##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937156_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 879044 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.255815408557478 31.0 31.0 33.0 30.0 34.0 2 31.479741628405403 31.0 31.0 34.0 30.0 34.0 3 31.423557865135304 31.0 31.0 34.0 28.0 34.0 4 35.30866031734475 37.0 35.0 37.0 33.0 37.0 5 35.19526326327237 37.0 35.0 37.0 32.0 37.0 6 35.32588243591902 37.0 35.0 37.0 33.0 37.0 7 35.23288936617507 37.0 35.0 37.0 32.0 37.0 8 35.21435332019785 37.0 35.0 37.0 32.0 37.0 9 36.703213946059584 39.0 35.0 39.0 32.0 39.0 10-11 36.57496041153799 38.0 35.0 39.0 32.0 39.0 12-13 36.422997597389895 38.0 35.0 39.0 32.0 39.0 14-15 37.14445181356109 39.0 36.0 40.0 32.0 41.0 16-17 37.121039447399674 38.5 36.0 40.0 31.5 41.0 18-19 37.24542741887778 39.0 36.0 40.0 32.0 41.0 20-21 37.183993633993296 39.0 36.0 40.0 31.0 41.0 22-23 36.971715864052314 39.0 36.0 40.0 31.0 41.0 24-25 36.827363590445984 38.5 35.5 40.0 31.0 41.0 26-27 36.638147237225894 38.0 35.0 40.0 30.5 41.0 28-29 36.400115921387325 38.0 35.0 40.0 30.0 41.0 30-31 36.26876811627177 38.0 35.0 40.0 30.0 41.0 32-33 35.99091626812765 38.0 35.0 40.0 30.0 41.0 34-35 35.70530940430741 38.0 34.5 40.0 29.5 41.0 36-37 35.33495194779783 38.0 34.0 40.0 28.5 41.0 38-39 35.08025764353093 38.0 34.0 40.0 27.0 41.0 40-41 34.728142732331946 37.5 33.0 40.0 26.0 41.0 42-43 34.28153880806876 37.0 33.0 40.0 24.5 41.0 44-45 33.64123809502141 37.0 33.0 40.0 23.0 41.0 46-47 32.87371223738516 37.0 32.0 40.0 17.5 41.0 48-49 32.19912484471767 36.5 31.0 39.0 10.5 40.0 50-51 31.747240752453802 36.0 30.5 39.0 7.5 40.0 52-53 31.352251991936697 36.0 30.0 39.0 4.5 40.0 54-55 31.0068659816801 35.0 29.5 39.0 2.0 40.0 56-57 30.59064051401295 35.0 28.5 38.5 2.0 40.0 58-59 30.1801485477405 35.0 28.0 38.0 2.0 40.0 60-61 29.68232420675188 34.0 27.5 38.0 2.0 40.0 62-63 29.176992277974712 34.0 26.5 38.0 2.0 40.0 64-65 28.632683915708427 34.0 26.0 37.0 2.0 39.5 66-67 28.044721310878636 33.0 25.5 36.5 2.0 39.0 68-69 27.459948534999384 33.0 24.0 36.0 2.0 39.0 70-71 26.943436278502553 32.0 23.0 35.5 2.0 38.5 72-73 26.330701307329328 31.5 21.5 35.0 2.0 38.0 74-75 25.71661771196891 31.0 20.0 35.0 2.0 37.5 76-77 24.922822975869238 30.5 20.0 34.0 2.0 36.0 78-79 25.453364109191348 31.0 20.0 34.5 2.0 36.0 80-81 25.154848335236917 31.0 19.0 34.0 2.0 36.0 82-83 24.6611170771884 31.0 17.5 34.0 2.0 36.0 84-85 24.07435748381196 31.0 10.5 34.0 2.0 35.0 86-87 23.428588330049465 30.0 7.0 34.0 2.0 35.0 88-89 22.626688766432622 29.5 2.0 34.0 2.0 35.0 90-91 21.744633374438592 29.0 2.0 34.0 2.0 35.0 92-93 20.980754660745085 28.5 2.0 33.0 2.0 35.0 94-95 20.123510313476913 27.0 2.0 33.0 2.0 35.0 96-97 19.166609976292428 25.5 2.0 33.0 2.0 34.0 98-99 17.934493040166362 24.5 2.0 32.5 2.0 34.0 100-101 15.64875592120531 10.