##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937157_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 777782 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23396530133122 31.0 31.0 33.0 30.0 34.0 2 31.462843058851966 31.0 31.0 34.0 30.0 34.0 3 31.44706357308346 31.0 31.0 34.0 28.0 34.0 4 35.29402840384581 37.0 35.0 37.0 33.0 37.0 5 35.124822893818575 35.0 35.0 37.0 32.0 37.0 6 35.30595076769583 37.0 35.0 37.0 33.0 37.0 7 35.23133602989012 37.0 35.0 37.0 32.0 37.0 8 35.23078317574848 37.0 35.0 37.0 32.0 37.0 9 36.71098071181899 39.0 35.0 39.0 32.0 39.0 10-11 36.59262814001867 38.0 35.0 39.0 32.0 39.0 12-13 36.45992771753525 38.0 35.0 39.0 32.0 39.0 14-15 37.21365176874754 39.0 36.0 40.0 32.0 41.0 16-17 37.20585009681376 39.0 36.0 40.0 32.0 41.0 18-19 37.330543777048064 39.0 36.0 40.0 32.0 41.0 20-21 37.260232944449726 39.0 36.0 40.0 32.0 41.0 22-23 37.08364554592418 39.0 36.0 40.0 31.0 41.0 24-25 36.86214517692618 38.5 35.5 40.0 31.0 41.0 26-27 36.64937026056144 38.0 35.0 40.0 30.5 41.0 28-29 36.46925166691952 38.0 35.0 40.0 30.0 41.0 30-31 36.35733341733288 38.0 35.0 40.0 30.0 41.0 32-33 36.087333597331906 38.0 35.0 40.0 30.0 41.0 34-35 35.81022188736689 38.0 35.0 40.0 30.0 41.0 36-37 35.477970691016246 38.0 34.0 40.0 29.0 41.0 38-39 35.21846995687738 38.0 34.0 40.0 27.5 41.0 40-41 34.888900745967376 38.0 34.0 40.0 26.5 41.0 42-43 34.471313298584946 37.5 33.0 40.0 25.0 41.0 44-45 33.826300300084085 37.0 33.0 40.0 23.5 41.0 46-47 33.06288008722238 37.0 32.5 40.0 19.0 41.0 48-49 32.44832228053619 37.0 31.0 40.0 11.5 41.0 50-51 32.03147797197672 36.0 31.0 39.0 8.5 40.0 52-53 31.677500250712924 36.0 30.5 39.0 7.0 40.0 54-55 31.31336929885238 35.5 30.0 39.0 3.5 40.0 56-57 30.958276869354137 35.0 29.5 39.0 2.0 40.0 58-59 30.607379059942247 35.0 29.0 38.5 2.0 40.0 60-61 30.11283560174959 35.0 28.0 38.0 2.0 40.0 62-63 29.62560846098264 34.0 27.5 38.0 2.0 40.0 64-65 29.155118657927286 34.0 26.5 37.5 2.0 40.0 66-67 28.596962259347734 34.0 26.0 37.0 2.0 39.0 68-69 28.075600875309533 33.0 26.0 36.0 2.0 39.0 70-71 27.576398013839352 33.0 25.0 36.0 2.0 39.0 72-73 27.016318197129785 32.0 23.5 35.5 2.0 38.0 74-75 26.457668086944672 32.0 22.0 35.0 2.0 37.5 76-77 25.56871076985582 30.5 21.5 34.0 2.0 36.0 78-79 26.17028228989614 32.0 23.0 35.0 2.0 36.0 80-81 25.946962645059926 32.0 22.0 35.0 2.0 36.0 82-83 25.48723806927905 31.5 20.5 34.5 2.0 36.0 84-85 24.941958672224352 31.0 19.0 34.0 2.0 35.5 86-87 24.357743557963545 31.0 16.5 34.0 2.0 35.0 88-89 23.69373487686781 30.5 8.5 34.0 2.0 35.0 90-91 22.907625144320647 29.5 3.5 34.0 2.0 35.0 92-93 22.179390883306635 29.0 2.0 34.0 2.0 35.0 94-95 21.3728975471276 29.0 2.0 34.0 2.0 35.0 96-97 20.445879436654486 28.0 2.0 33.0 2.0 35.0 98-99 19.463897981696668 27.0 2.0 33.0 2.0 34.0 100-101 17.48235816719852 22.