##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937154_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1479696 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.633017863128643 33.0 31.0 34.0 30.0 34.0 2 31.743945377969528 33.0 31.0 34.0 30.0 34.0 3 31.782510056119634 33.0 31.0 34.0 30.0 34.0 4 35.396739600566605 37.0 35.0 37.0 33.0 37.0 5 35.39633276024264 37.0 35.0 37.0 33.0 37.0 6 35.43321060542166 37.0 35.0 37.0 33.0 37.0 7 35.369429261145534 37.0 35.0 37.0 33.0 37.0 8 35.36488778776181 37.0 35.0 37.0 33.0 37.0 9 36.96682832149306 39.0 37.0 39.0 33.0 39.0 10-11 36.84030909051589 39.0 37.0 39.0 33.0 39.0 12-13 36.70458323871931 39.0 37.0 39.0 32.5 39.0 14-15 37.864714779251955 40.0 38.0 41.0 33.0 41.0 16-17 37.828170786431805 40.0 38.0 41.0 33.0 41.0 18-19 37.79983557433419 40.0 38.0 41.0 33.0 41.0 20-21 37.740385187227645 40.0 38.0 41.0 32.0 41.0 22-23 37.58532225538219 40.0 37.0 41.0 32.0 41.0 24-25 37.41734214325105 40.0 37.0 41.0 32.0 41.0 26-27 37.260324417988556 40.0 37.0 41.0 31.5 41.0 28-29 37.318182248245584 40.0 37.0 41.0 32.0 41.0 30-31 37.15844470756155 39.5 37.0 41.0 31.0 41.0 32-33 36.96751663855278 39.0 36.0 41.0 31.0 41.0 34-35 36.83504652306961 39.0 36.0 41.0 30.5 41.0 36-37 36.840178658319005 39.0 36.0 41.0 30.5 41.0 38-39 36.75646315189066 39.5 36.0 41.0 30.0 41.0 40-41 36.58456669478055 39.0 36.0 41.0 30.0 41.0 42-43 36.345221923962754 39.0 35.0 41.0 30.0 41.0 44-45 36.121254636087414 39.0 35.0 41.0 29.5 41.0 46-47 35.77739076134557 39.0 35.0 41.0 27.5 41.0 48-49 35.46146438187303 39.0 35.0 40.5 27.0 41.0 50-51 34.583663468712494 38.0 34.0 39.5 25.0 40.5 52-53 34.685751330002915 38.0 34.5 40.0 26.0 40.5 54-55 35.00742956661368 39.0 35.0 40.0 26.0 41.0 56-57 34.79918442707151 38.0 34.5 40.0 25.0 41.0 58-59 34.54840757831338 38.0 34.0 40.0 24.0 41.0 60-61 34.26450365480477 38.0 34.0 40.0 23.0 41.0 62-63 33.935712132762404 37.5 34.0 40.0 22.5 41.0 64-65 33.566380864718155 37.0 33.0 40.0 22.0 41.0 66-67 33.18620378780506 36.5 33.0 39.0 20.5 41.0 68-69 32.70469981671911 36.0 32.0 39.0 19.5 40.5 70-71 32.26036057406387 35.5 32.0 39.0 18.0 40.0 72-73 31.859572168877932 35.0 31.0 38.0 15.5 40.0 74-75 31.39887821552535 35.0 31.0 37.0 12.0 39.0 76-77 30.966490076340005 35.0 31.0 37.0 7.5 39.0 78-79 30.490730528432866 34.5 30.5 36.0 7.0 39.0 80-81 29.992472102377786 34.0 30.0 36.0 2.0 37.5 82-83 29.511903458548243 34.0 29.0 35.0 2.0 37.0 84-85 29.0765052416172 34.0 29.0 35.0 2.0 36.5 86-87 28.64449589645441 34.0 29.0 35.0 2.0 36.0 88-89 28.24054366572593 34.0 28.5 35.0 2.0 36.0 90-91 27.81461969215298 34.0 27.0 35.0 2.0 35.0 92-93 27.352712313880687 33.0 26.0 35.0 2.0 35.0 94-95 26.81127407251219 33.0 25.5 35.0 2.0 35.0 96-97 26.172682429363867 33.0 24.5 35.0 2.0 35.0 98-99 25.39344601864167 32.0 21.5 34.5 2.0 35.0 100-101 23.474468066413642 30.5 8.