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08166160905917152 1101 2 0.004421621589848712 1101 3 -0.00775411937451409 1101 4 -0.04107638282933124 1101 5 0.06860997241392397 1101 6 -0.043882371014184685 1101 7 -0.09920748829007664 1101 8 -0.11413276188415011 1101 9 0.019704056622650512 1101 10-11 -0.08583168572329924 1101 12-13 0.0260262918140981 1101 14-15 -0.10793664278942572 1101 16-17 0.021204752018185502 1101 18-19 -0.06104000752809213 1101 20-21 0.08449241675000962 1101 22-23 0.10971741796636536 1101 24-25 -0.025345987259058234 1101 26-27 0.12932392233216916 1101 28-29 -0.25144256968808065 1101 30-31 -0.2163585113216655 1101 32-33 -0.03919758596871503 1101 34-35 -0.0718108614614863 1101 36-37 0.09981451798696384 1101 38-39 -0.005833759906501257 1101 40-41 0.16176294807537062 1101 42-43 0.06784629897452987 1101 44-45 0.1073519061871906 1101 46-47 0.1618155794794518 1101 48-49 0.08911394620935198 1101 50-51 0.32287238334426505 1101 52-53 0.3909982497446798 1101 54-55 0.3098589590651919 1101 56-57 0.29668330322151704 1101 58-59 0.2992111523204848 1101 60-61 0.3507835815203286 1101 62-63 0.3575395626277391 1101 64-65 0.3017299430952143 1101 66-67 0.5373267575440686 1101 68-69 0.5708873341185736 1101 70-71 0.6866744999298611 1101 72-73 0.7361636618903908 1101 74-75 0.4840300932927768 1101 76-77 0.8202108375129846 1101 78-79 0.5813078553797588 1101 80-81 0.4128458904340988 1101 82-83 0.7053307491717327 1101 84-85 0.7112677202845674 1101 86-87 0.6744534698841491 1101 88-89 0.836084458709756 1101 90-91 0.4135471361152554 1101 92-93 0.6594221020988336 1101 94-95 0.9317605996054397 1101 96-97 0.7574879118998616 1101 98-99 0.8663774630341656 1101 100-101 1.4724632352840317 1102 1 -0.003102416734407143 1102 2 0.005252027211056998 1102 3 -0.16138828463435928 1102 4 0.018073218766772925 1102 5 -0.05883364752410358 1102 6 0.030396208917544243 1102 7 -0.09625004968907547 1102 8 0.07589822278114156 1102 9 -0.1974142450692682 1102 10-11 -0.051380222143755816 1102 12-13 -0.028469388168645082 1102 14-15 -0.06802076180040473 1102 16-17 -0.18368601355606273 1102 18-19 -0.21315190074506063 1102 20-21 0.040643515730877766 1102 22-23 0.09440925120498633 1102 24-25 0.07881018872720347 1102 26-27 0.18331024262318607 1102 28-29 0.048228918198034876 1102 30-31 0.08842897203496847 1102 32-33 0.054897076953800195 1102 34-35 0.03902796435657763 1102 36-37 0.13875066966664917 1102 38-39 0.1814027978506516 1102 40-41 0.08738772253438754 1102 42-43 0.3288956297140544 1102 44-45 0.007560959350577434 1102 46-47 0.03541764014635618 1102 48-49 0.42796008888814185 1102 50-51 0.43947137043191375 1102 52-53 0.4285602087055551 1102 54-55 0.45072843771813 1102 56-57 0.4645762215456024 1102 58-59 0.6558064892735338 1102 60-61 0.770028501404024 1102 62-63 0.6723259260567112 1102 64-65 0.3675480917303524 1102 66-67 0.7471929054491824 1102 68-69 0.715613511805465 1102 70-71 0.7612164702559276 1102 72-73 0.8849688649913467 1102 74-75 0.7617327483509051 1102 76-77 0.8608310285643483 1102 78-79 0.7479970588623175 1102 80-81 0.7041564961490465 1102 82-83 0.6715329915157788 1102 84-85 0.7371355605248091 1102 86-87 0.8891751263815948 1102 88-89 0.9831069028896557 1102 90-91 1.1476738206731127 1102 92-93 1.0996830867226421 1102 94-95 1.0259701588102068 1102 96-97 1.1472808082401862 1102 98-99 1.6178729812495796 1102 100-101 1.9886739807825435 1103 1 0.10482099696618619 1103 2 0.09595138840931128 1103 3 0.13105358091141994 1103 4 -0.06353785496196451 1103 5 0.0066806708980280405 1103 6 -0.12049557929040589 