##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937155_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1501638 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.602596631145456 33.0 31.0 34.0 30.0 34.0 2 31.725780114781326 33.0 31.0 34.0 30.0 34.0 3 31.753565772842723 33.0 31.0 34.0 30.0 34.0 4 35.378893581542286 37.0 35.0 37.0 33.0 37.0 5 35.381423485553775 37.0 35.0 37.0 33.0 37.0 6 35.41308690909527 37.0 35.0 37.0 33.0 37.0 7 35.343638746488836 37.0 35.0 37.0 33.0 37.0 8 35.31919344076269 37.0 35.0 37.0 33.0 37.0 9 36.90104139612876 39.0 37.0 39.0 33.0 39.0 10-11 36.772485778862816 39.0 37.0 39.0 33.0 39.0 12-13 36.619282077304916 39.0 37.0 39.0 32.0 39.0 14-15 37.69679709756945 40.0 37.5 41.0 32.5 41.0 16-17 37.6079005059808 40.0 37.0 41.0 32.0 41.0 18-19 37.524136642786075 40.0 37.0 41.0 32.0 41.0 20-21 37.379518232756496 40.0 37.0 41.0 32.0 41.0 22-23 37.46120469780333 40.0 37.0 41.0 32.0 41.0 24-25 37.411528277787326 40.0 37.0 41.0 32.0 41.0 26-27 37.32536903035219 40.0 37.0 41.0 32.0 41.0 28-29 37.17307633397663 39.5 37.0 41.0 31.0 41.0 30-31 36.90474501843987 39.0 36.0 41.0 31.0 41.0 32-33 36.653190382768685 39.0 36.0 41.0 30.0 41.0 34-35 36.634658286484495 39.0 36.0 41.0 30.0 41.0 36-37 36.479076182142435 39.0 35.5 41.0 30.0 41.0 38-39 36.32296498889879 39.0 35.0 41.0 30.0 41.0 40-41 36.07922881546685 39.0 35.0 40.5 29.5 41.0 42-43 35.792824568904095 39.0 35.0 40.0 28.5 41.0 44-45 35.52587441180897 38.5 35.0 40.0 27.0 41.0 46-47 35.105633648056326 38.0 34.5 40.0 25.5 41.0 48-49 34.7509269877294 38.0 34.0 40.0 24.0 41.0 50-51 33.772484446983896 37.5 33.0 39.5 23.5 40.5 52-53 33.891308024970066 38.0 33.5 39.5 23.0 40.0 54-55 34.159543445224486 38.0 34.0 40.0 23.0 41.0 56-57 33.85385725454471 38.0 33.0 40.0 21.5 41.0 58-59 33.552737743717195 38.0 33.0 40.0 20.0 41.0 60-61 33.17733601573748 37.0 32.5 40.0 18.5 41.0 62-63 32.76500794465777 36.5 32.0 39.5 15.5 41.0 64-65 32.292541877602986 36.0 31.0 39.0 12.0 41.0 66-67 31.81700782745242 35.5 31.0 39.0 8.0 40.0 68-69 31.284032836142934 35.0 30.5 38.5 7.0 40.0 70-71 30.77021326045292 35.0 30.0 38.0 2.0 40.0 72-73 30.231548482390565 34.5 29.0 37.0 2.0 39.0 74-75 29.64619036012674 34.0 29.0 36.5 2.0 39.0 76-77 29.085056784657823 34.0 28.0 36.0 2.0 39.0 78-79 28.490232665928804 34.0 26.5 35.5 2.0 37.5 80-81 27.846104720312084 33.5 26.0 35.0 2.0 37.0 82-83 27.226495000792468 33.0 25.5 35.0 2.0 36.5 84-85 26.575524860186007 32.5 24.5 35.0 2.0 36.0 86-87 25.9166253784201 32.0 22.5 35.0 2.0 36.0 88-89 25.285605119209823 31.5 20.0 34.5 2.0 35.0 90-91 24.645139840627365 31.0 17.5 34.0 2.0 35.0 92-93 23.891337992245802 31.0 8.5 34.0 2.0 35.0 94-95 23.020272529064925 30.5 2.0 34.0 2.0 35.0 96-97 22.0602129141644 30.0 2.0 34.0 2.0 35.0 98-99 20.804981959700008 29.0 2.0 34.0 2.0 35.0 100-101 17.78618814920773 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10354842230746186 1101 2 0.0967584434440738 1101 3 0.09247932962567518 1101 4 0.14619559560972561 1101 5 0.04348112328851528 1101 6 0.16838734016184276 1101 7 0.05864059455535653 1101 8 0.05614945787037584 1101 9 0.15669842368100007 1101 10-11 0.2669903558905986 1101 12-13 0.2946355035183643 1101 14-15 0.4222318818267894 1101 16-17 0.4374252854710434 1101 18-19 0.4053899746380907 1101 20-21 0.5684917562780711 1101 22-23 0.4910407621716928 1101 24-25 0.42484651992994316 1101 26-27 0.4100064486002779 1101 28-29 0.3570544553820554 1101 30-31 0.3786292478268507 1101 32-33 0.38450090539191706 1101 34-35 