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2322229642951541 1101 2 0.24249015591605527 1101 3 0.17722797630585063 1101 4 0.13889944558295753 1101 5 0.00561042523238342 1101 6 0.21216891298689688 1101 7 0.08509292098693066 1101 8 0.038540781894340626 1101 9 0.3224809403998776 1101 10-11 0.18392066623885484 1101 12-13 0.2099694540403121 1101 14-15 0.13280394207667712 1101 16-17 0.09748108378751397 1101 18-19 0.11532059687496599 1101 20-21 0.11373646847255969 1101 22-23 0.19232265894722644 1101 24-25 0.18513271137707932 1101 26-27 0.16506181025916788 1101 28-29 0.2965876028132399 1101 30-31 0.296143756711551 1101 32-33 -0.037314243282636994 1101 34-35 -0.34135335815075507 1101 36-37 0.005629193508660535 1101 38-39 0.07906877300079174 1101 40-41 -0.021808227483333553 1101 42-43 0.26919107144923515 1101 44-45 0.24678632432863878 1101 46-47 0.378634074202985 1101 48-49 0.014998791289585256 1101 50-51 -0.3428519422964449 1101 52-53 -0.35630538308653215 1101 54-55 -0.33502413591952873 1101 56-57 -0.2078166492068938 1101 58-59 -0.35041279876783804 1101 60-61 -0.47257890631140853 1101 62-63 -0.2978665568689678 1101 64-65 -0.2136686211434231 1101 66-67 -0.27213037476898094 1101 68-69 -0.17946573628842444 1101 70-71 -0.5638167617778223 1101 72-73 -0.6016891462999077 1101 74-75 -0.6289068593822869 1101 76-77 -0.4187141143880808 1101 78-79 -0.5721164468807345 1101 80-81 -0.06336998806516192 1101 82-83 0.025388023272206794 1101 84-85 -0.09545918186285363 1101 86-87 -0.010235905295445491 1101 88-89 -0.5426212106756942 1101 90-91 -0.3362068122507189 1101 92-93 -0.42765532447470633 1101 94-95 -0.43565137303643553 1101 96-97 -0.22254652681210985 1101 98-99 -0.050166585368423 1101 100-101 0.33469956733591033 1102 1 -0.11258045560196805 1102 2 -2.542430798762041E-4 1102 3 -0.01986844414032518 1102 4 0.04253256549511519 1102 5 -0.28400802054758145 1102 6 -0.09409221871574402 1102 7 -0.07337119047814866 1102 8 -0.04227630925877435 1102 9 0.04701584540190851 1102 10-11 -0.03851400173323327 1102 12-13 -0.22367369463385245 1102 14-15 -0.1552090090130207 1102 16-17 -0.22898450669676862 1102 18-19 0.02877156232930389 1102 20-21 0.07346369804937325 1102 22-23 -0.13003805806462765 1102 24-25 0.09144525164463602 1102 26-27 -0.08907393811115583 1102 28-29 -0.13402794593412892 1102 30-31 0.09920357068842378 1102 32-33 0.3598053891349622 1102 34-35 0.17751529631375007 1102 36-37 0.21935825623602767 1102 38-39 -0.1665611269943028 1102 40-41 0.09500378679500443 1102 42-43 0.1453328846910651 1102 44-45 0.35617683152509017 1102 46-47 0.2676670872805502 1102 48-49 0.41932975829138286 1102 50-51 0.4445662020254737 1102 52-53 0.5524507379549668 1102 54-55 0.40593956264028463 1102 56-57 0.438340653562026 1102 58-59 0.45802147936216997 1102 60-61 0.49528367286596264 1102 62-63 0.356333098820123 1102 64-65 0.4595696869419328 1102 66-67 0.543900244202046 1102 68-69 0.5026868615924407 1102 70-71 0.16355305402922937 1102 72-73 0.5687592613737316 1102 74-75 0.5316485614314139 1102 76-77 0.570657481003451 1102 78-79 0.7116019175085313 1102 80-81 0.7194980146786136 1102 82-83 1.0530113852286789 1102 84-85 0.7443298570276156 1102 86-87 0.7678955905226772 1102 88-89 0.6557302929690323 1102 90-91 0.5718045913742209 1102 92-93 0.753653607521656 1102 94-95 1.0615695141667736 1102 96-97 0.7509594629180789 1102 98-99 0.7926827882803344 1102 100-101 0.8418387364961255 1103 1 0.03355378399021447 1103 2 0.1577746439209733 1103 3 0.14325814889280863 1103 4 0.18951642275934688 1103 5 -0.03337080729354369 1103 6 -0.23767760529178616 1103 7 -0.10905513270414247 1103 8 -0.029532044178061767 1103 9 -0.01245739164142634 1103 10-11 -0.08396854718778002 1103 12-13 -0.1004791321869547 1103 14-15 0.006643157512044695 1103 16-17 0.13635109228368947 1103 18-19 -0.0341001454022134 1103 20-21 -0.1758990886965961 1103 22-23 -0.013215628158079085 1103 24-25 -0.08187675345306644 1103 26-27 0.15476633376649573 1103 28-29 -0.27591409716607984 1103 30-31 -0.047355477739777996 1103 32-33 0.050544556509649397 1103 34-35 0.04242608645818535 1103 36-37 -0.10859416517433118 1103 38-39 0.08492570393178056 1103 40-41 0.3099570578230413 1103 42-43 0.35476364085079837 1103 44-45 0.20069679754038816 1103 46-47 0.2387800864309355 1103 48-49 0.38322100722936625 1103 50-51 0.36512694968902437 1103 52-53 0.37742949751316957 1103 54-55 0.3634586790379082 1103 56-57 0.1558427776062068 1103 58-59 0.13049386678783037 1103 60-61 -0.025531960099204554 1103 62-63 0.058542104767447256 1103 64-65 0.05430205737523863 1103 66-67 0.3157778992572666 1103 68-69 0.40710487348708924 1103 70-71 0.12064736159082656 1103 72-73 0.4298467719939545 1103 74-75 0.26444380357245123 1103 76-77 0.35867787182758093 1103 78-79 0.41593496763597315 1103 80-81 0.24371211833197037 1103 82-83 0.1779051830196714 1103 84-85 0.23455925379906617 1103 86-87 0.34903407820322485 1103 88-89 0.33457481293504543 1103 90-91 0.4184486015696329 1103 92-93 0.48729846733474247 1103 94-95 0.5811107206238688 1103 96-97 0.6774675342859666 1103 98-99 0.7213149038283397 1103 100-101 1.072934743293068 1104 1 0.0912422287434893 1104 2 0.08224497202813907 1104 3 0.10805109364385146 1104 4 0.07935478708908761 1104 5 -0.0399237253805822 1104 6 0.12810586570143556 1104 7 -0.0015970965262042114 1104 8 0.2451957731377732 1104 9 0.2524284009953206 1104 10-11 0.11999772403220277 1104 12-13 0.06242129291947407 1104 14-15 -0.029630383667630156 1104 16-17 0.11368073352481645 1104 18-19 -0.07951302834394625 1104 20-21 -0.10780468739433502 1104 22-23 -0.2398139435046147 1104 24-25 0.18250574114940576 1104 26-27 0.09894972619612474 1104 28-29 0.16086080771691513 1104 30-31 0.10174795986390706 1104 32-33 0.10848260435309243 1104 34-35 0.061010915054147574 1104 36-37 -0.010570456228641945 1104 38-39 -0.13809409915332793 1104 40-41 0.011904557105111735 1104 42-43 0.5170019295928441 1104 44-45 0.49591066758608093 1104 46-47 0.34098083427304005 1104 48-49 0.505366567121456 1104 50-51 0.2876209123445399 1104 52-53 0.39062981831451893 1104 54-55 0.40096535619955986 1104 56-57 0.5032166257493245 1104 58-59 0.5843507739116731 1104 60-61 0.5274210936885915 1104 62-63 0.48803536957586857 1104 64-65 0.3752105382261064 1104 66-67 0.39730920491578203 1104 68-69 0.41247822168005044 1104 70-71 0.17042141685615064 1104 72-73 0.0970849342605149 1104 74-75 0.33256424394521034 1104 76-77 0.16622458963993836 1104 78-79 0.45721805399492155 1104 80-81 0.240920729973368 1104 82-83 0.1786279532196673 1104 84-85 0.28676498626323976 1104 86-87 0.5466817829707509 1104 88-89 0.7822474408129203 1104 90-91 0.8446163050872855 1104 92-93 0.3626248856829122 1104 94-95 0.5301980140038438 1104 96-97 0.6189596027850897 1104 98-99 0.9091153761026796 1104 100-101 0.6481846811712835 1105 1 -0.32415974060095465 1105 2 -0.2171303760054606 1105 