##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937155_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1501638 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23532169537532 33.0 31.0 34.0 31.0 34.0 2 32.457216053403016 34.0 31.0 34.0 31.0 34.0 3 32.52169897138991 34.0 31.0 34.0 31.0 34.0 4 36.142664876621396 37.0 35.0 37.0 35.0 37.0 5 36.07150458366131 37.0 35.0 37.0 35.0 37.0 6 36.112473179288216 37.0 35.0 37.0 35.0 37.0 7 36.05333176171621 37.0 35.0 37.0 35.0 37.0 8 36.026954565614346 37.0 35.0 37.0 35.0 37.0 9 37.72787183062762 39.0 37.0 39.0 35.0 39.0 10-11 37.64557769582282 39.0 37.0 39.0 35.0 39.0 12-13 37.536411238927094 39.0 37.0 39.0 35.0 39.0 14-15 38.76623760187209 40.0 38.0 41.0 35.0 41.0 16-17 38.72037168745064 40.0 38.0 41.0 35.0 41.0 18-19 38.70340687968738 40.0 38.0 41.0 34.5 41.0 20-21 38.59891698265494 40.0 38.0 41.0 34.0 41.0 22-23 38.54280891932676 40.0 38.0 41.0 34.0 41.0 24-25 38.576379593483914 40.0 38.0 41.0 34.0 41.0 26-27 38.42206843460275 40.0 38.0 41.0 34.0 41.0 28-29 38.27292563187666 40.0 38.0 41.0 34.0 41.0 30-31 38.31473364419388 40.0 38.0 41.0 34.0 41.0 32-33 38.21349819330624 40.0 38.0 41.0 34.0 41.0 34-35 38.069549718374205 40.0 37.0 41.0 33.5 41.0 36-37 37.900802323862344 40.0 37.0 41.0 33.0 41.0 38-39 37.78948321765965 40.0 37.0 41.0 33.0 41.0 40-41 37.59982332626106 40.0 37.0 41.0 33.0 41.0 42-43 37.39581110760383 40.0 37.0 41.0 33.0 41.0 44-45 37.08196682555982 40.0 36.0 41.0 31.5 41.0 46-47 36.71702334384186 39.5 36.0 41.0 31.0 41.0 48-49 36.38158364399409 39.0 36.0 41.0 30.0 41.0 50-51 36.125759004500416 39.0 35.5 40.5 30.0 41.0 52-53 35.88185701214274 39.0 35.0 40.0 29.0 41.0 54-55 35.653720137609724 39.0 35.0 40.0 28.5 41.0 56-57 35.342913538416056 38.0 35.0 40.0 28.0 41.0 58-59 35.06751793707937 38.0 34.0 40.0 27.0 41.0 60-61 34.700450774421 38.0 34.0 40.0 26.5 41.0 62-63 34.32400252257868 37.0 34.0 40.0 26.0 41.0 64-65 33.897537222686154 37.0 33.0 39.5 25.5 41.0 66-67 33.439104164918575 36.0 33.0 39.0 24.5 40.0 68-69 32.966674724534144 35.5 32.0 39.0 23.0 40.0 70-71 32.511194442335636 35.0 32.0 38.0 22.0 40.0 72-73 32.00807717971975 35.0 31.0 37.5 21.0 39.5 74-75 31.509949801483444 35.0 31.0 37.0 19.5 39.0 76-77 30.227120317946138 33.5 29.5 35.5 19.5 38.0 78-79 30.868868528899775 34.0 31.0 36.0 19.0 38.0 80-81 30.66375884201119 34.0 31.0 35.5 18.0 37.0 82-83 30.24706320697798 34.0 30.0 35.0 15.0 37.0 84-85 29.796538846246566 34.0 30.0 35.0 8.5 36.0 86-87 29.39937588153736 34.0 29.0 35.0 6.0 36.0 88-89 28.92466826225761 34.0 29.0 35.0 2.0 35.5 90-91 28.33442147841224 33.0 28.5 35.0 2.0 35.0 92-93 27.790921979864656 33.0 27.0 35.0 2.0 35.0 94-95 27.25793500164487 33.0 26.0 35.0 2.0 35.0 96-97 26.544107168305544 32.5 25.0 34.0 2.0 35.0 98-99 25.501538986093852 32.0 22.0 34.0 2.0 35.0 100-101 22.52243916310056 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18755706266382788 1101 2 0.18570105580905505 1101 3 0.17705683649069215 1101 4 0.11334011922529896 1101 5 0.06596371098582665 1101 6 0.09401784733390883 1101 7 0.08450278554330026 1101 8 0.04113572734763693 1101 9 0.1881813817764666 1101 10-11 