##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937156_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 879044 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35056265670433 33.0 31.0 34.0 31.0 34.0 2 32.44929150304194 34.0 31.0 34.0 31.0 34.0 3 32.47465542111658 34.0 31.0 34.0 31.0 34.0 4 36.052496803345456 37.0 35.0 37.0 35.0 37.0 5 36.08990107434895 37.0 37.0 37.0 35.0 37.0 6 36.16179849927876 37.0 37.0 37.0 35.0 37.0 7 36.081181374311186 37.0 37.0 37.0 35.0 37.0 8 36.10055583110743 37.0 37.0 37.0 35.0 37.0 9 37.81637551703896 39.0 38.0 39.0 35.0 39.0 10-11 37.74843011271336 39.0 38.0 39.0 35.0 39.0 12-13 37.62199503096546 39.0 38.0 39.0 35.0 39.0 14-15 38.86862887409504 40.0 38.0 41.0 36.0 41.0 16-17 38.84241346280732 40.0 38.0 41.0 36.0 41.0 18-19 38.899002211493396 40.0 38.5 41.0 36.0 41.0 20-21 38.83098513840035 40.0 38.5 41.0 36.0 41.0 22-23 38.70649023257084 40.0 38.0 41.0 35.0 41.0 24-25 38.640354180223056 40.0 38.0 41.0 35.0 41.0 26-27 38.63482715313454 40.0 38.0 41.0 35.0 41.0 28-29 38.525951488207646 40.0 38.0 41.0 35.0 41.0 30-31 38.22217431664399 40.0 38.0 41.0 35.0 41.0 32-33 37.88967787733037 40.0 37.0 41.0 34.0 41.0 34-35 37.864278693671764 40.0 37.0 41.0 34.0 41.0 36-37 37.720806922065336 40.0 37.0 41.0 34.0 41.0 38-39 37.58821572071477 40.0 37.0 41.0 33.5 41.0 40-41 37.336458698313166 40.0 36.5 41.0 33.0 41.0 42-43 36.99489274712074 40.0 36.0 41.0 33.0 41.0 44-45 36.614102934551624 40.0 36.0 41.0 31.5 41.0 46-47 36.05690215734366 40.0 35.0 41.0 30.5 41.0 48-49 35.56140818889612 39.0 35.0 41.0 27.5 41.0 50-51 34.51220530485391 38.0 34.0 40.0 25.0 40.5 52-53 34.60664540114033 38.5 35.0 40.0 25.5 40.5 54-55 34.9449925145954 39.0 35.0 40.0 25.5 41.0 56-57 34.73303384130942 39.0 35.0 40.5 23.5 41.0 58-59 34.4863857781863 38.0 35.0 40.0 23.0 41.0 60-61 34.194965212207805 38.0 34.5 40.0 22.0 41.0 62-63 33.89831680780484 37.5 34.0 40.0 21.0 41.0 64-65 33.501104609098064 37.0 34.0 40.0 19.0 41.0 66-67 33.146861818065986 36.5 34.0 39.5 16.5 41.0 68-69 32.69010652481559 36.0 33.0 39.0 10.0 41.0 70-71 32.241995281237344 35.0 33.0 39.0 7.0 40.0 72-73 31.81093210351245 35.0 32.5 38.0 2.0 40.0 74-75 31.360236233908658 35.0 32.0 37.0 2.0 39.5 76-77 30.91056192864066 35.0 31.5 37.0 2.0 39.0 78-79 30.438068515341666 35.0 31.0 36.0 2.0 39.0 80-81 29.985210637920286 34.5 31.0 36.0 2.0 37.5 82-83 29.542720273387907 34.0 30.0 35.0 2.0 37.0 84-85 29.05334431496034 34.0 29.5 35.0 2.0 36.5 86-87 28.65556388531177 34.0 29.0 35.0 2.0 36.0 88-89 28.243844449197084 34.0 29.0 35.0 2.0 36.0 90-91 27.787106788738676 34.0 28.0 35.0 2.0 35.5 92-93 27.354834911563017 34.0 27.0 35.0 2.0 35.0 94-95 26.908596156733907 33.0 25.5 35.0 2.0 35.0 96-97 26.281024613102417 33.0 24.5 35.0 2.0 35.0 98-99 25.513801356928663 33.0 22.0 35.0 2.0 35.0 100-101 23.11613127442995 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09617405642684673 1101 2 0.013438792344416584 1101 3 -0.07090271537641257 1101 4 0.08601249697086644 1101 5 0.12149412538450122 1101 6 -0.11164492962639372 1101 7 0.04083644836582323 1101 8 -0.010848730225795578 1101 9 0.05471870674573864 1101 10-11 -0.09982831836891393 1101 12-13 -0.029822503679859835 1101 14-15 -0.07907337669762171 1101 16-17 -0.040622498790177985 1101 18-19 0.10684445387366992 1101 20-21 0.1201716170171565 