FastQCFastQC Report
Thu 26 May 2016
SRR937154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479696
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69750.4713806079086515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52250.3531130718742228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38020.25694466971594165No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22020.14881435105589255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21390.14455671975865314No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15040.10164249954044614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88450.030.0589561
GGTATCA59700.026.6888831
GTACATG64400.026.2920251
GCGTTCG559.469661E-525.9138834-35
ATCAACG104650.025.2574313
TCAACGC104800.025.2247054
TATCAAC105600.025.1652682
CAACGCA105350.025.090465
AACGCAG107150.024.6472326
TACATGG65450.024.405252
ACATGGG65850.023.1019083
GAGTACT54550.022.85627212-13
ACGCAGA118650.022.0581677
CGCAGAG118800.021.9510948
GTACTTT57650.021.6261314-15
CGAACCG550.002879299721.58977776-77
AGTACTT56850.021.51382412-13
GCAGAGT123500.020.61579
GTACCTG12750.020.1438961
AGAGTAC93250.020.04386510-11
Produced by FastQC (version 0.11.5)