0.1407314758565974 1101 12-13 0.13207842874535203 1101 14-15 0.20611404706881586 1101 16-17 0.1008834930932423 1101 18-19 0.093620487263955 1101 20-21 0.1541534148941892 1101 22-23 0.12532878447655804 1101 24-25 0.06889563190433279 1101 26-27 0.1179461266202324 1101 28-29 -0.009071642862366502 1101 30-31 0.25016119871473563 1101 32-33 0.20042961061907505 1101 34-35 0.1609756106491318 1101 36-37 0.27897527993633986 1101 38-39 0.06781924479307122 1101 40-41 0.32768906342498383 1101 42-43 0.29299480401201805 1101 44-45 0.19068131405968103 1101 46-47 0.3871531983755503 1101 48-49 0.3220831341292083 1101 50-51 0.40537888953701895 1101 52-53 0.4333260173168014 1101 54-55 0.29886524855168517 1101 56-57 0.35475158335587764 1101 58-59 0.38116395536320624 1101 60-61 0.38735915444506475 1101 62-63 0.46364664461122373 1101 64-65 0.514468446523459 1101 66-67 0.29995011733334564 1101 68-69 0.2768204311209814 1101 70-71 0.32535730093524506 1101 72-73 0.2698939103465392 1101 74-75 0.41233509845958594 1101 76-77 0.44579389965958427 1101 78-79 0.6173434182348423 1101 80-81 0.579954526481476 1101 82-83 0.4197117351148947 1101 84-85 0.5956673589633716 1101 86-87 0.7015928419907382 1101 88-89 0.8249348510748398 1101 90-91 1.0090135816964612 1101 92-93 0.9922863476690651 1101 94-95 1.180697834682615 1101 96-97 1.4129039319019938 1101 98-99 1.4132565359038658 1101 100-101 1.9508159052286942 1102 1 0.24903450440899633 1102 2 0.22685127891267598 1102 3 0.17408212156753677 1102 4 0.06128260807011543 1102 5 0.07984571396053752 1102 6 0.06922855630762825 1102 7 0.12862772357006946 1102 8 0.07931123552810249 1102 9 0.0766295721582182 1102 10-11 0.08892185761167326 1102 12-13 0.09464659929567887 1102 14-15 0.2361090892106148 1102 16-17 0.29473574891872545 1102 18-19 0.2153359062029736 1102 20-21 0.20224463948516558 1102 22-23 0.21754494709431782 1102 24-25 0.056748879301459 1102 26-27 0.12711816429995082 1102 28-29 0.12627788614111068 1102 30-31 0.2655305591510242 1102 32-33 0.27752430571079145 1102 34-35 0.24154080648452947 1102 36-37 0.3637546552462112 1102 38-39 0.3804122046344176 1102 40-41 0.34628103169468716 1102 42-43 0.5522907881468768 1102 44-45 0.509719489567857 1102 46-47 0.5120912251479908 1102 48-49 0.5013097082491882 1102 50-51 0.4242187507169888 1102 52-53 0.48637510011303675 1102 54-55 0.5090784364545087 1102 56-57 0.5639731996176494 1102 58-59 0.662274515601176 1102 60-61 0.5442753666414006 1102 62-63 0.5219014785229703 1102 64-65 0.616352432641456 1102 66-67 0.6546848719193576 1102 68-69 0.5086003022166636 1102 70-71 0.5380494179407052 1102 72-73 0.6285949514967584 1102 74-75 0.6780762982612742 1102 76-77 0.6659228039729221 1102 78-79 0.6406453517995452 1102 80-81 0.7792604263327441 1102 82-83 0.6398406394282325 1102 84-85 0.7528314640699669 1102 86-87 0.8148799019808237 1102 88-89 0.8683161103708237 1102 90-91 1.059335842479804 1102 92-93 1.1144975524286096 1102 94-95 1.2627503879795903 1102 96-97 1.3343218793486962 1102 98-99 1.6797414144363394 1102 100-101 2.2921644426605248 1103 1 0.15147366710046128 1103 2 0.15715182965158192 1103 3 0.07709818073837482 1103 4 0.10360018366512946 1103 