1101 22-23 0.16588758081867638 1101 24-25 0.12360887847126634 1101 26-27 0.13475950144680127 1101 28-29 0.04106803685587579 1101 30-31 0.07588616620120803 1101 32-33 0.13957749840239586 1101 34-35 0.3125050802045095 1101 36-37 0.2123145722785793 1101 38-39 0.2549383601170945 1101 40-41 0.23979287753578404 1101 42-43 0.23285042962531577 1101 44-45 0.31551917100718185 1101 46-47 0.1950541608221812 1101 48-49 0.2666802950880296 1101 50-51 0.33826806199669335 1101 52-53 0.496446137800973 1101 54-55 0.4354611639898849 1101 56-57 0.4463731772693862 1101 58-59 0.481404579020257 1101 60-61 0.3895324285921049 1101 62-63 0.2711432263031881 1101 64-65 0.21369509072570025 1101 66-67 0.32509722612221026 1101 68-69 0.46948626719022 1101 70-71 0.5034976541168632 1101 72-73 0.3277825164252697 1101 74-75 0.4783865768695499 1101 76-77 0.3840845812221687 1101 78-79 0.346343889361993 1101 80-81 0.6226400958590865 1101 82-83 0.6445059617784104 1101 84-85 0.46695793822003395 1101 86-87 0.35719987272121756 1101 88-89 0.42227716977864915 1101 90-91 0.3254072893006814 1101 92-93 0.4955177528984507 1101 94-95 0.49522046280917564 1101 96-97 0.4243434609864032 1101 98-99 0.4894121873384485 1101 100-101 0.9700819421464075 1102 1 0.1023892401572013 1102 2 0.1536811115072041 1102 3 0.13616776125650176 1102 4 0.025538062430634056 1102 5 0.13768827936211636 1102 6 -0.048601298074295585 1102 7 0.08762370773692396 1102 8 0.11789566495104964 1102 9 0.09586567858578121 1102 10-11 0.1283457432768884 1102 12-13 0.08858164088206166 1102 14-15 0.09729283135175137 1102 16-17 0.1080429207471596 1102 18-19 0.06114476869777263 1102 20-21 0.009102583846548384 1102 22-23 -0.08252772154105514 1102 24-25 0.1380451608091775 1102 26-27 0.045609941329502135 1102 28-29 0.16133706836732387 1102 30-31 0.18324160220563357 1102 32-33 0.1632573318235231 1102 34-35 0.395195976022201 1102 36-37 0.4057621658858608 1102 38-39 0.499845800169858 1102 40-41 0.5410612993088293 1102 42-43 0.44856840695300804 1102 44-45 0.48233944750487723 1102 46-47 0.49893574713749445 1102 48-49 0.5248692476238404 1102 50-51 0.5463481326765773 1102 52-53 0.636413037716764 1102 54-55 0.7025775991357008 1102 56-57 0.7379318262218391 1102 58-59 0.7590006335565889 1102 60-61 0.7987585585783847 1102 62-63 0.9870540800613767 1102 64-65 1.0030006111394911 1102 66-67 0.6824080724142121 1102 68-69 0.8306380505713236 1102 70-71 0.6924559969557507 1102 72-73 0.6219609665283379 1102 74-75 0.7301268200182882 1102 76-77 0.8119384265145335 1102 78-79 0.8690709546952569 1102 80-81 0.7810862169688946 1102 82-83 0.7034697383403987 1102 84-85 0.8278525530271352 1102 86-87 0.7386887128494735 1102 88-89 0.55987231178133 1102 90-91 0.642169288081206 1102 92-93 0.7094699776140061 1102 94-95 0.7611700992474759 1102 96-97 0.8429413115548705 1102 98-99 0.8092606237949944 1102 100-101 1.1560633513472354 1103 1 -0.04458759344849028 1103 2 -0.11247463028056615 1103 3 -0.09681573126751175 1103 4 -0.10099886310657524 1103 5 -0.09408052858664462 1103 6 -0.21029416639025555 1103 7 -0.12207104814643799 1103 8 -0.09878134255552595 1103 9 0.008696767519921877 1103 10-11 -0.13377270157730692 1103 12-13 -0.16054230063830488 1103 14-15 -0.26224069481639845 1103 16-17 -0.21894317592075652 1103 18-19 -0.16109881488388567 1103 20-21 -0.09515492928340308 1103 