FastQCFastQC Report
Thu 26 May 2016
SRR937155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501638
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81440.5423410968555671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64550.4298639219305851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46440.30926228558414215No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28820.19192375259549907No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.11334289622399008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100600.027.7762621
GGTATCA70250.025.9087751
CGATCGT2150.024.30223324-25
ATTCGCG2000.023.74911780-81
CGCGATC2250.023.22213222-23
CCCGCGA2250.023.22174520-21
GTACATG63000.023.0066681
GTCTAGC2903.6379788E-1222.941641
ATCAACG120550.022.9315413
TCAACGC121100.022.8273934
TATCAAC122000.022.6589932
TACATGG62900.022.6541652
CAACGCA122750.022.4818535
GAGTACT69050.022.0502812-13
AACGCAG125500.022.027076
AGTACTT70150.021.97540312-13
CGTTAGG1302.9329045E-421.9332161
TAACGCG652.9437782E-421.9222676-77
ACATGGG60950.021.8982853
TCGCGGA2200.021.59010782-83
Produced by FastQC (version 0.11.5)