3 -0.020981679975747625 1105 4 0.022412704927084803 1105 5 -0.17660524708862368 1105 6 -0.04776234669705559 1105 7 -0.18355705352565366 1105 8 -0.14067444124250983 1105 9 -0.07053500311395311 1105 10-11 -0.20035637439464438 1105 12-13 -0.07707057381919213 1105 14-15 -0.20693489182780667 1105 16-17 -0.1203383206313191 1105 18-19 -0.04364222514548288 1105 20-21 -0.11354482342815686 1105 22-23 -0.13790171442406063 1105 24-25 -0.3486456843793917 1105 26-27 -0.29765548467648983 1105 28-29 -0.2089624290670642 1105 30-31 -0.2407120551761892 1105 32-33 -0.1416065595419269 1105 34-35 -0.1547072496500732 1105 36-37 -0.3122236894292385 1105 38-39 -0.3384213979415378 1105 40-41 -0.09545339892194704 1105 42-43 -0.38854490584594004 1105 44-45 -0.07550035946422895 1105 46-47 -0.027968848690427706 1105 48-49 0.27558195680974507 1105 50-51 0.311480245364951 1105 52-53 0.18362321850394991 1105 54-55 0.014501276631715854 1105 56-57 0.09293148634241888 1105 58-59 0.17944196502354615 1105 60-61 -0.22228660014614476 1105 62-63 -0.01944724767044903 1105 64-65 -0.12611682617327702 1105 66-67 0.08134096072110708 1105 68-69 0.274321710444557 1105 70-71 0.12384505807409596 1105 72-73 0.07812709195948031 1105 74-75 0.22028082388737502 1105 76-77 0.19183818023634558 1105 78-79 0.2121956704925978 1105 80-81 0.07917153623368023 1105 82-83 0.16535557097909503 1105 84-85 0.020786143341421592 1105 86-87 0.2638017555150043 1105 88-89 0.33794879896409924 1105 90-91 0.171761579989667 1105 92-93 0.019086492214420048 1105 94-95 0.15248839893951782 1105 96-97 -0.06445599685892844 1105 98-99 0.3405180587582848 1105 100-101 0.6697295381809987 1106 1 -0.3792259263560638 1106 2 -0.2690549284737571 1106 3 -0.13479036659178334 1106 4 -0.10627342778275306 1106 5 -0.03267615133526647 1106 6 -0.2659910696835439 1106 7 -0.11358019286313237 1106 8 -0.1044097223884819 1106 9 -0.11939344232636984 1106 10-11 -0.5376590405538195 1106 12-13 -0.4937795317031899 1106 14-15 -0.4519264033763335 1106 16-17 -0.29507656820410233 1106 18-19 -0.1787128212704019 1106 20-21 -0.21515249384665935 1106 22-23 -0.17832154538002243 1106 24-25 -0.15159126766590703 1106 26-27 -0.20341252767457263 1106 28-29 -0.1135685467937293 1106 30-31 -0.3848344822087384 1106 32-33 -0.11495856142690286 1106 34-35 -0.4214575925110111 1106 36-37 -0.15500608903291635 1106 38-39 -0.09646277083528076 1106 40-41 -0.4175463308512164 1106 42-43 -0.6237006885657479 1106 44-45 -0.5384336178816866 1106 46-47 -0.33403945085694886 1106 48-49 -0.40282807466700987 1106 50-51 -0.4010950481058906 1106 52-53 -0.18442516264866882 1106 54-55 -0.33852768076894435 1106 56-57 0.014372529364667486 1106 58-59 -0.021829490217239567 1106 60-61 -0.14461424833155334 1106 62-63 -0.008803454510751152 1106 64-65 -0.03259584066707788 1106 66-67 0.10545299619739623 1106 68-69 0.25716204901596385 1106 70-71 -0.05212114520166011 1106 72-73 -0.0162336762906925 1106 74-75 0.22486488976500496 1106 76-77 0.24152280858717035 1106 78-79 0.2810412410917422 1106 80-81 0.5183341097146723 1106 82-83 0.17477745003829526 1106 84-85 0.12686308919053957 1106 86-87 0.3475111786023817 1106 88-89 0.22470651258005958 1106 90-91 0.10067155407119444 1106 92-93 -0.02589776941113442 