5 0.041490973412336984 1103 6 0.0707378555583773 1103 7 0.10725987243303337 1103 8 0.0696736128050901 1103 9 0.04023775886164316 1103 10-11 0.08910503457072849 1103 12-13 0.05201333312722767 1103 14-15 0.08291817370302113 1103 16-17 0.13266506529393496 1103 18-19 0.11516596827851089 1103 20-21 0.1984362598419125 1103 22-23 0.10945821440669334 1103 24-25 0.03976396218595113 1103 26-27 0.006204976619009983 1103 28-29 -0.04510967562564616 1103 30-31 -0.05376401479243498 1103 32-33 -0.06515935261166561 1103 34-35 -0.007439948100994798 1103 36-37 0.08603028011261671 1103 38-39 0.03976913270503957 1103 40-41 0.19964343830957176 1103 42-43 0.11473095670913125 1103 44-45 0.10486796275215937 1103 46-47 0.1329648436473363 1103 48-49 0.1857546068694873 1103 50-51 -0.05041407562952571 1103 52-53 -0.02805791296056981 1103 54-55 0.07140729358066977 1103 56-57 0.11908098927330002 1103 58-59 0.05675780742179626 1103 60-61 0.10216791449483509 1103 62-63 -0.056918482193829334 1103 64-65 0.02260465917834864 1103 66-67 0.06396494209364079 1103 68-69 0.1584805961971938 1103 70-71 0.20999575572049167 1103 72-73 0.15090728571606604 1103 74-75 0.28732557596962494 1103 76-77 0.37274302700504336 1103 78-79 0.3251987266809273 1103 80-81 0.3583745056392438 1103 82-83 0.45676660955732373 1103 84-85 0.46604044580577053 1103 86-87 0.3791644135183816 1103 88-89 0.5611501685638984 1103 90-91 0.7565030342291017 1103 92-93 0.8117690293107138 1103 94-95 0.7587654878751344 1103 96-97 0.8550065765778925 1103 98-99 1.1442472422001977 1103 100-101 1.8417475856467824 1104 1 0.13799468607221144 1104 2 0.16796721733800268 1104 3 0.19859288123396368 1104 4 0.1781482896135671 1104 5 0.1000405036009937 1104 6 0.09792083131632978 1104 7 0.07863843436690843 1104 8 0.0681183262275411 1104 9 0.13094926437600662 1104 10-11 0.09936906249884458 1104 12-13 0.12402228897730794 1104 14-15 0.25415906077509476 1104 16-17 0.21288994185385235 1104 18-19 0.05811229666949913 1104 20-21 0.13896915120702857 1104 22-23 0.024543351362829924 1104 24-25 0.010167041470516835 1104 26-27 0.16919095923048388 1104 28-29 0.017241214702266916 1104 30-31 0.07498902835280319 1104 32-33 0.11621400810256688 1104 34-35 0.16094687127699814 1104 36-37 0.2481843977363667 1104 38-39 0.25554330428129646 1104 40-41 0.1770689260128009 1104 42-43 0.16171056681066176 1104 44-45 0.2846829892991991 1104 46-47 0.3504921411616664 1104 48-49 0.4251437886373921 1104 50-51 0.37602244384025596 1104 52-53 0.16406810588674148 1104 54-55 0.22168965276044617 1104 56-57 0.3299745730304622 1104 58-59 0.20151933099312913 1104 60-61 0.30371466782141 1104 62-63 0.2558638960333752 1104 64-65 0.14507395344293172 1104 66-67 0.33473007502696106 1104 68-69 0.23260838874166723 1104 70-71 0.22589084041555196 1104 72-73 0.426631735867403 1104 74-75 0.2862493958794232 1104 76-77 0.33356943680731277 1104 78-79 0.2401578965987774 1104 80-81 0.19144176746630492 1104 82-83 0.01726227969434646 1104 84-85 0.10348965899431661 1104 86-87 0.14789769304482903 1104 88-89 0.27234813033595273 1104 90-91 0.4850840663359648 1104 92-93 0.6028413948345523 1104 94-95 0.6477261785402142 1104 96-97 