1103 7 -0.1996992295585187 1103 8 -0.17354031330548025 1103 9 -0.024850386741690045 1103 10-11 -0.11009580243965189 1103 12-13 -0.03633862169393609 1103 14-15 -0.11327868565103927 1103 16-17 -0.20177083174662158 1103 18-19 -0.09389434392141993 1103 20-21 -0.2515580345212385 1103 22-23 -0.02492695426022351 1103 24-25 -0.0957024081635538 1103 26-27 -0.2881184536445005 1103 28-29 -0.30345285192072424 1103 30-31 -0.5323098008714524 1103 32-33 -0.14423254474623803 1103 34-35 -0.052942883213610514 1103 36-37 0.16894300928895944 1103 38-39 -0.03502952499327705 1103 40-41 0.10390830663163086 1103 42-43 0.4159771534004051 1103 44-45 0.3400000285002136 1103 46-47 0.49379503128352553 1103 48-49 0.4010491453665992 1103 50-51 0.7979212930940811 1103 52-53 0.930006469836524 1103 54-55 0.5759763994075016 1103 56-57 0.6571080960922835 1103 58-59 0.7750247217763935 1103 60-61 0.8116598490048013 1103 62-63 0.9519087387069085 1103 64-65 0.7014159445350501 1103 66-67 0.9221031149167338 1103 68-69 0.9366948432395787 1103 70-71 0.9717982080413599 1103 72-73 1.0092468267681092 1103 74-75 1.1520091546323137 1103 76-77 1.2563637857235577 1103 78-79 1.2179838633680085 1103 80-81 0.7089628020223557 1103 82-83 0.8437866246460857 1103 84-85 0.8750765390886848 1103 86-87 1.0535245710347994 1103 88-89 1.3360645287493291 1103 90-91 1.3327034886884555 1103 92-93 1.6050137168730352 1103 94-95 1.7279791946652523 1103 96-97 2.1856526065850694 1103 98-99 2.3455258436231965 1103 100-101 2.624390950537773 1104 1 -0.043297157858006585 1104 2 0.09574741695621469 1104 3 0.11898666007660808 1104 4 0.06190079740092358 1104 5 -0.08261257180829062 1104 6 -0.0436392874155942 1104 7 0.008794475381989741 1104 8 0.04073207730061057 1104 9 0.00819677995075807 1104 10-11 -0.11904704037197433 1104 12-13 -0.035070434871251166 1104 14-15 0.046252392319132696 1104 16-17 -0.21080547807984118 1104 18-19 -0.24891037349955525 1104 20-21 -0.13410308264030135 1104 22-23 -0.3019129841113255 1104 24-25 -0.15866665174034722 1104 26-27 -0.43024106096078185 1104 28-29 -0.28011139005959507 1104 30-31 -0.20193898228622942 1104 32-33 -0.11339153025743798 1104 34-35 0.009613038327891843 1104 36-37 -0.0639976979536172 1104 38-39 -0.23825295638455657 1104 40-41 -0.050711912171351514 1104 42-43 -0.05824282639959222 1104 44-45 -0.04025090946517196 1104 46-47 -0.16488679751390833 1104 48-49 -0.019028166638449306 1104 50-51 -0.036158924556346506 1104 52-53 0.03705546544772531 1104 54-55 0.19110311951620318 1104 56-57 0.09029265916874962 1104 58-59 0.06003312327714028 1104 60-61 0.1861063205261395 1104 62-63 0.28510975598609534 1104 64-65 0.38031483232193963 1104 66-67 0.6970790023062925 1104 68-69 0.266250534270597 1104 70-71 0.16549336701776696 1104 72-73 0.36494650193030154 1104 74-75 0.446995475654564 1104 76-77 0.5819473412265417 1104 78-79 0.31591480424510365 1104 80-81 0.5557893398457878 1104 82-83 0.428255828747961 1104 84-85 0.46422154231886736 1104 86-87 0.3553583426413667 1104 88-89 0.6230925509389991 1104 90-91 0.5271000364905696 1104 92-93 0.8593714266906609 1104 94-95 1.2349650273692276 1104 96-97 1.1360807191591142 1104 98-99 1.3331700264180135 1104 100-101 1.7214984230205843 