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.023174372580054836 1101 2 -0.05763117289966857 1101 3 0.054565506254263596 1101 4 0.048936022098224896 1101 5 0.13648542537930552 1101 6 0.07913086978423678 1101 7 -0.021238611998029455 1101 8 0.046303574428890215 1101 9 -0.02173838752183599 1101 10-11 0.04623588821905855 1101 12-13 -0.07994203711334791 1101 14-15 -0.03272234191523182 1101 16-17 0.18610518595148307 1101 18-19 0.09864983830382101 1101 20-21 0.08639239885569339 1101 22-23 0.13028467951445322 1101 24-25 0.1407818769232705 1101 26-27 0.2251146935560442 1101 28-29 0.06938579066385842 1101 30-31 0.0991366160778071 1101 32-33 0.12165160020147425 1101 34-35 0.10056800547894085 1101 36-37 0.16374288892262712 1101 38-39 0.16984208235011522 1101 40-41 -0.0018153731723131727 1101 42-43 0.05973092064410679 1101 44-45 0.11939278666471864 1101 46-47 0.3230599105081282 1101 48-49 0.33067417854739034 1101 50-51 0.1774532269924336 1101 52-53 0.2604829429235238 1101 54-55 0.3549482900026959 1101 56-57 0.32310284917768683 1101 58-59 0.44818941362301956 1101 60-61 0.2641400417179298 1101 62-63 0.37523781644562604 1101 64-65 0.41865852660986747 1101 66-67 0.3967261291938655 1101 68-69 0.5267345088480653 1101 70-71 0.5146675603897819 1101 72-73 0.5173224035076629 1101 74-75 0.4913139637236412 1101 76-77 0.438020449626606 1101 78-79 0.316881853677458 1101 80-81 0.2834277593068961 1101 82-83 0.27880438254103623 1101 84-85 0.42336096381475485 1101 86-87 0.3219149450608363 1101 88-89 0.306903787385604 1101 90-91 0.2924721199974556 1101 92-93 0.49396938730377116 1101 94-95 0.5382317395181175 1101 96-97 0.3921297293578192 1101 98-99 0.4872605846014899 1101 100-101 0.7933366687595829 1102 1 0.007731228738126816 1102 2 -0.026804990679934804 1102 3 0.1453517812414198 1102 4 0.04142633358664227 1102 5 0.032011412256281346 1102 6 -0.10937850305717944 1102 7 0.0045692299012998205 1102 8 0.08005512220282895 1102 9 0.042703755727188764 1102 10-11 0.12760400972528885 1102 12-13 0.10581052173700556 1102 14-15 0.19449972564284934 1102 16-17 0.21549920406000211 1102 18-19 0.25619080262377025 1102 20-21 0.26339868080855666 1102 22-23 0.2363639511666875 1102 24-25 0.24800687986448366 1102 26-27 0.2385957900381257 1102 28-29 0.3160862220021059 1102 30-31 0.2147679971872165 1102 32-33 0.19594090550578613 1102 34-35 0.18876729935622194 1102 36-37 0.29625167468741864 1102 38-39 0.31980195379897225 1102 40-41 0.1980762972121184 1102 42-43 0.3186366051533227 1102 44-45 0.21653801008582008 1102 46-47 0.32429824614589364 1102 48-49 0.21833042654532875 1102 50-51 0.18192793901619808 1102 52-53 0.2895504579003827 1102 54-55 0.26392893161688136 1102 56-57 0.31483496984057524 1102 58-59 0.4094333870601332 1102 60-61 0.41026527774253196 1102 62-63 0.24209361341961966 1102 64-65 0.2074403542586154 1102 66-67 0.2660757205116582 1102 68-69 0.24917225135206422 1102 70-71 0.31260126033538427 1102 72-73 0.0706484139142951 1102 74-75 0.2551927929717017 1102 76-77 0.37855948847500187 1102 78-79 0.2870654665516774 1102 80-81 0.46383931865929284 1102 82-83 0.4965299032512114 1102 84-85 0.6037896462354233 1102 86-87 0.4840108785068473 1102 88-89 0.44706343961259165 1102 90-91 0.3810985587137594 1102 92-93 0.5804778073105048 1102 94-95 0.3460539531464448 1102 96-97 0.46590114920962833 1102 98-99 0.5433992045753762 1102 100-101 0.8402719307483473 1103 1 0.041301322015257824 1103 2 -0.06902395614297063 1103 3 0.024522468474039272 1103 4 -0.16139398762332746 1103 5 0.045697055994232016 1103 6 0.07358421269304216 1103 7 -0.021560700279906087 1103 8 0.17261495910690883 1103 9 0.14760606504354 1103 10-11 0.2456225539055623 1103 12-13 0.19807410641315215 1103 14-15 0.20469406710437 1103 16-17 0.1020394375309408 1103 18-19 0.09443406318788305 1103 20-21 -0.2239457867717718 1103 22-23 -0.27383232500326926 1103 24-25 -0.22023137464157116 1103 26-27 0.07750507397835094 1103 28-29 -0.1900000079524986 1103 30-31 -0.19463746691305772 1103 32-33 -0.2644122029660636 1103 34-35 -0.1473639851208901 1103 36-37 -0.04045083245286918 1103 38-39 -0.12934435610745965 1103 40-41 -0.020380216588399946 1103 42-43 -0.07318127106886863 1103 44-45 -0.16176931351109403 1103 46-47 -0.0429647943935052 1103 48-49 -0.020085143988090692 1103 50-51 0.04599290652519272 1103 52-53 0.06690046358358614 1103 54-55 0.11669991776194166 1103 56-57 0.04345305597863103 1103 58-59 0.2237305195803998 1103 60-61 0.1604803931358063 1103 62-63 -0.013028768980333894 1103 64-65 0.04810526517738367 1103 66-67 0.05782564010034008 1103 68-69 0.14282930977299912 1103 70-71 0.07511495444347815 1103 72-73 0.1628098218016163 1103 74-75 0.19963062716235314 1103 76-77 0.3708104975463691 1103 78-79 0.2703608312321215 1103 80-81 0.2787111805017659 1103 82-83 0.32637507820931333 1103 84-85 0.03759698313345439 1103 86-87 0.09024970858866155 1103 88-89 0.10772658510991917 1103 90-91 0.17126431485827354 1103 92-93 0.09252003715870316 1103 94-95 0.05923427522703406 1103 96-97 0.21806221852297725 1103 98-99 0.2798876845958844 1103 100-101 0.3895483497965202 1104 1 0.02155630786854701 1104 2 -0.004476708425997344 1104 3 0.002383727186341389 1104 4 -0.02600391792488921 1104 5 0.07562080176747088 1104 6 -0.03173403081442672 1104 7 0.11321979566010043 1104 8 0.039168624600435464 1104 9 0.037329284053036815 1104 10-11 0.09326721265927063 1104 12-13 0.08114161383155505 1104 14-15 0.1445935449411877 1104 16-17 0.16750032704857887 1104 18-19 0.08673028728099297 1104 20-21 -0.026407947193185066 1104 22-23 0.05168078083253391 1104 24-25 -0.017575415770551217 1104 26-27 -0.0021506893690101947 1104 28-29 -0.024865901725647177 1104 30-31 0.05790793483055978 1104 32-33 -0.06477392173403729 1104 34-35 0.11954737365352486 1104 36-37 0.1209575417104034 1104 38-39 0.14016559834703202 1104 40-41 0.17105325936218918 1104 42-43 0.17436965134273663 1104 44-45 0.21366917704626331 1104 46-47 0.1519442583518753 1104 48-49 0.16091656971926938 1104 50-51 0.21132109873645533 1104 52-53 0.2609181584746736 1104 54-55 0.1942192976069137 1104 56-57 0.2651941366493915 1104 58-59 0.11125409743332426 1104 60-61 0.217914748942853 1104 62-63 0.11744733112777794 1104 64-65 0.14732777110059914 1104 66-67 0.2622219032981832 1104 68-69 0.3497048417123665 1104 70-71 0.32475050409497896 1104 72-73 0.30951222904289466 1104 74-75 0.22061635393529144 1104 76-77 0.299917356976362 1104 78-79 0.2403170396833474 1104 80-81 0.2771872461057008 1104 82-83 0.30811374358782473 1104 84-85 0.4885004174490071 1104 86-87 0.4935747609199943 1104 88-89 0.3040769059987447 1104 90-91 0.4136000749852471 1104 92-93 0.5990542653003068 1104 94-95 0.5802875726760242 1104 96-97 0.6361694344961109 1104 98-99 0.8027538889066044 1104 100-101 0.8769651084743195 1105 1 -0.013400608574173134 1105 2 -0.010163418059885743 1105 3 -0.01004278645807588 1105 4 -0.06773093453396939 1105 5 -0.0678661548891526 1105 6 0.0406471210017898 1105 7 0.06576510901444976 1105 8 0.08421319145750772 1105 9 0.17338156406901106 1105 10-11 -0.05635447318364584 1105 12-13 -0.03795439250495036 1105 14-15 0.09581334088567672 1105 16-17 -0.03279058875816787 1105 18-19 -0.0017603000669410562 1105 20-21 0.07514404004074748 1105 22-23 0.06190985370231061 1105 24-25 0.11391208185423096 1105 26-27 0.17733973413731263 1105 28-29 0.14944654519548806 1105 30-31 0.02910731843709158 1105 32-33 0.01151470707649338 1105 34-35 -0.06276592446840823 1105 36-37 -0.17029406762139132 1105 38-39 0.09751697267760306 1105 40-41 0.08079858745259116 1105 42-43 0.11978143184538936 1105 44-45 0.1890868762059199 1105 46-47 0.210135296700507 1105 48-49 0.247518737082018 1105 50-51 0.017234956828282577 1105 52-53 0.1127637760509046 1105 54-55 0.029864833553176595 1105 56-57 -0.02131733614126574 1105 58-59 0.04450972718618118 1105 60-61 0.2991446807692313 1105 62-63 0.21897459669855124 1105 64-65 0.3140681180182483 1105 66-67 0.1799509955574976 1105 68-69 0.2353899323196842 1105 70-71 0.3121537260548024 1105 72-73 0.3663966547828892 1105 74-75 0.2796083014594437 1105 76-77 0.27433891853233305 1105 78-79 0.12420726483468414 1105 80-81 0.3522044058483651 1105 82-83 0.34315015714343744 1105 84-85 0.42660949136581294 1105 86-87 0.31849303401002516 1105 88-89 0.30985571594635886 1105 90-91 0.521884110413815 1105 92-93 0.6180960051624886 1105 94-95 0.5189410079207804 1105 96-97 0.42700138147735345 1105 98-99 0.5360752491991541 1105 100-101 0.9729752016992279 1106 1 0.04076823525420892 1106 2 -0.10502775536897246 1106 3 -0.03372394341175067 1106 4 -0.028001212757345684 1106 5 -0.12726522379297478 1106 6 -0.1020930355099523 1106 7 0.05920194401912937 1106 8 -0.04142487930018035 1106 9 0.018205937039809328 1106 10-11 -0.29178511908423843 1106 12-13 -0.39522629819813204 1106 14-15 -0.31015806028921133 1106 16-17 -0.2049674296958628 1106 18-19 -0.079858967842803 1106 20-21 -0.09739625766507487 1106 22-23 0.049445089258611574 1106 24-25 0.12089265216057754 1106 26-27 0.06769840048691123 1106 28-29 0.19976622013629708 1106 30-31 0.13008745874571304 1106 32-33 0.39453610648843807 1106 34-35 0.22687982580471555 1106 36-37 0.2965311573917475 1106 38-39 0.18585419905443956 1106 40-41 0.11790272510700106 1106 42-43 0.15056656474040864 1106 44-45 0.22373575867577244 1106 46-47 0.4579189659596139 1106 48-49 0.6428931598524912 1106 50-51 0.44550526580365357 1106 52-53 0.303240705029431 1106 54-55 0.45705497870523715 1106 56-57 0.33398180256428134 1106 58-59 0.27503304395850847 1106 60-61 0.2694211544591738 1106 62-63 0.17941122730849912 1106 64-65 0.43833971327533305 1106 66-67 0.5561288831234279 1106 68-69 0.389191660952946 1106 70-71 0.601795632328546 1106 72-73 0.4574680251262535 1106 74-75 0.3266593839694316 1106 76-77 0.6236894019351347 1106 78-79 0.5960277007548136 1106 80-81 0.6112323034749743 1106 82-83 0.49650956074561137 1106 84-85 0.4204075297596752 1106 86-87 0.5747174631297263 1106 88-89 0.6430616670936296 1106 90-91 0.5447352286067257 1106 92-93 0.6758950424659851 1106 94-95 0.7323007349491064 1106 96-97 0.7641180055021337 1106 98-99 1.143567256994256 1106 100-101 0.9050200529536845 1107 1 -0.1008892593444628 1107 2 -0.1644118000471373 1107 3 -0.12865484716618525 1107 4 -0.1534445293771327 1107 5 -0.07511692164467121 1107 6 -0.11168294215868713 1107 7 -0.20595235054693006 1107 8 0.025704467240927897 1107 9 0.07939382175710819 1107 10-11 -0.019583618941560132 1107 12-13 0.01667089311082748 1107 14-15 -0.15179721075188723 