FastQCFastQC Report
Thu 26 May 2016
SRR937156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879044
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133731.521311788715923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89561.0188340970417864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74600.8486492143737969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.24811044725861278No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.23536933304817506No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19350.2201254999749728No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13910.15824008809570397No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12800.14561273383357373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.14367881471234661No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12570.1429962550225017No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12240.1392421767283549No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.1282074617425294No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10790.12274698422377038No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.11046090980656258No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT8840.10056379430381188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89200.038.898321
GGTATCA52600.037.681061
ATCAACG114500.030.2099553
TATCAAC115350.029.9873432
TCAACGC116250.029.7568824
CAACGCA118050.029.3014835
AACGCAG120850.028.7372636
ACCGTGC1205.371967E-627.7135078
GTACATG73300.027.3641071
TACATGG73800.026.783122
ACATGGG74700.025.4427223
ACGCAGA136250.025.2799477
GCACCGT2451.0913936E-1125.2087846
CGCAGAG136750.025.1875158
CATGGGA52500.024.6183284
AGTACTT79950.024.33242212-13
GCAGAGT141000.024.1587649
GAGTACT79300.023.27382312-13
AGAGTAC121950.022.55455810-11
CTACGCC1302.93703E-421.927179
Produced by FastQC (version 0.11.5)