1105 1 -0.22287755214268756 1105 2 -0.15483134954767408 1105 3 -0.17056841317352678 1105 4 -0.15020808304762312 1105 5 -0.07081116106493113 1105 6 -0.08845751856046746 1105 7 -0.12364357851564023 1105 8 -0.3264862261900703 1105 9 -0.07297522221961827 1105 10-11 -0.16163611615421303 1105 12-13 -0.13014639797489025 1105 14-15 -0.15047848125656316 1105 16-17 -0.04962352836017914 1105 18-19 -0.09838134474209426 1105 20-21 -0.14649230311417227 1105 22-23 -0.10375780234247145 1105 24-25 -0.10348488288521906 1105 26-27 0.025067313067076213 1105 28-29 0.028683147831529254 1105 30-31 -0.10318242594693317 1105 32-33 -0.15003920614820032 1105 34-35 -0.07239094069182528 1105 36-37 -0.1908334729581398 1105 38-39 -0.21306757023878475 1105 40-41 -0.26196168609068593 1105 42-43 0.008211324441425916 1105 44-45 0.22107573188046103 1105 46-47 0.36633494240289366 1105 48-49 0.5901326219309126 1105 50-51 0.6289884800376093 1105 52-53 0.41679863781162396 1105 54-55 0.7912297246889288 1105 56-57 0.7778943954682624 1105 58-59 0.664165877843967 1105 60-61 0.8393618821105129 1105 62-63 0.7154181300139477 1105 64-65 0.7017744090989311 1105 66-67 0.9835774649577651 1105 68-69 1.0605126069781363 1105 70-71 1.175553956636147 1105 72-73 1.1229633223318167 1105 74-75 1.3388384852403874 1105 76-77 1.2483517543211313 1105 78-79 1.1728916535849478 1105 80-81 1.0677897015748528 1105 82-83 0.9551549063388443 1105 84-85 1.1337594334384171 1105 86-87 0.9629003936451639 1105 88-89 1.2618201689531041 1105 90-91 1.465497576732929 1105 92-93 1.7316268074105032 1105 94-95 1.609060704706902 1105 96-97 1.6672481997161768 1105 98-99 1.6669555210825102 1105 100-101 1.6391824526823697 1106 1 -0.43628736291845627 1106 2 -0.11958936244708696 1106 3 -0.13162974283069317 1106 4 -0.010690087872589515 1106 5 -0.2072971374652397 1106 6 -0.17802556871392028 1106 7 -0.17377651140915162 1106 8 -0.139605553976331 1106 9 -0.18719329781084326 1106 10-11 -0.24221109194729706 1106 12-13 -0.26983917528767876 1106 14-15 -0.28051146830791396 1106 16-17 -0.17099002927101026 1106 18-19 0.03491383192736208 1106 20-21 -0.08833416220529955 1106 22-23 -0.12662431145904662 1106 24-25 -0.18680098417680568 1106 26-27 0.09173808260081984 1106 28-29 -0.36714957007174576 1106 30-31 -0.2685964872930029 1106 32-33 -0.27664547861139255 1106 34-35 -0.029833131294616066 1106 36-37 -0.298009797773922 1106 38-39 -0.35941068480724425 1106 40-41 -0.2719603731732221 1106 42-43 -0.18657598292896438 1106 44-45 0.13803780497714513 1106 46-47 -0.0646055988473293 1106 48-49 -0.2027234659032615 1106 50-51 -0.005387802108348438 1106 52-53 0.09439821439574203 1106 54-55 0.08955616520556475 1106 56-57 -0.03800995849428901 1106 58-59 0.05736619622645378 1106 60-61 0.25718432643460787 1106 62-63 0.5122260994229713 1106 64-65 0.5434373831852248 1106 66-67 0.39187879592784824 1106 68-69 0.5503198542825558 1106 70-71 0.7448144558730085 1106 72-73 0.7665960829049077 1106 74-75 0.3634126076371196 1106 76-77 0.4671826833743573 1106 78-79 0.6644528765675695 1106 80-81 0.38883576856646584 1106 82-83 0.5139149378488632 1106 84-85 0.6602557490637757 1106 86-87 0.869967171909817 1106 88-89 1.1210930588485617 1106 