0.9801525434231628 1104 98-99 1.2695994292775055 1104 100-101 1.603322965998668 1105 1 0.04233268013959446 1105 2 -0.01889374337957861 1105 3 0.009287259739707565 1105 4 -0.07625308553476629 1105 5 -0.09361440812639898 1105 6 -0.057921497654660925 1105 7 -0.015266804656228317 1105 8 0.05756493665847984 1105 9 0.003738452879701981 1105 10-11 0.07030221637233325 1105 12-13 0.0892953711201443 1105 14-15 0.08790068123138184 1105 16-17 0.07630291315573601 1105 18-19 0.15776552569447233 1105 20-21 0.07140955460802445 1105 22-23 0.015559036515249147 1105 24-25 -0.021469415945333026 1105 26-27 -0.08324890600412971 1105 28-29 -0.05056428663702661 1105 30-31 -0.08050828800857346 1105 32-33 0.026204214539994553 1105 34-35 0.03418297765917799 1105 36-37 0.05229822685414831 1105 38-39 -0.0329351927745094 1105 40-41 -0.09788694167925627 1105 42-43 -0.08866575003185773 1105 44-45 -0.02380877009459681 1105 46-47 0.18368613735614048 1105 48-49 0.24777720194970954 1105 50-51 0.2519303331996383 1105 52-53 0.2667194811682805 1105 54-55 0.2746264427204963 1105 56-57 0.3157708434841169 1105 58-59 0.17675808238316648 1105 60-61 0.3067680960212158 1105 62-63 0.4912377591568813 1105 64-65 0.3448624966798022 1105 66-67 0.477778430608943 1105 68-69 0.3333201004677875 1105 70-71 0.38715466289949774 1105 72-73 0.3182150533488013 1105 74-75 0.40395264560514477 1105 76-77 0.4234907792607032 1105 78-79 0.5247249630538029 1105 80-81 0.49642625388773 1105 82-83 0.3902155823042399 1105 84-85 0.5193352222920069 1105 86-87 0.6016661365663971 1105 88-89 0.935776474014336 1105 90-91 0.9139108955821627 1105 92-93 0.8449178272496489 1105 94-95 1.0246732352229877 1105 96-97 1.4333214109772214 1105 98-99 1.375370171462233 1105 100-101 2.000751649627702 1106 1 -0.14601991807292336 1106 2 -0.006591981765289745 1106 3 0.005357546008731617 1106 4 0.10012416924040934 1106 5 -0.022197947904416537 1106 6 -0.038945796201183214 1106 7 -0.03155667979633847 1106 8 -0.03300637414607621 1106 9 -0.07055821751382751 1106 10-11 -0.268398678780855 1106 12-13 -0.39215380709317316 1106 14-15 -0.18891744967612567 1106 16-17 -0.2173659566220394 1106 18-19 -0.13072621637705595 1106 20-21 -0.03433850867703825 1106 22-23 -0.0662018047577746 1106 24-25 0.03247266894991441 1106 26-27 0.01306744935213544 1106 28-29 0.010003776913244167 1106 30-31 -0.06456077108603608 1106 32-33 -0.014434284241083617 1106 34-35 -0.31909106436554424 1106 36-37 0.003503646580810482 1106 38-39 0.13112605309341774 1106 40-41 -0.02817521942981216 1106 42-43 0.030593119895428345 1106 44-45 -0.05971251256222132 1106 46-47 0.13413109018913616 1106 48-49 0.1953190925175221 1106 50-51 0.16563292815361308 1106 52-53 0.17772496482437816 1106 54-55 0.13515376747667318 1106 56-57 0.16971112278184108 1106 58-59 0.18394309095834416 1106 60-61 0.3210809829374952 1106 62-63 0.2835594839087747 1106 64-65 0.17084154667031015 1106 66-67 0.3330202918075855 1106 68-69 0.4755876431877937 1106 70-71 0.545325993227074 1106 72-73 0.5152163226274382 1106 74-75 0.535084588797659 1106 76-77 0.2709163780631414 1106 78-79 0.3909598097881961 1106 80-81 0.3821568987991988 1106 82-83 