22-23 -0.21762424090172772 1103 24-25 -0.2438682732526729 1103 26-27 -0.3824571372230139 1103 28-29 -0.25370055189956275 1103 30-31 -0.3471211428067349 1103 32-33 -0.26276488204835147 1103 34-35 -0.23224540889298595 1103 36-37 -0.36361817020600284 1103 38-39 -0.21558156780965732 1103 40-41 -0.1030667057246788 1103 42-43 -0.31305192983796104 1103 44-45 -0.34246196467517365 1103 46-47 -0.1772590568089214 1103 48-49 -0.26554523071720126 1103 50-51 -0.2559510292784566 1103 52-53 -0.13027139771706686 1103 54-55 -0.05918255746248491 1103 56-57 -0.1745366050873045 1103 58-59 -0.07098721959766152 1103 60-61 -0.15757833724713066 1103 62-63 -0.12263455870540696 1103 64-65 -0.2103294597316676 1103 66-67 -0.10678298261361618 1103 68-69 -0.054836419205756215 1103 70-71 -0.20405986822299838 1103 72-73 -0.04142284758122372 1103 74-75 -0.06520756778942172 1103 76-77 -0.0596458192886864 1103 78-79 0.22372826508648913 1103 80-81 0.03864304061242407 1103 82-83 -0.03061046579651716 1103 84-85 0.031653030162228646 1103 86-87 0.26459470150659214 1103 88-89 0.2137658564756073 1103 90-91 0.08992911260734715 1103 92-93 0.04079048830585208 1103 94-95 -0.1994444088052738 1103 96-97 -0.2902676404082847 1103 98-99 -0.2892599078698197 1103 100-101 -0.04193460378808567 1104 1 0.06377584273498371 1104 2 0.08646047191747641 1104 3 -0.005796692920874591 1104 4 0.008105391328477651 1104 5 -0.0170088913858919 1104 6 0.008705205771960323 1104 7 -0.06326509248330581 1104 8 -0.0864247670921543 1104 9 -0.012905250053471207 1104 10-11 -0.07932180182813653 1104 12-13 -0.004851800400210493 1104 14-15 0.1379001137294722 1104 16-17 -0.0013443066353673316 1104 18-19 0.02496447541071234 1104 20-21 0.08567789601694642 1104 22-23 -0.01953611310185721 1104 24-25 -0.023428589658649912 1104 26-27 -0.03869964942339266 1104 28-29 -0.001643018301145105 1104 30-31 0.025644270307495276 1104 32-33 -0.03102601403683991 1104 34-35 0.062478554795539765 1104 36-37 0.027051614418340364 1104 38-39 -0.02515412250281912 1104 40-41 0.06805510693701677 1104 42-43 0.12659716343689809 1104 44-45 0.18079305575229654 1104 46-47 0.13210421862381594 1104 48-49 0.11921089828375386 1104 50-51 0.05631523763449309 1104 52-53 0.06519960194020058 1104 54-55 -0.05591332727652798 1104 56-57 0.11795694213549979 1104 58-59 0.09557746134245093 1104 60-61 0.14768958570171264 1104 62-63 0.211527171700439 1104 64-65 0.36420580382371526 1104 66-67 0.4033689571624848 1104 68-69 0.4507818948526534 1104 70-71 0.3167967815496766 1104 72-73 0.25002506159764337 1104 74-75 0.3317929144627847 1104 76-77 0.3981707610881955 1104 78-79 0.4503860449671713 1104 80-81 0.714701872348865 1104 82-83 0.6767287910497934 1104 84-85 0.6834646606296495 1104 86-87 0.8573285407497906 1104 88-89 0.5718472206124225 1104 90-91 0.8626093733268405 1104 92-93 0.6822700926770615 1104 94-95 0.8355632344372843 1104 96-97 0.9596796606475131 1104 98-99 1.0286428712980964 1104 100-101 1.0975238633778162 1105 1 0.016814366880396392 1105 2 -0.04416893665550248 1105 3 -0.1156718963934722 1105 4 -0.12077867450621227 1105 5 -0.12376418167346515 1105 6 -0.1230726925821699 1105 7 0.03172495996740565 1105 8 -0.028530030250045968 1105 9 0.05020742855025162 1105 10-11 -0.06246070578907137 1105 12-13 -0.05099034263208324 1105 14-15 -0.08909843935425954 1105 16-17 -0.12177295557730616 1105 18-19 -0.11466294365241225 1105 20-21 -0.11243206961879793 1105 22-23 -0.07321218472421265 1105 24-25 -0.014747092100321879 1105 26-27 -0.06998017031324366 1105 28-29 -0.22733482513782377 1105 30-31 -0.08143657613852184 1105 32-33 -0.12481331734665702 1105 34-35 0.012347428009505279 1105 36-37 -0.11170313327563264 1105 38-39 0.05561545608281904 1105 40-41 0.13569392046568396 1105 42-43 0.06032839874165319 1105 44-45 0.2283920790838252 1105 46-47 0.4296082880757979 1105 48-49 0.35010256042880883 1105 50-51 0.1941294897596535 1105 52-53 0.33071325721240186 1105 54-55 0.37691358536589803 1105 56-57 0.4680564176283539 1105 58-59 0.37160386756419683 1105 60-61 0.49956153361272726 1105 62-63 0.46516254880298646 1105 64-65 0.39453226430481436 1105 66-67 0.43169234294652625 1105 68-69 0.3168607517888091 1105 70-71 0.3337709179215338 1105 72-73 0.5415063421185273 1105 74-75 0.6378699627536157 1105 76-77 0.5961631647778312 1105 78-79 0.4652621527624312 1105 80-81 0.6846964464128895 1105 82-83 0.7944716016846307 1105 84-85 0.754557986728404 1105 86-87 0.7923846087548512 1105 88-89 0.8325589225476726 1105 90-91 1.0560665988649163 1105 92-93 1.1461604887703842 1105 94-95 0.9475771610062829 1105 96-97 0.9822018165527417 1105 98-99 0.7718966242371472 1105 100-101 0.9219677049404886 1106 1 -0.23420232358289894 1106 2 -0.0961153367999259 1106 3 -0.17462599351654262 1106 4 0.012075443951765408 1106 5 -0.03277900122937183 1106 6 -0.021289586547673878 1106 7 -0.31757509090814295 1106 8 -0.29128912630637416 1106 9 -0.44320993150095234 1106 10-11 -0.5519770598172684 1106 12-13 -0.3378656811186147 1106 14-15 -0.42206415579691736 1106 16-17 -0.3422946761556531 1106 18-19 -0.5831836959541121 1106 20-21 -0.5544573268685582 1106 22-23 -0.31602108958693265 1106 24-25 -0.33964287927640413 1106 26-27 -0.38878073815005365 1106 28-29 -0.019121295523326864 1106 30-31 -0.15773800557740714 1106 32-33 -0.06891831792106018 1106 34-35 0.03395887008522891 1106 36-37 -0.29235278124964026 1106 38-39 -0.27137935789318846 1106 40-41 -0.432343103806879 1106 42-43 -0.426953944159564 1106 44-45 -0.09109366755593129 1106 46-47 -0.20365775823736953 1106 48-49 -0.22287021294523157 1106 50-51 -0.2972946974623838 1106 52-53 -0.11983980927045934 1106 54-55 -0.11585429082597898 1106 56-57 -0.21530727692140061 1106 58-59 -0.4191691946516798 1106 60-61 -0.43245806168046386 1106 62-63 -0.3618002628097656 1106 64-65 -0.162850024438562 1106 66-67 -0.20582878681894812 1106 68-69 -0.1592765629667383 1106 70-71 -0.1565701123904475 1106 72-73 -0.2418122208932978 1106 74-75 -0.1044346997445409 1106 76-77 0.002184532509602377 1106 78-79 -0.08882184113250347 1106 80-81 -0.36374323323524393 1106 82-83 -0.29627766390216337 1106 84-85 -0.3982742224193494 1106 86-87 -0.23293992803145258 1106 88-89 0.17866735311895354 1106 90-91 0.31879574399152943 1106 92-93 -0.06194841773347903 1106 94-95 0.0892508025469887 1106 96-97 0.3302225934716496 1106 98-99 0.1631681848036699 1106 100-101 0.5968716978507302 1107 1 0.15014447523953578 1107 2 -0.011645071846423605 1107 3 0.0291455700667953 1107 4 0.11232891063310291 1107 5 0.10533798550920181 1107 6 0.1189519462207187 1107 7 0.1783838866959897 1107 8 0.15438405643725872 1107 9 0.22239202607347153 1107 10-11 0.1957565284730549 1107 12-13 