1106 94-95 -0.3645836592531957 1106 96-97 -0.10412817950865616 1106 98-99 0.002473785895350744 1106 100-101 0.5513594416966079 1107 1 0.032181324673828726 1107 2 0.14407806013092994 1107 3 0.06983624369520314 1107 4 0.14796918495042632 1107 5 0.10211987025321179 1107 6 0.20255505923994122 1107 7 0.15737003091727786 1107 8 0.024273962898973878 1107 9 -0.13622147802233542 1107 10-11 -0.039895797507554676 1107 12-13 0.007389634560318825 1107 14-15 -0.08972309305980986 1107 16-17 -0.12754389123746535 1107 18-19 0.10020480198684112 1107 20-21 0.1421380143872426 1107 22-23 0.011932121288737108 1107 24-25 -0.05405410880798911 1107 26-27 0.022935547730106975 1107 28-29 -0.060522012615116694 1107 30-31 -0.06723279668252502 1107 32-33 -0.13489617098716167 1107 34-35 0.19816754126506453 1107 36-37 -0.07759712483149883 1107 38-39 -0.1720229548860317 1107 40-41 -0.07990074792261481 1107 42-43 -0.05472406770233107 1107 44-45 -0.0999418161823371 1107 46-47 -0.16561458431887388 1107 48-49 0.15852514234658344 1107 50-51 0.0745577058487541 1107 52-53 0.3881804288248496 1107 54-55 0.25974930430049525 1107 56-57 0.13831357783401543 1107 58-59 0.005707438263083731 1107 60-61 0.10462759996689996 1107 62-63 0.3891460802312352 1107 64-65 0.24105922544081793 1107 66-67 0.35452886791477667 1107 68-69 -0.057430591048376556 1107 70-71 -0.08242993494441464 1107 72-73 0.1393382151302589 1107 74-75 0.06814477549772491 1107 76-77 0.05433735253379268 1107 78-79 -0.48601821936394174 1107 80-81 -0.31465941667507735 1107 82-83 -0.3549684853680155 1107 84-85 -0.06953851758842333 1107 86-87 0.10087102630185285 1107 88-89 -0.06445139450237392 1107 90-91 0.24866978225810143 1107 92-93 0.11925529555325198 1107 94-95 0.23652539940303186 1107 96-97 0.13444980435234655 1107 98-99 0.3680793543311083 1107 100-101 1.048006400607715 1108 1 -0.01716477026196017 1108 2 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-1.4629897475948859 2316 84-85 -1.0274061950822677 2316 86-87 -1.8847523845552026 2316 88-89 -1.6447511570451283 2316 90-91 -1.106331913865091 2316 92-93 -0.8505661602495103 2316 94-95 -1.3329362915539846 2316 96-97 -1.0071827064390426 2316 98-99 -0.620231400334788 2316 100-101 -1.0053716854806503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 35.0 5 56.0 6 127.0 7 337.0 8 730.0 9 1254.0 10 1854.0 11 3013.0 12 4316.0 13 5982.0 14 8074.0 15 11163.0 16 14987.0 17 19043.0 18 15606.0 19 10075.0 20 8881.0 21 9357.0 22 9896.0 23 10900.0 24 12327.0 25 14486.0 26 17197.0 27 20416.0 28 23922.0 29 28782.0 30 33808.0 31 40405.0 32 47880.0 33 58000.0 34 71315.0 35 86209.0 36 90845.0 37 72318.0 38 23399.0 39 772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.864327360518196 22.511051916029874 16.946957234197722 17.677663489254204 2 17.84176028758701 24.8997791154848 36.74448624421753 20.513974352710658 3 21.422326564512936 27.75970644730786 31.44955784525741 19.3684091429218 4 14.30773661514409 19.830620919486435 36.18726069772764 29.674381767641833 5 12.14747278607573 40.44763931664598 32.7017173866917 14.703170510586586 6 28.854357647772773 39.53832822050394 15.86009961660208 15.747214515121202 7 25.989030345263842 37.01757047604598 19.608707838443163 17.38469134024701 