90-91 1.2102717549286481 1106 92-93 1.1986003685350148 1106 94-95 1.2868851184071985 1106 96-97 1.2251802726487426 1106 98-99 1.1617079540826936 1106 100-101 1.5959013078183375 1107 1 -0.01598578227684655 1107 2 -0.18527833097182267 1107 3 -0.10631402720861516 1107 4 -0.1447901263912712 1107 5 -0.16554477819600066 1107 6 -0.09155026881846595 1107 7 -0.0729469840883965 1107 8 0.016076983304252224 1107 9 0.0691364908866774 1107 10-11 0.09939974555889108 1107 12-13 -0.033208510977985384 1107 14-15 -0.06537302460021976 1107 16-17 -0.08079612897936528 1107 18-19 -0.025264729675015474 1107 20-21 0.10849028202515854 1107 22-23 0.205905832848444 1107 24-25 -0.1214060645157744 1107 26-27 -0.0474317194406737 1107 28-29 -0.12095243008385381 1107 30-31 0.04814787591065084 1107 32-33 0.04128238827905051 1107 34-35 0.21270645352903017 1107 36-37 0.05674940887880098 1107 38-39 -0.1742953179168012 1107 40-41 -0.3573402980617928 1107 42-43 -0.30534807958334653 1107 44-45 -0.23742691259838722 1107 46-47 -0.41400400238554624 1107 48-49 -0.10689233936939502 1107 50-51 0.049333229779616516 1107 52-53 -0.12007732968063678 1107 54-55 0.2011739326352675 1107 56-57 -0.04704594250903682 1107 58-59 -0.14959992452961401 1107 60-61 0.21743528252331146 1107 62-63 0.010596793584639386 1107 64-65 -0.031631486153909094 1107 66-67 -0.019287924619117547 1107 68-69 0.2975336983094081 1107 70-71 0.4184670758311917 1107 72-73 0.6987615034463701 1107 74-75 0.9398446771908517 1107 76-77 0.7998271800177257 1107 78-79 1.0569715684456291 1107 80-81 1.0643567165387324 1107 82-83 0.7511930255338903 1107 84-85 0.6406447533558612 1107 86-87 0.7771354297648756 1107 88-89 0.9607672148767357 1107 90-91 0.8450673651746214 1107 92-93 0.9021182934786118 1107 94-95 1.177430157751278 1107 96-97 1.5258766181888284 1107 98-99 1.7035428533838548 1107 100-101 2.0244298082035215 1108 1 -0.021172354569312546 1108 2 0.08476530564170304 1108 3 0.18212055223063572 1108 4 -0.001634546402272008 1108 5 -0.11849110859731837 1108 6 -0.03424371301857576 1108 7 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100-101 -0.979635519850774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 17.0 4 28.0 5 66.0 6 152.0 7 360.0 8 865.0 9 1486.0 10 2479.0 11 3638.0 12 5287.0 13 7158.0 14 9435.0 15 13120.0 16 17563.0 17 22371.0 18 18658.0 19 12698.0 20 11461.0 21 11888.0 22 12613.0 23 13826.0 24 15617.0 25 18360.0 26 21466.0 27 25332.0 28 30121.0 29 35423.0 30 41191.0 31 47985.0 32 56487.0 33 66895.0 34 79202.0 35 92684.0 36 91952.0 37 69041.0 38 21442.0 39 674.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92397048462713 22.50029308223489 16.93810444654123 17.63763198659675 2 18.012522695109688 24.87907317494915 36.60829264519182 20.500111484749343 3 21.439882417717428 27.732741478242275 31.415492284800305 19.411883819239993 4 14.285177988815123 19.80424188095249 36.195230272887365 29.715349857345025 5 12.189472702265796 40.50355562785756 32.6447705365807 14.662201133295943 6 28.875801438835826 39.4582068701908 15.930829401031122 15.735162289942256 7 26.10028622002994 36.96857040148161 19.48161866755248 17.44952471093597 8 27.622394328384015 34.25152779610577 18.5115875883346 