0.3188387605903493 1106 84-85 0.40635842111678855 1106 86-87 0.7041347833528704 1106 88-89 0.6798900145004829 1106 90-91 0.8049227721819854 1106 92-93 0.6972246050395547 1106 94-95 0.4915866480759661 1106 96-97 0.6440644058936158 1106 98-99 0.8378777853002219 1106 100-101 1.2123524444291576 1107 1 0.04466637039026011 1107 2 0.06891041632969319 1107 3 0.05865416826154757 1107 4 0.014471306894591862 1107 5 -0.037810507993363274 1107 6 0.019502108308607546 1107 7 -0.034408056562490685 1107 8 -0.02062242247772872 1107 9 0.012920962432858119 1107 10-11 -0.03600299037764643 1107 12-13 -0.060681042384807427 1107 14-15 0.004042579582325345 1107 16-17 -0.018278931982081303 1107 18-19 0.0071950819438910685 1107 20-21 -0.004168314520619276 1107 22-23 -0.11492794348989577 1107 24-25 -0.2276128867292826 1107 26-27 -0.1247224504428317 1107 28-29 0.005879818361570699 1107 30-31 0.03003624803633187 1107 32-33 0.13711939550706376 1107 34-35 0.06147342600225869 1107 36-37 -0.06850705851747563 1107 38-39 0.05181769813108872 1107 40-41 0.28206029445774305 1107 42-43 0.24351321780314805 1107 44-45 0.2730303899765829 1107 46-47 0.3259713567355007 1107 48-49 0.30672721605169073 1107 50-51 0.4536198251041057 1107 52-53 0.4349510930618621 1107 54-55 0.3140308650011221 1107 56-57 0.2998193842413315 1107 58-59 0.4940663266743641 1107 60-61 0.5481190905226967 1107 62-63 0.38084672252418983 1107 64-65 0.46823608928986715 1107 66-67 0.5051599802291875 1107 68-69 0.5394049170047879 1107 70-71 0.6775705589492063 1107 72-73 0.6858636170685415 1107 74-75 0.6593132693868284 1107 76-77 0.6006652918688715 1107 78-79 0.6212948127741349 1107 80-81 0.7002584977496227 1107 82-83 0.6815724422304079 1107 84-85 0.783648730489876 1107 86-87 0.9256859242629858 1107 88-89 0.9799892965814898 1107 90-91 1.1089050675963499 1107 92-93 1.1469235529025177 1107 94-95 1.169727683835628 1107 96-97 1.3906023997630257 1107 98-99 1.7500485090760804 1107 100-101 2.2946268982842675 1108 1 -0.07777130273993293 1108 2 0.0021877883882268634 1108 3 -0.06131561265006269 1108 4 0.004711660569853393 1108 5 0.015408664339382483 1108 6 -0.07227997848733025 1108 7 -0.09166763293563918 1108 8 -0.007191716526250502 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0.6180322177061903 2316 78-79 0.5576829540553696 2316 80-81 0.622754012103691 2316 82-83 0.5618717344951634 2316 84-85 0.93629816895346 2316 86-87 1.1217190349753707 2316 88-89 0.9810384393247134 2316 90-91 1.1483820745075697 2316 92-93 1.1342158841093202 2316 94-95 1.2020019063236695 2316 96-97 1.4022821080299046 2316 98-99 1.578288142249928 2316 100-101 1.9860476850997912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 8.0 5 30.0 6 63.0 7 137.0 8 304.0 9 605.0 10 1094.0 11 1837.0 12 2837.0 13 4141.0 14 5574.0 15 7711.0 16 10993.0 17 15231.0 18 13818.0 19 8777.0 20 7723.0 21 7946.0 22 8315.0 23 9194.0 24 10586.0 25 12534.0 26 15264.0 27 18958.0 28 23872.0 29 29449.0 30 36705.0 31 46566.0 32 59724.0 33 78849.0 34 109077.0 35 155922.0 36 231758.0 37 326861.0 38 222942.0 39 16223.