0.47793074557561255 1101 36-37 0.36729357076217894 1101 38-39 0.5828298417158138 1101 40-41 0.660636836205228 1101 42-43 0.7500547741948935 1101 44-45 0.7526097357164119 1101 46-47 0.9365827718898103 1101 48-49 0.7937558095771209 1101 50-51 0.7688824133019239 1101 52-53 0.6938381291644973 1101 54-55 0.864612304439369 1101 56-57 0.9672350927998465 1101 58-59 0.9421232124857255 1101 60-61 0.8165222577459517 1101 62-63 0.988059096114597 1101 64-65 0.8825270609117126 1101 66-67 0.8688998782594552 1101 68-69 0.9169286074101954 1101 70-71 0.9167746785354396 1101 72-73 0.9735877366306624 1101 74-75 1.1260435740480226 1101 76-77 1.1159633194896195 1101 78-79 1.2390109680018817 1101 80-81 1.3754640728129424 1101 82-83 1.4031616459266019 1101 84-85 1.5800022978986235 1101 86-87 1.8458135902385315 1101 88-89 1.7657528551944566 1101 90-91 1.7227165714460746 1101 92-93 1.9124181936686426 1101 94-95 1.9712729763511874 1101 96-97 1.7460405067532498 1101 98-99 1.964544235357348 1101 100-101 2.1819248047253588 1102 1 0.08619591858906972 1102 2 0.11460673298299184 1102 3 0.01662409908526996 1102 4 0.025719641222025302 1102 5 -0.006097458764038777 1102 6 -0.045792134305187915 1102 7 -0.10199401129492003 1102 8 -0.13125758228827067 1102 9 0.03572668347276675 1102 10-11 0.03025262658965744 1102 12-13 0.07054031264082283 1102 14-15 0.2571352035917869 1102 16-17 0.24282935091476787 1102 18-19 0.2559105497496432 1102 20-21 0.24350415092358446 1102 22-23 0.23695052915235237 1102 24-25 0.2659471644515179 1102 26-27 0.2781274203404891 1102 28-29 0.2965685852779387 1102 30-31 0.34937788441584416 1102 32-33 0.34335068228830323 1102 34-35 0.3847230113168152 1102 36-37 0.604527085883646 1102 38-39 0.4192205209423818 1102 40-41 0.5101658396757216 1102 42-43 0.6637880414234516 1102 44-45 0.832927038641543 1102 46-47 0.6487791030747374 1102 48-49 0.8220156013470827 1102 50-51 0.6484064589142093 1102 52-53 0.6745024821640015 1102 54-55 0.8018953981428893 1102 56-57 0.7423962231915127 1102 58-59 0.9007250964718452 1102 60-61 0.8088375775278038 1102 62-63 0.8601463544190082 1102 64-65 0.8331963717694251 1102 66-67 1.0667184206491456 1102 68-69 1.1368096188132704 1102 70-71 1.156735015669799 1102 72-73 1.1934687480337374 1102 74-75 1.042751556199736 1102 76-77 1.2731274245962148 1102 78-79 1.2880937642339099 1102 80-81 1.4272736910578594 1102 82-83 1.2794630837054832 1102 84-85 1.2165912914534047 1102 86-87 1.515868126678786 1102 88-89 1.463323504673884 1102 90-91 1.38384696311687 1102 92-93 1.559666582364727 1102 94-95 1.5188684151216414 1102 96-97 1.3908099663467048 1102 98-99 1.6680643146830825 1102 100-101 1.7101845964953135 1103 1 0.20302115371752905 1103 2 0.09563102993971384 1103 3 0.07404230983344107 1103 4 0.02937603083127982 1103 5 0.11934005188327745 1103 6 0.07510342598332898 1103 7 -0.04161217978665377 1103 8 -0.0496442345801924 1103 9 0.05791574921357778 1103 10-11 0.10623235829334732 1103 12-13 0.06961579905207316 1103 14-15 0.05080583564104302 1103 16-17 0.0916366174436618 1103 18-19 0.1002876704297293 1103 20-21 0.025775900229028537 1103 22-23 -0.07730125460416559 1103 24-25 0.17567852052557953 1103 26-27 0.03353428397867475 1103 28-29 -0.051095119575904846 1103 30-31 0.0022968696598653082 1103 32-33 0.06632671864954176 1103 34-35 0.15216580919570788 1103 36-37 0.2520748937140027 1103 38-39 0.2288668710736772 1103 40-41 0.3019523130311228 1103 42-43 0.06701390354469794 1103 44-45 0.1861142807484839 1103 46-47 0.22211583533091783 1103 48-49 