTAAGGT2451.0913936E-1125.2099364
GAGTACT85250.023.76333412-13
TACGCAT1401.7753287E-523.7555145
AGTACTT85900.023.38998412-13
AGGACCG1852.5337613E-723.1134725
CGCATTC1252.2553741E-422.799437
GTACTTT88550.022.79728314-15
TATCAAC148400.022.6990012
ATCAACG149900.022.408473
TAGACTA1703.1404816E-622.3581315
TCAACGC151250.022.208464
CAACGCA152000.022.1301365
CTCTACG1302.9357118E-421.928172
AACGCAG156000.021.5875996
TACTTTT94150.021.23951114-15
AGGTAAG2509.451469E-920.9048522
Produced by FastQC (version 0.11.5)