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.907339536947525 19.866644725051348 15.234720874004715 21.99129486399641 2 15.676015124817033 21.211503704621222 39.330850711023565 23.781630459538185 3 19.433645126188868 25.00635972185041 30.092738729307595 25.46725642265313 4 13.030104459263816 17.450077848322966 37.29274299132015 32.22707470109307 5 13.660392134925742 37.760267869629324 33.92029706464859 14.659042930796351 6 31.51911446034264 37.07344912688677 17.08267904781312 14.324757364957467 7 28.514129237539276 33.17550568112954 20.368291159387283 17.942073921943905 8 26.624392829696635 34.980467995615456 19.3926232554051 19.002515919282807 9 26.278503873769843 17.11830680896461 20.439879651420647 36.16330966584489 10-11 24.925214998554914 27.227900466024437 27.034012192019645 20.812872343401008 12-13 26.110264033908543 24.35769663521844 27.857670162899662 21.674369167973357 14-15 22.644039375668438 25.753943360516985 26.584702837834417 25.017314425980164 16-17 21.786842101758214 29.391837446841386 26.968417155133263 21.852903296267144 18-19 21.604940738047386 27.420423564134634 29.965843965056827 21.00879173276116 20-21 22.453281709551632 26.332257504910483 30.185028503959188 21.029432281578693 22-23 22.062070008064584 26.293413743892458 30.161684626075587 21.48283162196737 24-25 22.27832025982311 26.526184424065203 30.106510428285933 21.088984887825752 26-27 21.927248365412407 26.833908052866207 30.072182908653556 21.16666067306783 28-29 21.47109113945547 27.048111230060485 30.27509494213566 21.20570268834838 30-31 21.668883698241746 26.552435432770665 30.267225835881952 21.511455033105637 32-33 21.60388776881161 26.80729139786199 30.140830063281125 21.447990770045266 34-35 22.261823422156336 26.60328254879005 29.779314322093608 21.355579706959997 36-37 22.120085573249206 26.454886748632745 29.905952668337065 21.519075009780988 38-39 21.871238478819606 26.88160550173094 30.073223446553143 21.173932572896305 40-41 21.94350435990565 26.73423954375156 29.977031747997852 21.345224348344942 42-43 21.823259060252504 26.826485766860625 29.5848703756366 21.765384797250274 44-45 22.00080845050538 26.990359860365814 29.30989359619296 21.698938092935848 46-47 22.15387463556463 27.29689179416078 28.80584401833198 21.743389551942613 48-49 22.43633285785256 27.41935806099739 28.563208975798425 21.581100105351624 50-51 22.089578180626756 27.486051897994056 28.55068931393585 21.873680607443337 52-53 22.514847120277988 27.660960897366742 28.093888140816897 21.730303841538372 54-55 22.080288325148935 27.493277341143475 28.322205484943773 22.104228848763814 56-57 22.293688625354445 27.862008020574862 28.183956452886783 21.660346901183907 58-59 22.546978699260407 27.426716692038962 28.209228855423213 21.81707575327742 60-61 22.3411368119347 27.43564028081335 28.74201372101665 21.481209186235297 62-63 22.126304741888525 27.45984718021254 28.76998317836922 21.643864899529714 64-65 22.023816658875177 27.5050644695992 28.478434882441704 21.99268398908392 66-67 22.02145257378942 27.85694688067297 28.098882686772708 22.022717858764896 68-69 22.065071608470216 27.630427573090188 28.392595285947746 21.91190553249185 70-71 22.119478862415576 27.698020428358898 28.250683586856486 