0.20986008306451964 1107 14-15 0.31310099305027705 1107 16-17 0.14150426320494347 1107 18-19 0.14133550835997255 1107 20-21 0.09777013626355568 1107 22-23 0.08629665170509782 1107 24-25 0.22535223710504226 1107 26-27 0.19053808304072817 1107 28-29 0.06660383585289509 1107 30-31 0.0534673196303217 1107 32-33 0.07016378533651846 1107 34-35 0.18344359674015465 1107 36-37 0.06826977694108649 1107 38-39 0.23020265938519202 1107 40-41 0.22776048393317438 1107 42-43 0.15702280018644643 1107 44-45 0.29483944750487723 1107 46-47 0.42176998828219325 1107 48-49 0.3088728777662695 1107 50-51 0.256308604826728 1107 52-53 0.17067230004006007 1107 54-55 0.23391984181584746 1107 56-57 0.1994757287738551 1107 58-59 0.1459346854176502 1107 60-61 0.24029779522263084 1107 62-63 0.2963528867802907 1107 64-65 0.45479574469697326 1107 66-67 0.4345399951653164 1107 68-69 0.5104124158754999 1107 70-71 0.6846980980040911 1107 72-73 0.5989044329338107 1107 74-75 0.7186845912365847 1107 76-77 0.6529984975951706 1107 78-79 0.5398799933197047 1107 80-81 0.5308909768566501 1107 82-83 0.6067700407971159 1107 84-85 0.7114029944683402 1107 86-87 0.5846285458033549 1107 88-89 0.7425756924135136 1107 90-91 0.800688954386068 1107 92-93 0.7881012782440706 1107 94-95 0.7459066243705728 1107 96-97 0.8148159806394268 1107 98-99 0.9515712243403449 1107 100-101 1.055926683268659 1108 1 0.04421230096795625 1108 2 0.13909881215577258 1108 3 0.1120293661085725 1108 4 0.04474026302452927 1108 5 -0.05431017281955519 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-0.5895792849620527 2316 90-91 -0.4535884777406274 2316 92-93 -0.6279762104437125 2316 94-95 -0.33285468784661987 2316 96-97 0.06361405756250349 2316 98-99 0.3343837243403449 2316 100-101 0.49978980544060647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3430.0 3 624.0 4 356.0 5 391.0 6 447.0 7 473.0 8 600.0 9 715.0 10 982.0 11 1331.0 12 2265.0 13 3792.0 14 4188.0 15 6270.0 16 9476.0 17 15214.0 18 12545.0 19 6162.0 20 5346.0 21 4870.0 22 5187.0 23 5272.0 24 5676.0 25 6244.0 26 7077.0 27 8183.0 28 9922.0 29 11874.0 30 14888.0 31 19199.0 32 25399.0 33 36509.0 34 54320.0 35 89885.0 36 132058.0 37 189960.0 38 157018.0 39 20893.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67453495625835 19.114834527566195 15.215549242575438 20.99508127360002 2 14.81171529035112 20.94094468438311 41.00750676110166 23.23983326416411 3 18.834831698925417 27.029519664195867 30.35279042566047 23.782858211218247 4 11.459667020479735 16.688993753356186 37.977405368305725 33.873933857858354 5 12.222267826848356 38.92890694832084 33.136891406264255 15.711933818566557 6 29.329533441217425 37.20233513178167 17.756740760001232 15.71139066699967 7 26.829426838322085 35.431356650278886 20.07953261190617 17.659683899492855 8 27.215846633126407 32.6879569784573 19.598816816351107 20.49737957206519 9 27.77988718653901 15.837212935122569 20.637429121015067 35.74547075732335 10-11 24.411932401304536 27.50319292083837 26.180696512874313 21.90417816498278 12-13 26.36968903216757 25.452391229109494 27.632741054379316 20.545178684343618 14-15 22.953966417895558 25.54209050966057 28.373816835975823 23.130126236468048 16-17 20.053642109968607 29.563256979233067 28.348441083196583 22.03465982760174 18-19 19.73969717804786 26.444698335380046 33.54883656759219 