0.2985494741690147 1103 50-51 0.25458758419270566 1103 52-53 0.13917225700426883 1103 54-55 0.30672986460116647 1103 56-57 0.3458443630331871 1103 58-59 0.3488654312246595 1103 60-61 0.3747267981184663 1103 62-63 0.4996083664021853 1103 64-65 0.49870636921283307 1103 66-67 0.7066654940952191 1103 68-69 0.6520703797200049 1103 70-71 0.7570162650303409 1103 72-73 0.7954919042639617 1103 74-75 0.6532396821170074 1103 76-77 0.4743484235294524 1103 78-79 0.5074066312195811 1103 80-81 0.5461042577945499 1103 82-83 0.6551129243070086 1103 84-85 0.8755596772797958 1103 86-87 0.9598624494257422 1103 88-89 0.9231092591275107 1103 90-91 0.8459624918141451 1103 92-93 1.1416408601778585 1103 94-95 1.1051425440017901 1103 96-97 0.9406862417748343 1103 98-99 1.0738150850494357 1103 100-101 1.1733724376870533 1104 1 0.0022835354393038187 1104 2 0.18743204874893138 1104 3 0.12077912862731566 1104 4 0.08299125943351982 1104 5 0.20083437650947644 1104 6 0.21000777484294275 1104 7 0.1830114301950161 1104 8 0.17571740002498615 1104 9 0.20798668186235147 1104 10-11 0.2757343415123046 1104 12-13 0.16845548009133893 1104 14-15 0.43399312170659954 1104 16-17 0.32183287205757694 1104 18-19 0.3052807397029653 1104 20-21 0.21688351620631607 1104 22-23 0.31265193441903705 1104 24-25 0.24093998593873778 1104 26-27 0.21945947378002018 1104 28-29 0.2825278194281111 1104 30-31 0.36109772440717336 1104 32-33 0.3753036732846482 1104 34-35 0.5102610199593869 1104 36-37 0.55557405994427 1104 38-39 0.39664049002033863 1104 40-41 0.6073460641517414 1104 42-43 0.5085144046192269 1104 44-45 0.5008546083338885 1104 46-47 0.8094728335363968 1104 48-49 0.7853087130639764 1104 50-51 0.6856710429428219 1104 52-53 0.6300782495305626 1104 54-55 0.7849652492501562 1104 56-57 0.7236711928948978 1104 58-59 0.9572796230369036 1104 60-61 0.8903569964809961 1104 62-63 0.8724061654329489 1104 64-65 0.9199426849004553 1104 66-67 1.1711685668274612 1104 68-69 1.010675299850302 1104 70-71 0.9336987503803265 1104 72-73 0.9985373097940169 1104 74-75 0.8807499943984354 1104 76-77 1.0911960577618025 1104 78-79 1.2067396916243318 1104 80-81 1.3465899927893616 1104 82-83 1.4367220160795888 1104 84-85 1.4771411731282207 1104 86-87 1.3958036390726711 1104 88-89 1.4455434553181234 1104 90-91 1.6556843281494373 1104 92-93 1.5237597121102056 1104 94-95 1.3725745027536824 1104 96-97 1.225698475307567 1104 98-99 1.4113332245569161 1104 100-101 1.588825571091732 1105 1 0.18196663536295077 1105 2 0.05652866986327609 1105 3 0.09876652487709592 1105 4 0.1885869643329059 1105 5 0.1726091480152192 1105 6 0.1880886841629703 1105 7 0.19367511723154252 1105 8 0.1429942194936089 1105 9 0.1738088535715292 1105 10-11 0.1801851640276908 1105 12-13 0.1214080154145023 1105 14-15 0.2487161551746908 1105 16-17 0.20084893872201093 1105 18-19 0.3087047567367307 1105 20-21 0.30000291507979426 1105 22-23 0.30363165300596506 1105 24-25 0.08501999582821895 1105 26-27 0.18495408332432106 1105 28-29 0.23656414725775932 1105 30-31 0.20140430873971127 1105 32-33 0.3639106642258696 1105 34-35 0.39052605891251346 1105 36-37 0.26214003621273463 1105 38-39 0.24732702515061789 1105 40-41 0.27214286904191454 1105 42-43 0.23253442227748167 1105 44-45 0.11296132726130281 1105 46-47 0.29032117025591475 1105 48-49 0.38269738055867464 1105 50-51 0.2604760375531612 1105 52-53 0.07600584856782433 1105 54-55 0.4297176207789093 1105 56-57 0.493606527674352 1105 58-59 0.3349123090253414 1105 60-61 0.5186551971403333 1105 