21.93181712236904 72-73 22.283825486103805 27.438077454293126 28.38797603144308 21.890121028159992 74-75 22.06473863873983 27.955239545083437 27.90945620715512 22.070565609021614 76-77 22.207782434914407 27.665722364511286 28.09185702546153 22.03463817511278 78-79 22.10442863060205 27.747533027267558 28.041878268930326 22.106160073200066 80-81 22.02335050125263 28.13148042337767 27.88851241111373 21.956656664255966 82-83 22.182180695648178 27.804816814733 27.97096898681471 22.04203350280411 84-85 21.981170555476936 27.79092823667496 27.7852677493736 22.442633458474496 86-87 22.086881125810613 27.713836490552318 28.031289831503997 22.16799255213307 88-89 22.045492988323417 27.60865135272283 27.876725282658004 22.46913037629575 90-91 22.010099146660735 27.671188994034683 28.000490172246263 22.31822168705832 92-93 22.114035432376077 27.46928152976017 28.055248295009193 22.361434742854556 94-95 21.96524728329997 27.37886894178224 27.925105784483346 22.730777990434444 96-97 21.980930157601232 27.377303984049416 27.972354189225367 22.669411669123985 98-99 21.835677276091783 27.567623722518732 27.671810055229745 22.92488894615974 100-101 21.781375709300157 27.42337560273863 28.03210831979114 22.763140368170074 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 555.0 1 392.0 2 300.5 3 418.5 4 375.0 5 340.0 6 620.0 7 1321.5 8 2212.5 9 2566.0 10 2367.0 11 2121.0 12 2124.0 13 2343.0 14 2480.5 15 2573.0 16 2656.0 17 2725.0 18 2836.0 19 2995.5 20 3277.5 21 3656.5 22 4117.5 23 4895.0 24 5969.5 25 7386.5 26 9279.0 27 11394.0 28 13061.0 29 14846.0 30 17365.0 31 20012.5 32 23091.0 33 26437.0 34 29966.0 35 33010.0 36 36559.0 37 39940.0 38 42138.5 39 42986.0 40 43566.5 41 46672.0 42 50152.0 43 52922.0 44 54993.5 45 56479.5 46 56013.0 47 57592.0 48 64831.0 49 64918.5 50 60141.0 51 57348.5 52 52790.5 53 50252.5 54 47718.0 55 43456.0 56 38449.5 57 33110.0 58 28980.5 59 25417.0 60 21722.0 61 17408.0 62 13589.5 63 10469.0 64 7831.5 65 5884.5 66 4276.0 67 3354.5 68 2343.5 69 1383.0 70 990.5 71 765.5 72 629.5 73 527.0 74 411.0 75 311.0 76 202.5 77 123.0 78 76.5 79 44.0 80 27.5 81 14.5 82 6.5 83 5.5 84 2.0 85 1.5 86 1.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045616853063121736 2 0.0 3 0.0 4 0.0 5 7.991273529305998E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004628279252389724 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.9967275734897494E-4 22-23 7.325334068530498E-4 24-25 6.659394607754998E-5 26-27 0.0023973820587917995 28-29 0.004361903468079524 30-31 3.662667034265249E-4 32-33 3.662667034265249E-4 34-35 0.0 36-37 3.329697303877499E-5 38-39 9.989091911632498E-5 40-41 0.0 42-43 0.007125552230297848 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.663757843101999E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.6648486519387496E-4 84-85 3.329697303877499E-5 86-87 0.0 88-89 0.0 90-91 0.008324243259693748 92-93 0.0011320970833183497 94-95 0.0 96-97 0.0 98-99 9.989091911632498E-5 100-101 0.010521843480252898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1501638.