1107 16-17 -0.0477836622179737 1107 18-19 -0.050263761777003424 1107 20-21 -0.19146600614659093 1107 22-23 -0.16945140904964973 1107 24-25 -0.11142563898123825 1107 26-27 -0.03859567639539563 1107 28-29 -0.10456299595762886 1107 30-31 0.027421444314995824 1107 32-33 -0.12908196860022514 1107 34-35 -0.05880461429148909 1107 36-37 -0.21335938926350195 1107 38-39 -0.14240392332133212 1107 40-41 -0.1320106498063751 1107 42-43 -0.2562401288309957 1107 44-45 -0.04436378883553971 1107 46-47 0.06608822118769808 1107 48-49 0.04772276321150315 1107 50-51 0.02335574071295099 1107 52-53 0.018534618852761753 1107 54-55 0.07806512912716101 1107 56-57 0.0421164077577032 1107 58-59 0.07024114799014569 1107 60-61 0.06895032163540549 1107 62-63 0.10424103449124544 1107 64-65 0.04254969248531637 1107 66-67 0.06404233956629923 1107 68-69 0.011169974107737346 1107 70-71 0.013397279224321323 1107 72-73 -0.18517291898528043 1107 74-75 -0.2078021137273609 1107 76-77 -0.19489218870527836 1107 78-79 0.002095533293235974 1107 80-81 0.047252033612348754 1107 82-83 0.12208536279200288 1107 84-85 0.20025241989868547 1107 86-87 0.12130647295402852 1107 88-89 0.07188641944779661 1107 90-91 0.20387570392367138 1107 92-93 0.3284163420461077 1107 94-95 0.3230634433588726 1107 96-97 0.5992449876879391 1107 98-99 0.6896467074854691 1107 100-101 0.7764684244020579 1108 1 0.011508675729452023 1108 2 -0.00473835195348471 1108 3 -0.09566646879897789 1108 4 -0.04928915050385996 1108 5 -0.008623207246607478 1108 6 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2316 60-61 -0.790239916892375 2316 62-63 -1.1365493798007478 2316 64-65 -0.9868883539085687 2316 66-67 -1.0928411576168813 2316 68-69 -0.8353757344253623 2316 70-71 -1.2730114906229417 2316 72-73 -1.291965254878562 2316 74-75 -1.1510747056592585 2316 76-77 -1.6060582990038803 2316 78-79 -1.507751853525761 2316 80-81 -1.370912673195889 2316 82-83 -1.3956222258935078 2316 84-85 -1.3992584899434561 2316 86-87 -1.705548560919933 2316 88-89 -1.7485405957741698 2316 90-91 -1.8109814548609329 2316 92-93 -2.0375109781398884 2316 94-95 -1.8296481131381732 2316 96-97 -2.30435522742647 2316 98-99 -2.755595033891918 2316 100-101 -3.046801642558865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6880.0 3 1530.0 4 1437.0 5 1469.0 6 1581.0 7 1863.0 8 2260.0 9 2848.0 10 3511.0 11 4213.0 12 5416.0 13 6885.0 14 7723.0 15 9440.0 16 11721.0 17 14977.0 18 12929.0 19 9282.0 20 9141.0 21 9569.0 22 10357.0 23 11504.0 24 12891.0 25 14602.0 26 17055.0 27 19842.0 28 23749.0 29 28334.0 30 34888.0 31 43330.0 32 55006.0 33 72170.0 34 98512.0 35 140565.0 36 206626.0 37 293563.0 38 240260.0 39 31759.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.679778328731004 18.950967573931113 14.767844645656902 22.60140945168098 2 15.073921410332797 20.256926976044596 40.335921989204785 24.333229624417825 3 19.095303680166577 24.645173272024333 29.920738419160507 26.338784628648582 4 12.367481345711683 16.56273046722485 37.84597197518546 33.22381621187801 5 13.610768608189385 37.261252491948824 34.33964796499037 14.788330934871421 6 31.37106420678603 36.463838047773415 17.652294183228506 14.512803562212051 7 28.45530933026133 32.70396549820834 20.738120538196526 18.102604633333808 8 26.441554536163398 34.35795564989411 19.923073745038025 