62-63 0.413685791892199 1105 64-65 0.600372557291081 1105 66-67 0.7822771469580445 1105 68-69 0.49752419178702567 1105 70-71 0.48776955282903245 1105 72-73 0.5352229401653545 1105 74-75 0.4392184421294232 1105 76-77 0.3584641386871077 1105 78-79 0.598371763783387 1105 80-81 0.5919256620845168 1105 82-83 0.823036631346568 1105 84-85 0.8140128172478462 1105 86-87 0.793233654796726 1105 88-89 0.9205409643004394 1105 90-91 0.9934307590672553 1105 92-93 1.2249445573447417 1105 94-95 1.1509086966203945 1105 96-97 1.2525834830044076 1105 98-99 1.4707490582645697 1105 100-101 1.1631558070377288 1106 1 -0.006721691138736219 1106 2 -0.6163201934425544 1106 3 -0.1807233261131458 1106 4 0.0775768538331647 1106 5 -0.009269254583934128 1106 6 -0.07803269205217589 1106 7 -0.06090143770531142 1106 8 -0.08448398426412496 1106 9 -0.05328255734212206 1106 10-11 -0.6315825639071306 1106 12-13 -0.49682448517160793 1106 14-15 -0.3012715300859625 1106 16-17 -0.3027955470254753 1106 18-19 -0.04928852474774459 1106 20-21 -0.15608608169354454 1106 22-23 -0.18997723707913394 1106 24-25 -0.14743671990838436 1106 26-27 -0.21568021744251809 1106 28-29 -0.07971475323491717 1106 30-31 -0.06297353736864864 1106 32-33 0.009837786360151313 1106 34-35 -0.08165391514894793 1106 36-37 -0.07469305294174688 1106 38-39 0.08907811055453152 1106 40-41 0.18183308775337537 1106 42-43 -0.17107304872082096 1106 44-45 -0.03523562844485184 1106 46-47 0.03814888126890281 1106 48-49 0.3507713130061987 1106 50-51 0.10181197658941699 1106 52-53 0.18944335923566058 1106 54-55 0.29309017225466505 1106 56-57 -0.13107167393923191 1106 58-59 -0.08202992285682598 1106 60-61 -0.007771274273530082 1106 62-63 0.09964174151941307 1106 64-65 0.246683086774091 1106 66-67 0.42239165569586135 1106 68-69 0.5274547919535344 1106 70-71 0.5029419323780466 1106 72-73 0.6395043786510435 1106 74-75 0.4174759445987206 1106 76-77 0.6602837295894943 1106 78-79 0.7286394182247449 1106 80-81 0.5757926482290934 1106 82-83 0.4504156118779967 1106 84-85 0.5774305932990202 1106 86-87 0.515561109499501 1106 88-89 0.7332619913017417 1106 90-91 0.5681634530497917 1106 92-93 0.8578318798777822 1106 94-95 0.7238027845016468 1106 96-97 0.7114878012883707 1106 98-99 0.44633060878194186 1106 100-101 0.2083895515112637 1107 1 0.16817664751810568 1107 2 0.19313066710278193 1107 3 0.026871393678778333 1107 4 0.007290430923703184 1107 5 -0.010292265427331415 1107 6 -0.049473495578595816 1107 7 -0.040662326890924305 1107 8 0.07261661093956917 1107 9 0.09178026524197946 1107 10-11 0.0760751236685806 1107 12-13 -0.08663235867479102 1107 14-15 0.015662864671440957 1107 16-17 0.05289422479250305 1107 18-19 0.1455760643270949 1107 20-21 0.03388110892833396 1107 22-23 0.008915277488995343 1107 24-25 0.0633853144455685 1107 26-27 -0.0035706500484522508 1107 28-29 0.022544783868490015 1107 30-31 0.08805684846860373 1107 32-33 0.010465164004976657 1107 34-35 0.17238342675950236 1107 36-37 0.13932553089266975 1107 38-39 0.21543159444124882 1107 40-41 0.2081485897029367 1107 42-43 -0.008933880717883369 1107 44-45 -7.343146033491621E-4 1107 46-47 0.38201522970386037 1107 48-49 0.24343223091546662 1107 50-51 0.3105642685161172 1107 52-53 0.26827571800597383 1107 54-55 0.3672159048301964 1107 56-57 0.5750785246393093 1107 58-59 0.2673376246975039 1107 60-61 0.14814630291016329 1107 62-63 0.23222702333186618 1107 64-65 0.13182496124162668 1107 66-67 0.28619075798803806 1107 68-69 0.19748275942535187 1107 70-71 0.2684238846323659 