19.614490287175613 9 27.60191753768867 16.240142700479158 19.994448514522595 36.16349124730958 10-11 25.300298165163703 28.652125095131865 25.202919538634628 20.8446572010698 12-13 27.271713724849988 26.474842733309025 27.069609175421398 19.183834366419585 14-15 23.29604661427642 26.772550634552992 28.097057712696976 21.834345038473614 16-17 19.801739162089724 30.28323951929596 28.532587674792158 21.382433643822154 18-19 19.79895204335619 26.72198433753032 34.15614007944995 19.322923539663545 20-21 21.317078553519504 25.036061903613472 34.85798208053294 18.78887746233408 22-23 20.74969114103763 25.47420942344052 34.88172351264217 18.894375922879682 24-25 20.407890470800368 25.836001979443367 35.079263055509735 18.67684449424653 26-27 20.099415214657345 25.938850371513027 35.13581001102907 18.825924402800553 28-29 19.66714049005739 26.46596577209038 35.04802794477256 18.818865793079674 30-31 19.90829774054779 26.011372627313534 35.00060577413669 19.079723858001994 32-33 19.805550120358024 26.219449765882025 34.90968597703869 19.065314136721256 34-35 20.443345270543908 26.037092568745134 34.70850150845691 18.81106065225404 36-37 20.540451069827604 25.757883426701866 34.65016236397857 19.05150313949196 38-39 19.955360240808975 26.339790020215304 34.736649310552345 18.968200428423376 40-41 20.091053566207798 26.29166036246236 34.36219843625397 19.25508763507587 42-43 20.115240692513623 26.62715329663016 33.704062720790766 19.553543290065452 44-45 20.506108649146427 26.510780788826 33.31080877388471 19.672301788142867 46-47 20.916416015580563 27.37286188177156 32.21260824259081 19.49811386005706 48-49 20.988369182885044 27.682914620883597 31.511619441120125 19.817096755111237 50-51 20.892412666487683 28.020440387511886 31.137462971136827 19.949683974863603 52-53 21.206845163609557 28.45090803190739 30.371972278975797 19.970274525507257 54-55 20.728313941054143 28.334816004659608 30.54556996009301 20.391300094193237 56-57 21.213784520456315 28.388908860079816 30.207134113878258 20.190172505585615 58-59 21.584186912145466 27.884326609361988 30.341370852881084 20.190115625611462 60-61 21.075679943211036 28.27372691241849 30.857841018197043 19.792752126173436 62-63 20.95685767720387 28.172708078321453 30.9530580949304 19.91737614954428 64-65 20.865451558738812 28.425027643667438 30.68964693462443 20.019873862969316 66-67 20.997868138568716 28.318093292258407 30.30104295120608 20.382995617966788 68-69 21.08614585845532 28.414732368345614 30.18034364605185 20.31877812714722 70-71 21.25951601967592 28.608465560313252 30.057312261957307 20.07470615805352 72-73 21.335735185041933 28.50625224675898 29.917330645564956 20.240681922634135 74-75 20.968461191931233 29.39022392508225 29.351431782709398 20.28988310027712 76-77 20.998550698258565 28.778479803058776 29.749136561992344 20.473832936690314 78-79 20.925459931471007 28.93580981156802 29.637026132935322 20.501704124025647 80-81 20.9586328776676 29.113690050606138 29.536422258966706 20.391254812759552 82-83 21.06801616761903 28.9036006284121 29.48622419204565 20.54215901192322 84-85 20.723232162358936 29.021489352020385 29.341781943938845 20.913496541681834 86-87 20.879671552277244 