19.27741606890447 9 26.169304701869965 16.909272899165924 20.68577628125076 36.23564611771335 10-11 24.7038417924481 26.794155143005394 27.500342085761236 21.001660978785264 12-13 25.911879270862332 24.073829489938223 28.053092969590814 21.96119826960863 14-15 22.54523584814612 25.43535895250393 26.710628396731078 25.308776802618876 16-17 21.863541112136076 29.028652045345872 27.038505249669143 22.06930159284891 18-19 21.67678746725117 27.211199938777426 29.82595925244797 21.286053341523434 20-21 22.39760515780909 26.178095564117594 29.921712551894515 21.50258672617881 22-23 22.044042034120977 26.232115436382507 29.86836825736503 21.855474272131488 24-25 22.203371467458467 26.373556427558142 29.83813028802042 21.58494181696297 26-27 21.840088031149484 26.706890130353816 29.766141865583208 21.68687997291349 28-29 21.46907157147327 26.84441578882494 30.020019803079904 21.66649283662188 30-31 21.578054518933357 26.461213960515444 29.999502150405444 21.961229370145755 32-33 21.49925125823159 26.696318330729163 29.823221086410424 21.98120932462883 34-35 22.21796996764298 26.556567893189985 29.504159269437075 21.72130286972996 36-37 21.934850819570553 26.410957809494494 29.711790026332036 21.942401344602917 38-39 21.68717246198883 26.796782365444898 29.803546603085046 21.712498569481227 40-41 21.894882677811577 26.61512688347955 29.77705669331008 21.7129337453988 42-43 21.721342143712107 26.667005266927053 29.38224075494314 22.2294118344177 44-45 21.870553537022676 26.89664396804811 29.150918159684952 22.08188433524426 46-47 21.960741685900977 27.104734891583497 28.807479438249732 22.12704398426579 48-49 22.134675144878262 27.131518844333424 28.631337323186212 22.102468687602105 50-51 21.835199751821545 27.278429268801517 28.67158824051364 22.2147827388633 52-53 22.264322865945054 27.400654091799804 28.22861710902264 22.106405933232505 54-55 21.867994023372788 27.192502394308388 28.4164782602217 22.52302532209713 56-57 22.0441669471057 27.60197203373935 28.298250805260654 22.05561021389429 58-59 22.298045801113204 27.155272815915275 28.359041724787044 22.187639658184477 60-61 22.170808589958565 27.143621769904136 28.801237448037643 21.884332192099656 62-63 21.975773958901865 27.23354131144033 28.838340472846603 21.952344256811205 64-65 21.86983920843995 27.230359114918535 28.62491967870126 22.274881997940255 66-67 21.797077938015615 27.532327896290738 28.26766756729216 22.40292659840149 68-69 21.76043283149579 27.302988817662083 28.65331908797188 22.28325926287025 70-71 21.914347363420877 27.327877583177845 28.439646652656553 22.318128400744726 72-73 21.996916320142034 27.113427820967807 28.65184821036195 22.23780764852821 74-75 21.736969360844274 27.544530231326625 28.35267178002081 22.36582862780829 76-77 21.905333005119925 27.33355161556504 28.326013429436202 22.435101949878835 78-79 21.876198818390417 27.4639749254172 28.311599838695972 22.34822641749641 80-81 21.771455097226674 27.776414028432423 28.14046174333214 22.31166913100876 82-83 21.9184584403748 27.527860123154678 28.214121754392142 22.33955968207838 84-85 21.811646234349922 27.47659957229411 28.014397242424334 22.69735695093163 86-87 21.86288038002795 27.35469320434627 28.3519889718229 22.430437443802877 88-89 21.84226589374637 27.280818492940835 28.094104615076283 22.78281099823652 