1107 72-73 0.36898931655589706 1107 74-75 0.28075089040761014 1107 76-77 0.4919062943679613 1107 78-79 0.3947030030353069 1107 80-81 0.31905861753474696 1107 82-83 0.30291947260820606 1107 84-85 0.3478351023673909 1107 86-87 0.4725109845558926 1107 88-89 0.6622925548289054 1107 90-91 0.736828412398193 1107 92-93 0.7252378285794521 1107 94-95 0.3246126249573855 1107 96-97 0.3173527471806423 1107 98-99 0.6146541332296884 1107 100-101 0.43648459778683346 1108 1 -0.07554589648144727 1108 2 -0.06766006571011118 1108 3 -0.01170271303937298 1108 4 -0.09110068242586067 1108 5 -0.15143219659496765 1108 6 -0.00593562378588075 1108 7 -0.06407092813886095 1108 8 -0.17129620139429136 1108 9 -0.20103150901023525 1108 10-11 -0.10358015487867078 1108 12-13 -0.04701737224539926 1108 14-15 -0.06537745542705409 1108 16-17 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2.313833845739726 2315 88-89 2.2208861872805308 2315 90-91 2.2316161607857445 2315 92-93 2.506790946651936 2315 94-95 2.8062725709917125 2315 96-97 3.009636809251198 2315 98-99 3.329591490917789 2315 100-101 3.6482359243594082 2316 1 -0.31816622196621225 2316 2 -0.2560992970120495 2316 3 -0.4983587033440813 2316 4 -0.2539469428136769 2316 5 -0.07096561072238927 2316 6 -0.28991242433989584 2316 7 -0.055596205147175226 2316 8 -0.007727122446922863 2316 9 -0.2153710173204857 2316 10-11 -0.1564541160975068 2316 12-13 -0.2547497995307211 2316 14-15 -0.2777216382525438 2316 16-17 -0.2761561473499725 2316 18-19 0.009783101807151695 2316 20-21 -0.1286396579509841 2316 22-23 0.022267134878681816 2316 24-25 -0.2480227630398133 2316 26-27 -0.12183345905960863 2316 28-29 0.08591616212473951 2316 30-31 0.16400008012729472 2316 32-33 0.33343457854511627 2316 34-35 -0.10136516109270133 2316 36-37 -0.03269442192497962 2316 38-39 0.09051258527059503 2316 40-41 -0.06427756327420298 2316 42-43 0.13466992045171366 2316 44-45 0.11545097580069097 2316 46-47 0.17994892521156913 2316 48-49 -0.01969160613928267 2316 50-51 0.061595136405536266 2316 52-53 0.1615891982026767 2316 54-55 0.3401371402103095 2316 56-57 -0.054711563744525904 2316 58-59 0.34563748182137743 2316 60-61 0.2846025595555588 2316 62-63 0.46582658805807853 2316 64-65 0.3553351453192519 2316 66-67 0.5960201373075478 2316 68-69 0.713317504906481 2316 70-71 0.9489279383272127 2316 72-73 1.104697134870623 2316 74-75 0.9852938529275441 2316 76-77 1.037066365473759 2316 78-79 1.2267832628705015 2316 80-81 1.6010516564767947 2316 82-83 1.6944706754258583 2316 84-85 1.7057393919739816 2316 86-87 1.7115834526579654 2316 88-89 1.7264335536327344 2316 90-91 1.665313094748008 2316 92-93 1.9825378019978466 2316 94-95 2.4159374687904567 2316 96-97 2.853111652018498 2316 98-99 3.2773165302259635 2316 100-101 3.672351490397144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7445.0 3 1637.0 4 1540.0 5 1773.0 6 1797.0 7 2295.0 8 2770.0 9 3524.0 10 4366.0 11 5440.0 12 6843.0 13 8376.0 14 9536.0 15 11586.0 16 14047.0 17 17603.0 18 16124.0 19 12453.0 20 13096.0 21 13900.0 22 15586.0 23 17407.0 24 19486.0 25 22344.0 26 25556.0 27 29628.0 28 34467.0 29 41025.0 30 48736.0 31 58877.0 32 72170.0 33 91707.0 34 117714.0 35 154991.0 36 202655.0 37 235983.0 38 144241.0 39 12914.