8 27.624707180161025 34.21382855350214 18.560856383922484 19.60060788241435 9 27.6421927995248 16.311768593256208 20.02733413732897 36.018704469890025 10-11 25.299055519412896 28.641765944699156 25.26742711968135 20.791751416206598 12-13 27.323443853361407 26.481541601710035 27.045659825460856 19.149354719467702 14-15 23.187152698313923 26.794011689650826 28.178911314481436 21.839924297553814 16-17 19.79391397589556 30.232828736072577 28.554594989341485 21.41866229869038 18-19 19.75913559326392 26.75634046558033 34.19602150731182 19.28850243384393 20-21 21.3169628507731 25.054513989781196 34.82518237758138 18.80334078186433 22-23 20.68144649074536 25.586032924816017 34.85629464676023 18.876225937678395 24-25 20.49092225189353 25.842042940107817 34.98298364192492 18.68405116607374 26-27 20.088432483444624 25.957209632429183 35.09990440600256 18.85445347812363 28-29 19.566226425768495 26.599923369282912 35.04897422842125 18.784875976527342 30-31 19.84129229012757 26.07716558110113 34.95870308132613 19.12283904744517 32-33 19.884813482441096 26.254014621063487 34.7675826902654 19.093589206230025 34-35 20.291804130206152 26.221871938409475 34.65148332051912 18.834840610865257 36-37 20.51776718926383 25.9044950898838 34.54618389214458 19.031553828707786 38-39 19.917213306556334 26.457542087628667 34.63656911576766 18.988675490047342 40-41 20.03176982753522 26.431313658582994 34.275863931024375 19.261052582857406 42-43 19.946108974058113 26.777127625033675 33.681312569879275 19.595450831028938 44-45 20.52490286481302 26.557120118490786 33.22582677408323 19.692150242612968 46-47 20.920515002918556 27.51619348352109 32.058854537646795 19.50443697591356 48-49 20.922764990704337 27.786063447084143 31.394658143284364 19.896513418927153 50-51 20.8062156234009 28.162389975597275 31.081138416677167 19.950255984324656 52-53 21.283663031543544 28.563916367311148 30.233471589725657 19.918949011419652 54-55 20.757423031132117 28.405902939384042 30.350535239951554 20.486138789532284 56-57 21.21147056630264 28.53723794070832 30.138779246626946 20.11251224636209 58-59 21.51161893692577 28.03780493763034 30.201071765610415 20.249504359833477 60-61 20.996757446173863 28.365531729970613 30.841418289443574 19.796292534411954 62-63 20.928679244312672 28.18926125829603 31.025338719589808 19.856720777801492 64-65 20.700723338930445 28.55748783078035 30.667847803111925 20.07394102717728 66-67 20.964679048885163 28.399474402853244 30.25115006518536 20.384696483076233 68-69 20.971493297607815 28.48664535821091 30.16899336832172 20.37286797585956 70-71 21.15798514236637 28.658544425044553 30.093136637258254 20.090333795330825 72-73 21.22429064222448 28.54338174884704 29.9457687961007 20.286558812827778 74-75 20.790144282073896 29.44436873056975 29.40161896263992 20.363868024716435 76-77 20.926300685796274 28.808393611577536 29.755381327929936 20.50992437469625 78-79 20.806858477053982 28.95869279566768 29.659788989716912 20.574659737561422 80-81 20.82955121100771 29.213069986191503 29.534561098096894 20.42281770470389 82-83 20.96905356852424 28.92844308701888 29.459120483376072 20.64338286108081 84-85 20.59172563823003 29.02461634198021 29.35485281109214 