28.872673040257368 29.074369428606534 21.173285978858853 88-89 20.77387480035129 28.907085424620384 28.993599865308223 21.325439909720107 90-91 20.947218545268996 28.69716777582032 29.054630202161952 21.30098347674873 92-93 20.953995848779066 28.843590891431752 29.134199109575054 21.068214150214132 94-95 20.745889853067652 28.578432933960073 29.25211934783697 21.423557865135308 96-97 20.657327733310275 28.587647489772984 29.181360659989714 21.573664116927024 98-99 20.813804542207215 28.680361847643574 28.70015607864908 21.805677531500127 100-101 20.876054081850906 28.20001729308595 29.081054514178057 21.842874110885088 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1377.0 1 939.5 2 717.0 3 989.5 4 903.5 5 889.0 6 1670.0 7 3709.0 8 5531.0 9 5957.5 10 4964.5 11 3719.0 12 3474.0 13 3512.5 14 3661.0 15 3752.0 16 3749.0 17 3768.5 18 3849.5 19 4026.5 20 4210.5 21 4489.0 22 4967.5 23 5558.5 24 6206.0 25 7101.5 26 8260.5 27 9340.5 28 10460.0 29 11765.5 30 13020.0 31 14655.0 32 16900.0 33 18432.5 34 19678.5 35 20578.0 36 21816.5 37 23800.0 38 25041.0 39 25252.5 40 24923.5 41 25659.5 42 26618.5 43 27433.5 44 28262.5 45 28691.0 46 28576.0 47 29915.0 48 33540.5 49 32886.0 50 28517.5 51 25200.0 52 22670.5 53 21772.5 54 21594.5 55 20291.5 56 18361.0 57 16452.5 58 14861.0 59 13588.5 60 11832.5 61 9984.5 62 8469.0 63 6707.0 64 5106.0 65 3973.0 66 2865.0 67 2148.0 68 1620.5 69 1070.5 70 724.5 71 576.0 72 502.0 73 410.0 74 318.5 75 259.5 76 205.0 77 144.5 78 103.0 79 69.5 80 45.5 81 33.0 82 28.5 83 17.0 84 6.0 85 3.0 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051305736686673244 2 0.0 3 0.0 4 0.0 5 0.0017063992246121923 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.1375994830747949E-4 12-13 0.004948557751375358 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.2751989661495898E-4 24-25 0.0010238395347673154 26-27 3.9815981907617823E-4 28-29 0.0027871187335332475 30-31 3.9815981907617823E-4 32-33 0.0 34-35 0.0 36-37 5.6879974153739746E-5 38-39 7.963196381523565E-4 40-41 1.1375994830747949E-4 42-43 0.01148975477905543 44-45 3.9815981907617823E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.6879974153739746E-5 82-83 3.4127984492243843E-4 84-85 4.5503979322991797E-4 86-87 0.0 88-89 0.0 90-91 0.008020076355677305 92-93 0.002900878681840727 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.009100795864598358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 879044.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.819332056747385 #Duplication Level Percentage of deduplicated Percentage of total 1 78.64416127248484 42.325762298482545 2 11.899402964977472 12.808358388983338 3 3.8364223510078372 6.194210652564547 4 1.7177287473596383 3.697880553502765 5 0.9613094251025347 2.5868515579437124 6 0.6118669416494859 1.9758162064308096 7 0.3938224083933768 1.4836681278097787 8 0.29758283436929195 1.2812567501847183 9 0.21379459613811252 1.0355654125345752 >10 1.2839517796290136 12.704697701988781 >50 0.09316447124261405 3.4090736737541856 >100 0.04213415984041591 4.27363231788888 >500 0.0027524827941346223 1.0509735172797947 >1k 0.0012703766742159794 1.4209736268544986 >5k 2.1172944570266324E-4 