90-91 21.74876923175888 27.296562245335675 28.325257994957415 22.62941052794803 92-93 22.02149394604678 27.094491774308487 28.349360412071682 22.534653867573056 94-95 21.709514265982367 27.037455820818717 28.305584519344052 22.947445393854863 96-97 21.72997966075989 26.97820099285825 28.37686228628225 22.91495706009961 98-99 21.65394013490925 27.161871123509034 28.086253077238478 23.097935664343243 100-101 21.637203791469194 26.865707515233584 28.55365605958023 22.943432633716995 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2131.0 1 1213.5 2 365.5 3 440.0 4 367.0 5 349.5 6 629.5 7 1360.0 8 2222.0 9 2552.5 10 2226.5 11 1868.0 12 1891.5 13 2072.5 14 2235.0 15 2304.0 16 2389.5 17 2445.0 18 2464.0 19 2623.5 20 2945.0 21 3406.5 22 3983.0 23 4717.5 24 5767.0 25 7155.0 26 8978.0 27 11086.5 28 12735.0 29 14476.5 30 16925.5 31 19461.5 32 22220.0 33 25486.0 34 29473.5 35 32578.0 36 35463.5 37 39022.5 38 41487.0 39 42263.0 40 42959.5 41 45836.5 42 49088.5 43 51688.0 44 53707.5 45 55212.0 46 55155.5 47 57043.0 48 63907.5 49 64505.5 50 59542.5 51 56099.0 52 51956.5 53 49758.0 54 47783.0 55 43450.5 56 38462.5 57 33494.0 58 29154.5 59 25232.0 60 21534.0 61 17350.5 62 13419.0 63 10345.5 64 7803.5 65 5939.5 66 4376.0 67 3350.5 68 2371.5 69 1523.5 70 1162.0 71 903.5 72 766.5 73 606.0 74 413.5 75 331.0 76 247.5 77 146.0 78 87.0 79 58.0 80 37.0 81 28.0 82 18.0 83 10.0 84 10.0 85 11.5 86 9.0 87 8.5 88 8.5 89 4.0 90 5.0 91 7.0 92 7.0 93 6.5 94 6.5 95 5.0 96 4.0 97 6.0 98 8.5 99 6.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34331376174565587 2 0.29823693515424793 3 0.2924925119754328 4 0.299520982688336 5 0.30039954152744885 6 0.22369459672797656 7 0.2118678431245337 8 0.21740952195586122 9 0.2089618408105449 10-11 0.2336966512040311 12-13 0.22078859441398777 14-15 0.21521312485807895 16-17 0.21774742920167384 18-19 0.2106175863150269 20-21 0.204264930093749 22-23 0.20534623328034945 24-25 0.1953779695288762 26-27 0.1990949492328154 28-29 0.2134222164552719 30-31 0.22616131962240896 32-33 0.21754468485418627 34-35 0.2267357619402904 36-37 0.20122376488143512 38-39 0.19997350807192826 40-41 0.19652685416463922 42-43 0.20467041878872416 44-45 0.20957007385300763 46-47 0.2140980309468972 48-49 0.22193747904975075 50-51 0.22565445875368995 52-53 0.2435297520571793 54-55 0.22153199035477555 56-57 0.19226922286739978 58-59 0.19436424779143824 60-61 0.1882481266422292 62-63 0.19855429763951513 64-65 0.19024177939252387 66-67 0.18649100896400342 68-69 0.18409186751873358 70-71 0.19635790054173288 72-73 0.19524280663055113 74-75 0.20102102053394752 76-77 0.20372427850044875 78-79 0.20149409067808524 80-81 0.20048036894064727 82-83 0.19287745590986258 84-85 0.18561245012489053 86-87 0.18598414809528444 88-89 0.18831570809139173 90-91 0.19426287561769445 92-93 0.18709924200646622 94-95 0.18625447389193456 96-97 0.1857138222986343 98-99 0.1977095295249835 100-101 0.18219958694218272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1479696.