21.02880520869762 86-87 20.777865777300068 28.876086101246877 29.089899226261345 21.25614889519171 88-89 20.581538271649382 28.95226425913688 29.082570694616226 21.38362677459751 90-91 20.80367900158285 28.76691984554674 29.055908943039256 21.373492209831156 92-93 20.807830547285878 28.967522077414472 29.065172488941204 21.15947488635845 94-95 20.55241700116485 28.67513004929402 29.16877736949428 21.60367558004685 96-97 20.511081511271794 28.5923947841426 29.184527283994743 21.71199642059086 98-99 20.604026845637584 28.68214662243822 28.75613926817352 21.957687263750675 100-101 20.530182822549744 28.373756258888456 29.0983985989348 21.997662319627 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1278.0 1 870.5 2 674.0 3 939.0 4 794.5 5 741.0 6 1407.5 7 3097.5 8 4607.5 9 4941.5 10 4186.5 11 3257.0 12 3120.0 13 3167.5 14 3276.0 15 3313.0 16 3322.0 17 3398.0 18 3465.0 19 3608.5 20 3770.0 21 4034.5 22 4471.5 23 5041.5 24 5598.5 25 6389.5 26 7397.0 27 8280.0 28 9273.5 29 10414.5 30 11775.0 31 13128.5 32 14840.5 33 16401.5 34 17572.0 35 18412.5 36 19599.5 37 21163.0 38 22036.0 39 22251.0 40 22058.5 41 22726.5 42 23505.5 43 24164.5 44 25226.5 45 25526.5 46 25226.5 47 26706.5 48 30159.0 49 29325.0 50 25104.5 51 22265.0 52 20123.0 53 19275.0 54 18994.5 55 17835.5 56 16046.0 57 14423.5 58 13239.0 59 11957.5 60 10295.5 61 8749.5 62 7400.5 63 5794.5 64 4267.5 65 3311.5 66 2449.5 67 1862.5 68 1388.5 69 885.5 70 613.0 71 477.5 72 394.5 73 338.0 74 277.0 75 221.5 76 169.0 77 118.5 78 82.5 79 49.0 80 30.0 81 16.5 82 8.0 83 8.0 84 4.0 85 1.0 86 1.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040499780144050646 2 0.0 3 0.0 4 0.0 5 0.01889989740055697 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002507129247012659 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001285707306160338 24-25 1.285707306160338E-4 26-27 0.0017357048633164563 28-29 0.003342838996016879 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01304992915752743 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.285707306160338E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.214268265400845E-4 84-85 7.714243836962028E-4 86-87 0.0 88-89 0.0 90-91 0.0091285218737384 92-93 9.642804796202535E-4 94-95 0.0 96-97 0.0 98-99 2.571414612320676E-4 100-101 0.011314224294210974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 777782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.18793769253881 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4324198745206 43.83711438802624 2 11.532724346392964 12.729345453079205 3 3.709693664135784 6.141910283841948 4 1.6342521450823364 3.607640222268075 5 0.921646021524872 2.5431871605245457 6 0.5502051397224926 1.821881218147172 7 0.3875077403287192 1.4970027120046503 8 0.26634837462803207 1.1759374002784655 9 0.20598367049933136 1.023103257587779 >10 1.2318551757293263 12.455718041549082 >50 0.08342944038070187 3.107057692242283 >100 0.039026840310465605 3.9983499034159062 >500 0.003038017508396272 1.1385534140418756 >1k 0.0011684682724601048 1.3126638704257148 >5k 4.673873089840419E-4 2.1674817259161485 >10k+ 2.3369365449202095E-4 1.4430532566509453 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11189 