20.266767918979905 20-21 21.31930212605854 25.04274064594099 33.620798262440964 20.017158965559506 22-23 20.498022151673233 25.37711373810769 33.910393409110476 20.21447070110861 24-25 20.19904507614537 25.566900533325427 33.9778917342539 20.256162656275293 26-27 19.72763752805517 25.884468281481503 34.08960438005556 20.298289810407763 28-29 19.53194221289183 26.333695576565407 33.92586519306272 20.208497017480042 30-31 19.620900909119417 25.938268824530546 33.981965853144004 20.458864413206037 32-33 19.512260480475824 26.10363709342687 33.81404481825936 20.57005760783795 34-35 20.10863615214079 26.17531407092483 33.53174693683913 20.18430284009525 36-37 20.05773601620348 25.85248698687885 33.61709186741923 20.47268512949844 38-39 19.827851091593278 26.27257537571707 33.4800496414562 20.41952389123345 40-41 19.779668533734338 26.4606393785207 33.472289835178934 20.287402252566032 42-43 19.57342399812103 26.5921350120913 32.8513916109988 20.983049378788863 44-45 19.653048017356152 26.739535135659082 32.71480661061838 20.892610236366384 46-47 20.151959219049356 27.11089316668187 31.955284645561537 20.781862968707234 48-49 20.24021184899145 27.38549650966926 31.29596704571244 21.078324595626853 50-51 20.148943085722106 27.75165508157086 31.16348955630699 20.93591227640005 52-53 20.42135520334894 27.928469992414694 30.58207244252562 21.068102361710743 54-55 19.87475637251939 27.903942631074152 30.823423820010472 21.39787717639599 56-57 20.393705859584095 28.088482347824055 30.30601361415982 21.21179817843203 58-59 20.62282447181724 27.65952353403528 30.559562805400407 21.158089188747073 60-61 20.349137103112742 27.780383779510593 31.144937836884303 20.725541280492358 62-63 20.26634959467301 27.91831444163371 31.07041437859171 20.74492158510157 64-65 20.06766565460764 28.082044606211763 30.982286212267933 20.868003526912656 66-67 20.137063050257023 28.140786003701297 30.52166374754135 21.20048719850033 68-69 20.14923416460331 27.99334710423478 30.75847115634533 21.098947574816577 70-71 20.278214774281807 28.185362517099865 30.44231646146831 21.094106247150023 72-73 20.233620062164164 28.252375331098555 30.39243906846095 21.121565538276332 74-75 19.892759346364397 28.914620160376476 30.059417272961493 21.133203220297634 76-77 19.92507971183452 28.44143339351508 30.24454844022482 21.38893845442558 78-79 20.064757387814666 28.48310379423568 30.269632433418458 21.182506384531194 80-81 20.125791899567126 28.790430056899346 29.89706588171184 21.186712161821692 82-83 20.14613784655583 28.62416216410326 29.89555800910982 21.33414198023109 84-85 20.06267734165079 28.64718174263074 29.662696037693042 21.627444878025422 86-87 19.964589380462368 28.71041956909859 29.751532826077536 21.573458224361502 88-89 20.05350245566353 28.54326938423724 29.478621818695878 21.924606341403354 90-91 19.9555231502516 28.566371237467724 29.568326006985636 21.90977960529504 92-93 20.15024273795416 28.526169927398065 29.702381664891092 21.62120566975668 94-95 20.021545827633382 28.242419060647517 29.800957592339262 21.935077519379846 96-97 19.931787463737507 28.20262874218386 29.86085577489293 22.004728019185702 98-99 20.026919419607736 28.285115533451453 29.365198811486533 22.322766235454278 100-101 20.044936058451075 27.792416384676372 29.98404667331246 22.178600883560094 