FastQCFastQC Report
Thu 26 May 2016
SRR937156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879044
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131421.4950332406568954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110871.2612565468850252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86910.9886877107403041No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35840.4077156547340065No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.2928181069434522No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.27086243692010864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16500.18770391470734116No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12400.14106233590127457No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10410.11842410618808615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.10977835011671772No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9590.10909579042687283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96950.030.2857691
GTATCAA135000.027.3806481
TAAGGTG3300.025.9079445
AGGTAAG2950.024.1514722
GTAAGGT3350.024.1034094
GAGTACT97900.023.89449712-13
AGTACTT98500.023.86950112-13
GTACTTT101550.022.80179614-15
GGTAAGG3650.022.1223093
TATCAAC168400.021.9720082
ATCAACG171450.021.525733
TCAACGC172050.021.478274
GATATAG3101.0913936E-1121.4567641
CAACGCA173400.021.2836595
TACTTTT110250.021.26096314-15
TGGTACT2701.0004442E-921.1101762
AGAGTAC147100.021.10120610-11
AACGCAG176950.020.9371916
ACTTTTT115500.020.33567816-17
GTACATG73700.020.3067761
Produced by FastQC (version 0.11.5)