FastQCFastQC Report
Thu 26 May 2016
SRR937155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501638
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.46722312568009067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51710.34435729516701097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36300.24173602426150642No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24110.160558003992973No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21340.14211148092949166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86250.028.990771
GGTATCA60100.027.2883821
CCGTCTA700.00838498727.1477539
CGTATAG908.9080643E-426.4095271
GTACATG60950.025.9718971
TACATGG62900.024.4734572
ATCAACG102700.024.193753
TATCAAC103250.024.1124952
TCAACGC103150.024.0898114
CAACGCA104700.023.6440035
ACATGGG62400.023.6020133
AACGCAG106150.023.3603276
GAGTACT57650.022.45297412-13
GTACTTT61900.021.1394714-15
ACGCAGA117000.021.0337077
AGTACTT60600.020.9680412-13
TACACCG1604.9785245E-520.7877775
CGCAGAG118650.020.6611238
GTATAGG4200.020.3730641
CATGGGG25750.020.1117674
Produced by FastQC (version 0.11.5)