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55374302347719 18.96859353968143 14.804824363637579 22.672839073203807 2 15.077162637151313 20.2832368243929 40.34605666606126 24.293543872394526 3 19.052636499001757 24.611820008947472 29.92728511047455 26.40825838157622 4 12.394667329043907 16.65117558605696 37.83529509173123 33.1188619931679 5 13.567743232153672 37.288708248282454 34.35501220926622 14.788536310297651 6 31.432976891345266 36.33699120242434 17.71863777751078 14.511394128719612 7 28.462130991937997 32.72101768995913 20.733670729932758 18.083180588170116 8 26.393400164742225 34.37952657302794 19.922194676182734 19.30487858604711 9 26.09351050250746 16.936261288927103 20.688244027750333 36.281984180815094 10-11 24.695614437721677 26.788933861881908 27.517329438764865 20.998122261631547 12-13 25.864078603214786 24.09079317522434 28.075428217421898 21.96970000413898 14-15 22.624792907386716 25.376938593786086 26.687312515269117 25.31095598355808 16-17 21.862617329272872 28.974043636060237 27.065137040590713 22.098201994076174 18-19 21.655272199190062 27.275411976070284 29.75474309724851 21.314572727491143 20-21 22.459911786073388 26.219098512003185 29.86835988639045 21.45262981553298 22-23 22.06312043578836 26.17259610956644 29.894209733158384 21.870073721486815 24-25 22.120917846344835 26.393622062484567 29.821696734277076 21.663763356893526 26-27 21.845563767464025 26.70702618719325 29.758550314142205 21.688859731200523 28-29 21.4894979726196 26.80487585903757 30.037904287086008 21.667721881256817 30-31 21.64640466086773 26.473903620257044 29.94875889442583 21.930932824449396 32-33 21.527033287451218 26.611770564510206 29.859543323885447 22.00165282415313 34-35 22.196522454254414 26.55516972527282 29.475931666653317 21.772376153819454 36-37 21.944768897046554 26.39409310862673 29.6846988152845 21.976439179042213 38-39 21.720939373686367 26.699762724091595 29.83396692552019 21.745330976701847 40-41 21.930117538481866 26.580573435108594 29.744639832155567 21.74466919425397 42-43 21.805882498202976 26.65171203002852 29.344948204943037 22.197457266825467 44-45 21.879396508688234 26.898891312298613 29.109292367327217 22.11241981168594 46-47 21.973118564863903 27.024976780028187 28.790862728332506 22.21104192677541 48-49 22.155763169172317 27.12485030859459 28.566960954073274 22.15242556815982 50-51 21.871652559501616 27.260937879181867 28.622496001847946 22.244913559468568 52-53 22.27838229608941 27.34534149961641 28.24401767239566 22.132258531898515 54-55 21.841187345157877 27.185428728484812 28.43631443459826 22.537069491759055 56-57 22.037283088674464 27.610860598165743 28.295287027329675 22.056569285830115 58-59 22.37052117155169 27.130500406362472 28.284663252954967 22.214315169130867 60-61 22.19352901433826 27.133698671270928 28.849018675608274 21.823753638782538 62-63 21.99251795603799 27.20232148898814 28.79492512951103 22.01023542546284 64-65 21.875573432373514 27.186216137045914 28.601488939052523 22.336721491528046 66-67 21.84167985973365 27.50221460017917 28.249223013157476 22.406882526929707 68-69 21.746183639095978 27.294821387102715 28.640157005314475 22.31883796848683 70-71 21.88490983055191 27.25627155471561 28.507512786017063 22.35130582871542 72-73 21.997652348752332 27.073196936441914 28.646750558384642 22.282400156421104 74-75 21.733542038371073 27.520902481336506 28.294203363283238 22.451352117009183 76-77 21.9070661496259 27.295280556908967 28.293832219826864 22.503821073638264 78-79 21.916411620558137 27.363235382946264 28.2791654444143 22.4411875520813 80-81 21.79047560571923 27.680988318081347 28.112252933827374 22.41628314237205 82-83 21.967928798582857 27.470896914567444 28.170158414077456 22.391015872772243 84-85 21.864210509108002 