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.31887202253846 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0658342121271 43.73832764135902 2 12.029842515935227 13.309546371806258 3 3.6931803016258957 6.129077053854088 4 1.6132519385089676 3.5697311010595865 5 0.893562070351667 2.471542290698919 6 0.5281404438690718 1.75296801745919 7 0.35912806009079984 1.390659143710582 8 0.2577576976083214 1.140709206945512 9 0.19377509675565024 0.9647477800722741 >10 1.1912633121984435 12.382814553858942 >50 0.11111815416475383 4.297336378785907 >100 0.0585582188941027 5.734919780751397 >500 0.0033806152725771926 1.154798010860519 >1k 9.658900778791978E-4 0.9877563784046395 >5k 2.4147251946979946E-4 0.9750662903732051 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8144 0.5423410968555671 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6455 0.4298639219305851 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4644 0.30926228558414215 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2882 0.19192375259549907 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1702 0.11334289622399008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 6.659394607754998E-5 0.0 26-27 0.0 0.0 0.0 1.3318789215509996E-4 0.0 28-29 0.0 0.0 0.0 4.994545955816249E-4 0.0 30-31 0.0 0.0 0.0 0.0014650668137060996 0.0 32-33 0.0 0.0 0.0 0.0025971638970244495 0.0 34-35 0.0 0.0 0.0 0.003296400330838724 0.0 36-37 0.0 0.0 0.0 0.005127733847971348 0.0 38-39 0.0 0.0 0.0 0.008124461421461098 0.0 40-41 0.0 0.0 0.0 0.012719443700812048 0.0 42-43 0.0 0.0 0.0 0.01688156533065892 0.0 44-45 0.0 0.0 0.0 0.01914575949729562 0.0 46-47 0.0 0.0 0.0 0.02284172350459964 0.0 48-49 0.0 0.0 0.0 0.02870199075942404 0.0 50-51 0.0 0.0 0.0 0.03659337336961371 0.0 52-53 0.0 0.0 0.0 0.05444055091839711 0.0 54-55 0.0 0.0 0.0 0.06779263710694589 0.0 56-57 0.0 0.0 0.0 0.09832596138350255 0.0 58-59 0.0 0.0 0.0 0.15336585781659762 0.0 60-61 0.0 0.0 0.0 0.20590848127178457 0.0 62-63 0.0 0.0 0.0 0.2389723754992881 0.0 64-65 0.0 0.0 0.0 0.2971088904249892 0.0 66-67 0.0 0.0 0.0 0.3924714212080408 0.0 68-69 0.0 0.0 0.0 0.5259922830935285 0.0 70-71 0.0 0.0 0.0 0.6592134722216674 0.0 72-73 0.0 0.0 0.0 0.8061197172687424 0.0 74-75 0.0 0.0 0.0 0.9800298074502642 0.0 76-77 0.0 0.0 0.0 1.1232067915169968 0.0 78-79 0.0 0.0 0.0 1.3116676589164633 0.0 80-81 0.0 0.0 0.0 1.5537366529083574 0.0 82-83 0.0 0.0 0.0 1.7751282266431723 0.0 84-85 0.0 0.0 0.0 2.036376277105401 0.0 86-87 0.0 0.0 0.0 2.357492285091347 0.0 88-89 0.0 0.0 0.0 2.760418955833563 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10060 0.0 27.776262 1 GGTATCA 7025 0.0 25.908775 1 CGATCGT 215 0.0 24.302233 24-25 ATTCGCG 200 0.0 23.749117 80-81 CGCGATC 225 0.0 23.222132 22-23 CCCGCGA 225 0.0 23.221745 20-21 GTACATG 6300 0.0 23.006668 1 GTCTAGC 290 3.6379788E-12 22.94164 1 ATCAACG 12055 0.0 22.931541 3 TCAACGC 12110 0.0 22.827393 4 TATCAAC 12200 0.0 22.658993 2 TACATGG 6290 0.0 22.654165 2 CAACGCA 12275 0.0 22.481853 5 GAGTACT 6905 0.0 22.05028 12-13 AACGCAG 12550 0.0 22.02707 6 AGTACTT 7015 0.0 21.975403 12-13 CGTTAGG 130 2.9329045E-4 21.933216 1 TAACGCG 65 2.9437782E-4 21.92226 76-77 ACATGGG 6095 0.0 21.898285 3 TCGCGGA 220 0.0 21.590107 82-83 >>END_MODULE