0.9903513866072532 >10k+ 4.234588914053265E-4 2.7609278271898674 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13142 1.4950332406568954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11087 1.2612565468850252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8691 0.9886877107403041 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3584 0.4077156547340065 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2574 0.2928181069434522 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2381 0.27086243692010864 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1650 0.18770391470734116 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1240 0.14106233590127457 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1041 0.11842410618808615 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.10977835011671772 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 959 0.10909579042687283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.6879974153739746E-5 0.0 18-19 0.0 0.0 0.0 1.1375994830747949E-4 0.0 20-21 0.0 0.0 0.0 2.8439987076869875E-4 0.0 22-23 0.0 0.0 0.0 4.5503979322991797E-4 0.0 24-25 0.0 0.0 0.0 4.5503979322991797E-4 0.0 26-27 0.0 0.0 0.0 6.82559689844877E-4 0.0 28-29 0.0 0.0 0.0 0.0010807195089210552 0.0 30-31 0.0 0.0 0.0 0.002673358785225768 0.0 32-33 0.0 0.0 0.0 0.005744877389527715 0.0 34-35 0.0 0.0 0.0 0.009953995476904455 0.0 36-37 0.0 0.0 0.0 0.016779592375353224 0.0 38-39 0.0 0.0 0.0 0.030260146249789545 0.0 40-41 0.0 0.0 0.0 0.04595901911622172 0.0 42-43 0.0 0.0 0.0 0.06273861149157493 0.0 44-45 0.0 0.0 0.0 0.08463740154076474 0.0 46-47 0.0 0.0 0.0 0.11313426859178835 0.0 48-49 0.0 0.0 0.0 0.14601089365264994 0.0 50-51 0.0 0.0 0.0 0.1866231951984201 0.0 52-53 0.0 0.0 0.0 0.2289987759429562 0.0 54-55 0.0 0.0 0.0 0.2741045954468718 0.0 56-57 0.0 0.0 0.0 0.3371276068092155 0.0 58-59 0.0 0.0 0.0 0.4105596534416935 0.0 60-61 0.0 0.0 0.0 0.49218241635231 0.0 62-63 0.0 0.0 0.0 0.5805738961872215 0.0 64-65 0.0 0.0 0.0 0.6730607341612024 0.0 66-67 0.0 0.0 0.0 0.7791418859579271 0.0 68-69 0.0 0.0 0.0 0.9092832668216835 0.0 70-71 0.0 0.0 0.0 1.071618713056457 0.0 72-73 0.0 0.0 0.0 1.2327027998598479 0.0 74-75 0.0 0.0 0.0 1.401124403329071 0.0 76-77 0.0 0.0 0.0 1.6002043128671604 0.0 78-79 0.0 0.0 0.0 1.8296012486291926 0.0 80-81 0.0 0.0 0.0 2.0878363312871713 0.0 82-83 0.0 0.0 0.0 2.357959328543281 0.0 84-85 0.0 0.0 0.0 2.6247264073243204 0.0 86-87 0.0 0.0 0.0 2.9073061189200997 0.0 88-89 0.0 0.0 0.0 3.2170744581613664 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9695 0.0 30.285769 1 GTATCAA 13500 0.0 27.380648 1 TAAGGTG 330 0.0 25.907944 5 AGGTAAG 295 0.0 24.151472 2 GTAAGGT 335 0.0 24.103409 4 GAGTACT 9790 0.0 23.894497 12-13 AGTACTT 9850 0.0 23.869501 12-13 GTACTTT 10155 0.0 22.801796 14-15 GGTAAGG 365 0.0 22.122309 3 TATCAAC 16840 0.0 21.972008 2 ATCAACG 17145 0.0 21.52573 3 TCAACGC 17205 0.0 21.47827 4 GATATAG 310 1.0913936E-11 21.456764 1 CAACGCA 17340 0.0 21.283659 5 TACTTTT 11025 0.0 21.260963 14-15 TGGTACT 270 1.0004442E-9 21.110176 2 AGAGTAC 14710 0.0 21.101206 10-11 AACGCAG 17695 0.0 20.937191 6 ACTTTTT 11550 0.0 20.335678 16-17 GTACATG 7370 0.0 20.306776 1 >>END_MODULE