27.429031443385128 27.94801127236466 22.758746775142214 86-87 21.986548645639655 27.260461765998212 28.28988172430095 22.463107864061183 88-89 21.91978736133258 27.138457041670545 28.09741312088152 22.844342476115354 90-91 21.867452874572656 27.134049293260396 28.30622068320275 22.692277148964198 92-93 22.076148814022513 26.940550136057485 28.32741887853929 22.65588217138071 94-95 21.86988557706456 26.872189517720656 28.23480108200127 23.023123823213513 96-97 21.935882792038022 26.773220572792315 28.34523438889506 22.9456622462746 98-99 21.870599243687273 26.980115088745944 27.92697430859321 23.22231135897357 100-101 22.243249149627236 26.049449238709936 28.708050640344453 22.999250971318375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2261.0 1 1306.5 2 393.5 3 444.0 4 380.0 5 353.0 6 582.0 7 1248.0 8 2074.0 9 2483.0 10 2178.5 11 1798.5 12 1888.0 13 2030.0 14 2149.0 15 2268.0 16 2323.0 17 2399.5 18 2534.0 19 2705.0 20 2956.0 21 3396.0 22 4009.0 23 4747.5 24 5869.5 25 7234.0 26 9102.5 27 11274.0 28 12922.0 29 14698.0 30 16987.5 31 19673.5 32 22963.5 33 26324.5 34 30099.0 35 33139.5 36 36086.5 37 39474.5 38 41787.5 39 42865.5 40 43745.0 41 46445.5 42 49695.5 43 52676.0 44 54512.5 45 55691.5 46 55996.0 47 57705.5 48 63816.0 49 64271.0 50 59715.5 51 56873.0 52 52864.0 53 50258.0 54 48340.5 55 44043.5 56 39083.5 57 34224.5 58 29756.0 59 25865.5 60 22065.5 61 18000.0 62 14253.0 63 10988.5 64 8208.0 65 6304.5 66 4775.5 67 3649.0 68 2613.5 69 1694.5 70 1268.0 71 1007.5 72 797.0 73 624.5 74 463.5 75 342.5 76 250.0 77 161.0 78 99.0 79 67.5 80 39.0 81 29.0 82 20.5 83 11.5 84 11.5 85 10.0 86 4.5 87 3.5 88 3.0 89 3.0 90 3.0 91 3.5 92 2.5 93 1.0 94 0.5 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3098616310988401 2 0.26784085112390604 3 0.26690853587882035 4 0.26650897220235503 5 0.26670875404058764 6 0.23294562337926986 7 0.23307881127142493 8 0.23574256911452693 9 0.24706353994771044 10-11 0.2547551407196675 12-13 0.24533209734969413 14-15 0.23414431440866573 16-17 0.22978241094058618 18-19 0.2164303247520374 20-21 0.22298982844067614 22-23 0.21949364627160475 24-25 0.22295653146763733 26-27 0.21789539156574353 28-29 0.23457717505816983 30-31 0.24769618243544717 32-33 0.23947183009486975 34-35 0.23774038749685344 36-37 0.22562028931073932 38-39 0.21120269998494978 40-41 0.20694068743598656 42-43 0.2216912464921639 44-45 0.21909408259513943 46-47 0.230847914077827 48-49 0.23680807225176775 50-51 0.2502933463324716 52-53 0.2620471778151592 54-55 0.23570927214148818 56-57 0.2102370877668253 58-59 0.19818358352678872 60-61 0.20147998385762747 62-63 0.2075733299237233 64-65 0.19891611693364178 66-67 0.2038107719703417 68-69 0.20577529337962946 70-71 0.20817267543842125 72-73 0.2077065178158784 74-75 0.21253457890650077 76-77 0.22428841038918834 78-79 0.22505424076908015 80-81 0.21669670053634763 82-83 0.20833916030361513 84-85 0.19502037108810513 86-87 0.1983500683919826 88-89 0.20867213003400287 90-91 0.2089052088452743 92-93 0.2124679849604232 94-95 0.2076732208428396 96-97 0.21586427621037826 98-99 0.22082552519315574 100-101 0.20181295358801524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1501638.