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1976.0 1 1290.0 2 920.0 3 1230.5 4 981.0 5 879.0 6 1769.0 7 4011.5 8 6406.0 9 6733.0 10 4968.0 11 3475.5 12 3160.0 13 3194.5 14 3268.5 15 3190.5 16 3188.0 17 3254.0 18 3256.5 19 3329.0 20 3525.0 21 3856.0 22 4325.5 23 5051.0 24 5687.5 25 6408.5 26 7493.0 27 8692.5 28 9760.0 29 11001.5 30 12503.5 31 13983.0 32 16084.5 33 17845.0 34 19480.0 35 20446.0 36 21601.5 37 23667.5 38 24632.0 39 24726.5 40 24658.5 41 25589.0 42 26080.5 43 27039.0 44 28734.5 45 29317.5 46 29017.5 47 30555.0 48 34648.5 49 33775.5 50 28920.0 51 25517.5 52 23312.0 53 22772.0 54 22517.5 55 21054.5 56 18971.5 57 17026.0 58 15909.5 59 14573.5 60 12425.5 61 10618.0 62 9094.5 63 6847.0 64 4770.5 65 3821.0 66 2897.5 67 2160.5 68 1570.5 69 1005.5 70 749.5 71 591.0 72 503.5 73 446.0 74 353.0 75 277.0 76 200.5 77 140.5 78 94.0 79 67.5 80 50.5 81 30.5 82 19.0 83 12.5 84 8.0 85 6.0 86 4.5 87 3.5 88 4.0 89 2.0 90 3.5 91 4.5 92 3.0 93 2.0 94 1.0 95 1.5 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24924804674168757 2 0.2238795782691196 3 0.2122760635417567 4 0.21989798007835787 5 0.22069429971651022 6 0.24765540746538284 7 0.24697284777553796 8 0.2375307720660172 9 0.22990885552941603 10-11 0.23935093123893686 12-13 0.24122797038601024 14-15 0.22746301664080523 16-17 0.241284850360164 18-19 0.23059141521926094 20-21 0.22200253912204623 22-23 0.22234381896696864 24-25 0.21682646147405588 26-27 0.2249602977780407 28-29 0.2390096513940144 30-31 0.23849773162663074 32-33 0.23662069247955736 34-35 0.24708660772384544 36-37 0.22166125927712377 38-39 0.22234381896696864 40-41 0.2173952612155933 42-43 0.22376581832081213 44-45 0.24134173033431774 46-47 0.2461765281373856 48-49 0.2494755666383025 50-51 0.2501581263281474 52-53 0.2672221185742693 54-55 0.2494755666383025 56-57 0.22097869958727892 58-59 0.2064174262039215 60-61 0.20425598718607943 62-63 0.21056966431714452 64-65 0.20357342749623453 66-67 0.20289086780638965 68-69 0.20840822529930242 70-71 0.2097733446789922 72-73 0.2105127843429908 74-75 0.21136598395529688 76-77 0.22507405772634817 78-79 0.21887414054359056 80-81 0.2139824627663689 82-83 0.21142286392945064 84-85 0.2107971842137595 86-87 0.21682646147405588 88-89 0.21477878240452128 90-91 0.2227988587601986 92-93 0.21199166367098804 94-95 0.2097733446789922 96-97 0.2198411001042041 98-99 0.22547221754542435 100-101 0.2044835070826944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 879044.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.18188963150108 #Duplication Level Percentage of deduplicated Percentage of total 1 76.69940169279167 40.023197139354224 2 12.829880835551554 13.389748514921243 3 4.288612732175374 6.71363748787877 4 1.9533386443289318 4.0771560620527305 5 1.0470597732527225 2.7318778762729057 6 0.6726193470979066 2.105912912056517 7 0.43780399708523127 1.5991807899292092 8 0.3230824723625224 1.3487243131754916 9 0.22799368385203922 1.0707427122701814 >10 1.3737687928048552 13.305238519375836 >50 0.09435786752643105 3.381116663214223 >100 0.047939897321378495 4.70197414557748 >500 0.001307451741889812 0.4806472560688774 >1k 0.0021790862364830196 1.683573047872202 >5k 4.3581724729660395E-4 1.866644276230587 >10k+ 2.1790862364830197E-4 1.5206282837494907 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13373 