1.4385779048628022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9197 1.1824650094756628 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7609 0.9782946892574012 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3262 0.41939772326950225 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2211 0.2842698853920507 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2089 0.26858425625689464 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1526 0.19619893492006757 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1090 0.14014209637147684 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 953 0.12252790627708021 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 920 0.11828507216675109 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 903 0.11609936974627852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.285707306160338E-4 0.0 22-23 0.0 0.0 0.0 1.928560959240507E-4 0.0 24-25 0.0 0.0 0.0 7.714243836962028E-4 0.0 26-27 0.0 0.0 0.0 0.0014142780367763718 0.0 28-29 0.0 0.0 0.0 0.002507129247012659 0.0 30-31 0.0 0.0 0.0 0.005335685320565403 0.0 32-33 0.0 0.0 0.0 0.009449948700278484 0.0 34-35 0.0 0.0 0.0 0.0140784950024557 0.0 36-37 0.0 0.0 0.0 0.022821304684345997 0.0 38-39 0.0 0.0 0.0 0.03754265333988187 0.0 40-41 0.0 0.0 0.0 0.05798539950783124 0.0 42-43 0.0 0.0 0.0 0.07868528713701269 0.0 44-45 0.0 0.0 0.0 0.10465657472145151 0.0 46-47 0.0 0.0 0.0 0.14136351831232916 0.0 48-49 0.0 0.0 0.0 0.18244186674415197 0.0 50-51 0.0 0.0 0.0 0.23117017364762876 0.0 52-53 0.0 0.0 0.0 0.27957705372456554 0.0 54-55 0.0 0.0 0.0 0.33171248498936723 0.0 56-57 0.0 0.0 0.0 0.4079549282446753 0.0 58-59 0.0 0.0 0.0 0.4888902031674685 0.0 60-61 0.0 0.0 0.0 0.5954753388481605 0.0 62-63 0.0 0.0 0.0 0.7054675988901775 0.0 64-65 0.0 0.0 0.0 0.814495578452574 0.0 66-67 0.0 0.0 0.0 0.9444163017400762 0.0 68-69 0.0 1.285707306160338E-4 0.0 1.1005654540732492 0.0 70-71 0.0 1.285707306160338E-4 0.0 1.2943858304769202 0.0 72-73 0.0 1.285707306160338E-4 0.0 1.4882704922458991 0.0 74-75 0.0 1.285707306160338E-4 0.0 1.6939836612315533 0.0 76-77 0.0 1.285707306160338E-4 0.0 1.9221324227097054 0.0 78-79 0.0 1.285707306160338E-4 0.0 2.195280939903469 0.0 80-81 0.0 1.285707306160338E-4 0.0 2.5046221177656465 0.0 82-83 0.0 1.285707306160338E-4 0.0 2.8232846735974864 0.0 84-85 0.0 1.285707306160338E-4 0.0 3.1238830417777734 0.0 86-87 0.0 1.285707306160338E-4 0.0 3.4426098829749208 0.0 88-89 1.285707306160338E-4 1.285707306160338E-4 0.0 3.815400716396111 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATT 95 3.4900044E-5 29.999249 6 GGTATCA 8340 0.0 28.327497 1 GTATCAA 11925 0.0 28.262184 1 TAAGGTG 285 0.0 26.672857 5 GTAAGGT 245 1.0913936E-11 25.209936 4 GAGTACT 8525 0.0 23.763334 12-13 TACGCAT 140 1.7753287E-5 23.755514 5 AGTACTT 8590 0.0 23.389984 12-13 AGGACCG 185 2.5337613E-7 23.113472 5 CGCATTC 125 2.2553741E-4 22.79943 7 GTACTTT 8855 0.0 22.797283 14-15 TATCAAC 14840 0.0 22.699001 2 ATCAACG 14990 0.0 22.40847 3 TAGACTA 170 3.1404816E-6 22.358131 5 TCAACGC 15125 0.0 22.20846 4 CAACGCA 15200 0.0 22.130136 5 CTCTACG 130 2.9357118E-4 21.92817 2 AACGCAG 15600 0.0 21.587599 6 TACTTTT 9415 0.0 21.239511 14-15 AGGTAAG 250 9.451469E-9 20.904852 2 >>END_MODULE