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.64763358838331 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64496642449902 46.48991475186995 2 11.200282156146436 12.91339523648075 3 3.3930743645717425 5.86808123120905 4 1.4841666010297396 3.4223476960111485 5 0.7960155576704365 2.294420659961896 6 0.4979380098868703 1.7222968766212252 7 0.3249671380327744 1.3113510551105272 8 0.24263025863505056 1.1189648197798454 9 0.1819520621410608 0.9440195228062762 >10 1.071491221429962 11.690235935561134 >50 0.10474338260470964 4.157142589133374 >100 0.054299592562975035 5.497760318673181 >500 0.0025470359120025547 0.9525968426221718 >1k 6.946461578188785E-4 0.8040977201647816 >5k 2.315487192729595E-4 0.8133747439947039 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7016 0.46722312568009067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5171 0.34435729516701097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3630 0.24173602426150642 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2411 0.160558003992973 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2134 0.14211148092949166 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.329697303877499E-5 0.0 16-17 0.0 0.0 0.0 6.659394607754998E-5 0.0 18-19 0.0 0.0 0.0 6.659394607754998E-5 0.0 20-21 0.0 0.0 0.0 6.659394607754998E-5 0.0 22-23 0.0 0.0 0.0 1.9978183823264993E-4 0.0 24-25 0.0 0.0 0.0 2.663757843101999E-4 0.0 26-27 0.0 0.0 0.0 3.662667034265249E-4 0.0 28-29 0.0 0.0 0.0 7.658303798918248E-4 0.0 30-31 0.0 0.0 0.0 0.0016648486519387495 0.0 32-33 0.0 0.0 0.0 0.0027969457352570995 0.0 34-35 0.0 0.0 0.0 0.003496182169071374 0.0 36-37 0.0 0.0 0.0 0.005360812659242773 0.0 38-39 0.0 0.0 0.0 0.008390837205771298 0.0 40-41 0.0 0.0 0.0 0.012919225539044697 0.0 42-43 0.0 0.0 0.0 0.017048050195852794 0.0 44-45 0.0 0.0 0.0 0.01921235344337317 0.0 46-47 0.0 0.0 0.0 0.022875020477638418 3.329697303877499E-5 48-49 0.0 0.0 0.0 0.02863539681334649 6.659394607754998E-5 50-51 0.0 0.0 0.0 0.03646018547745862 6.659394607754998E-5 52-53 0.0 0.0 0.0 0.05407428421497058 6.659394607754998E-5 54-55 0.0 0.0 0.0 0.067626152241752 6.659394607754998E-5 56-57 0.0 0.0 0.0 0.09789310073399848 6.659394607754998E-5 58-59 0.0 0.0 0.0 0.15289970019405474 6.659394607754998E-5 60-61 0.0 0.0 0.0 0.20527583878404781 6.659394607754998E-5 62-63 0.0 0.0 0.0 0.23800676328116363 6.659394607754998E-5 64-65 0.0 0.0 0.0 0.2958769024225546 6.659394607754998E-5 66-67 0.0 0.0 0.0 0.3902072270414041 6.659394607754998E-5 68-69 0.0 0.0 0.0 0.5231953373582714 6.659394607754998E-5 70-71 0.0 0.0 0.0 0.6556173991334795 6.659394607754998E-5 72-73 0.0 0.0 0.0 0.8002261530408794 6.659394607754998E-5 74-75 0.0 0.0 0.0 0.9717388611636093 6.659394607754998E-5 76-77 0.0 0.0 0.0 1.1131844026323257 9.989091911632497E-5 78-79 0.0 0.0 0.0 1.2988816212695737 1.3318789215509996E-4 80-81 0.0 0.0 0.0 1.5368883845507373 1.3318789215509996E-4 82-83 0.0 0.0 0.0 1.7561822489841092 1.3318789215509996E-4 84-85 0.0 0.0 0.0 2.014067305169422 1.3318789215509996E-4 86-87 0.0 0.0 0.0 2.33022206417259 1.3318789215509996E-4 88-89 0.0 0.0 0.0 2.7232262369492513 1.3318789215509996E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8625 0.0 28.99077 1 GGTATCA 6010 0.0 27.288382 1 CCGTCTA 70 0.008384987 27.147753 9 CGTATAG 90 8.9080643E-4 26.409527 1 GTACATG 6095 0.0 25.971897 1 TACATGG 6290 0.0 24.473457 2 ATCAACG 10270 0.0 24.19375 3 TATCAAC 10325 0.0 24.112495 2 TCAACGC 10315 0.0 24.089811 4 CAACGCA 10470 0.0 23.644003 5 ACATGGG 6240 0.0 23.602013 3 AACGCAG 10615 0.0 23.360327 6 GAGTACT 5765 0.0 22.452974 12-13 GTACTTT 6190 0.0 21.13947 14-15 ACGCAGA 11700 0.0 21.033707 7 AGTACTT 6060 0.0 20.96804 12-13 TACACCG 160 4.9785245E-5 20.787777 5 CGCAGAG 11865 0.0 20.661123 8 GTATAGG 420 0.0 20.373064 1 CATGGGG 2575 0.0 20.111767 4 >>END_MODULE