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.61500743052616 #Duplication Level Percentage of deduplicated Percentage of total 1 80.36475678504983 46.30216059323074 2 11.511721283581796 13.26495814583423 3 3.3882618528574002 5.8564419548684254 4 1.4767364865965342 3.4032873459275375 5 0.8181880415147204 2.3569955045719135 6 0.4706628936678585 1.6270347669567968 7 0.33226175621976856 1.3400284487437149 8 0.23651605034286718 1.0901499198354394 9 0.1741077163776546 0.9028095635529463 >10 1.0685407623710244 11.699996547439387 >50 0.10089078278529284 4.060370738823037 >100 0.053602335392498944 5.414817456318169 >500 0.0028149399320589307 1.0425650035644098 >1k 7.037349830147327E-4 0.8139548527309642 >5k 2.3457832767157758E-4 0.824429157602139 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6975 0.4713806079086515 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5225 0.3531130718742228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3802 0.25694466971594165 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2202 0.14881435105589255 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2139 0.14455671975865314 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1504 0.10164249954044614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.379072458126534E-5 0.0 12-13 0.0 0.0 0.0 6.758144916253068E-5 0.0 14-15 0.0 0.0 0.0 6.758144916253068E-5 0.0 16-17 0.0 0.0 0.0 6.758144916253068E-5 0.0 18-19 0.0 0.0 0.0 6.758144916253068E-5 0.0 20-21 0.0 0.0 0.0 6.758144916253068E-5 0.0 22-23 0.0 0.0 0.0 1.3516289832506137E-4 0.0 24-25 0.0 0.0 0.0 1.689536229063267E-4 0.0 26-27 0.0 0.0 0.0 3.379072458126534E-4 0.0 28-29 0.0 0.0 0.0 5.406515933002455E-4 0.0 30-31 0.0 0.0 0.0 0.0015205826061569403 0.0 32-33 0.0 0.0 0.0 0.002669467241919962 0.0 34-35 0.0 0.0 0.0 0.0036831889793579225 0.0 36-37 0.0 0.0 0.0 0.006014748975465231 0.0 38-39 0.0 0.0 0.0 0.008650425492803928 0.0 40-41 0.0 0.0 0.0 0.012536358819649441 0.0 42-43 0.0 0.0 0.0 0.0161857570744261 0.0 44-45 0.0 0.0 0.0 0.018314572723045815 0.0 46-47 0.0 0.0 0.0 0.021727435905753614 0.0 48-49 0.0 0.0 0.0 0.026998788940431007 0.0 50-51 0.0 0.0 0.0 0.033182491538802567 0.0 52-53 0.0 0.0 0.0 0.05048334252441042 0.0 54-55 0.0 0.0 0.0 0.06153290946248419 0.0 56-57 0.0 0.0 0.0 0.09113358419567263 0.0 58-59 0.0 0.0 0.0 0.13796752846530638 0.0 60-61 0.0 0.0 0.0 0.18392291389582727 0.0 62-63 0.0 0.0 0.0 0.21365875152734076 0.0 64-65 0.0 0.0 0.0 0.263229744488057 0.0 66-67 0.0 0.0 0.0 0.3494974643440274 0.0 68-69 0.0 0.0 0.0 0.4709751192136763 0.0 70-71 0.0 0.0 0.0 0.6009680366778041 0.0 72-73 0.0 0.0 0.0 0.7369081216682346 0.0 74-75 0.0 0.0 0.0 0.8993063440057958 0.0 76-77 0.0 0.0 0.0 1.0345368237800199 0.0 78-79 0.0 0.0 0.0 1.2070384727673793 0.0 80-81 0.0 0.0 0.0 1.4328280944193943 0.0 82-83 0.0 0.0 0.0 1.6433443085606774 0.0 84-85 0.0 0.0 0.0 1.8873133400374131 0.0 86-87 0.0 0.0 0.0 2.1975797731425915 0.0 88-89 0.0 0.0 0.0 2.595735880883641 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8845 0.0 30.058956 1 GGTATCA 5970 0.0 26.688883 1 GTACATG 6440 0.0 26.292025 1 GCGTTCG 55 9.469661E-5 25.91388 34-35 ATCAACG 10465 0.0 25.257431 3 TCAACGC 10480 0.0 25.224705 4 TATCAAC 10560 0.0 25.165268 2 CAACGCA 10535 0.0 25.09046 5 AACGCAG 10715 0.0 24.647232 6 TACATGG 6545 0.0 24.40525 2 ACATGGG 6585 0.0 23.101908 3 GAGTACT 5455 0.0 22.856272 12-13 ACGCAGA 11865 0.0 22.058167 7 CGCAGAG 11880 0.0 21.951094 8 GTACTTT 5765 0.0 21.62613 14-15 CGAACCG 55 0.0028792997 21.589777 76-77 AGTACTT 5685 0.0 21.513824 12-13 GCAGAGT 12350 0.0 20.6157 9 GTACCTG 1275 0.0 20.143896 1 AGAGTAC 9325 0.0 20.043865 10-11 >>END_MODULE