1.521311788715923 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8956 1.0188340970417864 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7460 0.8486492143737969 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2181 0.24811044725861278 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2069 0.23536933304817506 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1935 0.2201254999749728 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1391 0.15824008809570397 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1280 0.14561273383357373 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.14367881471234661 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1257 0.1429962550225017 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1224 0.1392421767283549 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1127 0.1282074617425294 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1079 0.12274698422377038 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 971 0.11046090980656258 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 884 0.10056379430381188 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.6879974153739746E-5 0.0 22-23 0.0 0.0 0.0 1.1375994830747949E-4 0.0 24-25 0.0 0.0 0.0 1.1375994830747949E-4 0.0 26-27 0.0 0.0 0.0 3.412798449224385E-4 0.0 28-29 0.0 0.0 0.0 6.82559689844877E-4 0.0 30-31 0.0 0.0 0.0 0.00221831899199585 0.0 32-33 0.0 0.0 0.0 0.0051191976738365775 0.0 34-35 0.0 0.0 0.0 0.009385195735367058 0.0 36-37 0.0 0.0 0.0 0.016324552582123307 0.0 38-39 0.0 0.0 0.0 0.030089506327328326 0.0 40-41 0.0 0.0 0.0 0.046186539012836675 0.0 42-43 0.0 0.0 0.0 0.06325053125895859 0.0 44-45 0.0 0.0 0.0 0.08554748112722457 0.0 46-47 0.0 0.0 0.0 0.11467002789393932 0.0 48-49 0.0 0.0 0.0 0.14777417285141586 0.0 50-51 0.0 0.0 0.0 0.18980847375102952 0.0 52-53 0.0 0.0 0.0 0.23377669377187035 0.0 54-55 0.0 0.0 0.0 0.28081643239701315 0.0 56-57 0.0 0.0 0.0 0.34662656249289003 0.0 58-59 0.0 0.0 0.0 0.4235851675228999 0.0 60-61 0.0 0.0 0.0 0.5115216075645815 0.0 62-63 0.0 0.0 0.0 0.6040653255127161 0.0 64-65 0.0 0.0 0.0 0.7011594413931498 0.0 66-67 0.0 0.0 0.0 0.815431309468013 0.0 68-69 0.0 0.0 0.0 0.9551854059637515 0.0 70-71 0.0 0.0 0.0 1.1311151660212686 0.0 72-73 0.0 0.0 0.0 1.3055091667766345 0.0 74-75 0.0 0.0 0.0 1.4855911649473748 0.0 76-77 0.0 0.0 0.0 1.695136989729752 0.0 78-79 0.0 0.0 0.0 1.9417116776862136 0.0 80-81 0.0 0.0 0.0 2.220480431013692 0.0 82-83 0.0 0.0 0.0 2.5131847780088368 0.0 84-85 0.0 0.0 0.0 2.8050359253916755 0.0 86-87 0.0 0.0 0.0 3.117477623418168 0.0 88-89 0.0 0.0 0.0 3.4653555453424403 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8920 0.0 38.89832 1 GGTATCA 5260 0.0 37.68106 1 ATCAACG 11450 0.0 30.209955 3 TATCAAC 11535 0.0 29.987343 2 TCAACGC 11625 0.0 29.756882 4 CAACGCA 11805 0.0 29.301483 5 AACGCAG 12085 0.0 28.737263 6 ACCGTGC 120 5.371967E-6 27.713507 8 GTACATG 7330 0.0 27.364107 1 TACATGG 7380 0.0 26.78312 2 ACATGGG 7470 0.0 25.442722 3 ACGCAGA 13625 0.0 25.279947 7 GCACCGT 245 1.0913936E-11 25.208784 6 CGCAGAG 13675 0.0 25.187515 8 CATGGGA 5250 0.0 24.618328 4 AGTACTT 7995 0.0 24.332422 12-13 GCAGAGT 14100 0.0 24.158764 9 GAGTACT 7930 0.0 23.273823 12-13 AGAGTAC 12195 0.0 22.554558 10-11 CTACGCC 130 2.93703E-4 21.92717 9 >>END_MODULE